CN113351641A - Method for remedying heavy metal pollution of smelting slag through non-covering organisms - Google Patents
Method for remedying heavy metal pollution of smelting slag through non-covering organisms Download PDFInfo
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Abstract
The invention discloses a method for remedying heavy metal pollution of smelting slag by covering-free organisms. Belongs to the technical field of environmental protection. The method comprises the five steps of respectively preparing aerobic engineering bacteria liquid and anaerobic engineering bacteria liquid, preparing smelting slag heavy metal solidified bacteria liquid, preparing smelting slag piles, spraying heavy metal solidified bacteria liquid and curing and solidifying. By utilizing the characteristics of aerobic microorganisms and anaerobic microorganisms, a selective smelting slag heavy metal pollution coverage-free bioremediation system is constructed, so that the bioavailability of heavy metal in the selective smelting slag is reduced by 80-95%, the repair construction difficulty of the selective smelting slag heavy metal pollution is reduced, and the heavy metal solidification/stabilization effect is improved.
Description
Technical Field
The invention relates to the technical field of environmental protection, in particular to a method for remedying heavy metal pollution of smelting slag by uncovered organisms.
Background
The non-ferrous metal industry generates a large amount of solid wastes such as mining waste rocks, mineral processing slag, smelting slag and the like, and only 2019 years produces 4.6 million tons of tailings, 3132.3 million tons of smelting slag and 7445.8 million tons of slag (including ferrous metals). The ecological environment around the mine is deteriorated due to the large amount of stockpiled smelting slag. Therefore, how to effectively control the heavy metals in the smelting slag becomes the key point and the difficulty of the environmental pollution treatment of the mine.
The soil heavy metal pollution remediation technology comprises three major categories of physical remediation, chemical remediation and biological remediation. The bioremediation has the advantages of high efficiency and environmental protection, is easy to be accepted by the public, and becomes a hotspot of the current research.
Under anaerobic conditions, Sulfate Reducing Bacteria (SRB) reduce SO by dissimilatory sulfate4 2-Reduction to H2S, heavy metal ion and H2And (3) under the action of S, heavy metal sulfide precipitate with small solubility product constant is generated, so that the biological effectiveness of the heavy metal is reduced.
Schwertmannite is a metastable secondary hydroxyl ferric sulfate mineral with poor crystallinity and special appearance, which is discovered by Bigham et al (1990) when the characteristics of acid mine wastewater precipitates are researched, and the theoretical chemical formula of the metastable secondary hydroxyl ferric sulfate mineral can be expressed as Fe8O8(OH)6SO4And the sulfate is commonly present in an acidic environment with rich sulfate (such as acidic mine wastewater and acidic sulfate soil).
Schlerian minerals and jarosite are formed, and are closely related to the oxidation of aerobic microorganisms such as Acidithiobacillus ferrooxidans. The acidophilic thiobacillus ferrooxidans is a medium-temperature, aerobic, acidophilic and obligate inorganic chemoautotrophic bacterium, and removes heavy metals in acid mine wastewater by participating in mineralization, precipitation and co-adsorption in the process of inducing the formation of Schwerer minerals.
The aerobic microorganism or the anaerobic microorganism is independently used for solidifying/stabilizing the heavy metal in the smelting slag, certain defects exist, the aerobic microorganism is suitable for living in the surface layer oxidation environment of the smelting slag pile, and induces the surface layer heavy metal to participate in the formation, precipitation and precipitation of schleman mineralsThe functions of co-adsorption and the like are stabilized, while the activity of aerobic microorganisms in the anoxic environment of the middle and lower layers is low, the solidification effect on heavy metals in the smelting slag is poor, and even no solidification effect exists at all; anaerobic microorganisms are suitable for living in the lower layer reduction environment in the dressing and smelting slag pile, and SO is converted by using dissimilation4 2-Reduction to H2S, vulcanizing and curing heavy metals, wherein in a surface oxidation environment, the activity of anaerobic microorganisms is extremely low, the curing effect on the surface heavy metals of the dressing and smelting slag pile is poor, and the heavy metals in the curing/stabilizing dressing and smelting slag are large in engineering quantity, high in construction cost and the like.
