CN108856283B - Microbial remediation method for heavy metal-containing waste residue stockyard - Google Patents
Microbial remediation method for heavy metal-containing waste residue stockyard Download PDFInfo
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Abstract
The invention discloses a method for repairing microorganisms in a heavy metal-containing waste residue storage site, which is characterized in that sulfate reducing bacteria are domesticated by using hazardous waste containing heavy metals such as lead, zinc, cadmium and the like as a culture medium, so that the survival capability and the environmental adaptability of the sulfate reducing bacteria in the hazardous waste containing the heavy metals such as lead, zinc, cadmium, arsenic and the like are improved; various nutrient substances are added to ensure that the soil restoration by mixing the sulfate reducing bacteria and the bacillus subtilis is strengthened, the pollutants are rapidly removed, and the planting capability of soil crops is recovered.
Description
Technical Field
The invention relates to a heavy metal waste residue storage site and a method for repairing the pollution of surrounding soil, belonging to the field of environmental protection.
Background
The pollution remediation of the stockpiling site and the periphery of the waste residues containing heavy metals such as cadmium, lead, zinc and the like needs to be paid attention. Because some dangerous wastes are produced and disposed, and pollution precautionary measures of enterprises are not in place, the typical dangerous wastes are leaked and scattered in the long-term storage and transfer process, the site and surrounding soil are polluted, even underground water is polluted, the cost for repairing the polluted site by adopting a traditional mode is huge, and common enterprises cannot bear the pollution, so that the polluted site exists for a long time, pollutants continuously diffuse along with rainfall and rain, the environment is polluted by a larger area, and the health of people is threatened. Therefore, there is a strong urgency to develop economically feasible, technically reliable site remediation technologies to prevent the continued spread of hazardous waste contamination.
The treatment modes of heavy metal pollution caused by hazardous waste mainly comprise physical treatment, chemical treatment and biological treatment. Physical and chemical remediation methods are not ideal treatment methods because they are not only costly, but also tend to change the properties of the soil, resulting in reduced yield of plants, and are not conducive to sustainable development. The bioremediation of heavy metal pollution in hazardous waste storage sites mainly comprises microbial remediation and phytoremediation. Phytoremediation is also a promising heavy metal pollution remediation technology at present, but the difficulty of phytoremediation lies in: due to chemical conditions and heavy metal toxins which are not suitable for the survival of plants, the survival rate of the plants is low, the inventory time is short, and a sustainable plant ecosystem is difficult to establish. ② the slow heavy metal removal rate and incomplete heavy metal metabolism limit the application of phytoremediation. It is also emphasized that the above methods only aim at the site which is polluted by heavy metal (no new heavy metal pollution source), and are difficult to be applied to the treatment of the polluted site (dangerous waste stockpiling site) in which the heavy metal source is continuously dissolved out. The microbial remediation is not only suitable for remediation of soil polluted by heavy metal, but also suitable for remediation of sites where heavy metal ions are continuously dissolved out, and has the characteristics of low cost, good remediation effect, small damage to the environment and the soil structure, contribution to ecological restoration and the like, so that the microbial remediation has larger development space and application prospect compared with the microbial remediation.
At present, the microorganism remediation of heavy metal pollution by using microorganisms has been backup attempts at home and abroad. The processing principle is as follows:
H2S+M2+→MS↓+2H+
wherein M is metal cation such as Fe, Ni, Cu, Cd, Cr, Zn, etc.
The microorganism remediation method for heavy metal pollution is mainly characterized in that some microorganisms have sulfate reducing capacity and can recover
Reduction to S2-Reaction product S2-React with heavy metal ions dissolved in the ore to form precipitates, so that the soluble heavy metal is immobilized and stabilized. The method for treating heavy metals by sulfate reducing bacteria has the advantages of low treatment cost, multiple types of treated heavy metals, strong applicability and no secondary pollution.
