CN107858305A - One plant of tetracycline efficient degradation bacterium and its application - Google Patents
One plant of tetracycline efficient degradation bacterium and its application Download PDFInfo
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- CN107858305A CN107858305A CN201711212339.8A CN201711212339A CN107858305A CN 107858305 A CN107858305 A CN 107858305A CN 201711212339 A CN201711212339 A CN 201711212339A CN 107858305 A CN107858305 A CN 107858305A
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- 239000004098 Tetracycline Substances 0.000 title claims abstract description 48
- 229960002180 tetracycline Drugs 0.000 title claims abstract description 48
- 229930101283 tetracycline Natural products 0.000 title claims abstract description 48
- 235000019364 tetracycline Nutrition 0.000 title claims abstract description 48
- 150000003522 tetracyclines Chemical class 0.000 title claims abstract description 47
- 230000015556 catabolic process Effects 0.000 title claims abstract description 45
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 45
- 241000894006 Bacteria Species 0.000 title claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000002351 wastewater Substances 0.000 claims abstract description 7
- 241000588748 Klebsiella Species 0.000 claims abstract description 5
- 238000000746 purification Methods 0.000 claims description 9
- 238000004321 preservation Methods 0.000 claims description 8
- 239000002054 inoculum Substances 0.000 claims description 7
- 230000003115 biocidal effect Effects 0.000 claims description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 abstract description 21
- 229940072172 tetracycline antibiotic Drugs 0.000 abstract description 8
- 238000004458 analytical method Methods 0.000 abstract description 4
- 230000000593 degrading effect Effects 0.000 abstract description 4
- 210000003608 fece Anatomy 0.000 abstract description 4
- 239000010871 livestock manure Substances 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 4
- 241000287828 Gallus gallus Species 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 238000013459 approach Methods 0.000 abstract description 2
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract description 2
- 239000003895 organic fertilizer Substances 0.000 abstract description 2
- 239000002028 Biomass Substances 0.000 abstract 1
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 238000013401 experimental design Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 13
- 229910052799 carbon Inorganic materials 0.000 description 13
- 238000000034 method Methods 0.000 description 9
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- 241000588747 Klebsiella pneumoniae Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 229940045505 klebsiella pneumoniae Drugs 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 241001673062 Achromobacter xylosoxidans Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000589539 Brevundimonas diminuta Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000588814 Ochrobactrum anthropi Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000005622 photoelectricity Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/22—Klebsiella
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
- C02F2103/343—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the pharmaceutical industry, e.g. containing antibiotics
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
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- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses one plant of tetracycline efficient degradation bacterium and its application, present invention screening from the organic fertilizer with chicken manure etc. for raw material fermentation obtains one plant of tetracycline efficient degradation bacterium, pass through physio-biochemical characteristics and 16SrDNA sequence analyses, the bacterial strain is accredited as Klebsiella, and is named as TR5.Under the efficiency laboratory level of strains for degrading tetracycline used in the present invention, by response surface experimental design, prediction can reach more than 90%.Data than being reported in current most of documents improve 20% or so.The present invention also improves capacity of resisting impact load and biomass of the tetracycline efficient degradation bacterium in water body using immobilized microorganism technology, is handled for related aquaculture system and pharmaceutical factory's tetracycline antibiotics waste water control aspect provides green, the biological approach of non-environmental-pollution.
Description
Technical field
The present invention relates to a kind of bacterial strain of tetracycline efficient degradation bacterium Klebsiella, more particularly to a kind of micro-
Application of the biological fixnig embedding techniques to water body purification of aquaculture and pharmaceutical factory's tetracycline antibiotics waste water treatment.
Background technology
In recent years, the environment tetracycline antibiotics caused by medical treatment and a large amount of uses of livestock and poultry breeding industry is dirty
Dye problem is on the rise, it has also become one of focus studied both at home and abroad.Studies have reported that claim the antibiotic in environment except that can make
Into outside chemical contamination, it is also possible to the generation of resistant microorganism and resistant gene in meeting induced environment, and aggravate antibiotic resistance biography
The risk broadcast.Some resistant microorganisms may directly or indirectly participate in the approach such as food chain and enter human body, cause human body
Antibiotic Resistance increase, so as to bring danger to a certain degree to coerce to mankind's publilc health.
