CN105112338A - Method capable of utilizing lake cyanobacterial blooms to culture planktonic algae - Google Patents
Method capable of utilizing lake cyanobacterial blooms to culture planktonic algae Download PDFInfo
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Abstract
The invention relates to a method capable of utilizing lake cyanobacterial blooms to culture planktonic algae, firstly cyanobacterial bloom pulp is obtained from a lake in a season that the cyanobacterial blooms happen in the lake, then cyanobacteria pulp is aerated or stirred to guarantee a local aerobic zone to form in the cyanobacteria pulp, rotten cyanobacteria pulp is transferred into a container with tap water to dilute after the cyanobacteria pulp is treated about 10 days by being ventilated in normal temperature, and the container which is used to store mixed liquor of the tap water and the rotten cyanobacteria pulp is placed in an outdoor opening environment, and is aerated or stirred to keep dissolved oxygen in the mixed liquor above 3mg/L. The container which stores the mixed liquor of the tap water and the rotten cyanobacteria pulp can have the advantage of planktonic algae after the container is aerated or stirred about 10 days. The method capable of utilizing lake cyanobacterial blooms to culture the planktonic algae is simple in culture method, easy to obtain raw materials, convenient to carry out, low in culture cost and wide in appliance range.
Description
Technical field
The present invention relates to the cultural method of plant plankton, be specifically related to a kind of method utilizing lake blue algae wawter bloom to cultivate planktonic green algae.
Background technology
Due to developing rapidly of industrial or agricultural, the raised growth of the raised growth that the multiple water body in lake nitrogen of China, phosphorus concentration have occurred plant plankton while raising especially Cyanophyta plant plankton, and define serious blue-green alga bloom, cause the performance of water body in lake several functions to affect adversely.
Up to the present, blue-green alga bloom is generally taken as objectionable impurities, needs to be removed, and generally removes from lake the mode that blue-green alga bloom takes machinery to salvage.Blue-green alga bloom is salvaged out afterwards through certain process of anhydrating, and then piles up and does not further utilize.Although China can obtain the natural organic fertilizer material of lake blue algae wawter bloom as farm crop in the age that chemical fertilizers production is backward in technique, along with widely using of chemical fertilizer, the lake periphery peasant of breakout of cyanobacteria blooms is had to use chemical fertilizer to substitute natural organic fertilizer material gradually.Therefore, also fewer to the recycling of blue-green alga bloom in China.
Relevant unit of the U.S. buys the concentrated blue-green alga bloom of water ratio about 10% from Wuxi to have news report to say in the recent period, although far away, freight charges are high, and concentrated blue-green alga blooms of these outlets are bought as resource certainly.The wherein concrete technology that utilizes is not reported accordingly.
Blue-green alga bloom was the product of water eutrophication originally, and its absorption have accumulated a large number of nutrients, was a kind of available resource.And green alga is the same with blue-green algae, is all plant plankton, has close ecological niche with blue-green algae, but the utility value had.Green alga can be the food that the zooplankton such as wheel animalcule, cladocera provides high-quality, and green alga and wheel animalcule, cladocera etc. are the open-mouthed baits of fish and shrimp young high-quality, the seed rearing of culture fishery is played an important role, and is the high quality raw material of production of biodiesel compared with multiple types in green alga.
Summary of the invention
The object of the invention is to carry out recycling to lake blue algae wawter bloom, utilize lake blue algae wawter bloom to cultivate planktonic green algae, this cultural method can obtain highdensity Chlorophyta plant plankton, and toxigenic capacity is low, and culturing step simply and easily implement.
In order to realize above technique effect, the present invention realizes as follows:
Utilize lake blue algae wawter bloom to cultivate a method for planktonic green algae, its step comprises:
(1) there is this season of blue-green alga bloom in lake, from lake, obtain blue-green alga bloom, and corresponding filtering is carried out to wherein moisture obtain blue-green alga bloom slurry and blue algae slurry with concentrated blue-green alga bloom;
(2) described blue algae slurry is transferred in container, under being placed in normal temperature, utilizes the method for aeration or stirring to ventilate to blue algae slurry, to improve the oxygen content in blue algae slurry, and guarantee the aerobic region forming local in blue algae slurry, the subregional aerobic fermentation of forming portion;
(3), there is putrefactive phenomenons such as turning yellow, send out brown in blue algae slurry ventilation treatment after 10 days at normal temperatures, now added in the container holding tap water by the blue algae slurry after rotten and dilute;
(4) container holding tap water and rotten blue algae slurry mixed solution is placed in outdoor and opens wide environment, and carry out aeration or stirring;
(5) when normal temperature is the summer of 25-35 DEG C, aeration or stirring, after 10 days, hold in the container of tap water and rotten blue algae slurry mixed solution and can occur planktonic green algae advantage.