Most bacilli belong to gram-positive bacteria, and spores are produced in the absence of nutrients and in poor environments. Many researches find that the bacillus has obvious adsorption effect on heavy metal ions, has strong resistance to heavy metals such as Cd, Pb, Cr and the like, and the cell wall of the bacillus subtilis contains a large amount of peptidoglycan and teichoic acid, so that a large amount of active groups such as carboxyl, acylamino and the like can be provided, and the groups can lose protons to enable the surface of the thallus to have strong negative charges so as to enable the thallus to adsorb the heavy metal ions by electrostatic attraction.
Disclosure of Invention
According to the method, hazardous wastes of heavy metals such as lead, zinc, cadmium, arsenic and the like are used as culture media, sulfate reducing bacteria are domesticated, the viability and the environmental adaptability of the sulfate reducing bacteria in the hazardous wastes of the heavy metals such as lead, zinc, cadmium, arsenic and the like are improved, the large-scale culture of the sulfate reducing bacteria is optimized, and the large-scale remediation of heavy metal pollution in-situ and surrounding soil of the hazardous waste storage site containing the heavy metals such as lead, zinc, cadmium, arsenic and the like is carried out.
The purpose of the invention is realized by the following technical scheme:
a microorganism restoration method for a heavy metal-containing waste residue storage site comprises the following steps of screening out sulfate reducing bacteria suitable for the survival of heavy metal dangerous waste soil by using heavy metal-containing waste residues as a medium, adding a culture medium formed by mixing sweet sorghum stalks subjected to anaerobic lactic acid fermentation and nutrients, adjusting the pH of the culture medium to 6-8, mixing bacillus subtilis to restore the heavy metal waste residue storage site soil, and enabling the soil to meet the nutritional requirements of normal crop planting, wherein the method comprises the following steps:
(1) collecting strains: collecting soil from a tailing pond of Guangxi nonferrous metal beneficiation enterprises;
(2) fermentation: pulverizing sweet sorghum stalks, sealing in a fermentation tank, and performing lactic acid fermentation at normal temperature for 8-12 days for later use;
(3) preparing a culture medium: adding other nutrient components into the sweet sorghum stalk fermentation liquor to prepare a culture medium, and adjusting the pH value of the culture medium to 6-8;
(4) and (3) sterilizing a culture medium: placing the culture medium in an anaerobic culture bottle, and sterilizing by high-pressure steam at the sterilization pressure of 101-105 kPa and the temperature of 118-;
(5) screening of sulfate reducing bacteria: adding soil containing heavy metal waste residues into a culture medium according to a proportion, adding 0.3-0.6 mL/g of the strain collected in the step (1) into the culture medium, and culturing for 3-4 days until black precipitates are visible, so that good sulfate reducing bacteria are screened out if the strain grows well;
(6) and (3) carrying out expanded culture on sulfate reducing bacteria: under the aseptic operation condition, adding 0.1-0.5 mL/g of sulfate reducing bacteria liquid screened in the step (5) into a sterilized culture medium, sealing, standing and expanding for culture for 3-5 days;
(7) preparing a bacillus subtilis microbial inoculum and water into bacillus subtilis liquid according to the mass ratio of 1: 500-800, and mixing the bacillus subtilis liquid and the sulfate reducing bacteria subjected to amplification culture according to a certain proportion to prepare a mixed strain;
(8) repairing heavy metal soil: at normal temperature, adjusting the pH value of the culture medium solution after sterilization in the step (4) to 6-7, and then mixing the mixed bacterial liquid and the sterilized culture medium solution according to the ratio of 1: 10-12, uniformly mixing, and adding the mixture into the soil of the heavy metal waste residue storage site according to the proportion of 0.6-0.8L/m2Spraying a culture medium solution inoculated with the mixed strain, and then performing soil remediation on the heavy metal waste residue stockpiling site;
(9) and (3) determining the concentration of heavy metal ions: and (3) determining the concentration of heavy metal ions: measuring the pH value and the concentrations of lead, zinc and cadmium metal ions in the soil percolate of the heavy metal waste residue storage site every 5 to 7 days, and continuously adding 0.6 to 0.8L/m of the culture medium solution after the sterilization in the step (4)2And after 20-28 days of remediation, the concentration of heavy metal ions in the soil leachate can reach the standard of GB25466-2010 lead and zinc industrial pollutant emission standard.
