CN101921709A - Composite high-efficiency microorganism preparation for synergy of sewage treatment plant and synergistic process - Google Patents

Composite high-efficiency microorganism preparation for synergy of sewage treatment plant and synergistic process Download PDF

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CN101921709A
CN101921709A CN2010102496130A CN201010249613A CN101921709A CN 101921709 A CN101921709 A CN 101921709A CN 2010102496130 A CN2010102496130 A CN 2010102496130A CN 201010249613 A CN201010249613 A CN 201010249613A CN 101921709 A CN101921709 A CN 101921709A
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culture
nutrient solution
sewage
microorganism preparation
highly effective
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CN101921709B (en
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李沧海
付建军
黄海
魏雪松
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Santai Zhengfang Biological Environment Sci & Tech Development Co Ltd Beij
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Santai Zhengfang Biological Environment Sci & Tech Development Co Ltd Beij
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Abstract

The invention discloses a composite high-efficiency microorganism preparation for synergy of a sewage treatment plant and a synergistic process. Active ingredients of the composite high-efficiency microorganism preparation comprise zoogloea, comamonas, bacillus, pseudomonas, alcaligenes and nocardia. A method for preparing the composite high-efficiency microorganism preparation comprises the following steps of: independently cultivating the zoogloea, the comamonas, the bacillus, the pseudomonas, the alcaligenes and the nocardia in culture media respectively to obtain a zoogloea culture, a comamonas culture, a bacillus culture, a pseudomonas culture, an alcaligenes culture and a nocardia culture; and mixing the cultures. When the composite high-efficiency microorganism preparation is applied to the synergy of the sewage treatment plant, the composite high-efficiency microorganism preparation is added into a biochemical tank of the sewage treatment plant after expanding culture and acclimatization, so the biomass and activity are improved, treatment capacity and treatment effect of a sewage treatment system are improved, and standard emission and stable operation of the system can be guaranteed.

Description

The composite and highly effective microorganism preparation and the synergistic process that are used for synergy of sewage treatment plant
Technical field
The present invention relates to a kind of composite and highly effective microorganism preparation and synergistic process that is used for synergy of sewage treatment plant.
Background technology
Dirty water living creature processing technique has become the dominant technology of current sewage treatment area, and it is improving the water body environment quality, preventing to play an important role aspect the water pollution as a kind of economical rationality, effective sewage disposal technology.
The biological treatment of sewage is the metabolism that utilizes microorganism, and the decomposition and inversion pollutants in sewage reaches the purpose that purifies water.
The core of biological sewage treatment is to cultivate to tame out the superior microorganism flora that is fit to the sewage characteristics, and maintenance and sewage pollution the substrate concentration microbial biomass and the activity that adapt, the superior microorganism flora is handled in best degrading activity, give full play to its degradation function, improve the biological treatment ability and the processing efficiency of system to greatest extent.
Biological sewage treatment generally adopts nature culturing sludge acclimated microorganism at present, though technical maturity, but there is the shortcoming that is difficult to overcome, be mainly reflected in: bad adaptability, hazardous and noxious substances and supersalinity can suppress microbial growth even cause microorganism deactivatedly in the sewage, and most of industrial sewages especially high-concentration difficult-degradation organic sewage and high-ammonia-nitrogen sewage are difficult to handle; Culture cycle is long, at Sewage Plant easy inactivation of microorganism between turn(a)round, sludge bulking occurs, must cultivate again when sewage work moves once more; Biomass is few, and degradation capability is poor, and processing efficiency is low, and macromole, difficult degradation, poisonous and hazardous organism and nitrogen, phosphorus etc. are difficult to effectively, thoroughly degrade, and goes out water unstable; To the poor processing effect of ammonia nitrogen, and when ammonia nitrogen concentration is high, can inhibition be arranged, thereby reduce processing efficiency microbic activity; Shock resistance is poor, and amount of inlet water, concentration, temperature, PH etc. are required height, operational administrative complexity; Fluctuation of service is difficult to guarantee that sewage work is normal, steady running and qualified discharge.So utilize nature culturing sludge acclimated microorganism that certain limitation is arranged, do not reach the optimum handling effect, especially a large amount of dischargings of industrial sewage and improving constantly of state sewage emission standard, natural culturing sludge acclimated microorganism has been difficult to satisfy the new demand of biological sewage treatment.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of composite and highly effective microorganism preparation and synergistic process that is used for synergy of sewage treatment plant.
