CN115152688A

CN115152688A - Land-based circular pond recirculating aquaculture system and aquaculture method based on same

Info

Publication number: CN115152688A
Application number: CN202210966136.2A
Authority: CN
Inventors: 黎建斌; 杨琼; 杨学明; 江林源; 梁军能; 黎一键; 敖秋桅; 文露婷; 唐章生; 李大列; 李旻; 闭显达; 李满园
Original assignee: Guangxi Academy of Fishery Sciences
Current assignee: Guangxi Academy of Fishery Sciences
Priority date: 2022-08-12
Filing date: 2022-08-12
Publication date: 2022-10-11

Abstract

The invention relates to the technical field of aquaculture. The invention provides a land-based circular pond recirculating aquaculture system which comprises a circular aquaculture pond, a fish manure sedimentation pond, a tail water collecting pond, a primary sedimentation pond, a secondary sedimentation pond and a tertiary ecological pond, wherein the rest parts except the fish manure sedimentation pond are sequentially communicated through a pipeline to form a circulating loop. The invention also provides a fish culture method using the land-based circular pond recirculating aquaculture system, which mainly comprises the processes of water injection, probiotic inoculation, seed feeding, sectional feeding, water regulation, excrement discharge, circulation and the like. By adopting the culture system and the culture method, the treatment effect of the culture sewage and the tail water is improved, the problems of single culture variety and frequent diseases of culture objects are solved, the structure of the culture system is simplified, the energy consumption and the operation cost are reduced, and the healthy and rapid development of the industry is promoted.

Description

Land-based circular pond recirculating aquaculture system and aquaculture method based on same

Technical Field

The invention relates to the technical field of aquaculture, in particular to a land-based circular pond circulating water aquaculture system and a aquaculture method based on the system.

Background

The existing land-based circular pond recirculating aquaculture system in China has the disadvantages of complex structure, high energy consumption, high construction and operation cost, and only varieties with high added value can be cultured due to the complex structure, high energy consumption and high operation cost, so that the cultured varieties are relatively single. Moreover, the existing land-based circular pond circulating water aquaculture system has poor aquaculture sewage and tail water treatment effect, low aquaculture density, frequent diseases of aquaculture objects, lack of efficient aquaculture production technology, and can not realize the universality and universality similar to pond aquaculture, thereby restricting the healthy and rapid development of industry.

Disclosure of Invention

The invention aims to provide a land-based circular pond recirculating aquaculture system and an aquaculture method based on the system, and by using the land-based circular pond recirculating aquaculture system and the aquaculture method based on the system, the treatment effect of aquaculture sewage and tail water is improved, the problems of single aquaculture variety and frequent disease occurrence of aquaculture objects are solved, the energy consumption and the operation cost are reduced, and the healthy and rapid development of the industry is promoted.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides a land-based circular pond recirculating aquaculture system which specifically comprises a circular aquaculture pond, a fish manure sedimentation pond, a tail water collecting pond, a primary sedimentation pond, a secondary sedimentation pond and a tertiary ecological pond.

Preferably, a double-sewage-discharge chassis device is arranged at the bottom of the circular culture pond, a water outlet and a sewage outlet are arranged on the double-sewage-discharge chassis device, and the water outlet is communicated with the tail water collecting pond through a water outlet pipeline; the sewage outlet is communicated with the fish manure sedimentation tank through a sewage pipeline; the fish manure sedimentation tank is provided with a tail water outlet and a manure outlet; and the tail water outlet is communicated with the tail water collecting tank through a pipeline. In the invention, the circular culture pond, the tail water collecting pond, the primary sedimentation pond, the secondary sedimentation pond and the tertiary ecological pond are sequentially communicated through pipelines to form a circulation loop.

Preferably, the circular culture pond is funnel-shaped, and the bottom of the circular culture pond downwards inclines towards the center by an angle of 5-25 degrees; the top end of the side surface of the circular culture pond is provided with a water inlet, the side surface of the circular culture pond is provided with a water pusher, and the central position of the bottom of the circular culture pond is provided with the double-drain chassis device; and an oxygenation device and an intelligent monitoring device are also arranged in the circular culture pond.

Preferably, the upper part of the fish manure sedimentation tank is hollow cylindrical, the lower part of the fish manure sedimentation tank is hollow conical, and the height position of the top of the fish manure sedimentation tank is lower than the bottom of the circular culture tank; the top of the tail water collecting pool is lower than the bottom of the circular culture pool.

Preferably, the heights of the bottoms of the first-stage sedimentation tank, the second-stage sedimentation tank and the third-stage ecological tank are sequentially reduced; and a circulating device is arranged on a pipeline between the three-stage ecological pond and the circular culture pond.

The invention also provides a fish culture method by utilizing the land-based circular pond recirculating aquaculture system, which specifically comprises the following steps:

(1) Injecting the filtered clean aquaculture water into a round aquaculture pond, and then applying a probiotic agent to the aquaculture water;

(2) And putting seedlings into the circular culture pond, adopting a sectional feeding mode, and carrying out water transfer and excrement discharge treatment on the circular culture pond during culture.

Preferably, the probiotic agent is one or two of rhodopseudomonas palustris agent, bacillus subtilis agent and lactobacillus plantarum agent.

Preferably, the feeding formula comprises daily feeding, clostridium butyricum feeding and wuhuang powder feeding; the daily feeding is feeding of an expanded compound feed, the feeding frequency is 1-4 times/d, and the feeding amount of each time is 1-5% of the mass of the fish body; the frequency of feeding the clostridium butyricum is continuously fed for 2-4 days every 5-10 days, and the feeding amount of the clostridium butyricum accounts for 2-8 per thousand in mass percent of the feeding amount of the puffed compound feed each time; the Wuhuang powder is fed at a high-temperature culture period or a disease development period, the frequency of feeding the Wuhuang powder is continuously fed for 2-4d every 3d-10d, and the feeding amount of the Wuhuang powder accounts for 2-8 per mill of the feeding amount of the puffed compound feed every time in percentage by mass.

Preferably, the water regulation comprises water regulation in the early stage of cultivation and water regulation in the middle and later stages of cultivation. The water regulation in the early stage of cultivation is to apply the probiotic bacteria agent for 1 time every day after seedlings enter a pond, wherein the application amount is 0.2-35 mg/L every time, and the probiotic bacteria agent is continuously sprinkled for 3-5 days. And after the seedlings are planted in the pond for 20-40 days, the water regulation in the middle and later stages of the cultivation is to apply hydrogen potassium persulfate compound salt tablets in the pond, and apply the probiotic bacteria agent after 8-15 hours, wherein the application frequency of the hydrogen potassium persulfate compound salt tablets is once per 2-3 days, the application amount per time is 0.5-2.0mg/L, and the application amount per time of the probiotic bacteria agent is 0.3-80 mg/L.