Preferably, in the step (2), the sweet sorghum stalk powder and the lactic acid bacteria are mixed according to a ratio of 200-300: 1, water is added, the mixture is uniformly stirred, the water content is kept at 30-50%, and the mixture is subjected to closed fermentation.
Preferably, the nutrient components added in the step (3) are as follows: 0.4-0.6 g/L magnesium sulfate heptahydrate, 0.9-1.2 g/L ammonium sulfate, 0.4-0.6 g/L monopotassium phosphate and 0.4-0.6 g/L ferrous sulfate heptahydrate, and the multi-element nutrition is beneficial to growth of the growth solution of the sulfate reducing bacteria and is suitable for growth of bacillus subtilis.
Preferably, in the step (3), sodium hydroxide is used to slowly and intermittently adjust the pH of the medium, so that sodium hydroxide and the fermented lactic acid can sufficiently react to obtain sodium lactate.
Preferably, the mass ratio of the soil added with the heavy metal waste residue in the step (5) to the culture medium is 2-3: 1, and under the condition of the mass ratio, the nutrient components in the culture medium are utilized to the maximum extent while the heavy metal ions in the soil are effectively removed.
Preferably, in the step (7), the mixed strain is prepared by mixing the bacillus subtilis solution and the sulfate reducing bacteria according to the mass ratio of 5-10: 1.
Preferably, the initial pH value in step (8) is adjusted by using sodium hydroxide solution.
Preferably, in the step (9), the species and the content of the heavy metal elements in the leachate are measured by using atomic fluorescence spectrometry, and the atomic fluorescence spectrometry has high sensitivity, wide linear range of a calibration curve and capability of performing multi-element simultaneous measurement.
The bacillus subtilis microbial inoculum can be purchased in the market.
The invention has the beneficial effects that:
1. compared with the plant remediation of the soil with heavy metal pollution and the field soil with the heavy metal continuously dissolved out, the method has the advantages of quick effect, better effect and low cost, the traditional plant remediation of the soil with heavy metal pollution can not be realized, and the plant is difficult to survive.
2. According to the invention, sulfate reducing bacteria and bacillus subtilis are mixed to repair microorganisms in a heavy metal waste residue storage site, the sulfate reducing bacteria have a synergistic effect on the bacillus subtilis in the process of repairing heavy metals in soil, the effect of the bacillus subtilis on the adsorption capacity of the heavy metals in the soil is better than that of the bacillus subtilis when used alone, and the corresponding sulfate reducing bacteria treat the heavy metals adsorbed by the bacillus subtilis, so that the efficiency of repairing the heavy metal polluted soil is higher and the effect is better than that of the sulfate reducing bacteria used alone.
3. The sweet sorghum stalks are used as a main carbon source of sulfate reducing bacteria, and the pH value is adjusted by using sodium hydroxide, so that sodium lactate is obtained by the reaction of the sodium hydroxide and lactic acid fermented by the sweet sorghum stalks, the reduction rate of the sulfate reducing bacteria is improved, and the sodium lactate can be used as a nutrient source of bacillus subtilis, so that the raw materials are cheap and easy to obtain, and the cost is saved.
