CN103222554A - Compound Chinese herbal medicine additive for sea cucumber and preparation method thereof - Google Patents
Compound Chinese herbal medicine additive for sea cucumber and preparation method thereof Download PDFInfo
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- CN103222554A CN103222554A CN2012105651118A CN201210565111A CN103222554A CN 103222554 A CN103222554 A CN 103222554A CN 2012105651118 A CN2012105651118 A CN 2012105651118A CN 201210565111 A CN201210565111 A CN 201210565111A CN 103222554 A CN103222554 A CN 103222554A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a compound Chinese herbal medicine additive for sea cucumber and a preparation method thereof. The compound Chinese herbal medicine additive can improve medicine effects, does not damage sea cucumber, does not produce residues in sea cucumber, does not influence sea cucumber normal growth, can obviously improve sea cucumber immunity, can promote sea cucumber growth, can kill bacteria, can overcome defects of antibiotics and chemical sanitizers, has no toxic and side effect and no corrosivity, is safe in use, can be degraded under natural conditions, does not pollute the water environment and is a product for effectively preventing sea cucumber diseases. Through reasonable compatibility, toxicity of natural Chinese herbal medicines in the compound Chinese herbal medicine additive can be reduced or eliminated and Chinese herbal medicine effects are improved. The compound Chinese herbal medicine additive has the advantages of stable ingredient sources, low cost, low price, simple and feasible processes and wide market prospects.
Description
Technical field
The present invention relates to a kind of preparation method of additive and this additive of holothurian feed, particularly a kind of trepang compound Chinese herb feed additive and preparation method thereof.
Background technology
Along with to the understanding in depth of sea cucumber nutrient health care, in continuous rising, get up by sea cucumber consumption market also day by day prosperity to the degree of recognition of sea cucumber for people, and meanwhile the holothruian cultures industry is also in swift and violent development.But, continuous development along with the holothruian cultures industry, the disease problem that is run in the breed also becomes increasingly conspicuous out, the disease of sea cucumber was since fragmentary generation in 2002, in the period of 2003 to 2005, worsen fast, the various illnesss and the large-scale phenomena of mortality have occurred, caused great economic loss, seriously hindered the healthy and sustainable development of holothruian cultures industries to aquaculture.The common self-dissolving (being commonly called as " change plate ") that has the mashed limit of auricularia in seedling stage, skin ulcer to cause; The putrid skin disease that the mature stage sea cucumber occurs, swollen mouth disease or the like.At present, the sea cucumber prevention and treatment of diseases mostly adopts the antibiotic dipping to handle, and especially the growing seedlings and indoor keeping a full stand of seedings the stage of sea cucumber, but along with the resistance to the action of a drug of bacterium, virus strengthens gradually, in order to treat disease, therefore just must strengthen dosage.And the use of excessive antibiotic and chemicals can cause the antibiotic residue in the sea cucumber body to exceed standard, and not only influences ingesting and growing of sea cucumber, has also influenced the marketing quality of stichopus japonicus simultaneously, and people's edible safety is also constituted a serious threat; The use of other chemical classes disinfectant also can pollute water body, contaminated environment.
Summary of the invention
The present invention is in order to solve the above-mentioned deficiency of existing in prior technology, the production method of a kind of holothurian feed additive and this additive is proposed, characteristics such as this additive has natural sex, noresidue, the no resistance to the action of a drug, price is low, the source is abundant, it can not only significantly improve the immunity of organisms of sea cucumber, strengthen premunition, can also significantly promote the sea cucumber growth.
Technical solution of the present invention is: a kind of trepang compound Chinese herb feed additive is characterized in that: each weight percentages of components of described Chinese herb feed additive is as follows:
Radix Astragali 15%-25%
Folium isatidis 15%-25%
Herba Andrographitis 15%-25%
Radix Angelicae Sinensis 10%-20%
Dried orange peel 5%-15%
Stir-baked FRUCTUS CRATAEGI 5%-15%
Honeysuckle 5%-10%
A kind of method for preparing trepang compound Chinese herb feed additive as claimed in claim 1 is characterized in that: the Chinese herbal medicine of getting following component:
Radix Astragali 15%-25%
Folium isatidis 15%-25%
Herba Andrographitis 15%-25%
Radix Angelicae Sinensis 10%-20%
Dried orange peel 5%-15%
Stir-baked FRUCTUS CRATAEGI 5%-15%
Honeysuckle 5%-10%
Said herbal medicine is dried under 50 to 70 degrees celsius, and pulverize, get the screenings of mixing after sieving by sample.