Preferably, 1 defecation is carried out after 0.1-2.5 h of feeding each time, and the defecation time is 20-70 s each time.

Preferably, the culture water body is kept circulating for 24 h/day, and the aerator runs for 24 h/day.

By adopting the technical scheme, the invention has the following beneficial effects:

1. in the invention, the collection rate of solid excrement and residual bait is more than 90 percent through the teacup effect in the pool and the solid-liquid separation outside the pool; the treated sewage and tail water are deeply purified by a primary sedimentation tank, a secondary sedimentation tank and a tertiary ecological tank to efficiently remove ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and PO in the water ₄ P, and the like, and the recycling rate of the culture tail water reaches 90 percent. Meanwhile, the culture system of the invention abandons the devices such as a rotary drum micro-filter, a carbon dioxide stripper, a protein separator, a dirt collector and the like used in the existing culture water treatment. Therefore, the culture system provided by the invention can obviously improve the treatment effect of culture sewage and tail water, simplify the system structure and reduce the operation cost and energy consumption.

2. According to the invention, clostridium butyricum and tabasheer powder with obvious antibacterial activity are screened out, and a puffed compound feed, clostridium butyricum and tabasheer powder sectional type feeding method is developed, so that the disease immunity and disease resistance of a cultured object can be obviously improved, the problem of frequent disease of the cultured object is effectively solved, and the edible safety of cultured products is ensured.

3. The invention constructs a water quality regulation and control technology which takes operations of culture pond treatment, culture water treatment, probiotic inoculation, water regulation in the early stage of culture, water regulation in the middle and later stages of culture and the like as main contents, eliminates the damage of harmful substances in water to culture objects on the basis of effectively improving the culture water quality environment, and reduces the occurrence of diseases and other problems of the culture objects.

The technical scheme of the invention better solves the problems of water quality and oxygenation, so the problem of low culture density is improved, and because the structure of a culture system is simplified and the operation cost and the energy consumption are reduced, the culture system can not only be limited to varieties with high culture additional value, but also can select varieties with low additional value, so the problem of relatively single culture variety is improved, and the economic and ecological benefits are further ensured. Meanwhile, the method realizes low-carbon, high-efficiency and zero-pollution discharge, resource utilization and universality of aquaculture tail water, and has great market popularization value.

Drawings

FIG. 1 is a construction diagram of the land-based circular pond recirculating aquaculture system (in FIG. 1:1 circular aquaculture pond; 2 fish manure sedimentation pond; 3 tail water collection pond; 4 primary sedimentation pond; 5 secondary sedimentation pond; 6 tertiary ecological pond; 7 circulating pump);

FIG. 2 is a structure of a circular culture pond in a land-based circular pond recirculating aquaculture system;

FIG. 3 shows the conventional land-based circulating water aquaculture system of the base described in comparative example 1 (FIG. 2: 1 is a culture tank, 2 is a sewage lifting device, 3 is a water-sewage separator, 4 is a brush treatment tank, 5 is a microorganism treatment tank, 6 is an aquatic plant treatment tank, 7 is a water purification and disinfection tank, 8 is a reflux lifting device, and 9 is a tail water biochemical treatment tank).

Detailed Description

The invention provides a land-based circular pond recirculating aquaculture system which specifically comprises a circular aquaculture pond, a fish manure sedimentation pond, a tail water collecting pond, a primary sedimentation pond, a secondary sedimentation pond and a tertiary ecological pond. The area ratio of each functional area of the circular culture pond, the fish manure sedimentation pond, the tail water collection pond, the primary sedimentation pond, the secondary sedimentation pond and the tertiary ecological pond is (1-6): 1.

In the invention, the construction material of the circular culture pond is preferably galvanized steel plate and high-strength PVC canvas (see figure 2), and the diameter of the circular culture pond is preferably 6m-10m, more preferably 7m-9m, and even more preferably 8m; the depth of the circular culture pond is 1.5m-2.5m, more preferably 1.8m-2.2m, and still more preferably 2m. The circular culture ponds can be multiple, and the interval between the circular culture ponds is 1m-2m, and further preferably 1.5m. The round culture pond is in a funnel shape, the bottom of the round culture pond is inclined downwards towards the center by an angle of 5-25 degrees, more preferably 10-20 degrees, and still more preferably 15 degrees. The funnel-type bottom surface of the circular culture pond is convenient for solid waste to be concentrated and deposited at the center of the bottom of the pond under the tea cup effect.

In the invention, the circular culture pond is provided with a water inlet, a water pusher and a double-drain chassis device. The water inlet is arranged at the top end of the side surface of the circular culture pond, and the water inlet circulation volume is 10m ³ /h-20m ³ H is used as the reference value. The number of the water pushers is not particularly limited, in a specific embodiment of the invention, the number of the water pushers is preferably 2, the water pushers are symmetrically arranged on the side surface of the circular culture pond and are 1/2 or 1/3 away from the water surface, and the water body in the pond is kept in a rotating state for 24 hours; the rotating speed of the aquaculture water body is controlled to be 1m/s-2m/s, preferably 1.5m/s at the early stage of aquaculture and 2m/s at the later stage of aquaculture. Double blowdown chassis device sets up and puts at bottom of the pool central point, double blowdown chassis device is provided with outlet and drain, fish excrement, tail water separately discharge mode. Wherein, the outlet passes through drainage pipe and tail water collecting pit intercommunication, the drain passes through sewage pipes and fish manure sedimentation tank intercommunication. In the invention, fish manure is directly discharged into a fish manure sedimentation tank through a sewage discharge pipeline, a control switch is arranged in the sewage discharge pipeline communicated with a sewage discharge outlet, and the diameter of the pipe orifice of the sewage discharge pipeline is preferably 20cm-30cm; the tail water discharge pipe is positioned in the center of the circular pool and is 20cm-30cm lower than the pool surface, the diameter of the pipe orifice is preferably 30cm-40cm, an escape-proof net is preferably arranged at the pipe orifice of the tail water discharge pipe, and tail water is directly discharged into the tail water collecting pool through the discharge pipe.

In the invention, the circular culture pond is also internally provided with an oxygen increasing device and an intelligent monitoring device, the oxygen increasing device is provided with a fan according to the volume of the culture pond water, and the air inflow is not less than 30m ³ And h, the air inlet pipe is connected with an exhaust valve and a PVC/PPR pipe, and a dissolved oxygen probe is matched in the pool to ensure that the dissolved oxygen of the water body in the pool is 5mg/L-8mg/L. The intelligent monitoring equipment preferably comprises online water quality monitoring equipment.

