CN102058722A - Chinese herbal medicine fungicide special for holothurian culture - Google Patents
Chinese herbal medicine fungicide special for holothurian culture Download PDFInfo
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Abstract
The invention discloses a Chinese herbal medicine fungicide special for holothurian culture. Baical skullcap root is taken as a main raw material; rhubarb, Szechwan tangshen root, allicin, dutchmanspipe fruit, indigowoad root, burdock, tangerine peel, bupleurum, Chinese wolfberry, Chinese pulsatilla root, lightyellow sophora root, amur corktree bark, indigowoad leaf, oxytetracycline, sulfamonomethoxine, florfenicol and the like are added according to characteristics of pathogenic bacteria; the components are compatible with one another, and the Chinese herbal medicine fungicide has high attraction for apostichopus japonicus, and has the advantages of no pollution, no residues, no fungicide resistance, no side effect and the like; the Chinese herbal medicine fungicide is matched with the common feed to feed holothurian, has quick response, can kill the pathogenic bacteria, and has a remarkable effect on the prevention and control of the common cankered cutis disease, infectious keratodermia and the like of the holothurian so as to improve the survival rate of the apostichopus japonicus.
Description
Technical field:
The present invention relates to a kind of holothruian cultures antibacterial, especially a kind of pollution-free, noresidue, no Drug resistance, have no side effect, can suppress the generations of pathogenic bacterium and propagation, reduction beancurd sheet syndrome, change the sickness rate of plate disease etc., can improve the special-purpose Chinese herbal medicine antibacterial of holothruian cultures of survival rate of Apostichopus japonicus.
Background field:
The natural precious active substances of kind more than 50 such as Stichopus japonicus rich in proteins, mineral, vitamin have high nourishing and medical value, but allaying tiredness, improve body immunity etc.Acid mucopolysaccharide and chrondroitin can obviously reduce the quantity of lipofuscin and skin proline in the heart tissue in the Stichopus japonicus, play the old and feeble effect of continuity; 18 contained seed amino acids can strengthen metabolic function, the enhancing body cell viability of tissue in the Stichopus japonicus body, are suitable for the teenager in the growth promoter.
Exist a lot of problems in the holothruian cultures process at present, the most serious is exactly the disease problem of death of Radix Morinae Bulleyanae, as Radix Morinae Bulleyanae beancurd sheet syndrome, change plate disease etc.Radix Morinae Bulleyanae beancurd sheet syndrome during keep a full stand of seedings indoor wintering and outdoor ponds generation is all arranged during culturing, and the infection rate height, propagate fast, harm is serious, can cause the Radix Morinae Bulleyanae mortality; Change plate disease often occurs in young ginseng adheres in back 10～20 days, and this disease rapid onset, strong, the young ginseng mortality rate of infectiousness height are a kind of diseases that young ginseng initial stage breeding phase is the most general, harm is the most serious.For this reason, some are cultured the dealer and prevent and treat disease to add antibiotic in the pond of culturing Stichopus japonicus, add growth hormone etc. in the feedstuff for promoting Stichopus japonicus to be grown in fast, in the Stichopus japonicus body that causes growing up drug residue is arranged all, and the Radix Morinae Bulleyanae shoot survival percent are not high.
Summary of the invention:
The present invention is in order to solve the above-mentioned technical problem of existing in prior technology, a kind of pollution-free, noresidue, no Drug resistance are provided, have no side effect, can suppress the generations of pathogenic bacterium and propagation, reduction beancurd sheet syndrome, change the sickness rate of plate disease etc., can improve the special-purpose Chinese herbal medicine antibacterial of holothruian cultures of survival rate of Apostichopus japonicus.
Technical solution of the present invention is: the special-purpose Chinese herbal medicine antibacterial of a kind of holothruian cultures, comprise following component, each components by weight is: Radix Scutellariae 10～16, Radix Et Rhizoma Rhei 9～13, Radix Codonopsis 9～11, garlicin 8～10, Fructus Aristolochiae 7～10, Radix Isatidis 6～9, Fructus Arctii 4～7, Pericarpium Citri Reticulatae 4～6, Radix Bupleuri 3～8, Fructus Lycii 2～6, the Radix Pulsatillae 2～5, Radix Sophorae Flavescentis 2～5, Cortex Phellodendri 2～4 and Folium Isatidis 0.6～1.