Therefore, how to provide a method for remedying the heavy metal pollution of the smelting slag by uncovered organisms is a problem which needs to be solved urgently by the technical personnel in the field.
Disclosure of Invention
In view of the above, the invention provides a method for remedying heavy metal pollution of smelting slag by covering-free organisms.
Compared with the conventional method for solidifying the heavy metal in the smelting slag, the method has the following characteristics: firstly, aerobic microorganisms solidify/stabilize surface layer heavy metal in an oxidation environment, and anaerobic microorganisms solidify/stabilize middle and lower layer heavy metal in a reduction environment; selecting and smelting the surface layer of the slag pile, growing and breeding aerobic microorganisms, consuming oxygen in the slag pile, blocking oxygen in the air from migrating to the lower layer of the slag pile, and creating an anaerobic environment for the middle and lower layers; the middle and lower layers of the slag pile are in anaerobic environment, so that the growth and the propagation of anaerobic microorganisms are facilitated, and heavy metals are solidified/stabilized in the form of metal sulfides and are not easy to dissolve secondarily; and fourthly, stabilizing heavy metals on the surface layer of the dressing and smelting slag pile by participating in the formation, precipitation, co-adsorption and the like of schneiderian minerals.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for remedying heavy metal pollution of smelting slag by covering-free organisms sequentially comprises the following steps:
(1) respectively preparing aerobic engineering bacteria liquid and anaerobic engineering bacteria liquid;
(2) preparing a heavy metal solidifying bacterial liquid of the smelting slag;
(3) preparing a smelting slag pile, laying two layers of agricultural and forestry wastes with the thickness of 0.2-0.3 m at proper positions in the smelting slag pile;
(4) spraying heavy metal solidified bacteria liquid: spraying heavy metal solidified bacteria liquid of anaerobic engineering bacteria on the surface of the slag selecting and smelting pile, wherein the spraying strength is 2.0-15.0L/m2H, after the time interval is 1.0d to 10.0d, spraying aerobic engineering bacteria heavy metal solidified bacteria liquid on the surface of the slag pile, wherein the spraying strength is 1.0L/m to 10.0L/m2·h；
(5) And (5) curing and solidifying.
Further: adjusting the laying position according to the parameters of the selected and smelted slag granularity, the slag pile height, the permeability and the like; further, the method comprises the following steps: in the dressing-smelting slag pile, the distance between the dressing-smelting slag pile and the surface is between 0.8 and 1.20 meters and between 2.0 and 2.40 meters.
Preferably: the aerobic engineering bacterial liquid in the step (1) comprises the following steps: mixing one or more of Acidithiobacillus ferrooxidans liquid, Acidithiobacillus thiooxidans liquid, Acidithiobacillus ferrooxidans liquid, and Acidithiobacillus mesophilic liquid, and performing amplification culture by using sterilized 9K culture medium; wherein, when several kinds are mixed, the volume ratio of every two kinds of bacterium liquid is 3: 1-1: 3.
has the advantages that: the bacteria synergistic effect can improve the conversion rate of the Schwerer mineral.
Preferably: the culture temperature of the amplification culture is 10-35 ℃, and the amplification culture is carried out until the content of each bacterium is 3.0 multiplied by 106～5.0×1010cfu/ml。
Has the advantages that: aerobic engineering bacteria liquid capable of oxidizing Fe2+Is Fe3+Chemical energy is obtained, and schlerian minerals, jarosites (jarosites, ammoniojarosites, jarosites, etc.) and the like are associated.
Preferably: step (1), anaerobic engineering bacteria liquid: the sulfate reducing bacteria are obtained after enlarged culture, and the adopted culture medium comprises the following raw materials: k2HPO40.1～1.0g/L，Na2SO40.5～1.5g/L，CaCl2·2H2O 1.5～2.5g/L，MgSO4·7H21.5-2.5 g/L of O, 1.5-2.5 g/L of sodium lactate with the purity of 60%, and 0.5-1.5 g/L of yeast extract.
