CN107324609A - It is a kind of to improve the method for culture environment of aquatic products - Google Patents
It is a kind of to improve the method for culture environment of aquatic products Download PDFInfo
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- CN107324609A CN107324609A CN201710740084.6A CN201710740084A CN107324609A CN 107324609 A CN107324609 A CN 107324609A CN 201710740084 A CN201710740084 A CN 201710740084A CN 107324609 A CN107324609 A CN 107324609A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Abstract
Improve the method for culture environment of aquatic products the invention discloses a kind of, it is main to include three steps, cleaning pond bottom simultaneously carries out sterilization processing using electrochemical water, delivers complex microorganism and composite reactive enzyme after pond bottom lays bottom of pond material and sprays expanded vermiculite suspension, water filling, three steps are indispensable, it can not overturn, it is step by step progressive, it is all linked with one another, so as to improve culture environment of aquatic products.
Description
Technical field
Improve the method for culture environment of aquatic products the present invention relates to a kind of.Belong to technical field of aquaculture.
Background technology
The culture fishery development of China is swift and violent, is the fishery big country that unique cultured output exceedes fishery output in the world.
At present, domestic aquaculture is based on traditional aquaculture model, it is characterized in that " close, mixed, wheel ", be with multi items, high density and
Heavy dressing bait throwing in and the backwardness aquaculture model for obtaining high yield.In order to improve the cultured output of unit of water body, covet height
Production, often undue increase water nutrition, excessive bait throwing in, fertilising and increase breeding density, cause nitrogen and phosphorus content in water to surge, thoroughly
Lightness declines, water quality deterioration, and the metabolin of bait and breed variety in water body largely accumulates in pond bottom, seriously polluted.
If things go on like this, the natural, ecological of water body is destroyed, and breeding spaces aquatic animal disease spreads, and aquatic animal disease frequently occurs,
Fishery loss is huge.
In order to prevent disease, the situation of fish medicine is used in the current generally existing whole culture-cycle, but fish medicine is using only
It can solve for the moment anxious, more bring the resistance to the action of a drug and the ecological consequence, shape such as accumulation residual in aquatic products and environment
Into vicious circle, there is no the improvement of essence.
The content of the invention
The purpose of the present invention is that there is provided a kind of side of improvement culture environment of aquatic products to overcome above-mentioned the deficiencies in the prior art
Method.
To achieve the above object, the present invention uses following technical proposals：
It is a kind of to improve the method for culture environment of aquatic products, comprise the following steps：
（1）Water in emptying pond, clears up pond bottom, introduces clean water to 5~8cm of the depth of water, electrochemical water of splashing is sterilized
Handle, again the water in emptying pond；
（2）One layer of bottom of pond material is uniformly laid in pond bottom, one layer of expanded vermiculite suspension is then uniformly sprayed；
（3）Clean water is slowly injected into more than depth of water 1.5m, complex microorganism and composite reactive enzyme are then delivered into pond, i.e.,
Can.
It is preferred that, step（1）Electrochemical water be using electro-chemical water processing equipment mass concentration is not less than 2 ‰ chlorination
Sodium solution is prepared, and indices are as follows：Effective chlorine mass concentration is 70~100mg/L, and ozone 3~4mg/L, pH are 6.5
~7.5, oxidation-reduction potential is 900~1100mV, 15~20mg/L of hydrogen peroxide.
It is preferred that, step（1）In, cleaning pond bottom includes：The mud and debris of pond bottom are removed, and uses clean water
Wash away 3~4 times.
It is preferred that, step（1）The amount of splashing of middle electrochemical water is 2~5% of water weight in pond.
It is preferred that, step（1）Middle electrochemical treatment time of water is 3~4 hours.
It is preferred that, step（2）The laying depth of middle bottom of pond material is 8~12cm.
It is preferred that, step（2）In, the bottom of pond material is mixed by the component of following parts by weight：River sand 100
Part, 30~50 parts of flyash, 10~20 parts of fertile soil, 10~20 parts of bentonite.
It is preferred that, step（2）In, the fountain height of expanded vermiculite suspension is 2~3L/m2。
It is preferred that, step（2）Described in expanded vermiculite suspension be prepared by the following method and obtain：
（21）Crude vermiculite is taken to be heated 200~250 minutes in 400~500 DEG C, natural cooling obtains expanded vermiculite；
（22）Sodium humate is soluble in water, poly aluminium iron silicate and EDTA chelated coppers are then sequentially added, is stirred and evenly mixed, is sought
Support agent；
（23）By step（21）Gained expanded vermiculite puts into step（22）Soak 18~24 hours, expanded in gained nutritional agents
Vermiculite suspension.
