CN104478091A - High-efficiency ammonia nitrogen degradation composite strain culture method - Google Patents
High-efficiency ammonia nitrogen degradation composite strain culture method Download PDFInfo
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Abstract
The invention belongs to the technical field of ammonia nitrogen wastewater treatment, and particularly relates to a high-efficiency ammonia nitrogen degradation composite strain culture method. The method comprises the following steps: (1) taking ammonia nitrogen wastewater to be degraded entering a biochemical tank, filtering, decolorizing, carrying oil-out pretreatment, and diluting with distilled water to prepare 10-time, 8-time, 5-time, 2-time and 0-time wastewater diluted solutions for later use; (2) preparing a wastewater diluted solution culture medium of the ammonia nitrogen degradation composite strain; (3) preparing an ammonia nitrogen degradation composite strain solution; and (4) preserving and activating the ammonia nitrogen degradation composite strain solution. The method is mainly used in the field of treatment of high-ammonia-nitrogen wastewater.
Description
Technical field
The invention belongs to ammonia nitrogenous wastewater treatment technology field, particularly relate to a kind of cultural method of high-efficiency ammonia nitrogen degradation composite bacteria.
Background technology
High-concentration ammonia nitrogenous wastewater source is very wide and quantity discharged is large.As chemical fertilizer, coking, petrochemical industry, pharmacy, food, refuse landfill etc. all produce a large amount of high-concentration ammonia nitrogenous wastewater.A large amount of ammonia nitrogen waste water enters water body not only to be caused body eutrophication, causes black and odorous water, and will increase difficulty and the cost of process, even to crowd and biological generation toxic action.Ammonia nitrogen waste water has caused the attention of field of Environment Protection and global range on the impact of environment, at present, has carried out more research both at home and abroad to ammonia nitrogen waste water process aspect.Its research range relates to the various treatment process of biological process, physico-chemical process; As biological method, there are the methods such as nitrated and algal culture.It is referring to that the ammonia nitrogen in waste water is under the effect of various microorganism, by series reaction such as nitrification and denitrifications, finally forms nitrogen that biological process removes ammonia nitrogen, thus reaches the object removing ammonia nitrogen.The technique of biological process denitrogenation has a variety of, but mechanism is substantially identical.All need through two stages of nitrification and denitrification.Nitration reaction is nitrite or nitrate by the effect of aerobic nitrification bacterium by the ammonium oxidation in waste water under aerobic condition, comprises two primitive reaction steps: what participated in by Nitrosomas is the reaction of nitrite by mineralized nitrogen.What participated in by Nitromonas is the reaction of nitrate by Nitrite transformation.Nitrosomas and Nitromonas are all autotrophic bacterias, and they utilize the carbon source in waste water, by obtaining energy with the redox reaction of NH3-N.Reaction equation is as follows: nitrosification: 2NH
4 ++ 3O
2→ 2NO
2 -+ 2H
2o+4H
+, nitrated: 2NO
2 -+ O
2→ 2NO
3 -.Under anoxic conditions, utilize denitrifying bacteria nitrite and nitrate reduction to be overflowed from waste water for nitrogen, due to the effect of denitrifying bacteria, the nitrate produced in nitrifying process or nitrate reductase are become N
2process, be called denitrification.Electron donor in denitrification process is various organic substrates.Take methyl alcohol as carbon source be example, its reaction formula is: 6NO
3 -+ 2CH
3oH → 6NO
2 -+ 2CO
2+ 4H
2o, 6NO
2 -+ 3CH
3oH → 3N
2+ 3CO
2+ 3H
2o+6OH
-.Because the capacity usage ratio of nitrobacteria and this two classes bacterium of denitrifying bacterium is not high, therefore growth is comparatively slow, its on average for time more than 10 hours.Because its growth is comparatively slow, and it is poor to the impact tolerance of the severe factor of living environment, therefore, because the factor difference of the trophic factor in sewage and spawn culture is very large, if bacterial classification product is directly added in mud, in bacterial classification product, part bacterial classification due to discomfort, can cause the loss of microorganism resource, and remaining bacterial classification is in the laundering period, the removal ability of its ammonia nitrogen is lower, can not reach the efficient object removing ammonia nitrogen fast.Need about 40-50 days from adding mud to its processing power is stable according to use experience bacterial classification, efficiency is lower, and after system is impacted again, still needs to buy bacterial classification and the adaptive phase experiencing 40-50 days.Therefore a kind of operation technique of bacterial classification is needed at present badly, make bacterial classification can adapt to fast needed for the water quality of disposing of sewage, improve the service efficiency of bacterial classification, and good preservation means are provided, when system is impacted or occur that bacterial classification runs off, can add timely, not need to buy separately, greatly save the cost buying bacterial classification.
