CN112980741A - Compound microbial agent for sewage treatment and preparation method and application thereof - Google Patents
Compound microbial agent for sewage treatment and preparation method and application thereof Download PDFInfo
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- CN112980741A CN112980741A CN202110433855.3A CN202110433855A CN112980741A CN 112980741 A CN112980741 A CN 112980741A CN 202110433855 A CN202110433855 A CN 202110433855A CN 112980741 A CN112980741 A CN 112980741A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
Abstract
The invention relates to a compound microbial agent for sewage treatment and a preparation method and application thereof. The microbial inoculum is prepared from the following raw materials: bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens, bacillus laterosporus, streptomyces griseus and thiobacillus denitrificans, and optionally weak base modified straw powder. The preparation of the microbial compound inoculant comprises the following steps: respectively preparing dormant microorganism dry powder, optionally adding weak base modified straw powder, and mixing according to a preset proportion to obtain the composite microbial inoculum. The eutrophic sewage treated by the composite microbial agent has the following beneficial effects: the strain of the compound microbial agent as a dominant strain has long duration, long survival time, high organic matter removal efficiency, simple preparation method, less varieties of required strains and low input cost, can realize batch production, and can be used for large-scale sewage treatment application.
Description
Technical Field
The invention belongs to the technical field of microorganism application, and particularly relates to a compound microorganism bacterium agent for sewage treatment and a preparation method and application thereof.
Background
With the continuous promotion of the urbanization process and the increasing development of industrial economy, domestic sewage, industrial wastewater and the like in the urban area are more and more, the water quality is more and more complex, and the pollution is more and more serious. A large amount of domestic and industrial sewage flows into rivers and underground water, and urban sewage contains not only organic pollutants but also a large amount of suspended matters (including organic matters and inorganic matters), nitrogen and phosphorus pollutants and the like, and the pollutants have great negative effects on the daily life and the human health of human beings. The effective treatment and utilization of municipal sewage is a problem of general attention.
At present, people try to adopt various physical, chemical or biological modes to treat sewage, strive to reduce the content of various pollutants in urban water bodies, and thus improve the water quality of the water bodies. For example, CN110902849B discloses a method for treating industrial wastewater by using a biological adsorbent, wherein ZrO2-Fe3O4 is used as a magnetic nano-absorbent, and biological carriers such as chitosan, sodium alginate, talc powder and the like are added; CN1303009C discloses a clean integrated sewage treatment system, which is a three-dimensional closed system consisting of three reaction zones to purify sewage; CN109701311A discloses a sewage treatment device convenient for maintenance, in which a connecting cylinder and a purifying cylinder can be detached, so that a filter screen inside the connecting cylinder can be easily cleaned, the filter screen is prevented from being blocked, and higher purifying efficiency is realized; CN108421423A discloses a photocatalytic sewage treatment composite membrane for sewage treatment, which increases the photocatalytic degradation of pollutants in wastewater by increasing the contact area of photocatalysis; CN112079474A discloses a sewage treatment process for sludge reduction, which adopts chemical measures such as filtration, flocculation and COD remover to reduce the sludge content in sewage and the factors generating sludge, thereby improving the sewage treatment effect; CN108034602B discloses a microbial agent for heavy metal wastewater treatment, wherein the microbial agent has strong adsorption effect on heavy metals due to cellulolytic bacteria, nitrogen-fixing bacteria, fiber crushed materials and the like. Compared with the traditional physical and chemical treatment mode, the biological mode, especially the treatment mode for treating the sewage through the metabolism of the microorganisms is an environment-friendly treatment mode with low cost and difficult secondary pollution.
The conventional microorganism treatment method is to consume nutrient substances in the sewage by utilizing the growth and metabolism of microorganisms so as to achieve the purposes of decomposing or reducing pollution elements and purifying water bodies. However, the growth of microorganisms must satisfy a certain carbon-nitrogen ratio, and when the carbon source in the wastewater is insufficient or is exhausted by the microorganisms, or when the carbon source in the water is excessive and causes N deficiency, the growth and metabolic activity of the microorganisms are affected, and the effect of wastewater treatment is affected. In addition, because the components of the current urban polluted water are complex and the pollutants are diversified, the effective purification effect of the single microbial strain for treating the sewage is difficult to realize.
Therefore, in the current situation of complex urban sewage, how to utilize the growth and metabolism of microorganisms is an urgent problem to be solved.
Disclosure of Invention
The invention aims to provide a compound microbial agent for sewage treatment, which is derived from various activated sludge bacteria of activated sludge, and can effectively remove excessive nitrogen and phosphorus, suspended matters and other pollutants in sewage through reasonable compatibility.
