CN108856283A - A kind of repairing method of microorganism in the place containing heavy metal waste slag stockpiling - Google Patents
A kind of repairing method of microorganism in the place containing heavy metal waste slag stockpiling Download PDFInfo
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- CN108856283A CN108856283A CN201810582925.XA CN201810582925A CN108856283A CN 108856283 A CN108856283 A CN 108856283A CN 201810582925 A CN201810582925 A CN 201810582925A CN 108856283 A CN108856283 A CN 108856283A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
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Abstract
Disclosed by the invention is the method for the microorganism remediation in the place containing heavy metal waste slag stockpiling, by using the hazardous waste of the heavy metals such as leaded, zinc, cadmium as culture medium, sulfate reducing bacteria is tamed, sulfate reducing bacteria survival ability and environmental suitability in the hazardous waste of the heavy metals such as lead, zinc, cadmium, arsenic are improved；It adds all kinds of nutriments and mixes sulfate reducing bacteria with bacillus subtilis and strengthened to the reparation of soil, remove polluter rapidly, restore soil crop planting ability.
Description
Technical field
The present invention relates to a kind of heavy metal waste slag stockpiling place and the restorative procedures of Soil Surrounding pollution, belong to environmental protection
Field.
Background technique
The pollution amelioration for storing up place and periphery containing heavy metal waste slags such as cadmium, lead, zinc need to be paid attention to.Due to some dangerous wastes
Object production and disposition, the enterprise pollution precautionary measures are not in place, cause these typical hazard wastes in long-term stockpiling and transfer process
Middle generation is leaked and is scattered, and causes place and Soil Surrounding to be contaminated, or even cause the pollution of underground water, and uses tradition side
Formula reparation contaminated site is costly, and general enterprises can not undertake, therefore contaminated site, by long-term existence, pollutant is with precipitation
It drenches with rain lasting diffusion, greater area of pollution is caused to environment, or even threaten the health of the people.Therefore, on exploiting economy
It is feasible, technical reliable site remediation technology, to prevent the lasting diffusion of Hazardous Wastes Management that there is strongly urgent
Property.
The disposal options of the heavy metal pollution caused by hazardous waste mainly have physical treatment, chemical treatment, biological treatment.
Not only cost is very high for the repair mode of physics and chemistry, but also the property of malleable soil, causes the consequence of the plant underproduction, unfavorable
It in sustainable development, therefore is not ideal processing method.The biological prosthetic main packet of hazardous waste stockpiling place heavy metal pollution
Include microorganism remediation and phytoremediation.Phytoremediation is also current more promising heavy metal pollution recovery technique, but plant is repaired
Multiple difficulty is：1. the electrochemical conditions and heavy metal toxin due to being not suitable for plant life cause plant survival rate low, stock
Time is short, it is difficult to establish sustainable plant ecosystem.2. slow heavy metals removal speed and incomplete heavy metal generation
It thanks and limits the application of phytoremediation.It should also be emphasized that the above method is just for contaminated by heavy metals place
(without new source of heavy metal pollution), it is difficult to which applied to the contaminated site that heavy metal continually dissolves out is administered, (hazardous waste is stored up
Place).And microorganism remediation is not only suitable in contaminated by heavy metals soil remediation, and it is constantly molten to be also adapted for heavy metal ion
The reparation in place out, but also have at low cost, repairing effect is good, destroys to environment and soil texture small, and it is extensive to be conducive to ecology
The features such as multiple, therefore in comparison microorganism remediation has bigger development space and application prospect.
Currently with microorganism carry out heavy metal pollution microorganism remediation at home, it is outer have backup attempt.Processing is former
Reason：
H2S+M2+→MS↓+2H+
Wherein M is the metal cations such as Fe, Ni, Cu, Cd, Cr, Zn.
The microorganism remediation mode of heavy metal pollution is mainly that certain micro-organisms has sulfate reduction ability, can be incited somebody to action
It is reduced into S2-, reaction product S2-It reacts to form precipitating with the heavy metal ion being dissolved out in mine, thus by solubility weight
Metal immobilization and stabilisation.Sulfate reducing bacteria processing heavy metal method processing cost is low, and processing heavy metal type is more, is applicable in
Property is strong, without secondary pollution.
