CN102559498A - Nutritional agent for culturing microorganisms and microorganism culturing method - Google Patents
Nutritional agent for culturing microorganisms and microorganism culturing method Download PDFInfo
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- CN102559498A CN102559498A CN2011104575521A CN201110457552A CN102559498A CN 102559498 A CN102559498 A CN 102559498A CN 2011104575521 A CN2011104575521 A CN 2011104575521A CN 201110457552 A CN201110457552 A CN 201110457552A CN 102559498 A CN102559498 A CN 102559498A
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Abstract
The invention provides a nutritional agent for culturing microorganisms. The nutritional agent consists of the following components in parts by weight: 80-120 parts of glucose, 15-20 parts of urea, 2-5 parts of disodium hydrogen phosphate, 2-5 parts of monopotassium phosphate and 0.1-0.3 part of magnesium sulfate. The invention further provides a method for culturing organisms in sewage by using the nutritional agent. According to the nutritional agent and the microorganism culturing method provided by the invention, quick propagation of microorganisms in sewage can be promoted, adaption of microorganisms to the environment is accelerated, a large quantity of microorganisms needed in a sewage treatment process can be cultured within an extremely short period of time, and an optimal treatment effect is achieved.
Description
Technical field
The present invention relates to a kind of nutrition agent of culturing micro-organisms, be specifically related to the nutrition agent of culturing micro-organisms in a kind of sewage treatment process, and utilize this nutrition agent to cultivate method of microorganism in the sewage.
Background technology
In order to overcome the insufficient problem of mikrobe in the WWT initial stage water; At present general Sewage treatment systems all can have the debug phase of certain hour before formal operation; So that mikrobe shakes down and breeds in the water, the debug phase of general Sewage treatment systems is normal utilizes the outer mud that draws to start biochemical system, and this must bring following shortcoming: the anaerobic system start-up period is long; Load lifting is very slow, and the cultivation domestication time is generally 2～6 months; Aerobic system is under many factor affecting, and initial failure period also got more than 1 month, brings inconvenience for producer and engineering side, consumes a large amount of manpower and materials simultaneously.For the waste water of some high densitys, high ammonia nitrogen, high salinity, draw mud often need slowly cultivate domestication outward through former water is diluted, brought extra cost to engineering, prolonged start-up period, success ratio is low, can not satisfy the quick operation demand of system.For the waste water of biodegradability difference, as contain the waste water of materials such as benzene class, dyestuff, oils, conventional culturing sludge need add a large amount of external carbon sources, and speed is slow, and cost is high, and removal effect is undesirable, and difficulty up to standard is big.
Mikrobe reaches sufficient amount with the pollutent in disposing of sewage in the water in order to make; The contriver proposes a kind of mikrobe (bacterial classification) and cultivates used nutrition agent through constantly research, can promote the rapid breeding of mikrobe; Accelerate the adaptation of mikrobe to environment; In the extremely short time, can turn out needed mikrobe in a large amount of sewage treatment process, and make treatment effect reach best.
Summary of the invention
One object of the present invention is: a kind of nutrition agent that is used for culturing micro-organisms is provided; Can promote the rapid breeding of mikrobe in sewage; Accelerate the adaptation of mikrobe, in the extremely short time, can turn out needed mikrobe in a large amount of sewage treatment process environment.
Another object of the present invention is: a kind of microbial culture method is provided, can in the extremely short time, can turns out needed mikrobe in a large amount of sewage treatment process, and make treatment effect reach best.
Above-mentioned purpose of the present invention realizes through following technical scheme:
A kind of nutrition agent that is used for culturing micro-organisms is provided, forms: 80～120 glucose, 15～20 urea, 2～5 Sodium phosphate, dibasic, 2～5 potassium primary phosphate and 0.1～0.3 sal epsom by the component of following molar ratio.
Nutrition agent of the present invention preferably is made up of the component of following molar ratio: 100 glucose, 18 urea, 3 Sodium phosphate, dibasic, 3 potassium primary phosphate and 0.2 sal epsom.
Nutrition agent of the present invention can be mixed by commercially available various feed composition and get.
Said nutrition agent wherein, glucose can be microorganism growth carbon source is provided, urea can provide nitrogenous source, Sodium phosphate, dibasic and potassium primary phosphate and sal epsom can provide trace elements such as phosphorus and potassium, sodium, sulphur, magnesium.
The microbial growth breeding needs various nutrition.Aerobic microbiological colony the best requires BOD5 (C): N: P=100: 5: 1 (mol ratio); Anaerobion colony the best requires BOD5 (C): N: P=100: 6: 1 (mol ratio); Wherein, Anaerobion is more responsive to the variation of nutrition composition in the environment, and aerobic microbiological is lower to the change sensitivity of nutrition composition.The growth and breeding of comprehensive aerobic microbiological and anaerobion; In the nutrition agent prescription of the present invention; C: N: P=100: 5.8: 1 (mol ratio) all is in more satisfactory scope to the growth and breeding nutritional condition of aerobic microbiological and anaerobion; The growth and breeding of aerobic microbiological and anaerobion all is in comparatively ideal environment, helps the rapid breeding of aerobic microbiological and anaerobion most.