The purpose of the invention is realized by the following technical scheme:
according to one aspect of the invention, the compound microbial agent for sewage treatment is provided, which is prepared from the following raw materials: bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus laterosporus, Streptomyces griseus and Thiobacillus denitrificans.
The compound microbial agent is prepared from the following raw materials in parts by weight: 10-20 parts of bacillus subtilis, 10-20 parts of bacillus licheniformis, 10-20 parts of bacillus amyloliquefaciens, 10-20 parts of bacillus laterosporous, 5-20 parts of streptomyces griseus and 5-10 parts of thiobacillus denitrificans.
Wherein, the content of the bacillus subtilis is 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and 20 parts by weight or any value in any range between the parts.
Wherein the content of the bacillus licheniformis is 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and 20 parts by weight or any value in any range between the two parts.
Wherein the content of the bacillus amyloliquefaciens is 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and 20 parts by weight or any value in any range between the parts.
Wherein the content of the lateral cytoblast bacillus is 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and 20 parts by weight or any value in any range between the two parts.
Wherein, the content of the streptomyces griseus is 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 parts by weight or any value in any range between the two parts.
Wherein, the content of the denitrobacterium is 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10 parts by weight or any value in any range between the two parts.
Wherein, the raw materials of the compound microbial agent also comprise a carbon source supplement, and the dosage of the carbon source supplement is 100-200 parts.
Wherein the carbon source supplement is starch and/or straw powder modified by weak base. Wherein the weak base is selected from one or more of sodium carbonate, potassium carbonate, sodium bicarbonate, potassium bicarbonate, ammonia water or urea; the straws are selected from one or more of highland barley, corn, wheat, rice and cotton crops.
Preferably, the carbon source supplement is prepared by compounding starch and weak base modified straw powder according to the mass ratio of 1-2: 1-2.
The preparation method of the weak base modified straw powder comprises the following steps: taking the dried and crushed straw sieved by a sieve of 80-100 meshes, adding a weak base solution with the concentration of 10-20% (mass fraction), uniformly mixing, pretreating at 40-55 ℃ for 48-72 hours, then washing the pretreated straw with deionized water for 3-5 times, and drying.
Preferably, the straw powder is modified by ammonia water. The preparation method of the ammonia water modified straw powder comprises the following steps: taking the dried and crushed straw sieved by a sieve of 80-100 meshes, adding ammonia water with the concentration of 12-16%, uniformly mixing, pretreating for 48-72 hours at 40-55 ℃, then washing the pretreated straw with deionized water for 3-5 times, and drying.
Wherein, the raw materials of the compound microbial inoculum also comprise: one or more of Bacillus thuringiensis (Bacillus thuringiensis), Bacillus cereus (Bacillus cereus) and Bacillus polymyxa (Bacillus polymyxa).
Wherein, the raw materials of the compound microbial inoculum also comprise: bacillus amyloliquefaciens, bacillus cereus and bacillus polymyxa. Wherein, the composite microbial agent comprises the following raw materials in parts by weight: 1-5 parts of bacillus amyloliquefaciens, 1-5 parts of bacillus cereus and 1-5 parts of bacillus polymyxa.
According to another aspect of the present invention, there is provided a method for preparing a complex microbial inoculant for sewage treatment, comprising the following steps:
1) respectively inoculating slant strains of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus laterospora, Streptomyces griseus and Thiobacillus denitrificans into respective suitable liquid culture medium, optionally adding at least one of Bacillus amyloliquefaciens, Bacillus cereus and Bacillus polymyxa, and performing liquid fermentation culture until the viable bacteria concentration of each strain in the liquid fermentation liquid is not less than 1 × 109CFU/ml, dehydrating and drying to obtain dormant microorganism dry powder;
2) and mixing the prepared dormant microorganism dry powder according to a preset proportion to obtain the compound microorganism bacterium agent.
Preferably, in step 2), a carbon source supplement is added. Specifically, the prepared dormant microbial dry powder and a carbon source supplement are mixed according to a preset proportion to obtain the compound microbial agent.
Wherein, the bacillus subtilis, the bacillus licheniformis, the bacillus amyloliquefaciens, the bacillus laterospora, the streptomyces griseus, the thiobacillus denitrificans, the bacillus thuringiensis, the bacillus cereus and the bacillus polymyxa can be cultured by liquid fermentation by utilizing the technology known in the field.
