CN113200613A - Microbial nutrient solution for sewage treatment and preparation method and application thereof - Google Patents
Microbial nutrient solution for sewage treatment and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a microbial nutrient solution for sewage treatment and a preparation method and application thereof. The nutrient solution is prepared by the following raw materials through secondary fermentation: papaya, apple, mango and lemon. The preparation method of the microbial nutrient solution comprises the following steps: weighing fruits in proportion and cleaning; sterilizing, washing and dicing; juicing to obtain coarse pulp; carrying out aerobic fermentation on the coarse pulp to obtain a fermentation stock solution; mixing; anaerobic fermentation to obtain mixed fermentation liquor, and filtering to obtain filtrate; the filtrate was mixed with deionized water. The microbial nutrient solution can promote the rapid growth and proliferation of microorganisms for pollution treatment, thereby remarkably improving the efficiency of sewage treatment. The preparation method of the microbial nutrient solution is simple, the input cost is low, and batch production can be realized.
Description
Technical Field
The invention belongs to the technical field of microorganism application, and particularly relates to a microorganism nutrient solution for sewage treatment and a preparation method thereof.
Background
With the continuous promotion of the urbanization process and the increasing development of industrial economy, domestic sewage, industrial wastewater and the like in the urban area are more and more, the water quality is more and more complex, and the pollution is more and more serious. A large amount of domestic and industrial sewage flows into rivers and underground water, and urban sewage contains not only organic pollutants but also a large amount of suspended matters (including organic matters and inorganic matters), nitrogen and phosphorus pollutants and the like, and the pollutants have great negative effects on the daily life and the human health of human beings. The effective treatment and utilization of municipal sewage is a problem of general attention.
In recent years, the national attention and support for the environmental protection industry are continuously promoted, and a wide development space is provided for the urban sewage treatment market in China. Therefore, the demand for sewage treatment must be further expanded, and under the situation of increasing both sewage treatment rate and sewage discharge, the improvement of sewage treatment capacity is a trend and a necessary choice for water treatment industries and enterprises. The sewage treatment in China starts at a later time, and starts to appear at the end of the 20 th century and the 70 th era, the sewage treatment is limited by the conditions of technology, economy and the like at first, the proportion of a secondary sewage treatment plant is lower, most of the secondary sewage treatment plants mainly adopt organic processes such as an activated sludge method and the like, and the secondary sewage treatment plant is used for removing BOD and SS in the sewage; after the 21 st century, the new denitrification and dephosphorization process rises rapidly, the rapid growth becomes the mainstream process of sewage treatment, the proportion of BBR, A2/O, SBR, A/O and oxidation ditch processes gradually rises, and the new sewage treatment technology is better than the conventional activated sludge method in process and treatment effect. Wherein, the utilization of microorganisms to digest and decompose harmful substances in the sewage gradually becomes a new trend of harmless treatment of the sewage.
For example, CN105923773B discloses a multi-layer immobilized microorganism sewage treatment device, which combines immobilized microorganisms, aerobic and anaerobic properties of microorganisms and a magnetic field to realize sewage purification; CN110240291A discloses an ultra-shallow aeration microorganism biological bed and a sewage treatment system, wherein the biological bed comprises a bed body, a micropore aeration pipe, an auxiliary heating pipe, an overflow pipe, an air duct, a defoaming net and a microorganism carrier; CN108034602B discloses a microbial agent for heavy metal wastewater treatment, wherein the microbial agent has strong adsorption effect on heavy metals due to cellulolytic bacteria, nitrogen-fixing bacteria, fiber crushed materials and the like; CN102925357B discloses a composite microbial inoculum for harmless treatment, which comprises a plurality of bacteria and can be used for treating sewage, domestic garbage, sewage sludge or human and animal excreta.
Generally, the microbial treatment method is to consume nutrients in the sewage by using the growth and metabolism of microbes so as to decompose or reduce pollution elements and purify the water body. However, since sewage or sludge contains a large amount of indigenous microorganisms, how to make the microorganisms for sewage treatment grow and propagate into dominant species for a long time, and further promote the decomposition and removal of harmful pollutants in the sewage, and the realization of efficient purification of sewage is a key problem which needs to be solved.
