CN113832117A - Enzyme for degrading oxytetracycline, and coding gene and application thereof - Google Patents
Enzyme for degrading oxytetracycline, and coding gene and application thereof Download PDFInfo
- Publication number
- CN113832117A CN113832117A CN202111115813.1A CN202111115813A CN113832117A CN 113832117 A CN113832117 A CN 113832117A CN 202111115813 A CN202111115813 A CN 202111115813A CN 113832117 A CN113832117 A CN 113832117A
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- China
- Prior art keywords
- enzyme
- degrading
- tetracycline antibiotic
- seq
- gly
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- 108090000790 Enzymes Proteins 0.000 title claims abstract description 49
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 49
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 33
- 230000000593 degrading effect Effects 0.000 title claims abstract description 25
- 239000004100 Oxytetracycline Substances 0.000 title claims abstract description 13
- 229960000625 oxytetracycline Drugs 0.000 title claims abstract description 13
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- 235000019366 oxytetracycline Nutrition 0.000 title claims abstract description 13
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- 229940072172 tetracycline antibiotic Drugs 0.000 claims abstract description 24
- 238000002360 preparation method Methods 0.000 claims abstract description 15
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- 238000004140 cleaning Methods 0.000 claims abstract description 5
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- 238000000034 method Methods 0.000 claims description 11
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- 230000015556 catabolic process Effects 0.000 abstract description 13
- 238000006731 degradation reaction Methods 0.000 abstract description 13
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- 238000010353 genetic engineering Methods 0.000 abstract description 2
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Abstract
The invention provides an enzyme for degrading oxytetracycline, and a coding gene and application thereof, belonging to the technical field of biological engineering. Derived from Klebsiella with the preservation number of CGMCC No.14393, the amino acid sequence is as follows: shown in SEQ ID No. 2. The invention adopts a genetic engineering method to carry out induction expression on the coding gene in escherichia coli BL21(DE3), after the fermentation liquor is centrifuged, strains are taken for cleaning, ultrasonic crushing is carried out, and Histrap1mL affinity column is adopted for purification to obtain enzyme liquor; the enzyme liquid (200U) is used for treating an oxytetracycline sample (the initial concentration is 20mmol/L), and the degradation efficiency can reach 75.3%. The enzyme preparation has the characteristics of low cost, short period, simple and convenient operation and the like, and is expected to be applied to biodegradation of tetracycline antibiotics in the environment.
Description
Technical Field
The invention belongs to the technical field of biological engineering, and particularly relates to an enzyme for degrading oxytetracycline, and a coding gene and application thereof.
Background
The tetracycline antibiotics can inhibit intestinal bacteria reproduction and promote livestock growth, and are widely used feed additives. However, due to lack of perfect supervision, the abuse of tetracycline antibiotics in China is serious, and the usage amount in 2013 is 12000 tons. Tetracycline antibiotics are not completely absorbed by animals, and part of the tetracycline antibiotics are excreted with feces. If the tetracycline is directly applied to farmlands without harmless treatment, residual tetracycline can be accumulated in soil. When the continuous antibiotic selection pressure gene in human and animal bodies is activated after rainwater enters a river system and then enters a human body through a food chain, the propagation risk of drug resistance is increased, and thus, the potential threat to the health safety of human is formed. Therefore, how to degrade tetracycline antibiotics in the environment has become a focus issue. The microbial degradation is a method with the lowest cost and wide application prospect, and the essence of the method is the degradation of tetracycline antibiotics by enzyme preparations. The invention provides an enzyme preparation which is produced in a microorganism body and can degrade tetracycline antibiotics.
Disclosure of Invention
The invention aims to provide an enzyme for degrading oxytetracycline, a coding gene and application thereof aiming at the defects in the prior art, wherein the enzyme has definite degrading gene, higher safety and excellent effect on degrading tetracycline antibiotics in the environment.
The invention aims to provide a tetracycline antibiotic degrading enzyme, which is derived from Klebsiella sp.TR5 with the preservation number of CGMCC No.14393, and the amino acid sequence of the degrading enzyme is as follows: shown in SEQ ID No. 2.
