CN111996133A - Method for biologically enhancing application of sulfate reducing bacteria - Google Patents

Method for biologically enhancing application of sulfate reducing bacteria Download PDF

Info

Publication number
CN111996133A
CN111996133A CN201910443305.2A CN201910443305A CN111996133A CN 111996133 A CN111996133 A CN 111996133A CN 201910443305 A CN201910443305 A CN 201910443305A CN 111996133 A CN111996133 A CN 111996133A
Authority
CN
China
Prior art keywords
reducing bacteria
urease
culture
sulfate
sulfate reducing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910443305.2A
Other languages
Chinese (zh)
Inventor
张献波
郑连爽
康杰卿
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Whibop Wuhan Bio Environmental Protection Technology Co ltd
Original Assignee
Whibop Wuhan Bio Environmental Protection Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Whibop Wuhan Bio Environmental Protection Technology Co ltd filed Critical Whibop Wuhan Bio Environmental Protection Technology Co ltd
Priority to CN201910443305.2A priority Critical patent/CN111996133A/en
Publication of CN111996133A publication Critical patent/CN111996133A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/20—Heavy metals or heavy metal compounds

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Medicinal Chemistry (AREA)
  • Water Supply & Treatment (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Soil Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a method for biologically strengthening application of sulfate reducing bacteria, which is characterized in that urease-producing strains are adopted to strengthen the capability of fixing heavy metals, mixed bacteria culture solution is added into cadmium-containing wastewater, and sealed culture is carried out to obtain treated CdS precipitate. The urease-producing strain adopted by the invention has a paragenetic relationship with sulfate reducing bacteria, and can consume O while participating in microbial precipitation together to improve the efficiency of solidifying heavy metals2Providing a desired anaerobic environment for the SRB strain while simultaneouslyIn the inhibition of sulfide ion S2‑Oxidizing bacteria growth, reducing H in bacteria liquid after culture2S causes environmental pollution. In the presence of 20mg/L CdCl2The cadmium removal rate of the waste liquid reaches 99.0 percent, has good cadmium removal effect, and can be widely applied to the treatment of water sources and soil containing heavy metals.

