CN107381832A - The purposes of biological water purification agent - Google Patents
The purposes of biological water purification agent Download PDFInfo
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- CN107381832A CN107381832A CN201710640794.1A CN201710640794A CN107381832A CN 107381832 A CN107381832 A CN 107381832A CN 201710640794 A CN201710640794 A CN 201710640794A CN 107381832 A CN107381832 A CN 107381832A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F2003/001—Biological treatment of water, waste water, or sewage using granular carriers or supports for the microorganisms
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
Abstract
The invention discloses the purposes of biological water purification agent, and biological water purification agent is applied into contaminated pool with the amount of application of 40 50g/ mus, and uniformly the full pool is splashed, and applies once within every 7 10 days, the composition and its parts by weight of this biological water purification agent are：30 34 parts of load of microorganisms water purification stone flour, 15 20 parts of nano-sized iron oxide, 23 parts of active peptides, 57 parts of plant extraction liquid, 35 parts of biology enzyme, 40 50 parts of filler, 50 60 parts of distilled water.Have the beneficial effect that：Biological water purification agent can effectively degrade the pollutants such as nitrogen in water body, phosphorus, organic matter, cellulose, heavy metal ion, moreover it is possible to effectively suppress algal grown, reduce the eutrophication of water body, there is stronger flocculation sediment effect to the suspension in water body；Cost of manufacture is cheap, and nontoxic secondary material residual, the cleanser mechanism of action has broad spectrum activity, can be widely applied to pond remove contamination, plant chimney stalk purification, purifying domestic sewage etc..
Description
Technical field
The present invention relates to the application field of water quality cleansing agent, more particularly, to a kind of purposes of biological water purification agent.
Technical background
Domestic pollution source is mainly various detergent and sewage, rubbish, excrement used in urban life etc., mostly nontoxic
Inorganic salts, more containing nitrogen phosphate and sulfur in sanitary sewage, malignant bacteria is more.It is Chinese annual there are about 1/3 industrial wastewater and 90% with
On sanitary sewage it is unprocessed be just discharged into waters, the whole nation has in 1200 a plurality of rivers of monitoring, 850 it is a plurality of be contaminated, 90%
Urban waters above are also polluted, and cause many section fishes and shrimps to disappear, and meet country-level and two level water standard river
Stream only accounts for 32.2%.Pollution is just also being contaminated from shallow-layer to Deep Development, underground water and coastal waters domain seawater, and we can
The water drunk and used unawares is reduced.
Microorganism is ubiquitous in nature, small to arrive individual cells, greatly to global biosphere, from land to ocean, from
Aquaculture, animal husbandry to agricultural, from food industry, pharmaceuticals industry to printing and dyeing, weaving, process hides etc., every to be related to organic matter various
The technological process of industry is all closely bound up with microorganism.It has the characteristics of individual is small, metabolism is vigorous, distribution is wide, quantity is more,
Also there is powerful capacity of decomposition, their activity ensures the material circulation of the ecosystem, purifies the ecosystem, coordinate
The ecological balance, the probiotics based on microorganism research and development is nontoxic, have no side effect, noresidue and secondary pollution, does not produce anti-medicine
Property, it can effectively improve breeding ecological environment, maintain the ecological balance, enhancing cultivates the immunity of object, reduces the hair of disease
It is raw.Cultivate efficient dominant microflora and water quality cleansing agent be made to improve fish-culture water quality, purification natural water and industrial wastewater, have into
This is low, small investment, it is quick, effect is good the advantages of, be the effective way for improving various water pollutions at present, there is wide open
Send out application prospect.
Technology on biological water purification agent has many methods, and prior art is as authorized public number
The B of CN 101333499 Chinese invention patent, discloses a kind of compound biological water purifiers with live bacteria and preparation method thereof,
The inventive method can effectively remove ammonia nitrogen and nitrous in water using the mixture of several bacillus as complex biological water purification agent
Hydrochlorate, improve water pollution, have and make the advantages of simple, but the inventive method is only used as water purification using a kind of microorganism
Agent, there is the shortcomings that catharsis is single, lack flocculate can not play a part of the difficult macromolecular decomposed of sedimentation, it is impossible to drop
The content of heavy metal ion in low stain water body, it is therefore desirable to coordinate other water purification agents to act synergistically, promotional value is limited.
