CN105779331A - Biological strain nutritional agent and preparation method and application thereof - Google Patents
Biological strain nutritional agent and preparation method and application thereof Download PDFInfo
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Abstract
The invention provides a biological strain nutritional agent and a preparation method and application thereof.The biological strain nutritional agent comprises, by weight, 30-70 parts of urea, 350-600 pats of alcoholase powder, 200-400 parts of soybean cakes and 50-150 parts of yeast powder, wherein the alcoholase powder comprises, by mass, 60-65% of gibberella zeae enzyme, 18-19% of xylitol enzyme, 10-11% of cellulose, 0.3-0.5% of pectinase, 1-3% of amylase and 5-7% of protease.The biological strain nutritional agent is rich in nutrition and capable of enriching nutrition required by organisms in sewage, and therefore reproduction and metabolism of microorganisms are promoted; activity of the microorganisms in a sewage system is improved, and the number of the microorganisms in the sewage system is increased; the microorganisms in sludge can be effectively acclimated, so that activity of the microorganisms in the system is intensified; it is guaranteed that pollutants are utilized and decomposed to the greatest extent, and the effect of treating sewage is achieved.
Description
Technical field
The present invention relates to microorganism field, in particular to a kind of biological inoculum nutritional agents
And its preparation method and application.
Background technology
Sewage is that be often referred to necessarily to be polluted, from life and the discharge water that produces.Sewage
Mainly there are sanitary sewage, industrial wastewater and early-stage rainwater.The major pollutants of sewage have cause of disease
Body pollution thing, oxygen consumption pollutant, plant nutrient and toxic pollutant etc..Drastically influence
Natural environment.But, sewage disposal system costly, debugging time length, efficiency low.
After sewage enters water body, on the one hand water body is produced and pollute, on the other hand water body itself
There is certain ability purifying sewage, i.e. through physics, chemistry and the biological effect of water body,
The concentration making dirty water pollutant is minimized, and through after a period of time, water body tends to extensive
Arrive contaminated front state again, and decompose under the effect of microorganism, so that water body
Being reverted to cleaning by unclean, this process is referred to as the self purification process of water body.Micro-life in sewage
Thing, as other life tellurian, needs nutrition to enhance metabolism, grows and numerous
Grow.When lacking enough nutrition in Sewage Environment, the metabolism of cell individual can be subject to
Suppression, microbial population activity decrease, quantity reduce, and finally cause sewage disposal system
Operation problem, such as: sludge bulking, offscum increase, settling property is poor, suspended solid can not
Effectively remove, mud is too much, BOD or COD clearance is low.
Owing in the water body system of sewage, microbial biomass is few, kind is single, to water quantity and quality
The reasons such as adaptive faculty is poor in change, nutrition in water is single, ultimately result in sewage self-cleanse weak effect.
In view of this, the special proposition present invention.
Summary of the invention
The first object of the present invention is to provide a kind of biological inoculum nutritional agents, to solve sewage
Middle microbial biomass is few, kind is single, poor to water quantity and quality adaptive faculty, sewage Middle nutrition is single
Problem.Described biological inoculum nutritional agents has the activity of microorganism in raising sewerage
With quantity, reach the advantage such as balanced in nutrition.Can the effective microorganism in acclimation sludge, from
And the activity of microorganism in consolidation system, reach to process the effect of sewage.
The second object of the present invention is to provide the preparation of a kind of described biological inoculum nutritional agents
Method, the method through pulverizing, mix, this biological inoculum nutritional agents is prepared in the operation stirred,
Have conveniently, the advantage such as simple, easily operated.
In order to realize the above-mentioned purpose of the present invention, spy by the following technical solutions:
A kind of biological inoculum nutritional agents, the following component including preparing according to parts by weight:
Urea 30-70 part, alcohol enzyme powder 350-600 part, soya-bean cake 200-400 part and dusty yeast
50-150 part；
Wherein, being calculated in mass percent, described alcohol enzyme powder includes following component:
Gibberella zeae enzyme 60-65%, wood sugar alcoholase 18-19%, cellulase 10-11%, really
Glue enzyme 0.3-0.5%, amylase 1-3% and protease 5-7%.