In the invention, the fish manure sedimentation tank adopts a brick cement plastering structure, the upper part is hollow cylindrical, the lower part is hollow conical, the diameter of the upper part is 4m-12m, the depth is 2m-3m, and the depth of the lower part is 1m-2m. The top of the fish manure sedimentation tank is 1m-2m lower than the bottom of the culture tank body, and the optimal range is 1.5m. In the invention, the fish manure sedimentation tank is also provided with a tail water outlet and a manure outlet, the tail water outlet is communicated with a tail water collecting tank through a pipeline, surface sewage precipitated in the fish manure sedimentation tank can pass through the tail water outlet to carry out the tail water collecting tank, and the surface sewage and tail water are deeply purified and then return to the culture tank for recycling; the excrement discharge port is used for discharging the residual fish excrement precipitated in the fish excrement sedimentation tank, and the discharged fish excrement is subjected to solid-liquid separation or natural drying and then is applied with a microbial fermentation agent to produce a biological fertilizer for crop planting.

In the invention, the tail water collecting tank is plastered by using brick cement, and the depth of the tank is 1-2 m, preferably 1.5m; the top of the tail water collecting tank is 1m-2m, preferably 1.5m lower than the bottom of the culture tank body.

In the invention, the first-stage sedimentation tank, the second-stage sedimentation tank and the third-stage ecological tank are arranged in a step height mode, the distance is not specially limited, the heights of the bottoms of the three tanks are sequentially reduced and sequentially decreased by 0.2-0.4 m, and preferably 0.3m.

In the invention, the first-stage sedimentation tank and the second-stage sedimentation tank are plastered by brick cement, the third-stage ecological tank is a soil pond, the depth of the three tanks is 1m-2m, preferably 1.5m, and the area ratio is 1. The primary sedimentation tank and the secondary sedimentation tank are fully hung with brushes, and the water surface is fully distributed with water hyacinth to block large-particle-size particles and absorb nitrogen and phosphorus in water, so that the sedimentation process is completed. The three-stage ecological pond is in a fish and vegetable co-culture mode, filter-feeding fishes are released and cultured, vegetables are planted on the water surface and placed on floating plates, the vegetables are planted, the released filter-feeding fishes are preferably silver carps and bighead carps, and the releasing density is preferably 4-6 fish/m ³ The stocking proportion is preferably 3:1 with the specification of more than 1 kg/tail; the area of the vegetable planting floating plate accounts for 1/(2-3), preferably 1/2 of the area of the three-level ecological pool; the planted vegetables are preferably water spinach, and deep purification is completed.

In the invention, the circular culture pond, the tail water collecting pond, the primary sedimentation pond, the secondary sedimentation pond and the tertiary ecological pond are sequentially communicated through pipelines to form a circulation loop. A circulating device is arranged on a pipeline between the three-stage ecological pond and the circular culture pond, the circulating device is mainly a circulating water pump, the power is set according to the water volume of the circular culture pond, and the circulating flow is 10m ³ /h-20m ³ And/h, and 24h are kept in circulation.

The invention also provides a culture method for culturing fish by using the land-based circular pond recirculating aquaculture system, which specifically comprises the following steps:

In the invention, the circular culture pond is pretreated before use, wherein the pretreatment comprises draining pond water, flushing with a high-pressure pump, removing sludge at the bottom of the pond, and airing or solarizing the bottom of the pond for 3-4 days. In the invention, the breeding water is preferably well water, lake water, river water and reservoir water; the filtering operation adopts a filter screen, the aperture of the filter screen is preferably 80 meshes, and the filtering function is to remove wild trash fish, enemy organisms, large-particle-size suspended matters and floating matters in the culture water.

In the present application, after the filtered clean aquaculture water is injected into the circular aquaculture pond, 0.2 to 2% of NaCl is preferably added, more preferably 0.5 to 1.5% of NaCl, and still more preferably 1% of NaCl. The water injection depth of the culture pond is 1.5m-2.5m, more preferably 1.7m-1.9m, and still more preferably 1.8m. The purpose of adding NaCl in the invention is to relieve the toxicity and osmotic pressure of nitrite in the aquaculture water.

In the invention, after NaCl is added and before 5d-7d of offspring seed is put in the morning on a sunny day, 1 time of probiotic agent is applied to the culture water, and the application amount is 0.3mg/L-15mg/L. The probiotic agent is one or two of rhodopseudomonas palustris agent, bacillus subtilis agent and lactobacillus plantarum agent, and the probiotic agent can be preferably 15-60 mg/L of rhodopseudomonas palustris agent; preferably 0.3mg/L-1mg/L of bacillus subtilis microbial inoculum; preferably 15mg/L-60mg/L of lactobacillus plantarum microbial inoculum; can be preferably 15mg/L-60mg/L of lactobacillus plantarum microbial inoculum and 15mg/L-60mg/L of rhodopseudomonas palustris microbial inoculum; the bacillus subtilis preparation can also be preferably 0.15mg/L-0.5mg/L and 7.5mg/L-30mg/L of rhodopseudomonas palustris microbial inoculum. In the invention, the formulation of the rhodopseudomonas palustris microbial inoculum is preferably aqueous, and the effective viable count of the rhodopseudomonas palustris is preferably more than or equal to 8.0 multiplied by 10 ⁸ CFU/mL; the formulation of the bacillus subtilis microbial inoculum is preferably powder, and the effective viable count of the bacillus subtilis is preferably more than or equal to 100 multiplied by 10 ⁸ CFU/g; the formulation of the lactobacillus plantarum microbial inoculum is preferably an aqueous solution, and the effective viable count of the lactobacillus plantarum is preferably more than or equal to 5.0 multiplied by 10 ⁸ CFU/mL. The probiotic agent is added to thoroughly decompose toxic substances in the water body of the system, inhibit pathogenic bacteria, convert the toxic substances into nutritive salt required by algae, eliminate hidden dangers, enable the whole system to form an ecological environment mainly comprising beneficial microbial flora and lay a good foundation for successful cultivation.

In the invention, after the probiotic agent is applied, water testing operation is carried out before offspring seed putting, a system needs to run in a test mode before offspring seed putting, and whether water inlet and outlet pipelines, a water pump, an air pump and the like normally run is tested; taking the original pond water of the culture pond for water testing, wherein the water testing time is not less than 24h, and if the fish moves normally, the fish can be released.