Summary of the invention
The object of the invention is to: the cultural method proposing a kind of efficient ammonia nitrogen composite bacteria.Use this method can shorten the onset time that ammonia nitrogen bacterial classification adds mud greatly, and through suitable preservation, can add use fast when needing use to add bacterial classification again, does not need again to tame bacterial classification again.
Technical scheme of the present invention: a kind of cultural method of high-efficiency ammonia nitrogen degradation composite bacteria, the method is made up of following step:
(1) get and enter biochemistry pool ammonia nitrogen waste water to be degraded, after filtration, decolouring, after fuel-displaced pre-treatment, be prepared into 10 times, 8 times, 5 times, 2 times and 0 times of wastewater dilution liquid with distilled water diluting, for subsequent use;
(2) prepare the substratum diluent of ammonia nitrogen degradation composite bacteria, wherein the mass percent of each component is as follows: glucose: 3-3.5%, urea: 0.6-0.8%, NaH
2pO
4: 0.05-0.1%, K
2hPO
4: 0.05-0.1%, NaNO
2: 0.05-0.1%, MgSO
4: 0.05-0.1%, CaC1
2: 0.05-0.1%, FeSO
4: 0.05-0.1%, MnSO
4: 0.05-0.1%, wastewater dilution liquid: 95-96.05%, each component percentages sum is absolutely; Wherein wastewater dilution liquid is the wastewater dilution liquid prepared in step (1), and to adjust pH be 7.5-8.0, is prepared into 10 times, 8 times, 5 times, 2 times and 0 times of wastewater dilution liquid culture medium, for subsequent use;
(3) by the mud sample in ammonia nitrogen waste water biochemistry pool to be degraded by inoculum size 3-5%, access in 10 times of wastewater dilution liquid culture mediums, make substratum mud mixed liquid, substratum mud mixed liquid is placed on shaking table, shaking speed 130-150rpm, culture temperature 30 DEG C-35 DEG C, every 10-12 hour stopping shaking table 3-4 hour, after continuously fermenting 72-84 hour; Aseptically, draw 20% fermented liquid and add in the 8 times of wastewater dilution liquid culture mediums prepared in step (2), cultural method is identical with 10 times of wastewater dilution liquid culture mediums; According to above-mentioned cultural method, use the wastewater dilution liquid culture medium of 5 times, 2 times, 0 times successively; Finally, namely obtain after 0 times of wastewater dilution liquid culture medium is cultivated for the production of ammonia nitrogen degradation composite bacteria liquid;
(4) if do not use, then step (3) cultured ammonia nitrogen degradation composite bacteria liquid is preserved under 3-6 DEG C of condition, often once need activate according to 0 times of wastewater dilution liquid culture medium cultural method in step (3) through 15-20 days simultaneously, under 3-6 DEG C of condition, preserve the ammonia nitrogen degradation composite bacteria liquid after activation.
Beneficial effect of the present invention: compared with ordinary method, substratum in this experiment employs the optimal substrates of nitrobacteria and denitrifying bacterium, and fermentation condition, nitrobacteria and denitrifying bacterium syntrophism can be ensured, on average take for the feature of duration the scheme that gradient raises adaptation target waste water step by step in conjunction with nitrobacteria and denitrifying bacterium, the time shorten of the maximum material removal rate of ammonia nitrogen 23-35 days is reached after making ammonia nitrogen bacterial strain add active sludge, and the maximum material removal rate of ammonia nitrogen improves 6-8% than conventional using method, provide a kind of preservation and activation method simultaneously, when biochemical system be subject to enormous impact need again to add bacterium liquid time, bacterial strain need not be bought separately, with the bacterium liquid preserved through overactivation after 72 hours, just can reach and add requirement.