Scheme of the present invention, described in step (1), the total nitrogen concentration of blue algae slurry is at 200-500mg/L, and total phosphorus concentration is at 20-60mg/L.
Scheme of the present invention, the most high oxygen concentration in aerobic region described in step (2) is at more than 6mg/L, and the volume size in each aerobic region is indefinite, and the distribution density in aerobic region is 15-40/m
3.
In scheme of the present invention, in the container of storage blue algae slurry, rainwater can not be entered.
Scheme of the present invention, in step (3) with the total nitrogen of mixed solution of the blue algae slurry after tap water dilution and tap water, total phosphorus concentration respectively at 50-80mg/L, 4.5-9mg/L.
Scheme of the present invention, step carries out aeration or stirring in (4), to keep in mixed solution oxyty at more than 3mg/L.
The invention has the beneficial effects as follows:
1, under the general eutrophic overall background of current China water body, blue-green alga bloom easily obtains, and raw materials cost is low.
2, cultural method of the present invention does algae kind without the need to inoculating green alga in experimentation, and culture cycle is short, has greatly saved time and labour cost.
3, the chlorophyll-a concentration in the water body of the present invention after aeration is up to 600 more than μ g/L, and main green alga kind has Scenedesmus, Chlorella, egg capsule Trentepohlia etc., and the zooplankton or some fish and shrimp that can be the green alga that ingests provide high-quality food.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
embodiment 1
Obtain blue-green alga bloom in the July of high temperature from Lake Taihu, and utilize the part water in 200 object filter screen filtering blue-green alga blooms, make blue-green alga bloom be pulpous state, be transferred to by blue algae slurry subsequently in outdoor 100L white plastic bucket, the volume of blue algae slurry reaches about 70L.Now in bucket, the total nitrogen concentration of blue algae slurry is 320mg/L, and total phosphorus concentration is 40mg/L.Carry out Air Exposure to the blue algae slurry in bucket, aeration uses mode pneumatic pump connecting gas stone, places 2 gas stones in bucket altogether, 24 hours every days lasting aeration.
Aeration is after 3 days, and namely occur a large amount of foam in visible bucket, blue algae slurry turns yellow and is with a little brown, and occurs a little stink.Aeration about 10 days, rotting of blue algae slurry is more obvious, and blue algae slurry almost becomes tawny entirely, and the band significantly organic stink rotted.Now, rotten blue algae slurry is added to another one and hold in the white plastic bucket of tap water, after regulating the ratio of blue algae slurry and tap water, total nitrogen, the total phosphorus concentration of blue algae slurry and tap water mixed solution are respectively 63mg/L, 5.6mg/L, and cumulative volume is 60L.And by mode pneumatic pump connecting gas stone, lasting aeration being carried out to it, aeration can have green by water breakthrough colour band after about 4 days.Aeration after about 10 days water body become non-evergreen, getting its water examines under a microscope visible, now water body has been green alga advantage, and main dominant species has Chlorophyta Scenedesmus, Chlorella, egg capsule Trentepohlia etc., and the chlorophyll-a concentration of water body reaches 650 μ g/L.
embodiment 2
Obtain blue-green alga bloom in the July of high temperature from Chaohu, and utilize the part water in 200 object filter screen filtering blue-green alga blooms, make blue-green alga bloom be pulpous state, be transferred to by blue algae slurry subsequently in outdoor 100L white plastic bucket, the volume of blue algae slurry reaches about 70L.Now in bucket, the total nitrogen concentration of blue algae slurry is 500mg/L, and total phosphorus concentration is 60mg/L.Carry out Air Exposure to the blue algae slurry in bucket, aeration uses mode pneumatic pump connecting gas stone, places 2 gas stones in bucket altogether, 24 hours every days lasting aeration.
