CN1272260C - Modifying agent for microbiological drinking water for cultivated animal and making method thereof - Google Patents
Modifying agent for microbiological drinking water for cultivated animal and making method thereof Download PDFInfo
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- CN1272260C CN1272260C CN 200310107803 CN200310107803A CN1272260C CN 1272260 C CN1272260 C CN 1272260C CN 200310107803 CN200310107803 CN 200310107803 CN 200310107803 A CN200310107803 A CN 200310107803A CN 1272260 C CN1272260 C CN 1272260C
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Abstract
The present invention relates to a modifying agent for microbial drinking water for a cultured animal. The present invention is characterized in that the present invention is formed by that raw materials, such as a compound microbial strain, a liquid culture medium, inorganic salt, an inductive agent, sterilized water, etc. are fermented by complex liquid. A preparation method of the modifying agent for the microbial drinking water for the cultivated animal is characterized in that all used strains are selected from the intestinal tracts of animals or natural environment; all strains are finally cultivated in the same culture medium by a strain compounding segment cultivation method. Many selected strains, such as candida bacteria, acidophilus lactobacillus, rhodopseudomonas palustris, aspergillus niger, streptomyces, etc. survive and breed together by the optimized culture medium by a compound liquid fermentation method. The defects of large investment, low conversion rate, poor stability, etc. in a common liquid submerged fermentation method are overcome. Simultaneously, a product contains a large quantity of physiological metabolism substances, such as active enzymes, small peptide, antioxidants, free amino acid, vitamins, etc. The present invention has the advantage that the modifying agent purifies the potable water quality of livestock and poultry, inhibits the growth and the propagation of harmful pathogenic bacteria, enhances fodder conversion ratio and strengthens the immunity disease resistance of the livestock and the poultry.
Description
Technical field
The present invention relates to a kind of cultivated animals microorganism tap water modifying agent and making method, belong to cultivated animals tap water modifying agent technical field.
Background technology
Through the patent inquiry, also there is not the patent of relevant cultivated animals tap water modifying agent at present.In disclosed special agent, be the patent of feed additive field.Publication number is CN00133695, be entitled as biology feed additive and preparation method thereof, it is characterized in that: by screening lactobacillus viridescens, the pure bacterium of lactobacillus coprophilus, yeast, subtilis through enlarged culturing, secondary enlarged culturing, with rice bran, wheat bran adsorption liquid heap fermentation, oven dry and reach bacterial classification quantity 8-10 hundred million/gram.This biology feed additive zymotechnique is a single culture fermentation back remix, and viable count is low, and other physiological metabolism material yields poorly.
Summary of the invention
The purpose of this invention is to provide a kind of probiotics, to count height, enzymatic productivity strong and have a drinking water additive that is beneficial to the animal body Circulation.After use, when tap water is purified improvement,, also can form the optimum micro-flora of animal body by drinking of cultivated animals, impel conversion absorption, the enhance immunity power of nutritive substance.
For realizing above purpose, technical scheme of the present invention provides a kind of cultivated animals microorganism tap water modifying agent, it is characterized in that, the bacterial classification that is adopted all is from a kind of cultivated animals microorganism of cultivated animals enteron aisle tap water modifying agent, it is characterized in that, formed by the composite fluid fermentation by complex microorganism bacterial classification, liquid nutrient medium, inorganic salt, inductor, sterilized water raw material, it is prepared by following weight percentages:
Composite bacteria stoste 10-20%
Liquid nutrient medium 15-30%
Inorganic salt 0.1-5%
Inductor 0.02-1%
Sterilized water 45-65%,
Described composite bacteria is the mixture of candidiasis (Candida sp.), Lactobacterium acidophilum (Lactobacillusacidophilus), Rhodopseudomonas palustris (Rhodopseudomonas palustris), aspergillus niger (Aspergillus niger) and streptomyces (Streptomyces sp.).The optimum weight proportioning of each raw material of preparation of the present invention is:
Composite bacteria stoste 16%
Liquid nutrient medium 25%
Inorganic salt 1.2%
Inductor 0.1%
Sterilized water 57.7%.