In the invention, the breeding seeds before putting are disinfected after the water testing operation. The disinfection mode is to soak and wash in 1-3% NaCL water solution for 5-10 min. The cultured seedlings are legal in source and qualified in quarantine, and are required to be normal in body shape, free of diseases and injuries and complete in scales and fin rays. The variety of the cultured seedlings is a fish variety which can eat puffed compound feed and is suitable for high-density intensive culture, the bait coefficient is generally below 1.2, and the variety is suitable for the variety with higher economic value. The variety of the cultured fries is preferably one or more of micropterus salmoides, channa maculata, pseudobagrus fulvidraco and spotted tail. In the invention, the putting specification of the breeding fries is preferably 20g-50g, and the stocking density is preferably 60 tails/m according to the oxygen increasing capacity and the water quality control capacity ³ 120 tails/m ³ Further, it is preferably 80 tails/m ³ -100 tails/m ³ 。

In the present invention, the staged feeding includes daily feeding, clostridium butyricum feeding, and wuhuang flour feeding. The daily feeding is feeding of an expanded compound feed, the feeding frequency is 1-4 times/d, and the preferable frequency is 2-3 times/d; the feeding amount of each time is 1-5% of the fish body, and the preferable feeding amount is 2-5%. The feeding expanded compound feed is particularly preferred, the feeding frequency is 3 times/d in the early stage of cultivation, namely 30 days after seedlings enter a pond, and the feeding amount is 4 percent of the mass of fish bodies in the morning, at noon and at night generally; the feeding frequency is 2 times/d in the middle and later culture periods, namely 30 days after the seedlings are put into the pond, and the feeding amount of each time is 2 percent of the mass of the fish body in the morning and evening. In the invention, when the clostridium butyricum is fed for ordinary prevention, the feeding frequency is 1 time every 5d-10d, more preferably 1 time every 7d-9d, and still more preferably 1 time every 8 d; 2-4d is continuously used for each feeding, and the further preference is 3d; the mass percentage of the feeding amount of the clostridium butyricum in each feeding amount of the puffed compound feed is 2-8 per thousand, more preferably 4-6 per thousand, and even more preferably 5 per thousand. In the invention, the Wuhuang powder is fed at the high-temperature period or the disease period of cultivation, the feeding frequency is 1 time every 3d-10d, the feeding frequency is further preferably 1 time every 5d-7d, and the feeding frequency is further preferably 1 time every 6 d; 2-4d is continuously used for each feeding, and the further preference is 3d; the feeding amount of the five-yellow powder in each time accounts for 2-8 per thousand, more preferably 4-6 per thousand, and even more preferably 5 per thousand of the feeding amount of the puffed compound feed.

In the invention, the preparation form of the clostridium butyricum microbial inoculum is preferably aqueous solution, and the effective viable count of clostridium butyricum is preferably more than or equal to 5.0 multiplied by 10 ⁸ CFU/mL; the main components of the five-yellow powder are preferably scutellaria baicalensis, golden cypress, rheum officinale, coptis chinensis and astragalus membranaceus. The clostridium butyricum has the effects of promoting the proliferation of beneficial microorganisms in the intestinal tract, inhibiting the growth of other harmful bacteria and putrefying bacteria, reducing the occurrence of infectious diseases in the intestinal tract and improving the immunity of organisms, and particularly, the main metabolite butyric acid is a main substance for repairing and regenerating epithelial cells in the intestinal tract, can promote the development of the intestinal tract of livestock and poultry, strengthen various functions of the livestock and poultry, enhance the immunity and disease resistance of the livestock and poultry, provide main energy for colon mucosal cells and provide important guarantee for the proliferation and maturation of the intestinal tract cells. The five-yellow powder has the functions ofIs used for clearing away heat and toxic materials, and is mainly used for treating bacterial diseases of aquatic animals caused by pseudomonas, vibrio, aeromonas hydrophila and edwardsiella.

In the invention, the water regulation treatment is carried out on the circular culture pond during the culture period, and the water regulation comprises the water regulation at the early stage of the culture and the water regulation at the middle and later stages of the culture. The water adjustment in the early stage of cultivation is to apply the probiotic bacteria agent for 1 time every day after seedlings are put into a pond, wherein the application amount is 0.2-35 mg/L, and further preferably 0.5-30 mg/L, and the probiotic bacteria agent is continuously sprinkled for 3-5 days until the water transparency is 0.5-0.6 m. The probiotic agent is used for absorbing and utilizing macromolecular organic matters and micromolecular inorganic matters, balancing the propagation of floating microalgae, absorbing harmful factors such as ammonia nitrogen, nitrite and hydrogen sulfide in the aquaculture water body and purifying the water quality.

In the invention, water regulation in the middle and later periods of cultivation is carried out after the seedlings are planted in the pond for 20-40 days, more preferably for 25-35 days, and still more preferably for 30 days; the water regulation operation in the middle and later periods of the cultivation is to apply the oxone complex salt tablets in the cultivation pond and apply the probiotic bacteria agent after 8-15 h, more preferably 10-14 h, and even more preferably 12h. The frequency of the administration of the oxone complex salt tablets is 1 time per 2d-3d, and is further preferably 1 time per 3d; the administration amount of the potassium hydrogen persulfate compound salt tablet is 0.5-2.0mg/L, and the more preferable is 1mg/L. The administration amount of the probiotic agent is 0.3mg/L-80mg/L each time, and further preferably 1mg/L-60mg/L. In the invention, the main components of the potassium hydrogen persulfate composite salt tablet are composite potassium hydrogen persulfate powder (KMPS + catalytic system), peroxide for decomposing algae toxins and toxic organic substances (SPS + catalytic system), and biological floc forming agent (polysaccharide + porous material + polyphosphate); the content specification of the potassium monopersulfate composite salt tablet is calculated by that the content of the composite potassium monopersulfate powder is more than or equal to 25 percent, and the content of the algal toxin-decomposing peroxide is more than or equal to 5 percent. The application of the potassium hydrogen persulfate composite salt tablet has the effects of effectively inhibiting the growth of pathogenic bacteria, degrading algae toxins in the water body, oxidizing ferrous iron in the water body into ferric iron, converting the ferrous manganese into manganese dioxide, converting nitrite into nitrate, converting sulfides such as hydrogen sulfide into sulfate, eliminating the harm of the substances to aquatic animals, improving the phenomena of stickiness, blackening and smelliness of the water body, and enabling detoxified residual baits, excrement, dead algae particles and the like to form suspended organic debris.

In the invention, the 'potassium hydrogen persulfate composite salt tablet and probiotics' are used in a matching way, and the effect is that the potassium hydrogen persulfate composite salt tablet is degraded to provide beneficial elements such as potassium, sodium, phosphorus, magnesium and the like for a water body, the growth and the propagation of the probiotics can be increased after the probiotics are supplemented, biological floccules with stronger water purification capacity are formed and suspended on the water surface, and the biological floccules are discharged from a tail water discharge pipe orifice under the rotation and circulation of the water body to ensure that ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and PO are discharged from the tail water discharge pipe orifice ₄ The long time such as-P is in normal level, which is a composite water treatment technology of 'green bacteriostasis, advanced oxidation detoxification and probiotics'.

In the invention, the feeding is followed by a defecation treatment, wherein 1 defecation is carried out after each feeding for 0.1-2.5 h, and the further optimization is 0.5-1.5 h; the defecation time is 20s-70s, and more preferably 30s-60s.