Aeration is after 3 days, and namely occur a large amount of foam in visible bucket, blue algae slurry turns yellow and is with a little brown, and occurs a little stink.Aeration is after 10 days, and rotting of blue algae slurry is more obvious, and blue algae slurry almost becomes tawny entirely, and the band significantly organic stink rotted.Now, rotten blue algae slurry is added to another one and hold in the white plastic bucket of tap water, after regulating the ratio of blue algae slurry and tap water, total nitrogen, the total phosphorus concentration of blue algae slurry and tap water mixed solution are respectively 80mg/L, 9mg/L, and cumulative volume is 60L.And by mode pneumatic pump connecting gas stone, lasting aeration being carried out to it, aeration gets final product water breakthrough colour cast after about 4 days green.Aeration after about 10 days water body become non-evergreen, getting its water examines under a microscope visible, now water body has been green alga advantage, main dominant species has Chlorophyta Scenedesmus, Chlorella, egg capsule Trentepohlia etc., especially wherein the ratio of Chlorophyta Scenedesmus cell quantity reaches about 80%, and the chlorophyll-a concentration of water body reaches 950 μ g/L.
embodiment 3
Obtain blue-green alga bloom in the August of high temperature from Taihu Lake, and utilize the part water in 200 object filter screen filtering blue-green alga blooms, make blue-green alga bloom be pulpous state, be transferred to by blue algae slurry subsequently in outdoor 100L white plastic bucket, the volume of blue algae slurry reaches about 70L.Now in bucket, the total nitrogen concentration of blue algae slurry is 200mg/L, and total phosphorus concentration is 20mg/L.Carry out Air Exposure to the blue algae slurry in bucket, aeration uses mode pneumatic pump connecting gas stone, places 2 gas stones in bucket altogether, 24 hours every days lasting aeration.
Aeration is after 3 days, and namely occur a large amount of foam in visible bucket, blue algae slurry turns yellow and is with a little brown, and occurs a little stink.Aeration is after 10 days, and rotting of blue algae slurry is more obvious, and blue algae slurry almost becomes tawny entirely, and the band significantly organic stink rotted.Now, rotten blue algae slurry is added to another one and hold in the white plastic bucket of tap water, after regulating the ratio of blue algae slurry and tap water, total nitrogen, the total phosphorus concentration of blue algae slurry and tap water mixed solution are respectively 50mg/L, 4.5mg/L, and cumulative volume is 60L.And by mode pneumatic pump connecting gas stone, lasting aeration being carried out to it, aeration can water breakthrough colour cast green after 5 days.Aeration after about 10 days water body become non-evergreen, getting its water examines under a microscope visible, now water body has been green alga advantage, and main dominant species has Chlorophyta Scenedesmus, Chlorella, egg capsule Trentepohlia, Pediastrum etc., and the chlorophyll-a concentration of water body reaches 600 μ g/L.
Claims (6)
1. utilize lake blue algae wawter bloom to cultivate a method for planktonic green algae, it is characterized in that, comprise the following steps:
(1) there is this season of blue-green alga bloom in lake, from lake, obtain blue-green alga bloom, and filtering is carried out to wherein moisture obtain blue-green alga bloom slurry and blue algae slurry with concentrated blue-green alga bloom;
(2) described blue algae slurry is transferred in container, under being placed in normal temperature, utilizes the method for aeration or stirring to ventilate to blue algae slurry, to improve the oxygen content in blue algae slurry, and guarantee the aerobic region forming local in blue algae slurry, the subregional aerobic fermentation of forming portion;
(3), there is putrefactive phenomenons such as turning yellow, send out brown in blue algae slurry ventilation treatment after 10 days at normal temperatures, now added in the container holding tap water by the blue algae slurry after rotten and dilute;
(4) container holding tap water and rotten blue algae slurry mixed solution is placed in outdoor and opens wide environment, and carry out aeration or stirring;
(5) when normal temperature is the summer of 25-35 DEG C, aeration or stirring, after 10 days, hold in the container of tap water and rotten blue algae slurry mixed solution and can occur planktonic green algae advantage.
2. a kind of method utilizing lake blue algae wawter bloom to cultivate planktonic green algae according to claim 1, it is characterized in that, described in step (1), the total nitrogen concentration of blue algae slurry is at 200-500mg/L, and total phosphorus concentration is at 20-60mg/L.