It is further preferred that step（22）In, sodium humate, poly aluminium iron silicate, the quality volume of EDTA chelated coppers and water
Than for 8~10g：2~3g：0.5~0.6g：100mL.
It is preferred that, step（3）Using sprinkling injection during middle injection clean water, it is 5~6 hours to control injection length.
It is preferred that, step（3）In, the injected volume of complex microorganism and composite reactive enzyme is respectively 1 of water weight in pond
~2% and 3~4%.
It is preferred that, the preparation method of complex microorganism is as follows：By photosynthetic bacteria, colloid bacillus cereus, trichoderma harzianum list
The zymotic fluid of each strain is solely respectively obtained after fermentation, then mixed fermentation is produced.
It is further preferred that photosynthetic bacteria in complex microorganism, colloid bacillus cereus, trichoderma harzianum strain content according to
Secondary is 40~5,000,000,000/g, 20~3,000,000,000/g, 15~2,000,000,000/g.
It is further preferred that individually fermentation or the culture medium used during mixed fermentation be selected from beef-protein medium,
Any of potato culture or LB culture mediums.
Still more preferably, fermentation temperature is 46~48 DEG C, and fermentation time is 20~24 hours.
It is preferred that, the preparation method of composite reactive enzyme is as follows：By neutral proteinase, amylase, lipase and cellulase
According to mass ratio 1：1：1：1 is well mixed, and the mass ratio 1 of 3~4 times of gross weights is then admixed in the lump：1 mix montmorillonite with it is convex
In Attapulgite mixture.
It is further preferred that neutral proteinase, amylase, the enzyme activity of lipase and cellulase be followed successively by 120,000 U/g,
5000U/g, 10,000 U/g and 120,000 U/g.
Beneficial effects of the present invention：
1st, the invention mainly comprises three steps, clear up pond bottom and carry out sterilization processing, in pond bottom using electrochemical water
Laying bottom of pond material simultaneously sprays dispensing complex microorganism and composite reactive enzyme after expanded vermiculite suspension, water filling, and three steps lack
One can not, can not overturn, it is step by step progressive, it is all linked with one another, so as to improve culture environment of aquatic products.
2nd, the water in emptying pond, clears up pond bottom, and the deterioration dirt that long-time can be deposited on into pond bottom is clear
Remove, shallower clean water is introduced on this basis, can be to sticking to the remaining bacteria of pond bottom with reference to sprinkling electrochemical water
Deng being killed, so as to realize the thorough cleaning of pond bottom；Then lay new bottom of pond material in pond bottom and spray swollen
Swollen vermiculite suspension, can build new pool bottom environment, expanded vermiculite suspension has adsorbed nutritional agents, due to expanded vermiculite
With loose structure, similar sustained release is played a part of to the nutritional agents in it, nutritional agents can be slowly discharged for a long time, promotes water
Ecological environment in grass growth, optimization pond, treats the release of nutritional agents part or release completely, and the loose structure of expanded vermiculite is returned respectively
Plant microorganism and environment of living away from home is provided, further promote the formation of microbial ecological in pond；Clean water is finally slowly injected into, can be kept away
Exempt from clean water and be rapidly injected to cause the erosion damage to bottom of pond material, deliver complex microorganism and composite reactive enzyme, contribute to net
Change water body and antibacterial etc., further improve culture environment of aquatic products.
3rd, bottom of pond material of the invention is made up of river sand, flyash, fertile soil and bentonite, with preferably gas permeability,
Growing for pond bottom pathogenic microorganism can be avoided（Pond is largely grown using the microorganism put into initial stage, also can extrude even
Break off the growth space of pathogenic microorganism）, and be conducive to the expansion of pasture and water root system, improve pond bottom living environment.
Embodiment
With reference to embodiment, the present invention will be further elaborated, it should explanation, the description below merely to
The present invention is explained, its content is not defined.