4. The invention directly repairs the polluted soil, simultaneously solves the problem that the soil repaired by the source of the underground water polluted by the heavy metal can be used for planting vegetation or crops, realizes the comprehensive treatment of the land resource environment in the process of diversifying the nutrition of the soil and improving the soil by the bacillus subtilis, and builds an environment-friendly society.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
a. Collecting acidophilic bacteria and sulfate reducing bacteria from the soil of tailings pond of Guangxi nonferrous metal mineral processing enterprise;
b. pulverizing sweet sorghum stalks, placing in a fermentation tank, mixing with lactobacillus at a ratio of 200:1, adding water, stirring to keep water content at 50%, and fermenting naturally in a closed state for 3 days;
c. after fermentation, taking out sweet sorghum stalk fermentation liquor, adding 0.4g/L magnesium sulfate heptahydrate, 1.2g/L ammonium sulfate, 0.6g/L potassium dihydrogen phosphate and 0.4g/L ferrous sulfate heptahydrate, slowly and intermittently adding sodium hydroxide to adjust the pH to 6, finishing culture medium preparation, placing the culture medium in an anaerobic culture bottle, and sterilizing for 25min by high-pressure steam at the sterilization pressure of 105kPa and the temperature of 118 ℃;
d. mixing the soil polluted by the heavy metal waste residue with a culture medium according to the mass ratio of 2:1, adding 0.3mL/g of collected strain liquid, culturing for 3 days, wherein black precipitates appear, which indicates that the strain grows well, and the strain is a sulfate reducing strain;
e. under the aseptic operation condition, adding 0.1mL/g of screened sulfate reducing bacteria liquid into a sterilized culture medium, sealing, standing and expanding for culture for 5 days to obtain sulfate reducing bacteria for repairing heavy metal contaminated soil;
f. preparing a bacillus subtilis microbial inoculum and water into bacillus subtilis liquid according to the mass ratio of 1:500, and then mixing the bacillus subtilis liquid and the sulfate reducing bacteria subjected to amplification culture according to the proportion of 5:1 to prepare a mixed strain;
g. and (3) adjusting the pH value of the culture medium solution after sterilization in the step (4) to 6 at normal temperature, and then mixing the mixed bacterial liquid and the sterilized culture medium solution according to the ratio of 1: 10, uniformly mixing, and adding the mixture into the soil of the heavy metal waste residue storage field according to the proportion of 0.6L/m2Spraying a culture medium solution inoculated with the mixed strain, then performing soil remediation on the heavy metal waste residue storage site, and simultaneously selecting a small part of the heavy metal waste residue storage site to spray an equivalent culture medium which is not inoculated with the mixed strain solution as a control group;
h. and (3) determining the concentration of heavy metal ions: measuring the pH value and the concentrations of lead, zinc and cadmium metal ions in the soil percolate of the heavy metal waste residue storage site every 5 days, recording the numerical values as follows, and continuously adding 0.6L/m of culture medium solution after the sterilization in the step (4)2And after 20 days of remediation, the concentration of heavy metal ions in the remediated soil leachate can reach the standard of GB25466-2010 lead and zinc industry pollutant emission standard.
Best mode for carrying out the invention
a. Collecting acidophilic bacteria and sulfate reducing bacteria from the soil of tailings pond of Guangxi nonferrous metal mineral processing enterprise;
b. pulverizing sweet sorghum stalks, placing in a fermentation tank, mixing with lactobacillus at a ratio of 300:1, adding water, stirring to keep water content at 30%, and naturally fermenting for 4 days;
c. after fermentation, taking out the fermented sweet sorghum stalks, adding 0.6g/L magnesium sulfate heptahydrate, 0.9g/L ammonium sulfate, 0.4g/L potassium dihydrogen phosphate and 0.6g/L ferrous sulfate heptahydrate, slowly and intermittently adding sodium hydroxide to adjust the pH to 8, finishing the preparation of the culture medium, placing the culture medium in an anaerobic culture bottle, and sterilizing the culture medium by high-pressure steam at the sterilization pressure of 101kPa and the temperature of 123 ℃ for 20 min;
d. mixing the soil polluted by the heavy metal waste residue with a culture medium according to the mass ratio of 3:1, adding 0.6mL/g of collected strain liquid, culturing for 4 days, wherein black precipitates appear, which indicates that the strain grows well, and the strain is a sulfate reducing strain;
e. under the aseptic operation condition, adding 0.5mL/g of screened sulfate reducing bacteria liquid into a sterilized culture medium, sealing, standing and expanding for culture for 3 days to obtain sulfate reducing bacteria for repairing heavy metal contaminated soil;
f. preparing a bacillus subtilis microbial inoculum and water into bacillus subtilis liquid according to the mass ratio of 1:600, and then mixing the bacillus subtilis liquid and the sulfate reducing bacteria subjected to amplification culture according to the ratio of 6:1 to prepare a mixed strain;
g. and (3) adjusting the pH value of the culture medium solution after sterilization in the step (4) to 6 at normal temperature, and then mixing the mixed bacterial liquid and the sterilized culture medium solution according to the ratio of 1: 11, uniformly mixing, and adding the mixture into the soil of the heavy metal waste residue storage field according to the proportion of 0.7L/m2Spraying a culture medium solution inoculated with the mixed strain, then performing soil remediation on the heavy metal waste residue storage site, and simultaneously selecting a small part of the heavy metal waste residue storage site to spray an equivalent culture medium which is not inoculated with the mixed strain solution as a control group;
h. and (3) determining the concentration of heavy metal ions: measuring the pH value and the concentrations of lead, zinc and cadmium metal ions in the soil percolate of the heavy metal waste residue storage site every 6 days, recording the numerical values as follows, and continuously adding 0.7L/m of culture medium solution after the sterilization in the step (4)2And after 24 days of remediation, the concentration of heavy metal ions in the remediated soil leachate can reach the standard of GB25466-2010 lead and zinc industry pollutant emission standard.