Also contain following component in the said components, by weight: oxytetracycline 0.02～0.05, sulfamonomethoxine 0.04～0.07 and florfenicol 0.03～0.06.
The weight ratio of described component is: Radix Scutellariae 10～13, Radix Et Rhizoma Rhei 10～13, Radix Codonopsis 9～10, garlicin 8～9, Fructus Aristolochiae 8～10, Radix Isatidis 6～7, Fructus Arctii 5～7, Pericarpium Citri Reticulatae 4～6, Radix Bupleuri 3～8, Fructus Lycii 3～6, the Radix Pulsatillae 2～5, Radix Sophorae Flavescentis 3～5, Cortex Phellodendri 2～4, Folium Isatidis 0.6～0.8, oxytetracycline 0.03, sulfamonomethoxine 0.06 and florfenicol 0.03.
The weight ratio of described component is: 0.04 part of Radix Scutellariae 10～13, Radix Et Rhizoma Rhei 9～11, Radix Codonopsis 9～11, garlicin 8～9, Fructus Aristolochiae 8～10, Radix Isatidis 6～9, Fructus Arctii 5～6, Pericarpium Citri Reticulatae 4～5, Radix Bupleuri 5～8, Fructus Lycii 3～5, the Radix Pulsatillae 2～4, Radix Sophorae Flavescentis 3～5, Cortex Phellodendri 2～3, Folium Isatidis 0.6～0.8, oxytetracycline 0.02, sulfamonomethoxine 0.07 and florfenicol.
The weight ratio of described component is: 0.06 part of Radix Scutellariae 12～16, Radix Et Rhizoma Rhei 9～12, Radix Codonopsis 10～11, garlicin 8～10, Fructus Aristolochiae 7～10, Radix Isatidis 8～9, Fructus Arctii 4～6, Pericarpium Citri Reticulatae 4～6, Radix Bupleuri 3～6, Fructus Lycii 4～6, the Radix Pulsatillae 2～4, Radix Sophorae Flavescentis 3～5, Cortex Phellodendri 2～4, Folium Isatidis 0.8～1, oxytetracycline 0.05, sulfamonomethoxine 0.04 and florfenicol.
The weight ratio of described component is: 0.05 part of Radix Scutellariae 10～12, Radix Et Rhizoma Rhei 11～13, Radix Codonopsis 9～11, garlicin 8～10, Fructus Aristolochiae 7～10, Radix Isatidis 6～8, Fructus Arctii 4～7, Pericarpium Citri Reticulatae 4～6, Radix Bupleuri 3～8, Fructus Lycii 2～6, the Radix Pulsatillae 4～5, Radix Sophorae Flavescentis 2～4, Cortex Phellodendri 3～4, Folium Isatidis 0.7～0.9, oxytetracycline 0.04, sulfamonomethoxine 0.05 and florfenicol.
The present invention adopts Radix Scutellariae to make main raw material, according to the pathogen characteristics, add Radix Et Rhizoma Rhei, Radix Codonopsis, garlicin, Fructus Aristolochiae, Radix Isatidis, Fructus Arctii, Pericarpium Citri Reticulatae, Radix Bupleuri, Fructus Lycii, the Radix Pulsatillae, Radix Sophorae Flavescentis, Cortex Phellodendri, Folium Isatidis, oxytetracycline, sulfamonomethoxine and florfenicol etc., the mutual compatibility of each component, attractant to Radix Morinae Bulleyanae is good, has pollution-free, noresidue, no Drug resistance, advantage such as have no side effect; Be equipped with the normal diet Stichopus japonicus of feeding with the present invention, but rapid-action killing pathogenic bacteria has remarkable result to the common beancurd sheet syndrome of Stichopus japonicus, the control of changing plate disease etc., thereby improves the survival rate of Radix Morinae Bulleyanae.
The specific embodiment:
Embodiment 1:
Each component of raw material is by following weight ratio preparation, and each is below component micronizing to 300 order, and mixing gets final product.