In the invention, water is not changed during the culture period, and only new water with corresponding percentage content is supplemented from the outside of the system; keeping circulation of the aquaculture water body for 24 h/day; the aerator runs 24 h/day and keeps the dissolved oxygen at 5mg/L-8mg/L all day.

In the invention, the final harvest is captured timely according to climate, specification, market price and water body condition.

The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.

Description of the Experimental materials

The rhodopseudomonas palustris is purchased from Nanning Haiyi Biotech limited, and has the product name: mixed feed additive (rhodopseudomonas palustris), product standard: Q/NNHY 02-2021;

the bacillus subtilis is purchased from south China sea easy biotechnology limited company with the product name: mixed feed additive (bacillus subtilis), product standard: Q/NNHY 01-2021;

the lactobacillus plantarum is purchased from south Ninghai Yiyi Biotech Co., ltd, and has a product name: mixed feed additive (lactobacillus plantarum), product standard: Q/NNHY 03-2021;

the clostridium butyricum is purchased from south china haiyi biotechnology limited, and has the product name: mixed feed additive (clostridium butyricum), product standard: Q/NNHY 04-2021;

the five-yellow powder is purchased from Guangxi general big animal health products Co., ltd, and the product name is as follows: wuhuang powder, production license number: (2014) veterinary drug production license number 20007;

the potassium hydrogen persulfate composite salt tablet is purchased from Shanghai Taiyuan Biotech GmbH, product name: probiotic bacteria-free tablet (potassium hydrogen persulfate composite salt tablet), product standard: Q31/0117000095C014-2016.

Example 1

A land-based circular pond recirculating aquaculture system, includes that each functional area proportion is 4.

4 circular culture ponds (number 1) ^# 、2 ^# 、3 ^# 、4 ^# ) The pool is built by galvanized steel plates and high-strength PVC canvas, the diameter of each pool is 8m, the depth of each pool is 2m, and the bottom of each pool is inclined downwards towards the center by 15 degrees; total volume: 300m ³ And the distance between the round ponds is 1.5m. The top end of the side surface of the circular culture pond is provided with a water inlet, and the water inlet circulation volume is 15m ³ H; two water pushers are symmetrically arranged on the side surface of the circular culture pond 1/3 away from the water surface of the circular culture pond, the water body in the culture pond keeps a rotating state for 24 hours, the rotating speed of the water body in the pond in the early stage of culture is 1.5m/s, and the rotating speed of the water body in the pond in the later stage of culture is 2m/s; a double-drain chassis device is arranged at the center of the bottom of the round culture pond. This double blowdown chassis device has a mouth of pipe diameter to be 25 cm's drain and a mouth of pipe diameter to be 35 cm's outlet, the drain is through the sewage pipes who is equipped with control switch and fish excrement sedimentation tank intercommunication, drain department is equipped with prevents escaping the net to through drainage pipe and tail water collecting pit intercommunication, drainage pipe is less than pool face 25cm.

The tail water collecting tank is plastered by brick cement, the tank depth is 1.5m, and the tank top is lower than the bottom of the circular culture tank body by 1.5m.

The fish manure sedimentation tank adopts the plastering structure of brick cement, and the upper portion is the interior empty cylinder type of diameter 5m, degree of depth 2m, and the lower part is the interior empty circular cone type that the degree of depth is 1m, volume: 75m ³ . The height position of the top of the pond is 1.5m lower than the bottom of the circular culture pond, and the pond is also provided with a tail water outlet and a feces outlet. After the sludge is precipitated in the fish manure sedimentation tank, the surface layer sewage is directly discharged into the tail water collection tank through a tail water discharge port and a drainage pipeline, and after the residual fish manure is discharged through a manure discharge port, solid-liquid separation or natural drying is carried out, and then a microbial starter is applied to produce a biological fertilizer for crop planting.

One-level sedimentation tank, second grade sedimentation tank, tertiary ecological pond, three pond are ladder height range, and the interval does not have special restriction, and three pond bottom of the pool height is decreased progressively 0.3m after to in the past in proper order. Wherein the first-stage sedimentation tank and the second-stage sedimentation tank are plastered by brick cement, the third-stage ecological pond is a soil pond, the depth of the three ponds is 1.5m, and the volume of the three ponds is 75m ³ 、75m ³ 、150m ³ (ii) a The third-level ecological pond is in a fish and vegetable co-culture mode, the total culture of 36 silver carps and bighead carps is realized, the culture proportion is 3:1, and the specification is more than 1 kg/tail; the area of the water spinach planting floating plate accounts for 1/2 of the area of the three-level ecological pond.

And a circulating device capable of keeping circulation for 24 hours is arranged on a pipeline between the three-level ecological pond and the circular culture pond, and an oxygen increasing device and an intelligent monitoring device are also arranged in the circular culture pond. The circulating equipment adopts a large-flow low-lift circulating water pump of Bo Shang Zhiyu: model IC-45B, power 3.75kw, lift 12m, standard water volume 60m ³ H, ensuring the water circulation volume of each culture pond to be 15m ³ H is used as the reference value. The oxygenation equipment adopts a Xinruituo Roots type oxygenation machine: power 3kw, air flow 150m ³ H, ensuring that the air input of each culture pond is not less than 30m ³ And h, the air inlet pipe is connected with an exhaust valve and a PVC pipe, and a dissolved oxygen probe is arranged in the tank, so that the dissolved oxygen of the water body in the tank is ensured to be 6mg/L.

Circular breed pond, tail water collecting pit, one-level sedimentation tank, second grade sedimentation tank and tertiary ecological pond communicate through the pipeline in proper order, form circulation circuit.

Example 2

A culture method for culturing fish by using the land-based circular pond recirculating aquaculture system comprises the following steps:

(1) And (5) treating the culture pond. Draining the pond water, washing with a high-pressure pump, removing sludge at the bottom of the pond, and airing or solarizing the bottom of the pond for 3d to obtain the clean pond for breeding.

(2) And (5) treating culture water. River water filtered by a 80-mesh screen is selected as culture water, injected into a circular culture pond with the water injection depth of 1.8m, and then 1 thousandth of NaCl is added into the circular culture pond.

(3) And (3) attaching and inoculating the probiotic agent. Applying 30mg/L rhodopseudomonas palustris microbial inoculum (the effective viable count is preferably more than or equal to 8.0 multiplied by 10) to a round culture pond for 1 time in the morning on a sunny day 6 days before the culture seedlings are put in ⁸ CFU/mL) and 0.5mg/L of Bacillus subtilis microbial inoculum (the effective viable count is preferably more than or equal to 100 multiplied by 10) ⁸ CFU/g) in an amount of 9mg/L.