Most of bacillus belong to gram-positive bacteria, are that can also generate gemma in shortage nutrition and poor environment.Perhaps
More research discovery bacillus are obvious to the adsorption effect of heavy metal ion, and have to heavy metals such as Cd, Pb, Cr stronger anti-
Property, while containing a large amount of peptide glycans and teichoic acid in B. subtilis cell wall, therefore a large amount of carboxyl can be provided, amide groups
Isoreactivity group, these groups can lose proton and make phage surface with stronger negative electrical charge, make it to inhale by electrostatic attraction
Attached heavy metal ion.
Summary of the invention
The hazardous waste of the heavy metals such as present invention lead, zinc, cadmium, arsenic carries out sulfate reducing bacteria as culture medium
Domestication improves survival ability and environmental suitability of the sulfate reducing bacteria in the hazardous waste of the heavy metals such as lead, zinc, cadmium, arsenic,
The large-scale culture for optimizing sulfate reducing bacteria carries out the hazardous waste heap of the heavy metals such as fairly large leaded, zinc, cadmium, arsenic
It deposits place heavy metal pollution original position and Soil Surrounding is repaired.
The purpose of the present invention is what is be achieved through the following technical solutions：
The repairing method of microorganism in the place containing heavy metal waste slag stockpiling, as medium, to be filtered out suitable containing heavy metal waste slag
Answer the sulfate reducing bacteria survived in the hazardous waste soil of heavy metal, be added by anaerobic lactic acid fermentation sweet sorghum stalk and
The culture medium that nutrients mixes adjusts culture medium PH 6-8, then remixes bacillus subtilis and carries out heavy metal waste slag
Place soil remediation is stored up, soil reaches normal crop planting nutritional need, includes the following steps：
(1) strain acquires：It is acquired from nonferrous metals in Guangxi Mineral Processing Enterprises Tailings Dam soil；
(2) it ferments：By sweet sorghum stalk crushing be placed in it is closed in fermentor, carried out under room temperature lactic fermentation 8-12 days it is standby
With；
(3) prepared by culture medium：Other nutritional ingredients are added into sweet sorghum stalk fermentation liquid, culture medium is made, adjusts culture
Base PH 6-8；
(4) medium sterilization：Culture medium is placed in Anaerobic culturel bottle mesohigh steam sterilizing, the pressure 101 that sterilizes~
105kPa, 118-123 DEG C of temperature, sterilization time 15-25min；
(5) screening of sulfate reducing bacteria：It is proportionally added into the soil containing heavy metal waste slag in the medium, then is cultivating
Collected strain in 0.3~0.6mL/g step (1) is added in base, visible black color precipitates after culture 3-4 days, illustrates that strain is raw
It is long good, then filter out good sulfate reducing bacteria；
(6) sulfate reducing bacteria expands culture：Under aseptic technique, step (5) are added into sterilized culture medium
Sulfate reducing bacteria 0.1~the 0.5mL/g of bacterium solution screened, sealing and standing expand culture 3-5 days；
(7) by bacillus subtilis microbial agent and water according to mass ratio 1:500~800 are made into bacillus subtilis bacterium solution, withered
Careless bacillus bacterium solution and the sulfate reducing bacteria for expanding culture carry out being mixed and made into mixed bacteria according to a certain percentage；
(8) remediating heavy metal soil：At normal temperature, culture medium solution pH is 6-7 after regulating step (4) sterilizing, then
By culture medium solution after mixed bacteria liquid and sterilizing according to 1：10~12 access mix, to heavy metal waste slag stockpiling place soil according to
0.6~0.8L/m2Amount sprinkling access mixed bacteria culture medium solution, then carry out heavy metal waste slag stockpiling place soil repair
It is multiple；
(9) concentration of heavy metal ion is measured：Measure concentration of heavy metal ion：It is stored up every 5-7 days measurement heavy metal waste slags
PH and lead, zinc, cadmium metal ion concentration in the soil solution of place, and continue to add culture medium solution after step (4) sterilize
0.6~0.8L/m2, after repairing 20-28 days, soil, which seeps concentration of heavy metal ion in filtrate, can reach GB25466-2010《Lead,
Zn Cr coating pollutant emission standard》Standard.