Embodiment
The cultural method of embodiment 1, a kind of high-efficiency ammonia nitrogen degradation composite bacteria, the method is made up of following step:
(1) get and enter biochemistry pool ammonia nitrogen waste water to be degraded, after filtration, decolouring, after fuel-displaced pre-treatment, be prepared into 10 times, 8 times, 5 times, 2 times and 0 times of wastewater dilution liquid with distilled water diluting, for subsequent use;
(2) prepare the wastewater dilution liquid culture medium of ammonia nitrogen degradation composite bacteria, wherein the mass percent of each component is as follows: glucose: 3-3.5%, urea: 0.6-0.8%, NaH
2pO
4: 0.05-0.1%, K
2hPO
4: 0.05-0.1%, NaNO
2: 0.05-0.1%, MgSO
4: 0.05-0.1%, CaC1
2: 0.05-0.1%, FeSO
4: 0.05-0.1%, MnSO
4: 0.05-0.1%, wastewater dilution liquid: 95-96.05%, each component percentages sum is absolutely; Wherein wastewater dilution liquid is the wastewater dilution liquid prepared in step (1), and to adjust pH be 7.5-8.0, is prepared into 10 times, 8 times, 5 times, 2 times and 0 times of wastewater dilution liquid culture medium, for subsequent use;
(3) by the mud sample in ammonia nitrogen waste water biochemistry pool to be degraded by inoculum size 3-5%, access in 10 times of wastewater dilution liquid culture mediums, make substratum mud mixed liquid, substratum mud mixed liquid is placed on shaking table, shaking speed 130-150rpm, culture temperature 30 DEG C-35 DEG C, every 10-12 hour stopping shaking table 3-4 hour, after continuously fermenting 72-84 hour; Aseptically, draw 20% fermented liquid and add in the 8 times of wastewater dilution liquid culture mediums prepared in step (2), cultural method is identical with 10 times of wastewater dilution liquid culture mediums; According to above-mentioned cultural method, use the wastewater dilution liquid culture medium of 5 times, 2 times, 0 times successively; Finally, namely obtain after 0 times of wastewater dilution liquid culture medium is cultivated for the production of ammonia nitrogen degradation composite bacteria liquid;
(4) if do not use, then step (3) cultured ammonia nitrogen degradation composite bacteria liquid is preserved under 3-6 DEG C of condition, often once need activate according to 0 times of wastewater dilution liquid culture medium cultural method in step (3) through 15-20 days simultaneously, under 3-6 DEG C of condition, preserve the ammonia nitrogen degradation composite bacteria liquid after activation.
Concrete practice is verified:
One, get certain fertilizer plant's sewage, analyze after testing, COD is 4214mg/L, TN is 217.2mg/L, after filtration, decolouring after, use respectively distilled water diluting 10 times, 8 times, 5 times, 2 times for subsequent use.This group solution is target wastewater dilution liquid.
(2) the substratum mass percent preparing ammonia nitrogen degradation composite bacteria is as follows: glucose: 3%, urea: 0.6%, NaH
2pO
4: 0.05%, K
2hPO
4: 0.05%, NaNO
2: 0.05%, MgSO
4: 0.05%, CaC1
2: 0.05%, FeSO
4: 0.05%, MnSO
4: 0.05%, wastewater dilution liquid: 96.05%.Wherein wastewater dilution liquid is the wastewater dilution liquid prepared in step (1), is prepared into 10 times, 8 times, 5 times, 2 times and 0 times of wastewater dilution liquid culture medium, adjustment pH=7.5, for subsequent use.