Embodiment 1：
It is a kind of to improve the method for culture environment of aquatic products, comprise the following steps：
（1）Water in emptying pond, clears up pond bottom, introduces clean water to depth of water 5cm, electrochemical water of splashing is carried out at sterilization
Manage, again the water in emptying pond；
（2）One layer of bottom of pond material is uniformly laid in pond bottom, one layer of expanded vermiculite suspension is then uniformly sprayed；
（3）Clean water is slowly injected into more than depth of water 1.5m, complex microorganism and composite reactive enzyme are then delivered into pond, i.e.,
Can.
Wherein, step（1）Electrochemical water be using electro-chemical water processing equipment mass concentration is not less than 2 ‰ sodium chloride
Solution is prepared, and indices are as follows：Effective chlorine mass concentration is 70mg/L, and ozone 3mg/L, pH are 6.5, redox electricity
Position is 900mV, hydrogen peroxide 15mg/L.
Cleaning pond bottom includes：The mud and debris of pond bottom are removed, and is washed away 3 times with clean water.
The amount of splashing of electrochemical water is 2% of water weight in pond.Electrochemical treatment time of water is 3 hours.
Step（2）The laying depth of middle bottom of pond material is 8cm.
Bottom of pond material is mixed by the component of following parts by weight：100 parts of river sand, 30 parts of flyash, fertile soil 10
Part, 10 parts of bentonite.
The fountain height of expanded vermiculite suspension is 2L/m2.Expanded vermiculite suspension, which is prepared by the following method, to be obtained：
（21）Crude vermiculite is taken to be heated 200 minutes in 400 DEG C, natural cooling obtains expanded vermiculite；
（22）Sodium humate is soluble in water, poly aluminium iron silicate and EDTA chelated coppers are then sequentially added, is stirred and evenly mixed, is sought
Support agent；
（23）By step（21）Gained expanded vermiculite puts into step（22）Soaked 18 hours in gained nutritional agents, obtain expanded vermiculite
Suspension.
Step（22）In, sodium humate, poly aluminium iron silicate, the mass volume ratio of EDTA chelated coppers and water are 8g：2g：
0.5g：100mL.
Step（3）Using sprinkling injection during middle injection clean water, it is 5 hours to control injection length.
The injected volume of complex microorganism and composite reactive enzyme is respectively 1% and 3% of water weight in pond.
The preparation method of complex microorganism is as follows：After photosynthetic bacteria, colloid bacillus cereus, trichoderma harzianum individually fermentation
The zymotic fluid of each strain is respectively obtained, then mixed fermentation is produced.Photosynthetic bacteria, colloid bacillus cereus, Kazakhstan in complex microorganism
The strain content of thatch Trichoderma is followed successively by 4,000,000,000/g, 2,000,000,000/g, 1,500,000,000/g.The culture medium used when independent fermentation or mixed fermentation
For beef-protein medium.Fermentation temperature is 46 DEG C, and fermentation time is 20 hours.
The preparation method of composite reactive enzyme is as follows：By neutral proteinase, amylase, lipase and cellulase according to quality
Than 1：1：1：1 is well mixed, and the mass ratio 1 of 3 times of gross weights is then admixed in the lump：1 montmorillonite mixed is mixed with concavo-convex rod soil
In thing.Neutral proteinase, amylase, the enzyme activity of lipase and cellulase be followed successively by 120,000 U/g, 5000U/g, 10,000 U/g and
120000 U/g.
Embodiment 2：
It is a kind of to improve the method for culture environment of aquatic products, comprise the following steps：
（1）Water in emptying pond, clears up pond bottom, introduces clean water to depth of water 8cm, electrochemical water of splashing is carried out at sterilization
Manage, again the water in emptying pond；
（2）One layer of bottom of pond material is uniformly laid in pond bottom, one layer of expanded vermiculite suspension is then uniformly sprayed；
（3）Clean water is slowly injected into more than depth of water 1.5m, complex microorganism and composite reactive enzyme are then delivered into pond, i.e.,
Can.
Wherein, step（1）Electrochemical water be using electro-chemical water processing equipment mass concentration is not less than 2 ‰ sodium chloride
Solution is prepared, and indices are as follows：Effective chlorine mass concentration is 100mg/L, and ozone 4mg/L, pH are 7.5, redox
Current potential is 1100mV, hydrogen peroxide 20mg/L.
Cleaning pond bottom includes：The mud and debris of pond bottom are removed, and is washed away 4 times with clean water.