The content of the invention
The purpose of this method is to provide a kind of safe and efficient, the simple biological water purification agent of application process purposes.
Microorganism and its bought enterprise used in the inventive method are：Rich biological factory is opened up in nitrobacteria, Dongxing；Breast
Acid streptococci, Shaanxi Sen Fu natural products Co., Ltd；Bacillus subtilis, Hubei Nong Kegu Eco Science Technologies Co., Ltd；Ground
Clothing bacillus, Cangzhou City Yi Hong animal health-care products Co., Ltd；Rhodopseudomonas palustris, South Pole Sai Erte biotechnologys are limited
Company.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：The purposes of biological water purification agent,
Including：Activate, load, prepare nano-sized iron oxide, apply, specifically including following steps：
Activation：Gangue is put into pulverizer and crushed, 35-65 mesh sieves are crossed, by colliery powder through 780-800 DEG C of calcination activation 2-
3 hours, obtain thermal activated coal gangue support samples；The strong endothermic reaction and dehydration occur in high-temperature calcination process for gangue
Reaction, and discharges certain activity, volumetric expansion after gangue is fired, its surface and is internally formed substantial amounts of micropore, has
There are stronger ion-exchange capacity and larger adsorption capacity, and its is renewable, inexpensive, can be used as microorganism species immobilization
Good carrier；
Load：The accurate active coal gangue carrier for weighing 20-25 parts, is put into iodine flask, the moist heat sterilization at 120-121 DEG C
20-25 minutes, the composite bacteria liquid of 3-5 parts is added, is put into shaking table, it is small with 40-60r/min rotating speeds shake 2-2.5 at 30-33 DEG C
When, unnecessary bacterium solution is then poured out, the glutaraldehyde water solution that 40-50 parts concentration is 2-2.5% is added, 24-36 is crosslinked at 2-4 DEG C
Unnecessary glutaraldehyde is poured out after hour, with sterile water washing 4-6 times, so far composite flora immobilization is completed to produce load of microorganisms net
Water stone flour；Activated carrier mainly handles waste water by physical absorption and chemisorbed, physical absorption be mainly reflected in gangue with
Intermolecular attraction effect between adsorbate, this effect are determined that this absorption does not have by the porous and surface area of gangue
Selectivity, thus have certain Adsorption ability to various pollutants；And chemisorbed is primarily due to deposit in gangue
In substantial amounts of Al, Si isoreactivity materials, it can be combined with adsorbate by chemical chain, its selectivity is strong, it is generally the case that physics
Absorption and chemisorbed exist simultaneously；On the other hand it is various microorganisms because activated carrier has flourishing specific surface area
Life and breeding create good environment, are advantageous to the immobilization of composite flora, composite flora passes through proliferation function and collaboration
Effect is metabolized out polyphenoils, generates the stable ecosystem, the protozoan that makes to have Function For Purifying Water in water, micro- life
Thing and aquatile have recovered activity；
Prepare nano-sized iron oxide：100-120 parts 0.2-0.25mol/L iron nitrate solution is taken, it is fully molten to add 30-45 part urea
Solution, solution is poured into closed glass container, is placed in water-bath or oil bath and is heated to 120-125 DEG C, reacts 2-2.5 hours,
Cooling and aging 3-4 hours are taken out, centrifuges, sediment is washed to neutrality, are then placed in drying box dry in 103-106 DEG C
Dry 12-18 hours, the presoma of rufous is obtained, the presoma is placed in Muffle furnace, 1-1.5 is calcined at 300-330 DEG C
Hour produces nano-sized iron oxide russet；Volume fraction shared by nano oxidized iron surface is big, and surface atom coordination is not complete, with
And the factor such as undersaturated dangling bonds in surface atom can improve surface-active site, make it have very strong energy of adsorption
Power, compared to traditional flocculant, it can the effective impurity such as the suspension in absorption effluent, metal, metal oxide；
Compounding：Weigh load of microorganisms water purification stone flour 30-34 parts, nano-sized iron oxide 15-20 parts, active peptides 2-3 parts, plant carry
Take liquid 5-7 parts, biology enzyme 3-5 parts, filler 40-50 parts, distilled water 50-60 parts uniform stirring mixing 10- at a temperature of 30-32 DEG C