Biological nutritive agent is formulated with proprietary water soluble polymerizer leachate, Qi Zhongfu
Containing nutriments such as trace element, amino acid, the breeding of aerobic beneficial bacterium can be promoted, carry
The high microorganism oxidative decomposition capacity to pollutant.
Urea, also known as phosphoamide (Carbamide), is a kind of white crystal.For in sewage
Microorganism provide needed for nitrogen source, nitrogen source is nutrition necessary to microbial growth and metabolism
One of material, is mainly used in synthetic protein, nucleic acid and nitrogenous metabolite.Urea
Molecular formula be H2NCONH2, in the nitrogen source waiting quality, its nitrogen element content is high, can
Think that microorganism provides sufficient nutrition.
Enzyme, refers to the polymer substance with biocatalytic Activity.Catalystic converter system at enzyme
In, reactant molecule is referred to as substrate, and substrate is catalytically conveted to another kind of molecule by enzyme.
Almost all of cellular activity process is required for the participation of enzyme, to improve efficiency.Institute of the present invention
Enzyme is provided in the nutritional agents provided, i.e. promotes the microbial metabolism in water and breeding.
Soya-bean cake is the residue that soybean extracts after oil, and use that milling process extracts after oil is big
Beans residue, because great majority are pressed into pie, so being referred to as soya-bean cake.Soybean cake crude protein
Content is high, and possibly together with multiple necessary amino acid, and amino acid composition is rationally, lysine and essence
The ratio of propylhomoserin is more appropriate, about 10:13.Wherein, during lysine is protein uniquely
With the amino acid of side chain primary amino radical, 1B is common 20 kinds of amino of constitutive protein matter
A kind of basic amino acid in acid, is one of microbial reproduction institute essential amino acid.Additionally,
Possibly together with substantial amounts of vitamin, mineral matter and growth factor in soya-bean cake.Therefore, soya-bean cake can promote
Enter the breeding of microorganism, and the ability improving microbe oxidation Decomposition is had
Remarkable effect.
Alcohol enzyme powder is a kind of product of applicant's autonomous production, mainly include ZER,
Cellulase, amylase, protease, wood sugar alcoholase and pectase, and through mixing and stirring
Mix and be prepared from.Can the effectively ANFs in decomposing organism bacterial classification nutritional agents, release battalion
Support material so that it is can fully be digested and assimilated, improve the utilization rate of biological inoculum nutritional agents,
Strengthen and improve breeding production performance and the metabolic capabilities of microbial bacteria.Mainly act on
Biological inoculum nutritional agents raw material, first destroys the intercellular layer of plant cell wall, destroys plant thin
Cell wall structures, makes cellular content exposed, discharges wrapped nutriment, at hydrochloric acid in gastric juice
In more stable and can be with continuous action in biomass, digestive ferment endogenous with animal connects
Touch and be degraded, decreasing microbial bacteria chyme viscosity simultaneously and nutrien utilization and absorption are carried
The negative effect come.
Wherein, cellulose can be hydrolyzed to glucose by cellulase thoroughly, is greatly improved
Biological inoculum production performance and biological inoculum nutritional agents utilization rate；Amylase can be by biological inoculum
In nutritional agents the difficult macromolecules starch absorbed be decomposed into the most absorbed in, lower-molecular substance；
Protease can will be primarily present protease inhibitors and two kinds of anti-battalion of agglutinin in beans nutritional agents
Supporting the factor, the healthy of microbial bacteria can be produced infringement, protease can added body endoproteinase
The deficiency of secretion, improves the digestibility of protein；ZER can directly or indirectly improve
Microbial body inner growth hormone, promotes the synthesis of body protein, improves the life of microbial bacteria
Long acting, thus promote the breeding effect of microbial bacteria；Zytase effectively degrade AX,
Eliminate AX to the anti-oxidant action of microbial bacteria, improve biological bacteria resource utilization；Pectin
Enzyme can the ANFs such as pectin and cellulose in degrading plant cell membrane, make nutriment
Discharge, thus improve the nutritive value of biological inoculum nutritional agents, promote microbial bacteria
Digest and assimilate.