(4) And selecting breeding seedlings. Selecting cultured fries which are legal in source and qualified in quarantine, normal in body form, free of diseases and injuries and complete in scales and fin lines, and putting the cultured fries into the micropterus salmoides which can eat puffed compound feed and are suitable for high-density intensive culture, wherein the bait coefficient is 1.0.

(5) And (6) testing water. Before putting the cultured seedlings, the system needs to be operated in a trial mode, and whether water inlet and outlet pipelines, a water pump, an air pump and the like operate normally is tested; and (4) taking the original pond water of the circular culture pond for water testing for 24 hours, and stocking the fish seeds if the fish seeds move normally.

(6) And (5) sterilizing the cultured seedlings. Before putting in, soaking and washing the cultured seedlings for 7min by using 2% NaCl.

(7) And putting and culturing the seedlings. The breeding specification of micropterus salmoides fry is 50 g/tail, and the breeding density is 80 tail/m ³ 。

(8) During the cultivation period, sectional type feeding puffed compound feed, clostridium butyricum and Wuhuang powder are adopted, and water adjustment in the early stage of cultivation, water adjustment in the middle and later stages of cultivation and excrement discharge treatment are carried out on the circular cultivation pond.

Feeding with the puffed compound feed, wherein the feeding frequency is 3 times/d within 30 days after the seedlings are put into a pond, and the feeding amount is 4 percent of the fish body mass in morning, noon and evening generally; feeding the seedlings 30 days after the seedlings enter the pond for 2 times/d, wherein the feeding amount of each time is 2 percent of the mass of the fish body in the morning and evening; in usual prevention, the feeding frequency of the clostridium butyricum is 1 time every 8 days, the clostridium butyricum is continuously fed for 1 time for 3 days, and the feeding amount of the clostridium butyricum accounts for 5 per thousand by mass percent of the feeding amount of the puffed compound feed; in the high-temperature period or disease-onset period of the cultivation, the five-yellow powder is fed for 1 time every 6 days, the five-yellow powder is continuously fed for 3 days for 1 time, and the feeding amount of the five-yellow powder accounts for 5 per mill of the feeding amount of the puffed compound feed each time.

After the seedlings are put into the pond, 30mg/L of rhodopseudomonas palustris microbial inoculum (the effective viable count is preferably more than or equal to 8.0 multiplied by 10) is applied to the round culture pond for 1 time every day ⁸ CFU/mL) and 0.5mg/L of Bacillus subtilis microbial inoculum (the effective viable count is preferably more than or equal to 100 multiplied by 10) ⁸ CFU/g) is 10mg/L, and 4 days are continuously splashed until the transparency of the water body is 0.5m. And (3) after the seedlings enter the pond for 30 days, applying hydrogen potassium persulfate compound salt tablets in the culture pond, and applying the probiotic bacteria agent after 12 hours, wherein the hydrogen potassium persulfate compound salt tablets are applied for 1 time every 3 days, the application amount of each time is 1mg/L, and the application amount of each time of the probiotic bacteria agent is 1mg/L.

And (3) performing 1-time excrement discharge after feeding for 1h every time, wherein the excrement discharge time is preferably 60s every time, and the culture water body is kept circulating for 24 h. Water is not changed during the culture period, the circulation utilization rate of culture tail water reaches 90%, and only 10% of new water is supplemented from the outside of the system; the aerator runs for 24 hours, and dissolved oxygen is kept at 6mg/L all day.

(9) And (6) harvesting. Timely capturing according to the climate, specification, market price and water body condition.

Example 3

Based on the study of the bacteriostatic activity of clostridium butyricum and wuhuang powder of the present invention in examples 1-2.

In 13 days 4 months after 2020, nocardia, edwardsiella tarda and hirsutella albiflora pathogenic strains separated from diseased micropterus salmoides are taken as test strains, and the antibacterial activity of 5 compound Chinese herbal medicine aqueous extracts and clostridium butyricum on nocardia, edwardsiella tarda and hirsutella albiflora pathogenic strains common to micropterus salmoides is measured by an Oxford cup method, and the antibacterial activity is shown in Table 1.

TABLE 1 antibacterial activity of Clostridium butyricum and 5 kinds of compound Chinese herbal medicines

It can be seen that 5 compound Chinese herbal medicine aqueous extracts of the three-yellow powder, the five-yellow powder, the Chinese gall powder, the Chinese globeflower powder and the isatis root have obvious in-vitro antibacterial activity on nocardia of micropterus salmoides, edwardsiella tarda and rhizoctonia solani, wherein the in-vitro antibacterial effect of the five-yellow powder is most obvious; the clostridium butyricum also has strong bacteriostatic activity on three common pathogenic bacteria of the micropterus salmoides. Therefore, the clostridium butyricum and the five-yellow powder have good implementation prospects.

Example 4

Based on the examples 1-2, the influence of different feeding methods of feeding the expanded compound feed, the clostridium butyricum and the yellow powder on the disease resistance of the micropterus salmoides is researched.

Selecting 600 micropterus salmoides fries in 25 days 6 months in 2020, setting 4 treatment groups with the initial mass of 50 +/-2.6 g/tail, repeating 3 treatment groups for each treatment group and 50 tail for each treatment group, adopting open-type running water culture, and after feeding for 8 weeks, randomly extracting 20 fishes from each group to perform a nocardia virus challenge test. Bacterial liquid concentration 10 ⁶ cfu/ml, injection dose 0.2 ml/tail, continuous observation for 2 weeks. The experimental design is shown in Table 2 and the results are shown in Table 3.

TABLE 2 different test designs

TABLE 3 Effect of different feeding methods on results

Note: the difference was significant when the letters were not the same between the data in the same column (P < 0.05).

As can be seen from Table 3, after 8 weeks of feeding, 4 groups all died after artificial nocardia infection, and the death of Micropterus salmoides was identified and found to be caused by nocardia infection, 1 ^# Control group mortality 77%,2 ^# Mortality rate 69%,3 ^# Mortality rate 64%,4 ^# The mortality rate is 52 percent, and the ratio of the test group to the control group is remarkably different (P)<0.05)，2 ^# 、3 ^# Within test group differences were not significant (P)>0.05 And with 4) and ^# the test group ratio is obvious in difference (P)<0.05). Therefore, different feeding methods have certain protection rate on tilapia, 4 of the protection rate ^# The immune protection rate of the test group is the highest and can reach 48 percent. Therefore, the method for feeding the expanded compound feed, the clostridium butyricum and the five-yellow powder in a sectional manner in the micropterus salmoides cultivation is a practical method with obvious effect, and overcomes the problem of frequent diseases of cultivation objects.

Example 5

Based on examples 1-2, the effect of different application methods and application amounts of the oxone complex salt tablet and the probiotic agent in the round culture pond after 30 days of the seedlings entering the pond was studied.

