CN110547237B - Method for preventing and controlling blue algae bloom in shrimp pond by using oocyst algae - Google Patents
Method for preventing and controlling blue algae bloom in shrimp pond by using oocyst algae Download PDFInfo
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- CN110547237B CN110547237B CN201910949805.3A CN201910949805A CN110547237B CN 110547237 B CN110547237 B CN 110547237B CN 201910949805 A CN201910949805 A CN 201910949805A CN 110547237 B CN110547237 B CN 110547237B
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/20—Prevention of biofouling
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention providesA method for preventing and controlling blue algae bloom in a shrimp pond by using oocyst algae belongs to the technical field of aquaculture, and comprises the following steps: when no blue algae exists in the water body, the oocyst algae is inoculated into the water body for culture until the concentration of the oocyst algae in the water body is more than 1.5 multiplied by 105cell/mL; putting prawn fries for culturing; when 1X 104cell/L<The concentration of the blue algae in the water body is less than 2 multiplied by 107When cell/L, inoculating the concentrated oocyst algae into water body for culture until the concentration of blue algae in the water body is less than 1 × 105And (5) putting the young prawns in the cell/L. The method has obvious prevention and control effects on the harmful cyanobacterial bloom on the basis of stabilizing the water quality, is easy to master, and is simple and convenient to operate; the biological prevention and control means is used, so that the method is mild and effective, the economic cost is low, and the environmental pollution is small.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a method for preventing and controlling cyanobacterial bloom in a shrimp pond by using oocyst algae.
Background
Pond culture of prawns is a traditional culture method in China and is widely used. In the middle and later period of cultivation, water eutrophication is caused by artificial fertilization, excessive feeding and the accumulation of excrement of the cultivated prawns. The blue algae is the dominant microalgae population in the water body, and can grow explosively to form water bloom under the eutrophication condition. After the water bloom is formed, the blue algae can prevent oxygen in the air from dissolving into water, and the dissolved oxygen in the water is greatly consumed under the breathing action of night, so that the dissolved oxygen in the pond is reduced, and the cultured shrimps die due to stress and oxygen deficiency. After the blue algae is propagated in a large amount, the algae die in a large amount, and toxic substances such as hydrogen sulfide and the like generated by decomposition of corpses threaten the health of the prawns. Relevant experiments prove that the microcystin contained in the blue algae can cause the immune system of the prawns to be reduced. Cyanobacteria often produce multiple toxins, which may act synergistically. The prawn eats toxic blue algae by mistake, or can accumulate trace amount of toxin in the environment in vivo by respiration and other modes to cause chronic poisoning. Cyanobacterial bloom is also one of the causes of prawn early death syndrome (EMS).
From the discovery of the cyanobacterial bloom to the complete outbreak, no effective method can treat the cyanobacterial bloom at present. It is feasible to prevent and control the cyanobacterial bloom in advance. The method for preventing and controlling the cyanobacterial bloom by directionally cultivating the microalgae is a safe, economic and effective method. The ecological niche overlapping exists between a plurality of microalgae and the blue algae, and the competition relationship exists between the microalgae and the blue algae. Microalgae can gain a competitive advantage by inhibiting the growth of other phytoplankton through allelochemicals secreted by themselves, and this biochemical reaction is called allelochemicals. Allelochemicals such as phenolic acids, fatty acids, tannins, lactones, terpenes, alkaloids, and flavonoid sulfides have been isolated and proven effective. Allelopathy mainly inhibits the growth of other microalgae through the following aspects: 1. photosynthesis is one of the most important physiological processes in the growth process of algae. Under the action of allelochemicals, algal photosynthetic pigments and certain protein complexes are affected. For example, chlorophyll degradation is accelerated, phycobilirubins are destroyed, and chlorophyll content is decreased because chlorophyll synthesis is inhibited. Meanwhile, allelochemicals can block the electron transfer in the algae photosynthetic system, thereby influencing the synthesis of Adenosine Triphosphate (ATP). 