Has the advantages that: increasing the biomass and activity of the sulfate reducing bacteria.
Preferably: conditions for the scale-up culture of sulfate-reducing bacteria: after inoculation, aerating nitrogen, and then placing the mixture in a constant-temperature incubator for sealed culture, wherein the culture temperature is 10-35 ℃.
Preferably: the step (2) of selecting and smelting heavy metal solidified bacterial liquid of slag comprises the following steps: aerobic engineering bacteria heavy metal solidified bacteria liquid and anaerobic engineering bacteria heavy metal solidified bacteria liquid.
Preferably: the aerobic engineering bacterium heavy metal solidified bacterium liquid comprises: (NH)4)2SO40.05～3.50g/L，MgSO4·7H2O is 0.05-0.35 g/L, the pH value is adjusted to 1.0-7.0 before inoculation, and the inoculation amount of aerobic engineering bacteria liquid is 5-15% by volume ratio.
Preferably: the anaerobic engineering bacterium heavy metal solidified bacterium liquid comprises: na (Na)2SO40.2-1.5 g/L, adjusting the pH value to 5.0-10.0 before inoculation, and the inoculation amount of anaerobic engineering bacteria is 5-15% by volume ratio.
Preferably: the agricultural and forestry wastes in the step (3) comprise one or more of corncobs, straws, rice straws, bran coats and rice husks, and the weight ratio of any two is 1: 1.
has the advantages that: the smelting slag and the elements or organic matters in the agricultural and forestry solid waste are used as nutrients, so that the engineering application cost is reduced.
Preferably: curing and solidifying: curing for 30-180 days at 5.0-40 ℃.
According to the technical scheme, compared with the prior art, the invention provides the method for remedying the heavy metal pollution of the smelting slag by the uncovered organisms, the aerobic microorganisms in the oxidizing environment of the slag pile are easy to grow and breed, the aerobic microorganisms induce the heavy metal on the surface of the smelting slag pile to be solidified/stabilized by participating in the formation, sedimentation, co-adsorption and the like of schlerz minerals, the aerobic microorganisms consume oxygen in the slag pile, the air is prevented from moving downwards, the anaerobic environment is created for the middle and lower layers, the anoxic environment of the middle and lower layers is suitable for the growth and breeding of the anaerobic microorganisms, and the heavy metal is vulcanized and solidified/stabilized, so that the ecological system for remedying the heavy metal in the smelting slag by the uncovered organisms is formed, and after natural maintenance at normal temperature, the biological effectiveness of the heavy metal in the smelting slag can be reduced by 80-95%.
Detailed Description
The following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention discloses a method for remedying heavy metal pollution of smelting slag by uncovered organisms.
The starting materials and microorganisms mentioned in the examples are commercially available and are not described in detail here.
Example 1
A method for remedying heavy metal pollution of smelting slag by covering-free organisms sequentially comprises the following steps:
(1) respectively preparing aerobic engineering bacteria liquid and anaerobic engineering bacteria liquid;
(2) preparing a heavy metal solidifying bacterial liquid of the smelting slag;
(3) preparing a smelting slag pile, and paving two layers of agricultural and forestry wastes at proper positions in the smelting slag pile;
(4) spraying heavy metal solidified bacteria liquid:
(5) and (5) curing and solidifying.
In order to further optimize the technical scheme: the aerobic engineering bacterial liquid comprises: the bacillus thiobacillus ferrooxidans leptospira acidophilus and the thiobacillus mesophilic bacteria liquid have the volume ratio of 3:3: and 1, adopting a sterilized 9K culture medium for amplification culture.