The described step of sieving is to cross 60 mesh sieves, 120 mesh sieves successively, gets the screenings of mixing.
The described step of sieving was 100 mesh sieves, get sieve down Chinese herbal medicine solid mixt and the pure water of mixing mix according to the mass volume ratio of 1g:40mL to 80mL and soak, soak and be heated to boiling after 10 to 14 hours, change little fire to boil, be concentrated into original volume
, leach soup with 100 mesh sieve tulles then, obtain the Chinese herbal medicine annex solution.
The present invention compares with prior art, has following advantage:
Holothurian feed Chinese herb feed additive of the present invention when strengthening drug effect, to not damage of sea cucumber, and can not produce residually in the sea cucumber body, does not influence the normal growth of sea cucumber, and is quite remarkable to the raising effect of sea cucumber immunity.And it can also play the effect of growth promotion and sterilization when regulating and improving sea cucumber immunity, thereby has eliminated the weak point of using antibiotics and chemical disinfection similar drug.It has no side effect, non-corrosiveness, and is safe in utilization, and degradable is also pollution-free to water environment under field conditions (factors), is the product that effectively prevents the sea cucumber disease.And its reasonable compatibility makes the perform toxic attenuation or the elimination of some natural Chinese medicinal herb that contain toxic component itself, better brings into play its useful effect.Component steady sources of the present invention, with low cost, low price, the production technology simple possible possesses extensive market prospects.
Description of drawings
Fig. 1 is the parameter influence schematic diagram that has used sea cucumber acid phosphatase behind the various embodiments of the present invention additive.
Fig. 2 is the parameter influence schematic diagram that has used sea cucumber alkaline phosphatase behind the various embodiments of the present invention additive.
Fig. 3 is the parameter influence schematic diagram that has used sea cucumber superoxide dismutase behind the various embodiments of the present invention additive.
Fig. 4 is the parameter influence schematic diagram that has used sea cucumber antalzyme behind the various embodiments of the present invention additive.
Fig. 5 has used behind the various embodiments of the present invention additive schematic diagram that sea cucumber was carried out in 30 days sea cucumber cumulative mortality behind the challenge test.
The specific embodiment
Below in conjunction with description of drawings the specific embodiment of the present invention.
Embodiment one
Get the 2 gram Radixs Astragali, 2 gram folium isatidis, 2 gram Herba Andrographitis, 1.5 gram Radix Angelicae Sinensis, 1 gram dried orange peel, 1 gram stir-baked FRUCTUS CRATAEGI, 0.5 gram honeysuckle, under 60 degrees centigrade condition, dry, pulverized 60 order mesh screens, dosage with feed weight 2% behind the screenings medicinal powder mixing adds in the sea cucumber basal feed, water and even is splashed at Miao Chizhong before throwing something and feeding.
Embodiment two
Get the 2 gram Radixs Astragali, 2 gram folium isatidis, 2 gram Herba Andrographitis, 1.5 gram Radix Angelicae Sinensis, 1 gram dried orange peel, 1 gram stir-baked FRUCTUS CRATAEGI, 0.5 gram honeysuckle, under 60 degrees centigrade condition, dry, pulverized 120 order mesh screens, dosage with feed weight 2% behind the screenings medicinal powder mixing adds in the sea cucumber basal feed, water and even is splashed at Miao Chizhong before throwing something and feeding.
