CN104830740A - Preparation and application methods of efficient microorganism agent for treating compound fertilizer wastewater - Google Patents

Preparation and application methods of efficient microorganism agent for treating compound fertilizer wastewater Download PDF

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CN104830740A
CN104830740A CN201510283409.3A CN201510283409A CN104830740A CN 104830740 A CN104830740 A CN 104830740A CN 201510283409 A CN201510283409 A CN 201510283409A CN 104830740 A CN104830740 A CN 104830740A
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bacteria
sewage
mixed
composite fertilizer
glucose
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龚国利
张甜
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Shaanxi University of Science and Technology
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Shaanxi University of Science and Technology
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Abstract

The invention discloses preparation and application methods of an efficient microorganism agent for treating compound fertilizer wastewater. Nitrate bacteria, nitrite bacteria and phosphorus-accumulating bacteria are used to convert ammonia nitrogen in the wastewater into nitrogen and phosphorus into polyphosphate accumulating in the bacteria; zeolite, Ca(OH)2 and anhydrous sodium sulfite are used to settle ions, such as fluorine and cyanogen, and the dirty matters can be effectively removed; glucose can be added to keep certain nitrification rate; a triangular flask is used for enlarged cultivation, the highest growth needs of various bacteria can be met, enlarged mixed cultivation of a fermenting tank helps save resources, each bacterium has satisfied concentration, cultivating is easy, and the water treated meets the national emission standard. The efficient microorganism agent has good effect of decontaminating the compound fertilizer wastewater, microorganisms may vanish with reduction in pollutants, environmental damage is avoided, and the efficient microorganism agent has low cost and promising application prospect.

Description

The high-effective microorganism bacterial preparation process of process composite fertilizer sewage and using method
Technical field
The present invention relates to technical field of sewage, particularly relate to a kind of the high-effective microorganism bacterial preparation process and the using method that process composite fertilizer's sewage.
Background technology
Three elements (nitrogen containing two kinds of fertilizer in fertilizer, phosphorus, potassium) binary compound, mixed fertilizer and containing nitrogen, phosphorus, the tri compound of potassium three kinds of elements, mixed fertilizer is referred to as composite fertilizer, owing to being rich in various nutrient elements, therefore promote throughout the country rapidly, composite fertilizer of consequent process fertilizer plant sewage becomes a problem, wherein, the principal pollutant of ammonia nitrogen and factory of Lin Shi composite fertilizer, enter water body and can cause body eutrophication, cause water quality severe, ammonia nitrogen is mainly from synthetic ammonia, this part ammonia nitrogen of the high-concentration ammonia nitrogenous wastewater of urea plant mainly exists with inorganic nitrogen, phosphorus is mainly from Rock Phosphate (72Min BPL), increasing of Inorganic phosphate can make Measures of Algae in Water Body ramp, destroy water quality, the main source of fertilizer plant's pollutent is paper waste, the desulfurization wastewater of desulfurization process generation and the ammonia-containing water of copper solution scrubbing process generation that gasification process produces, the feature of composite fertilizer is then flow into ammonia nitrogen too much in waste water, phosphorus etc. aborning can cause body eutrophication, also have fluorine, cyanogen plasma to flow out, these ions have toxicity simultaneously.
Fluorine in tap water is comparatively large on the impact of HUMAN HEALTH, and the fluorine of long-term excess intake can make body produce various fluorosis symptom, common are nausea and vomiting, salivation, itch, diarrhoea and other intestinal tract diseases, and nature has certain harm to hydrobiont; Most inorganic cyanide all belongs to high poison, highly toxic substance, the prussiate of minute quantity will make people and animals be poisoned to death in the section time, below standard fluorine-containing, cyanogen discharge of wastewater enter in physical environment, enter in surface water and groundwater by the mode such as rainwash, infiltration, the ecosystem is destroyed, and even serious threat is to the existence of aquatic animals and plants; Waste disposal plant conventional at present and treatment process, clean effect is not too desirable, and particularly factory of composite fertilizer sewage, because sewage situation is complicated, clean effect is poorer, and cost is high.
Summary of the invention
The present invention is directed to factory of composite fertilizer sewage feature, propose a kind of the high-effective microorganism bacterial preparation process and the using method that process composite fertilizer's sewage, microbiobacterial agent of the present invention has extraordinary clean effect to composite fertilizer's sewage, and microbes fades away along with the minimizing of pollutent, also can not work the mischief to environment, complex micro organism fungicide cost of the present invention is low, is with a wide range of applications.