The amount of splashing of electrochemical water is 5% of water weight in pond.Electrochemical treatment time of water is 4 hours.
Step（2）The laying depth of middle bottom of pond material is 12cm.
Bottom of pond material is mixed by the component of following parts by weight：100 parts of river sand, 50 parts of flyash, fertile soil 20
Part, 20 parts of bentonite.
The fountain height of expanded vermiculite suspension is 3L/m2.Expanded vermiculite suspension, which is prepared by the following method, to be obtained：
（21）Crude vermiculite is taken to be heated 250 minutes in 500 DEG C, natural cooling obtains expanded vermiculite；
（22）Sodium humate is soluble in water, poly aluminium iron silicate and EDTA chelated coppers are then sequentially added, is stirred and evenly mixed, is sought
Support agent；
（23）By step（21）Gained expanded vermiculite puts into step（22）Soaked 24 hours in gained nutritional agents, obtain expanded vermiculite
Suspension.
Step（22）In, sodium humate, poly aluminium iron silicate, the mass volume ratio of EDTA chelated coppers and water are 10g： 3g：
0.6g：100mL.
Step（3）Using sprinkling injection during middle injection clean water, it is 6 hours to control injection length.
The injected volume of complex microorganism and composite reactive enzyme is respectively 2% and 4% of water weight in pond.
The preparation method of complex microorganism is as follows：After photosynthetic bacteria, colloid bacillus cereus, trichoderma harzianum individually fermentation
The zymotic fluid of each strain is respectively obtained, then mixed fermentation is produced.Photosynthetic bacteria, colloid bacillus cereus, Kazakhstan in complex microorganism
The strain content of thatch Trichoderma is followed successively by 5,000,000,000/g, 3,000,000,000/g, 2,000,000,000/g.The culture medium used when independent fermentation or mixed fermentation
For potato culture.Fermentation temperature is 48 DEG C, and fermentation time is 24 hours.
The preparation method of composite reactive enzyme is as follows：By neutral proteinase, amylase, lipase and cellulase according to quality
Than 1：1：1：1 is well mixed, and the mass ratio 1 of 4 times of gross weights is then admixed in the lump：1 montmorillonite mixed is mixed with concavo-convex rod soil
In thing.Neutral proteinase, amylase, the enzyme activity of lipase and cellulase be followed successively by 120,000 U/g, 5000U/g, 10,000 U/g and
120000 U/g.
Embodiment 3：
It is a kind of to improve the method for culture environment of aquatic products, comprise the following steps：
（1）Water in emptying pond, clears up pond bottom, introduces clean water to depth of water 6cm, electrochemical water of splashing is carried out at sterilization
Manage, again the water in emptying pond；
（2）One layer of bottom of pond material is uniformly laid in pond bottom, one layer of expanded vermiculite suspension is then uniformly sprayed；
（3）Clean water is slowly injected into more than depth of water 1.5m, complex microorganism and composite reactive enzyme are then delivered into pond, i.e.,
Can.
Wherein, step（1）Electrochemical water be using electro-chemical water processing equipment mass concentration is not less than 2 ‰ sodium chloride
Solution is prepared, and indices are as follows：Effective chlorine mass concentration is 85mg/L, and ozone 4mg/L, pH are 7, oxidation-reduction potential
For 1000mV, hydrogen peroxide 18mg/L.
Cleaning pond bottom includes：The mud and debris of pond bottom are removed, and is washed away 4 times with clean water.
The amount of splashing of electrochemical water is 3% of water weight in pond.Electrochemical treatment time of water is 3 hours.
Step（2）The laying depth of middle bottom of pond material is 10cm.
Bottom of pond material is mixed by the component of following parts by weight：100 parts of river sand, 40 parts of flyash, fertile soil 15
Part, 15 parts of bentonite.
The fountain height of expanded vermiculite suspension is 3L/m2.Expanded vermiculite suspension, which is prepared by the following method, to be obtained：
（21）Crude vermiculite is taken to be heated 220 minutes in 450 DEG C, natural cooling obtains expanded vermiculite；
（22）Sodium humate is soluble in water, poly aluminium iron silicate and EDTA chelated coppers are then sequentially added, is stirred and evenly mixed, is sought
Support agent；
（23）By step（21）Gained expanded vermiculite puts into step（22）Soaked 20 hours in gained nutritional agents, obtain expanded vermiculite
Suspension.