Description

Method for biologically enhancing application of sulfate reducing bacteria
The technical field is as follows:
the invention belongs to the technical field of environmental microorganisms, and particularly relates to a method for biologically enhancing application of sulfate reducing bacteria.
Background art:
at present, with the rapid development of social economy, the enrichment of heavy metal elements in the environment is increasingly intensified, and human activities such as mineral resource development, metal processing and smelting, chemical production, factory discharge, sewage irrigation and the like gradually evolve into main sources of heavy metal pollution. Excessive heavy metal is discharged into the environment, so that the soil productivity is reduced, meanwhile, the human health is threatened, for example, Cd is accumulated in a human body through a food chain to damage the immune system, the urinary system, bones, the nervous system, the reproductive system and the like of the human body, and meanwhile, Cd also has strong carcinogenic, teratogenic and mutagenic effects. With the continuous development of social economy, environmental problems are more and more emphasized, and the problem of soil pollution, particularly the soil pollution caused by heavy metal Cd, becomes a research hotspot in the fields of current agriculture, ecology and environmental science.
The microbial technology treatment has the advantages of short restoration growth period, rapid growth, good restoration effect, no secondary pollution, easy management and operation, small investment and the like. The sulfate reducing bacteria adopted by the invention can neutralize SO under the conditions of anaerobism and addition of a small amount of carbon source4 2-Reduction to H2S, combining heavy metals in the soil to form inactive metal sulfide (with solubility product constant of 2 x 10, such as CuS, PbS, CdS and ZnS)-37～10-20.4) So as to reduce the absorption and utilization of animals and plants and achieve the aim of repairing heavy metal pollution.
At present, because the use environment of sulfate reducing bacteria is limited, most of the sulfate reducing bacteria are combined with immobilization, and the sulfate reducing bacteria are developed into a widely applied technology:
in CN 106636057A, polyvinyl alcohol and sodium alginate are used as carriers, sodium lactate is used as a nutrient source, and the sulfate reducing bacteria immobilized pellet is prepared and has a good effect of removing heavy metals in sediments.
In CN 106861654A, sulfate reducing bacteria sludge is added into gel prepared from polyvinyl alcohol and sodium alginate, and immobilized particles are obtained by crosslinking, and the immobilized particles can be used for treating acid mine wastewater.
However, the monosulfate reducing bacteria have obvious defects in the practical application process, such as difficult growth of the immobilized sulfate reducing bacteria and application processIn the middle of the production of pungent odor (H)2S), causing secondary potential pollution, etc.
In the treatment of heavy metal-containing wastewater and soil pollution, researchers continuously find that a plurality of biological processes can not be completed or can only be performed weakly by a single strain of microorganism, but symbiosis, co-culture and co-honor are carried out under the condition that two or more microorganisms coexist, so that the action of the microorganisms develops towards the direction of generating beneficial effects, and the optimized biochemical process is completed.
Compared with the prior art, the invention strengthens the current use situation of the single strain of the sulfate reducing bacteria, realizes the complementary pairing of the advantages of the mixed strains by adding the urease-producing strain, and consumes O when the urease-producing strain grows2Providing a prospective anaerobic environment for SRB strains while inhibiting sulfur ions S2-Oxidizing bacteria growth, reducing H in bacteria liquid after culture2The content of S is increased2-Concentration, reduction of H2And the environmental pollution caused by S promotes the whole microbial precipitation and crystallization process.
The invention content is as follows:
the invention aims to strengthen the defects of sulfate reducing bacteria in heavy metal pollution treatment and reduce the generation of H under the action of the sulfate reducing bacteria2S, the applicability of the sulfate reducing bacteria is improved, and the capability of removing heavy metals (such as cadmium) is enhanced.
The invention provides a method for introducing urease-producing bacterial strains to strengthen sulfate reducing bacteria, which adopts the following technical scheme:
(1) preparing mixed bacteria liquid: adding the culture solution inoculated with the sulfate reducing bacteria and the urease-producing strain into a biological strengthening culture medium for culture to obtain a mixed culture solution of the sulfate reducing bacteria and the urease-producing strain.
(2) Wherein the urease-producing strain belongs to Sporosarcina pasteurii, and the sulfate reducing bacteria is vibrio devulcani.
(3) The sulfate reducing bacteria culture solution comprises the following components: 0.3 to 0.5g/L KH2PO4，0.05~0.1 g/L MgCl2·6H2O, 2.0-3.0 g/L sodium lactate, 0.03-0.05 g/L CaCl2，1.5~2.5 g/L NaCl，1.0~2.0 g/L NH4Cl，2.0~3.0 g/L Na2SO40.2 to 0.3g/L ascorbic acid. The urease-producing strain culture solution comprises the following components: 1.0-2.0 g/L peptone, 2.0-3.0 g/L NaCl, 2.0-2.5 g/L KH2PO41.0-3.0 g/L urea and 0.5-1.5 g/L glucose. The biological strengthening medium comprises the following components: 1.5-3.0 g/L KH2PO4，2.5~4.0 g/L NaCl，1.0 ~2.5g/LNH4Cl, 0.5-1.0 g/L glucose, 2.0-4.0 g/L sodium lactate, 0.5-1.5 g/L peptone, 1.0-2.5 g/L urea, 1.5-3.0 g/L Na2SO4，0.05~0.1 g/L MgCl2·6H2O，0.05 ~0.1g/L CaCl20.1 to 0.5g/L ascorbic acid.