Under the catalytic action of enzyme, nutritional labeling changes into rapidly and to be easily absorbed by the micro-organisms
Material, and the metabolism to microorganism has facilitation, makes microorganism breed rapidly,
Purify sewage.
Preferably, described biological inoculum nutritional agents, specifically include and prepare according to parts by weight
Following component:
Urea 40-60 part, alcohol enzyme powder 400-500 part, soya-bean cake 250-350 part, dusty yeast
80-120 part.
Preferably, described biological inoculum nutritional agents, in terms of parts by weight, also include as follows
Component: glucose 15-25 part.
Glucose is to provide necessary carbon source for microorganism, and carbon source is mainly used for constituting micro-
Biological cellular material and some metabolites, be also the main energy sources material of heterotrophic microorganism.
Can the division of some microorganism, autotrophic microbe and heterotrophic microorganism type is mainly dependence
With CO2As the main of growth or sole carbon source, if the content of heterotrophic microorganism in sewage
Too high, then require supplementation with glucose as carbon source.
Preferably, described biological inoculum nutritional agents, in terms of parts by weight, also include: sulphur
One or more in acid copper 15-25 part, ferrous sulfate 15-25 part and zinc sulfate 15-25 part.
The heavy metal (such as copper, zinc, iron, nickel and cobalt) of trace be bioenzyme activity auxiliary because of
Son, is Institute of Micro-biology's indispensable element, and most organism concentration of heavy metal ion have reached 1mg/L
Level, organism toxicosis can be made.Therefore, be according to the particular make-up of handled sewage
Determine the addition of copper sulphate, ferrous sulfate and zinc sulfate.
Preferably, described biological inoculum nutritional agents, in terms of parts by weight, also include: rely
Propylhomoserin 15-25 part.
The metabolism of microorganism and breeding not only require supplementation with C, H, O, N, in addition it is also necessary to supplement
Growth factor.Lysine is a kind of important growth factor of microbial metabolism, is mainly used in
Participate in protein, enzyme and the generation of polypeptide.Amino needed for soya-bean cake is not enough to offer microorganism
During acid, lysine to be supplemented in time for microbial metabolism and breeding.
Preferably, described yeast is dry ferment；Dry ferment is prone to preserve.
The preparation method of a kind of described biological inoculum nutritional agents, comprises the following steps:
Above-mentioned raw materials is pulverized, obtains crushed material, then by crushed material according to set heavy
Amount number carries out mixing, stirring, and obtains biological inoculum nutritional agents.
Raw material first to be pulverized, and is then mixed by crushed material, stirs, obtains this biological bacteria
Plant nutritional agents.
Preferably, the particle diameter of described crushed material is 15-25 micron.
The particle of 15-25 micron, it is simple to it dissolves in a liquid, disperses and spreads.
Preferably, the time of described stirring is 15-25 minute.
Sufficiently mixing time, makes nutriment be uniformly dispersed, it is simple to during use, nutritional agents
Sewage disperses and spreads.
The application of a kind of described biological inoculum nutritional agents, uses described biological bacteria in sewage
The amount planting nutritional agents is 90-120g/m3。
Addition is at 90-120g/m3Between, it is both in liberal supply to the microbial nutrition in sewage,
Turn avoid overnutrition, be that the biological inoculum nutritional agents that the present invention provides is suitable for addition.
Compared with prior art, the invention have the benefit that
(1) the biological inoculum nutritional agents that the present invention provides, nutritious, can enrich sewage
In biological necessary nutrition, overcome because single run system of pollutant component is caused
Risk, thus promote breeding and the metabolism of microorganism, improve micro-life in sewerage
The activity of thing and quantity.
(2) the biological inoculum nutritional agents that the present invention provides, can effective micro-in acclimation sludge
Biology, thus the activity of microorganism in consolidation system, it is ensured that the utilization of pollutant to greatest extent
And decomposition, reach to process the effect of sewage.
(3) preparation method of the biological inoculum nutritional agents that the present invention provides, the method is through powder
Broken, mix, this biological inoculum nutritional agents is prepared in the operation stirred, have conveniently, simple,
The advantage such as easily operated.