According to still another aspect of the present invention, there is provided a method for sewage treatment, comprising the steps of:
1) removing large-volume pollutants from domestic sewage from municipal pipelines by sequentially passing through a coarse grating well and a fine grating well, wherein meshes of the coarse grating are 25-100mm, and meshes of the fine grating are 1.5-25 mm;
2) the effluent of the fine grid well flows into a grit chamber to precipitate other impurities in the water;
3) the effluent of the grit chamber enters an anaerobic tank;
4) the effluent of the anaerobic tank enters a mixed distribution tank, and the total volume of the effluent of the anaerobic tank is 15g/m relative to the total volume of the influent of the mixed distribution tank3D, adding microbial nutrients containing silicon and magnesium, and fully mixing, wherein a three-dimensional biological rotating disc is arranged in the mixing distribution pool, 40-50% of the area of the biological rotating disc is immersed in sewage, and DO (total dissolved oxygen) is 0.2-1mg/L and rotates at a constant speed of 2-5 revolutions per minute;
5) enabling the effluent of the mixed distribution pool to flow into a biochemical pool, supplying air into the biochemical pool through an air blower, and adjusting the dissolved oxygen in the biochemical pool to keep 0.2-2.0mg/L, wherein the mixed liquid in the biochemical pool flows back into the mixed distribution pool, and the backflow amount is 0-1Q;
6) effluent in the biochemical tank flows into a secondary sedimentation tank, mud and water in the secondary sedimentation tank are separated, a part of sludge in the secondary sedimentation tank flows back to the anaerobic tank, and the backflow amount is 0-1Q;
7) and the effluent of the secondary sedimentation tank flows into the disinfection system and is discharged through the effluent metering tank.
Wherein, the compound microbial agent is added into a biochemical pool.
According to another aspect of the present invention, there is provided a use of the complex microbial inoculant described above in sewage treatment, particularly in eutrophic sewage treatment.
In the invention, various bacteria cooperate with each other to play a role in purifying water quality together.
The bacillus subtilis has strong adaptability and can secrete various digestive enzymes, particularly amylase, lipase, cellulase and the like, so that various organic matters can be decomposed. In the invention, active substances such as subtilin, polymyxin, zymostatin, amylase, protease and the like secreted by bacillus subtilis can inhibit the growth and propagation of harmful pathogenic bacteria (such as vibrio, escherichia coli) and the like in water, so that various organic pollutants in sewage can be decomposed, and the aim of reducing the organic pollutants is fulfilled.
Bacillus licheniformis is a bacterium with strong enzymatic activity. In the invention, under the condition of oxygen, bacillus licheniformis secretes amylase, protease, lipase and cellulase, thereby decomposing organic substances such as protein, cellulose, starch, lipid and the like in sewage. Under the anoxic condition, the bacillus licheniformis secretes nitrate reductase to reduce nitrate into nitrite, and then nitrogen is generated. In addition, Bacillus licheniformis may also produce active substances that inhibit the growth and reproduction of pathogenic bacteria.
Bacillus amyloliquefaciens is an important phosphate solubilizing bacterium, and can secrete phosphate solubilizing enzyme to change solid phosphorus into ionic phosphorus. In the invention, the bacillus amyloliquefaciens can enable the particles, heavy metal ions or virus particles suspended in the sewage to be connected into groups through active functional groups such as hydroxyl, carboxyl and the like on the polysaccharide or protein through chemical reaction, hydrogen bonds, static electricity and the like by secreting, chitinase and other polysaccharides and proteins with flocculation functions, thereby accelerating the coagulation and sedimentation of the suspended matters and purifying the water quality.
Bacillus laterosporus is a type of aerobic Bacillus cereus, can be used as a low-toxicity insecticide of microbial origin, and can produce two major types of toxins, namely endotoxin and exotoxin. In the present invention, the reason why the Bacillus laterosporus purifies the sewage may be that its nutrients produce endotoxin and exotoxin in the course of becoming a spore state, which causes the death of harmful bacteria in the sewage.
Streptomyces griseus is an actinomycete which can produce various antibiotics such as streptomycin, terramycin and the like, has developed mycelium and has strong starch and protein hydrolysis capacity. In the present invention, Streptomyces griseus produces a large amount of hyphae, which not only have an adsorption effect, but also produce protease, amylase, cellulase and other substances, effectively decompose various organic substances in sewage, and decompose cellulose as a carbon source for growth and propagation of various bacteria. In addition, a large amount of hyphae produced by the streptomyces griseus can adsorb other bacteria to form active bacteria mass, so that the decomposition of various pollutants in the sewage is accelerated.
