CN109158420B - A kind of As polluted soil renovation agent and application thereof - Google Patents
A kind of As polluted soil renovation agent and application thereof Download PDFInfo
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- CN109158420B CN109158420B CN201810986045.9A CN201810986045A CN109158420B CN 109158420 B CN109158420 B CN 109158420B CN 201810986045 A CN201810986045 A CN 201810986045A CN 109158420 B CN109158420 B CN 109158420B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/02—Extraction using liquids, e.g. washing, leaching, flotation
Abstract
The invention discloses a kind of As polluted soil renovation agent and application thereof, the preparation method of the As polluted soil renovation agent include the activation of P11 bacterial strain, the preparation of fermentation liquid, the preparation of P11 dry powder, As polluted soil renovation agent be made and etc..Bacterial strain and culture medium raw material used in technical solution of the present invention are simple and easy to get, low in cost；Product As polluted soil renovation agent slant acidity, pH are nature pH(6.4 ± 0.2), optimum pH value also for this purpose, without adjusting pH value when using, simplifies technique, secondly the pH value of the very close common soil of the pH value, therefore will not cause big injury to soil；The As polluted soil renovation agent can effectively elute the arsenic in soil, and arsenic totality removal rate is up to 95.52% when dystopy elutes；P11 dry powder is biodegradable as microbial fermentation product, so that the environmental risk of renovation agent recovery technique is reduced, it is small to environmental hazard, it not will cause secondary pollution.
Description
Technical field
The present invention relates to environment remediation technical fields, and in particular to a kind of As polluted soil renovation agent and application thereof.
Background technique
Heavy metal pollution of soil refers to that due to human society practical activity, micro (class) metallic element in soil is in soil
In content be more than certain standard, too high levels caused by excess deposition.Heavy metal in soil source mainly has internal next
Source and two kinds of external source.In house sources, due to soil parent material, topography and geomorphology, hydrometeorology and vegetation and land use
The difference of type etc., the influence to heavy metal content in soil have very big difference, cause some areas soil background higher.It is external
Reason is mainly the influence of human social activity, is the main source of heavy metal pollution of soil, mainly includes the following aspects:
Atmospheric sedimentation, sewage and farming activities, industrial and mining enterprises' production etc..Soil arsenic pollution is mainly derived from arsenic minerals enterprise production and big
Gas sedimentation etc..Heavy metal enters in soil, is not easy to be not easy to be degraded with water leaching, and also there is apparent biological concentration to make
With.
Soil is the material base that the mankind depend on for existence and development, is the important component of natural environment.Not only it is
Object, plant and the mankind provide living space, and mechanical support ability can be provided for plant growth, and provide institute for its growth and development
The fertility element such as water, fertilizer, air and heat for needing.For soil once by arsenic pollution, the toxicity of arsenide can destroy the ecology knot of soil
Structure, or directly affect the healthy and safe of the mankind.On the one hand, soil arsenic pollution destroys soil ecosystem, influences edaphon
Group, soil enzyme activities, the organic duff degradation of soil, soil respiration metabolism etc., to influence edaphon, animal
Growth and development；Also make soil fertility degeneration, influence the growth and development of plant, cause physiological function of plants disorder, trophic disturbance,
Make the equal sharp fall of the yield and quality of crop；Deteriorate water environment.On the other hand, As polluted soil fugitive dust enters to be exposed to and raise
In crowd's body in dirt, or accumulation is migrated with grain or is transmitted by food chain, be enriched with, seriously threaten in people and organism
The health of the mankind.