As a preferred option of the technical scheme, step (2) the sweet sorghum stalk powder and lactic acid bacteria proportionally 200~300:1
Mixing and water adding stirs evenly, and makes water tariff collection 30~50%, sealed fermenting.
As a preferred option of the technical scheme, the nutritional ingredient of step (3) addition is：0.4~0.6g/L of epsom salt,
0.9~1.2g/L of ammonium sulfate, 0.4~0.6g/L of potassium dihydrogen phosphate, 0.4~0.6g/L of ferrous sulfate heptahydrate, multifunction nutritive is not
Only it is suitble to the growth of bacillus subtilis conducive to the growth-promoting media of sulfate reducing bacteria.
As a preferred option of the technical scheme, the step (3) adjusts medium pH using sodium hydroxide is slowly intermittent, so that
Sodium hydroxide can sufficiently react to obtain sodium lactate with the lactic acid of fermentation.
As a preferred option of the technical scheme, the soil of heavy metal waste slag and the mass ratio of culture medium is added in the step (5)
It is 2~3:1, nutritional ingredient obtains maximum benefit in culture medium while effectively removing heavy metal ions in soil under the ratio
With change.
As a preferred option of the technical scheme, step (7) mixed bacteria is bacillus subtilis bacterium solution and sulfate reduction
Bacterium is according to mass ratio 5~10:1 is mixed.
As a preferred option of the technical scheme, step (8) initial pH value is adjusted using sodium hydroxide solution.
As a preferred option of the technical scheme, the step (9) is described uses a huge sum of money in AFS DETERMINATION leachate
Belong to element species and content, atomic fluorescence spectrometry has very high sensitivity, and the range of linearity of calibration curve is wide, can be carried out more
Element measures simultaneously.
Above-mentioned bacillus subtilis microbial agent can be commercially available on the market.
Beneficial effects of the present invention：
1. the place soil that the heavy metal pollution and heavy metal repaired through the invention continually dissolve out, compared to plant
Object reparation is quick, and effect is more preferable, at low cost, certain serious contaminated soils of heavy metal, and traditional phytoremediation can not reality
Existing, plant is difficult to survive, and the pollution that the method for the present invention stores up place and periphery to heavy metal waste slag is repaired, in soil
Leachate heavy metal ion content is lower than requirement limit value of the national requirements normal discharge containing heavy metal contaminants.
2. the present invention, which is mixed using sulfate reducing bacteria with bacillus subtilis, carries out counterweight metal waste residue stockpiling place
Microorganism remediation, sulfate reducing bacteria has synergistic effect to bacillus subtilis during heavy metal in rehabilitating soil, withered
Careless bacillus is more preferable compared with exclusive use effect to the adsorption capacity effect of heavy metal in soil, corresponding sulfate reducing bacteria
The heavy metal of bacillus subtilis absorption is handled, compared to exclusive use sulfate reducing bacteria to the soil of heavy metal pollution
Earth remediation efficiency is higher, and effect is more preferable.
3. using primary carbon source of the sweet sorghum stalk as sulfate reducing bacteria, pH value is adjusted by using sodium hydroxide,
So that sodium hydroxide reacts to obtain sodium lactate with the lactic acid that sweet sorghum stalk ferments, the reduction of sulfate reducing bacteria is more conducively improved
Rate, while also can be as the nutrient source of bacillus subtilis, not only raw material is cheap and easily-available, also as save cost.
4. the present invention directly repairs contaminated soil, meanwhile, also solve the underground due to heavy metal pollution
The soil that the source of water was repaired can carry out vegetation or crop-planting, bacillus subtilis soil nutrition diversification and change
The comprehensive treatment to land resource environment, built environment friendly society are realized during kind soil.
Specific embodiment
Below with reference to embodiment, the present invention is further described.