Thiobacillus denitrificans is an important functional bacterium in the denitrification process. In the present invention, under anoxic conditions, Thiobacillus denitrificans uses sulfide as electron donor and NO in nitrate3 -As an electron acceptor, an oxidation-reduction reaction proceeds to release nitrogen (N)2) Or dinitrogen monoxide (N)2O) and removing the sulfide in the sewage.
The main components of the straw are cellulose, hemicellulose and lignin, which are rich in a plurality of active functional groups such as hydroxyl, carbonyl, carboxyl and the like. In the invention, the sewage purification effect is improved by modifying the straws with alkali, and the reason may be that the roughness of the surface of the straws after the alkali treatment is increased, so that cellulase and hemicellulase produced by various bacilli, streptomyces griseus and the like in the composite microbial inoculum can more easily contact and decompose straw cellulose, and finally glucose is generated to serve as a supplementary carbon source of various bacteria. The effect is better when the starch is matched for use.
The technical scheme has the following advantages or beneficial effects:
in the invention, various components in the composite microbial inoculum act synergistically. Particularly, under the action of adsorption bearing of straw powder and mucus secreted by various spore bacteria, various active bacteria or active bacteria groups are more easily and uniformly dispersed in sewage or adsorbed on a high-carbon-source, long-mud-age biological rotating disc. Bacillus subtilis, Bacillus licheniformis, Streptomyces griseus and the like generate cellulase, and cellulose is decomposed to generate glucose so as to provide necessary carbon substrates for the growth of various active bacteria, especially under the condition of low C/N ratio; the flocculation component secreted by the giant bacillus and the meristematic hypha donor of the streptomyces griseus promote various bacteria to form larger active bacterial clusters, so that the bacillus griseus not only has stronger biological flocculation and adsorption capacity and the capacity of oxidizing and decomposing various pollutants, but also provides a good living environment for various beneficial microorganisms in sewage; under the action of toxins or antibiotics secreted by bacillus and streptomyces griseus, most of viruses and pathogenic bacteria in the sewage are killed, and the growth and reproduction environments of various active bacteria are further improved; on the premise of sufficient substrate and good growth environment, various nitrifying bacteria, denitrifying bacteria and sulphide bacteria can better digest and decompose pollutants such as N, P, S, and the like, so that the water quality of the sewage is purified.
Compared with indigenous strains of sewage or sludge species, the strains of the composite microbial agent, particularly bacillus, can quickly become dominant strains and have long survival time, and can continuously and stably reduce BOD, COD, TN, TP, nitrate and heavy metal contents of the sewage at a low C/N ratio, so that the removal efficiency of organic matters is high, and the purification of eutrophic water is efficiently realized. In addition, the preparation method of the compound microbial agent is simple, the variety of the required strains is few, the investment cost is low, the mass production can be realized, and the compound microbial agent is used for large-scale sewage treatment application.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments, and it is obvious that the described embodiments are for illustrative purposes only, and not for all purposes. Based on the embodiments of the present invention, those skilled in the art will better understand and appreciate the technical solutions claimed in the present invention and the technical effects achieved thereby.
Each of the strains in the following examples was purchased from Beijing Beinan Chuangyi.
The biological rotating disk used in the embodiment of the invention is from Qinghai Jieshen environmental energy industry Co., Ltd, wherein the material of the biological rotating disk is polyvinylidene chloride (PVDC), the thickness is 6cm, the porosity is 98%, and the main working parameters are as follows: 30 disks with the diameter of 1m are continuously operated to carry out sewage treatment, sewage comes from inlet water of urban sewage plants, and the treated water quantity is 3000m3/d。
Preparation of composite bacterial agent
1. Preparation of culture Medium
1.1, seed culture medium: peptone 1%, yeast extract 0.5%, sodium chloride 1%, and natural pH;
1.2, basic fermentation medium: soybean cake powder 5%, corn starch 4%, ammonium sulfate 0.6%, K2HPO4 1％、MgSO4·7H2O 0.075％，NaCl 1.0％、pH7.8；
2. Activating strains: transferring the preserved strain to a slant culture medium for culture for later use;
3. preparation of seed liquid
Inoculating activated strain into 250ml triangular flask containing 50ml seed culture medium at 37 deg.C
Culturing at 180r/min for 18 h;
4. shaking culture
Respectively taking 1ml of seed solution, inoculating into a 250ml triangular flask containing 50ml of fermentation medium, and performing shake culture at 30 ℃ for 12h at the rotating speed of 160 r/min;
5. conditions of fermentation