It is granulated after 15 minutes, particle diameter 2-5mm, packs and seal up for safekeeping after drying；The amino acid sequence of active peptides is：
SCASRCKSRCRARRCRARRCGGFYFGKRCICKCFRC；Active peptides can occur with the heavy metal ion in polluted-water
Complex reaction, sedimentation is adsorbed by adsorbent so as to generate complex compound macromolecular, reduces the content of Heavy Metals in Waters ion；Should
Biologic water cleaner can effectively remove ammonia nitrogen and nitrite in water, improve water pollution, organic macromolecule of degrading, and mitigate rich in water
Nutrient laden degree, the organic macromolecule material in water body is resolved into the absorbent carbohydrate of animals and plants, amino acid, vitamin, life
Active substances etc., moreover it is possible to adsorbed to hard-degraded substance, heavy metal ion content of degrading, suppress algal grown, to water body
In suspension have stronger flocculation sediment effect, be a kind of safe and efficient biological water purification agent；
Using：The biological water purification agent particle that compounding is completed and packed is weighed, is applied to the amount of application of 40-50g/ mus dirty
The pool of dye, uniformly the pool is splashed entirely, is applied once per 7-10 days；It is disintegratable that biological water purification agent, which meets water, cleanser
Each component synergy, effectively decompose and go water removal in ammonia nitrogen and nitrite, by the organic macromolecule material in water body
The materials such as carbohydrate, amino acid, vitamin are resolved into, mitigate Eutrophic Extent in water, moreover it is possible to hard-degraded substance is adsorbed,
Degraded heavy metal ion content, suppresses algal grown, has stronger flocculation sediment effect to the suspension in water body, it acts on machine
Reason has broad spectrum activity, thus can be widely applied to pond remove contamination, plant chimney stalk purification, purifying domestic sewage etc., be a kind of peace
Overall height effect, the simple biological water purification agent of application process.
Preferably, composite bacteria liquid be nitrobacteria or streptococcus lactis or bacillus subtilis or bacillus licheniformis or
Rhodopseudomonas palustris；Composite bacteria liquid can effectively remove ammonia nitrogen and nitrite in water, improve water pollution, degrade organic big point
Son, mitigate Eutrophic Extent in water, and form microorganism overall situation with the source micropopulation in water body and purify water jointly.
Preferably, plant extraction liquid is bamboo juice or rose extract solution or olive leaf extract or said mushroom extract；
Active ingredient such as Polyphenols, flavonoids, tannin class in plant extraction liquid can effectively adsorb the stink substance in polluted-water.
Preferably, biology enzyme be amylase or protease or lipase or phosphatase or glycosidase or urase or esterase or
Cellulase；Biology enzyme can open chemical chain more complicated in water pollutant molecule, and enzyme molecule can make reactant molecule
Middle chemical bond is elongated, distorts and deformed, and makes them be easier to be hydrolyzed, thus accelerates the decomposition of organic matter, and it is degraded rapidly
For small molecule.
Preferably, filler is kaolin or bentonite or silica flour or alunite；The gap structure of filler helps to carry
The adsorption capacity of high cleanser.
Compared with prior art, the advantage of the invention is that：1）The manufacturing process of biological water purification agent is simple and easy, right
Application apparatus it is less demanding, both can be applied to lab scale, can also be applied to industrial-scale production；2）Can effectively it degrade in water body
Nitrogen, phosphorus, organic matter, cellulose, the pollutant such as heavy metal ion, moreover it is possible to effectively suppress algal grown, reduce the rich battalion of water body
Fosterization, there is stronger flocculation sediment effect to the suspension in water body；3）Cost of manufacture is cheap, nontoxic secondary material residual, water quality
Cleanser finished product is a kind of safe and nontoxic, efficient biological water purification agent；4）The cleanser mechanism of action has broad spectrum activity, can
Removed contamination with being widely used in pond, plant chimney stalk purification, purifying domestic sewage etc..