Disclosure of Invention
The invention aims to provide a microbial nutrient solution for sewage treatment, which contains elements, amino acids and other nutrients required by growth and reproduction of microorganisms, and promotes the rapid growth of the microorganisms, particularly bacillus, streptomyces griseus and the like, so that the microbial agent is promoted to effectively remove excessive nitrogen and phosphorus, suspended matters and other pollutants in sewage.
The purpose of the invention is realized by the following technical scheme:
according to one aspect of the invention, the microbial nutrient solution for sewage treatment is prepared by sequentially carrying out aerobic fermentation and secondary fermentation of anaerobic fermentation on the following raw materials: papaya, apple, mango and lemon.
Wherein the microbial nutrient solution also comprises 0.5-2.5 wt% of amino acid based on the total weight of the microbial nutrient solution. The amino acid is selected from one or more of glutamic acid, arginine, proline, aspartic acid and phenylalanine. Preferably, the amino acids are selected from the group consisting of 1: (2-5) a combination of glutamic acid and phenylalanine.
More preferably, the microbial nutrient solution further comprises 1-1.5 wt% of amino acid based on the total weight of the microbial nutrient solution, and the amino acid taste is 1: 2-3 of glutamic acid and phenylalanine.
Wherein, the mass ratio of the pawpaw, the apple, the mango and the lemon is (1-6), (10-20), (5-12) and (2-8); preferably, the pawpaw, the apple, the mango and the lemon are mixed according to the mass ratio of (2-5) to (12-18) to (7-10) to (4-6).
Wherein, the raw materials of the microorganism nutrient solution also comprise: one or more of pineapple, grapefruit, and sweet orange. Preferably, the raw materials of the microbial nutrient solution also comprise pineapples, and the mass ratio of the pawpaw to the pineapples is 1: (1-5).
Preferably, the above fruits are selected from fruits in Hainan province.
Preferably, the fruit is selected from the group consisting of those having a ripeness of seven to eight ripeness. Wherein, the ripeness judgment of various fruits is carried out according to a method combining the external color and the fruit hardness commonly used in the prior art.
In the invention, the pawpaw contains abundant vitamins, various amino acids, K, Na, Ca, Mg and other elements; the apple is rich in carbohydrate, vitamins and nutrient elements such as Ca, Mn, Fe, Mo and I; the mango contains rich protein, vitamins, various amino acids, Fe, Mn, Mg and other elements; the lemon is rich in vitamin C, vitamin E and other vitamins, and is rich in elements such as P, Ca, Mg, Zn, Mn and the like; the pineapple fruit is rich in nutrition, and the pulp contains Ca, P, Fe and other elements besides saccharides, protein, fat and organic acid; the shaddock pulp is rich in nutrition and contains Ca, Mg, Fe, Cu and other elements; sweet orange contains a large amount of protein, dietary fiber, carbohydrate and vitamins, and various elements such as Ca, Fe, Zn, Si and the like.
According to another aspect of the present invention, there is provided a method for preparing a microbial nutrient solution, comprising:
(1) respectively weighing pawpaw, apple, mango and lemon according to a preset proportion, and cleaning;
(2) sterilizing, washing and dicing;
(3) juicing to obtain coarse pulp;
(4) carrying out aerobic fermentation on the coarse pulp of each fruit to obtain a fermentation stock solution;
(5) mixing the fermentation stock solutions of the fruits to obtain a mixed solution;
(6) carrying out anaerobic fermentation on the mixed solution to obtain mixed fermentation liquor, and filtering to obtain filtrate;
(7) and mixing the filtrate with deionized water to obtain the nutrient solution.
In the method, in the step (1), the washing means washing with water for 1-2 times to remove pesticides, pathogenic bacteria, soil, dust, impurities, etc. existing or remaining on the surface of the fruit.
In the method, in the step (1), papaya, apple, mango and lemon are mixed according to a mass ratio of (1-6) to (10-20) to (5-12) to (2-8). Preferably, the pawpaw, the apple, the mango and the lemon are mixed according to the mass ratio of (2-5) to (12-18) to (7-10) to (4-6).