Tetracycline is one of main species of tetracycline antibiotics, and existing many documents and patent report cross phase at present
The situation and method of tetracycline degraded are closed, is mainly urged including physico-chemical process, During High-Temperature Composting method, microbial degradation method, photoelectricity
Change etc..Wherein, microbial degradation method because its condition is single, be easily controlled, selectivity compared with it is strong, application cost is low and will not trigger
Secondary pollution, it is current both economical effective method.But in the prior art, the report to microbial degradation method and few, has
Document report degradeds of one Achromobacter xylosoxidans to tetracycline, degradation rate 63.9%, also document report one
Degraded of the bacillus subtilis to tetracycline, defect shortwave monad is mentioned in degradation rate 62.8%, also document
(Brevundimonas diminuta), degraded of the human pallid bacillus (Ochrobactrum anthropi) to tetracycline, but
Degradation efficiency is not high.Therefore the popularization and application of microbial degradation method be have impact on.
The content of the invention
It is an object of the invention to solve the deficiencies in the prior art, there is provided a kind of tetracycline efficient degradation bacterium, the bacterium
Nearly 30% is improved than prior art to the degradation rate of tetracycline, can be applied to culture fishery water body purification and tetracycline
Class antibiotic pharmacy sewage is administered.
Technical scheme
A kind of tetracycline efficient degradation bacterium, it is Friedlander's bacillus category (Klebsiella pneumoniae), hides
In China Microbial Culture Preservation Commission's common micro-organisms center, deposit number is CGMCC No. 14393, preservation date
For：On July 7th, 2017, preservation address are：BeiJing, China.
The present inventor is by the way that from being to screen to obtain in the organic fertilizer that raw material ferment with chicken manure etc., isolation medium uses
The continuous enrichment culture of carbon-free culture medium using tetracycline as sole carbon source, flat board isolate and purify to obtain highly resistance to tetracycline
Single bacterium colony, isolated single bacterium colony sterile working is added in the nutrient solution containing finite concentration tetracycline and cultivated, and set
Blank control group is tested, and the concentration that tetracycline is remained in nutrient solution, each bacterium of com-parison and analysis are determined with HPLC after cultivating certain time
Fall the degradation efficiency to tetracycline, further filter out tetracycline efficient degrading bacterial strain.The 16SrDNA gene orders of the bacterial strain
See SEQ ID No1.By physio-biochemical characteristics and 16SrDNA sequence analyses, the bacterial strain is accredited as Klebsiella
(Klebsiellapneumoniae), and it is named as TR5.
Application of the above-mentioned tetracycline efficient degradation bacterium in culture fishery water body purification.
Application of the above-mentioned tetracycline efficient degradation bacterium in the waste water control of tetracycline antibiotics pharmaceutical factory.
Beneficial effect：The present invention will be the industrialization of tetracycline efficient degrading bacteria by microbial immobilized processing method
Using providing theoretical foundation, presence that the immobilized spherules of TR5 bacterial strains of the invention can be stable in water body, in water body
Fourth Ring is known as good degradation effect, and can be recycled, and has a higher biological safety, and application cost is low and not
Secondary pollution can be triggered, culture fishery water body purification is can be applied to and tetracycline antibiotics pharmacy sewage is administered.
Brief description of the drawings
Fig. 1 is TR5 evolution tree graph；
Fig. 2 is different carbon source to TR5 upgrowth situations and the influence figure of degradation efficiency；
Fig. 3 is different carbon source and influence of the different nitrogen sources compatibility to TR5 upgrowth situations and degradation efficiency；
Fig. 4 is temperature to TR5 upgrowth situations and the influence figure of degradation efficiency；
Fig. 5 is rotating speed to TR5 upgrowth situations and the influence figure of degradation efficiency
Fig. 6 is pH to TR5 upgrowth situations and the influence figure of degradation efficiency；
Fig. 7 is inoculum concentration to TR5 upgrowth situations and the influence figure of degradation efficiency.