Detailed description of the invention
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but this
Skilled person is it will be appreciated that the following example is merely to illustrate the present invention, and should not regard
For limiting the scope of the present invention.Unreceipted actual conditions person in embodiment, according to normal condition
Or the condition of manufacturer's suggestion is carried out.Agents useful for same or instrument unreceipted production firm person, all
For the conventional products that can be obtained by commercially available purchase.
Embodiment 1
The biological inoculum nutritional agents that the present embodiment provides, including following components:
Urea 30g, alcohol enzyme powder 350g, soya-bean cake 200g, dusty yeast 50g；
Wherein, alcohol enzyme powder includes the following component of mass percentage:
Gibberella zeae enzyme 60%, wood sugar alcoholase 19%, cellulase 11 %, pectase 0.5%,
Amylase 3% and protease 6.5%.
The preparation method of this biological inoculum nutritional agents comprises the following steps:
Above-mentioned raw materials is pulverized, obtains crushed material, then by crushed material according to set heavy
Amount number carries out mixing, stirring, and obtains biological inoculum nutritional agents.
Embodiment 2
The biological inoculum nutritional agents that the present embodiment provides, including following components:
Urea 70g, alcohol enzyme powder 600g, soya-bean cake 400g, dusty yeast 150g；
Wherein, alcohol enzyme powder includes the following component of mass percentage:
Gibberella zeae enzyme 65%, wood sugar alcoholase 18%, cellulase 10%, pectase 0.3%,
Amylase 1% and protease 5.7%.
The preparation method of this biological inoculum nutritional agents comprises the following steps:
Above-mentioned raw materials is pulverized, obtains 15 powder and micron particles, then by crushed material according to
Set parts by weight mix, and stir 15 minutes, obtain biological inoculum nutritional agents.
Embodiment 3
The biological inoculum nutritional agents that the present embodiment provides, including following components:
Urea 50g, alcohol enzyme powder 450g, soya-bean cake 300g, dusty yeast 100g, glucose 20g；
Wherein, alcohol enzyme powder includes the following component of mass percentage:
Gibberella zeae enzyme 62%, wood sugar alcoholase 19%, cellulase 11 %, pectase 0.4%,
Amylase 2 .6% and protease 5%.The preparation method of this biological inoculum nutritional agents includes following
Step:
Above-mentioned raw materials is pulverized, obtains 25 powder and micron particles, then by crushed material according to
Set parts by weight mix, and stir 25 minutes, obtain biological inoculum nutritional agents.
Embodiment 4
The biological inoculum nutritional agents that the present embodiment provides, including following components:
Urea 50g, alcohol enzyme powder 350g, soya-bean cake 300g, dusty yeast 100g, glucose 20g,
Copper sulphate 15g；
Wherein, alcohol enzyme powder includes the following component of mass percentage:
Gibberella zeae enzyme 60%, wood sugar alcoholase 18.5%, cellulase 10.5%, pectase
0.5%, amylase 2 .5% and protease 7%.
The preparation method of this biological inoculum nutritional agents comprises the following steps:
Above-mentioned raw materials is pulverized, obtains 15 powder and micron particles, then by crushed material according to
Set parts by weight mix, and stir 25 minutes, obtain biological inoculum nutritional agents.
Embodiment 5
The biological inoculum nutritional agents that the present embodiment provides, including following components:
Urea 50g, alcohol enzyme powder 450g, soya-bean cake 200g, dusty yeast 50g, glucose 15g,
Copper sulphate 25g, ferrous sulfate 15g, zinc sulfate 15g；
Wherein, alcohol enzyme powder includes the following component of mass percentage:
Gibberella zeae enzyme 63%, wood sugar alcoholase 18%, cellulase 11 %, pectase
0.5%, amylase 1.5% and protease 6%.The preparation side of this biological inoculum nutritional agents
Method comprises the following steps:
Above-mentioned raw materials is pulverized, obtains 20 powder and micron particles, then by crushed material according to
Set parts by weight mix, and stir 15 minutes, obtain biological inoculum nutritional agents.