Adopting a bacterial liquid cultured in a shake flask at the late stage of logarithmic phase as a seed liquid to be inoculated into a fermentation tank, and adjusting initial pH and temperature, liquid loading amount, inoculation amount, ventilation amount, foam, thallus concentration, matrix, fermentation time and the like before fermentation;
6. fermentation process
1) Mixing the raw materials of the fermentation medium in proportion in a proportioning tank, uniformly stirring, adding a proper amount of water, and mixing with the prepared dry materials to form slurry;
2) steam sterilization is carried out on all pipelines, sterilization tanks, fermentation tanks and other series facilities, and the sterilization time is determined according to the situation;
3) after the fermentation medium is sterilized, because the medium is at a high temperature during sterilization, the medium is cooled by a spray cooling pipe (if the high-temperature medium is directly conveyed into a fermentation tank for cooling, a large amount of time is needed), and then the medium is conveyed to the fermentation tank through a pipeline;
4) the air filtration system is used for carrying out multi-stage filtration and steam sterilization on the air, and the air compressor sends sterile air into the seed tank and the fermentation tank through pipelines;
5) taking the bacterial liquid at the last stage of logarithmic phase as seed liquid, inoculating the seed liquid into a first-stage seed tank through a pipeline or an inoculation port, then inoculating the seed liquid into a second-stage seed tank from the first-stage seed tank, and then inoculating the seed liquid into a fermentation tank from the second-stage seed tank;
6) conveying the fermented product to a secondary fermentation tank for fermentation after fermentation by a primary fermentation tank according to the fermentation amount, and controlling the pH, the temperature, the liquid loading amount, the inoculation amount, the ventilation amount, the foam, the thallus concentration, the substrate quality and other influence factors by utilizing a detection system to regulate and control parameters in time during the fermentation period so as to ensure that the fermentation production is well controllable;
7) the fermentation period is generally 6-7 days, and the fermentation time of different strains is different;
8) conveying the fermented liquid to a filtering and concentrating device through a pipeline to further filter and concentrate the fermented liquid;
9) conveying the bacillus subtilis solution to a drying workshop through a pipeline, and drying the concentrated solution by adopting a spray drying device to prepare bacillus powder;
10) the single bacillus powder is mixed and stirred evenly according to the preparation proportion, and finally the composite bacillus agent is prepared.
Example 1
1. Fermentation culture of each bacterium: respectively inoculating slant strains of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus laterospora and thiobacillus denitrificans into LB liquid culture medium, and respectively culturing at 37 ℃; inoculating slant strain of Streptomyces griseus into fermentation culture medium (soybean cake powder 5%, corn starch 4%, ammonium sulfate 0.6%, K)2HPO4 1％、MgSO4·7H2O0.075%, NaCl 1.0%, pH7.8 (refer to Lissian, etc., family)Chemical technology and engineering, 2013, 13(27), 8202-8204)), at 32 ℃ until the viable bacteria concentration of each bacterium in the liquid fermentation broth is not less than 1 × 109And (5) CFU/ml, and freeze-drying to obtain the dormant fungus dry powder.
2. Preparing modified highland barley straw powder: taking the dried and crushed highland barley straws screened by a 100-mesh sieve, adding ammonia water with the concentration of 14 percent (mass fraction) which is 10 times of the mass of the straws, uniformly stirring, pretreating at 50 ℃ for 48 hours, then washing the pretreated straws with deionized water for 3 times, and drying.
3. Mixing the bacteria and the modified highland barley dry powder according to the following proportion to obtain the compound microbial agent of the invention:
15 parts of bacillus subtilis, 15 parts of bacillus licheniformis, 15 parts of bacillus amyloliquefaciens, 15 parts of bacillus lateraporus, 13 parts of streptomyces griseus, 8 parts of thiobacillus denitrificans, 100 parts of starch and 50 parts of modified highland barley straw powder.
Example 2
Each dry powder of the microorganism in a dormant state was prepared in the same manner as in example 1, and the respective components were mixed in the following proportions to obtain a complex microorganism bacterium agent.
15 parts of bacillus subtilis, 15 parts of bacillus licheniformis, 15 parts of bacillus amyloliquefaciens, 15 parts of bacillus lateraporus, 13 parts of streptomyces griseus, 8 parts of thiobacillus denitrificans, 75 parts of starch and 75 parts of modified highland barley straw powder.
Example 3
Each dry powder of the microorganism in a dormant state was prepared in the same manner as in example 1, and the respective components were mixed in the following proportions to obtain a complex microorganism bacterium agent.
15 parts of bacillus subtilis, 15 parts of bacillus licheniformis, 15 parts of bacillus amyloliquefaciens, 15 parts of bacillus lateraporus, 5 parts of streptomyces griseus, 8 parts of thiobacillus denitrificans, 100 parts of starch and 50 parts of modified highland barley straw powder.