The second purpose of the invention is to provide a gene for coding the tetracycline antibiotic degrading enzyme, and the nucleotide sequence of the gene is as follows: SEQ ID No. 1.
The third purpose of the invention is to provide a method for expressing the tetracycline antibiotic degrading enzyme, which comprises the following steps:
extracting plasmid DNA from Klebsiella bacterial liquid as a template, and amplifying target genes by using a primer 1 represented by SEQ ID No.3 and a primer 2 represented by SEQ ID No. 4;
and (2) connecting the target gene into a pET28(a +) expression vector, then transforming the target gene into a host cell BL21(DE3), fermenting and recombining the host cell to express the target gene, and thus obtaining the tetracycline antibiotic degrading enzyme.
Preferably, primer 1 and primer 2 have restriction sites for EcoRI and XhoI enzymes, respectively.
Preferably, in the process of fermenting the recombinant host cell, 0.025mmol/L IPTG is used for inducing expression for 12 h; centrifuging the fermentation liquor, taking the strain, cleaning, carrying out ultrasonic crushing, and purifying by using a Histrap1mL affinity column.
The fourth purpose of the invention is to provide an enzyme preparation containing the tetracycline antibiotic-degrading enzyme.
The fifth purpose of the invention is to provide the application of the enzyme preparation in degrading tetracycline antibiotics.
Preferably, the enzyme preparation is used for degrading oxytetracycline.
Compared with the prior art, the invention has the beneficial effects that:
compared with the degradation of tetracycline by Klebsiella, the tetracycline antibiotic degrading enzyme provided by the invention has definite degrading gene and higher safety, and has excellent effect on the degradation of tetracycline antibiotic in the environment.
The gene coding the enzyme preparation in the Klebsiella is expressed in Escherichia coli E.coli BL21(DE3) by adopting a genetic engineering method: specifically, the target gene is amplified by a primer, EcoRI and XhoI are used for double enzyme digestion, and are connected with pET28(a +), the vector is introduced into BL21(DE3) competent cells, and the expression is induced by 0.025mmol/L IPTG for 12 h. And centrifuging the fermentation liquor, taking the strain, cleaning, carrying out ultrasonic crushing, purifying by using a Histrap1mL affinity column to obtain enzyme liquid, adding the enzyme liquid into a sample containing oxytetracycline with the concentration of 20mmol/L according to the concentration of 200U for degradation, and setting the degradation efficiency of a control group (engineering bacteria fermentation liquor containing empty carriers) to be 75.3%. The enzyme preparation has the characteristics of low cost, short period, simple and convenient operation and the like, and is expected to be applied to biodegradation of tetracycline antibiotics in the environment.
Drawings
FIG. 1 is a SDS-PAGE pattern of purification of an enzyme preparation provided by the present invention.
Detailed Description
The present invention is described in detail below with reference to the drawings and the specific embodiments, but it should be understood that the scope of the present invention is not limited by the specific embodiments. The test methods in the following examples, which are not specified in specific conditions, are generally conducted under conventional conditions, and the steps thereof will not be described in detail since they do not relate to the invention.
When numerical ranges are given in the examples, it is understood that both endpoints of each of the numerical ranges and any value therebetween can be selected unless the invention otherwise indicated. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In addition to the specific methods, devices, and materials used in the examples, any methods, devices, and materials similar or equivalent to those described in the examples may be used in the practice of the invention in addition to the specific methods, devices, and materials used in the examples, in keeping with the knowledge of one skilled in the art and with the description of the invention.
The Klebsiella sp.TR5 adopted by the invention is preserved in the China general microbiological culture Collection center in 7 months and 7 days in 2017, the preservation number is CGMCC No.14393, and the preservation address is as follows: beijing, China; the Klebsiella has been disclosed in Chinese patent No. CN 201711212339.8.
The primer 1 and the primer 2 are from Wuxi sanderie Biotechnology GmbH.