Embodiment 6
The biological inoculum nutritional agents that the present embodiment provides, including following components:
Urea 30-70g, alcohol enzyme powder 350-600g, soya-bean cake 200-400g, dusty yeast 50-150g,
Glucose 15-25g, copper sulphate 15-25g, ferrous sulfate 15-25g, zinc sulfate 15-25g,
Lysine 15-25g；
Wherein, alcohol enzyme powder includes the following component of mass percentage:
Gibberella zeae enzyme 63.5%, wood sugar alcoholase 18.7%, cellulase 10.6%, pectin
Enzyme 0.3%, amylase 1.8% and protease 5.1%.
The preparation method of this biological inoculum nutritional agents comprises the following steps:
Above-mentioned raw materials is pulverized, obtains 20 powder and micron particles, then by crushed material according to
Set parts by weight mix, and stir 25 minutes, obtain biological inoculum nutritional agents.
Embodiment 7
The biological inoculum nutritional agents that the present embodiment provides, including following components:
Urea 30-70g, alcohol enzyme powder 350-600g, soya-bean cake 200-400g, dusty yeast 50-150g,
Glucose 15-25g, copper sulphate 15-25g, ferrous sulfate 15-25g, zinc sulfate 15-25g,
Lysine 15-25g；
Wherein, alcohol enzyme powder includes the following component of mass percentage:
Gibberella zeae enzyme 63.56%, wood sugar alcoholase 18.64%, cellulase 10.59%, really
Glue enzyme 0.32%, amylase 1.59% and protease 5.30%.
The preparation method of this biological inoculum nutritional agents comprises the following steps:
Above-mentioned raw materials is pulverized, obtains 15 powder and micron particles, then by crushed material according to
Set parts by weight mix, and stir 20 minutes, obtain biological inoculum nutritional agents.
Embodiment 8
The biological inoculum nutritional agents that the present embodiment provides, including following components:
Urea 50g, alcohol enzyme powder 450g, soya-bean cake 300g, dusty yeast 100g, glucose 20g,
Copper sulphate 20g, ferrous sulfate 20g, zinc sulfate 20g, lysine 20g；
Wherein, alcohol enzyme powder includes the following component of mass percentage:
Gibberella zeae enzyme 62.5%, wood sugar alcoholase 18.33%, cellulase 10.42%, really
Glue enzyme 0.42%, amylase 2 .08% and protease 6.25%.
The preparation method of this biological inoculum nutritional agents comprises the following steps:
Above-mentioned raw materials is pulverized, obtains 20 powder and micron particles, then by crushed material according to
Set parts by weight mix, and stir 20 minutes, obtain biological inoculum nutritional agents.
Embodiment 9
The biological inoculum nutritional agents that in the present invention, embodiment 8 is provided is used in cesspool
90g/m3。
Embodiment 10
The biological inoculum nutritional agents that in the present invention, embodiment 8 is provided is used in cesspool
120g/m3。
Experimental example 1 biological inoculum nutritional agents nutrient composition content is tested
Testing the nutritional labeling in embodiment 1-8, this biological inoculum nutritional agents is shallow
Brown particles, the test result of the biological inoculum nutritional agents of per kilogram is as shown in table 1.
Table 1 nutritional labeling test result
It is demonstrated experimentally that the pH value of the solution of this biological inoculum nutritional agents that mass fraction is 1%
For 6-7.7, in weak acid with alkalescent, it is suitable for microbe survival and breeding.Further, this biology
Bacterial classification nutritional agents is also rich in element necessary to the microorganism such as amino acid and C, N, P.Contain
There is abundant carbohydrate, can be that the breeding of microorganism provides sufficient nutrition, nutrition
Equilibrium release, release appropriateness, nutrition supply persistently promote microbial reproduction.Additionally, this battalion
Support agent possibly together with abundant enzyme, promotion bacteria metabolism.Wherein embodiment 8 is optimum
Embodiment.
Experimental example 2 wastewater purifying efficiency is tested
Application process to the biological inoculum nutritional agents that the present invention of embodiment 9 and 10 provides,
Being administered the test of before and after's wastewater treatment efficiency, experimental result is as shown in table 2.