2. Cell membranes are important barriers for the exchange of substances and energy between cells and the external environment. Research shows that main fatty acid in some algae cell membranes is oxidized under the action of allelochemicals, and the unsaturation degree is increased, so that the fluidity of the cell membranes is improved, and the selective permeability of the cell membranes is reduced. In addition, when exposed to a certain concentration of allelochemicals, the shape of the cells changes, i.e., protoplasts shrink and separate from the cell wall, the nucleus shrinks, the structure of the chloroplast is destroyed, and the algae cannot photosynthesize and finally die. 3. Changes in the function of enzymes, which directly or indirectly affect the growth and physiological properties of the body, play a crucial role in the body. Allelochemicals can affect the activities of superoxide dismutase (SOD), Catalase (CAT), reduced Glutathione (GSH) and ascorbic acid (AsA) to various degrees, and inhibit algae. Related studies have shown that substances, such as aloe vera extract, can even inhibit the production of cyanobacterial toxins. 4. Allelochemicals may also inhibit the growth of algae by inhibiting their respiration, protein synthesis and gene expression. Most alkaloids have allelopathic effects and can tightly bind with target DNA, resulting in increased DNA 5c cleavage temperature, thereby inhibiting translation and transcription of DNA, and affecting final synthesis of protein. In addition, the site of action and the strength vary with allelochemicals, and multiple targets can be selected to affect cell mitosis. 5. Allelochemicals can inhibit algae growth by promoting the synthesis of free radicals and Reactive Oxygen Species (ROS). Free radicals cause damage by destroying nucleic acids, lipids, and proteins.
The beneficial microalgae is directionally selected, so that the cyanobacterial bloom can be prevented, and the pond water quality can be effectively regulated and controlled. The growth of microalgae needs to absorb and utilize nutrient substances such as nitrogen, phosphorus and the like in the water environment, and meanwhile, the photosynthesis of microalgae cells can absorb and remove toxic pollutants in the water body, so that the microalgae has the functions of improving dissolved oxygen in the water body and maintaining stable pH. However, the variety of microalgae is various, and at present, there is no definite microalgae capable of effectively preventing and treating the cyanobacterial bloom and a mature method for preventing and treating the cyanobacterial bloom by using the microalgae.
Disclosure of Invention
In view of the above, the present invention aims to provide a method for controlling cyanobacterial bloom in a shrimp pond by using oocyst algae; the method is simple and convenient to operate, can maintain the stability of the ecological system of the culture water body for a long time, inhibits the propagation of harmful blue-green algae, can reduce diseases of culture objects, improves the yield of prawns, reduces the culture cost, and has higher economic benefit.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a method for preventing and controlling cyanobacterial bloom in a shrimp pond by using oocyst algae, which comprises the following steps:
when no blue-green algae exists in the water body,
inoculating the strain of oocyst algae in water to culture until the concentration of the oocyst algae in the water is more than 1.5 × 105cell/mL; putting prawn fries for culturing; the concentration of the oocyst algae is 1-1.5 multiplied by 105cell/mL, the inoculation amount is 10m3/1000m3；
1×104cell/L<The concentration of the blue algae in the water body is less than 2 multiplied by 107When the cell is in a cell/L state,
inoculating the concentrated oocyst algae into water body for culture until the concentration of blue algae in the water body is less than 1 × 105When cell/L is needed, putting prawn fries;
the concentration of the concentrated oocystis is more than 1 × 107cell/mL, the inoculation amount is more than 0.06m3/1000m3。
Preferably, aeration and oxygenation are carried out in the oocyst algae culture process; the aeration and oxygenation are carried out by adopting an aerator; the rated voltage of the aerator is 220V/380V; the power of the aerator is 1400-1600W.
Preferably, the time of aeration and oxygenation is 1.5-2.5 h/time and 1-3 times per day.
Preferably, putting prawn seedlings, and adding inorganic fertilizer into the water body every 14-16 days; the inorganic fertilizer is a compound fertilizer.