Randomly selecting 4 circular culture ponds with the serial number of 1 after the seedlings are put and cultured for 30d in 16 days 8 months in 2020 ^# 、2 ^# 、3 ^# And 4 ^# In which 1 is ^# 、2 ^# As a control group, 3 ^# 、4 ^# For the test group, the stocking was as shown in Table 4.

TABLE 4 stocking of different circular ponds

A single-factor test method is adopted, 4 circular culture ponds are randomly divided into a test group and a control group, and the same stocking mode and technical management measures are adopted. The specific test design is shown in table 5.

Table 5 different test designs

And (3) water quality determination: sampling before putting as the initial index determination of water quality, determining once every 3d days after application, and determining 3 times in total, wherein the sampling time is 10 times per day. The results of the determination of Total Inorganic Nitrogen (TIN) in water are shown in Table 6 ₄ -P assay results table 7.

TABLE 6 Total Inorganic Nitrogen (TIN) measurement results (unit: mg/dm) ³ )

TABLE 7PO ₄ Results of P measurement (unit: mg/dm) ³ )

The results in tables 6 and 7 show that the Total Inorganic Nitrogen (TIN) content of the aquaculture pond water changes with PO after the continuous application of the microbial inoculum and the oxone complex salt tablet ₄ P is the same, total Inorganic Nitrogen (TIN) and PO for each treatment group ₄ The content of P shows different reductions, wherein the average removal rate of Total Inorganic Nitrogen (TIN) of a control group is 19.54 percent, the average removal rate of a test group is 67.09 percent, the test group is improved by 47.55 percent compared with the control group, and the difference between the two is obvious (P)<0.05 ); control group PO ₄ The average removal rate of P is 31.06%, the average removal rate of the test group is 56.65%, the test group is 25.59% higher than that of the control group, and the difference between the P and the P is obvious (P)<0.05). Therefore, the comprehensive water purification effect of applying the potassium hydrogen persulfate composite salt tablets in the culture pond after 12h interval is most obvious, and the ammonia nitrogen, the nitrite nitrogen, the nitrate nitrogen and the PO can be ensured ₄ the-P is in a normal level for a long time, is a composite water treatment technology of green bacteriostasis, advanced oxidation detoxification and probiotics, and solves the problem of poor treatment effect of land-based recirculating aquaculture sewage and tail water.

Application example 1

In the embodiment, 16 days at 4 months and 19 days at 11 months and 19 days at 2021, the land-based circular pond recirculating aquaculture system for the bass in California and the innovative demonstration of the matched aquaculture method thereof are developed in the Liangfengjiang aquaculture base of Guangxi Xuehnhong science and technology Limited company.

(I) this application example 1 California perch land-based circular pond recirculating aquaculture system

There are only two differences from the land-based circular pond recirculating aquaculture system described in example 1, namely 3 circular aquaculture ponds (number 1) ^# 、2 ^# 、3 ^# ) (ii) a The initial stocking conditions of the micropterus salmoides are different, and the initial stocking condition of the micropterus salmoides in the application example 1 is shown in table 8.

TABLE 8 initial stocking of offspring seeds

(II) Lateolabrax salmoides land-based circular pond recirculating aquaculture method of application example 1

In contrast to the probiotic preparation used alone in the cultivation method described in example 2, an aqueous preparation of Rhodopseudomonas palustris (the effective viable count is preferably not less than 8.0X 10) is used before water testing and during water adjustment in the early stage of cultivation ⁸ CFU/mL); during the water regulation in the middle and later period of cultivation, bacillus subtilis preparation (the effective viable count is preferably more than or equal to 100 × 10) ⁸ CFU/g)。

The results of the catch after 7 months of cultivation are shown in Table 10.

Comparative example 1

In the comparative example, 16 days at 4 months and 19 days at 11 months in 2021, the culture bases of Liangfengjiang, a Guangxi Xuehong science and technology Limited company develop innovation demonstration of a land-based circular pond recirculating aquaculture system and a matched aquaculture method for the micropterus salmoides.

Basic site original land-based circulating water aquaculture system (see figure 3)

Circular breed pond, brush processing pond, microbial treatment pond, aquatic plant processing pond, water purification disinfection pond, tail water biochemical treatment pond, each functional area proportion is 5. The bottom of the culture pond is 1m higher than the top of the culture pond in other functional areas.

3 circular cement culture ponds (number 4) ^# 、5 ^# 、6 ^# ) The pond wall is brick-concrete structure, and thickness more than 26cm, diameter 10m, the pond is dark 1.8m, and the bottom of the pool is concrete clean face, keeps smooth and to the slope of pond center, is hourglass hopper-shaped, and the pond body is long-pending: 140m ³ Actual water volume 120m ³ 。

The brush treatment tank, the microbial treatment tank, the aquatic plant treatment tank, the water purification and disinfection tank and the tail water biochemical treatment tank are all of brick-concrete cement plastering structures, the depth of each of the five tanks is 1.5m, and the volume of each of the five tanks is 80m ³ 。

The system use flow comprises the following steps:

A. the water body of the culture pond 1 overflows and discharges sewage through a bottom drain pipe through a sewage lifting device 2, water is lifted to a sewage separator, large granular substances in the water body are separated and precipitated, and the large granular substances are discharged to a tail water biochemical treatment pond 9 during sewage discharging and cleaning to be finally purified.

B. The separated clean water overflows to a brush treatment tank 4 through a pipeline, after harmful substances in the water body are adsorbed and separated by a brush, the water for cleaning impurities is discharged to a tail water biochemical treatment tank 9 for treatment, and the purified water is discharged to biological treatment tanks (a microbial treatment tank 5 and an aquatic plant treatment tank 6 which are alternately arranged, wherein the nitrate absorbed by plants is generated by carrying out nitrification and denitrification on protein and ammonia nitrogen nitrite contained in the water body through the arranged microbial treatment tank 5 and then is absorbed and filtered by the plants in the aquatic plant treatment tank 6;

C. the water flows to the purification and disinfection tank after being treated by the biological treatment tank, the disinfected and clean water flows back to the culture tank 1 by the backflow lifting device 8, the fish is continuously cultured, the circular filtration is formed repeatedly, the quality of the water in the culture tank 1 is ensured, and the tail water in the tail water biochemical treatment tank 9 is discharged after reaching the standard.

(II) demonstration of the conventional land-based circular pond recirculating aquaculture method for micropterus salmoides in base II

The specific method comprises the following steps:

1) Treatment of culture ponds

And 7d before the fingerlings are put in, the quick lime is used for comprehensively and thoroughly disinfecting the culture pond to obtain a clean culture pond.

2) Treatment of aquaculture water

And (3) injecting water for cultivation into the cultivation system, and filtering the harmful organisms by using a fine silk screen when injecting water, wherein the water injection depth of the cultivation pond is 1.5m.