Example 3
a. Collecting acidophilic bacteria and sulfate reducing bacteria from the soil of tailings pond of Guangxi nonferrous metal mineral processing enterprise;
b. pulverizing sweet sorghum stalks, placing in a fermentation tank, mixing with lactobacillus at a ratio of 250:1, adding water, stirring to keep water content at 40%, and naturally fermenting for 4 days;
c. after fermentation, taking out the fermented sweet sorghum stalks, adding 0.5g/L magnesium sulfate heptahydrate, 1.0g/L ammonium sulfate, 0.5g/L potassium dihydrogen phosphate and 0.5g/L ferrous sulfate heptahydrate, slowly and intermittently adding sodium hydroxide to adjust the pH to 7, finishing the preparation of the culture medium, placing the culture medium in an anaerobic culture bottle, and sterilizing the culture medium by high-pressure steam at the sterilization pressure of 103kPa and the temperature of 121 ℃ for 20 min;
d. mixing the soil polluted by the heavy metal waste residue with a culture medium according to the mass ratio of 2:1, adding 0.4mL/g of collected strain liquid, culturing for 4 days, wherein black precipitates appear, which indicates that the strain grows well, and the strain is a sulfate reducing strain;
e. under the aseptic operation condition, adding 0.6mL/g of screened sulfate reducing bacteria liquid into a sterilized culture medium, sealing, standing and expanding for culture for 3 days to obtain sulfate reducing bacteria for repairing heavy metal contaminated soil;
f. preparing a bacillus subtilis microbial inoculum and water into bacillus subtilis liquid according to the mass ratio of 1:700, and then mixing the bacillus subtilis liquid and the sulfate reducing bacteria subjected to amplification culture according to the proportion of 7:1 to prepare a mixed strain;
g. and (3) adjusting the pH value of the culture medium solution after sterilization in the step (4) to 7 at normal temperature, and mixing the mixed bacterial liquid and the sterilized culture medium solution according to the ratio of 1: 12, uniformly mixing, and adding the mixture into the soil of the heavy metal waste residue storage field according to the proportion of 0.8L/m2Spraying a culture medium solution inoculated with the mixed strain, then performing soil remediation on the heavy metal waste residue storage site, and simultaneously selecting a small part of the heavy metal waste residue storage site to spray an equivalent culture medium which is not inoculated with the mixed strain solution as a control group;
h. and (3) determining the concentration of heavy metal ions:measuring the pH value and the concentrations of lead, zinc and cadmium metal ions in the soil percolate of the heavy metal waste residue storage site every 5 days, recording the numerical values as follows, and continuously adding 0.8L/m of culture medium solution after the sterilization in the step (4)2And after 20 days of remediation, the concentration of heavy metal ions in the remediated soil leachate can reach the standard of GB25466-2010 lead and zinc industry pollutant emission standard.