In sewage, major microorganisms has: campanularian, etc. branch worm, fine worm, lid fine worm, bunching
Worm and the anchorage bion such as various metazoa and suction pipe insects are biological.
Test event mainly have BOD (biochemical oxygen demand (BOD)), COD (COD),
SS (suspended solid), pH value, ammonia nitrogen, total phosphorus, colourity；
Table 2 wastewater treatment efficiency is tested
It is demonstrated experimentally that use the biological inoculum nutritional agents that this invention is provided, can be with specific aim
Solve malnourished problem so that microbial activity and reproductive capacity strengthen, to pollutant
Decomposition and the impact resistance of water quality and quantity is improved, finally make system treatment effeciency improve,
Water quality reaching standard.
In sum, the biological inoculum nutritional agents that the present invention provides, nutritious, Ke Yifeng
Biological necessary nutrition in rich sewage, overcomes and transports system because pollutant component is single
The risk that row causes, thus promote breeding and the metabolism of microorganism, improve sewerage
The activity of middle microorganism and quantity.This biological inoculum nutritional agents, can be effectively in acclimation sludge
Microorganism, thus the activity of microorganism in consolidation system, it is ensured that pollutant to greatest extent
Utilize and decompose, reaching to process the effect of sewage.The preparation method of this biological inoculum nutritional agents,
The method prepares this biological inoculum nutritional agents through the operation pulverized, mix, stir, and has
The advantage such as convenient, simple, easily operated.Use the biological inoculum nutrition that this invention is provided
Agent, specific aim solves malnourished problem so that microbial activity and reproductive capacity strengthen,
Decomposition to pollutant and the impact resistance to water quality and quantity improve, and finally system are processed
Efficiency improves, water quality reaching standard.
Although illustrate and describing the present invention with specific embodiment, but it will be appreciated that
May be made that without departing from the spirit and scope of the present invention many other change and
Amendment.It is, therefore, intended that include in the following claims belonging in the scope of the invention
All such changes and modifications.
Claims (10)
1. a biological inoculum nutritional agents, it is characterised in that include preparing according to parts by weight
Following component:
Urea 30-70 part, alcohol enzyme powder 350-600 part, soya-bean cake 200-400 part and dusty yeast
50-150 part；
Wherein, being calculated in mass percent, described alcohol enzyme powder includes following component:
Gibberella zeae enzyme 60-65%, wood sugar alcoholase 18-19%, cellulase 10-11%, really
Glue enzyme 0.3-0.5%, amylase 1-3% and protease 5-7%.
Biological inoculum nutritional agents the most according to claim 1, it is characterised in that concrete
Following component including preparing according to parts by weight:
Urea 40-60 part, alcohol enzyme powder 400-500 part, soya-bean cake 250-350 part, dusty yeast
80-120 part.
Biological inoculum nutritional agents the most according to claim 1 and 2, it is characterised in that
In terms of parts by weight, also include following component: glucose 15-25 part.
Biological inoculum nutritional agents the most according to claim 3, it is characterised in that with weight
Amount number meter, also includes: copper sulphate 15-25 part, ferrous sulfate 15-25 part and zinc sulfate
One or more in 15-25 part.
Biological inoculum nutritional agents the most according to claim 4, it is characterised in that with weight
Amount number meter, also includes: lysine 15-25 part.
Biological inoculum nutritional agents the most according to claim 1 and 2, it is characterised in that
Described yeast is dry ferment.
7. the preparation side according to the biological inoculum nutritional agents described in any one of right 1-6
Method, it is characterised in that comprise the following steps:
Above-mentioned raw materials is pulverized, obtains crushed material, then by crushed material according to set heavy
Amount number carries out mixing, stirring, and obtains biological inoculum nutritional agents.
The preparation method of biological inoculum nutritional agents the most according to claim 7, its feature
Being, the particle diameter of described crushed material is 15-25 micron.
The preparation method of biological inoculum nutritional agents the most according to claim 7, its feature
Being, the time of described stirring is 15-25 minute.
10. according to an application for the biological inoculum nutritional agents described in any one of right 1-6,
It is characterized in that, the amount using described biological inoculum nutritional agents in sewage is 90-120g/m3。
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