Provided by the invention be used for synergy of sewage treatment plant the activeconstituents of composite and highly effective microorganism preparation comprise: moving glue bacterium, comamonas, genus bacillus, pseudomonas, Alcaligenes and Nocardia.
Preferably, above-mentioned composite and highly effective microorganism preparation comprises the component of following parts by weight:
Moving glue bacterium culture 25-30 part,
Comamonas culture 20-25 part,
Bacillus culture 15-20 part,
Pseudomonas culture 15-20 part,
Alcaligenes culture 5-10 part,
Nocardia culture 5-10 part.
The invention provides the preparation method of above-mentioned composite and highly effective microorganism preparation: will move the glue bacterium, comamonas, genus bacillus, pseudomonas, Alcaligenes and Nocardia be single culture in substratum respectively, obtain moving glue bacterium culture, comamonas culture, bacillus culture, pseudomonas culture, Alcaligenes culture and Nocardia culture, mix.
As preferably, aforesaid method comprises the steps:
1) will move the glue bacterium, comamonas, genus bacillus, pseudomonas, Alcaligenes and Nocardia be enlarged culturing respectively, obtains the bacterium liquid of enlarged culturing;
2) be that the bacterium liquid of the enlarged culturing of substratum 5-10% inserts respectively and carries out independent fermentation culture in the substratum with weight, obtain moving glue bacterium culture, comamonas culture, bacillus culture, pseudomonas culture, Alcaligenes culture and Nocardia culture.
Optional solid medium of above-mentioned substratum or liquid nutrient medium.
Above-mentioned composite and highly effective microorganism preparation is used for the technology of synergy of sewage treatment plant, comprises the steps:
1) clear water and treatment sewage are mixed, add glucose, peptone, nitrogen-phosphorus-potassium compound fertilizer and trace element water-soluble fertilizer, regulate PH to 7-8, add described composite and highly effective microorganism preparation, aeration or stirring 24-48 hour, make nutrient solution 1, in the nutrient solution 1 input sewage biochemical treatment tank with volume 80~90%, be used for sewage disposal.
2) in remaining nutrient solution 1, add clear water and treatment sewage, add glucose, peptone, nitrogen-phosphorus-potassium compound fertilizer and trace element water-soluble fertilizer, regulate PH to 7~8, add described composite and highly effective microorganism preparation, aeration or stirring 24-48 hour, make nutrient solution 2, in the nutrient solution 2 input sewage biochemical treatment tanks with volume 80~90%, be used for sewage disposal.
3) get step 2) remaining nutrient solution 2, operation is with step 2), make nutrient solution 3, in the nutrient solution 3 input sewage biochemical treatment tanks with volume 80~90%, be used for sewage disposal.
4) get the remaining nutrient solution 3 of step 3), operation is with step 2), make nutrient solution 4, in the nutrient solution 4 input sewage biochemical treatment tanks with volume 80~90%, be used for sewage disposal.
5) get the remaining nutrient solution 4 of step 4), operation is with step 2), make nutrient solution 5, nutrient solution 5 is all dropped in the sewage biochemical treatment tank, be used for sewage disposal.
As preferably, contain trace elements iron, copper, manganese, zinc, boron and molybdenum in the above-mentioned trace element water-soluble fertilizer.
Aforesaid method also comprises: repeating step 1)~and the operation of step 5) 1~5 time.
As preferably, the weight ratio of the add-on of clear water described in the step 1), treatment sewage, glucose, peptone, nitrogen-phosphorus-potassium compound fertilizer, trace element water-soluble fertilizer and composite and highly effective microorganism preparation is: 50: 50: 1~2: 0.3~0.5: 0.2~0.4: 0.1~0.3: 8~10; Step 2)~step 5) described in the remaining nutrient solution 1~4 any one, the weight ratio of clear water, treatment sewage, glucose, peptone, nitrogen-phosphorus-potassium compound fertilizer, trace element water-soluble fertilizer and composite and highly effective microorganism preparation add-on is: 10~20: 40~45: 40~45: 1~2: 0.3~0.5: 0.2~0.4: 0.1~0.3: 4~5.