Arsenic pollution also belongs to heavy metal pollution.Arsenic is a kind of carcinogenic metalloid, and the toxicity of element arsenic is extremely low, and arsenide is equal
Toxic, trivalent arsenic compound (arsenic trioxide is commonly called as arsenic) is more stronger than other arsenic compound toxicity.Reformation and development comes, with
Industrialized development, more and more arsenic minerals exploitations, the industry of arsenic class and the use of the pesticide containing arsenic, makes the accumulation of arsenic in soil
More and more, about 500,000 tons of the arsenic-containing waste residue amount that China generates every year, accumulating untreated arsenic-containing waste residue amount is 2,000,000
Ton, arsenic soil pollution are more and more severeer.2013, Hubei somewhere, the long-term discharge in violation of regulations object containing arsenic pollution of 6 enterprises caused
Some Villagers arsenicalism；Hunan county exploit arsenic disulfide and refining arsenic, make atmosphere, soil, water body arsenic pollution and
It is serious, only just has within 2010 157 villagers to die of cancer, separately has 190 people to suffer from cancer.
In face of the arsenic in soil pollution problem being on the rise, scholars increasingly pay attention to various arsenic in soil pollution amelioration skills
The research and application of art.Currently, common As polluted soil recovery technique specifically includes that physical chemistry reparation, (soil improvement by importing soil from other places is consolidated
Change/stabilisation reparation, heat treating process, electro reclamation, Soil leaching) and biological prosthetic (microorganism remediation, phytoremediation, connection
Close reparation etc.).Soil improvement by importing soil from other places is covered contaminated soil with non-polluting soil or with non-polluting soil displacement contaminated soil, this ruling by law
Significant effect is managed, but longer the time required to recovery soil texture and fertility.The curing/stabilizing reparation of As polluted soil is first will
As (III) is oxidized to As (V), then reacts As (V) with specific complexing agent, is converted into torpescence and nontoxic complex compound, but
Fixed or stabilizer will cause secondary pollution or other injuries to soil.The phytoremediation of phytoremediation, As polluted soil is
Arsenic is extracted using arsenic tolerance or hyperaccumulative plant and its symbiotic microorganism, transfer, absorbs, decompose, conversion or fixed, to reach
The purpose of to removal, reduction or stablizing arsenic compound, or reducing arsenic poison, but arsenic over-accumulated plant (ciliate desert-grass, powder leaf fern etc.)
Usually all biomass is lower, plant is short and small, slow growth.Microorganism remediation, including microorganism to the dissolution of heavy metal, absorption,
The effects of aoxidizing, is fixed, has now been found that the microorganism of more resistance to arsenic and arsenic hyperaccumulation (such as: the arsenic oxygen of pseudomonas
Change bacterium).Elution reparation is repaired to elute with dystopy and be repaired including elution in situ, and elution reparation in situ is primarily referred to as into contaminated soil
Apply elution renovation agent, it made to penetrate through contaminated soil downwards, and with pollutant therein occur desorption, chelating, dissolution or
The physics, chemical action such as complexing, are finally transferred to pollutant in leachates, or stably depositing in the soil；Dystopy elution
It repairs on the basis of mainly eluting reparation in situ, increases an agitating reaction pond (or reactor), then reach processing
The backfill of target soil.
Chemical leaching method carrys out restoration of soil polluted by heavy metal usually using chemical detergent at present, but often will cause secondary
Pollution.In addition, common chelating agent (such as: disodium ethylene diamine tetraacetate (ethylenediaminetetraacetic acid)) is used as a kind of effective chelating agent, to most
Number heavy metal has preferable chelation, but stable chelate can not be formed with anion heavy metal (such as As), and due to
Contain chelating agent (EDTA) in waste water, Stability of Chelate is higher, it is difficult to be handled in a manner of traditional materialization.
Summary of the invention
Present invention aim to address drawbacks described above in the prior art, provide the arsenic dirt of a kind of environment-friendly high-efficiency, low cost
Contaminate soil-repairing agent.
It is a further object of the present invention to provide the purposes of above-mentioned As polluted soil renovation agent.