Preferably, the addition amount of the inorganic fertilizer is 2.5-3 kg/1000m3。
Preferably, the oocyst algae species are oocyst algae after intermediate expansion culture in a culture tank; adding nutrient substances into the water body in the intermediate expanded culture process; the nutrient comprises the following components:
30-35 g/m potassium nitrate340-45 g/m sodium dihydrogen phosphate30.2-0.5 g/m ferric citrate3Vitamin B120.5-1 g/m30.1-0.2 g/m magnesium sulfate heptahydrate30.05-0.1 g/m calcium chloride3Vitamin B1 0.5～1g/m3Arginine 0.01-0.05 g/m30.01-0.02 g/m alanine35-10 g/m of nicotinamide3Inositol 80-100 g/m3Biotin 2-5 g/m31-3 g/m of thoraco-m-azobenzene3。
Preferably, aeration is carried out in the intermediate expansion culture process; the aeration rate of the aeration is 0.6-0.8 vvm; the aeration is uninterrupted aeration.
Preferably, the absence of blue algae in the water body is realized by thoroughly disinfecting a pond and precipitating and filtering culture water; thorough disinfection includes in proper order that the pond insolate, the desilting spills the lime, and water injection bubble pond washs, bleaching powder disinfection and drainage.
Preferably, the usage amount of the quicklime is 50-70 kg/mu.
Preferably, the usage amount of the bleaching powder is 45-55 kg/1000m3。
The invention has the beneficial effects that: the method for preventing and controlling the blue algae bloom in the shrimp pond by using the oocyst algae has the advantages that the blue algae is obviously inhibited by the oocyst algae, the ecological environment is friendly, the growth stability can be kept for a long time in the pond culture environment of the prawns, the oriented culture can be carried out, the oocyst algae with higher purity is cultured in the indoor culture, and then the oocyst algae are transferred to the culture water body for the oriented culture. When the pond has blue algae, but the scale of the blue algae is not large, the microalgae is used for preventing and controlling outbreak; compared with the traditional method for preventing the cyanobacteria bloom in the middle and later stages of cultivation due to the fact that the dominant algae species in the water body are not noticed during water fertilizing, the method disclosed by the invention has the advantages that the directional selection of the cultivated algae species is noticed, the remarkable prevention and control effects on the harmful cyanobacteria bloom are realized on the basis of stabilizing the water quality, the grasping is easy, and the operation is simple and convenient. Compared with the powerful algicide, the method uses a biological prevention and control means, is mild and effective, has low economic cost and causes little pollution to the environment.
By using the method, the water in the culture pond is dark green, the transparency is moderate, the ecological system can keep balance for a long time, the pH value is stable at 7.5-8.5, and the Chemical Oxygen Demand (COD)>5, ammonia nitrogen is less than or equal to 1, total phosphorus is less than or equal to 0.2, fluoride is less than or equal to 1, and total coliform bacteria per bacterium per L-1Not more than 5000 per microcystis aeruginosa/per mL-1< 1000; the method can effectively inhibit the outbreak growth of harmful algae species in the whole culture process.