In the method, in the step (2), the disinfection means that 30 to 50% (parts by mass) of an aqueous sodium chloride solution, or 0.1 to 0.2% (parts by mass) of an aqueous potassium permanganate solution, or 20 to 50mg/L of ClO2Sterilizing the water solution to remove pesticide, pathogenic bacteria and the like remained on the surface of the fruit.
In the method, in the step (2), the washing means washing the disinfected fruit 2-3 times with deionized water or pure water to remove the residual disinfection solution on the surface of the fruit.
In the method, in the step (2), the cut pieces mean pieces cut into a length of about 5 to 10 cm so as to facilitate a subsequent juicing process.
In the method, in the step (3), the juicing means juicing after removing peel of fruit pieces by using a grinder or a squeezer to obtain coarse pulp.
In the method, in the step (4), the aerobic fermentation comprises the steps of adding white sugar and/or brown sugar and yeast powder into the coarse pulp of each fruit, fermenting for 20-30 days at 32-38 ℃ in a sealed container, wherein the container cover is opened at intervals to ventilate once, and the mixture is stirred, mixed and sealed again. In the present invention, every other period means that aeration is performed every 1-2 days so that the fermentation broth has enough oxygen, which can ensure that the yeast cells are used to synthesize sterol and unsaturated fatty acid substances necessary for healthy cell membranes. Therefore, under aerobic conditions, yeast cells grow faster, reach higher cell density, and are beneficial to the fermentation of fruits.
Wherein the addition amount of the yeast powder is 2-8% of the weight of the coarse pulp.
Wherein the addition amount of white sugar and/or brown sugar is 5-10% of the weight of the coarse pulp.
In the method, in the step (6), the anaerobic fermentation comprises adding lactic acid bacteria powder, sealing the container, and continuously fermenting at 26-30 ℃ for 7-15 days. In the present invention, under anaerobic conditions, anaerobic fermentation results in the conversion of glucose to pyruvate, which is further converted to small molecules of lactate or ethanol that are readily digested by the microorganism.
In the method, in the step (6), the addition amount of the lactic acid bacteria powder is 1-5% of the weight of the mixed solution.
In the method, in the step (7), the mixed fermentation liquid is mixed with deionized water in a volume ratio of 1 (10-30). Preferably, the mixed fermentation broth is mixed with deionized water in a volume ratio of 1 (15-25).
Optionally, in step (8), amino acids are added such that the amount of amino acids added is 0.5-2.5 wt% based on the weight of the final nutritional liquid product.
According to another aspect of the present invention there is provided the use of a microbial nutrient solution according to the present invention for the treatment of wastewater to promote the growth and propagation of microorganisms in the wastewater environment.
In the use, the microorganism comprises a bacillus; preferably, the microorganism is a complex bacterium having bacillus as a main active bacterium. Wherein the Bacillus is selected from one or more of Bacillus subtilis (Bacillus subtilis), Bacillus licheniformis (Bacillus licheniformis), Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), Bacillus laterosporus (Brevibacillus laterosporus), Bacillus thuringiensis (Bacillus thuringiensis), Bacillus cereus (Bacillus cereus), Bacillus polymyxa (Bacillus polymyxa), Streptomyces griseus (Streptomyces griseus) and Thiobacillus denitrificus (Thiobacillus densificans).
Preferably, in the application, the microorganism is derived from a composite microbial agent, which comprises 10-20 parts of bacillus subtilis, 10-20 parts of bacillus licheniformis, 10-20 parts of bacillus amyloliquefaciens, 10-20 parts of bacillus laterosporus, 5-20 parts of streptomyces griseus and 5-10 parts of thiobacillus denitrificans.
Wherein the compound microbial agent also comprises a carbon source supplement, and the dosage of the carbon source supplement is 100-200 parts.
Wherein the carbon source supplement is starch and/or straw powder modified by weak base. The preparation method of the weak base modified straw powder comprises the following steps: taking the dried and crushed straw sieved by a sieve of 80-100 meshes, adding a weak base solution with the concentration of 10-20 wt%, uniformly mixing, pretreating for 48-72 hours at 40-55 ℃, then washing the pretreated straw with deionized water for 3-5 times, and drying. The weak base is selected from one or more of sodium carbonate, potassium carbonate, sodium bicarbonate, potassium bicarbonate, ammonia water or urea; the straws are selected from one or more of highland barley, corn, wheat, rice and cotton crops.