The pH of the culture solution is 6.5-7.5.
(4) The activated culture conditions of the sulfate reducing bacteria are 30-35 ℃ and culture in a constant temperature oscillator of 70-90 r/min for 24 hours. The activating culture condition of the urease-producing strain is 30-35 ℃, and the urease-producing strain is cultured in a constant temperature oscillator of 95-120 r/min for 24 hours.
(5) The culture conditions of the biological strengthening culture medium are 30-35 ℃ and culture in a constant temperature oscillator of 60-85 r/min for 24 h.
(6) Adding 1-5% of activated sulfate reducing bacteria culture solution and 1-2% of activated urease-producing strain to form a mixed strain, wherein the volume ratio of the sulfate reducing bacteria culture solution to the urease-producing strain culture solution is 1-2: 1.
Compared with the prior art, the invention has the following advantages:
(1) the urease-producing strain is used to strengthen the application of sulfate reducing bacteria, and the urease-producing strain is used as oxygen consuming strain to consume O in soil2Continuously reducing ORP value in soil environment, reducing ORP in environment to be less than-100 mv, leading SRB strain to grow rapidly,
(2) stabilizing pH in soil environment, increasing pH in environment by urease-producing strain metabolism, and reducing SRB strain SO4 2-Reduction to S2-In the process of (3), the inhibition of the growth of the SRB strain due to the reduction of pH is reduced;
(3) the urease-producing strain maintains a neutral or weak alkaline environment in the environment, is also beneficial to inhibiting the metabolism of acidophilic microorganisms such as protobescens, phlebotomium and the like in soil, and generates reductive sulfides by the SRB strain to be converted into sulfates again.
(4) Meanwhile, the pH is adjusted to be alkalescent (7.0-7.5), and H in the cultured bacterial liquid can be effectively reduced2Content of S, control of H2S produces stink, reduces environmental pollution and S2-Conversion to H in acidic Environment2S。
Description of the drawings:
in order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, and the drawings in the following description are some implementations of the present invention.
FIG. 1 shows the treatment of 20mg/L Cd by a mixed strain of sulfate-reducing bacteria and urease-producing strains in example 12+Efficiency.
FIG. 2 is an SEM image of CdS precipitates obtained from a mixed strain of sulfate-reducing bacteria and urease-producing strains under the conditions of example 1.
The specific implementation scheme is as follows:
the invention provides a method for biologically strengthening application of sulfate reducing bacteria, which aims to strengthen the current use situation of single strains of the sulfate reducing bacteria at present, realizes the complementary pairing of the advantages of mixed strains by adding urease-producing strains, and consumes O when the urease-producing strains grow2Providing a prospective anaerobic environment for SRB strains while inhibiting sulfur ions S2-Oxidizing bacteria growth, reducing H in bacteria liquid after culture2The content of S.
In the same Cd compared to single strain sulfate-reducing bacteria2+Under the environment, precipitation is generated by sulfate reducing bacteria enhanced by urease-producing strains, and Cd is removed2+Efficiency is improved by 10-20%. The invention is mainly characterized in that the sulfate reducing bacteria are strengthened by adding the urease-producing bacterial strains, and the complementary pairing of the advantages of the mixed bacterial strains is realized. The urease-producing strain has short growth period, high oxygen consumption and capacity of consuming O in environment preferentially2And the low ORP environment is kept, and the growth of sulfate reducing bacteria is facilitated. The sulfate reducing bacteria can react SO under anaerobic condition4 2-Reduction to S2-In active state with respect to the environmentCd2+And the heavy metal ions are combined and consolidated, so that the aim of removing the heavy metals in the soil and the water body is fulfilled.
The invention relates to a sulfate biological enhancement application method, which specifically comprises the following steps:
(1) selecting a single colony of sulfate reducing bacteria, inoculating the single colony in 200ml of sterilized culture medium for activated culture, wherein the sulfate reducing bacteria culture solution comprises the following components: 0.3 to 0.5g/L KH2PO4，0.05~0.1 g/L MgCl2·6H2O, 2.0-3.0 g/L sodium lactate, 0.03-0.05 g/L CaCl2，1.5~2.5 g/L NaCl，1.0~2.0 g/L NH4Cl，2.0~3.0 g/L Na2SO40.2 to 0.3g/L ascorbic acid, and a pH of 6.5 to 7.5. The activated culture conditions of the sulfate reducing bacteria are 30-35 ℃ and culture in a constant temperature oscillator of 70-90 r/min for 24 hours.
(2) Selecting a single colony of sulfate reducing bacteria, inoculating the single colony in 150ml of sterilized culture medium for activated culture, wherein the urease-producing strain culture solution comprises the following components: 1.0-2.0 g/L peptone, 2.0-3.0 g/L NaCl, 2.0-2.5 g/L KH2PO41.0-3.0 g/L of urea, 0.5-1.5 g/L of glucose and pH of 6.5-7.5, and culturing the urease-producing strain in a constant-temperature oscillator at the temperature of 30-35 ℃ and the speed of 95-120 r/min for 24 hours.
(3) A certain proportion of sulfate reducing bacteria and urease-producing strains are mixed and added into 150ml of sterilized culture medium for activation culture. The biological strengthening medium comprises the following components: 1.5-3.0 g/L KH2PO4，2.5~4.0 g/L NaCl，1.0 ~2.5g/LNH4Cl, 0.5-1.0 g/L glucose, 2.0-4.0 g/L sodium lactate, 0.5-1.5 g/L peptone, 1.0-2.5 g/L urea, 1.5-3.0 g/L Na2SO4，0.05~0.1 g/L MgCl2·6H2O，0.05 ~0.1g/L CaCl20.1 to 0.5g/L ascorbic acid, and a pH of 6.5 to 7.5. The culture conditions of the biological strengthening culture medium are 30-35 ℃ and culture in a constant temperature oscillator of 60-85 r/min for 24 h.