The present invention also provides method for culturing microbes in a kind of sewage, is at the operation of sewage disposal system initial stage, in sewage, adds the described nutrition agent of 150～300g/ ton; Under the envrionment conditions of routine, continuous aeration 20～30 hours is after continuous aeration is accomplished; Adopt the mode of intermittent aeration; Aeration 5～10 minutes stopped exposing to the sun 10～20 minutes, and so intermittent aeration is 8～12 days.
The preferred 250g/ ton of described nutrition agent dosage.
Preferred 24 hours of described continuous aeration time.
The preferred aeration of described intermittent aeration mode 7 minutes stopped exposing to the sun 14 minutes.
Preferred 9 days of described intermittent aeration time.
At system's initial operating stage, adopt cultural method of the present invention can promote the rapid breeding of aerobic microbiological in the sewage, facultative aerobic microbiological and anaerobion simultaneously.Compare with existing cultural method, this kind be cultural method efficiently, and incubation time microbe population short, that turn out is many, with strong points, can be widely used in various sewage treatment process.
Adopt microbial culture method of the present invention; Chinese Marine University's sanitary sewage disposal engineering, Zhong County, the Chongqing sanitary sewage disposal engineering of operation, Vietnam's cassava alcohol waste water treatment engineering, rice fragrance of a flower wastewater treatment project, the tenth of the twelve Earthly Branches sun clothing wastewater treatment project, luxuriant rich cashmere treatment of dyeing wastewater engineering; All reached best last effect in the short period of time, more traditional sludge acclimatization method time obviously shortens.
Embodiment
Embodiment 1.
A kind of nutrition agent that is used for cultivating the sewage mikrobe, form by the component of following molar ratio:
80 glucose, 20 urea, 2 Sodium phosphate, dibasic, 5 potassium primary phosphate and 0.3 sal epsom.
Above-mentioned each feed composition mixed promptly obtain nutrition agent of the present invention.
Embodiment 2.
A kind of nutrition agent that is used for cultivating the sewage mikrobe, form by the component of following molar ratio:
120 glucose, 15 urea, 5 Sodium phosphate, dibasic, 2 potassium primary phosphate and 0.1 sal epsom.
Above-mentioned each feed composition mixed promptly obtain nutrition agent of the present invention.
Embodiment 3.
A kind of nutrition agent that is used for cultivating the sewage mikrobe, form by the component of following molar ratio:
100 glucose, 18 urea, 3 Sodium phosphate, dibasic, 3 potassium primary phosphate and 0.2 sal epsom.
Above-mentioned each feed composition mixed promptly obtain nutrition agent of the present invention.
Embodiment 4.
Method for culturing microbes in a kind of sewage is at the operation of sewage disposal system initial stage, in sewage, adds the nutrition agent of 250g/ ton embodiment 3 preparations; Under the envrionment conditions of routine, continuous aeration 24 hours is after continuous aeration is accomplished; Adopt the mode of intermittent aeration; Aeration 7 minutes stopped exposing to the sun 14 minutes, and so intermittent aeration is 9 days.
Embodiment 5.
Method for culturing microbes in a kind of sewage is at the operation of sewage disposal system initial stage, in sewage, adds the nutrition agent of 300g/ ton embodiment 1 preparation; Under the envrionment conditions of routine, continuous aeration 20 hours is after continuous aeration is accomplished; Adopt the mode of intermittent aeration; Aeration 5 minutes stopped exposing to the sun 15 minutes, and so intermittent aeration is 12 days.
Embodiment 6.
The mikrobe of using microbial culture method of the present invention that Chinese ocean Dormitory on Campus is arranged in the sewage every day is cultivated:
Test used sewage and arranged sewage every day by the Chinese Marine University students' dormitory.Experiment is divided into two groups, and control group adopts traditional microbial acclimation method to cultivate, and experimental group adopts the cultural method of embodiment 4 to cultivate.Two groups of experiments are carried out under identical environment.In culturing process, carry out microscopy through microscope every day, analyzes the proterties of mud and the variation tendency of miniature organism kind and quantity.The result is following:
In the initial several days domestication process, protozoa peritricha (Peritricha) sessile form ciliate quantity has obvious minimizing, substitutes that big to measure existing be Holotricha (Holotricha) swimming type ciliate, and has the worms on a sucker of part to occur.Along with the propelling of incubation time, when the miniature organism in the mud adapted to new water quality and growing environment gradually, the ciliate of sessile form was restarted a large amount of appearance, and the companion has a spot of swimming type ciliate.In the culturing sludge later stage, the sessile form ciliate has comparative advantage, occurs miniature organisms such as roaming worm, wheel animalcule in succession, and this moment, the cultivation of mud was accomplished basically, and active sludge forms the macrobead floc sedimentation comprehensively, and structure is tight, and settling property is good.