Step（22）In, sodium humate, poly aluminium iron silicate, the mass volume ratio of EDTA chelated coppers and water are 9g：2：0.6g：
100mL。
Step（3）Using sprinkling injection during middle injection clean water, it is 5 hours to control injection length.
The injected volume of complex microorganism and composite reactive enzyme is respectively 1.5% and 3.5% of water weight in pond.
The preparation method of complex microorganism is as follows：After photosynthetic bacteria, colloid bacillus cereus, trichoderma harzianum individually fermentation
The zymotic fluid of each strain is respectively obtained, then mixed fermentation is produced.Photosynthetic bacteria, colloid bacillus cereus, Kazakhstan in complex microorganism
The strain content of thatch Trichoderma is followed successively by 4,500,000,000/g, 2,500,000,000/g, 1,800,000,000/g.The culture medium used when independent fermentation or mixed fermentation
For LB culture mediums.Fermentation temperature is 47 DEG C, and fermentation time is 22 hours.
The preparation method of composite reactive enzyme is as follows：By neutral proteinase, amylase, lipase and cellulase according to quality
Than 1：1：1：1 is well mixed, and the mass ratio 1 of 3 times of gross weights is then admixed in the lump：1 montmorillonite mixed is mixed with concavo-convex rod soil
In thing.Neutral proteinase, amylase, the enzyme activity of lipase and cellulase be followed successively by 120,000 U/g, 5000U/g, 10,000 U/g and
120000 U/g.
Comparative example 1
It is a kind of to improve the method for culture environment of aquatic products, comprise the following steps：
（1）Water in emptying pond, clears up pond bottom；
（2）One layer of bottom of pond material is uniformly laid in pond bottom, one layer of expanded vermiculite suspension is then uniformly sprayed；
（3）Clean water is slowly injected into more than depth of water 1.5m, complex microorganism and composite reactive enzyme are then delivered into pond, i.e.,
Can.
Wherein, step（2）The laying depth of middle bottom of pond material is 10cm.
Bottom of pond material is mixed by the component of following parts by weight：100 parts of river sand, 40 parts of flyash, fertile soil 15
Part, 15 parts of bentonite.
The fountain height of expanded vermiculite suspension is 3L/m2.Expanded vermiculite suspension, which is prepared by the following method, to be obtained：
（21）Crude vermiculite is taken to be heated 220 minutes in 450 DEG C, natural cooling obtains expanded vermiculite；
（22）Sodium humate is soluble in water, poly aluminium iron silicate and EDTA chelated coppers are then sequentially added, is stirred and evenly mixed, is sought
Support agent；
（23）By step（21）Gained expanded vermiculite puts into step（22）Soaked 20 hours in gained nutritional agents, obtain expanded vermiculite
Suspension.
Step（22）In, sodium humate, poly aluminium iron silicate, the mass volume ratio of EDTA chelated coppers and water are 9g：2：0.6g：
100mL。
Step（3）Using sprinkling injection during middle injection clean water, it is 5 hours to control injection length.
The injected volume of complex microorganism and composite reactive enzyme is respectively 1.5% and 3.5% of water weight in pond.
The preparation method of complex microorganism is as follows：After photosynthetic bacteria, colloid bacillus cereus, trichoderma harzianum individually fermentation
The zymotic fluid of each strain is respectively obtained, then mixed fermentation is produced.Photosynthetic bacteria, colloid bacillus cereus, Kazakhstan in complex microorganism
The strain content of thatch Trichoderma is followed successively by 4,500,000,000/g, 2,500,000,000/g, 1,800,000,000/g.The culture medium used when independent fermentation or mixed fermentation
For LB culture mediums.Fermentation temperature is 47 DEG C, and fermentation time is 22 hours.
The preparation method of composite reactive enzyme is as follows：By neutral proteinase, amylase, lipase and cellulase according to quality
Than 1：1：1：1 is well mixed, and the mass ratio 1 of 3 times of gross weights is then admixed in the lump：1 montmorillonite mixed is mixed with concavo-convex rod soil
In thing.Neutral proteinase, amylase, the enzyme activity of lipase and cellulase be followed successively by 120,000 U/g, 5000U/g, 10,000 U/g and
120000 U/g.
Comparative example 2
It is a kind of to improve the method for culture environment of aquatic products, comprise the following steps：
（1）Water in emptying pond, clears up pond bottom, introduces clean water to depth of water 6cm, electrochemical water of splashing is carried out at sterilization
Manage, again the water in emptying pond；
（2）One layer of bottom of pond material is uniformly laid in pond bottom；
（3）Clean water is slowly injected into more than depth of water 1.5m, complex microorganism and composite reactive enzyme are then delivered into pond, i.e.,
Can.