As preferably, in the above-mentioned nutrient solution 1~5 any one dosage that drops into the sewage biochemical treatment tank be in the described sewage biochemical treatment tank sewage weight 0.1~1%.
The present invention can reach following technique effect:
The present invention is according to the characteristics of sewage work's sewage, to composite and highly effective microorganism preparation expand numerous, cultivate, domestication, can cultivate at short notice and tame out the high-effective microorganism dominant microflora that is fit to the sewage characteristics, improve the microorganism active and the quantity of sewage disposal greatly, improve biodegradability and processing efficiency, improve wastewater treatment efficiency, shorten and cultivate the domestication time, use simply, easy to operate, can guarantee system stable operation.
The present invention is applicable to sewage work's high efficient microbe synergistic, both be applicable to the processing of municipal sanitary sewage, various industrial sewages, also be applicable to the improvement that the rivers and lakes environment water pollutes, especially to the reluctant high density of traditional activated sludge process, be difficult to resolve fall, poisonous and harmful and high saliniferous organic sewage and high-ammonia-nitrogen sewage have unique treatment effect.
The synergistic process that composite and highly effective microorganism preparation provided by the invention is used for sewage work has the following advantages:
(1) the microorganism reproductive speed is fast, cultivates domestication time weak point, can go out a large amount of dominant microfloras by fast breeding;
(2) cultured microorganism adaptability is strong, and difficult degradation, macromole, hazardous and noxious substances and ammonia nitrogen are had good adaptability and degradation effect;
(3) strong shock resistance when Sewage treatment systems is subjected to impacting (discharge of sewage increases suddenly or concentration of wastewater increases suddenly) or restarts, can start fast with recovery system and move;
(4) consumption of saving microorganism improves the microorganism utilising efficiency;
(5) can reduce input cost, have remarkable economic efficiency and social benefit;
(6) use is simple, easy to operate, and instant effect can be received good wastewater treatment efficiency in 5-10 days;
(7) strengthening synergy by high-effective microorganism and can improve the processing power and the treatment effect of sewage work, is the effective ways that new Sewage treatment systems starts fast and existing old sewage work is carried out dilatation and technological transformation.
Embodiment
The invention will be further described below in conjunction with specific embodiment, can be implemented so that those skilled in the art can better understand the present invention also, but illustrated embodiment is not as a limitation of the invention.
Embodiment 1: the enforcement the present invention of sewage work who to the biochemistry pool useful volume is 360 cubic metres.
Sewage work's day is handled water yield 480m 3/ d, treatment process are A/O, and the biochemistry pool useful volume is 360 cubic metres, and hydraulic detention time is 18 hours, about water inlet COD mean value 4000mg/L, about influent ammonia nitrogen mean value 180mg/L.Concrete steps are as follows:
1, the cultivation of bacterium: will move glue bacterium, comamonas, genus bacillus, pseudomonas, Alcaligenes and Nocardia, carry out the seed enlarged culturing respectively, get the bacterium liquid of enlarged culturing, be that the bacterium liquid of the enlarged culturing of substratum 5-10% inserts respectively in the substratum of fermentor tank and carries out independent fermentation culture with weight, leavening temperature keeps 30-40 ℃, fermentation time 48-72 hour, the air oxygen supply after fermenting process adopts mechanical stirring or feeds filtration.After the fermentation culture, obtain moving glue bacterium culture, comamonas culture, bacillus culture, pseudomonas culture, Alcaligenes culture and Nocardia culture.Bacteria content is in the culture of single Pseudomonas: hundred million/gram of 10-50.
Wherein, substratum is prepared in following ratio: soyflour 8-12g, Semen Maydis powder 8-12g, glucose 10-20g, sodium-chlor 3-8g, lime carbonate 1-3g, potassium primary phosphate 150-200mg, ferrous sulfate 25-35mg, manganous sulfate 20-25mg, zinc sulfate 5-10mg, water 1000mL.