Embodiment
Embodiment 1
Separation, purifying and the preservation of bacterial strain
Weigh the liquid for the organic manure 5g that raw material ferment to 50ml gradients containing various concentrations tetracycline with chicken manure etc.
Culture medium, and using tetracycline as sole carbon source (culture medium prescription as：Contain 5g (NH4) in per 1L nutrient solutions2SO4、1g
KH2PO4、0.5g MgSO4·7H2O、0.1g CaCl2, 0.1g NaCl), 7d is cultivated under 28 DEG C, 150rpm, according to same
Condition of culture by continuous enrichment culture, obtain what can be grown under conditions of using high concentration tetracycline as sole carbon source
Bacterial strain, the bacteria suspension of following absorption 0.2% are coated on the solid culture containing higher concentration tetracycline and using it as carbon source
Base, typically single bacterium colony carries out isolation and purification culture to picking after cultivating 2~3d, the pure culture bacterial strain that final separation is obtained
High concentration tetracycline nutrient solution culture is carried out, the initial concentration of tetracycline in nutrient solution, and the timing of culture one are measured using HPLC
Between after in nutrient solution tetracycline residual concentration, obtain tetracycline degradation efficiency corresponding to different pure culture bacterial strains, analyze ratio
Compared with data, tetracycline efficient degrading bacteria is obtained, the 16SrDNA gene orders of the bacterial strain are shown in SEQ ID No1, pass through Physiology and biochemistry
Characteristic and 16SrDNA sequence analyses, the bacterial strain are accredited as Klebsiella (Klebsiellapneumoniae), and will
It is named as TR5.The bacterial strain is preserved in China Microbial Culture Preservation Commission's common micro-organisms on July 7th, 2017
The heart, deposit number are CGMCC No. 14393.
Embodiment 2
The influence of growth and degrade tetracycline of different carbon source, nitrogen source, pH, temperature and the inoculum concentrations to TR5
Carbon source needed for experimental group using the carbohydrates as strain growth such as glucose, starch, lactose, with yeast extract, albumen
Peptone, casein etc. are as the nitrogen source needed for strain growth, pH 3~11, temperature at 15 DEG C~40 DEG C, inoculum concentration is 2%~
Tested under the conditions of 10% laboratory cultures.
Fig. 1 is TR5 evolution tree graph.
Fig. 2 be carbon source to TR5 upgrowth situations and the influence figure of degradation efficiency, as seen from Figure 2, because sodium acetate is in
Alkalescence, and the unstable easily decomposition of tetracycline under alkalescence condition, upgrowth situation of the TR5 bacterial strains under this laboratory condition is also bad,
Finally determined from glucose as the optimum carbon source under the bacterial strain laboratory condition according to this figure.
Fig. 3 is different carbon source and different nitrogen sources compatibility to TR5 upgrowth situations and the influence figure of degradation efficiency；Can be with by Fig. 3
Find out, using glucose as optimum carbon source, while using yeast extract as nitrogen source in the case of, TR5 to tetracycline degraded effect
Rate is optimal, therefore final choice yeast extract is as the optimum nitrogen source under the bacterial strain laboratory condition.
Fig. 4 be temperature to TR5 upgrowth situations and the influence figure of degradation efficiency, as seen from Figure 4, TR5 bacterial strains are in 25-
Tetracycline efficiency of being degraded under the conditions of 30 DEG C is higher.
Fig. 5 be rotating speed to TR5 upgrowth situations and the influence figure of degradation efficiency, as seen from Figure 5, TR5 bacterial strains are in rotating speed
For 180rpm when degradation efficiency it is optimal, according to growth curve can initial guess, the bacterium is more aerobic.
Fig. 6 be pH to TR5 upgrowth situations and the influence figure of degradation efficiency, as seen from Figure 6, TR5 is in pH7.0 or so
It is higher to the degradation efficiency of tetracycline.
Fig. 7 be inoculum concentration to TR5 upgrowth situations and the influence figure of degradation efficiency, as seen from Figure 7, inoculum concentration is to four
The degraded of ring element simultaneously has no significant effect.
To sum up, experimental result shows the bacterial strain using glucose as carbon source, and yeast extract is as nitrogen source, pH 7~9,
Temperature degradation efficiency at 25 DEG C~30 DEG C is higher, and inoculum concentration influences little on degradation efficiency.