Described liquid nutrient medium is the mixture of molasses, Zulkovsky starch, corn steep liquor and polypeptide class nutritive substance; Described inorganic salt are one of ammonium sulfate, sal epsom, ammonium nitrate, iron trichloride, sodium-chlor or their mixture; Described inductor is wooden sophorose, is mainly used in the output that improves organized enzyme.
Making method of the present invention, its characteristics are that all bacterial classifications of employing all filter out from animal intestinal or in the physical environment; Adopt the compound and segmentation cultural method of bacterial classification, all bacterial classifications are cultivated in same substratum, it comprises the following steps:
(1) strain screening from animal intestinal or physical environment utilizes protoplasma electric field integration technology to be optimized, and carries out purifying and cultivate;
(2) bacterial classification after the purifying cultivation is seeded in respectively in the same substratum by aerobic class and anaerobism class, component is molasses 1.5-2.5%, peptone 0.2-0.8%, glucose 0.1-0.5%, sodium-chlor 0.02-0.08%, yeast extract paste 0.8-1.2%, sal epsom 0.01-0.05%, carry out aerobic and the anaerobism cultivation, the ph value is to 6.5-7.0; Culture temperature is that 30-35 ℃, time are 48 hours; Aerobic and the anerobe liquid of then cultivation being finished mixes and adds glucose, lactose and polypeptide class nutritive substance and mixes, and blending ratio is 1: 0.12,0.08: 0.10, cultivates after 48 hours, obtains composite fungus agent;
(3) will insert in the closed tin behind composite fungus agent and liquid nutrient medium, inorganic salt, inductor and the sterilized water uniform mixing, stirred pot rotating speed 60r/min, 30-35 ℃ of environment stirs fermentation 72-96 hour down, obtains cultivated animals microorganism tap water modifying agent finished product.
The present invention adopts the bacterial classification by screening, optimization in multiple animal intestinal or the physical environment to mix with organic nutrient substance, make by microbial liquid complex ferment and segmentation culture technique, it contains physiological metabolism materials such as various active enzyme, total free aminoacids, monomeric protein, antioxidant, VITAMIN, and the quantity of probiotics viable count has reached 1,200,000,000/ml.
In breeding process, livestock and poultry animal to the demand of tap water be its forage volume 3-5 doubly, and at present domestic little ecological product of also not developing specially at the livestock and poultry tap water.The pollution of current because outside water surrounding causes livestock and poultry tap water in breeding process also to be subjected in various degree pollution, and causes livestock and poultry (particularly cub, poult) morbidity frequent, thereby caused a large amount of uses of chemical classes medicine.The present invention is directed to above problem, develop directly and in the livestock and poultry tap water, add complex microorganism preparations, in improvement water quality, also promote the growth of cultivated animals, feces of livestock and poultry has not had stink, reduced the generation and the propagation of pathogenic agent, alleviated a large amount of use medicines and caused residual environmental stress.
Characteristic of the present invention is not consider merely static nutritional needs; but from dynamic nutritional needs such as animal physilogical characteristics, production performance, environment protection and product safety, from---product safety---human health diversification system that develops into animal-environment protection of animal body independently.Rationally utilize the tap water modifying agent, guarantee animal production safety and human health, preserve the ecological environment.
The liquid state fermentation technology of the present invention's exploitation, utilize the substratum of optimizing, make a plurality of bacterial classification mutualisms such as candidiasis, Lactobacterium acidophilum, Rhodopseudomonas palustris, aspergillus niger and streptomyces that select, overcome big, the shortcomings such as transformation efficiency is low, poor stability of the existing investment of general liquid submerged fermentation method simultaneously.
The multiple microorganism species of in the enteron aisle of normal livestock and poultry, surviving, after the beneficial microorganism in the microorganism tap water modifying agent enters animal and bird intestines, in conjunction with original probiotics, form the superior microorganism flora rapidly, suppressed growth of harmful intestinal tract bacteria, beneficial microorganism is wherein combined closely by teichoic acid and intestinal epithelial cell around intestinal walls, forms the mycoderm barrier, stops the field planting of harmful bacterium; Also can promote the height response capacity of gut-associated lymphoid tissue simultaneously, make body fluid of animal and cellular immune level improve the enhancing body resistance against diseases.