In order to achieve the above object, the technical solution used in the present invention is: the high-effective microorganism bacterial preparation process 1, processing composite fertilizer's sewage, is characterized in that, comprise the following steps:
The activation of steps A, each bacterial classification:
Nitrobacteria, nitrite bacteria and polyP bacteria are activated on respective solid medium;
Step B, triangular flask enlarged culturing:
Preparing each strain liquid substratum respectively loads in 250mL triangular flask, and load liquid nutrient medium 100mL, entered by the colony inoculation that solid medium grows in independent triangular flask, shaking table is cultivated, and cultivates thalline quantity and reaches 1.0 × 10 8-6.0 × 10 8individual/mL; Step C, fermentor tank mixing enlarged culturing:
Mixed culture medium 3.5-4L is added in fermentor tank, inoculates nitrobacteria, nitrite bacteria and polyP bacteria respectively and enter in fermentor tank to cultivate, cultivate mixed strains thalline quantity and reach 1.0 × 10 8-2.0 × 10 8individual/mL;
Step D, centrifugal, collecting precipitation, drying obtain mixed bacterium powder:
By the mixed strains nutrient solution that obtains in step C through centrifugal collecting precipitation, then drying will be precipitated, obtain mixed bacterium powder; The preparation of step e, complex micro organism fungicide:
The mixed bacterium powder that step D is obtained and zeolite, Ca (OH) 2, sodium sulphite anhydrous 99.3, glucose, copper sulfate is mixed and made into complex micro organism fungicide.
In step e, in mass fraction, wherein mixed bacterium powder 50-60 part, zeolite 30-50 part, Ca (OH) 21-2 part, sodium sulphite anhydrous 99.3 0.5-1 part, glucose 0.5-1 part and copper sulfate 0.01-0.02 part; Wherein zeolite is pulverized, and crosses 80-120 mesh sieve.
Described nitrobacteria is one or both of Pseudomonas aeruginosa (Pseudomonas aeruginosa) CGMCC No.150 and ammonia oxidation Selective medium (Pseudonocardia ammonioxydans) CGMCC No.41877;
Described nitrite bacteria is ammonia oxidizing bacteria (Ammonia oxidizing bacteria) CGMCC No.4287;
Described polyP bacteria is one or more in enteroaerogen (Enterbacter aerogenes) CGMCC No.16753, Paracoccus denitrificans (Paracoccus denitrificans) CGMCC No.15358, subtilis (Bacillus subtillus) CGMCC No.17408 and iron protoxide Thiobacillus (Acidithiobacillus ferrooxidans) CGMCC No.15066.
Above-mentioned bacterial classification all can be bought from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) and obtain.
Described ammonia oxidation false promise Ka Shi bacterial liquid nutrient media components is: glucose 10g, yeast leaching powder 10g, distilled water 1L;
Described ammonia oxidizing bacteria liquid nutrient medium component is: (NH 4) 2sO 40.5g, NaCl 2g, FeSO 47H 2o 0.4g, K 2hPO 41g, MgSO 47H 2o 0.5g, NaCO 35g, distilled water 1L, adjust ph is 7.2;
Described subtilis liquid nutrient medium component is: glucose 5g, peptone 5g, NaCl 3g, extractum carnis 0.5g, distilled water 1L;
Described enteroaerogen liquid nutrient medium component is: (NH 4) 2sO 41g, NaCl 0.1g, glucose 5g, Na 2hPO 42g, MgSO 47H 2o 0.5g, yeast leaching powder 0.5g, NaCO 31g, distilled water 1L, adjust ph is 7.0;
The solid medium of above-mentioned each bacterial classification for add 15-20g agar powder respectively in its liquid nutrient medium, and at 121 DEG C of sterilizing 30min.
Described mixed culture medium component is: glucose 10g, yeast leaching powder 10g, NaCl1g, (NH 4) 2sO 41g, K 2hPO 41g, MgSO 47H 2o0.5g, FeSO 47H 2o3g, NaCO 32g, distilled water 1L, adjust ph is 7.0.
In step B, the bacterium colony in access triangular flask is single bacterium colony, and shaking speed is 150r/min.