In order to further optimize the technical scheme: the culture temperature of the scale-up culture is 10 ℃, and the culture is carried out until the content of each bacterium is 3.0 multiplied by 106cfu/ml。
In order to further optimize the technical scheme: step (1), anaerobic engineering bacteria liquid: the sulfate reducing bacteria are obtained after enlarged culture, and the adopted culture medium comprises the following raw materials: k2HPO40.1 g/L，Na2SO41.5 g/L，CaCl2·2H2O 1.5g/L，MgSO4·7H2O 2.5g/L, 1.5g/L sodium lactate with the purity of 60 percent and 1.5g/L yeast extract.
In order to further optimize the technical scheme: conditions for the scale-up culture of sulfate-reducing bacteria: after inoculation, nitrogen is aerated and then the mixture is placed in a constant temperature incubator for sealed culture, and the culture temperature is 10 ℃.
In order to further optimize the technical scheme: the step (2) of selecting and smelting heavy metal solidified bacterial liquid of slag comprises the following steps: aerobic engineering bacteria heavy metal solidified bacteria liquid and anaerobic engineering bacteria heavy metal solidified bacteria liquid.
In order to further optimize the technical scheme: the aerobic engineering bacterium heavy metal solidified bacterium liquid comprises: (NH)4)2SO4 0.05g/L，MgSO4·7H2O0.05 g/L, the pH value is adjusted to 1.0 before inoculation, and the inoculation amount of aerobic engineering bacteria liquid is 5 percent by volume ratio.
In order to further optimize the technical scheme: the anaerobic engineering bacterium heavy metal solidified bacterium liquid comprises: na (Na)2SO40.2g/L, adjusting the pH value to 5.0 before inoculation, and the inoculation amount of anaerobic engineering bacteria is 5 percent by volume ratio.
Example 2
A method for remedying heavy metal pollution of smelting slag by covering-free organisms sequentially comprises the following steps:
(1) respectively preparing aerobic engineering bacteria liquid and anaerobic engineering bacteria liquid;
(2) preparing a heavy metal solidifying bacterial liquid of the smelting slag;
(3) preparing a smelting slag pile, and paving two layers of agricultural and forestry wastes at proper positions in the smelting slag pile;
(4) spraying heavy metal solidified bacteria liquid:
(5) and (5) curing and solidifying.
In order to further optimize the technical scheme: the aerobic engineering bacterial liquid comprises: carrying out amplification culture on the acidophilic thiobacillus thiooxidans bacterial liquid, the thiobacillus thiooxidans bacterial liquid and the ferrous oxide leptospirillum bacterial liquid by adopting a sterilized 9K culture medium according to the volume ratio of 3:3: 1.
In order to further optimize the technical scheme: the culture temperature of the amplification culture is 25 ℃, and the amplification culture is carried out until the content of each bacterium is 4.0 multiplied by 108cfu/ml。
In order to further optimize the technical scheme: step (ii) of(1) Anaerobic engineering bacteria liquid: the sulfate reducing bacteria are obtained after enlarged culture, and the adopted culture medium comprises the following raw materials: k2HPO40.5 g/L，Na2SO41 g/L，CaCl2·2H2O 2.0g/L，MgSO4·7H2O2.0 g/L, sodium lactate with the purity of 60 percent 2.0g/L and yeast extract 1.0 g/L.
In order to further optimize the technical scheme: conditions for the scale-up culture of sulfate-reducing bacteria: after inoculation, nitrogen is aerated and then the mixture is placed in a constant temperature incubator for sealed culture, and the culture temperature is 25 ℃.
In order to further optimize the technical scheme: the step (2) of selecting and smelting heavy metal solidified bacterial liquid of slag comprises the following steps: aerobic engineering bacteria heavy metal solidified bacteria liquid and anaerobic engineering bacteria heavy metal solidified bacteria liquid.
In order to further optimize the technical scheme: the aerobic engineering bacterium heavy metal solidified bacterium liquid comprises: (NH)4)2SO4 2.50g/L，MgSO4·7H2O0.15 g/L, the pH value is adjusted to 4.0 before inoculation, and the inoculation amount of aerobic engineering bacteria liquid is 10 percent by volume ratio.