Example 4
Each dry powder of the microorganism in a dormant state was prepared in the same manner as in example 1, and the respective components were mixed in the following proportions to obtain a complex microorganism bacterium agent.
15 parts of bacillus subtilis, 15 parts of bacillus licheniformis, 15 parts of bacillus amyloliquefaciens, 15 parts of bacillus lateraporus, 8 parts of streptomyces griseus, 8 parts of thiobacillus denitrificans, 100 parts of starch and 50 parts of modified highland barley straw powder.
Example 5
Each dry powder of the microorganism in a dormant state was prepared in the same manner as in example 1, and the respective components were mixed in the following proportions to obtain a complex microorganism bacterium agent.
15 parts of bacillus subtilis, 15 parts of bacillus licheniformis, 15 parts of bacillus amyloliquefaciens, 15 parts of bacillus lateraporus, 17 parts of streptomyces griseus and 8 parts of thiobacillus denitrificans, 100 parts of starch and 50 parts of modified highland barley straw powder.
Example 6
Each dry powder of the microorganism in a dormant state was prepared in the same manner as in example 1, and the respective components were mixed in the following proportions to obtain a complex microorganism bacterium agent.
15 parts of bacillus subtilis, 15 parts of bacillus licheniformis, 15 parts of bacillus amyloliquefaciens, 15 parts of bacillus lateraporus, 20 parts of streptomyces griseus, 8 parts of thiobacillus denitrificans, 100 parts of starch and 50 parts of modified highland barley straw powder.
Example 7
Each dry powder of the microorganism in a dormant state was prepared in the same manner as in example 1, and the respective components were mixed in the following proportions to obtain a complex microorganism bacterium agent.
Preparing modified corn straw powder: taking dried and crushed corn straws sieved by a 100-mesh sieve, adding sodium bicarbonate with the mass of 10 times that of the straws and the concentration of 7 wt%, uniformly stirring, pretreating for 48 hours at 50 ℃, then washing the pretreated straws with deionized water for 3 times, and drying.
15 parts of bacillus subtilis, 15 parts of bacillus licheniformis, 15 parts of bacillus amyloliquefaciens, 15 parts of bacillus laterosporus, 13 parts of streptomyces griseus, 8 parts of thiobacillus denitrificans, 100 parts of starch and 50 parts of modified corn straw powder.
Example 8
Each dry powder of the microorganism in a dormant state was prepared in the same manner as in example 1, and the respective components were mixed in the following proportions to obtain a complex microorganism bacterium agent.
15 parts of bacillus subtilis, 15 parts of bacillus licheniformis, 15 parts of bacillus amyloliquefaciens, 15 parts of bacillus lateraporus, 13 parts of streptomyces griseus, 8 parts of thiobacillus denitrificans, 67 parts of starch and 33 parts of modified highland barley straw powder.
Example 9
The dry powder of each bacterium in a dormant state and the modified highland barley powder are prepared in the same manner as in example 1, and the components are mixed according to the following proportion to obtain the compound microbial agent.
15 parts of bacillus subtilis, 15 parts of bacillus licheniformis, 15 parts of bacillus amyloliquefaciens, 15 parts of bacillus lateraporus, 13 parts of streptomyces griseus, 8 parts of thiobacillus denitrificans, 120 parts of starch and 60 parts of modified highland barley straw powder.
Example 10
Each dry powder of the microorganism in a dormant state was prepared in the same manner as in example 1, and the respective components were mixed in the following proportions to obtain a complex microorganism bacterium agent.
15 parts of bacillus subtilis, 15 parts of bacillus licheniformis, 15 parts of bacillus amyloliquefaciens, 15 parts of bacillus lateraporus, 13 parts of streptomyces griseus, 8 parts of thiobacillus denitrificans, 3 parts of bacillus thuringiensis, 3 parts of bacillus cereus, 3 parts of bacillus polymyxa, 100 parts of starch and 50 parts of modified corn powder.
Example 11
Each dry powder of the microorganism in a dormant state was prepared in the same manner as in example 1, and each component was mixed in the following ratio to obtain a complex microorganism bacterium agent: 15 parts of bacillus subtilis, 15 parts of bacillus licheniformis, 15 parts of bacillus amyloliquefaciens, 15 parts of bacillus laterosporus, 13 parts of streptomyces griseus and 8 parts of thiobacillus denitrificans. Namely, the compound microbial inoculum corresponding to the example 1 is not added with a carbon source supplement.
Comparative example 1
The preparation and the component ratios were the same as in example 2, except that Streptomyces griseus was not included.