In step C, inoculating nitrobacteria, nitrite bacteria and polyP bacteria amount is respectively the 5-10% of fermenter volume.
In step D, centrifugation rate is 4000r/min, and drying temperature is 30-40 DEG C.
In steps A, step B and step C, incubation time is 72-96h, and culture temperature is 28-30 DEG C.
The high-effective microorganism microbial inoculum using method of process composite fertilizer sewage, first, with Ca (OH) 2sewage pH is regulated to be 7.0-8.0, then, at every 20m 3drop into this complex micro organism fungicide 0.5-1Kg in sewage, when disposing of sewage, slowly in sewage, pass into air.
Compared with prior art, the present invention at least has following beneficial effect: the present invention utilizes triangular flask enlarged culturing respectively, the most Seedling height needs of each bacterial classification can be reached, fermentor tank enlarged culturing economizes on resources, and each bacterial classification reaches requirement concentration, and easily cultivates, glucose in complex micro organism fungicide can promote the growth of bacterial classification, can maintain certain digestion rate, this complex micro organism fungicide is with low cost, complex micro organism fungicide mesolite, Ca (OH) 2, sodium sulphite anhydrous 99.3, copper sulfate etc. fluorine, the cyanogen plasma that can effectively remove in sewage, complex microorganism can reduce ammonia nitrogen and phosphorus etc. rapidly, reduce body eutrophication, the water quality after process is made to reach discharging standards, be with a wide range of applications, the clearance of the present invention to ammonia nitrogen in sewage reaches 98%, and the clearance of phosphorus is 96.5%, the clearance of fluorine reaches 99%, and the clearance of cyanogen reaches 97.9%.
Complex microorganism of the present invention is not containing pathogenic agent and pathogenic bacterium, the organism in sewage is utilized to meet idiotrophic demand, can effective degradation of sewage pollutent, after sewage is purified, these microbes fade away along with the minimizing of pollutent, also can not work the mischief to environment.
Embodiment
Below in conjunction with specific embodiment, the present invention is described further, described in be explanation of the invention instead of restriction, any enlarged culturing makes cell concentration meet the requirements of training method.
The nitrobacteria that the present invention relates to is one or both of Pseudomonas aeruginosa (Pseudomonas aeruginosa) CGMCC No.150 and ammonia oxidation Selective medium (Pseudonocardia ammonioxydans) CGMCC No.41877;
Nitrite bacteria is ammonia oxidizing bacteria (Ammonia oxidizing bacteria) CGMCC No.4287;
PolyP bacteria is one or more in enteroaerogen (Enterbacter aerogenes) CGMCC No.16753, Paracoccus denitrificans (Paracoccusdenitrificans) CGMCC No.15358, subtilis (Bacillus subtillus) CGMCC No.17408 and iron protoxide Thiobacillus (Acidithiobacillus ferrooxidans) CGMCC No.15066.
Above-mentioned bacterial classification all can be bought from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) and obtain.
In embodiments of the invention, the liquid nutrient medium component of each bacterial classification is as follows:
Ammonia oxidation false promise Ka Shi bacteria culture medium: glucose 10g, yeast leaching powder 10g, distilled water 1L;
Ammonia oxidizing bacteria substratum: (NH 4) 2sO 40.5g, NaCl 2g, FeSO 47H 2o 0.4g, K 2hPO 41g, MgSO 47H 2o0.5g, NaCO 35g, distilled water 1L, adjust ph is 7.2;
Bacillus subtilis bacterium culture medium: glucose 5g, peptone 5g, NaCl 3g, extractum carnis 0.5g, distilled water 1L;
Enteroaerogen substratum (NH 4) 2sO 41g, NaCl 0.1g, glucose 5g, Na 2hPO 42g, MgSO 47H 2o 0.5g, yeast leaching powder 0.5g, NaCO 31g, distilled water 1L, adjust ph is 7.0;
The solid medium of above-mentioned each bacterial classification for add 15-20g agar powder respectively in its liquid nutrient medium, and at 121 DEG C of sterilizing 30min.
Mixed culture medium component used in the present invention is: glucose 10g, yeast leaching powder 10g, NaCl1g, (NH 4) 2sO 41g, K 2hPO 41g, MgSO 47H 2o0.5g, FeSO 47H 2o3g, NaCO 32g, distilled water 1L, adjust ph is 7.0.