2, the culture that step 1 is obtained is pressed column weight amount umber and is carried out composite: 25 parts of moving glue bacterium cultures, 20 parts of comamonas cultures, 15 parts of bacillus cultures, 20 parts of pseudomonas cultures, 10 parts of Alcaligenes cultures, 10 parts of Nocardia cultures.Make the high-effective microorganism compound formulation.
3, the compound system of the high-effective microorganism that makes is enlarged domestication and cultivate, and will tame cultured microorganism bacterium liquid and add in Sewage treatment systems, specifically implementation step is as follows:
The 1st step: select a useful volume greater than 2 cubic metres container, the clear water (weighing 1000 kilograms) that in container, adds 1000 liters, 1000 liters of pending sewage (weighing 1000 kilograms), mix the back and add 20 kilograms of glucose, 6 kilograms of peptones, 4 kilograms of nitrogen-phosphorus-potassium compound fertilizers, 2 kilograms of strong Feng You trace element fertilizer (its iron content, copper, manganese, zinc, trace element such as boron and molybdenum, No. 159, the fertile accurate word of farming, available from the safe legendary god of farming's scientific and technological development in Beijing three company limited), regulate PH to 7-8, add 200 kilograms of high-effective microorganism microbial inoculums, aeration or stirred 24 hours, make nutrient solution 1, nutrient solution 1 with volume 90%, promptly 1800 liters, evenly throw and be spread across in each sewage biochemical treatment tank, stay 10% nutrient solution 1 promptly 200 kilograms make Inoculant;
The 2nd step: in remaining 10% nutrient solution 1, promptly make Inoculant for 200 liters, add 900 liters clear water, 900 liters of pending sewage, mix the back and add 20 kilograms of glucose, 6 kilograms of peptones, 4 kilograms of nitrogen-phosphorus-potassium compound fertilizers, 2 kilograms of strong Feng You trace element fertilizer (trace elements such as its iron content, copper, manganese, zinc, boron and molybdenum, No. 159, the fertile accurate word of farming, available from the safe legendary god of farming's scientific and technological development in Beijing three company limited), regulate PH to 7-8; Add 100 kilograms of high-effective microorganism microbial inoculums, aeration or stir 24 hours after, nutrient solution 2, with the nutrient solution 2 of volume 90%, promptly 1800 liters, evenly throw and be spread across in the sewage biochemical treatment tank, stay 10% nutrient solution 2 to make Inoculant;
The 3rd step: in remaining 10% nutrient solution 2, promptly make Inoculant for 200 liters, all the other steps make nutrient solution 3 with the 2nd step, and with the nutrient solution 3 of volume 90%, promptly 1800 liters, evenly throwing is spread across in the sewage biochemical treatment tank, stays 10% nutrient solution 3 to make Inoculant;
The 4th step: in remaining 10% nutrient solution 3, promptly make Inoculant for 200 liters, all the other steps make nutrient solution 4 with the 2nd step, and with the nutrient solution 4 of volume 90%, promptly 1800 liters, evenly throwing is spread across in the sewage biochemical treatment tank, stays 10% nutrient solution 4 to make Inoculant;
The 5th step: in remaining 10% nutrient solution 4, promptly make Inoculant for 200 liters, all the other steps make nutrient solution 5 with the 2nd step, nutrient solution 5 are all thrown be spread across in the sewage biochemical treatment tank.
Repeating the 1st~5 goes on foot 5 times.
Before not using technical scheme of the present invention, about this sewage disposal plant effluent COD mean value 2000mg/L, clearance is about 50%, and ammonia nitrogen is not almost had removal effect.Implement after the present invention, average effluent COD is less than 100mg/L, clearance 97.5%; Water outlet ammonia ammonia mean value is less than 8mg/L, and clearance is more than 95%.The qualified discharge requirement is satisfied in water outlet.
Embodiment 2: the enforcement the present invention of sewage work who to the biochemistry pool useful volume is 5400 cubic metres.