Contained various active enzyme can be eliminated the multiple antinutritional factor in the feed in the cultivated animals microorganism tap water modifying agent, improves food conversion ratio, and promotes digesting and assimilating of nutritive substance.
Advantage of the present invention is:
1. the organic pollution materials in the decomposition tap water purifies the water quality of livestock and poultry tap water;
2. exogenous enzyme is provided, eliminates the antinutritional factor in the diet, improve food conversion ratio;
3. substitute the use of the chemical agents such as sterilizing agent that in the cultivated animals tap water, add, produce the organic farm products of green, safety;
4. suppress the growth of the harmful bacterium of gastrointestinal tract of livestock and fowls, promoted the height response capacity of gut-associated lymphoid tissue, strengthened the resistance against diseases and the immunological competence of livestock and poultry body.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail:
Embodiment
Bacterial classification source and assembly thereof:
Strain screening from animal intestinal or physical environment utilizes protoplasma electric field integration technology to be optimized, and carries out purifying and cultivate, and takes by weighing by following weight proportion:
Candidiasis (Candida sp.) 22%, Lactobacterium acidophilum (Lactobacillusacidophilus) 25%, Rhodopseudomonas palustris (Rhodopseudomonas palustris) 14%, aspergillus niger (Aspergillus niger) 19%, streptomyces (Streptomyces sp.) 20%
The making method of composite fungus agent:
1. take by weighing substratum by following weight proportion:
Molasses 1.9%, peptone 0.5%, glucose 0.3%, sodium-chlor 0.05%, yeast extract paste 1.0%, sal epsom 0.03%, the ph value is adjusted to 6.7;
2. the bacterial classification after purifying being cultivated is seeded in respectively in the same substratum by aerobic class and anaerobism class, component is molasses 2%, peptone 0.5%, glucose 0.3%, sodium-chlor 0.04%, yeast extract paste 1.0%, sal epsom 0.03%, carry out aerobic and the anaerobism cultivation, ph value to 6.7; Culture temperature is that 33 ℃, time are 48 hours; Aerobic and the anerobe liquid of then cultivation being finished mixes and adds glucose 2%, lactose 1.8% and polypeptide class nutritive substance 2.2% and mixes, and blending ratio is 1: 0.12,0.08: 0.10, cultivates after 48 hours, obtains composite fungus agent.
The making method of cultivated animals microorganism tap water modifying agent:
1. take by weighing composite fungus agent and raw material by following weight proportion:
Composite bacteria stoste 16%, liquid nutrient medium 25%, inorganic salt get that ammonium sulfate is 1.2%, inductor gets that wooden sophorose is 0.1%, sterilized water 57.7%;
2. will insert in the closed tin behind composite fungus agent and liquid nutrient medium, inorganic salt, inductor and the sterilized water uniform mixing, 33 ℃ of environment stir fermentation 82 hours down, and stirred pot rotating speed 60r/min obtains cultivated animals microorganism tap water modifying agent finished product.
In use, drink to livestock and poultry after utilizing liquid fillers to add tap water by different ratios, the fowl poultry kind animal is in 0.5-1 ‰ ratio.
Claims (6)
1. a cultivated animals microorganism tap water modifying agent is characterized in that, is formed by the composite fluid fermentation by complex microorganism bacterial classification, liquid nutrient medium, inorganic salt, inductor, sterilized water raw material, and it is prepared by following weight percentages:
Composite bacteria stoste 10-20%
Liquid nutrient medium 15-30%
Inorganic salt 0.1-5%
Inductor 0.02-1%
Sterilized water 45-65%,
Described composite bacteria is the mixture of candidiasis (Candida sp.), Lactobacterium acidophilum (Lactobacillusacidophilus), Rhodopseudomonas palustris (Phodopseudomonas palustris), aspergillus niger (Aspergillus niger) and streptomyces (Streptomyces sp.).
2. cultivated animals microorganism tap water modifying agent according to claim 1 is characterized in that wherein the optimum weight proportioning of each raw material is:
Composite bacteria stoste 16%
Liquid nutrient medium 25%
Inorganic salt 1.2%
Inductor 0.1%
Sterilized water 57.7%.