3) Stocking seedling

The micropterus salmoides fry are legal in source and qualified in quarantine, the micropterus salmoides fry are required to be normal in body shape, disease-free and injury-free, scales and fin rays are complete, and the initial stocking condition is shown in table 9.

TABLE 9 initial stocking conditions

4) Puffed compound feed for feeding micropterus salmoides

The feeding frequency of the expanded compound feed is 2 times/d, and the feeding amount of each time is 2.5 percent of the mass of the fish body.

5) Disease prevention

Regularly supplementing liver and gallbladder protecting products, immunopotentiators and the like in the feed.

6) Water quality control

The water depth between 3 months and 5 months is controlled to be between 1.3m and 1.5m, the water depth between 6 months and 8 months is gradually increased to be between 1.5m and 1.8m, and the water depth between 9 months and 11 months is stabilized to be about 1.8m. And water is not changed during the culture period, new water is supplemented in time, and the water in the pond is kept clean. The dissolved oxygen was controlled to 6mg/L or more. The opening time of the oxygen-increasing water pushing machine in high-temperature seasons is kept above 10h every day, and the microporous oxygen-increasing machine is opened in rainy and muggy weather.

7) Blowdown and circulation

Excrement and residual bait in the pool are discharged into the system every day by means of the self-cleaning function of a circulating water system.

8) Harvesting: the results of the harvest after 7 months of cultivation are shown in Table 10.

TABLE 10 Productivity of each culture pond of application example 1 and comparative example 1

As can be seen from the table 10, compared with the existing culture system and culture method, the survival rate of the micropterus salmoides cultured in 7 months can be improved by 17.5 percent, the average final weight is improved by 34 g/tail, and the reduced yield is improved by 10kg/m ³ (Total yield is determined by the volume of water, for example, 90m ³ Comparative example Water volume 120m ³ No comparability, so the yield is reduced), the selling price is improved by 1 yuan/kg, the breeding cost is reduced by 3.6 yuan/kg, the profit rate is improved by 15.93%, and the yield value is improved by 194 yuan/m ³ The recycling rate of the tail water of the cultivation reaches 90 percent; the problems of overhigh construction cost and operation cost, poor treatment effect on the culture sewage and tail water, low culture density, single culture variety, frequent diseases of culture objects and the like of the conventional system are solved, the edible safety of culture products is ensured, and remarkable economic, social and ecological benefits are obtained. The low-carbon, high-efficiency and zero-pollution discharge of the aquaculture tail water, resource utilization, universality and universality similar to those of pond culture are realized, and the method has great market popularization value.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. A land-based circular pond circulating water culture system is characterized by comprising a circular culture pond, a fish manure sedimentation pond, a tail water collecting pond, a primary sedimentation pond, a secondary sedimentation pond and a tertiary ecological pond;

the bottom of the circular culture pond is provided with a double-drain chassis device, the double-drain chassis device is provided with a water outlet and a sewage outlet, and the water outlet is communicated with the tail water collecting pond through a drainage pipeline; the sewage outlet is communicated with the fish manure sedimentation tank through a sewage pipeline; the fish manure sedimentation tank is provided with a tail water outlet and a manure outlet; the tail water outlet is communicated with the tail water collecting tank through a pipeline;

2. The land-based circular pond recirculating aquaculture system of claim 1, wherein said circular pond is funnel-shaped with bottom inclined downward toward the center by an angle of 5 ° -25 °;

the top end of the side surface of the circular culture pond is provided with a water inlet, the side surface of the circular culture pond is provided with a water pusher, and the central position of the bottom of the circular culture pond is provided with the double-sewage chassis device;

and an oxygenation device and an intelligent monitoring device are also arranged in the circular culture pond.

3. The land-based circular pond recirculating aquaculture system of claim 1, wherein the upper part of said fish-dung sedimentation tank is hollow cylindrical, the lower part is hollow conical, the height of the top of said fish-dung sedimentation tank is lower than the bottom of said circular aquaculture pond;

the top of the tail water collecting pool is lower than the bottom of the circular culture pool.

4. The land-based circular pond recirculating aquaculture system of claim 1, wherein the heights of the bottoms of the primary sedimentation pond, the secondary sedimentation pond and the tertiary ecological pond are sequentially reduced;

and circulating equipment is arranged on a pipeline between the three-stage ecological pond and the circular culture pond.

5. A method of fish farming using the land-based round pond recirculating aquaculture system of any one of claims 1-4, comprising the steps of:

(1) Injecting the filtered clean aquaculture water into a circular aquaculture pond, and then applying a probiotic agent to the aquaculture water;

6. The culture method according to claim 5, wherein the probiotic bacteria agent is one or two of Rhodopseudomonas palustris, bacillus subtilis and Lactobacillus plantarum.

7. The farming method of claim 5 wherein the staging feeding comprises daily feeding, clostridium butyricum feeding, and Wuhuang powder feeding; the daily feeding is feeding of an expanded compound feed, the feeding frequency is 1-4 times/d, and the feeding amount of each time is 1-5% of the mass of the fish body; the frequency of feeding the clostridium butyricum is continuously fed for 2-4 days every 5-10 days, and the feeding amount of the clostridium butyricum accounts for 2-8 per thousand in mass percent of the feeding amount of the puffed compound feed each time; the Wuhuang powder is fed at a high-temperature culture period or a disease development period, the frequency of feeding the Wuhuang powder is continuously fed for 2-4d every 3d-10d, and the feeding amount of the Wuhuang powder accounts for 2-8 per mill of the feeding amount of the puffed compound feed every time in percentage by mass.

8. The cultivation method according to claim 5, wherein the water transfer comprises water transfer at the early stage of cultivation and water transfer at the middle and later stages of cultivation; the water regulation in the early stage of cultivation is to apply the probiotic bacteria agent for 1 time every day after seedlings are put into a pond, wherein the application amount is 0.2-35 mg/L every time, and the probiotic bacteria agent is continuously sprinkled for 3-5 days;

and after the seedlings are planted in the pond for 20-40 days, the water regulation in the middle and later stages of the cultivation is to apply hydrogen potassium persulfate compound salt tablets in the pond, and apply the probiotic bacteria agent after 8-15 hours, wherein the application frequency of the hydrogen potassium persulfate compound salt tablets is once per 2-3 days, the application amount per time is 0.5-2.0mg/L, and the application amount per time of the probiotic bacteria agent is 0.3-80 mg/L.

9. The cultivation method according to claim 5, wherein 1 defecation is carried out after 0.1-2.5 hours of feeding each time, and the defecation time is 20-70 s each time.

10. The culture method according to claim 5, wherein the culture water body is kept circulating for 24 h/day, and the aerator is operated for 24 h/day.