Embodiment 1
A. acidophilus strain and sulfate reduction strain are acquired from nonferrous metals in Guangxi Mineral Processing Enterprises Tailings Dam soil；
B. sweet sorghum stalk crushing is placed in fermentor, with lactic acid bacteria proportionally 200:1 mixing, adds water and stirs
It is even, make water tariff collection 50%, sealed fermenting spontaneous fermentation 3 days are spare；
C. after fermentation, sweet sorghum stalk fermentation liquid is taken out, epsom salt 0.4g/L, ammonium sulfate 1.2g/ is added
L, potassium dihydrogen phosphate 0.6g/L, ferrous sulfate heptahydrate 0.4g/L, slowly intermittent addition sodium hydroxide is adjusted to pH6, cultivates
Basigamy system is completed and culture medium is placed in Anaerobic culturel bottle mesohigh steam sterilizing, and sterilize pressure 105kPa, 118 DEG C of temperature, goes out
Bacterium 25min；
It d. is 2 according to mass ratio by heavy metal waste slag contaminated soil and culture medium:0.3mL/g acquisition is added in 1 mixing
The strain liquid arrived is cultivated 3 days, has black precipitate appearance, illustrate that growth is good, and strain is sulfate reduction strain；
E. under aseptic technique, the sulfate reducing bacteria strain liquid screened is added into sterilized culture medium
0.1mL/g, sealing and standing expand culture 5 days, obtain the sulfate reducing bacteria that can be used for heavy-metal contaminated soil reparation；
F. by bacillus subtilis microbial agent and water according to mass ratio 1:500 are made into bacillus subtilis bacterium solution, then withered grass
Bacillus bacterium solution and the sulfate reducing bacteria of expansion culture are according to 5:1 ratio carries out being mixed and made into mixed bacteria；
G. at normal temperature, culture medium solution pH is 6 after regulating step (4) sterilizing, then will be after mixed bacteria liquid and sterilizing
Culture medium solution is according to 1：10 accesses mix, to heavy metal waste slag stockpiling place soil according to 0.6L/m2Amount sprinkling access it is mixed
Then the culture medium solution of combined bacteria kind carries out heavy metal waste slag and stores up place soil remediation, simultaneous selection sub-fraction heavy metal
Waste residue stockpiling place sprinkling equivalent does not access the culture medium of mixed bacteria liquid as a control group；
H. concentration of heavy metal ion is measured：Every pH in 5 days measurement heavy metal waste slags stockpiling place soil solution and lead,
Zinc, cadmium metal ion concentration, record numerical value is as follows, and continues to add culture medium solution 0.6L/m after step (4) sterilize2, repair
After 20 days multiple, the soil repaired, which seeps concentration of heavy metal ion in filtrate, can reach GB25466-2010《Lead, Zn Cr coating pollution
Object discharge standard》Standard.
Implementation case 2
A. acidophilus strain and sulfate reduction strain are acquired from nonferrous metals in Guangxi Mineral Processing Enterprises Tailings Dam soil；
B. sweet sorghum stalk crushing is placed in fermentor, with lactic acid bacteria proportionally 300:1 mixing, adds water and stirs
It is even, make water tariff collection 30%, spontaneous fermentation 4 days are spare；
C. after fermentation, the sweet sorghum stalk of fermentation is taken out, epsom salt 0.6g/L, ammonium sulfate 0.9g/ is added
L, potassium dihydrogen phosphate 0.4g/L, ferrous sulfate heptahydrate 0.6g/L, slowly intermittent addition sodium hydroxide is adjusted to pH8, cultivates
Basigamy system is completed and culture medium is placed in Anaerobic culturel bottle mesohigh steam sterilizing, and sterilize pressure 101kPa, 123 DEG C of temperature, goes out
Bacterium 20min；
It d. is 3 according to mass ratio by heavy metal waste slag contaminated soil and culture medium:0.6mL/g acquisition is added in 1 mixing
The strain liquid arrived is cultivated 4 days, has black precipitate appearance, illustrate that growth is good, and strain is sulfate reduction strain；
E. under aseptic technique, the sulfate reducing bacteria strain liquid screened is added into sterilized culture medium
0.5mL/g, sealing and standing expand culture 3 days, obtain the sulfate reducing bacteria that can be used for heavy-metal contaminated soil reparation；
F. by bacillus subtilis microbial agent and water according to mass ratio 1:600 are made into bacillus subtilis bacterium solution, then withered grass
Bacillus bacterium solution and the sulfate reducing bacteria of expansion culture are according to 6:1 ratio carries out being mixed and made into mixed bacteria；
G. at normal temperature, culture medium solution pH is 6 after regulating step (4) sterilizing, then will be after mixed bacteria liquid and sterilizing
Culture medium solution is according to 1：11 accesses mix, to heavy metal waste slag stockpiling place soil according to 0.7L/m2Amount sprinkling access it is mixed
Then the culture medium solution of combined bacteria kind carries out heavy metal waste slag and stores up place soil remediation, simultaneous selection sub-fraction heavy metal
Waste residue stockpiling place sprinkling equivalent does not access the culture medium of mixed bacteria liquid as a control group；
H. concentration of heavy metal ion is measured：Every pH in 6 days measurement heavy metal waste slags stockpiling place soil solution and lead,
Zinc, cadmium metal ion concentration, record numerical value is as follows, and continues to add culture medium solution 0.7L/m after step (4) sterilize2, repair
After 24 days multiple, the soil repaired, which seeps concentration of heavy metal ion in filtrate, can reach GB25466-2010《Lead, Zn Cr coating pollution
Object discharge standard》Standard.