The specific application of the above process is illustrated by the following examples.
Example 1
A sulfate reducing bacteria biological strengthening application method comprises the following steps:
(1) preparing a biological strengthening culture medium: adding 1.5g KH into 1L distilled water2PO42.5g/L NaCl, 2.0g/L sodium lactate, 0.5g/L peptone, 1.5g/L Na2SO4，0.05 g/L MgCl2·6H2O，0.05g/L CaCl2. The pH was adjusted to 7.0 by addition of 1mol/L NaOH. Sterilizing the culture medium at 121 deg.C for 20min, and cooling.
(2) Taking 0.5g of glucose and 1.0g of NH4UV-Sterilization of Cl, 0.1g ascorbic acid, 1.0g Urea alone for 30min, 20mg CdCl2Added to the above prepared 1L solution.
(3) Taking culture solution of sulfate reducing bacteria and urease-producing strains according to the volume ratio of 1:1, simultaneously adding two microorganisms into the biological enhanced culture solution, wherein the total volume of the bacteria solution accounts for 2% of the volume of the biological enhanced culture solution, sealing and culturing in a sealed manner.
(4) Culturing the mixed bacteria liquid in a constant temperature oscillator at 30-35 ℃ and 60-85 r/min for 24h, and measuring Cd in the culture medium by adopting a flame atomic absorption spectrophotometry2+And (4) content.
Example 2
(1) Preparing a biological strengthening culture medium: adding 1.5g KH into 1L distilled water2PO42.5g/L NaCl, 2.0g/L sodium lactate, 0.5g/L peptone, 1.5g/L Na2SO4，0.05 g/L MgCl2·6H2O，0.05g/L CaCl2. The pH was adjusted to 7.0 by addition of 1mol/L NaOH. Sterilizing the culture medium at 121 deg.C for 20min, and cooling.
(2) Taking 0.5g of glucose and 1.0g of NH4UV-Sterilization of Cl, 0.1g ascorbic acid, 1.0g Urea alone for 30min, 20mg CdCl2Added to the above prepared 1L solution.
(3) Taking a culture solution of sulfate reducing bacteria and urease-producing strains according to the volume ratio of 1:1, adding the urease-producing strains for 3 hours, adding the sulfate reducing bacteria, sealing and culturing, wherein the total volume of the bacteria solution accounts for 2% of the volume of the biological enhanced culture solution.
(4) Culturing the mixed bacteria liquid in a constant temperature oscillator at 30-35 ℃ and 60-85 r/min for 24h, and measuring by adopting a flame atomic absorption spectrophotometryCd in culture medium2+And (4) content.
Example 3
(1) Preparing a biological strengthening culture medium: adding 1.5g KH into 1L distilled water2PO42.5g/L NaCl, 2.0g/L sodium lactate, 0.5g/L peptone, 1.5g/L Na2SO4，0.05 g/L MgCl2·6H2O，0.05g/L CaCl2. The pH was adjusted to 7.0 by addition of 1mol/L NaOH. Sterilizing the culture medium at 121 deg.C for 20min, and cooling.
(2) Taking 0.5g of glucose and 1.0g of NH4UV-Sterilization of Cl, 0.1g ascorbic acid, 1.0g Urea alone for 30min, 20mg CdCl2Added to the above prepared 1L solution.
(3) Taking a culture solution of sulfate reducing bacteria and a urease-producing strain according to the volume ratio of 1:1, adding the sulfate reducing bacteria into the culture solution for 3 hours, adding the urease-producing strain into the culture solution, wherein the total volume of the culture solution accounts for 2 percent of the volume of the biological enhanced culture solution, sealing and culturing the culture solution in a sealed manner.
(4) Culturing the mixed bacteria liquid in a constant temperature oscillator at 30-35 ℃ and 60-85 r/min for 24h, and measuring Cd in the culture medium by adopting a flame atomic absorption spectrophotometry2+And (4) content.
Example 4
(1) Preparing a biological strengthening culture medium: adding 1.5g KH into 1L distilled water2PO42.5g/L NaCl, 2.0g/L sodium lactate, 0.5g/L peptone, 1.5g/L Na2SO4，0.05 g/L MgCl2·6H2O，0.05g/L CaCl2. The pH was adjusted to 7.0 by addition of 1mol/L NaOH. Sterilizing the culture medium at 121 deg.C for 20min, and cooling.
(2) Taking 0.5g of glucose and 1.0g of NH4UV-Sterilization of Cl, 0.1g ascorbic acid, 1.0g Urea alone for 30min, 20mg CdCl2Added to the above prepared 1L solution.
(3) Taking culture solution of sulfate reducing bacteria and urease-producing strains according to the volume ratio of 2:1, simultaneously adding two microorganisms into the biological enhanced culture solution, wherein the total volume of the bacteria solution accounts for 2% of the volume of the biological enhanced culture solution, sealing and culturing in a sealed manner.
(4) Mixing the bacterial liquid at 30-35 ℃ and 60-85 r/miCulturing in constant temperature oscillator for 24h, and measuring Cd in culture medium by flame atomic absorption spectrophotometry2+And (4) content.
Example 5
(1) Preparing a biological strengthening culture medium: adding 1.5g KH into 1L distilled water2PO42.5g/L NaCl, 2.0g/L sodium lactate, 0.5g/L peptone, 1.5g/L Na2SO4，0.05 g/L MgCl2·6H2O，0.05g/L CaCl2. The pH was adjusted to 7.0 by addition of 1mol/L NaOH. Sterilizing the culture medium at 121 deg.C for 20min, and cooling.
(2) Taking 0.5g of glucose and 1.0g of NH4UV-Sterilization of Cl, 0.1g ascorbic acid, 1.0g Urea alone for 30min, 20mg CdCl2Added to the above prepared 1L solution.
(3) Taking culture solution of sulfate reducing bacteria and urease-producing strains according to the volume ratio of 1:2, simultaneously adding two microorganisms into the biological enhanced culture solution, wherein the total volume of the bacteria solution accounts for 2% of the volume of the biological enhanced culture solution, sealing and culturing in a sealed manner.
(4) Culturing the mixed bacteria liquid in a constant temperature oscillator at 30-35 ℃ and 60-85 r/min for 24h, and measuring Cd in the culture medium by adopting a flame atomic absorption spectrophotometry2+And (4) content.