Embodiment 3
Degradation capability determination experiment
1st, application of the tetracycline efficient degradation bacterium (TR5) in culture fishery water body purification
The water in certain artificial farming fish pond is taken, after water body is carried out into preliminary purification processing, with HPLC to tetracycline in water body
Content is measured, and is 200ppm, the moisture in fish pond is attached into blake bottle (every bottle of 20ml), and add 20 in every bottle of culture
The beads of immobilization embedded TR5 bacterial strains cultivated, and a blank control group is set, cultivated after 5d to blake bottle
The content of middle tetracycline is determined again, finds experimental group compared with control group, and Fourth Ring cellulose content has obvious reduction,
Experimental group degradation efficiency is 86%.
2nd, application of the tetracycline efficient degradation bacterium in the waste water control of tetracycline antibiotics pharmaceutical factory
The tetracycline antibiotics pharmacy waste water for taking certain pharmaceutical factory to discharge, by waste water after preliminary purification is handled, reference
State's calibration method is measured with HPLC to the content of tetracycline in water body, is 435ppm, by pharmacy waste water quantitative separating to training
Bottle (every bottle of 20ml) is supported, and the bead for adding in every bottle of culture the immobilization embedded TR5 bacterial strains of 20 is cultivated, and
One blank control group is set, the content of tetracycline in blake bottle determined again after cultivating 5d, discovery experimental group with it is right
Compared according to group, Fourth Ring cellulose content has obvious reduction, and experimental group degradation efficiency is 88%.
Claims (3)
1. a kind of tetracycline efficient degradation bacterium, it is characterised in that the bacterium is Klebsiella, and it is micro- to be preserved in China
Biological inoculum preservation committee common micro-organisms center, deposit number are CGMCC No.14393, and preservation date is：2017 7
The moon 7, preservation address are：BeiJing, China.
2. application of the tetracycline efficient degradation bacterium in culture fishery water body purification described in claim 1.
3. application of the tetracycline efficient degradation bacterium described in claim 1 in the waste water control of antibiotic pharmaceutical factory.
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| CN201711212339.8A CN107858305B (en) | 2017-11-28 | 2017-11-28 | Bacterium for efficiently degrading tetracycline and application thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108707559A (en) * | 2018-05-15 | 2018-10-26 | 浙江省农业科学院 | A kind of tetracycline antibiotics degradation arthrobacterium and its application |
| CN109337825A (en) * | 2018-11-09 | 2019-02-15 | 北京农学院 | One plant of Beijing Paecilomyces varioti bacterial strain LYZ7 and its application |
| CN110592067A (en) * | 2019-09-20 | 2019-12-20 | 广东省农业科学院农业资源与环境研究所 | In-situ microorganism repairing agent for tetracycline antibiotic contaminated soil, preparation method and application |
| CN113832117A (en) * | 2021-09-23 | 2021-12-24 | 盐城师范学院 | Enzyme for degrading oxytetracycline, and coding gene and application thereof |
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| CN105110486A (en) * | 2015-09-01 | 2015-12-02 | 哈尔滨工业大学 | Method for removing tetracycline from water by virtue of microbial flocculant |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108707559A (en) * | 2018-05-15 | 2018-10-26 | 浙江省农业科学院 | A kind of tetracycline antibiotics degradation arthrobacterium and its application |
| CN109337825A (en) * | 2018-11-09 | 2019-02-15 | 北京农学院 | One plant of Beijing Paecilomyces varioti bacterial strain LYZ7 and its application |
| CN109337825B (en) * | 2018-11-09 | 2021-06-15 | 北京农学院 | Paecilomyces beijing strain LYZ7 and application thereof |
| CN110592067A (en) * | 2019-09-20 | 2019-12-20 | 广东省农业科学院农业资源与环境研究所 | In-situ microorganism repairing agent for tetracycline antibiotic contaminated soil, preparation method and application |
| CN113832117A (en) * | 2021-09-23 | 2021-12-24 | 盐城师范学院 | Enzyme for degrading oxytetracycline, and coding gene and application thereof |
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