Sewage work's day is handled water yield 2880m 3/ d, biochemical processing process are UASB+SBR, and the biochemistry pool useful volume is 5400 cubic metres, and hydraulic detention time is 45 hours, about UASB water inlet COD mean value 8000mg/L, about influent ammonia nitrogen mean value 300mg/L.Concrete steps are as follows:
1, the cultivation of bacterium: will be from active sludge isolating moving glue bacterium, comamonas, genus bacillus, pseudomonas, Alcaligenes and Nocardia carry out the seed enlarged culturing respectively, obtain the bacterium liquid of enlarged culturing, be that the bacterium liquid of the enlarged culturing of substratum 5-10% inserts respectively in the substratum of fermentor tank and carries out independent fermentation culture with weight, leavening temperature keeps 30-40 ℃, and fermentation time 48-72 hour, the air oxygen supply after fermenting process adopts mechanical stirring or feeds filtration.Obtain moving glue bacterium culture, comamonas culture, bacillus culture, pseudomonas culture, Alcaligenes culture and Nocardia culture.Bacteria content is in the culture of single Pseudomonas: hundred million/gram of 50-100.
Wherein, culture medium preparation: a. liquid nutrient medium: get peptone 10-20g, glucose 15-25g, glycerine 3-8g, sodium-chlor 3-8g, calcium chloride 1-3g, potassium primary phosphate 150-200mg, ferrous sulfate 25-35mg, manganous sulfate 20-25mg, zinc sulfate 5-10mg, water 1000mL, mixed dissolution; B. solid medium is prepared in following ratio: wheat bran 80-90%, Semen Maydis powder 10-20%; C. getting weight ratio and be 1: 1.1~1.2 solid medium and liquid nutrient medium mixes.
2, the culture that step 1 is obtained is pressed column weight amount umber and is carried out composite: 30 parts of moving glue bacterium cultures, 25 parts of comamonas cultures, 20 parts of bacillus cultures, 15 parts of pseudomonas cultures, 5 parts of Alcaligenes cultures, 5 parts of Nocardia cultures.Make the high-effective microorganism compound formulation.
3, the compound system of the high-effective microorganism that makes is enlarged domestication and cultivate, and will tame cultured microorganism bacterium liquid and add in Sewage treatment systems, specifically implementation step is as follows:
The 1st step: select a useful volume greater than 6 cubic metres container, the clear water (weighing 3.0 tons) that in container, adds 3.0 cubic metres, 3.0 cubic meter desire dispose of sewage (weighing 3.0 tons), mix the back and add 120 kg glucose, 30 kilograms of peptones, 24 kilograms of nitrogen-phosphorus-potassium compound fertilizers, 18 kilograms of trace element water-soluble fertilizers, regulate PH to 7-8, add 480 kilograms of composite and highly effective microorganism preparations, aeration or stirred 48 hours, make nutrient solution 1, nutrient solution 1 with volume 80%, promptly 4.8 cubic metres, extract out evenly to throw with pump and be spread across in each sewage biochemical treatment tank, stay 20% nutrient solution 1 promptly 1.2 cubic metres make Inoculant;
The 2nd step: in remaining 20% nutrient solution 1, i.e. 1.2 cubic metres of Inoculants, add 2.4 cubic metres clear water, 2.4 cubic metres of sewage that desire is handled, mix the back and add 120 kg glucose, 30 kilograms of peptones, 24 kilograms of nitrogen-phosphorus-potassium compound fertilizers, 18 kilograms of trace element water-soluble fertilizers, regulate PH to 7-8; Add 240 kilograms of composite and highly effective microorganism preparations, aeration or stir 48 hours after, nutrient solution 2, with the nutrient solution 2 of volume 80%, promptly 4.8 cubic metres, extract out evenly to throw with pump and be spread across in each sewage biochemical treatment tank, stay 20% nutrient solution 2 to make Inoculant;
The 3rd step: in remaining 20% nutrient solution 2, promptly make Inoculant for 1.2 cubic metres, all the other steps are with the 2nd step, make nutrient solution 3, with the nutrient solution 3 of volume 80%, promptly 4.8 cubic metres, extract even the throwing out with pump and be spread across in each sewage biochemical treatment tank, stay 20% nutrient solution 3 to make Inoculant;
The 4th step: in remaining 20% nutrient solution 3, promptly make Inoculant for 1.2 cubic metres, all the other steps are with the 2nd step, make nutrient solution 4, with the nutrient solution 4 of volume 80%, promptly 4.8 cubic metres, extract even the throwing out with pump and be spread across in each sewage biochemical treatment tank, stay 20% nutrient solution 3 to make Inoculant;
The 5th step: in remaining 20% nutrient solution 4, promptly make Inoculant for 1.2 cubic metres, all the other steps make nutrient solution 5 with the 2nd step, nutrient solution 5 all extracted out evenly to throw with pump be spread across in each sewage biochemical treatment tank;
Repeating the 1st~5 goes on foot 1 time.