Claims (6)

1. A method for biologically enhancing application of sulfate reducing bacteria is characterized by comprising the following steps: adding the culture solution inoculated with the sulfate reducing bacteria and the urease-producing strain into a biological strengthening culture medium for culture to obtain a mixed culture solution of the sulfate reducing bacteria and the urease-producing strain.
2. The method of fortifying sulfate-reducing bacteria with urease-producing strains according to claim 1, wherein: the sulfate reducing bacteria culture solution comprises the following components: 0.3 to 0.5g/L KH2PO4，0.05~0.1 g/L MgCl2·6H2O, 2.0-3.0 g/L sodium lactate, 0.03-0.05 g/L CaCl2，1.5~2.5 g/L NaCl，1.0~2.0 g/L NH4Cl，2.0~3.0 g/L Na2SO40.2-0.3 g/L ascorbic acid; the urease-producing strain culture solution comprises the following components: 1.0-2.0 g/L peptone, 2.0-3.0 g/L NaCl, 2.0-2.5 g/L KH2PO41.0-3.0 g/L urea and 0.5-1.5 g/L glucose; the biological strengthening medium comprises the following components: 1.5-3.0 g/L KH2PO4，2.5~4.0 g/L NaCl，1.0 ~2.5g/LNH4Cl, 0.5-1.0 g/L glucose, 2.0-4.0 g/L sodium lactate, 0.5-1.5 g/L peptone, 1.0-2.5 g/L urea, 1.5-3.0 g/L Na2SO4，0.05~0.1 g/L MgCl2·6H2O，0.05 ~0.1g/L CaCl20.1-0.5 g/L ascorbic acid; the pH of the culture solution is 6.5-7.5.
3. The method of claim 1, wherein the sulfate-reducing bacteria are used for bioaugmentation in a biological enhancement system comprising: the activation culture condition of the sulfate reducing bacteria is 30-35 ℃, and the sulfate reducing bacteria are cultured in a constant temperature oscillator of 70-90 r/min for 24 hours; the activating culture condition of the urease-producing strain is 30-35 ℃, and the urease-producing strain is cultured in a constant temperature oscillator of 95-120 r/min for 24 hours.
4. The method of claim 1, wherein the sulfate-reducing bacteria are used for bioaugmentation in a biological enhancement system comprising: the culture conditions of the biological strengthening culture medium are 30-35 ℃ and culture in a constant temperature oscillator of 60-85 r/min for 24 h.
5. The method of claim 4, wherein the sulfate-reducing bacteria are used for bioaugmentation in a biological enhancement system comprising: adding 1-5% of activated sulfate reducing bacteria culture solution and 1-2% of activated urease-producing strain to form a mixed strain, wherein the volume ratio of the sulfate reducing bacteria culture solution to the urease-producing strain culture solution is 1-2: 1.
6. The method of claim 1, wherein the sulfate-reducing bacteria are used for bioaugmentation in a biological enhancement system comprising: the urease-producing strain is mixed with sulfate reducing bacteria for culture and is used mainly in treating waste water, soil, etc. containing heavy metal with strengthened sulfate reducing bacteria.
CN201910443305.2A 2019-05-27 2019-05-27 Method for biologically enhancing application of sulfate reducing bacteria Pending CN111996133A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910443305.2A CN111996133A (en) 2019-05-27 2019-05-27 Method for biologically enhancing application of sulfate reducing bacteria