Wherein, step（1）Electrochemical water be using electro-chemical water processing equipment mass concentration is not less than 2 ‰ sodium chloride
Solution is prepared, and indices are as follows：Effective chlorine mass concentration is 85mg/L, and ozone 4mg/L, pH are 7, oxidation-reduction potential
For 1000mV, hydrogen peroxide 18mg/L.
Cleaning pond bottom includes：The mud and debris of pond bottom are removed, and is washed away 4 times with clean water.
The amount of splashing of electrochemical water is 3% of water weight in pond.Electrochemical treatment time of water is 3 hours.
Step（2）The laying depth of middle bottom of pond material is 10cm.
Bottom of pond material is mixed by the component of following parts by weight：100 parts of river sand, 40 parts of flyash, fertile soil 15
Part, 15 parts of bentonite.
Step（3）Using sprinkling injection during middle injection clean water, it is 5 hours to control injection length.
The injected volume of complex microorganism and composite reactive enzyme is respectively 1.5% and 3.5% of water weight in pond.
The preparation method of complex microorganism is as follows：After photosynthetic bacteria, colloid bacillus cereus, trichoderma harzianum individually fermentation
The zymotic fluid of each strain is respectively obtained, then mixed fermentation is produced.Photosynthetic bacteria, colloid bacillus cereus, Kazakhstan in complex microorganism
The strain content of thatch Trichoderma is followed successively by 4,500,000,000/g, 2,500,000,000/g, 1,800,000,000/g.The culture medium used when independent fermentation or mixed fermentation
For LB culture mediums.Fermentation temperature is 47 DEG C, and fermentation time is 22 hours.
The preparation method of composite reactive enzyme is as follows：By neutral proteinase, amylase, lipase and cellulase according to quality
Than 1：1：1：1 is well mixed, and the mass ratio 1 of 3 times of gross weights is then admixed in the lump：1 montmorillonite mixed is mixed with concavo-convex rod soil
In thing.Neutral proteinase, amylase, the enzyme activity of lipase and cellulase be followed successively by 120,000 U/g, 5000U/g, 10,000 U/g and
120000 U/g.
Comparative example 3
It is a kind of to improve the method for culture environment of aquatic products, comprise the following steps：
（1）Water in emptying pond, clears up pond bottom, introduces clean water to depth of water 6cm, electrochemical water of splashing is carried out at sterilization
Manage, again the water in emptying pond；
（2）One layer of bottom of pond material is uniformly laid in pond bottom, one layer of expanded vermiculite suspension is then uniformly sprayed；
（3）Clean water is slowly injected into more than depth of water 1.5m, complex microorganism and composite reactive enzyme are then delivered into pond, i.e.,
Can.
Wherein, step（1）Electrochemical water be using electro-chemical water processing equipment mass concentration is not less than 2 ‰ sodium chloride
Solution is prepared, and indices are as follows：Effective chlorine mass concentration is 85mg/L, and ozone 4mg/L, pH are 7, oxidation-reduction potential
For 1000mV, hydrogen peroxide 18mg/L.
Cleaning pond bottom includes：The mud and debris of pond bottom are removed, and is washed away 4 times with clean water.
The amount of splashing of electrochemical water is 3% of water weight in pond.Electrochemical treatment time of water is 3 hours.
Step（2）The laying depth of middle bottom of pond material is 10cm.
Bottom of pond material is mixed by the component of following parts by weight：100 parts of river sand, 15 parts of fertile soil, bentonite 15
Part.
The fountain height of expanded vermiculite suspension is 3L/m2.Expanded vermiculite suspension, which is prepared by the following method, to be obtained：
（21）Crude vermiculite is taken to be heated 220 minutes in 450 DEG C, natural cooling obtains expanded vermiculite；
（22）Sodium humate is soluble in water, poly aluminium iron silicate and EDTA chelated coppers are then sequentially added, is stirred and evenly mixed, is sought
Support agent；
（23）By step（21）Gained expanded vermiculite puts into step（22）Soaked 20 hours in gained nutritional agents, obtain expanded vermiculite
Suspension.