(3) mud sample of the biochemistry pool of the high ammonia-nitrogen wastewater of operational excellence is pressed inoculum size 3%, access in 10 times of wastewater dilution liquid culture mediums, make substratum mud mixed liquid, solution is placed on shaking table, shaking speed 130rpm, culture temperature 30 DEG C, 4 hours are stopped every 10 hours shaking tables, aseptically draw 20% fermented liquid after continuously fermenting 72 hours and add 8 times of wastewater dilution liquid culture mediums to, cultural method is identical with 10 times of wastewater dilution liquid culture mediums, uses the culture medium culturing of the wastewater dilution liquid of 5 times, 2 times, 0 times successively.
(4) just can obtain growing fast under this kind of wastewater environment in the nutrient solution after 0 times of wastewater dilution liquid culture medium is cultivated and the time of the whole domestication cultivation of active high-efficiency ammonia nitrogen degradation composite bacteria is preferably 17 days.
(5) treated ammonia nitrogen bacterial classification is joined in the mud of the biochemical section of water treatment, contrasts as follows with ordinary method:
(6) preserve under the bacterium liquid obtained through this method process being put into 3-6 degree, need to activate after preserving through 15 days, use the substratum in (2), the biochemistry pool water inlet of wastewater dilution liquid replaces, inoculum size 20% is after the cultural method in (3) cultivates 72 hours, the bacterium liquid obtained directly is added to active sludge, and the data in result of use and upper table are basically identical.
(5) the fertilizer plant's waste water got is after ordinary method process, and after 50 days, ammonia nitrogen removal frank is up to 87.8%.This cultural method is cultivated the composite bacteria obtained be linked into time chemical fertilizer Sewage Plant active sludge in, just 95.6% is reached and clearance is more stable through 3 day later stage NH_3-N treating efficiency, greatly save ammonia nitrogen bacterial classification and directly put into adaptation time in mud, and when needing to reuse, only need the time of 3 days just directly can be added to bacterial classification in mud.
Two, get certain coking of coal sewage work, analyze after testing, COD is 3762mg/L, TN is 194.4mg/L, after filtration, decolouring after, use respectively distilled water diluting 10 times, 8 times, 5 times, 2 times for subsequent use.This group solution is target wastewater dilution liquid.
(2) prepare ammonia nitrogen degradation composite bacteria substratum mass percent as follows: glucose: 3.5%, urea: 0.8%, NaH
2pO
4: 0.1%, K
2hPO
4: 0.1%, NaNO
2: 0.1%, MgSO
4: 0.1%, CaC1
2: 0.1%, FeSO
4: 0.1%, MnSO
4: 0.1%, wastewater dilution liquid: 95%.Wherein wastewater dilution liquid is the wastewater dilution liquid prepared in step (1), is prepared into 10 times, 8 times, 5 times, 2 times and 0 times of wastewater dilution liquid culture medium, adjustment pH=8.0, for subsequent use.
(3) mud sample of the biochemistry pool of the high ammonia-nitrogen wastewater of operational excellence is pressed inoculum size 5%, access in 10 times of wastewater dilution liquid culture mediums, make substratum mud mixed liquid, solution is placed on shaking table, shaking speed 150rpm, culture temperature 30 DEG C, 4 hours are stopped every 10 hours shaking tables, aseptically draw 20% fermented liquid after continuously fermenting 72 hours and add 8 times of wastewater dilution liquid culture mediums to, cultural method is identical with 10 times of wastewater dilution liquid culture mediums, uses the wastewater dilution liquid culture medium of 5 times, 2 times, 0 times to cultivate successively.
(4) just can obtain growing fast under this kind of wastewater environment in the nutrient solution after 0 times of wastewater dilution liquid culture medium is cultivated and the time of the whole domestication cultivation of active high-efficiency ammonia nitrogen degradation composite bacteria is preferably 15 days.
(5) treated ammonia nitrogen bacterial classification is joined in the mud of the biochemical section of water treatment, contrasts as follows with ordinary method:
(6) preserve under the bacterium liquid obtained through this method process being put into 3-6 degree, need to activate after preserving through 15 days, use the substratum in (2), the biochemistry pool water inlet of wastewater dilution liquid replaces, inoculum size 20% is after the cultural method in (3) cultivates 72 hours, the bacterium liquid obtained directly is added to active sludge, and the data in result of use and upper table are basically identical.