Before not using technical scheme of the present invention, about UASB average effluent COD 5000mg/L, clearance 37.5%; Because contain organonitrogen in the sewage, the water outlet ammonia nitrogen raises to some extent, mean value is about 350mg/L; Because UASB goes out the water concentration height, SBR is difficult to bear the high loading influent concentration, so that SBR almost paralyses is inoperative, does not have removal effect.Implement after the present invention, about UASB average effluent COD 1500mg/L, clearance 81.25%; The SBR average effluent COD is less than 300mg/L, and clearance is more than 80%, and water outlet ammonia ammonia mean value is less than 15mg/L, and clearance is more than 95%.The qualified discharge requirement is satisfied in water outlet.
Embodiment 3: the enforcement the present invention of sewage work who to the biochemistry pool useful volume is 9000 cubic metres.
Sewage work's day is handled water yield 3600m 3/ d, treatment process are anaerobic filter+catalytic oxidation, and the biochemistry pool useful volume is 9000 cubic metres, and hydraulic detention time is 60 hours, and water inlet COD mean value 3500mg/L is about influent ammonia nitrogen mean value 240mg/L.Concrete steps are as follows:
1, the cultivation of bacterium: with driven glue bacterium, comamonas, genus bacillus, pseudomonas, Alcaligenes and Nocardia carry out the seed enlarged culturing respectively, obtain the bacterium liquid of enlarged culturing, the bacterium liquid of enlarged culturing is inserted in the fermentation tank culture medium by 5-10% carry out independent fermentation culture.Leavening temperature keeps 30-40 ℃, and fermentation time 48-72 hour, the air oxygen supply after fermenting process adopts mechanical stirring or feeds filtration.Obtain moving glue bacterium culture, comamonas culture, bacillus culture, pseudomonas culture, Alcaligenes culture and Nocardia culture.Bacteria content is in the culture of single Pseudomonas: hundred million/gram of 50-100.
Wherein, culture medium preparation: a. liquid nutrient medium is prepared than row by following: wheat bran 80-120g, white sugar 15-25g, sodium-chlor 3-8g, calcium chloride 1-3g, potassium primary phosphate 150-200mg, ferrous sulfate 25-35mg, manganous sulfate 20-25mg, zinc sulfate 5-10mg, water 1000mL; B. solid medium is prepared in following ratio: wheat bran 60-70%, cavings 30-40%, thin chaff 5-10%; C. get weight ratio and be 1: 1 solid medium and liquid nutrient medium.
2, the culture that step 1 is obtained is pressed column weight amount umber and is carried out composite: 28 parts of moving glue bacterium cultures, 22 parts of comamonas cultures, 18 parts of bacillus cultures, 17 parts of pseudomonas cultures, 7 parts of Alcaligenes cultures, 8 parts of Nocardia cultures.Make the high-effective microorganism compound formulation.