Radix Scutellariae 10～13, Radix Et Rhizoma Rhei 10～13, Radix Codonopsis 9～10, garlicin 8～9, Fructus Aristolochiae 8～10, Radix Isatidis 6～7, Fructus Arctii 5～7, Pericarpium Citri Reticulatae 4～6, Radix Bupleuri 3～8, Fructus Lycii 3～6, the Radix Pulsatillae 2～5, Radix Sophorae Flavescentis 3～5, Cortex Phellodendri 2～4, Folium Isatidis 0.6～0.8, oxytetracycline 0.03, sulfamonomethoxine 0.06 and florfenicol 0.03.
Embodiment 2:
Each component of raw material is by following weight ratio preparation, and each is below component micronizing to 300 order, and mixing gets final product.
0.04 part of Radix Scutellariae 10～13, Radix Et Rhizoma Rhei 9～11, Radix Codonopsis 9～11, garlicin 8～9, Fructus Aristolochiae 8～10, Radix Isatidis 6～9, Fructus Arctii 5～6, Pericarpium Citri Reticulatae 4～5, Radix Bupleuri 5～8, Fructus Lycii 3～5, the Radix Pulsatillae 2～4, Radix Sophorae Flavescentis 3～5, Cortex Phellodendri 2～3, Folium Isatidis 0.6～0.8, oxytetracycline 0.02, sulfamonomethoxine 0.07 and florfenicol.
Embodiment 3:
Each component of raw material is by following weight ratio preparation, and each is below component micronizing to 300 order, and mixing gets final product.
0.06 part of Radix Scutellariae 12～16, Radix Et Rhizoma Rhei 9～12, Radix Codonopsis 10～11, garlicin 8～10, Fructus Aristolochiae 7～10, Radix Isatidis 8～9, Fructus Arctii 4～6, Pericarpium Citri Reticulatae 4～6, Radix Bupleuri 3～6, Fructus Lycii 4～6, the Radix Pulsatillae 2～4, Radix Sophorae Flavescentis 3～5, Cortex Phellodendri 2～4, Folium Isatidis 0.8～1, oxytetracycline 0.05, sulfamonomethoxine 0.04 and florfenicol.
Embodiment 4:
Each component of raw material is by following weight ratio preparation, and each is below component micronizing to 300 order, and mixing gets final product.
0.05 part of Radix Scutellariae 10～12, Radix Et Rhizoma Rhei 11～13, Radix Codonopsis 9～11, garlicin 8～10, Fructus Aristolochiae 7～10, Radix Isatidis 6～8, Fructus Arctii 4～7, Pericarpium Citri Reticulatae 4～6, Radix Bupleuri 3～8, Fructus Lycii 2～6, the Radix Pulsatillae 4～5, Radix Sophorae Flavescentis 2～4, Cortex Phellodendri 3～4, Folium Isatidis 0.7～0.9, oxytetracycline 0.04, sulfamonomethoxine 0.05 and florfenicol.
The pharmacological action of key component:
Radix Scutellariae, another name Radix Scutellariae, tsuchikane tea root.For labiate Radix Scutellariae (Scutellaria baicalensis Georgi), be used as medicine with root.Heat clearing and damp drying is arranged, and removing heat from blood is antiabortive, detoxicating functions.Be conical, distortion, long 8～25cm, diameter 1～3cm.Pale brown color in surface or buff have sparse excipuliform radicula trace, and top is more coarse, and the vertical wrinkle or the irregular reticulate pattern of distortion arranged, and rift grain and scabriculous are arranged at the bottom.Matter is hard and crisp, frangibility, and the section yellow, middle rufous is generally called sub-a kind of reed mentioned in ancient books, based on the yin nourishing of relieving inflammation or internal heat; Be burgundy or brownish black in the middle of the old root, dry and decayed shape or become the cavity claims Radix Scutellariae, relieves internal heat to relieve inflammation or internal heat.Feeble QI, bitter in the mouth.
Experimental example:
Carry out the Stichopus japonicus experiment of feeding in the experiment base.The experiment sea water is the natural water area tide sea water that rises and falls, after filtration and in the pond, continue inflation, every day a quantity of circulating water.The ammonia nitrogen of experimental water, nitrite, hydrogen sulfide, PH, dissolved oxygen equivalence are all in normal range.17～20 ℃ of water temperatures, salinity 29.5 ‰.Divide five zoness of different to compare test in same marine site, the experiment field is the industrialized culture booth, and identical feedstuff is thrown something and fed with equal number, the same terms.