The microbial nutrient solution is prepared by mixing fermentation liquor of mango, apple, pawpaw, lemon and other fruit pulp with water, and the fruits are cleaned, sterilized, cut into blocks, squeezed and fermented to prepare the nutrient solution rich in elements such as potassium, magnesium, silicon, calcium, manganese and various amino acids. In the sewage treatment process, the nutrient solution can supplement nutrients required by growth and multiplication culture of bacillus and other bacteria to the maximum extent, promote the bacillus to form dominant flora in a local specific ecological system, better exert the microbial activity of the bacillus, and further improve the adsorption and decomposition of N, P and organic pollutants in sewage.
The technical scheme has the following advantages or beneficial effects:
the microbial nutrient solution prepared by the method has nutrients such as various nutrient elements, amino acids and the like which are necessary for the growth of various microorganisms, particularly the growth of bacteria such as bacillus and the like, so that the bacillus and the like can quickly become the dominant flora in a sewage treatment system in a short time; in addition, in the preparation method of the invention, by organically combining aerobic fermentation and anaerobic fermentation, the rapid accumulation of small molecular organic acid and ethanol which are easily consumed by bacillus and the like in the fermentation liquor is realized in a shorter fermentation period, so that the composite bacteria for pollution treatment can rapidly grow and proliferate, and the sewage treatment efficiency is further obviously improved.
In addition, the preparation method of the microbial nutrient solution is simple, the input cost is low, and batch production can be realized.
Drawings
Fig. 1 shows a schematic flow diagram of a process for preparing a microbial nutrient solution of the invention.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments, and it is obvious that the described embodiments are for illustrative purposes only, and not for all purposes. Based on the embodiments of the present invention, those skilled in the art will better understand and appreciate the technical solutions claimed in the present invention and the technical effects achieved thereby.
Each of the strains in the following examples was purchased from Beijing Beinan Chuangyi.
The biological rotating disk used in the embodiment of the invention is from Qinghai Jieshen environmental energy industry Co., Ltd, wherein the material of the biological rotating disk is polyvinylidene chloride (PVDC), the thickness is 6cm, the porosity is 98%, and the main working parameters are as follows: 30 disks/platform with the diameter of 2m are continuously operated to carry out sewage treatment, sewage comes from inlet water of urban sewage plants, and the treated water quantity is 2250m3Stage (d).
Preparation of nutrient solution
Example 1
(1) A total of 30kg of seven to eight times cooked papaya, apple, mango and lemon from southern Hainan of origin were mixed in a ratio of 3: 15: 7: 5, respectively weighing, and washing for 2 times by using water;
(2) soaking in 40 wt% sodium chloride water solution for 30 min for disinfection, washing with deionized water for 3 times, and cutting into fruit pieces with length of 5-10 cm;
(3) juicing the fruit blocks with peel removed by a squeezer to obtain coarse pulp;
(4) placing the coarse pulp of each fruit in a fermentation tank with a cover, adding white sugar 8% of the weight of the coarse pulp into the fermentation tank, adding yeast powder 5% of the weight of the coarse pulp, fermenting at 35 deg.C for 25 days, opening the container cover once a day, ventilating, stirring, mixing, sealing, and fermenting to obtain a fermentation stock solution;
(5) mixing the fermentation stock solutions of the fruits to obtain a mixed solution;
(6) placing the mixed solution in another sealed tank, adding 3 wt% of composite lactobacillus powder (Saiko biological science and technology Co., Ltd.) at 28 deg.C, sealing and fermenting for 10 days, and filtering to obtain filtrate;
(7) the filtrate was mixed with deionized water at a ratio of 1: 20, and mixing to obtain the nutrient solution.
The specific preparation process is shown in figure 1.
Example 2
The preparation raw materials and steps are the same as those in example 1, except that the mass ratio of the pawpaw, the apple, the mango and the lemon is 2: 10: 12: and 6. outside.
Example 3
The preparation raw materials and steps are the same as those in example 1, except that the mass ratio of the pawpaw, the apple, the mango and the lemon is 2: 10: 5: and 3. outside.