Detailed Description
The invention provides a method for preventing and controlling cyanobacterial bloom in a shrimp pond by using oocyst algae, which comprises the following steps: when no blue algae exists in the water body, the oocyst algae is inoculated into the water body for culture until the concentration of the oocyst algae in the water body is more than 1.5 multiplied by 105cell/mL; putting prawn fries for culturing; the concentration of the oocyst algae is 1-1.5 multiplied by 105cell/mL, the inoculation amount is 10m3/1000m3(ii) a When 1X 104cell/L<The concentration of the blue algae in the water body is less than 2 multiplied by 107When cell/L, inoculating the concentrated oocyst algae into water body for culture until the concentration of blue algae in the water body is less than 1 × 105When cell/L is needed, putting prawn fries; the concentration of the concentrated oocystis is more than 1 × 107cell/mLThe inoculation amount is more than 0.06m3/1000m3。
In the invention, when no blue algae exists in the water body, the oocyst algae is inoculated in the water body for culture until the concentration of the oocyst algae in the water body is more than 1.5 multiplied by 105cell/mL; and putting the young prawns into the culture tank for culture. In the invention, the water body is preferably pond water body, and the absence of blue algae in the water body is realized by thoroughly sterilizing the pond and precipitating and filtering culture water. In the invention, the thorough disinfection sequentially comprises the steps of insolation, desilting, quicklime splashing, water injection and soaking pond cleaning, bleaching powder disinfection and drainage. In the invention, the exposure time is preferably 7-10 d, and the dredging can be carried out by adopting conventional operation in the field. In the invention, the usage amount of the quicklime is preferably 50-70 kg/mu, and more preferably 55-65 kg/mu; the preferable usage amount of the bleaching powder is 45-55 kg/1000m3More preferably 50kg/1000m3(ii) a The invention preferably drains water after 3d disinfection of the bleaching powder. In the invention, the water for cultivation is precipitated and filtered as follows: firstly, the culture water is injected into a sand filter and then is filtered and collected (large particles are removed), the collected water is injected into a sedimentation tank, a baffle of the sedimentation tank is covered to shade after the water is injected, dark sedimentation is carried out (floating algae can freely precipitate in dark environment), and the water body is clarified after the dark sedimentation is carried out for 2d and can be used.
In the invention, the oocyst algae species are oocyst algae subjected to intermediate expansion culture in a culture tank. Before the intermediate expanded culture, the culture barrel is preferably sterilized, and in the invention, strong chlorine is preferably used for the sterilization; the preferred usage amount of the strong chlorine essence is 8-12 g/m3More preferably 10g/m3(ii) a After the strong chlorine is used for disinfection for 1d, sodium thiosulfate is preferably adopted to neutralize residual chlorine in water, and aeration is carried out for 6-8 h. In the present invention, the amount of sodium thiosulfate used is determined according to the amount of residual chlorine in water, for example, when the concentration of residual chlorine in water is 1ppm, the corresponding amount of sodium thiosulfate used is 1.6g/m3(ii) a The sodium thiosulfate is preferably used in the form of a sodium thiosulfate solution, the concentration of which is preferably such that the sodium thiosulfate solution is dissolved in water90-110 g/L, more preferably 100 g/L. According to the invention, after the culture barrel is disinfected, culture water is added into the culture barrel, and the culture water is filtered by a 60-mesh bolting silk screen after being filtered by a sand filter.
In the present invention, the concentration of the inoculated algal species in the intermediate expansion culture is preferably more than 1X 107cell/mL; the volume of the inoculated algae seeds is preferably 0.7-0.9L/m3. In the intermediate expanded culture process, nutrient substances are added into a water body; the nutrient comprises the following components: potassium nitrate 35g/m345g/m sodium dihydrogen phosphate30.5g/m ferric citrate3Vitamin B120.5g/m3Magnesium sulfate heptahydrate 0.15g/m3Calcium chloride 0.05g/m3Vitamin B11g/m3Arginine 0.01g/m3Alanine 0.02g/m3Nicotinamide 10g/m3Inositol 100g/m3Biotin 5g/m33g/m of transthoracic nitrobenzene3. In the present invention, aeration is preferably performed during the intermediate amplification culture; the aeration rate of aeration is preferably 0.6-0.8 vvm, and more preferably 0.7 vvm; the aeration is continuous and uninterrupted aeration; the aeration has the function of preventing the algae from sinking and enabling the algae to receive light uniformly.