3. a kind of method utilizing lake blue algae wawter bloom to cultivate planktonic green algae according to claim 1, it is characterized in that, the most high oxygen concentration in aerobic region described in step (2) is at more than 6mg/L, and the volume size in each aerobic region is indefinite, and the distribution density in aerobic region is 15-40/m
3.
4. a kind of method utilizing lake blue algae wawter bloom to cultivate planktonic green algae according to claim 1, is characterized in that, can not enter rainwater in the container of storage blue algae slurry.
5. a kind of method utilizing lake blue algae wawter bloom to cultivate planktonic green algae according to claim 1, it is characterized in that, in step (3) with the total nitrogen of mixed solution of the blue algae slurry after tap water dilution and tap water, total phosphorus concentration respectively at 50-80mg/L, 4.5-9mg/L.
6. a kind of lake blue algae wawter bloom that utilizes according to claim 1 cultivates the method for planktonic green algae, and it is characterized in that, step carries out aeration or stirring in (4), to keep in mixed solution oxyty at more than 3mg/L.
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Cited By (6)
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| CN105543148A (en) * | 2016-02-04 | 2016-05-04 | 中国科学院南京地理与湖泊研究所 | Method for culturing Rhodopseudomonas photosynthetic bacteria by using cyanobacterial bloom |
| CN106248600A (en) * | 2016-09-05 | 2016-12-21 | 中国科学院南京地理与湖泊研究所 | Methods of Assessment for Water Entrophication based on chromophoric dissolved organic matter absorptance |
| CN106754385A (en) * | 2016-12-01 | 2017-05-31 | 中国科学院南京地理与湖泊研究所 | A kind of utilization blue-green alga bloom is the method for raw material cultivation Chlorella phytoplankton |
| CN110547237A (en) * | 2019-10-08 | 2019-12-10 | 广东海洋大学深圳研究院 | Method for preventing and controlling blue algae bloom in shrimp pond by using oocyst algae |
| CN111423985A (en) * | 2020-04-29 | 2020-07-17 | 中国水产科学研究院渔业机械仪器研究所 | Fresh water diatom cultivation method |
| CN111567451A (en) * | 2020-06-17 | 2020-08-25 | 河海大学 | Device for comparison experiment of zooplankton simulated ecological potential difference |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105543148A (en) * | 2016-02-04 | 2016-05-04 | 中国科学院南京地理与湖泊研究所 | Method for culturing Rhodopseudomonas photosynthetic bacteria by using cyanobacterial bloom |
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| CN106248600A (en) * | 2016-09-05 | 2016-12-21 | 中国科学院南京地理与湖泊研究所 | Methods of Assessment for Water Entrophication based on chromophoric dissolved organic matter absorptance |
| CN106248600B (en) * | 2016-09-05 | 2019-12-20 | 中国科学院南京地理与湖泊研究所 | Lake eutrophication evaluation method based on colored soluble organic matter absorption coefficient |
| CN106754385A (en) * | 2016-12-01 | 2017-05-31 | 中国科学院南京地理与湖泊研究所 | A kind of utilization blue-green alga bloom is the method for raw material cultivation Chlorella phytoplankton |
| CN106754385B (en) * | 2016-12-01 | 2020-05-05 | 中国科学院南京地理与湖泊研究所 | Method for cultivating chlorella phytoplankton by using cyanobacterial bloom as raw material |
| CN110547237A (en) * | 2019-10-08 | 2019-12-10 | 广东海洋大学深圳研究院 | Method for preventing and controlling blue algae bloom in shrimp pond by using oocyst algae |
| CN110547237B (en) * | 2019-10-08 | 2021-07-13 | 广东海洋大学深圳研究院 | Method for preventing and controlling blue algae bloom in shrimp pond by using oocyst algae |
| CN111423985A (en) * | 2020-04-29 | 2020-07-17 | 中国水产科学研究院渔业机械仪器研究所 | Fresh water diatom cultivation method |
| CN111423985B (en) * | 2020-04-29 | 2021-09-24 | 中国水产科学研究院渔业机械仪器研究所 | Fresh water diatom cultivation method |
| CN111567451A (en) * | 2020-06-17 | 2020-08-25 | 河海大学 | Device for comparison experiment of zooplankton simulated ecological potential difference |
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