Example 4
The preparation raw materials and steps are the same as those in example 1, except that the mass ratio of the pawpaw, the apple, the mango and the lemon is 6: 15: 10: and 5, outside.
Example 5
The preparation raw materials and steps are the same as those in example 1, except that the mass ratio of the pawpaw, the apple, the mango and the lemon is 4: 16: 8: and 5, outside.
Example 6
The preparation of raw materials and other preparation steps were the same as in example 1 except that the fermentation temperature in step (4) was changed to 32 ℃ and the fermentation time was changed to 30 days.
Example 7
The preparation of the raw material and other preparation steps were the same as in example 1 except that the fermentation temperature in step (4) was changed to 38 ℃ and the fermentation time was changed to 21 days.
Example 8
The preparation of the raw material and other preparation steps were the same as in example 1 except that the fermentation temperature in step (6) was changed to 26 ℃ and the fermentation time was changed to 14 days.
Example 9
The preparation of raw materials and other preparation steps were the same as in example 1 except that the fermentation temperature in step (6) was changed to 30 ℃ and the fermentation time was changed to 8 days.
Example 10
The preparation of raw materials and other preparation steps were the same as in example 1 except that white sugar in an amount of 8% by weight of the brown syrup was changed to brown sugar in an amount of 5% by weight of the brown syrup in step (4), and yeast powder in an amount of 5% by weight was changed to 3%.
Example 11
The preparation of raw materials and other preparation steps were the same as in example 1 except that 8% by weight of white sugar in the brown stock was changed to 10% by weight of white sugar in the brown stock in step (4), and 5% by weight of yeast powder was changed to 8%.
Example 12
The preparation starting materials and preparation steps were the same as in example 1, except that phenylalanine was added to the final nutrient solution product at a final concentration of 1.4 wt% in step (7).
Example 13
The preparation raw materials and preparation steps were the same as in example 1 except that phenylalanine and glutamic acid were added to the final nutrient solution product in the final concentrations of 1 wt% and 0.4 wt%, respectively, in step (7).
Comparative example 1
The starting materials and other preparation steps were the same as in example 1, except that the anaerobic fermentation step not comprising step (6) was not included.
Comparative example 2
The preparation of the starting material and other preparation steps were the same as in example 1, except that the aerobic fermentation step not comprising step (4) was not included.
Comparative example 3
The procedure was as in example 1, except that the raw materials contained no apple.
Comparative example 4
The procedure was as in example 1, except that no lemon was included in the starting material.
Application example
Preparation of composite bacterial agent
1. Fermentation culture of each bacterium: respectively inoculating slant strains of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus laterospora and thiobacillus denitrificans into LB liquid culture medium, and respectively culturing at 37 ℃; inoculating slant strain of Streptomyces griseus into fermentation medium (soybean cake powder 5%, corn starch 4%),Ammonium sulfate 0.6%, K2HPO4 1％、MgSO4·7H20.075 percent of O, 1.0 percent of NaCl, and pH7.8 (refer to Lihaiyan and the like, science, technology and engineering, 2013, 13(27), 8202 and 8204)), and culturing at 32 ℃ until the concentration of viable bacteria of each bacterium in the liquid fermentation broth is not less than 1 x 109And (5) CFU/ml, and freeze-drying to obtain the dormant fungus dry powder.
2. Preparing modified straw powder: taking the dried and crushed highland barley straws screened by a 100-mesh sieve, adding ammonia water with the concentration of 14 percent, which is 10 times of the mass of the straws, uniformly stirring, pretreating at 50 ℃ for 48 hours, then washing the pretreated straws with deionized water for 3 times, and drying.
3. Mixing the bacteria, the starch and the modified highland barley dry powder according to the following proportion to obtain the compound microbial agent of the invention:
15 parts of bacillus subtilis, 15 parts of bacillus licheniformis, 15 parts of bacillus amyloliquefaciens, 15 parts of bacillus laterosporus, 13 parts of streptomyces griseus, 8 parts of thiobacillus denitrificans, 100 parts of starch and 50 parts of modified highland barley straw powder.