In order to further optimize the technical scheme: the anaerobic engineering bacterium heavy metal solidified bacterium liquid comprises: na (Na)2SO40.2g/L, adjusting the pH value to 5.0 before inoculation, and the inoculation amount of anaerobic engineering bacteria is 5 percent by volume ratio.
Example 3
A method for remedying heavy metal pollution of smelting slag by covering-free organisms sequentially comprises the following steps:
(1) respectively preparing aerobic engineering bacteria liquid and anaerobic engineering bacteria liquid;
(2) preparing a heavy metal solidifying bacterial liquid of the smelting slag;
(3) preparing a smelting slag pile, and paving two layers of agricultural and forestry wastes at proper positions in the smelting slag pile;
(4) spraying heavy metal curing bacteria liquid;
(5) and (5) curing and solidifying.
In order to further optimize the technical scheme: the aerobic engineering bacterial liquid comprises: the method comprises the following steps of carrying out amplification culture on a sterilized 9K culture medium by using a mesophilic thiobacillus thiooxidans liquid, a leptospirillum ferrooxidans liquid and a mesophilic thiobacillus liquid in a volume ratio of 3:3:1: 1.
In order to further optimize the technical scheme: the culture temperature of the amplification culture is 35 ℃, and the amplification culture is carried out until the content of each bacterium is 5.0 multiplied by 1010cfu/ml。
In order to further optimize the technical scheme: step (1), anaerobic engineering bacteria liquid: the sulfate reducing bacteria are obtained after enlarged culture, and the adopted culture medium comprises the following raw materials: k2HPO41.0 g/L，Na2SO41.5 g/L，CaCl2·2H2O 2.5g/L，MgSO4·7H2O1.5 g/L, sodium lactate with the purity of 60 percent 2.5g/L and yeast extract 0.5 g/L.
In order to further optimize the technical scheme: conditions for the scale-up culture of sulfate-reducing bacteria: after inoculation, nitrogen is aerated and then the mixture is placed in a constant temperature incubator for sealed culture, and the culture temperature is 35 ℃.
In order to further optimize the technical scheme: the step (2) of selecting and smelting heavy metal solidified bacterial liquid of slag comprises the following steps: aerobic engineering bacteria heavy metal solidified bacteria liquid and anaerobic engineering bacteria heavy metal solidified bacteria liquid.
In order to further optimize the technical scheme: the aerobic engineering bacterium heavy metal solidified bacterium liquid comprises: (NH)4)2SO4 3.50g/L，MgSO4·7H2O0.35 g/L, the pH value is adjusted to 7.0 before inoculation, and the inoculation amount of aerobic engineering bacteria liquid is 15 percent by volume ratio.
In order to further optimize the technical scheme: the anaerobic engineering bacterium heavy metal solidified bacterium liquid comprises: na (Na)2SO41.5g/L, adjusting the pH value to 10.0 before inoculation, and controlling the inoculation amount of anaerobic engineering bacteria to be 15 percent according to the volume ratio.
The experimental effect is as follows:
(1) the slag amount of a certain copper ore dressing and smelting slag is about 15000m3Minerals such as fayalite, biotite, lead silicate, quartz, olivine, pyroxene, sphalerite, chalcopyrite, magnetite pyrite, limonite, ferrochromite, albite, chlorite and the like. The toxicity leaching test result shows that the main overproof elements are Cu, Pb, Zn and the like.