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
The purposes of biological water purification agent, comprises the following steps：
1）Gangue is put into pulverizer and crushed, crosses 35 mesh sieves, by colliery powder through 780 DEG C of calcination activations 2 hours, obtains heat
Active coal gangue support samples；Gangue can be as the good carrier of microorganism species immobilization；The accurate activated coal for weighing 20g
Spoil carrier, is put into iodine flask, moist heat sterilization 20 minutes at 120 DEG C, adds 1g nitrobacterias, 1g streptococcus lactis, 1g
Bacillus subtilis, shaking table is put into, is shaken 2 hours with 40r/min rotating speeds at 30 DEG C, then pour out unnecessary bacterium solution, it is dense to add 40g
The glutaraldehyde water solution for 2% is spent, unnecessary glutaraldehyde is poured out after being crosslinked 24 hours at 2 DEG C, it is so far multiple with sterile water washing 4 times
Flora immobilization is closed to complete to produce load of microorganisms water purification stone flour；Composite flora is metabolized out anti-by proliferation function and synergy
Oxidation material, the stable ecosystem is generated, makes the protozoan, microorganism and aquatile for having Function For Purifying Water in water
Activity is recovered；2）100ml 0.2mol/L iron nitrate solution is taken, 30g urea is added and fully dissolves, solution is poured into closed
Glass container in, be placed in water-bath or oil bath and be heated to 120 DEG C, react 2 hours, take out cooling and aging 3 hours, centrifugation point
From, sediment is washed to neutrality, is then placed in drying box and is dried 12 hours in 103 DEG C, obtain the presoma of rufous, will
The presoma is placed in Muffle furnace, is calcined 1 hour at 300 DEG C and is produced nano-sized iron oxide russet；3）Microorganism is weighed to bear
Carry water purification stone flour 30g, nano-sized iron oxide 15g, active peptides 2g, rose extract solution 5g, amylase 1g, protease 2g, kaolinite
Native 40g, distilled water 50g are granulated after uniform stirring mixes 10 minutes at a temperature of 30 DEG C, particle diameter 2mm, are packed after drying
Seal up for safekeeping；The amino acid sequence of active peptides is：SCASRCKSRCRARRCRARRCGGFYFGKRCICKCFRC；Active peptides can
Complex reaction occurs with the heavy metal ion in polluted-water, sedimentation is adsorbed by adsorbent so as to generate complex compound macromolecular,
Reduce the content of Heavy Metals in Waters ion；4）The biological water purification agent particle that compounding is completed and packed is weighed, with 40g/ mus
Amount of application be applied to contaminated pool, uniformly the full pool is splashed, and is applied once within every 7 days；Biological water purification agent meets water
That is disintegratable, each component synergy of cleanser, effectively decomposes and removes ammonia nitrogen and nitrite in water removal, by water body
Organic macromolecule material resolves into the materials such as carbohydrate, amino acid, vitamin, mitigates Eutrophic Extent in water, moreover it is possible to which hardly possible is dropped
Solution material is adsorbed, and heavy metal ion content of degrading, suppresses algal grown, has stronger flocculation to sink the suspension in water body
Shallow lake acts on.
Embodiment 2：
The purposes of biological water purification agent, comprises the following steps：
1）Gangue is put into pulverizer and crushed, crosses 65 mesh sieves, by colliery powder through 800 DEG C of calcination activations 3 hours, obtains heat
Active coal gangue support samples；Volumetric expansion after gangue is fired, its surface and is internally formed substantial amounts of micropore, has stronger
Ion-exchange capacity and larger adsorption capacity, and its is renewable, inexpensive, can be as the good of microorganism species immobilization
Carrier；
2）The accurate active coal gangue carrier for weighing 25g, is put into iodine flask, moist heat sterilization 25 minutes at 121 DEG C, adds 2g
Streptococcus lactis, 3g bacillus licheniformis, are put into shaking table, are shaken 2.5 hours, then poured out more with 60r/min rotating speeds at 33 DEG C
Remaining bacterium solution, the glutaraldehyde water solution that 50g concentration is 2.5% is added, unnecessary glutaraldehyde is poured out after being crosslinked 36 hours at 4 DEG C, with nothing
Bacterium water washing 6 times, so far composite flora immobilization are completed to produce load of microorganisms water purification stone flour；
3）120ml 0.25mol/L iron nitrate solution is taken, 45g urea is added and fully dissolves, solution is poured into closed glass