(II) sewage treatment
1) Domestic sewage from municipal pipelines (raw water: COD 277mg/L, TN 58mg/L, TP 6.3mg/L, NH3-N ═ 45mg/L) through a coarse grid well and a fine grid well in sequence to remove large volumes of pollutants, wherein the mesh of the coarse grid is 20mm and the mesh of the fine grid is 3 mm;
2) the effluent of the fine grid well flows into a grit chamber to precipitate other impurities in the water;
3) the effluent of the grit chamber enters a mixing tank, and the total volume of the effluent of the grit chamber relative to the total volume of the influent of the mixing tank is 0.06g/m3D, adding the nutrient solution prepared in the embodiment and the comparative example in an amount which is 300 times that of the original nutrient solution, and continuously adding the nutrient solution for 24 hours;
4) the effluent of the mixing tank enters an anaerobic tank, a three-dimensional rotating biological disk is arranged in the anaerobic tank, wherein 50 percent of the rotating biological disk is immersed in the sewage, DO is 0.2mg/L, and the rotating biological disk rotates at a constant speed of 4 revolutions per minute;
5) the effluent of the anaerobic tank flows into a biochemical tank relative to the total inflow of the biochemical tankVolume at 100g/m3Adding the composite microbial inoculum prepared in the step (I), supplying air to the biochemical pool through an air blower, adjusting an air pipe valve to keep the dissolved oxygen amount in the biochemical pool at 2.5mg/L, setting a mixed liquid reflux pump of the effluent of the biochemical pool at the tail end of the biochemical pool, and refluxing to a mixed water distribution pool, wherein the reflux amount is 1Q;
6) effluent in the biochemical tank flows into a secondary sedimentation tank, sludge and water are separated in the secondary sedimentation tank, part of sludge in the secondary sedimentation tank flows back into the anaerobic tank through a sludge reflux pump, and the reflux quantity is 1Q;
7) and the effluent of the secondary sedimentation tank flows into the disinfection system and is discharged through the effluent metering tank.
The COD (chemical oxygen demand), TN, TP and NH3-N (ammonia nitrogen) of the treated sewage are respectively measured by adopting industrial or national standards. Wherein, the COD is measured by a potassium dichromate method (GBT 11914-89), the total N is measured by an alkaline potassium persulfate digestion ultraviolet spectrophotometry method (GB11894-89), the total P is measured by an ammonium molybdate spectrophotometry method (GB 11893-89), and the ammonia nitrogen is measured by a gas phase molecular absorption spectrometry method (HJ/T195-2005).
The measurement results are shown in table 1 below:
TABLE 1
As can be seen from Table 1, COD, TN, TP and NH of the nutrient solution treatment with addition of both aerobic and anaerobic fermentations were compared with the nutrient solution without aerobic fermentation or anaerobic fermentation process3The removal rate of-N is remarkably reduced, but the effect on the removal of N is relatively large, which fully explains that the aerobic fermentation and anaerobic fermentation processes of fruits are very important for the growth and proliferation of various bacteria. Various small molecular organic acids and alcohols generated in the fermentation process promote bacillus and the like in the sewage to quickly become dominant strains, so that the excess N, P component in the sewage and the removal of organic matters are obviously improved. As shown in comparative examples 3-4, nutrient treatment without papaya or without citric acid had significant TP and COD removal compared to the nutrient solutions of the present inventionThe influence may be caused because the growth conditions of bacillus and the like are influenced by major elements or trace elements contained in pawpaw and citric acid, and the removal effect of P and organic pollutants in sewage is further influenced.
In addition, tests on the nutrient solution prepared by the invention show that the viable bacteria concentration in the sewage is respectively measured by sampling from the mixed distribution tank at 4 th, 8 th, 12 th, 16 th, 20 th, 24 th, 36 th and 48 th hours after the compound microbial inoculum is added into the mixed distribution tank, and as a result, the nutrient solution has the following results: the viable bacteria concentration of the complex microbial inoculum treated by adding the nutrient solution of the invention is steadily increased within 24 hours, for example, the viable bacteria concentration of the nutrient solution prepared in example 1 in 24 hours reaches 4.5X 109CFU/ml or more, and is maintained at 6.5X 10 in the following 48 hours9CFU/ml or more, and the viable bacteria concentration reached 4.5X 10 at 20 hours after the treatment with the phenylalanine-containing nutrient solution prepared in example 129CFU/ml is above; the viable cell concentration reached 4.5X 10 at 18 hours after the treatment with the amino acid-containing nutrient solution prepared in example 139CFU/ml above. The amino acid, particularly the compound phenylalanine and glutamic acid, added into the nutrient solution provided by the invention can generate a synergistic compounding effect, so that the growth and propagation speed of the compound bacteria can be obviously increased, and the nutrient solution is more beneficial to sewage treatment.