To achieve the above object, the present invention provides a kind of As polluted soil renovation agents, it is characterised in that preparation method packet
Include following steps:
1) activation of P11 bacterial strain: the P11 bacterial strain is identified as Taiwan pseudomonad (Pseudomonas
Taiwanensis, is preserved in China typical culture collection center, and deposit number is CCTCC No:M 2016700)；
2) preparation of fermentation liquid: the strain after the activation obtained to step 1) ferments, and obtains fermentation liquid；
3) preparation of P11 dry powder: P11 dry powder is prepared with the fermentation liquid that step 2) obtains；
4) As polluted soil renovation agent is obtained: the P11 dry composition obtained with step 3) obtains the As polluted soil
Renovation agent.
Preferably, the activation method of bacterial strain described in step 1) are as follows: first by the Taiwan pseudomonad of preservation
(Pseudomonas taiwanensis) strain thaws under ice bath, when the Taiwan pseudomonad of the preservation saves, bacterium solution
OD600Between 0.6-0.7, the glycerol that bacterium solution and concentration of volume percent are 50% is quick-frozen under liquid nitrogen in equal volume than mixing,
- 70 DEG C of refrigerators are gone to again to save；Then it is inoculated with TSB culture of the strain in 100 times of volumes of the defrosting in superclean bench
In base, it is placed on constant-temperature shaking incubator shaken cultivation under the conditions of 180rpm, 30 DEG C and for 24 hours, obtains the bacterium solution of activation, the TSB training
It must be through the 20min that sterilizes under the conditions of steam pressure sterilization pot 0.1Mpa, 121 DEG C when feeding basigamy.
It is further preferred that fermentation liquid described in step 2) the preparation method comprises the following steps: being inoculated with the bacterium solution of the activation in being equipped with
In the fermentor of activation 20 times of volume TSB culture mediums of bacterium solution, trained under the conditions of 30 DEG C, the ventilatory capacity of 180r/min and 1vvm
It supports for 24 hours to get the fermentation liquid.
It is further preferred that P11 dry powder described in step 3) the preparation method comprises the following steps: by the fermentation liquid directly air inlet temperature
It is spray-dried under conditions of 170 DEG C, 75 DEG C of leaving air temp of degree, it is then dry in 37 DEG C of air dry oven, until dry
The mass conservation of dry object obtains P11 dry powder to get described.
It is further preferred that the obtained method of As polluted soil renovation agent described in step 4) are as follows: dissolve institute with ultrapure water
P11 dry powder is stated, so that the P11 dry powder concentration is 50g/L to get the As polluted soil renovation agent.
The present invention also provides purposes of the As polluted soil renovation agent in As polluted soil reparation.
Preferably, purposes of the As polluted soil renovation agent in As polluted soil reparation, it is characterised in that operation
Method are as follows: excavate As polluted soil, through broken and sorting, be sent to and be stirred to react container；By ground moistening, hold to being stirred to react
The As polluted soil renovation agent is added in device, is mixed；Liquid will be mixed and be transferred to solid-liquid separation tank, stand, pass through sand
Net, gauze or the mode for helping membrane filtration, make leachates flow into leachates collecting pit；Chemical precipitation method, electricity are used to leachates
Solution, physical method or biological method processing, discharge after keeping leachates processing up to standard；Wherein, by the method for ground moistening be to
The pure water moist soil 5min that volume in liters are several 2 times of quality of soil sample kilogram is added in soil, to being stirred to react in container
When the As polluted soil renovation agent is added, the volume in liters for the As polluted soil renovation agent being added are quality of soil sample kilogram numbers
5 times, the time of mixing is 20h, and it is 1h that liquid, which is mixed, and is transferred to the time stood in solid-liquid separation tank, uses filtered through gauze
3 layers of gauze of Shi Caiyong.
Arsenic oxidizing bacteria Taiwan pseudomonad (the Pseudomonas taiwanensis that present invention As polluted soil separates
11, i.e., the described P11 bacterial strain) fermentation liquid carry out being spray-dried dry powder obtained (the i.e. described P11 dry powder) arsenic pollution be made
Soil-repairing agent.