In container, it is placed in water-bath or oil bath and is heated to 125 DEG C, react 2.5 hours, take out cooling and aging 4 hours, centrifuges, will
Sediment is washed to neutrality, is then placed in drying box and is dried 18 hours in 106 DEG C, the presoma of rufous is obtained, before this
Drive body to be placed in Muffle furnace, be calcined 1.5 hours at 330 DEG C and produce nano-sized iron oxide russet；Nano-sized iron oxide can have
The impurity such as suspension, metal, metal oxide in the absorption effluent of effect；
4）Weigh load of microorganisms water purification stone flour 34g, nano-sized iron oxide 20g, active peptides 3g, olive leaf extract 7g, phosphoric acid
Enzyme 2g, glycosidase 3g, silica flour 50g, distilled water 60g are granulated after uniform stirring mixes 15 minutes at a temperature of 32 DEG C, grain
Footpath is 5mm, packs and seals up for safekeeping after drying；The amino acid sequence of active peptides is：
SCASRCKSRCRARRCRARRCGGFYFGKRCICKCFRC；
5）The biological water purification agent particle that compounding is completed and packed is weighed, contaminated water is applied to the amount of application of 50g/ mus
The pool, uniformly the pool is splashed entirely, is applied once within every 10 days；The biological water purification agent mechanism of action has a broad spectrum activity, therefore can be with
Be widely used in pond remove contamination, plant chimney stalk purification, purifying domestic sewage etc., be it is a kind of it is safe and efficient, application process is simple
Biological water purification agent.
Embodiment 3：
The purposes of biological water purification agent, including：Activate, load, prepare nano-sized iron oxide, apply, specifically including following steps：
Activation：Gangue is put into pulverizer and crushed, crosses 60 mesh sieves, by colliery powder through 800 DEG C of calcination activations 2.5 hours,
Obtain thermal activated coal gangue support samples；The strong endothermic reaction and dehydration occur in high-temperature calcination process for gangue,
And certain activity is discharged, volumetric expansion after gangue is fired, its surface and substantial amounts of micropore is internally formed, had stronger
Ion-exchange capacity and larger adsorption capacity, and its is renewable, inexpensive, can be as the good of microorganism species immobilization
Carrier；
Load：The accurate active coal gangue carrier for weighing 25 parts, is put into iodine flask, moist heat sterilization 20 minutes, add at 121 DEG C
Enter 4 parts of composite bacteria liquid, be put into shaking table, shaken 2.5 hours with 50r/min rotating speeds at 32 DEG C, then pour out unnecessary bacterium solution, added
The glutaraldehyde water solution that 50 parts of concentration are 2.5%, pours out unnecessary glutaraldehyde, with sterile water washing 6 after being crosslinked 24 hours at 4 DEG C
Secondary, so far composite flora immobilization is completed to produce load of microorganisms water purification stone flour；Activated carrier has flourishing specific surface area, is
The life and breeding of various microorganisms create good environment, are advantageous to the immobilization of composite flora, and composite flora passes through increasing
The effect of growing and synergy are metabolized out polyphenoils, generate the stable ecosystem, make have Function For Purifying Water in water
Protozoan, microorganism and aquatile have recovered activity；
Prepare nano-sized iron oxide：120 parts of 0.25mol/L iron nitrate solution is taken, 40 parts of urea is added and fully dissolves, solution is fallen
Enter in closed glass container, be placed in water-bath or oil bath and be heated to 120 DEG C, react 2 hours, take out cooling and aging 3 hours, from
The heart is separated, and sediment is washed into neutrality, is then placed in drying box and is dried 12 hours in 106 DEG C, obtains the forerunner of rufous
Body, the presoma is placed in Muffle furnace, is calcined 1 hour at 300 DEG C and produces nano-sized iron oxide russet；Nano-sized iron oxide
Volume fraction shared by surface is big, and surface atom coordination is not complete, and the factor such as undersaturated dangling bonds in surface atom can
To improve surface-active site, very strong adsorption capacity is made it have, compared to traditional flocculant, it can effectively adsorb dirt
The impurity such as suspension, metal, metal oxide in water；
Compounding：Weigh 32 parts of load of microorganisms water purification stone flour, 18 parts of nano-sized iron oxide, 2 parts of active peptides, 5 parts of plant extraction liquid,
4 parts of biology enzyme, 45 parts of filler, 50 parts of distilled water are granulated at a temperature of 30 DEG C after uniform stirring mixes 10 minutes, and particle diameter is