Example 1
A method for expressing tetracycline antibiotic degrading enzyme comprises the following steps:
a single colony of Klebsiella was picked, inoculated into a 30mL Erlenmeyer flask containing LB medium, and cultured overnight with shaking at 37 ℃. Extracting plasmid DNA from the bacterial liquid by adopting a plasmid extraction kit according to the specification of the plasmid extraction kit, and amplifying a target gene under the guidance of a primer 1 represented by SEQ ID No.3 and a primer 2 represented by SEQ ID No. 4;
wherein,
primer 1: 5' -CCGGAATTCATGAAATCACGCGCAGCAGT-3' (EcoRI cleavage site is underlined, and the gene sequence is SEQ ID No.3),
primer 2: 5' -CGGCTCGAGGTAATGAATGACTGTGCGGATG-3' (XhoI restriction site underlined, gene sequence SEQ ID No. 4);
the target gene is amplified from the plasmid DNA, after the target gene is obtained, the target gene is sequenced, and the sequencing result shows that the gene has the nucleotide sequence of SEQ ID No.1 in the sequence table, and the corresponding amino acid sequence is SEQ ID No. 2.
Then carrying out double enzyme digestion on the target gene by utilizing EcoRI and XhoI, connecting the target gene into a pET28(a +) expression vector, transforming the vector into a host cell BL21(DE3), and carrying out induced expression for 12h by utilizing 0.025mmol/L IPTG; and centrifuging the fermentation liquor, taking the strain, cleaning, crushing by ultrasonic waves, and purifying by using a Histrap1mL affinity column to obtain enzyme liquid, namely the tetracycline antibiotic degrading enzyme.
In order to illustrate the relevant performance of the tetracycline antibiotic degrading enzyme provided by the present invention, the relevant samples provided in example 1 were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) test; FIG. 1 shows, wherein lane 0 is blank in FIG. 1; 1 is control; 2, crushing liquid supernatant; 3 is a sample after affinity chromatography separation; as can be seen from FIG. 1, the target gene was successfully expressed in E.coli, and its molecular weight was about 3.9 kDa.
Example 2
A tetracycline antibiotic-degrading enzyme formulation comprising the tetracycline antibiotic-degrading enzyme provided in example 1; the enzyme preparation was prepared by dissolving the tetracycline antibiotic-degrading enzyme provided in example 1 in Tris-HCl pH 7.5.
In order to illustrate the degradation performance of the tetracycline antibiotic degradation enzyme preparation provided by the present invention, the enzyme preparation provided in example 2 was added to a sample containing oxytetracycline at a concentration of 20mmol/L at a concentration of 200U for degradation, and the degradation rate at different degradation durations was monitored, as shown in table 1, wherein the control group was the engineering bacteria fermentation broth containing empty vector.
Table 1 degradation rates of oxytetracycline by enzyme formulations provided in example 2
| 0h | 6h | 12h | | 24h | |
| Control | |||||
| 0 | 0.5％ | 1.1％ | 1.8％ | 2.2％ | |
| Experiment of | 0 | 25.2％ | 48.5％ | 64.1％ | 75.3％ |
As can be seen from Table 1, the enzyme preparation provided by the invention has the highest efficiency of degrading oxytetracycline of 75.3%.
While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Sequence listing
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<120> enzyme for degrading oxytetracycline, and coding gene and application thereof
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cataccgatg cctacacgct ttccggtgat gatccggaag gtctgttccc ggtggtgctc 180
ggccatgaag gtgccggggt ggtggttgag gtcggtgagg gcgtgaccag tgtgaaaccg 240
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gcggagcagg tttgcctgct cggctgcggc gtgaccaccg ggatcggcgc ggtacacaat 540
accgcgaagg tacaggaagg ggattctgtg gcggtattcg gcctcggcgg tatcgggctg 600
gccgtggtgc agggcgcacg tcaggcaaaa gcggggcgca tctttgccat cgataccaat 660
ccatccaagt ttgagctggc aaaacagttc ggcgccacag actgcattaa tcccaatgat 720
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tttgaatgta tcggcaatgt caatgtgatg cggtcggcgc tggaaagtgc gcaccgtggc 840
tgggggcagt cggtaattat cggggtggcg ggtgccggaa aagagatttc aacccgtccg 900
ttccagctgg tgaccggccg ggtctggaaa ggcacggcgt tcggcggcgt gaaagggcgt 960
acccagttgc cgggaatggt ggaagatgcc atgagcggca aaatcgagct ggcgccgttt 1020
gtcacgcaca ccatggaact ggataaaatc aatgaagctt ttgatttgat gcacgacggc 1080
aaatccatcc gcacagtcat tcattactga 1110
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Claims (8)
1. The tetracycline antibiotic degrading enzyme is derived from Klebsiella sp.TR5 with the preservation number of CGMCC No.14393, and has the amino acid sequence as follows: shown in SEQ ID No. 2.