Technical solution of the present invention at least has following the utility model has the advantages that used bacterial strain and culture medium raw material are simple easy
, it is low in cost；Product As polluted soil renovation agent slant acidity, pH be nature pH (6.4 ± 0.2), optimum pH value also for this purpose,
Do not have to adjust pH value when use, simplify technique, secondly the pH value of the very close common soil of the pH value, therefore will not be to soil
Cause big injury；The As polluted soil renovation agent can effectively elute the arsenic in soil, and arsenic totally removes when dystopy elutes
Rate is up to 95.52%；P11 dry powder is biodegradable as microbial fermentation product, to reduce renovation agent recovery technique
Environmental risk, it is small to environmental hazard, it not will cause secondary pollution.
Detailed description of the invention
Fig. 1 is that bacteria used thereby Pseudomonas taiwanensis microscope (1000X) of the present invention observes photo figure；
Fig. 2 is bacteria used thereby Pseudomonas taiwanensis flat-plate bacterial colony aspect graph of the present invention；
Fig. 3 is evolution tree graph；
Fig. 4 is that As polluted soil of the present invention elutes repair process flow.
Specific embodiment
The detection of the bacteria used thereby Pseudomonas taiwanensis of the present invention of embodiment 1
(1) form
Bacteria used thereby Pseudomonas taiwanensis microscope (1000X) observation photo of the present invention is shown in Fig. 1, plate bacterium
It falls aspect graph and sees Fig. 2.
(2) 16S rRNA gene sequencing
1. 16S rRNA gene order
Sequence table
2. 16S rRNA gene sequencing BLAST result
16S rRNA gene sequencing BLAST the results are shown in Table 1.
The bacteria used thereby Pseudomonas taiwanensis 16S rRNA gene sequencing BLAST result of the present invention of table 1
3. chadogram
Chadogram sees Fig. 3.
(3) bacteria used thereby Pseudomonas taiwanensis physio-biochemical characteristics of the present invention
Bacteria used thereby Pseudomonas taiwanensis physio-biochemical characteristics testing result of the present invention is shown in Table 2, table 3, as a result
For Gram-negative bacteria.
2 bacterial strain physio-biochemical characteristics of table-enzyme activity, carbon source oxidation
+: positive reaction；: negative reaction；
3 bacterial strain physio-biochemical characteristics of table-produce acid using carbon source
+: positive reaction；: negative reaction；W: weakly positive reaction
The As polluted soil renovation agent of the present invention of embodiment 2 elutes laboratory soil
1) the specific method is as follows for the preparation of P11 dry powder:
A. it prepares culture medium: preparing pancreas peptone soybean broth (TSB) culture medium, steam pressure sterilization pot 0.1Mpa, 121
Sterilize 20min under the conditions of DEG C；
B. thaw strain: when P11 fungi preservation, the OD of bacterium solution600Between 0.6-0.7, bacterium solution and concentration of volume percent
For 50% glycerol in equal volume than mixing, quick-frozen under liquid nitrogen, then go to -70 DEG C of refrigerators and save, the strain of preservation is under ice bath
It thaws；
C. activated spawn: being inoculated with the strain of above-mentioned defrosting in superclean bench, (strain and TSB are trained in TSB culture medium
The volume ratio for supporting base is 1:100), constant-temperature shaking incubator is placed under the conditions of 180rpm, 30 DEG C, and shaken cultivation is about for 24 hours；
D. inoculation fermentation: it is inoculated with the bacterium solution of the activation (bacterium solution and TSB liquid of activation in the fermentor equipped with TSB culture medium
The volume ratio of body culture medium is 5:100), it is cultivated for 24 hours under the conditions of 30 DEG C, the ventilatory capacity of 180r/min and 1vvm；