Step（22）In, sodium humate, poly aluminium iron silicate, the mass volume ratio of EDTA chelated coppers and water are 9g：2：0.6g：
100mL。
Step（3）Using sprinkling injection during middle injection clean water, it is 5 hours to control injection length.
The injected volume of complex microorganism and composite reactive enzyme is respectively 1.5% and 3.5% of water weight in pond.
The preparation method of complex microorganism is as follows：After photosynthetic bacteria, colloid bacillus cereus, trichoderma harzianum individually fermentation
The zymotic fluid of each strain is respectively obtained, then mixed fermentation is produced.Photosynthetic bacteria, colloid bacillus cereus, Kazakhstan in complex microorganism
The strain content of thatch Trichoderma is followed successively by 4,500,000,000/g, 2,500,000,000/g, 1,800,000,000/g.The culture medium used when independent fermentation or mixed fermentation
For LB culture mediums.Fermentation temperature is 47 DEG C, and fermentation time is 22 hours.
The preparation method of composite reactive enzyme is as follows：By neutral proteinase, amylase, lipase and cellulase according to quality
Than 1：1：1：1 is well mixed, and the mass ratio 1 of 3 times of gross weights is then admixed in the lump：1 montmorillonite mixed is mixed with concavo-convex rod soil
In thing.Neutral proteinase, amylase, the enzyme activity of lipase and cellulase be followed successively by 120,000 U/g, 5000U/g, 10,000 U/g and
120000 U/g.
Test example
Selection has the leg Shrimp Litopenaeus vannamei Culture Raceway-type Ponds pool of 3 years cultivation history, and bottom material of pond for breeding is sandy soil, is isolated into 7 of identical size
Little pond, wherein an optional little pond is without any processing as a control group, remaining little pond at random using embodiment 1~
3 or the method for comparative example 1~3 handled, processing is thrown seedling for 3 days after terminating and raised, and the culture-cycle is 60 days, and the culture-cycle terminates
Afterwards, the water quality situation of each little pond and the cultivation situation of Penaeus Vannmei are investigated, 1~table 2 is the results are shown in Table.
Wherein, photobacteria detection method of toxicity is as follows：UV254 is determined：With 0.4 μm of micro porous filtration, ultraviolet spectrometry is utilized
Photometer direct reading under 254nm wavelength.Using the photobacterium phosphoreum of SDI companies of the U.S.
（PhotobacteriumphoremT3）Freeze-dried powder, determines different water sample luminous intensities, and light inhibiting rate（%）=（In 1- water samples
Luminous intensity in luminous intensity/blank control）×100%.
The water quality situation of each little pond of table 1.
| Ammonia-nitrogen content（mg/L） | Hydrogen sulfide content（mg/L） | COD concentration（mg/L） | Unit area pasture and water increment（g/m2） | UV254 | Luminous inhibiting rate（%） | |
| Embodiment 1 | 0.15 | 0.08 | 0.12 | 1089 | 1.5 | 10.3 |
| Embodiment 2 | 0.15 | 0.07 | 0.13 | 1088 | 1.4 | 10.3 |
| Embodiment 3 | 0.12 | 0.06 | 0.1 | 1106 | 1.2 | 10 |
| Comparative example 1 | 2.6 | 2.1 | 15.3 | 456 | 8.7 | 25.6 |
| Comparative example 2 | 2.4 | 2.1 | 12.9 | 478 | 8.7 | 24.8 |
| Comparative example 3 | 0.89 | 0.97 | 1.89 | 788 | 2.6 | 13.4 |
| Control group | 3.5 | 2.8 | 18.9 | 213 | 12.3 | 35.3 |
As shown in Table 1, the treated pond of embodiment 1~3, it is after the cultivation of a culture-cycle, ammonia-nitrogen content, vulcanization
Hydrogen content and COD concentration are low, and unit area pasture and water increment is high, with lower UV254 and luminous inhibiting rate, illustrates its water quality
More preferably, far superior to control group and comparative example 1~3.