Embodiment 3
A. acidophilus strain and sulfate reduction strain are acquired from nonferrous metals in Guangxi Mineral Processing Enterprises Tailings Dam soil；
B. sweet sorghum stalk crushing is placed in fermentor, with lactic acid bacteria proportionally 250:1 mixing, adds water and stirs
It is even, make water tariff collection 40%, spontaneous fermentation 4 days are spare；
C. after fermentation, the sweet sorghum stalk of fermentation is taken out, epsom salt 0.5g/L, ammonium sulfate 1.0g/ is added
L, potassium dihydrogen phosphate 0.5g/L, ferrous sulfate heptahydrate 0.5g/L, slowly intermittent addition sodium hydroxide is adjusted to pH7, cultivates
Basigamy system is completed and culture medium is placed in Anaerobic culturel bottle mesohigh steam sterilizing, and sterilize pressure 103kPa, 121 DEG C of temperature, goes out
Bacterium 20min；
It d. is 2 according to mass ratio by heavy metal waste slag contaminated soil and culture medium:0.4mL/g acquisition is added in 1 mixing
The strain liquid arrived is cultivated 4 days, has black precipitate appearance, illustrate that growth is good, and strain is sulfate reduction strain；
E. under aseptic technique, the sulfate reducing bacteria strain liquid screened is added into sterilized culture medium
0.6mL/g, sealing and standing expand culture 3 days, obtain the sulfate reducing bacteria that can be used for heavy-metal contaminated soil reparation；
F. by bacillus subtilis microbial agent and water according to mass ratio 1:700 are made into bacillus subtilis bacterium solution, then withered grass
Bacillus bacterium solution and the sulfate reducing bacteria of expansion culture are according to 7:1 ratio carries out being mixed and made into mixed bacteria；
G. at normal temperature, culture medium solution pH is 7 after regulating step (4) sterilizing, then will be after mixed bacteria liquid and sterilizing
Culture medium solution is according to 1：12 accesses mix, to heavy metal waste slag stockpiling place soil according to 0.8L/m2Amount sprinkling access it is mixed
Then the culture medium solution of combined bacteria kind carries out heavy metal waste slag and stores up place soil remediation, simultaneous selection sub-fraction heavy metal
Waste residue stockpiling place sprinkling equivalent does not access the culture medium of mixed bacteria liquid as a control group；
H. concentration of heavy metal ion is measured：Every pH in 5 days measurement heavy metal waste slags stockpiling place soil solution and lead,
Zinc, cadmium metal ion concentration, record numerical value is as follows, and continues to add culture medium solution 0.8L/m after step (4) sterilize2, repair
After 20 days multiple, the soil repaired, which seeps concentration of heavy metal ion in filtrate, can reach GB25466-2010《Lead, Zn Cr coating pollution
Object discharge standard》Standard.