E. prepare dry powder: fermentation liquid is directly done under conditions of 170 DEG C of inlet air temperature, 75 DEG C of leaving air temp by spraying
It is dry, it is then dry in 37 DEG C of air dry oven, until the mass conservation of dried object does P11 so that P11 dry powder be made
Powder is placed to be saved backup at dry；
2) the P11 dry powder solution that 50g/L is prepared with ultrapure water, to elute renovation agent as dystopy；
3) heavy-metal contaminated soil (content of beary metal Cd:0.975mg/kg is prepared；Pb:312.792mg/kg；As:
17.301mg/kg), be then placed in keg, depth of soil about 25cm keeps the humidity of soil, when keg surface soil because
When small crack or comparison dry soil dust and particle occurs in arid, ultrapure water is added, until surface just is diffused out, placement 4 naturally
A month or more；
4) above-mentioned soil sample natural air drying removes impurity removing, crosses 2mm aperture sieve, takes 2g in the centrifuge tube of 15mL, 4mL is added
Pure water, moist soil 5min adds 10mL renovation agent, the stirring elution 20h at 180rpm, 37 DEG C, centrifuge separation soil
Earth and leachates measure arsenic and lead concentration in leachates, calculate arsenic and lead content and the removal rate (removal of heavy metal of leachates
Content of beary metal × 100% in rate=leachates content of beary metal ÷ elution soil sample), arsenic and lead concentration, content in leachates
And removal rate is shown in Table 4.
The concentration of arsenic and lead, content and removal rate in 4 leachates of table
5) after single wash, in 2g soil sample (arsenic content: 34.6 μ g), the average elution amount of As is the flat of 15.067 μ g, As
Equal removal rate can reach 43.55%.
The As polluted soil renovation agent of the present invention of embodiment 3 elutes the topsoil of certain industrial park
Technique is eluted according to shown in Fig. 4.
1) P11 dry powder (preparation method is with embodiment 2) solution containing 50g/L is prepared with ultrapure water, to repair as elution
Agent；
2) topsoil of certain industrial park heavy metal pollution region 0-35cm is acquired, soil sample natural air drying goes impurity removing, mistake
2mm aperture sieve, packing save backup；
3) soil acid is cleared up: weighing 2g soil sample in the conical flask of 100mL, adding 600 μ L ddH2O moist soil is stood
After 10min, be sequentially added into 5mL concentrated nitric acid (mass fraction 65%-68%), the 3mL concentrated sulfuric acid (mass fraction 49%),
1mL perchloric acid (mass fraction 70%-72%) stands 2.5h, is again heated to boiling, after boiling emits white cigarette, often shakes, directly
To becoming canescence, then cool down；Then 5mL concentrated nitric acid (mass fraction 65%-68%) and 10mL ddH is added2O, heating boiling
It rises, until solution is evaporated to remaining 5mL；When being finally cooled to conical flask non-scald on hand (about 37 DEG C), 15mL ddH is added2O is abundant
Soil is dissolved, then is filtered with neutral filter paper；Then it is added three times 5mL ddH2O washs soil, then is filtered with neutral filter paper, so
50mL, which is settled to, with graduated cylinder afterwards (uses ddH2O constant volume), detect total metals (content of beary metal Zn:1915mg/ in soil sample
kg；As:457.5mg/kg)；
4) take 2g soil sample in the beaker of 50mL, being added the pure water of 4mL, moist soil 5min adds 10mL reparation
Agent is collected leachates, is measured in leachates after stirring elution 20h, static 1h under magnetic stirring apparatus by 3 layers of filtered through gauze
Arsenic (As) concentration, calculates the arsenic content and removal rate of leachates, and arsenic concentration, content and removal rate are shown in Table 5 in leachates.
Concentration, content and the removal rate of arsenic in 5 leachates of table
5) after single wash, in 2g soil sample (arsenic content: 915 μ g), the removal rate that the elution amount of As is 874 μ g, As is reachable
To 95.52%.