Embodiment three
Get the 2 gram Radixs Astragali, 2 gram folium isatidis, 2 gram Herba Andrographitis, 1.5 gram Radix Angelicae Sinensis, 1 gram dried orange peel, 1 gram stir-baked FRUCTUS CRATAEGI, 0.5 gram honeysuckle, under 60 degrees centigrade condition, dry, pulverized 100 order mesh screens, screenings medicinal powder is put into the 600ml pure water to be soaked 12 hours, then mixing material is heated to boiling, changeing little fire boils, be concentrated into 200ml, crossing 100 mesh sieve tulles then filters, obtain the Chinese herbal medicine annex solution, calculate the volume (promptly as 1000 gram basal feeds, then the volume of annex solution is 10 milliliters) of annex solution according to 1% of sea cucumber basal feed weight, and add water and even in the foundation bait to, splash at Miao Chizhong.
Embodiment four
Get the 2 gram Radixs Astragali, 2 gram folium isatidis, 2 gram Herba Andrographitis, 1.5 gram Radix Angelicae Sinensis, 1 gram dried orange peel, 1 gram stir-baked FRUCTUS CRATAEGI, 0.5 gram honeysuckle, under 60 degrees centigrade condition, dry, pulverized 100 order mesh screens, screenings medicinal powder is put into the 600ml pure water to be soaked 12 hours, then mixing material is heated to boiling, changeing little fire boils, be concentrated into 200ml, crossing 100 mesh sieve tulles then filters, obtain the Chinese herbal medicine annex solution, calculate the volume (promptly as 1000 gram basal feeds, then the volume of annex solution is 20 milliliters) of annex solution according to 2% of sea cucumber basal feed weight, and add water and even in the foundation bait to, splash at Miao Chizhong.
Embodiment five
Get the 2 gram Radixs Astragali, 2 gram folium isatidis, 2 gram Herba Andrographitis, 1.5 gram Radix Angelicae Sinensis, 1 gram dried orange peel, 1 gram stir-baked FRUCTUS CRATAEGI, 0.5 gram honeysuckle, under 60 degrees centigrade condition, dry, pulverized 100 order mesh screens, screenings medicinal powder is put into the 600ml pure water to be soaked 12 hours, then mixing material is heated to boiling, changeing little fire boils, be concentrated into 200ml, crossing 100 mesh sieve tulles then filters, obtain the Chinese herbal medicine annex solution, calculate the volume (promptly as 1000 gram basal feeds, then the volume of annex solution is 20 milliliters) of annex solution according to 2% of sea cucumber basal feed weight, and add water and even in the foundation bait to, splash at Miao Chizhong.
Result of use is as follows:
Used the embodiment of the invention 1,2,3,4,5 influences to sea cucumber in back 30 days to see Table 1.
Control group wherein is the sea cucumber group that does not apply the Chinese herbal medicine feeding.The relative weight gain rate and the specific growth rate of the utility model embodiment 1,2,3,4,5 all are higher than control group, and wherein compare difference with control group extremely remarkable for the relative weight gain rate of embodiment 3,4 and specific growth rate.
As shown in Figure 1, through the sea cucumber that embodiment 1,2,3,4,5 handles, activity of acid phosphatase raises in time gradually, in the time of the 15th day and control group significant difference all.Wherein embodiment 3,4,5 is better than embodiment 1,2.And the performance of embodiment 4 is more outstanding.
As shown in Figure 2, the sea cucumber that embodiment 1,2,3,4,5 handles, alkaline phosphatase activities raises in time gradually, in the time of the 15th day and control group significant difference all.Wherein embodiment 3,4,5 is better than embodiment 1,2.And the performance of embodiment 4 is more outstanding.
As shown in Figure 3, the sea cucumber that embodiment 1,2,3,4,5 handles, superoxide dismutase activity descends in time gradually, in the time of the 2nd day and control group significant difference all.Wherein embodiment 3,4 is better than embodiment 1,2,5.And the performance of embodiment 4 is more outstanding.
As shown in Figure 4, the sea cucumber that embodiment 1,2,3,4,5 handles, lysozyme activity raises earlier gradually in time and afterwards reduces, in the time of the 10th day and control group significant difference all.And the performance of embodiment 4 is more outstanding.
As shown in Figure 5, the sea cucumber cumulative mortality that embodiment 1,2,3,4,5 handles all is lower than 50%, and control group has then reached 50%.Wherein embodiment 3 and 4 groups of cumulative mortalities will be lower than other each groups, and the cumulative mortality of embodiment 4 be minimum be 6.67%, be best one group of protection effect.