The cultivation situation of the Penaeus Vannmei of table 2.
| Per mu yield（Kg/ mus） | Average single weight（g） | |
| Embodiment 1 | 1003 | 32 |
| Embodiment 2 | 1002 | 32 |
| Embodiment 3 | 1012 | 35 |
| Comparative example 1 | 228 | 20 |
| Comparative example 2 | 413 | 21 |
| Comparative example 3 | 876 | 24 |
| Control group | 132 | 17 |
As shown in Table 2, the little pond after embodiment 1~3 is handled has higher Penaeus Vannmei per mu yield, and average single
Weight is also larger, and far superior to control group and comparative example 1~3, particularly comparative example 1 and control group occur in that a large amount of shrimps death
Situation.
Although the above-mentioned embodiment to the present invention is described, not to the limit of the scope of the present invention
System, on the basis of technical scheme, it is each that those skilled in the art need not pay that creative work can make
Plant modification or deform still within protection scope of the present invention.
Claims (10)
1. a kind of improve the method for culture environment of aquatic products, it is characterised in that comprises the following steps：
（1）Water in emptying pond, clears up pond bottom, introduces clean water to 5~8cm of the depth of water, electrochemical water of splashing is sterilized
Handle, again the water in emptying pond；
（2）One layer of bottom of pond material is uniformly laid in pond bottom, one layer of expanded vermiculite suspension is then uniformly sprayed；
（3）Clean water is slowly injected into more than depth of water 1.5m, complex microorganism and composite reactive enzyme are then delivered into pond, i.e.,
Can.
2. a kind of method of improvement culture environment of aquatic products according to claim 1, it is characterised in that step（1）Electrification
Water is that the sodium chloride solution for being not less than 2 ‰ to mass concentration using electro-chemical water processing equipment is prepared, and indices are as follows：
Effective chlorine mass concentration is 70~100mg/L, and ozone 3~4mg/L, pH is 6.5~7.5, oxidation-reduction potential is 900~
1100mV, 15~20mg/L of hydrogen peroxide.
3. a kind of method of improvement culture environment of aquatic products according to claim 1, it is characterised in that step（1）In, cleaning
Pond bottom includes：The mud and debris of pond bottom are removed, and is washed away 3~4 times with clean water.
4. a kind of method of improvement culture environment of aquatic products according to claim 1, it is characterised in that step（1）Middle electrification
The amount of splashing of water is 2~5% of water weight in pond.
5. a kind of method of improvement culture environment of aquatic products according to claim 1, it is characterised in that step（2）In, it is described
Bottom of pond material is mixed by the component of following parts by weight：100 parts of river sand, 30~50 parts of flyash, fertile soil 10~20
Part, 10~20 parts of bentonite.
6. a kind of method of improvement culture environment of aquatic products according to claim 1, it is characterised in that step（2）In, expansion
The fountain height of vermiculite suspension is 2~3L/m2。
7. a kind of method of improvement culture environment of aquatic products according to claim 1, it is characterised in that step（2）Described in
Expanded vermiculite suspension, which is prepared by the following method, to be obtained：
（21）Crude vermiculite is taken to be heated 200~250 minutes in 400~500 DEG C, natural cooling obtains expanded vermiculite；
（22）Sodium humate is soluble in water, poly aluminium iron silicate and EDTA chelated coppers are then sequentially added, is stirred and evenly mixed, is sought
Support agent；
（23）By step（21）Gained expanded vermiculite puts into step（22）Soak 18~24 hours, expanded in gained nutritional agents
Vermiculite suspension.
8. a kind of method of improvement culture environment of aquatic products according to claim 1, it is characterised in that step（3）In, it is combined
The injected volume of microorganism and composite reactive enzyme is respectively 1~2% and 3~4% of water weight in pond.
9. a kind of method of improvement culture environment of aquatic products according to claim 1, it is characterised in that the system of complex microorganism
Preparation Method is as follows：The fermentation of each strain will be respectively obtained after photosynthetic bacteria, colloid bacillus cereus, trichoderma harzianum individually fermentation
Liquid, then mixed fermentation is produced.
10. the method for a kind of improvement culture environment of aquatic products according to claim 1, it is characterised in that composite reactive enzyme
Preparation method is as follows：By neutral proteinase, amylase, lipase and cellulase according to mass ratio 1：1：1：1 is well mixed, so
Admix the mass ratio 1 of 3~4 times of gross weights in the lump afterwards：In 1 montmorillonite mixed and concavo-convex rod soil mixture.
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