Embodiment 1
The activation of each bacterial classification of steps A
Employing different culture media is cultivated, by the strain inoculation of ammonia oxidation Selective medium, ammonia oxidizing bacteria and enteroaerogen on solid medium 28-30 DEG C cultivate 72-96h.
Step B triangular flask enlarged culturing
In preparation process A, the liquid nutrient medium of each bacterial classification loads in 250mL triangular flask respectively, load liquid nutrient medium 100mL, enter in independent triangular flask by single colony inoculation of the suitable size that solid medium grows, shaking table 150r/min, 30 DEG C of cultivation 96h make thalline content reach 1.0 × 10 8-6.0 × 10 8individual/mL;
Step C fermentor tank mixing enlarged culturing
Add in fermentor tank by mixed culture medium 4L, inoculating ammonia oxidation Selective medium, ammonia oxidizing bacteria and enteroaerogen is respectively that 5%, 8%, 10%, 30 DEG C of mixed culture medium volume are cultivated 72h and made mixed strains thalline content reach 1.0 × 10 8-2.0 × 10 8individual/mL;
Step D complex microorganism, collecting precipitation, drying obtain mixed bacterium powder
By the mixed strains nutrient solution that obtains in step C through 4000r/min centrifugal collecting precipitation, then 30 DEG C of dryings will be deposited in, obtain mixed bacterium powder;
The preparation of step e complex micro organism fungicide
The mixed bacterium powder that step D is obtained and zeolite, Ca (OH) 2, sodium sulphite anhydrous 99.3, glucose is mixed and made into complex micro organism fungicide, in mass fraction, wherein 50 parts, mixed bacterium powder, 40 parts, zeolite, Ca (OH) 21 part, sodium sulphite anhydrous 99.3 0.5 part, glucose 0.5-1 part, copper sulfate 0.01-0.02 part; Wherein zeolite is pulverized, and crosses 80-120 mesh sieve.
The use of step F complex micro organism fungicide
First, with Ca (OH) 2sewage pH is regulated to be 7.0-8.0, then to every 20m 3drop into this complex micro organism fungicide 0.5-1Kg in sewage, slowly in sewage, pass into air.
Embodiment 2
The activation of each bacterial classification of steps A
Employing different culture media is cultivated, by the strain inoculation of Pseudomonas aeruginosa, ammonia oxidizing bacteria and Paracoccus denitrificans on solid medium 28-30 DEG C cultivate 72-96h.
Step B triangular flask enlarged culturing
In preparation process A, the liquid nutrient medium of each bacterial classification loads in 250mL triangular flask respectively, load liquid nutrient medium 100mL, enter in independent triangular flask by single colony inoculation of the suitable size that solid medium grows, shaking table 150r/min, 30 DEG C of cultivation 72-96h make thalline content reach 1.0 × 10 8-2.0 × 10 8individual/mL;
Step C fermentor tank mixing enlarged culturing
Mixed culture medium 4L is added in fermentor tank, inoculate that Pseudomonas aeruginosa, ammonia oxidizing bacteria and Paracoccus denitrificans are mixed culture medium volume respectively 10%, 10%, 5%, cultivate 72h for 30 DEG C and make mixed strains thalline content reach 1.0 × 10 8-2.0 × 10 8individual/mL;
Step D complex microorganism, collecting precipitation, drying obtain mixed bacterium powder
By the mixed strains nutrient solution that obtains in step C through 4000r/min centrifugal collecting precipitation, then 30 DEG C of dryings will be deposited in, obtain mixed bacterium powder;
The preparation of E complex micro organism fungicide
The mixed bacterium powder that step D is obtained and zeolite, Ca (OH) 2, sodium sulphite anhydrous 99.3, glucose is mixed and made into complex micro organism fungicide, in mass, wherein 55 parts, mixed bacterium powder, 40 parts, zeolite, Ca (OH) 21.5 parts, sodium sulphite anhydrous 99.3 0.5 part, glucose 0.5 part, 0.02 part, copper sulfate.Wherein zeolite is pulverized, and crosses 80-120 mesh sieve.
The use of F complex micro organism fungicide
First, with Ca (OH) 2sewage pH is regulated to be 7.0-8.0, then to every 20m 3drop into this complex micro organism fungicide 0.5-1Kg in sewage, slowly in sewage, pass into air.