When in culturing the pond, the skin fester disease phenomenon occurring, this is cultured the pond be divided into the separately breed of four ponds at random.Labelling is respectively A, B, C, D.A is a matched group, does not add the embodiment of the invention always; B just adds the embodiment of the invention 1 at the initial stage of a disease, carries out drug sensitive test simultaneously; C adds the embodiment of the invention 2 in morbidity mid-term, carries out drug sensitive test simultaneously; D adds the embodiment of the invention 3 in morbidity latter stage, carries out drug sensitive test simultaneously.The adding method is to be to add the embodiment of the invention at 100: 1 by mass ratio in feedstuff.
Experimental result shows that 1～3 pair of the embodiment of the invention causes that the pathogen of skin fester disease has stronger inhibitory or killing effect, simultaneously, find to culture B, C, D in the pond by continuing to observe, three groups of diseases do not continue infection, are effectively controlled and in six or seven month similar infection phenomenon do not take place again; Deadly and the two or three months morbidity once again again of beancurd sheet takes place in the diffusion of A group disease, Stichopus japonicus, and disease cycle prolongs four to five months.Finally cause A to form motility rate and be lower than other three groups more than 50%.
Utilize agar dilution to carry out drug sensitive test:
Get Stichopus japonicus with inoculating loop skin fester disease position antibacterial is taken place, preparation concentration is equivalent to the bacteria suspension of 0.5 Maxwell standard opacity tube, dilution in 1: 10 again, prepare bacterium liquid (about 1～2 μ l) with the absorption of multiple spot inoculator and be inoculated in agar plate surface, every some bacterium number is about 104CFU, and forming diameter is the bacterial plaque of 5～8mm.The good back of inoculation is put 35 ℃ with each concentration of aqueous solution of the embodiment of the invention and is hatched 24h, and observed result is determined the minimum inhibitory concentration (MIC) of the embodiment of the invention.
Experimental result:
Each group all obtains following result:
| Concentration (gL) | 9.00 | 7.80 | 6.60 | 6.45 | 6.30 | 6.15 | 6.00 | 5.50 | 4.00 | 3.30 |
| The B experimental group | - | - | - | - | (-) | + | + | + | + | + |
"-"-expression is not grown; "+"-expression growth; This concentration of " (-) "-expression is MIC.
Claims (6)
1. the special-purpose Chinese herbal medicine antibacterial of holothruian cultures, comprise following component, each components by weight is: Radix Scutellariae 10～16, Radix Et Rhizoma Rhei 9～13, Radix Codonopsis 9～11, garlicin 8～10, Fructus Aristolochiae 7～10, Radix Isatidis 6～9, Fructus Arctii 4～7, Pericarpium Citri Reticulatae 4～6, Radix Bupleuri 3～8, Fructus Lycii 2～6, the Radix Pulsatillae 2～5, Radix Sophorae Flavescentis 2～5, Cortex Phellodendri 2～4 and Folium Isatidis 0.6～1.
2. the special-purpose Chinese herbal medicine antibacterial of holothruian cultures according to claim 1 is characterized in that: also have following component, by weight: oxytetracycline 0.02～0.05, sulfamonomethoxine 0.04～0.07 and florfenicol 0.03～0.06.
3. the special-purpose Chinese herbal medicine antibacterial of holothruian cultures according to claim 1 is characterized in that the weight ratio of described component is: Radix Scutellariae 10～13, Radix Et Rhizoma Rhei 10～13, Radix Codonopsis 9～10, garlicin 8～9, Fructus Aristolochiae 8～10, Radix Isatidis 6～7, Fructus Arctii 5～7, Pericarpium Citri Reticulatae 4～6, Radix Bupleuri 3～8, Fructus Lycii 3～6, the Radix Pulsatillae 2～5, Radix Sophorae Flavescentis 3～5, Cortex Phellodendri 2～4, Folium Isatidis 0.6～0.8, oxytetracycline 0.03, sulfamonomethoxine 0.06 and florfenicol 0.03.