Embodiment 4
A. acidophilus strain and sulfate reduction strain are acquired from nonferrous metals in Guangxi Mineral Processing Enterprises Tailings Dam soil；
B. sweet sorghum stalk crushing is placed in fermentor, with lactic acid bacteria proportionally 250:1 mixing, adds water and stirs
It is even, make water tariff collection 50%, spontaneous fermentation 4 days are spare；
C. after fermentation, the sweet sorghum stalk of fermentation is taken out, epsom salt 0.5g/L, ammonium sulfate 1.1g/ is added
L, potassium dihydrogen phosphate 0.5g/L, ferrous sulfate heptahydrate 0.5g/L, slowly intermittent addition sodium hydroxide is adjusted to pH7, cultivates
Basigamy system is completed and culture medium is placed in Anaerobic culturel bottle mesohigh steam sterilizing, and sterilize pressure 105kPa, 120 DEG C of temperature, goes out
Bacterium 20min；
It d. is 3 according to mass ratio by heavy metal waste slag contaminated soil and culture medium:0.3mL/g acquisition is added in 1 mixing
The strain liquid arrived is cultivated 5 days, has black precipitate appearance, illustrate that growth is good, and strain is sulfate reduction strain；
E. under aseptic technique, the sulfate reducing bacteria strain liquid screened is added into sterilized culture medium
0.5mL/g, sealing and standing expand culture 3 days, obtain the sulfate reducing bacteria that can be used for heavy-metal contaminated soil reparation；
F. by bacillus subtilis microbial agent and water according to mass ratio 1:800 are made into bacillus subtilis bacterium solution, then withered grass
Sulfate reducing bacteria after bacillus bacterium solution and expansion culture is according to 10:1 ratio carries out being mixed and made into mixed bacteria；
G. at normal temperature, culture medium solution pH is 7 after regulating step (4) sterilizing, then will be after mixed bacteria liquid and sterilizing
Culture medium solution is according to 1：10 accesses mix, to heavy metal waste slag stockpiling place soil according to 0.8L/m2Amount sprinkling access it is mixed
Then the culture medium solution of combined bacteria kind carries out heavy metal waste slag and stores up place soil remediation, simultaneous selection sub-fraction heavy metal
Waste residue stockpiling place sprinkling equivalent does not access the culture medium of mixed bacteria liquid as a control group；
H. concentration of heavy metal ion is measured：Every pH in 7 days measurement heavy metal waste slags stockpiling place soil solution and lead,
Zinc, cadmium metal ion concentration, record numerical value is as follows, and continues to add culture medium solution 0.8L/m after step (4) sterilize2, repair
After 28 days multiple, the soil repaired, which seeps concentration of heavy metal ion in filtrate, can reach GB25466-2010《Lead, Zn Cr coating pollution
Object discharge standard》Standard.
Above embodiments are only exemplary embodiment of the present invention, are not used in the limitation present invention, protection scope of the present invention
It is defined by the claims.Those skilled in the art can make various repair to the present invention within the spirit and scope of the present invention
Change or equivalent replacement, this modification or equivalent replacement also should be regarded as being within the scope of the present invention.
Claims (8)
1. it is a kind of containing heavy metal waste slag stockpiling place repairing method of microorganism, which is characterized in that using containing heavy metal waste slag as
Medium filters out the sulfate reducing bacteria survived in the hazardous waste soil for adapting to heavy metal, is added and passes through anaerobic lactic acid fermentation
Sweet sorghum stalk and the culture medium that mixes of nutrients, adjust medium pH 6-8, then remix bacillus subtilis
It carries out heavy metal waste slag and stores up place soil remediation, soil reaches normal crop planting nutritional need, includes the following steps：
(1) strain acquires：It is acquired from nonferrous metals in Guangxi Mineral Processing Enterprises Tailings Dam soil, acquires sulfate reducing bacteria；
(2) it ferments：By sweet sorghum stalk crushing be placed in it is closed in fermentor, carried out under room temperature lactic fermentation 8-12 days it is spare；
(3) prepared by culture medium：Other nutritional ingredients are added into sweet sorghum stalk fermentation liquid, culture medium is made, adjusts medium pH
6-8；
(4) medium sterilization：Culture medium is placed in Anaerobic culturel bottle mesohigh steam sterilizing, sterilize 101~105kPa of pressure, temperature
118-123 DEG C of degree, sterilization time 15-25min；
(5) screening of sulfate reducing bacteria：It is proportionally added into the soil containing heavy metal waste slag in the medium, then in the medium
Collected strain in 0.3~0.6mL/g step (1) is added, visible black color precipitates after culture 3-4 days, illustrates that growth is good
It is good, then filter out good sulfate reducing bacteria；
(6) sulfate reducing bacteria expands culture：Under aseptic technique, step (5) screening is added into sterilized culture medium
Sulfate reducing bacteria 0.1~0.5mL/g of bacterium solution out, sealing and standing expand culture 3-5 days；
(7) by bacillus subtilis microbial agent and water according to mass ratio 1:500~800 are made into bacillus subtilis bacterium solution, withered grass bud
Spore bacillus bacterium solution and the sulfate reducing bacteria expanded after culture carry out being mixed and made into mixed bacteria liquid according to a certain percentage；
(8) remediating heavy metal soil：At normal temperature, culture medium solution pH is 6-7 after regulating step (4) sterilizing, then will be mixed
Culture medium solution is according to 1 after combined bacteria liquid and sterilizing：10~12 access mix, then to heavy metal waste slag stockpiling place soil according to
0.6~0.8L/m2Amount sprinkling access mixed bacteria culture medium solution, carry out heavy metal waste slag store up place soil remediation；
(9) concentration of heavy metal ion is measured：Every pH in 5-7 days measurement heavy metal waste slags stockpiling place soil solution and lead,
Zinc, cadmium metal ion concentration, and continue to add 0.6~0.8L/m of culture medium solution after step (4) sterilize2, repair 20-28 days
Afterwards, soil, which seeps concentration of heavy metal ion in filtrate, can reach GB25466-2010《Lead, Zn Cr coating pollutant emission standard》's
Standard.