(5) the fertilizer plant's waste water got is after ordinary method process, and after 50 days, ammonia nitrogen removal frank is up to 88.5%.This cultural method is cultivated the composite bacteria obtained be linked into time chemical fertilizer Sewage Plant active sludge in, just 96.7% is reached and clearance is more stable through 3 day later stage NH_3-N treating efficiency, greatly save ammonia nitrogen bacterial classification and directly put into adaptation time in mud, and when needing to reuse, only need the time of 3 days just directly can be added to bacterial classification in mud.
Claims (1)
1. a cultural method for high-efficiency ammonia nitrogen degradation composite bacteria, is characterized in that: the method is made up of following step:
(1) get and enter biochemistry pool ammonia nitrogen waste water to be degraded, after filtration, decolouring, after fuel-displaced pre-treatment, be prepared into 10 times, 8 times, 5 times, 2 times and 0 times of wastewater dilution liquid with distilled water diluting, for subsequent use;
(2) prepare the wastewater dilution liquid culture medium of ammonia nitrogen degradation composite bacteria, wherein the mass percent of each component is as follows: glucose: 3-3.5%, urea: 0.6-0.8%, NaH
2pO
4: 0.05-0.1%, K
2hPO
4: 0.05-0.1%, NaNO
2: 0.05-0.1%, MgSO
4: 0.05-0.1%, CaC1
2: 0.05-0.1%, FeSO
4: 0.05-0.1%, MnSO
4: 0.05-0.1%, wastewater dilution liquid: 95-96.05%, each component percentages sum is absolutely; Wherein wastewater dilution liquid is the wastewater dilution liquid prepared in step (1), and to adjust pH be 7.5-8.0, is prepared into 10 times, 8 times, 5 times, 2 times and 0 times of wastewater dilution liquid culture medium, for subsequent use;
(3) by the mud sample in ammonia nitrogen waste water biochemistry pool to be degraded by inoculum size 3-5%, access in 10 times of wastewater dilution liquid culture mediums, make substratum mud mixed liquid, substratum mud mixed liquid is placed on shaking table, shaking speed 130-150rpm, culture temperature 30 DEG C-35 DEG C, every 10-12 hour stopping shaking table 3-4 hour, after continuously fermenting 72-84 hour; Aseptically, draw 20% fermented liquid and add in the 8 times of wastewater dilution liquid culture mediums prepared in step (2), cultural method is identical with 10 times of wastewater dilution liquid culture mediums; According to above-mentioned cultural method, use the wastewater dilution liquid culture medium of 5 times, 2 times, 0 times successively; Finally, namely obtain after 0 times of wastewater dilution liquid culture medium is cultivated for the production of ammonia nitrogen degradation composite bacteria liquid;
(4) if do not use, then step (3) cultured ammonia nitrogen degradation composite bacteria liquid is preserved under 3-6 DEG C of condition, often once need activate according to 0 times of wastewater dilution liquid culture medium cultural method in step (3) through 15-20 days simultaneously, under 3-6 DEG C of condition, preserve the ammonia nitrogen degradation composite bacteria liquid after activation.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104785216A (en) * | 2015-04-09 | 2015-07-22 | 西南科技大学 | Preparation method of hypha/nanoparticle composite sphere material |
| CN104830740A (en) * | 2015-05-28 | 2015-08-12 | 陕西科技大学 | Preparation and application methods of efficient microorganism agent for treating compound fertilizer wastewater |
| CN105858894A (en) * | 2016-02-19 | 2016-08-17 | 南昌大学 | Method for nitrogen abundance transformation treatment of wastewater with high ammonia nitrogen |
| CN106947695A (en) * | 2017-04-17 | 2017-07-14 | 西安华诺环保股份有限公司 | The cultural method of ammonia nitrogen degradation bacterium |
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