4. the special-purpose Chinese herbal medicine antibacterial of holothruian cultures according to claim 1 is characterized in that the weight ratio of described component is: 0.04 part of Radix Scutellariae 10～13, Radix Et Rhizoma Rhei 9～11, Radix Codonopsis 9～11, garlicin 8～9, Fructus Aristolochiae 8～10, Radix Isatidis 6～9, Fructus Arctii 5～6, Pericarpium Citri Reticulatae 4～5, Radix Bupleuri 5～8, Fructus Lycii 3～5, the Radix Pulsatillae 2～4, Radix Sophorae Flavescentis 3～5, Cortex Phellodendri 2～3, Folium Isatidis 0.6～0.8, oxytetracycline 0.02, sulfamonomethoxine 0.07 and florfenicol.
5. the special-purpose Chinese herbal medicine antibacterial of holothruian cultures according to claim 1 is characterized in that the weight ratio of described component is: 0.06 part of Radix Scutellariae 12～16, Radix Et Rhizoma Rhei 9～12, Radix Codonopsis 10～11, garlicin 8～10, Fructus Aristolochiae 7～10, Radix Isatidis 8～9, Fructus Arctii 4～6, Pericarpium Citri Reticulatae 4～6, Radix Bupleuri 3～6, Fructus Lycii 4～6, the Radix Pulsatillae 2～4, Radix Sophorae Flavescentis 3～5, Cortex Phellodendri 2～4, Folium Isatidis 0.8～1, oxytetracycline 0.05, sulfamonomethoxine 0.04 and florfenicol.
6. the special-purpose Chinese herbal medicine antibacterial of holothruian cultures according to claim 1 is characterized in that the weight ratio of described component is: 0.05 part of Radix Scutellariae 10～12, Radix Et Rhizoma Rhei 11～13, Radix Codonopsis 9～11, garlicin 8～10, Fructus Aristolochiae 7～10, Radix Isatidis 6～8, Fructus Arctii 4～7, Pericarpium Citri Reticulatae 4～6, Radix Bupleuri 3～8, Fructus Lycii 2～6, the Radix Pulsatillae 4～5, Radix Sophorae Flavescentis 2～4, Cortex Phellodendri 3～4, Folium Isatidis 0.7～0.9, oxytetracycline 0.04, sulfamonomethoxine 0.05 and florfenicol.
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| CN102058722B CN102058722B (en) | 2012-05-30 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102370839A (en) * | 2011-11-30 | 2012-03-14 | 刘正殿 | Medicament for treating sea cucumber disease |
| CN103222554A (en) * | 2012-12-24 | 2013-07-31 | 大连海洋大学 | Compound Chinese herbal medicine additive for sea cucumber and preparation method thereof |
| CN103463389A (en) * | 2013-08-30 | 2013-12-25 | 兴化市恒威生物技术有限公司 | Medicinal composition for treating skin ulceration syndrome of sea cucumber and preparation method thereof |
| CN106309866A (en) * | 2016-09-30 | 2017-01-11 | 威海虹润海洋科技有限公司 | Traditional Chinese medicine preparation for preventing and treating stichopus japonicas mouthpart fester diseases and skin ulcerative diseases and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101095721B (en) * | 2007-07-12 | 2011-10-05 | 辽宁省海洋水产科学研究院 | Sea cucumber Chinese medicinal herb preparations for preventing diseases and increasing the weight |
| CN101433269B (en) * | 2008-12-16 | 2012-07-18 | 大连水产学院 | Chinese herbal medicine feed additive capable of improving survival rate of Apostichopus japonicus |
-
2009
- 2009-11-11 CN CN200910219794XA patent/CN102058722B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102370839A (en) * | 2011-11-30 | 2012-03-14 | 刘正殿 | Medicament for treating sea cucumber disease |
| CN103222554A (en) * | 2012-12-24 | 2013-07-31 | 大连海洋大学 | Compound Chinese herbal medicine additive for sea cucumber and preparation method thereof |
| CN103463389A (en) * | 2013-08-30 | 2013-12-25 | 兴化市恒威生物技术有限公司 | Medicinal composition for treating skin ulceration syndrome of sea cucumber and preparation method thereof |
| CN106309866A (en) * | 2016-09-30 | 2017-01-11 | 威海虹润海洋科技有限公司 | Traditional Chinese medicine preparation for preventing and treating stichopus japonicas mouthpart fester diseases and skin ulcerative diseases and preparation method thereof |
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