Comparative example 2
The preparation method and the component ratio were the same as those in example 2 except that Bacillus subtilis was not contained.
Comparative example 3
The preparation and ingredient ratios were the same as in example 2 except that no Bacillus licheniformis was included.
Application example
Preparation of nutrient solution
(1) A total of 30kg of seven to eight times cooked papaya, apple, mango and lemon from southern Hainan of origin were mixed in a ratio of 3: 15: 7: 5, respectively weighing, and washing for 2 times by using water;
(2) soaking the fruit in 40 wt% concentration sodium chloride solution for 30 min to sterilize, washing with deionized water for 3 times and cutting into fruit blocks of 5-10 cm length;
(3) juicing the fruit blocks with peel removed by a squeezer to obtain coarse pulp;
(4) placing the coarse pulp of each fruit in a fermentation tank with a cover, adding white sugar accounting for 8% of the weight of the coarse pulp into the fermentation tank, adding yeast powder accounting for 5% of the weight of the coarse pulp, fermenting in a sealed container at 35 ℃ for 25 days, opening a container cover every 1 day, ventilating, stirring uniformly, and fermenting in a sealed manner to obtain a fermentation stock solution;
(5) mixing the fermentation stock solutions of the fruits to obtain a mixed solution;
(6) placing the mixed solution in another sealed tank, adding 3 wt% of composite lactobacillus powder (Saishu Olympic Biotech Co., Ltd.) in the mixed solution, sealing and fermenting at 28 deg.C for 10 days, and filtering to obtain filtrate;
(7) the filtrate was mixed with deionized water at a ratio of 1: 20, and mixing to obtain the nutrient solution.
(III) Sewage treatment
1) Domestic sewage from municipal pipelines (raw water: COD 182mg/L, TN 48mg/L, TP 4.5mg/L, NH3-N ═ 32mg/L) are sequentially passed through a coarse grid well and a fine grid well to remove large-volume pollutants and impurities in water, wherein the meshes of the coarse grid are 20mm and the meshes of the fine grid are 3 mm;
2) the effluent of the fine grid well flows into a grit chamber to precipitate other impurities in the water;
3) the effluent of the grit chamber enters a mixing tank and is mixedThe total volume of the inlet water of the combined pool is 0.06g/m3D, adding the prepared nutrient solution in an amount which is 300 times that of the nutrient solution stock solution, and continuously adding the nutrient solution for 24 hours;
4) the effluent of the mixing tank enters an anaerobic tank and is fully mixed, wherein a three-dimensional biological rotating disc is arranged in the anaerobic tank, 50% of the area of the biological rotating disc is immersed in sewage, DO (DO) is 0.2mg/L, the biological rotating disc rotates at a constant speed of 5 revolutions per minute, and the hydraulic retention time is about 6 hours;
5) the effluent of the anaerobic tank flows into a biochemical tank, and the total volume of the effluent of the anaerobic tank is 100g/m3Adding the composite microbial inoculum of the embodiment and the comparative example, supplying air to the biochemical pool through an air blower, adjusting an air pipe valve to keep the dissolved oxygen amount in the biochemical pool at 2.5mg/L, setting a mixed liquid reflux pump of the effluent of the biochemical pool at the tail end of the biochemical pool, and refluxing to a mixed water distribution pool, wherein the reflux amount is 1Q;
6) effluent in the biochemical tank flows into a secondary sedimentation tank, sludge and water are separated in the secondary sedimentation tank, part of sludge in the secondary sedimentation tank flows back into the anaerobic tank through a sludge reflux pump, and the reflux quantity is 1Q;
7) and the effluent of the secondary sedimentation tank flows into the disinfection system and is discharged through the effluent metering tank.
Respectively treating the treated sewage with COD (chemical oxygen demand), TN, TP and NH3-N (ammonia nitrogen) is measured using industry or national standards. Wherein, the COD is measured by a potassium dichromate method (GBT 11914-89), the total N is measured by an alkaline potassium persulfate digestion ultraviolet spectrophotometry method (GB11894-89), the total P is measured by an ammonium molybdate spectrophotometry method (GB 11893-89), and the ammonia nitrogen is measured by a gas phase molecular absorption spectrometry method (HJ/T195-2005).