4mm, pack and seal up for safekeeping after drying；The amino acid sequence of active peptides is：
SCASRCKSRCRARRCRARRCGGFYFGKRCICKCFRC；Active peptides can occur with the heavy metal ion in polluted-water
Complex reaction, sedimentation is adsorbed by adsorbent so as to generate complex compound macromolecular, reduces the content of Heavy Metals in Waters ion；Should
Biologic water cleaner can effectively remove ammonia nitrogen and nitrite in water, improve water pollution, organic macromolecule of degrading, and mitigate rich in water
Nutrient laden degree, the organic macromolecule material in water body is resolved into the absorbent carbohydrate of animals and plants, amino acid, vitamin, life
Active substances etc., moreover it is possible to adsorbed to hard-degraded substance, heavy metal ion content of degrading, suppress algal grown, to water body
In suspension have stronger flocculation sediment effect, be a kind of safe and efficient biological water purification agent；
Using：The biological water purification agent particle that compounding is completed and packed is weighed, is applied to the amount of application of 45g/ mus contaminated
Pool, uniformly the full pool splash, apply once within every 8 days；It is disintegratable that biological water purification agent, which meets water, cleanser it is each
Component acts synergistically, and effectively decomposes and removes ammonia nitrogen and nitrite in water removal, the organic macromolecule material in water body is resolved into
The materials such as carbohydrate, amino acid, vitamin, mitigate Eutrophic Extent in water, moreover it is possible to hard-degraded substance is adsorbed, degraded weight
Metal ion content, suppress algal grown, there is stronger flocculation sediment effect to the suspension in water body, its mechanism of action has
Broad spectrum activity, thus can be widely applied to pond remove contamination, plant chimney stalk purification, purifying domestic sewage etc., be it is a kind of it is safe and efficient,
The simple biological water purification agent of application process.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Animal feed technological development Co., Ltd is full of by Pujiang County association
<120>The purposes of biological water purification agent
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 36
<212> PRT
<213>It is artificial synthesized
<400> 1
Ser Cys Ala Ser Arg Cys Lys Ser Arg Cys Arg Ala Arg Arg Cys Arg
1 5 10 15
Ala Arg Arg Cys Gly Gly Phe Tyr Phe Gly Lys Arg Cys Ile Cys Lys
20 25 30
Cys Phe Arg Cys
35
Claims (10)
1. the purposes of biological water purification agent, the biological water purification agent particle that compounding is completed and packed is weighed, with 40-50g/ mus
Amount of application be applied to contaminated pool, uniformly the full pool is splashed, and is applied once per 7-10 days, it is characterised in that：Biology
A kind of active peptides are added in the configuration step of water quality cleansing agent.
2. biological water purification agent according to claim 1, it is characterised in that comprise the following steps：
1）Activation：Colliery powder high-temperature roasting is activated；
2）Load：Active coal gangue carrier is loaded with complex microorganism；
3）Prepare nano-sized iron oxide：Preparation of nano-sized iron oxide by precipitation method；
4）Compounding：Complex biological water quality cleansing agent；
5）Using：Biological water purification agent is applied in a manner of full pool spilling head at regular time and quantity.
3. the preparation method of biological water purification agent according to claim 2, it is characterised in that：Described activation step
For：Gangue is put into pulverizer and crushed, crosses 35-65 mesh sieves, by colliery powder through 780-800 DEG C of calcination activation 2-3 hour,
Obtain thermal activated coal gangue support samples.
4. the preparation method of biological water purification agent according to claim 2, it is characterised in that：Described load step
For：The accurate active coal gangue carrier for weighing 20-25 parts, is put into iodine flask, moist heat sterilization 20-25 points at 120-121 DEG C
Clock, the composite bacteria liquid of 3-5 parts is added, is put into shaking table, 2-2.5 hours, Ran Houqing are shaken with 40-60r/min rotating speeds at 30-33 DEG C
Go out unnecessary bacterium solution, add the glutaraldehyde water solution that 40-50 parts concentration is 2-2.5%, poured out after being crosslinked 24-36 hours at 2-4 DEG C
Unnecessary glutaraldehyde, with sterile water washing 4-6 times, so far composite flora immobilization is completed to produce load of microorganisms water purification stone flour.