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910443305.2A CN111996133A (en) 2019-05-27 2019-05-27 Method for biologically enhancing application of sulfate reducing bacteria

Publications (1)

Publication Number Publication Date
CN111996133A true CN111996133A (en) 2020-11-27

Family

ID=73461799

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910443305.2A Pending CN111996133A (en) 2019-05-27 2019-05-27 Method for biologically enhancing application of sulfate reducing bacteria

Country Status (1)

Country Link
CN (1) CN111996133A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110078209A (en) * 2019-05-16 2019-08-02 桂林电子科技大学 A kind of method of micro-reduction sulfate formation cadmium sulphur mine
CN113351641A (en) * 2021-06-23 2021-09-07 西南科技大学 Method for remedying heavy metal pollution of smelting slag through non-covering organisms
CN114951245A (en) * 2022-05-27 2022-08-30 广东桃林生态环境有限公司 Method for preventing surface water of heavy metal mining waste land from seeping downwards and application of method in treatment of heavy metal mining waste land
CN114985447A (en) * 2022-06-02 2022-09-02 中国地质大学（北京） Microbial geochemical in-situ mineralization method for synchronously solidifying multiple heavy metals of nonferrous metal mine
CN115261277A (en) * 2022-08-11 2022-11-01 成都润世动源科技有限公司 Use method of biological ammonia preparation for treating acid mine wastewater by sulfate reducing bacteria

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103289920A (en) * 2012-04-23 2013-09-11 清华大学 Urease-producing microbes and curing method for heavy metals in foundation
CN104450552A (en) * 2014-08-17 2015-03-25 西北大学 Sulfate reducing bacteria-phosphate solubilizing bacteria and application thereof in combined remediation of cadmium contaminated soil
CN105838702A (en) * 2016-05-05 2016-08-10 中国海洋大学 Urease-producing strain immobilization and application of urease-producing strain
CN107287129A (en) * 2016-04-01 2017-10-24 兰州大学 One plant can make heavy metal settle sulfate reducing bacteria and its application
CN107446961A (en) * 2017-09-08 2017-12-08 太原理工大学 A kind of method that sulfate reducing bacteria converts for mediation reinforcement sludge carbon source

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103289920A (en) * 2012-04-23 2013-09-11 清华大学 Urease-producing microbes and curing method for heavy metals in foundation
CN104450552A (en) * 2014-08-17 2015-03-25 西北大学 Sulfate reducing bacteria-phosphate solubilizing bacteria and application thereof in combined remediation of cadmium contaminated soil
CN107287129A (en) * 2016-04-01 2017-10-24 兰州大学 One plant can make heavy metal settle sulfate reducing bacteria and its application
CN105838702A (en) * 2016-05-05 2016-08-10 中国海洋大学 Urease-producing strain immobilization and application of urease-producing strain
CN107446961A (en) * 2017-09-08 2017-12-08 太原理工大学 A kind of method that sulfate reducing bacteria converts for mediation reinforcement sludge carbon source