Claims (1)
1. trepang compound Chinese herb feed additive, it is characterized in that: each weight percentages of components of described Chinese herb feed additive is as follows:
Radix Astragali 15%-25%
Folium isatidis 15%-25%
Herba Andrographitis 15%-25%
Radix Angelicae Sinensis 10%-20%
Dried orange peel 5%-15%
Stir-baked FRUCTUS CRATAEGI 5%-15%
Honeysuckle 5%-10%;
A kind of method for preparing trepang compound Chinese herb feed additive as claimed in claim 1 is characterized in that: the Chinese herbal medicine of getting following component:
Radix Astragali 15%-25%
Folium isatidis 15%-25%
Herba Andrographitis 15%-25%
Radix Angelicae Sinensis 10%-20%
Dried orange peel 5%-15%
Stir-baked FRUCTUS CRATAEGI 5%-15%
Honeysuckle 5%-10%
Said herbal medicine is dried under 50 to 70 degrees celsius, and pulverize, get the screenings of mixing after sieving by sample;
The described step of sieving is to cross 60 mesh sieves, 120 mesh sieves successively, gets the screenings of mixing;
The described step of sieving was 100 mesh sieves, get sieve down Chinese herbal medicine solid mixt and the pure water of mixing mix according to the mass volume ratio of 1g:40mL to 80mL and soak, soak and be heated to boiling after 10 to 14 hours, change little fire to boil, be concentrated into original volume
, leach soup with 100 mesh sieve tulles then, obtain the Chinese herbal medicine annex solution.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609913A (en) * | 2013-12-04 | 2014-03-05 | 山东省海洋生物研究院 | Sea cucumber compound preparation for enhancing immunologic function of sea cucumber |
| CN104171735A (en) * | 2014-08-21 | 2014-12-03 | 青岛朗格生物技术有限公司 | Complete formula feed for feeding sea cucumber |
| CN104719201A (en) * | 2015-03-19 | 2015-06-24 | 山东省海洋生物研究院 | Multi-layer groove type apostichopus japonicus breeding method |
| CN104957390A (en) * | 2015-06-02 | 2015-10-07 | 柳州市明达鸽业养殖专业合作社 | Health-care sand for homing pigeons |
| CN109924345A (en) * | 2019-04-12 | 2019-06-25 | 生泰尔(内蒙古)科技有限公司 | A kind of holothurian feed pre-mixing agent and preparation method thereof using the preparation of Chinese medicine residue |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147531A (en) * | 2007-11-10 | 2008-03-26 | 大连理工大学 | Plant type apostichopus japonicus compound immunizing booster |
| CN101433268A (en) * | 2008-12-16 | 2009-05-20 | 大连水产学院 | Chinese herbal medicine feed additive for promoting growth of seedling of Apostichopus japonicus |
| CN101433269A (en) * | 2008-12-16 | 2009-05-20 | 大连水产学院 | Chinese herbal medicine feed additive capable of improving survival rate of Apostichopus japonicus |
| CN101658252A (en) * | 2009-09-17 | 2010-03-03 | 大连水产学院 | Food organism additive for cultivating stichopus japonicus |
| CN101744125A (en) * | 2010-02-02 | 2010-06-23 | 辽宁省农业科学院大连生物技术研究所 | Sea cucumber culturing feed additive and preparing and using methods thereof |
| CN101756022A (en) * | 2009-12-21 | 2010-06-30 | 大连金砣水产食品有限公司 | Holothurian regulation type feed additive |
| CN101971927A (en) * | 2010-10-12 | 2011-02-16 | 大连金砣水产食品有限公司 | Compound Chinese herb medicine addictive for culturing sea cucumber seedlings |
| CN102058020A (en) * | 2009-11-11 | 2011-05-18 | 大连水产学院 | Chinese medicinal herb additive for improving immunity of juvenile sea cucumber |