The height of the slag pile for dressing and smelting is 12.5m, the maximum granularity is less than 1.0mm, and the permeability coefficient is 1.5 multiplied by 10-4cm/s inPaving corn cobs and straws with the thickness of 0.2m between 1.0m and 1.20m and between 2.20m and 2.40m away from the surface of the slag heap, wherein the weight ratio of the corn cobs to the straws is 1: 1.
the heavy metal curing liquid prepared in the example is distributed by adopting a dripping method, firstly, the heavy metal curing liquid with the inoculum size of 10 percent of anaerobic microorganisms is distributed, and the dripping intensity is 3.0L/m2H, after 5d, spraying heavy metal solidified bacterial liquid with 10 percent (volume ratio) of aerobic microorganism with dripping intensity of 1.0L/m2H, after natural curing for 100 days at normal temperature (20-35 ℃), the biological effectiveness of Cu, Pb and Zn in the smelting slag is reduced by 90.12%, 95.56% and 89.66% in sequence.
(2) The slag amount of a certain nickel smelting slag is about 10000m3The mineral composition contains minerals such as olivine, quartz, biotite, chlorite, potash feldspar, albite, limonite, kaolinite, ilmenite, apatite, manganite, rutile, pyrite, magnetite, anorthite, ferric phosphate, sphene, monazite, chalcopyrite, bastnaesite, zircon, dolomite, chalcocite, diopside and the like. The toxicity leaching test result shows that the main overproof elements are Cu, Ni and the like.
The height of the slag pile for dressing and smelting is 10.5m, the maximum granularity is less than 1.0mm, and the permeability coefficient is 5.5 multiplied by 10-4And cm/s, paving 0.3m thick mixture of corncobs, straws, rice straws, bran coats and rice hulls between 0.70m and 1.00m and between 2.0m and 2.30m away from the surface of the slag pile, wherein the weight ratio of the corncobs, the straws, the bran coats and the rice hulls is 1: 1.
the heavy metal curing liquid prepared in the example is sprayed by adopting a spraying method, firstly, the heavy metal curing liquid of 5.0% anaerobic microorganism is sprayed, and the spraying strength is 5.0L/m2H, after 5d, spraying heavy metal solidified bacterial liquid with 10% (volume ratio) aerobic microorganisms, wherein the spraying strength is 3.0L/m2H, after natural curing for 150 days at normal temperature (15-35 ℃), the biological effectiveness of Cu and Ni in the smelting slag is reduced by 91.32% and 93.04% in sequence.
(3) The slag amount of certain lead-zinc smelting slag is about 12000m3Mainly contains FeO and SiO2、Al2O3CaO, MgO, ZnO, etc., in the form of compound, solid solution, eutectic crystalMixtures, and the like, as well as sulfides, fluorides, and the like. The toxicity leaching test result shows that the main overproof elements are Pb, Zn, Cr, Cd and the like.
The height of the slag pile for dressing and smelting is 13.5m, the maximum granularity is less than 1.0mm, and the permeability coefficient is 5.4 multiplied by 10-4And cm/s, paving a mixture of straw, bran and rice hull with the thickness of 0.3m between 0.70m and 1.00m and between 2.0m and 2.30m away from the surface of the slag pile, wherein the weight ratio of the straw to the bran to the rice hull is 1: 1.
the heavy metal curing liquid prepared in the example is sprayed by adopting a spraying method, firstly, the heavy metal curing liquid of anaerobic microorganism with the inoculation amount of 5.0 percent is sprayed, and the spraying strength is 5.5L/m2H, after 6d, spraying heavy metal solidified bacterial liquid with 10% (volume ratio) aerobic microorganisms, wherein the spraying strength is 3.0L/m2H, after natural curing for 190 days at normal temperature (15-35 ℃), the biological effectiveness of Pb, Zn, Cr and Cd in the smelting slag is reduced by 92.22%, 89.16%, 90.21% and 91.34% in sequence.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A method for remedying heavy metal pollution of smelting slag by covering-free organisms is characterized by sequentially comprising the following steps:
(1) respectively preparing aerobic engineering bacteria liquid and anaerobic engineering bacteria liquid;
(2) preparing a heavy metal solidifying bacterial liquid of the smelting slag;
(3) preparing a smelting slag pile, laying two layers of agricultural and forestry wastes with the thickness of 0.2-0.3 m at proper positions in the smelting slag pile;
(4) spraying heavy metal solidified bacteria liquid: spraying heavy metal solidified bacteria liquid of anaerobic engineering bacteria on the surface of the slag selecting and smelting pile, wherein the spraying strength is 2.0-15.0L/m2H, after the time interval is 1.0d to 10.0d, spraying aerobic engineering bacteria heavy metal solidified bacteria liquid on the surface of the slag pile, wherein the spraying strength is 1.0L/m to 10.0L/m2·h；
(5) And (5) curing and solidifying.