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
刘广民等: "产酸-硫酸盐还原系统中产酸菌的发酵类型及其与SRB的协同作用", 《环境科学学报》 *
李萌等: "巴氏芽孢八叠球菌诱变选育脲酶高产菌株", 《中国农业科技导报》 *
王继勇等: "一株产脲酶菌株的分离及其对Cd~(2+)的去除研究", 《环境科学学报》 *
金鑫等: "铁氧化物对硫酸盐还原菌分解硫酸盐矿物的协同作用", 《矿物学报》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110078209A (en) * 2019-05-16 2019-08-02 桂林电子科技大学 A kind of method of micro-reduction sulfate formation cadmium sulphur mine
CN113351641A (en) * 2021-06-23 2021-09-07 西南科技大学 Method for remedying heavy metal pollution of smelting slag through non-covering organisms
CN114951245A (en) * 2022-05-27 2022-08-30 广东桃林生态环境有限公司 Method for preventing surface water of heavy metal mining waste land from seeping downwards and application of method in treatment of heavy metal mining waste land
CN114985447A (en) * 2022-06-02 2022-09-02 中国地质大学（北京） Microbial geochemical in-situ mineralization method for synchronously solidifying multiple heavy metals of nonferrous metal mine
CN115261277A (en) * 2022-08-11 2022-11-01 成都润世动源科技有限公司 Use method of biological ammonia preparation for treating acid mine wastewater by sulfate reducing bacteria

Similar Documents

Publication Publication Date Title
CN111996133A (en) Method for biologically enhancing application of sulfate reducing bacteria
CN111620444B (en) Method and system for biological treatment of acid mine wastewater and recovery of iron ions
CN106833674A (en) A kind of heavy-metal contaminated soil renovation agent preparation method
CN106587197B (en) Nano biological circulating water treatment agent
CN102603064B (en) A kind of method of Nitrogen-and Phosphorus-containing sewage synchronous denitrification dephosphorizing
CN104891650A (en) Rapid culture method of simultaneous desulfidation and denitrogenation granular sludge
CN104830740A (en) Preparation and application methods of efficient microorganism agent for treating compound fertilizer wastewater
CN102583770A (en) Bamboo charcoal-photosynthetic bacteria integrated municipal sanitary wastewater treating agent
CN102776140B (en) Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof
CN113215050A (en) Algae-bacterium symbiotic composite microbial preparation for sewage treatment and preparation method thereof
CN101054242A (en) Application of planococcus psychrotoleratus in treating sewage at low temperature
CN109019874B (en) Biological growth promoter for papermaking wastewater and preparation method thereof
CN112320928A (en) Method for treating pickling wastewater by using activated sludge-dunaliella salina mixture
CN104388342B (en) A kind of short distance nitration bacterium pseudomonad and application
CN114149087B (en) Method for treating arsenic-containing waste liquid by agricultural waste in cooperation with microorganisms
CN112358041B (en) Granular sludge culture method for synchronous denitrification and methane production and COD removal
CN101054241A (en) Application of flavobacterium omnivorum in treating sewage at low temperature
CN106190896B (en) Artificial photosynthetic denitrification denitrogenation microbial inoculum of one kind and its preparation method and application
CN104609541B (en) The direct biochemical processing method of a kind of decanedioic acid not desalination of waste water
CN111548952A (en) Method for domesticating microbial flora for degrading efficient sulfur-series malodorous substances
CN110551665A (en) method for culturing tobacco sewage flora
CN110182948A (en) Biological method for treating waste water based on activated sludge fermentation material
Dong et al. Application of microbial technology in waste water treatment
CN114292782B (en) Bacterium for strengthening facultative FMBR (anaerobic fermentation and fermentation) pesticide wastewater treatment process
CN115340194B (en) Method for cooperatively removing hexavalent chromium by sludge iron-rich biochar and pseudomonas aeruginosa

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20201127

WD01 Invention patent application deemed withdrawn after publication