2. A gene encoding the tetracycline antibiotic-degrading enzyme of claim 1 having the nucleotide sequence: SEQ ID No. 1.
3. A method for expressing the tetracycline antibiotic-degrading enzyme of claim 1, comprising the steps of:
extracting plasmid DNA from Klebsiella bacterial liquid as a template, and amplifying target genes by using a primer 1 represented by SEQ ID No.3 and a primer 2 represented by SEQ ID No. 4;
and (2) connecting the target gene into a pET28(a +) expression vector, then transforming the target gene into a host cell BL21(DE3), fermenting and recombining the host cell to express the target gene, and thus obtaining the tetracycline antibiotic degrading enzyme.
4. The method for expressing a tetracycline antibiotic-degrading enzyme of claim 3, wherein primer 1 and primer 2 have EcoRI and XhoI cleavage sites, respectively.
5. The method for expressing tetracycline antibiotic-degrading enzyme of claim 3, wherein in fermenting the recombinant host cell, expression is induced with 0.025mmol/L IPTG for 12 h; centrifuging the fermentation liquor, taking the strain, cleaning, carrying out ultrasonic crushing, and purifying by using a Histrap1mL affinity column.
6. An enzyme preparation comprising the tetracycline antibiotic-degrading enzyme of claim 1.
7. Use of the enzyme preparation of claim 6 for degrading a tetracycline antibiotic.
8. Use according to claim 7, wherein the enzyme preparation is for degrading oxytetracycline.
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| CN202111115813.1A CN113832117B (en) | 2021-09-23 | 2021-09-23 | Enzyme for degrading oxytetracycline, and coding gene and application thereof |
| ZA2022/05132A ZA202205132B (en) | 2021-09-23 | 2022-05-10 | Oxytetracycline-degrading enzyme, and encoding gene and use thereof |
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|---|---|---|---|---|
| CN115851630A (en) * | 2022-12-06 | 2023-03-28 | 盐城师范学院 | Tetracycline antibiotic degrading enzyme, encoding gene and application |
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| US6136605A (en) * | 1994-08-26 | 2000-10-24 | Wisconsin Alumni Research Foundation | Glutathione S-transferase isoforms |
| CN107858305A (en) * | 2017-11-28 | 2018-03-30 | 盐城师范学院 | One plant of tetracycline efficient degradation bacterium and its application |
| CN110042111A (en) * | 2019-04-12 | 2019-07-23 | 华南农业大学 | The gene and its albumen of a kind of tetracycline antibiotics that can degrade and application |
| CN110305882A (en) * | 2019-06-21 | 2019-10-08 | 广东实验中学 | A kind of zymoprotein of the gene and its coding of tetracycline antibiotics of degrading |
| CN111662839A (en) * | 2020-06-01 | 2020-09-15 | 华南农业大学 | Bacillus belgii for degrading tetracycline, magnetic immobilized microorganism composite material and application |
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2021
- 2021-09-23 CN CN202111115813.1A patent/CN113832117B/en active Active
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| US6136605A (en) * | 1994-08-26 | 2000-10-24 | Wisconsin Alumni Research Foundation | Glutathione S-transferase isoforms |
| CN107858305A (en) * | 2017-11-28 | 2018-03-30 | 盐城师范学院 | One plant of tetracycline efficient degradation bacterium and its application |
| CN110042111A (en) * | 2019-04-12 | 2019-07-23 | 华南农业大学 | The gene and its albumen of a kind of tetracycline antibiotics that can degrade and application |
| CN110305882A (en) * | 2019-06-21 | 2019-10-08 | 广东实验中学 | A kind of zymoprotein of the gene and its coding of tetracycline antibiotics of degrading |
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| CN115851630A (en) * | 2022-12-06 | 2023-03-28 | 盐城师范学院 | Tetracycline antibiotic degrading enzyme, encoding gene and application |
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