3. cultivated animals microorganism tap water modifying agent according to claim 1 is characterized in that, described liquid nutrient medium is the mixture of molasses, Zulkovsky starch, corn steep liquor and polypeptide class nutritive substance.
4. cultivated animals microorganism tap water modifying agent according to claim 1 is characterized in that described inorganic salt are one of ammonium sulfate, sal epsom, ammonium nitrate, iron trichloride, sodium-chlor or their mixture.
5. cultivated animals microorganism tap water modifying agent according to claim 1 is characterized in that described inductor is wooden sophorose.
6. the making method of cultivated animals microorganism tap water modifying agent according to claim 1 is characterized in that all bacterial classifications of employing all filter out from animal intestinal or in the physical environment; Adopt the compound and segmentation cultural method of many bacterial classifications, all bacterial classifications are cultivated in same substratum, it comprises the following steps:
(1) strain screening from animal intestinal or physical environment utilizes protoplasma electric field integration technology to be optimized, and carries out purifying and cultivate;
(2) bacterial classification after the purifying cultivation is seeded in respectively in the same substratum by aerobic class and anaerobism class, component is molasses 1.5-2.5%, peptone 0.2-0.8%, glucose 0.1-0.5%, sodium-chlor 0.02-0.08%, yeast extract paste 0.8-1.2%, sal epsom 0.01-0.05%, carry out aerobic and the anaerobism cultivation, the ph value is to 6.5-7.0; Culture temperature is that 30-35 ℃, time are 48 hours; Aerobic and the anerobe liquid mixing of then cultivation being finished and adding glucose 1-3%, lactose 1.6-2% and polypeptide class nutritive substance 2-2.5% mix, and blending ratio is 1: 0.12,0.08: 0.10, cultivates after 48 hours, obtains composite fungus agent;
(3) will insert in the closed tin behind composite fungus agent and liquid nutrient medium, inorganic salt, inductor and the sterilized water uniform mixing, mixing speed 60r/min stirs fermentation 72-96 hour under 30-35 ℃ of environment, obtain cultivated animals microorganism tap water modifying agent finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200310107803 CN1272260C (en) | 2003-10-08 | 2003-10-08 | Modifying agent for microbiological drinking water for cultivated animal and making method thereof |
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| CN 200310107803 CN1272260C (en) | 2003-10-08 | 2003-10-08 | Modifying agent for microbiological drinking water for cultivated animal and making method thereof |
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| CN1605571A CN1605571A (en) | 2005-04-13 |
| CN1272260C true CN1272260C (en) | 2006-08-30 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101891307B (en) * | 2010-07-12 | 2013-01-16 | 日照益康有机农业科技发展有限公司 | Marine culture biological cleanser and preparation method thereof |
| CN102250993A (en) * | 2011-07-18 | 2011-11-23 | 南京工业大学 | Optimized process for producing enramycin through fermenting streptomyces |
| CN102379363B (en) * | 2011-09-29 | 2013-02-20 | 滨州市正元畜牧发展有限公司 | Antibiotic-free culture method with combined application of drinking water strains and biological isolation |
| GB2496672B (en) * | 2011-11-21 | 2013-10-30 | Mclean Cleaning Ltd | Cleaning fluid comprising micro-organisms |
| CN105523594B (en) * | 2016-01-15 | 2018-07-03 | 绵阳金欧生物科技有限公司 | A kind of aquiculture waste water inorganic agent |
| CN109496969A (en) * | 2018-11-06 | 2019-03-22 | 南京雷德蒙德农业科技有限公司 | A kind of preventing control method of livestock and poultry cultivation disease |
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Denomination of invention: Modifying agent for microbiological drinking water for cultivated animal and making method thereof Effective date of registration: 20110616 Granted publication date: 20060830 Pledgee: Bank of Shanghai Limited by Share Ltd Minhang branch Pledgor: Shanghai Chuangbo Ecological Engineering Co., Ltd. Registration number: 2011990000218 |
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Denomination of invention: Modifying agent for microbiological drinking water for cultivated animal and making method thereof Effective date of registration: 20130106 Granted publication date: 20060830 Pledgee: Bank of Shanghai Limited by Share Ltd Minhang branch Pledgor: Shanghai Chuangbo Ecological Engineering Co., Ltd. Registration number: 2013990000012 |
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