After the intermediate culture is finished, the algae seeds obtained by the intermediate culture are inoculated in a water body for culture until the concentration of the oocyst algae in the water body is more than 1.5 multiplied by 105cell/mL; and putting the young prawns into the culture tank for culture. In the invention, the time for culturing the oocyst algae is preferably 7-8 d; the invention carries out aeration and oxygenation in the process of culturing the oocyst algae; the aeration and oxygenation are carried out by adopting an aerator; the rated voltage of the aerator is 220V/380V; the power of the aerator is preferably 1400-1600W, and more preferably 1500W. In the invention, the time of aeration and oxygenation is preferably 1.5-2.5 h/time, 1-3 times per day, more preferably 2 h/time, and 2 times per day; the specific time of aeration and oxygenation is preferably 8-10 hours in the morning and 16-18 hours in the afternoon every day. The invention has no special limit on the amount of the shrimp larvae put in and the operation steps, and adopts the conventional operation in the field, namelyCan be prepared. After prawn seedlings are thrown in the water, inorganic fertilizer is added into the water every 14-16 days; the inorganic fertilizer is preferably a compound fertilizer; the addition amount of the inorganic fertilizer is preferably 2.5-3 kg/1000m3。
In the present invention, when 1X 104cell/L<The concentration of the blue algae in the water body is less than 2 multiplied by 107When cell/L, inoculating the concentrated oocyst algae into water body for culture until the concentration of blue algae in the water body is less than 1 × 105When cell/L is needed, putting prawn fries; the concentration of the concentrated oocystis is more than 1 × 107cell/mL, the inoculation amount is more than 0.06m3/1000m3。
In the invention, the steps aim at that the blue algae in the water body has developed to a certain scale but does not reach the dangerous value causing rapid water bloom in the water fertilizing process (<2×107cell/L); when the concentration of the blue algae in the water body is less than 1 multiplied by 107cell/L, preferably performing intermediate amplification culture on the concentrated oocyst for 1-2 d, and then inoculating; the steps and parameters of the intermediate expanded culture are consistent with those of the culture barrel culture, and are not described in detail herein. When in the water body is 1 x 107cell/L<The concentration of the blue algae in the water body is less than 2 multiplied by 107When cell/L, directly inoculating concentrated oocyst algae strains into the water body for culture until the concentration of blue algae in the water body is less than 1 × 105And (5) putting the young prawns in the cell/L.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
(1) Middle culture of oocyst algae in a vat: preparing four large breeding barrels with the diameter of 3m, wherein the height of each barrel is 1.2m, after the large barrels are cleaned, filtering the breeding water by using a sand filter, filtering the breeding water by using a 60-mesh bolting silk screen, and injecting the water into the barrels, wherein the depth of the pond water is 0.8-1 m. And (3) disinfecting the water body by using strong chlorine, neutralizing the surplus chlorine by using sodium thiosulfate after one day, and aerating for 6-8 hours to remove the surplus chlorine. Adding potassium nitrate (KNO) required for growth of oocyst algae after residual chlorine completely disappears3)35g/m3Sodium dihydrogen phosphate (NaH)2PO4)45g/m3Ferric citrate 0.5g/m3Vitamin B120.5g/m3Magnesium sulfate heptahydrate (MgSO)4·7H2O)0.15g/m3Calcium chloride (CaCl)2)0.05g/m3Vitamin B11 g/m3Arginine 0.01g/m3Alanine 0.02g/m3Nicotinamide 10g/m3Inositol 100g/m3Biotin 5g/m33g/m of thoracenaphthene3. Each vat is connected into 5L 1X 107cell/mL or more of the concentrated algae solution.
(2) Culturing oocyst algae in a shrimp pond: pool area 2000m2Placing two waterwheel type oxygen increasing machines (rated voltage 220V/380V; power 1500W) at opposite angles of the shrimp pond when the water depth is 1.5-2.0 m; after the large barrel of oocyst algae grows to a certain density, thoroughly cleaning and sterilizing the pond, adding 1.2m of culture water subjected to precipitation and filtration, and then adding 12g/m of culture water3Applying the composite fertilizer in proportion, starting an aerator to fully mix the water in the pond, finally moving the oocysts cultured in a vat to the pond for culturing for 7-8 days to enable the oocysts to become dominant species in the pond ((>1.5×105cell/ml), the shrimp seeds can be thrown.
The culture process is stable and dark green in water color, the average value of ammonia nitrogen content is 0.075mg/L, the average value of nitrite is 0.015mg/L, the pH average value is 8.7, and the peak value of microcystis is 1 multiplied by 103cell/L, no algal bloom is generated in the whole process.