The As polluted soil renovation agent of the present invention of embodiment 4 elutes the industrial residue of certain industrial park
Technique is eluted according to shown in Fig. 4.
1) P11 dry powder (preparation method is with embodiment 2) solution containing 50g/L is prepared with ultrapure water, to repair as elution
Agent；
2) industrial residue of certain industrial park heavy metal pollution is acquired, natural air drying removes impurity removing, 2mm aperture sieve is crossed, point
Dress saves backup；
3) industrial residue acid is cleared up: weighing 2g industrial residue in the conical flask of 100mL, adding 600 μ L ddH2O humid soil
Earth is sequentially added into 5mL concentrated nitric acid (mass fraction 65%-68%), the 3mL concentrated sulfuric acid (mass fraction after standing 10min
For 49%), 1mL perchloric acid (mass fraction 70%-72%), stand 2.5h, be again heated to boiling, after boiling emits white cigarette, pass through
It often shakes, until becoming canescence, then cools down；Then 5mL concentrated nitric acid (mass fraction 65%-68%) and 10mL is added
ddH2O, ebuillition of heated, until solution is evaporated to remaining 5mL；When being finally cooled to conical flask non-scald on hand (about 37 DEG C), it is added
15mL ddH2O sufficiently dissolves industrial residue, then is filtered with neutral filter paper；Then it is added three times 5mL ddH2O detergent industry is useless
Slag, then filtered with neutral filter paper, 50mL then, which is settled to, with graduated cylinder (uses ddH2O constant volume), it is total to detect heavy metal in industrial residue
Measure (content of beary metal Zn:19260mg/kg；Pb:984mg/kg；As:86.6mg/kg；Co:109.6mg/kg)；
4) industrial residue for taking 2g spare is in the beaker of 50mL, being added the pure water of 4mL, moist soil 5min, then plus
Enter 10mL renovation agent, collects leachates by 3 layers of filtered through gauze after stirring elution 20h, static 1h under magnetic stirring apparatus, survey
Determine heavy metal concentration in leachates, calculate the content of beary metal and removal rate of leachates, heavy metal concentration in leachates, content and
Removal rate is shown in Table 6.
Heavy metal concentration, content and removal rate in 6 leachates of table
5) after single wash, (content of arsenic and zinc difference: 915 μ g and 38520 μ g), the elution amount of As in 2g industrial residue
Removal rate for 34.6 μ g, As is 19.98%, and secondly the single elution amount of zinc can reach 3490 μ g.
Sequence table
<110>Hubei normal university
<120>a kind of As polluted soil renovation agent and application thereof
<141> 2018-08-27
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1432
<212> DNA
<213>Taiwan pseudomonad (Pseudomonas taiwanensis)
<400> 1
tggcggcagc tacacatgca gtcgagcgga tgacgggagc ttgctccttg attcagcggc 60
ggacgggtga gtaatgccta ggaatctgcc tggtagtggg ggacaacgtt tcgaaaggaa 120
cgctaatacc gcatacgtcc tacgggagaa agcaggggac cttcgggcct tgcgctatca 180
gatgagccta ggtcggatta gctagttggt ggggtaatgg ctcaccaagg cgacgatccg 240
taactggtct gagaggatga tcagtcacac tggaactgag acacggtcca gactcctacg 300
ggaggcagca gtggggaata ttggacaatg ggcgaaagcc tgatccagcc atgccgcgtg 360
tgtgaagaag gtcttcggat tgtaaagcac tttaagttgg gaggaagggc agtaagttaa 420
taccttgctg ttttgacgtt accgacagaa taagcaccgg ctaactctgt gccagcagcc 480
gcggtaatac agagggtgca agcgttaatc ggaattactg ggcgtaaagc gcgcgtaggt 540
ggtttgttaa gttggatgtg aaagccccgg gctcaacctg ggaactgcat ccaaaactgg 600
caagctagag tacggtagag ggtggtggaa ttttctgtgt agcggtgaaa tgcgtagata 660
taggaaggaa caccagtggc gaaggcgacc acttggactg atactgacac tgaggtgcga 720
aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa cgatgtcaac 780
tagccgttgg aatccttgag attttagtgg cgcagctaac gcattaagtt gaccgcctgg 840
ggagtacggc cgcaaggtta aaactcaaat gaattgacgg gggcccgcac aagcggtgga 900
gcatgtggtt taattcgaag caacgcgaag aaccttacca ggccttgaca tgcagagaac 960
tttccagaga tggattggtg ccttcgggaa ctctgacaca ggtgctgcat ggctgtcgtc 1020
agctcgtgtc gtgagatgtt gggttaagtc ccgtaacgag cgcaaccctt gtccttagtt 1080
accagcacgt tatggtgggc actctaagga gactgccggt gacaaaccgg aggaaggtgg 1140
ggatgacgtc aagtcatcat ggcccttacg gcctgggcta cacacgtgct acaatggtcg 1200
gtacagaggg ttgccaagcc gcgaggtgga gctaatctca caaaaccgat cgtagtccgg 1260
atcgcagtct gcaactcgac tgcgtgaagt cggaatcgct agtaatcgcg aatcagaatg 1320
tcgcggtgaa tacgttcccg ggccttgtac acaccgcccg tcacaccatg ggagtgggtt 1380
gcaccagaag tagctagtct aaccttcggg aggacggtac cacggtgatc gt 1432
Claims (2)
1. a kind of As polluted soil renovation agent, it is characterised in that preparation method includes the following steps:
1) activation of P11 bacterial strain: the P11 bacterial strain is identified as Taiwan pseudomonad (Pseudomonas taiwanensis),
It is preserved in China typical culture collection center, deposit number is CCTCC No:M 2016700；
2) preparation of fermentation liquid: the strain after the activation obtained to step 1) ferments, and obtains fermentation liquid；
3) preparation of P11 dry powder: P11 dry powder is prepared with the fermentation liquid that step 2 obtains；
4) As polluted soil renovation agent is obtained: the P11 dry composition obtained with step 3) obtains the As polluted soil reparation
Agent；
Wherein, the activation method of bacterial strain described in step 1) are as follows: first by the Taiwan pseudomonad (Pseudomonas of preservation
Taiwanensis) strain thaws under ice bath, when the Taiwan pseudomonad of the preservation saves, the OD of bacterium solution600In 0.6-0.7
Between, the glycerol that bacterium solution and concentration of volume percent are 30% is quick-frozen under liquid nitrogen in equal volume than mixing, then goes to -70 DEG C of refrigerators
It saves；Then it is inoculated with the strain of the defrosting in superclean bench in the TSB culture medium of 100 times of volumes, is placed on constant temperature vibration
Incubator 24 h of shaken cultivation under the conditions of 180 rpm, 30 DEG C is swung, the bacterium solution of activation, palpus when the TSB culture medium is prepared are obtained
Through 20 min that sterilize under the conditions of 0.1 Mpa of steam pressure sterilization pot, 121 DEG C；
Fermentation liquid described in step 2 the preparation method comprises the following steps: being inoculated with the bacterium solution of the activation in equipped with described 20 times of bodies of activation bacterium solution
In the fermentor of product TSB culture medium, 24 h are cultivated to get described under the conditions of 30 DEG C, the ventilatory capacity of 180 r/min and 1 vvm
Fermentation liquid；
P11 dry powder described in step 3) the preparation method comprises the following steps: by the fermentation liquid directly in 170 DEG C of inlet air temperature, leaving air temp
It is spray-dried under conditions of 75 DEG C, it is then dry in 37 DEG C of air dry oven, until the mass conservation of dried object, i.e.,
P11 dry powder is obtained described in obtaining；
The obtained method of As polluted soil renovation agent described in step 4) are as follows: the P11 dry powder is dissolved with ultrapure water, so that described
P11 dry powder concentration is 50g/L to get the As polluted soil renovation agent.