The measurement results are shown in table 1 below:
as can be seen from the comparison of example 1 and the comparative example in Table 1, the microbial inoculum in the absence of Streptomyces griseus, Bacillus subtilis, Bacillus licheniformis at TN, TP, NH3The removal rate of-N and COD is obviously lower than that of the compound microbial inoculum containing the N and the COD, which fully indicates that the streptomyces griseus, the bacillus subtilis and the bacillus licheniformis generate synergistic cooperation effect and are beneficial to removing excess N, P components and other pollutants in the sewage. In addition, the existence of the modified straw powder greatly promotes the growth and the propagation of dominant bacteria in the sewage, makes up the deficiency of the quick consumption of starch, and further improves the excessive N, P in the sewage and the decomposition and the removal of organic pollutants. In addition, the long-term efficacy of the complex microbial inoculant was also tested, and the discharged water was measured on the 7 th day of addition of the complex microbial inoculant of example 1 without additional supplementation of the complex microbial inoculant, and as a result, it was found that: the TN removal rate of the compound microbial agent is still more than 76%, the TP removal rate is more than 89%, and NH is generated3The N removal rate is more than 93 percent, and the COD removal rate is more than 90 percent; on the other hand, the complex microbial agent of example 11 was added, and the effluent was measured on day 7, and as a result, it was found that: TN removal rate is about 70%, TP removal rate is about 82%, and NH3The removal rate of-N is 86 percent, the removal rate of COD is about 85 percent, which shows that various bacteria in the compound microbial agent can maintain the advantages of the bacteria and have good activity for a long time, and under the action of cellulase, hemicellulase and the like secreted by microorganisms, starch and straw treated by weak alkali finally generate glucose to be used as a supplementary carbon source of various microorganisms, thereby maintaining the normal physiological metabolism of the microorganisms for sewage treatment.
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Various alternatives, modifications and combinations of the features of the invention can be made without departing from the spirit and nature of the invention as claimed, and such simple variations and combinations should also be considered as disclosed in the present application, all falling within the scope of the invention.
Claims (10)
1. The compound microbial agent for sewage treatment is characterized by being prepared from the following raw materials: bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus laterosporus, Streptomyces griseus and Thiobacillus denitrificans.
2. The compound microbial agent according to claim 1, which is prepared from the following raw materials in parts by weight: 10-20 parts of bacillus subtilis, 10-20 parts of bacillus licheniformis, 10-20 parts of bacillus amyloliquefaciens, 10-20 parts of bacillus laterosporous, 5-20 parts of streptomyces griseus and 5-10 parts of thiobacillus denitrificans.
3. The complex microbial inoculant according to claim 2, wherein the raw materials of the complex microbial inoculant further comprise 100-200 parts of a carbon source supplement; preferably, the carbon source supplement is starch and/or weak base modified straw powder, wherein the weak base is selected from one or more of sodium carbonate, potassium carbonate, sodium bicarbonate, potassium bicarbonate, ammonia water and urea; more preferably, the carbon source supplement is prepared by mixing starch and weak base modified straw powder according to a mass ratio of 1-2: 1-2.
4. The compound microbial inoculant according to claim 3, wherein the straw is selected from one or more of highland barley, corn, wheat, rice and cotton.
5. The complex microbial inoculant according to claim 1, wherein the raw materials of the complex microbial inoculant further comprise: one or more of bacillus thuringiensis, bacillus cereus and bacillus polymyxa.
6. The complex microbial inoculant according to claim 5, wherein the raw materials of the complex microbial inoculant further comprise: 1-5 parts of bacillus thuringiensis, 1-5 parts of bacillus cereus and 1-5 parts of bacillus polymyxa.
7. The compound microbial inoculant according to claim 3, wherein the preparation method of the weak base modified straw powder comprises the following steps: taking the dried and crushed straw sieved by a sieve of 80-100 meshes, adding a weak base solution with the concentration of 10-20 wt%, uniformly mixing, pretreating for 48-72 hours at 40-55 ℃, then washing the pretreated straw with deionized water for 3-5 times, and drying.
8. The method for preparing the complex microbial agent of any one of claims 1 to 7, comprising the steps of:
1) respectively inoculating slant strains of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus laterospora, Streptomyces griseus and Thiobacillus denitrificans into respective appropriate liquid culture medium, and performing liquid fermentation culture until the viable bacteria concentration of each bacterium in the liquid fermentation liquid is not less than 1 × 109CFU/ml, dehydrating and drying to obtain dormant microorganism dry powder;
2) mixing the prepared dormant microorganism dry powder according to a preset proportion to obtain a compound microorganism bacterium agent;
optionally, in the step 1), at least one of bacillus thuringiensis, bacillus cereus and bacillus polymyxa is also added for liquid fermentation culture.
9. The method of claim 8, wherein in step 2), a carbon source supplement is added.
10. Use of the complex microbial inoculant according to any one of claims 1 to 7 or prepared according to the preparation method of claim 8 or 9 in the treatment of sewage, especially eutrophicated sewage.
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