Embodiment 3
The activation of each bacterial classification of steps A
Employing different culture media is cultivated, by the strain inoculation of Pseudomonas aeruginosa, ammonia oxidation Selective medium, ammonia oxidizing bacteria and subtilis on solid medium 28-30 DEG C cultivate 72-96h.
Step B triangular flask enlarged culturing
In preparation process A, the liquid nutrient medium of each bacterial classification loads in 250mL triangular flask respectively, load liquid nutrient medium 100mL, enter in independent triangular flask by single colony inoculation of the suitable size that solid medium grows, shaking table 150r/min, 30 DEG C of cultivation 96h make thalline content reach 5.0 × 10 8-6.0 × 10 8individual/mL;
Step C fermentor tank mixing enlarged culturing
Mixed culture medium 4L is added in fermentor tank, inoculate that Pseudomonas aeruginosa, ammonia oxidation Selective medium, ammonia oxidizing bacteria and subtilis are mixed culture medium volume respectively 3%, 5%, 5%, cultivating 72h for 8%, 30 DEG C makes mixed strains thalline content reach 1.0 × 10 8-2.0 × 10 8individual/mL;
Step D complex microorganism, collecting precipitation, drying obtain mixed bacterium powder
By the mixed strains nutrient solution that obtains in step C through 4000r/min centrifugal collecting precipitation, then 30 DEG C of dryings will be deposited in, obtain mixed bacterium powder;
The preparation of step e complex micro organism fungicide
The mixed bacterium powder that step D is obtained and zeolite, Ca (OH) 2, sodium sulphite anhydrous 99.3, glucose is mixed and made into complex micro organism fungicide, in mass, wherein 55 parts, mixed bacterium powder, 35 parts, zeolite, Ca (OH) 21 part, sodium sulphite anhydrous 99.3 1 part, glucose 0.5 part, 0.02 part, copper sulfate.Wherein zeolite is pulverized, and crosses 80-120 mesh sieve.
The use of step F complex micro organism fungicide
First, with Ca (OH) 2sewage pH is regulated to be 7.0-8.0, then to every 20m 3drop into this complex micro organism fungicide 0.5-1Kg in sewage, slowly in sewage, pass into air.
Embodiment 4
The activation of each bacterial classification of steps A
Employing different culture media is cultivated, by the strain inoculation of Pseudomonas aeruginosa, ammonia oxidation Selective medium, ammonia oxidizing bacteria and iron protoxide Thiobacillus on solid medium 28-30 DEG C cultivate 96h.
Step B triangular flask enlarged culturing
In preparation process A, the liquid nutrient medium of each bacterial classification loads in 250mL triangular flask respectively, load liquid nutrient medium 100mL, enter in independent triangular flask by single colony inoculation of the suitable size that solid medium grows, shaking table 150r/min, 30 DEG C of cultivation 96h make thalline content reach 3.0 × 10 8-4.0 × 10 8individual/mL;
Step C fermentor tank mixing enlarged culturing
Mixed culture medium 4L is added in fermentor tank, inoculate that Pseudomonas aeruginosa, ammonia oxidation Selective medium, ammonia oxidizing bacteria and iron protoxide Thiobacillus are mixed culture medium volume respectively 6%, 2%, 6%, cultivating 72h for 5%, 30 DEG C makes mixed strains thalline content reach 1.0 × 10 8-2.0 × 10 8individual/mL;
Step D complex microorganism, collecting precipitation, drying obtain mixed bacterium powder
By the mixed strains nutrient solution that obtains in step C through 4000r/min centrifugal collecting precipitation, then 30 DEG C of dryings will be deposited in, obtain mixed bacterium powder;
The preparation of step e complex micro organism fungicide
The mixed bacterium powder that step D is obtained and zeolite, Ca (OH) 2, sodium sulphite anhydrous 99.3, glucose is mixed and made into complex micro organism fungicide, in mass, wherein 50 parts, mixed bacterium powder, 45 parts, zeolite, Ca (OH) 21 part, sodium sulphite anhydrous 99.3 1 part, glucose 1 part, 0.01 part, copper sulfate.Wherein zeolite is pulverized, and crosses 80-120 mesh sieve.
The use of step F complex micro organism fungicide
First, with Ca (OH) 2sewage pH is regulated to be 7.0-8.0, then to every 20m 3drop into this complex micro organism fungicide 0.5-1Kg in sewage, slowly in sewage, pass into air.