Example 4
a. Collecting acidophilic bacteria and sulfate reducing bacteria from the soil of tailings pond of Guangxi nonferrous metal mineral processing enterprise;
b. pulverizing sweet sorghum stalks, placing in a fermentation tank, mixing with lactobacillus at a ratio of 250:1, adding water, stirring to keep water content at 50%, and naturally fermenting for 4 days;
c. after fermentation, taking out the fermented sweet sorghum stalks, adding 0.5g/L magnesium sulfate heptahydrate, 1.1g/L ammonium sulfate, 0.5g/L potassium dihydrogen phosphate and 0.5g/L ferrous sulfate heptahydrate, slowly and intermittently adding sodium hydroxide to adjust the pH to 7, finishing the preparation of the culture medium, placing the culture medium in an anaerobic culture bottle, and sterilizing the culture medium by high-pressure steam at the sterilization pressure of 105kPa and the temperature of 120 ℃ for 20 min;
d. mixing the soil polluted by the heavy metal waste residue with a culture medium according to the mass ratio of 3:1, adding 0.3mL/g of collected strain liquid, culturing for 5 days, wherein black precipitates appear, which indicates that the strain grows well, and the strain is a sulfate reducing strain;
e. under the aseptic operation condition, adding 0.5mL/g of screened sulfate reducing bacteria liquid into a sterilized culture medium, sealing, standing and expanding for culture for 3 days to obtain sulfate reducing bacteria for repairing heavy metal contaminated soil;
f. preparing a bacillus subtilis microbial inoculum and water into bacillus subtilis liquid according to the mass ratio of 1:800, and then mixing the bacillus subtilis liquid and the sulfate reducing bacteria subjected to amplification culture according to the proportion of 10:1 to prepare a mixed strain;
g. in thatAnd (3) at normal temperature, adjusting the pH value of the culture medium solution after sterilization in the step (4) to 7, and mixing the mixed bacterial liquid and the sterilized culture medium solution according to the ratio of 1: 10, uniformly mixing, and adding the mixture into the soil of the heavy metal waste residue storage field according to the proportion of 0.8L/m2Spraying a culture medium solution inoculated with the mixed strain, then performing soil remediation on the heavy metal waste residue storage site, and simultaneously selecting a small part of the heavy metal waste residue storage site to spray an equivalent culture medium which is not inoculated with the mixed strain solution as a control group;
h. and (3) determining the concentration of heavy metal ions: measuring the pH value and the concentrations of lead, zinc and cadmium metal ions in the soil percolate of the heavy metal waste residue storage site every 7 days, recording the numerical values as follows, and continuously adding 0.8L/m of culture medium solution after the sterilization in the step (4)2And after 28 days of remediation, the concentration of heavy metal ions in the remediated soil leachate can reach the standard of GB25466-2010 lead and zinc industry pollutant emission standard.
The above embodiments are only exemplary embodiments of the present invention, and are not intended to limit the present invention, and the scope of the present invention is defined by the claims. Various modifications and equivalents may be made thereto by those skilled in the art within the spirit and scope of the present invention, and such modifications and equivalents should be considered as falling within the scope of the present invention.
Claims (7)
1. A microbial remediation method for a heavy metal-containing waste residue storage site is characterized in that heavy metal-containing waste residues are used as a medium, sulfate reducing bacteria suitable for the heavy metal and living in dangerous waste soil are screened out, a culture medium formed by mixing sweet sorghum stems subjected to anaerobic lactic acid fermentation and nutrients is added, the pH of the culture medium is adjusted to 6-8, then bacillus subtilis is mixed to restore the heavy metal waste residue storage site soil, and the soil meets the nutritional requirements of normal crop planting, and the method comprises the following steps:
(1) collecting strains: collecting soil from a tailing pond of a Guangxi nonferrous metal beneficiation enterprise, and collecting sulfate reducing bacteria;
(2) fermentation: pulverizing sweet sorghum stalks, sealing in a fermentation tank, and performing lactic acid fermentation at normal temperature for 8-12 days for later use;
(3) preparing a culture medium: adding other nutrient components into the sweet sorghum stalk fermentation liquor to prepare a culture medium, and adjusting the pH value of the culture medium to 6-8;