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Various alternatives, modifications and combinations of the features of the invention can be made without departing from the spirit and nature of the invention as claimed, and such simple variations and combinations should also be considered as disclosed in the present application, all falling within the scope of the invention.
Claims (10)
1. The microbial nutrient solution for sewage treatment is characterized by being prepared from the following raw materials by aerobic fermentation and anaerobic fermentation in sequence: papaya, apple, mango and lemon.
2. The microbial nutrient solution of claim 1, further comprising 0.5-2.5 wt% of amino acid based on the total weight of the microbial nutrient solution, wherein the amino acid is selected from one or more of glutamic acid, arginine, proline, aspartic acid and phenylalanine; preferably, the amino acid is selected from the group consisting of glutamic acid and phenylalanine; more preferably 1: (2-5) a combination of glutamic acid and phenylalanine.
3. The microbial nutrient solution of claim 2, wherein the raw materials of the microbial nutrient solution further comprise: one or more of pineapple, grapefruit, and sweet orange.
4. The microbial nutrient solution of claim 1, wherein the mass ratio of the pawpaw, the apple, the mango and the lemon is (1-6): (10-20): (5-12): (2-8); preferably, the mass ratio of the pawpaw, the apple, the mango and the lemon is (2-5): (12-18): (7-10): (4-6).
5. The microbial nutrient solution of claim 1, wherein the aerobic fermentation is a raw pulp obtained by juicing each fruit, adding white sugar and/or brown sugar and yeast powder, and fermenting in a sealed container at 32-38 ℃ for 20-30 days; the anaerobic fermentation is carried out by adding lactobacillus powder, sealing the container, fermenting continuously at 26-30 deg.C for 7-15 days, and fermenting at 32-38 deg.C for 20-30 days.
6. A preparation method of a microbial nutrient solution for sewage treatment comprises the following steps:
(1) respectively weighing pawpaw, apple, mango and lemon according to a preset proportion, and cleaning;
(2) sterilizing, washing and dicing;
(3) juicing to obtain coarse pulp;
(4) carrying out aerobic fermentation on the coarse pulp to obtain a fermentation stock solution;
(5) mixing the fermentation stock solutions of the fruits to obtain a mixed solution;
(6) carrying out anaerobic fermentation on the mixed solution to obtain mixed fermentation liquor, and filtering to obtain filtrate;
(7) and mixing the filtrate with deionized water to obtain the nutrient solution.
7. The production method according to claim 6, wherein in the step (1), papaya, apple, mango and lemon are weighed in a mass ratio of (1-6): (10-20): (5-12): (2-8); preferably, the papaya, apple, mango and lemon are weighed in a mass ratio of (2-5): (12-18): (7-10): (4-6).
8. The method according to claim 6, wherein in the step (4), the aerobic fermentation comprises the steps of: adding white sugar and/or brown sugar and yeast powder into the coarse pulp, fermenting at 32-38 deg.C in a sealed container for 20-30 days, periodically opening the container cover, ventilating, stirring, mixing, and sealing; in the step (6), the anaerobic fermentation comprises adding lactobacillus powder, sealing the container, and continuously fermenting at 26-30 ℃ for 7-15 days.
9. The method according to claim 8, wherein the white sugar and/or brown sugar is added in an amount of 5-10% by weight of the brown syrup, and the yeast powder is added in an amount of 2-8% by weight of the brown syrup.
10. Use of a microbial nutrient solution according to any one of claims 1-5 or prepared according to the preparation method of any one of claims 6-9 for promoting the growth and reproduction of microorganisms in a sewage environment, preferably, the microorganisms are complex bacteria of bacillus as a major active bacteria.
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Cited By (2)
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