2. the repairing method of microorganism in the place according to claim 1 containing heavy metal waste slag stockpiling, it is characterised in that：It is described
Step (2) sweet sorghum stalk powder and lactic acid bacteria proportionally 200~300:1 mixing and water adding stirs evenly, make water tariff collection 30~
50%, sealed fermenting.
3. the repairing method of microorganism in the place according to claim 1 containing heavy metal waste slag stockpiling, it is characterised in that：It is described
Step (3) be added nutritional ingredient be：0.4~0.6g/L of epsom salt, 0.9~1.2g/L of ammonium sulfate, potassium dihydrogen phosphate 0.4
~0.6g/L, 0.4~0.6g/L of ferrous sulfate heptahydrate.
4. the repairing method of microorganism in the place according to claim 1 containing heavy metal waste slag stockpiling, it is characterised in that：It is described
Step (3) adjusts medium pH using sodium hydroxide is slowly intermittent.
5. the repairing method of microorganism in the place according to claim 1 containing heavy metal waste slag stockpiling, it is characterised in that：It is described
The mass ratio of heavy metal waste slag is added in step (5) soil and culture medium is 2~3:1.
6. the repairing method of microorganism in the place according to claim 1 containing heavy metal waste slag stockpiling, it is characterised in that：It is described
Step (7) mixed bacteria is bacillus subtilis bacterium solution and sulfate reducing bacteria according to mass ratio 5~10:1 is mixed.
7. the repairing method of microorganism in the place according to claim 1 containing heavy metal waste slag stockpiling, it is characterised in that：It is described
Step (8) initial pH value is adjusted using sodium hydroxide solution.
8. the repairing method of microorganism in the place according to claim 1 containing heavy metal waste slag stockpiling, it is characterised in that：It is described
Step (9) is described to use heavy metal element type and content in AFS DETERMINATION leachate.