(4) and (3) sterilizing a culture medium: placing the culture medium in an anaerobic culture bottle, and sterilizing by high-pressure steam at the sterilization pressure of 101-105 kPa and the temperature of 118-;
(5) screening of sulfate reducing bacteria: adding soil containing heavy metal waste residues into a culture medium according to a proportion, adding 0.3-0.6 mL/g of the strain collected in the step (1) into the culture medium, and culturing for 3-4 days until black precipitates are visible, so that good sulfate reducing bacteria are screened out if the strain grows well;
(6) and (3) carrying out expanded culture on sulfate reducing bacteria: under the aseptic operation condition, adding 0.1-0.5 mL/g of sulfate reducing bacteria liquid screened in the step (5) into a sterilized culture medium, sealing, standing and expanding for culture for 3-5 days;
(7) preparing a bacillus subtilis microbial inoculum and water into bacillus subtilis liquid according to the mass ratio of 1: 500-800, and mixing the bacillus subtilis liquid and the sulfate reducing bacteria subjected to amplification culture according to a certain proportion to prepare mixed bacterial liquid; the mixed strain is prepared by mixing bacillus subtilis liquid and sulfate reducing bacteria according to the mass ratio of 5-10: 1;
(8) repairing heavy metal soil: at normal temperature, adjusting the pH value of the culture medium solution after sterilization in the step (4) to 6-7, and then mixing the mixed bacterial liquid and the sterilized culture medium solution according to the ratio of 1: 10-12 parts of the mixture are mixed uniformly, and then the mixture is added into the soil of the heavy metal waste residue storage site according to the proportion of 0.6-0.8L/m2Spraying a culture medium solution inoculated with the mixed strain, and performing soil remediation on the heavy metal waste residue stockyard;
(9) and (3) determining the concentration of heavy metal ions: measuring the pH value and the concentrations of lead, zinc and cadmium metal ions in the soil percolate of the heavy metal waste residue storage site every 5 to 7 days, and continuously adding 0.6 to 0.8L/m of the culture medium solution after the sterilization in the step (4)2After 20-28 days of restoration, soil infiltrationThe concentration of heavy metal ions in the filtrate can reach the standard of GB25466-2010 discharge Standard of pollutants for lead and zinc industry.
2. The microbial remediation method of a heavy metal-containing waste residue storage yard of claim 1, wherein: and (3) mixing the sweet sorghum stalk powder and the lactic acid bacteria according to the ratio of 200-300: 1, adding water, uniformly stirring to keep the water content at 30-50%, and performing closed fermentation.
3. The microbial remediation method of a heavy metal-containing waste residue storage yard of claim 1, wherein: the nutrient components added in the step (3) are as follows: 0.4-0.6 g/L magnesium sulfate heptahydrate, 0.9-1.2 g/L ammonium sulfate, 0.4-0.6 g/L potassium dihydrogen phosphate and 0.4-0.6 g/L ferrous sulfate heptahydrate.
4. The microbial remediation method of a heavy metal-containing waste residue storage yard of claim 1, wherein: and (3) slowly and intermittently adjusting the pH of the culture medium by using sodium hydroxide.
5. The microbial remediation method of a heavy metal-containing waste residue storage yard of claim 1, wherein: the mass ratio of the soil added with the heavy metal waste residues in the step (5) to the culture medium is 2-3: 1.
6. The microbial remediation method of a heavy metal-containing waste residue storage yard of claim 1, wherein: the initial pH value of the step (8) is adjusted by using a sodium hydroxide solution.
7. The microbial remediation method of a heavy metal-containing waste residue storage yard of claim 1, wherein: and (9) determining the types and the contents of the heavy metal elements in the leachate by using an atomic fluorescence spectrometry.
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| CN114570764A (en) * | 2022-04-20 | 2022-06-03 | 中南大学 | Microbial remediation method for polluted soil in antimony ore region |
| CN114749479B (en) * | 2022-05-30 | 2023-06-02 | 中南大学 | Method for repairing arsenic-containing gold tailings by utilizing plant-microorganism combination |
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| CN104450552B (en) * | 2014-08-17 | 2017-11-17 | 西北大学 | A kind of sulfate reducing bacteria phosphate solubilizing bacteria and its application in combined repair of cadmium polluted soil |
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