Embodiment 5
The activation of each bacterial classification of steps A
Employing different culture media is cultivated, by the strain inoculation of Pseudomonas aeruginosa, ammonia oxidizing bacteria, Paracoccus denitrificans and subtilis on solid medium 28-30 DEG C cultivate 72h.
Step B triangular flask enlarged culturing
In preparation process A, the liquid nutrient medium of each bacterial classification loads in 250mL triangular flask respectively, load liquid nutrient medium 100mL, enter in independent triangular flask by single colony inoculation of the suitable size that solid medium grows, shaking table 150r/min, 30 DEG C of cultivation 96h make thalline content reach 1.0 × 10 8-6.0 × 10 8individual/mL;
Step C fermentor tank mixing enlarged culturing
Mixed culture medium 4L is added in fermentor tank, inoculate that Pseudomonas aeruginosa, ammonia oxidizing bacteria, Paracoccus denitrificans and subtilis are mixed culture medium volume respectively 5%, 7%, 5%, cultivate 72h for 5%, 30 DEG C and make mixed strains thalline content reach 1.0 × 10 8-2.0 × 10 8individual/mL;
Step D complex microorganism, collecting precipitation, drying obtain mixed bacterium powder
By the mixed strains nutrient solution that obtains in step C through 4000r/min centrifugal collecting precipitation, then 30 DEG C of dryings will be deposited in, obtain mixed bacterium powder;
The preparation of step e complex micro organism fungicide
The mixed bacterium powder that step D is obtained and zeolite, Ca (OH) 2, sodium sulphite anhydrous 99.3, glucose is mixed and made into complex micro organism fungicide, in mass, wherein 60 parts, mixed bacterium powder, 35 parts, zeolite, Ca (OH) 22 parts, sodium sulphite anhydrous 99.3 1 part, glucose 1 part, 0.01 part, copper sulfate.Wherein zeolite is pulverized, and crosses 80-120 mesh sieve.
The use of step F complex micro organism fungicide
First, with Ca (OH) 2sewage pH is regulated to be 7.0-8.0, then to every 20m 3drop into this complex micro organism fungicide 0.5-1Kg in sewage, slowly in sewage, pass into air.
Embodiment 6
The activation of each bacterial classification of steps A
Employing different culture media is cultivated, by the strain inoculation of ammonia oxidation Selective medium, ammonia oxidizing bacteria, enteroaerogen, Paracoccus denitrificans, subtilis and iron protoxide Thiobacillus on solid medium 28-30 DEG C cultivate 72-96h.
Step B triangular flask enlarged culturing
In preparation process A, the liquid nutrient medium of each bacterial classification loads in 250mL triangular flask respectively, load liquid nutrient medium 100mL, enter in independent triangular flask by single colony inoculation of the suitable size that solid medium grows, shaking table 150r/min, 30 DEG C of cultivation 96h make thalline content reach 1.0 × 10 8-6.0 × 10 8individual/mL;
Step C fermentor tank mixing enlarged culturing
Mixed culture medium 4L is added in fermentor tank, inoculating ammonia oxidation Selective medium, ammonia oxidizing bacteria, enteroaerogen, Paracoccus denitrificans, subtilis and iron protoxide Thiobacillus is respectively 10% of mixed culture medium volume, 9%, 2%, 3%, 2%, 2%, cultivate 72h for 30 DEG C and make mixed strains thalline content reach 1.0 × 10 8-2.0 × 10 8individual/mL;
Step D complex microorganism, collecting precipitation, drying obtain mixed bacterium powder
By the mixed strains nutrient solution that obtains in step C through 4000r/min centrifugal collecting precipitation, then 30 DEG C of dryings will be deposited in, obtain mixed bacterium powder;
The preparation of step e complex micro organism fungicide
The mixed bacterium powder that step D is obtained and zeolite, Ca (OH) 2, sodium sulphite anhydrous 99.3, glucose is mixed and made into complex micro organism fungicide, in mass, wherein 60 parts, mixed bacterium powder, 30 parts, zeolite, Ca (OH) 21.5 parts, sodium sulphite anhydrous 99.3 0.5 part, glucose 1 part, 0.01 part, copper sulfate.Wherein zeolite is pulverized, and crosses 80-120 mesh sieve.