Example 2
Culturing oocyst algae, blue algae and shrimp pond: when a block is 2000m3Microcystis aeruginosa with concentration of 1 × 10 is observed in the shrimp pool after the water is fertilized7cell/L, discharging 1/3 of pool water. Adding 60L of the mixture with the concentration of 1 multiplied by 107cell/mL of concentrated algal species. Starting the aerator for 2h at 8 am and 16 pm every day until the number of blue algae in water declines to less than 1 × 105And only cell/L, the shrimp larvae can be thrown.
After 13 days of water control, the quantity of the microcystis is stably controlled to gradually decrease and finally is stabilized at 1 × 104cell/L, ammonia nitrogen content average value of 0.081mg/L, nitrite average value of 0.007mg/L, PH average value of 8.3, and no algal bloom in the whole process.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (5)
1. A method for preventing and controlling cyanobacterial bloom in a shrimp pond by using oocyst algae comprises the following steps:
when no blue-green algae exists in the water body,
inoculating the strain of oocyst algae in water to culture until the concentration of the oocyst algae in the water is more than 1.5 × 105cell/mL; putting prawn fries for culturing; the concentration of the oocyst algae is 1-1.5 multiplied by 105cell/mL, wherein the inoculation amount of the oocyst algae is 10m3/1000m3；
When 1X 104cell/L<Concentration of blue algae in water<2×107When the cell is in a cell/L state,
inoculating the concentrated oocyst algae into water body for culture until the concentration of blue algae in the water body<1×105When cell/L is needed, putting prawn fries;
the concentration of the concentrated oocystis is more than 1 × 107cell/mL, the inoculation amount is more than 0.06m3/1000m3；
The oocyst algae seeds and the concentrated oocyst algae seeds are both subjected to intermediate expanded culture in a culture bucket;
aerating and oxygenating the oocyst algae seeds and the concentrated oocyst algae seeds in the water body culture process; the aeration and oxygenation are carried out by adopting an aerator; the rated voltage of the aerator is 220V/380V; the power of the aerator is 1400-1600W;
the time of aeration and oxygenation is 1.5-2.5 h/time, and 1-3 times per day;
after putting the prawn seedlings, adding inorganic fertilizer into the water body every 14-16 days; the inorganic fertilizer is a compound fertilizer, and the addition amount of the inorganic fertilizer is 2.5-3 kg/1000m3；
Adding nutrient substances into the water body in the intermediate expanded culture process; the nutrient comprises the following components:
30-35 wt% of potassium nitrateg/m340-45 g/m sodium dihydrogen phosphate30.2-0.5 g/m ferric citrate3Vitamin B120.5-1 g/m30.1-0.2 g/m magnesium sulfate heptahydrate30.05-0.1 g/m calcium chloride3Vitamin B10.5-1 g/m3Arginine 0.01-0.05 g/m30.01-0.02 g/m alanine35-10 g/m of nicotinamide3Inositol 80-100 g/m3Biotin 2-5 g/m31-3 g/m of thoraco-m-azobenzene3。
2. The method according to claim 1, wherein aeration is performed during the intermediate expanded culture; the aeration rate of the aeration is 0.6-0.8 vvm; the aeration is uninterrupted aeration.
3. The method of claim 1, wherein the absence of cyanobacteria in the body of water is achieved by thorough disinfection of ponds and sedimentation filtration of aquaculture water; thorough disinfection includes in proper order that the pond insolate, the desilting spills the lime, and water injection bubble pond washs, bleaching powder disinfection and drainage.
4. The method as claimed in claim 3, wherein the amount of quicklime used is 50 to 70 kg/acre.
5. The method according to claim 3, wherein the bleaching powder is used in an amount of 45-55 kg/1000m3。
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| CN103931481A (en) * | 2014-05-13 | 2014-07-23 | 广东海洋大学 | Method for cultivating amphiprora alata used for regulating water quality in higher-place pond prawn cultivation |
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