| CN102058722A (en) * | 2009-11-11 | 2011-05-18 | 大连水产学院 | Chinese herbal medicine fungicide special for holothurian culture |
| CN102326682A (en) * | 2011-07-14 | 2012-01-25 | 山东六和集团有限公司 | Feed additive for stichopus japonicus, preparation method and compound feed containing feed additive |
| CN102370839A (en) * | 2011-11-30 | 2012-03-14 | 刘正殿 | Medicament for treating sea cucumber disease |
-
2012
- 2012-12-24 CN CN2012105651118A patent/CN103222554A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147531A (en) * | 2007-11-10 | 2008-03-26 | 大连理工大学 | Plant type apostichopus japonicus compound immunizing booster |
| CN101433268A (en) * | 2008-12-16 | 2009-05-20 | 大连水产学院 | Chinese herbal medicine feed additive for promoting growth of seedling of Apostichopus japonicus |
| CN101433269A (en) * | 2008-12-16 | 2009-05-20 | 大连水产学院 | Chinese herbal medicine feed additive capable of improving survival rate of Apostichopus japonicus |
| CN101658252A (en) * | 2009-09-17 | 2010-03-03 | 大连水产学院 | Food organism additive for cultivating stichopus japonicus |
| CN102058020A (en) * | 2009-11-11 | 2011-05-18 | 大连水产学院 | Chinese medicinal herb additive for improving immunity of juvenile sea cucumber |
| CN102058722A (en) * | 2009-11-11 | 2011-05-18 | 大连水产学院 | Chinese herbal medicine fungicide special for holothurian culture |
| CN101756022A (en) * | 2009-12-21 | 2010-06-30 | 大连金砣水产食品有限公司 | Holothurian regulation type feed additive |
| CN101744125A (en) * | 2010-02-02 | 2010-06-23 | 辽宁省农业科学院大连生物技术研究所 | Sea cucumber culturing feed additive and preparing and using methods thereof |
| CN101971927A (en) * | 2010-10-12 | 2011-02-16 | 大连金砣水产食品有限公司 | Compound Chinese herb medicine addictive for culturing sea cucumber seedlings |
| CN102326682A (en) * | 2011-07-14 | 2012-01-25 | 山东六和集团有限公司 | Feed additive for stichopus japonicus, preparation method and compound feed containing feed additive |
| CN102370839A (en) * | 2011-11-30 | 2012-03-14 | 刘正殿 | Medicament for treating sea cucumber disease |
Non-Patent Citations (3)
| Title |
|---|
| 李正国等: "中药复方制剂的抗炎药理学研究", 《江苏农业科学》 * |
| 王印庚等: "中草药对刺参腐皮综合征病原菌的体外抑菌试验", 《渔业科学进展》 * |
| 黄玲等: "12种中药的体外抑菌试验", 《动物医学进展》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609913A (en) * | 2013-12-04 | 2014-03-05 | 山东省海洋生物研究院 | Sea cucumber compound preparation for enhancing immunologic function of sea cucumber |
| CN103609913B (en) * | 2013-12-04 | 2015-09-09 | 山东省海洋生物研究院 | A kind of Stichopus japonicus compound preparation for strengthening Stichopus japonicus immunologic function |
| CN104171735A (en) * | 2014-08-21 | 2014-12-03 | 青岛朗格生物技术有限公司 | Complete formula feed for feeding sea cucumber |
| CN104719201A (en) * | 2015-03-19 | 2015-06-24 | 山东省海洋生物研究院 | Multi-layer groove type apostichopus japonicus breeding method |
| CN104957390A (en) * | 2015-06-02 | 2015-10-07 | 柳州市明达鸽业养殖专业合作社 | Health-care sand for homing pigeons |
| CN109924345A (en) * | 2019-04-12 | 2019-06-25 | 生泰尔(内蒙古)科技有限公司 | A kind of holothurian feed pre-mixing agent and preparation method thereof using the preparation of Chinese medicine residue |
| CN109924345B (en) * | 2019-04-12 | 2022-05-10 | 生泰尔(内蒙古)科技有限公司 | Sea cucumber feed premix prepared from traditional Chinese medicine residues and preparation method thereof |
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Application publication date: 20130731 |