The use of step F complex micro organism fungicide
First, with Ca (OH) 2sewage pH is regulated to be 7.0-8.0, then to every 20m 3drop into this complex micro organism fungicide 0.5-1Kg in sewage, slowly in sewage, pass into air.
Embodiment 7
The activation of each bacterial classification of steps A
Employing different culture media is cultivated, by the strain inoculation of Pseudomonas aeruginosa, ammonia oxidation Selective medium, ammonia oxidizing bacteria, enteroaerogen, subtilis and iron protoxide Thiobacillus on solid medium 28-30 DEG C cultivate 84h.
Step B triangular flask enlarged culturing
In preparation process A, the liquid nutrient medium of each bacterial classification loads in 250mL triangular flask respectively, load liquid nutrient medium 100mL, enter in independent triangular flask by single colony inoculation of the suitable size that solid medium grows, shaking table 150r/min, 30 DEG C of cultivation 96h make thalline content reach 1.0 × 10 8-6.0 × 10 8individual/mL;
Step C fermentor tank mixing enlarged culturing
Mixed culture medium 4L is added in fermentor tank, inoculating Pseudomonas aeruginosa, ammonia oxidation Selective medium, ammonia oxidizing bacteria, enteroaerogen, subtilis and iron protoxide Thiobacillus is respectively 6% of mixed culture medium volume, 1%, 10%, 3%, 3%, 1%, cultivate 72h for 30 DEG C and make mixed strains thalline content reach 1.0 × 10 8-2.0 × 10 8individual/mL;
Step D complex microorganism, collecting precipitation, drying obtain mixed bacterium powder
By the mixed strains nutrient solution that obtains in step C through 4000r/min centrifugal collecting precipitation, then 30 DEG C of dryings will be deposited in, obtain mixed bacterium powder;
The preparation of step e complex micro organism fungicide
The mixed bacterium powder that step D is obtained and zeolite, Ca (OH) 2, sodium sulphite anhydrous 99.3, glucose is mixed and made into complex micro organism fungicide, in mass, wherein 55 parts, mixed bacterium powder, 40 parts, zeolite, Ca (OH) 21 part, sodium sulphite anhydrous 99.3 0.5 part, glucose 0.5 part, 0.01 part, copper sulfate.Wherein zeolite is pulverized, and crosses 80-120 mesh sieve.
The use of step F complex micro organism fungicide
First, with Ca (OH) 2sewage pH is regulated to be 7.0-8.0, then to every 20m 3drop into this complex micro organism fungicide 0.5-1Kg in sewage, slowly in sewage, pass into air.
Complex microorganism of the present invention is not containing pathogenic agent and pathogenic bacterium, the organism in sewage is utilized to meet idiotrophic demand, can effective degradation of sewage pollutent, after sewage is purified, these microbes fade away along with the minimizing of pollutent, also can not work the mischief to environment, reach 98% to the clearance of ammonia nitrogen in sewage, the clearance of phosphorus is 96.5%, and the clearance of fluorine reaches 99%, the clearance of cyanogen reaches 97.9%, reaches emission standard.

Claims (10)

1. process the high-effective microorganism bacterial preparation process of composite fertilizer's sewage, it is characterized in that, comprise the following steps:
The activation of steps A, each bacterial classification:
Nitrobacteria, nitrite bacteria and polyP bacteria are activated on solid medium;
Step B, triangular flask enlarged culturing:
Preparing each strain liquid substratum respectively loads in 250mL triangular flask, and load liquid nutrient medium 100mL, entered by the colony inoculation that solid medium grows in independent triangular flask, shaking table is cultivated, and cultivates thalline quantity and reaches 1.0 × 10 8-6.0 × 10 8individual/mL; Step C, fermentor tank mixing enlarged culturing:
Mixed culture medium 3.5-4L is added in fermentor tank, inoculates nitrobacteria, nitrite bacteria and polyP bacteria respectively and enter in fermentor tank to cultivate, cultivate mixed strains thalline quantity and reach 1.0 × 10 8-2.0 × 10 8individual/mL;
Step D, centrifugal, collecting precipitation, drying obtain mixed bacterium powder:
By the mixed strains nutrient solution that obtains in step C through centrifugal collecting precipitation, then drying will be precipitated, obtain mixed bacterium powder; The preparation of step e, complex micro organism fungicide:
The mixed bacterium powder that step D is obtained and zeolite, Ca (OH) 2, sodium sulphite anhydrous 99.3, glucose, copper sulfate is mixed and made into complex micro organism fungicide.