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| CN111675434A (en) * | 2020-06-17 | 2020-09-18 | 盐城工学院 | Treatment method of arsenic-containing wastewater in copper smelting |
| CN112940730A (en) * | 2021-01-15 | 2021-06-11 | 华北地质勘查局五一四地质大队 | Agent for repairing contaminated soil of mine heavy metal tailing pond and using method |
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| CN113583683A (en) * | 2021-08-27 | 2021-11-02 | 中钢集团马鞍山矿山研究总院股份有限公司 | Soil conditioner for acidified tailing pond |
| CN114570764A (en) * | 2022-04-20 | 2022-06-03 | 中南大学 | Microbial remediation method for polluted soil in antimony ore region |
| CN114749479A (en) * | 2022-05-30 | 2022-07-15 | 中南大学 | Method for repairing arsenic-containing gold tailings by using plant-microorganism combination |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101642772A (en) * | 2009-08-26 | 2010-02-10 | 王颖 | Microbe soil restoration agent and preparation method thereof |
| CN102925348A (en) * | 2012-11-11 | 2013-02-13 | 中国船舶重工集团公司第七二五研究所 | Device for detecting concentration of sulfate-reducing bacteria (SRB) |
| CN104450552A (en) * | 2014-08-17 | 2015-03-25 | 西北大学 | Sulfate reducing bacteria-phosphate solubilizing bacteria and application thereof in combined remediation of cadmium contaminated soil |
| CN105331564A (en) * | 2015-12-14 | 2016-02-17 | 甘肃省科学院生物研究所 | Immobilized complex microbial agent and method of directly utilizing immobilized complex microbial agent for fermenting sweet sorghum straw to make feedstuff |
| CN106417369A (en) * | 2016-09-21 | 2017-02-22 | 苏州直角新材料有限公司 | Multipurpose sterilizing agent |
| CN107488620A (en) * | 2017-10-08 | 2017-12-19 | 中国科学院成都生物研究所 | A kind of cadmium pollution paddy soil microorganism passivator |
-
2018
- 2018-06-07 CN CN201810582925.XA patent/CN108856283B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101642772A (en) * | 2009-08-26 | 2010-02-10 | 王颖 | Microbe soil restoration agent and preparation method thereof |
| CN102925348A (en) * | 2012-11-11 | 2013-02-13 | 中国船舶重工集团公司第七二五研究所 | Device for detecting concentration of sulfate-reducing bacteria (SRB) |
| CN104450552A (en) * | 2014-08-17 | 2015-03-25 | 西北大学 | Sulfate reducing bacteria-phosphate solubilizing bacteria and application thereof in combined remediation of cadmium contaminated soil |
| CN105331564A (en) * | 2015-12-14 | 2016-02-17 | 甘肃省科学院生物研究所 | Immobilized complex microbial agent and method of directly utilizing immobilized complex microbial agent for fermenting sweet sorghum straw to make feedstuff |
| CN106417369A (en) * | 2016-09-21 | 2017-02-22 | 苏州直角新材料有限公司 | Multipurpose sterilizing agent |
| CN107488620A (en) * | 2017-10-08 | 2017-12-19 | 中国科学院成都生物研究所 | A kind of cadmium pollution paddy soil microorganism passivator |
Non-Patent Citations (1)
| Title |
|---|
| 李明: "《兰花菌根研究及应用》", 31 May 2006 * |
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| CN110026431A (en) * | 2019-05-20 | 2019-07-19 | 成都科泰技术有限公司 | A method of utilizing microorganism nano material in-situ cadmium pollution soil repair |
| CN110026431B (en) * | 2019-05-20 | 2020-12-22 | 成都科泰技术有限公司 | Method for in-situ remediation of cadmium-polluted soil by using microbial nano-material |
| CN111675434A (en) * | 2020-06-17 | 2020-09-18 | 盐城工学院 | Treatment method of arsenic-containing wastewater in copper smelting |
| CN112940730A (en) * | 2021-01-15 | 2021-06-11 | 华北地质勘查局五一四地质大队 | Agent for repairing contaminated soil of mine heavy metal tailing pond and using method |
| CN113231457A (en) * | 2021-04-30 | 2021-08-10 | 合肥工业大学 | Method for restoring heavy metal polluted soil by magnesium oxide and microorganisms |
| CN113231457B (en) * | 2021-04-30 | 2023-03-17 | 合肥工业大学 | Method for restoring heavy metal polluted soil by magnesium oxide and microorganisms |
| CN113351641A (en) * | 2021-06-23 | 2021-09-07 | 西南科技大学 | Method for remedying heavy metal pollution of smelting slag through non-covering organisms |
| CN113351642A (en) * | 2021-07-07 | 2021-09-07 | 中国地质大学（北京） | Repair method for repairing antimony and zinc combined pollution and application |
| CN113583683A (en) * | 2021-08-27 | 2021-11-02 | 中钢集团马鞍山矿山研究总院股份有限公司 | Soil conditioner for acidified tailing pond |
| CN114570764A (en) * | 2022-04-20 | 2022-06-03 | 中南大学 | Microbial remediation method for polluted soil in antimony ore region |
| CN114749479A (en) * | 2022-05-30 | 2022-07-15 | 中南大学 | Method for repairing arsenic-containing gold tailings by using plant-microorganism combination |
| CN117655092A (en) * | 2024-01-05 | 2024-03-08 | 成都市市政开发总公司 | Soil heavy metal pollution restoration method based on biological action |
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