2. The method for remediating the heavy metal pollution of the smelting slag through the uncovered organisms according to the claim 1, wherein the aerobic engineering bacteria liquid in the step (1) comprises the following steps: mixing one or more of Acidithiobacillus ferrooxidans liquid, Acidithiobacillus thiooxidans liquid, Acidithiobacillus ferrooxidans liquid, and Acidithiobacillus mesophilic liquid, and performing amplification culture by using sterilized 9K culture medium; wherein, when several kinds are mixed, the volume ratio of every two kinds of bacterium liquid is 3: 1-1: 3.
3. the method of claim 2, wherein the culture temperature of the expanded culture is 10-35 ℃, and the culture is carried out until the bacteria content is 3.0 x 106～5.0×1010cfu/ml。
4. The method for the non-covering bioremediation of the heavy metal pollution of the smelting slag according to claim 1, wherein the step (1) of anaerobic engineering bacteria liquid: the sulfate reducing bacteria are obtained after enlarged culture, and the adopted culture medium comprises the following raw materials: k2HPO4 0.1～1.0g/L，Na2SO4 0.5～1.5g/L，CaCl2·2H2O 1.5～2.5g/L，MgSO4·7H21.5-2.5 g/L of O, 1.5-2.5 g/L of sodium lactate with the purity of 60%, and 0.5-1.5 g/L of yeast extract.
5. The method for the non-covering bioremediation of the heavy metal pollution of smelting slag according to claim 4, wherein the conditions of the sulfate-reducing bacteria are: after inoculation, aerating nitrogen, and then placing the mixture in a constant-temperature incubator for sealed culture, wherein the culture temperature is 10-35 ℃.
6. The method for remediating the heavy metal pollution of the smelting slag through the uncovered organisms according to the claim 1, wherein the heavy metal solidified bacterial liquid of the smelting slag in the step (2) comprises: aerobic engineering bacteria heavy metal solidified bacteria liquid and anaerobic engineering bacteria heavy metal solidified bacteria liquid.
7. The method for the uncovered bioremediation of the heavy metal pollution of the smelting slag according to claim 6, wherein the aerobic engineering bacteria heavy metal solidified bacterial liquid comprises: (NH)4)2SO4 0.05～3.50g/L，MgSO4·7H2O is 0.05-0.35 g/L, the pH value is adjusted to 1.0-7.0 before inoculation, and the inoculation amount of aerobic engineering bacteria liquid is 5-15% by volume ratio.
8. The method of claim 6, wherein the anaerobic engineering bacteria heavy metal solidified bacterial liquid comprises: na (Na)2SO40.2-1.5 g/L, adjusting the pH value to 5.0-10.0 before inoculation, and the inoculation amount of anaerobic engineering bacteria is 5-15% by volume ratio.
9. The method for the non-covering bioremediation of the dressing and smelting slag contaminated with heavy metals according to claim 1, wherein the forestry and agricultural residues in step (3) include one or more of corncobs, stalks, straws, bran coats and rice husks, and the weight ratio of any two of them is 1: 1.
10. the method for the non-covering bioremediation of the heavy metal pollution of sorted smelting slag according to claim 1, characterized in that the curing and solidification of step (5): curing for 30-180 days at 5.0-40 ℃.
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| CN114951245A (en) * | 2022-05-27 | 2022-08-30 | 广东桃林生态环境有限公司 | Method for preventing surface water of heavy metal mining waste land from seeping downwards and application of method in treatment of heavy metal mining waste land |
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