2. the high-effective microorganism bacterial preparation process of the process composite fertilizer sewage according to claims 1, is characterized in that, in step e, in mass fraction, and wherein mixed bacterium powder 50-60 part, zeolite 30-50 part, Ca (OH) 21-2 part, sodium sulphite anhydrous 99.3 0.5-1 part, glucose 0.5-1 part and copper sulfate 0.01-0.02 part; Wherein zeolite is pulverized, and crosses 80-120 mesh sieve.
3. the high-effective microorganism bacterial preparation process of the process composite fertilizer sewage according to claims 1, it is characterized in that, described nitrobacteria is one or both of Pseudomonas aeruginosa (Pseudomonas aeruginosa) CGMCC No.150 and ammonia oxidation Selective medium (Pseudonocardia ammonioxydans) CGMCC No.41877;
Described nitrite bacteria is ammonia oxidizing bacteria (Ammonia oxidizing bacteria) CGMCC No.4287;
Described polyP bacteria is one or more in enteroaerogen (Enterbacter aerogenes) CGMCC No.16753, Paracoccus denitrificans (Paracoccus denitrificans) CGMCC No.15358, subtilis (Bacillus subtillus) CGMCC No.17408 and iron protoxide Thiobacillus (Acidithiobacillus ferrooxidans) CGMCC No.15066.
4. the high-effective microorganism bacterial preparation process of the process composite fertilizer sewage according to claims 3, is characterized in that,
Described ammonia oxidation false promise Ka Shi bacterial liquid nutrient media components is: glucose 10g, yeast leaching powder 10g, distilled water 1L;
Described ammonia oxidizing bacteria liquid nutrient medium component is: (NH 4) 2sO 40.5g, NaCl 2g, FeSO 47H 2o 0.4g, K 2hPO 41g, MgSO 47H 2o 0.5g, NaCO 35g, distilled water 1L, adjust ph is 7.2;
Described subtilis liquid nutrient medium component is: glucose 5g, peptone 5g, NaCl 3g, extractum carnis 0.5g, distilled water 1L;
Described enteroaerogen liquid nutrient medium component is: (NH 4) 2sO 41g, NaCl 0.1g, glucose 5g, Na 2hPO 42g, MgSO 47H 2o 0.5g, yeast leaching powder 0.5g, NaCO 31g, distilled water 1L, adjust ph is 7.0;
The solid medium of above-mentioned each bacterial classification for add 15-20g agar powder respectively in its liquid nutrient medium, and at 121 DEG C of sterilizing 30min.
5. the high-effective microorganism bacterial preparation process of the process composite fertilizer sewage according to claims 3, is characterized in that, described mixed culture medium component is: glucose 10g, yeast leaching powder 10g, NaCl 1g, (NH 4) 2sO 41g, K 2hPO 41g, MgSO 47H 2o 0.5g, FeSO 47H 2o 3g, NaCO 32g, distilled water 1L, adjust ph is 7.0.
6. the high-effective microorganism bacterial preparation process of the process composite fertilizer sewage according to claims 1, is characterized in that, in step B, the bacterium colony in access triangular flask is single bacterium colony, and shaking speed is 150r/min.
7. the high-effective microorganism bacterial preparation process of the process composite fertilizer sewage according to claims 1, is characterized in that, in step C, inoculating nitrobacteria, nitrite bacteria and polyP bacteria amount is respectively the 5-10% of fermenter volume.
8. the high-effective microorganism bacterial preparation process of the process composite fertilizer sewage according to claims 1, is characterized in that, in step D, centrifugation rate is 4000r/min, and drying temperature is 30-40 DEG C.
9. the high-effective microorganism bacterial preparation process of the process composite fertilizer sewage according to claims 1, is characterized in that, in steps A, step B and step C, incubation time is 72-96h, and culture temperature is 28-30 DEG C.
10. process the high-effective microorganism microbial inoculum using method of composite fertilizer's sewage, it is characterized in that, first, with Ca (OH) 2sewage pH is regulated to be 7.0-8.0, then, at every 20m 3drop into this complex micro organism fungicide 0.5-1Kg in sewage, when disposing of sewage, slowly in sewage, pass into air.
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