5. the preparation method of biological water purification agent according to claim 2, it is characterised in that：In described load step
Composite bacteria liquid be：The red false unit cell of nitrobacteria or streptococcus lactis or bacillus subtilis or bacillus licheniformis or marsh
Bacterium.
6. the preparation method of biological water purification agent according to claim 2, it is characterised in that：Described preparation nano oxygen
Changing iron step is：100-120 parts 0.2-0.25mol/L iron nitrate solution is taken, 30-45 part urea is added and fully dissolves, will be molten
Liquid is poured into closed glass container, is placed in water-bath or oil bath and is heated to 120-125 DEG C, reacts 2-2.5 hours, takes out cooling
3-4 hours are aged, centrifuges, sediment is washed to neutrality, is then placed in drying box in 103-106 DEG C of dry 12-18
Hour, obtain the presoma of rufous, the presoma be placed in Muffle furnace, at 300-330 DEG C be calcined 1-1.5 hours produce
Nano-sized iron oxide russet.
7. the preparation method of biological water purification agent according to claim 2, it is characterised in that：Described compounding step
For：Weigh load of microorganisms water purification stone flour 30-34 parts, nano-sized iron oxide 15-20 parts, active peptides 2-3 parts, plant extraction liquid 5-
7 parts, biology enzyme 3-5 parts, filler 40-50 parts, distilled water 50-60 parts uniform stirring mixing 10-15 minutes at a temperature of 30-32 DEG C
After be granulated, particle diameter 2-5mm, pack and seal up for safekeeping after drying.
8. the preparation method of biological water purification agent according to claim 2, it is characterised in that：In described compounding step
The amino acid sequences of active peptides be：SCASRCKSRCRARRCRARRCGGFYFGKRCICKCFRC.
9. the preparation method of biological water purification agent according to claim 2, it is characterised in that：In described compounding step
Plant extraction liquid be bamboo juice or rose extract solution or olive leaf extract or said mushroom extract；Biology enzyme be amylase or
Protease or lipase or phosphatase or glycosidase or urase or esterase or cellulase；Filler is kaolin or bentonite or stone
English powder or alunite.
10. the preparation method of biological water purification agent according to claim 2, it is characterised in that：Described step of applying
For：Using：The biological water purification agent particle that compounding is completed and packed is weighed, is applied to the amount of application of 40-50g/ mus dirty
The pool of dye, uniformly the pool is splashed entirely, is applied once per 7-10 days.
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| CN108423933A (en) * | 2018-03-23 | 2018-08-21 | 威士邦（厦门）环境科技有限公司 | A kind of water purifying device |
| CN108423933B (en) * | 2018-03-23 | 2024-02-23 | 威士邦（厦门）环境科技有限公司 | Water body purifying device |
| CN108975526A (en) * | 2018-07-24 | 2018-12-11 | 芜湖市棠华建材科技有限公司 | Fructus Cucurbitae moschatae extract composite scale sustained release agent |
| CN108975471A (en) * | 2018-07-24 | 2018-12-11 | 芜湖市棠华建材科技有限公司 | Compound oil field wastewater treatment agent |
| CN109874639B (en) * | 2019-03-21 | 2024-02-02 | 金陵科技学院 | Ecological engineering system for retaining water and soil in farmland sand canal |
| CN109874639A (en) * | 2019-03-21 | 2019-06-14 | 金陵科技学院 | A kind of farmland sand irrigation canals and ditches soil and water conservation works system |
| CN110104891A (en) * | 2019-05-24 | 2019-08-09 | 衡水英利新能源有限公司 | A method of drop removes photovoltaic cell heavy metal ions in wastewater |
| CN113277627A (en) * | 2021-04-19 | 2021-08-20 | 广西壮族自治区环境保护科学研究院 | Method for treating and recycling urban black and odorous water sludge |
| CN113277627B (en) * | 2021-04-19 | 2022-10-04 | 广西壮族自治区环境保护科学研究院 | Method for treating and recycling urban black and odorous water sludge |
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