2. a kind of purposes of As polluted soil renovation agent as described in claim 1 in As polluted soil reparation, feature exist
In operating method are as follows: excavate As polluted soil, through broken and sorting, be sent to and be stirred to react container；By ground moistening, to stirring
The As polluted soil renovation agent is added in reaction vessel, is mixed；Liquid will be mixed and be transferred to solid-liquid separation tank, stand,
By way of sand net, gauze or helping membrane filtration, leachates is made to flow into leachates collecting pit；Chemical precipitation is used to leachates
Method, electrolysis method, physical method or biological method processing, discharge after keeping leachates processing up to standard；
It wherein, is that addition volume in liters are 2 times several of pure water of quality of soil sample kilogram into soil by the method for ground moistening
It is wet
Moisten soil 5min, to be stirred to react in container the As polluted soil renovation agent is added when, the As polluted soil that is added
The volume in liters of renovation agent are several 5 times of quality of soil sample kilogram, and the time of mixing is 20h, and liquid is mixed and is transferred to solid-liquid
The time stood in separate tank is 1h, with when filtered through gauze use 3 layers of gauze.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810986045.9A CN109158420B (en) | 2018-08-27 | 2018-08-27 | A kind of As polluted soil renovation agent and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810986045.9A CN109158420B (en) | 2018-08-27 | 2018-08-27 | A kind of As polluted soil renovation agent and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109158420A CN109158420A (en) | 2019-01-08 |
| CN109158420B true CN109158420B (en) | 2019-10-18 |
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| CN109772867B (en) * | 2019-02-22 | 2021-09-24 | 芷兰生态环境建设有限公司 | Heavy metal polluted soil treatment system and treatment method |
| CN109940041A (en) * | 2019-03-27 | 2019-06-28 | 徐州工程学院 | A kind of serious pollution soil restoring device |
| CN111234835B (en) * | 2020-02-17 | 2021-04-30 | 兰州大学 | Repairing agent for in-situ repairing heavy metal contaminated soil and in-situ repairing method |
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| CN113424750A (en) * | 2021-06-10 | 2021-09-24 | 湖北师范大学 | Planting method for reducing cadmium content of rice |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104004691A (en) * | 2014-06-16 | 2014-08-27 | 中南大学 | Bacterial strain used for arsenic polluted soil remediation and application method thereof |
| CN105234168A (en) * | 2015-11-20 | 2016-01-13 | 福建康德贝生物科技有限公司 | Application of bacillus subtilis |
| CN107052038A (en) * | 2017-05-12 | 2017-08-18 | 中国农业科学院农业环境与可持续发展研究所 | A kind of method that utilization chemistry of micro-organisms method repairs As polluted soil |
-
2018
- 2018-08-27 CN CN201810986045.9A patent/CN109158420B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104004691A (en) * | 2014-06-16 | 2014-08-27 | 中南大学 | Bacterial strain used for arsenic polluted soil remediation and application method thereof |
| CN105234168A (en) * | 2015-11-20 | 2016-01-13 | 福建康德贝生物科技有限公司 | Application of bacillus subtilis |
| CN107052038A (en) * | 2017-05-12 | 2017-08-18 | 中国农业科学院农业环境与可持续发展研究所 | A kind of method that utilization chemistry of micro-organisms method repairs As polluted soil |
Non-Patent Citations (1)
| Title |
|---|
| 《食品微生物学实验》;梁志宏;《食品微生物学实验》;20131231;第54页 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113403226B (en) * | 2021-06-04 | 2022-05-31 | 山西大学 | Heavy metal tolerant microbial strain and application thereof |
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