Find through contrast: above-mentioned phenomenon appears in experimental group, and promptly the sessile form ciliate has comparative advantage, and when occurring miniature organism such as roaming worm, wheel animalcule in succession, the ciliate of control group sessile form begins a large amount of appearance, and companion has a spot of swimming type ciliate appearance.And when experiment proceeded to 10 days left and right sides, the characteristic of two experimental group mud reached the wherein situation such as the following table 1 of superior microorganism:
Table 1
Can find out that by above-mentioned experiment the microbial culture method of the present invention microbial numbers in the treatment system that can increase sharply is accelerated the adaptation of mikrobe to environment, thereby made treatment system in the extremely short time, just can reach best treatment effect.
Claims (7)
1. a nutrition agent that is used for culturing micro-organisms is characterized in that, it is made up of the component of following molar ratio: 80～120 glucose, 15～20 urea, 2～5 Sodium phosphate, dibasic, 2～5 potassium primary phosphate and 0.1～0.3 sal epsom.
2. the nutrition agent of the described culturing micro-organisms of claim 1 is characterized in that, it is made up of the component of following molar ratio: 100 glucose, 18 urea, 3 Sodium phosphate, dibasic, 3 potassium primary phosphate and 0.2 sal epsom.
3. method for culturing microbes in the sewage is characterized in that: at the operation of sewage disposal system initial stage, in sewage, add the described nutrition agent of 150～300g/ ton; Under the envrionment conditions of routine, continuous aeration 20～30 hours is after continuous aeration is accomplished; Adopt the mode of intermittent aeration; Aeration 5～10 minutes stopped exposing to the sun 10～20 minutes, and so intermittent aeration is 8～12 days.
4. the described cultural method of claim 3, it is characterized in that: described nutrition agent dosage is the 250g/ ton.
5. the described cultural method of claim 3, it is characterized in that: the described continuous aeration time is 24 hours.
6. the described cultural method of claim 3, it is characterized in that: described intermittent aeration mode is aeration 7 minutes, stops exposing to the sun 14 minutes.
7. the described cultural method of claim 3, it is characterized in that: the described intermittent aeration time is 9 days.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104150608A (en) * | 2014-07-31 | 2014-11-19 | 湖南永清水务有限公司 | Biochemical treatment process for high salinity wastewater |
| CN104419643A (en) * | 2013-09-03 | 2015-03-18 | 大连大孤山污水处理有限公司 | Culture process of novel biological flora |
| CN104893977A (en) * | 2015-05-13 | 2015-09-09 | 江苏恒丰科技有限公司 | Comprehensive microbe culture method |
| CN105779331A (en) * | 2016-03-07 | 2016-07-20 | 重庆坤泉环境工程有限公司 | Biological strain nutritional agent and preparation method and application thereof |
| CN106430555A (en) * | 2016-10-09 | 2017-02-22 | 北京恒蓝环保科学研究院有限责任公司 | Nutrient for aerobic treatment, aerobic treatment activated sludge culture method and device |
| CN106630180A (en) * | 2016-11-10 | 2017-05-10 | 中国海洋石油总公司 | Preparation method of slow release nutritional agent for increasing biochemical treatment effect of chemical industry production wastewater |
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| US4246100A (en) * | 1979-10-22 | 1981-01-20 | Bio-Humus, Inc. | Composition and method for the treatment of sewage |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104419643A (en) * | 2013-09-03 | 2015-03-18 | 大连大孤山污水处理有限公司 | Culture process of novel biological flora |
| CN104150608A (en) * | 2014-07-31 | 2014-11-19 | 湖南永清水务有限公司 | Biochemical treatment process for high salinity wastewater |
| CN104150608B (en) * | 2014-07-31 | 2016-03-09 | 湖南永清水务有限公司 | A kind of high-salt wastewater biochemical processing process |
| CN104893977A (en) * | 2015-05-13 | 2015-09-09 | 江苏恒丰科技有限公司 | Comprehensive microbe culture method |
| CN105779331A (en) * | 2016-03-07 | 2016-07-20 | 重庆坤泉环境工程有限公司 | Biological strain nutritional agent and preparation method and application thereof |
| CN106430555A (en) * | 2016-10-09 | 2017-02-22 | 北京恒蓝环保科学研究院有限责任公司 | Nutrient for aerobic treatment, aerobic treatment activated sludge culture method and device |
| CN106630180A (en) * | 2016-11-10 | 2017-05-10 | 中国海洋石油总公司 | Preparation method of slow release nutritional agent for increasing biochemical treatment effect of chemical industry production wastewater |
| CN106630180B (en) * | 2016-11-10 | 2019-05-28 | 中国海洋石油集团有限公司 | Improve the preparation method of the slow-release nutrient of chemical production wastewater Biochemical Treatment |
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| CN102559498B (en) | 2013-04-03 |
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