3, the compound system of the high-effective microorganism that makes is enlarged domestication and cultivate, and will tame cultured microorganism bacterium liquid and add in Sewage treatment systems, specifically implementation step is as follows:
The 1st step: select a useful volume greater than 30 cubic metres pond, the clear water (weighing 15 tons) that in the pond, adds 15 cubic metres, 15 cubic metres of pending sewage (weighing 15 tons), mix the back and add 450 kilograms of glucose, the 120 kg peptone, 90 kilograms of nitrogen-phosphorus-potassium compound fertilizers, 60 kilograms of strong Feng You trace element fertilizers (available from the safe legendary god of farming's scientific and technological development in Beijing three company limited), regulate PH to 7-8, add 2700 kilograms of high-effective microorganism microbial inoculums, aeration or stirred 40 hours, make nutrient solution 1, nutrient solution 1 with volume 90%, promptly 27 cubic metres, extract out evenly to throw with pump and be spread across in each sewage biochemical treatment tank, stay 10% nutrient solution 1 promptly 3 cubic metres make Inoculant;
The 2nd step: in remaining 10% nutrient solution 1, promptly make Inoculant for 3 cubic metres, add 13.5 cubic metres clear water, 13.5 cubic metres of pending sewage, mix the back and add 450 kilograms of glucose, 120 kg peptone, 90 kilograms of nitrogen-phosphorus-potassium compound fertilizers, 60 kilograms of strong Feng You trace element fertilizers (available from the safe legendary god of farming's scientific and technological development in Beijing three company limited), regulate PH to 7-8; Add 1350 kilograms of high-effective microorganism microbial inoculums, aeration or stir 40 hours after, nutrient solution 2, with the nutrient solution 2 of volume 90%, promptly 27 cubic metres, extract out evenly to throw with pump and be spread across in the sewage biochemical treatment tank, stay 10% nutrient solution 2 promptly 3 cubic metres make Inoculant;
The 3rd step: in remaining 10% nutrient solution 2, promptly make Inoculant for 3 cubic metres, all the other steps are with the 2nd step, make nutrient solution 3, with the nutrient solution 3 of volume 90%, promptly 27 cubic metres, extract even the throwing out with pump and be spread across in the sewage biochemical treatment tank, stay 10% nutrient solution 3 to be 3 cubic metres and make Inoculant;
The 4th step: in remaining 10% nutrient solution 3, promptly make Inoculant for 3 cubic metres, all the other steps are with the 2nd step, make nutrient solution 4, with the nutrient solution 4 of volume 90%, promptly 27 cubic metres, extract even the throwing out with pump and be spread across in the sewage biochemical treatment tank, stay 10% nutrient solution 4 to be 3 cubic metres and make Inoculant;
The 5th step: in remaining 10% nutrient solution 4, promptly make Inoculant for 3 cubic metres, all the other steps make nutrient solution 5 with the 2nd step, nutrient solution 5 usefulness pumps are extracted out all to throw be spread across in the sewage biochemical treatment tank.
Before not using technical scheme of the present invention, average effluent COD 1200mg/L, clearance 65.7%; Ammonia nitrogen almost there is not removal effect.Implement after the present invention, average effluent COD is less than 300mg/L, and clearance reaches 91.43%; Water outlet ammonia ammonia mean value is less than 15mg/L, and clearance is more than 93%.The qualified discharge requirement is satisfied in water outlet.
Have the following advantages in the foregoing description concrete operations:
(1) the microorganism reproductive speed is fast, cultivates domestication time weak point, can go out a large amount of dominant microfloras by fast breeding;
(2) cultured microorganism adaptability is strong, and difficult degradation, macromole, hazardous and noxious substances and ammonia nitrogen are had good adaptability and degradation effect;
(3) strong shock resistance when system is subjected to impacting (discharge of sewage increases suddenly or concentration of wastewater increases suddenly) or restarts, can start fast with recovery system and move;
(4) consumption of saving microorganism improves the microorganism utilising efficiency;
(5) can reduce input cost, have remarkable economic efficiency and social benefit;
(6) use is simple, easy to operate, and instant effect can be received good wastewater treatment efficiency in 5-10 days;
(7) strengthening synergy by high-effective microorganism and can improve the processing power and the treatment effect of sewage work, is the effective ways that new Sewage treatment systems starts fast and existing old sewage work is carried out dilatation and technological transformation.
The present invention both had been applicable to the processing of municipal sanitary sewage, various industrial sewages, also be applicable to the improvement that the rivers and lakes environment water pollutes, especially to the reluctant high density of traditional activated sludge process, macromole, be difficult to resolve fall, poisonous and harmful and high saliniferous organic sewage and high-ammonia-nitrogen sewage have unique treatment effect.
The above embodiment is the preferred embodiment that proves absolutely that the present invention lifts, and protection scope of the present invention is not limited thereto.Being equal to that those skilled in the art are done on basis of the present invention substitutes or conversion, all within protection scope of the present invention.Protection scope of the present invention is as the criterion with claims.

Claims (10)

1. a composite and highly effective microorganism preparation that is used for synergy of sewage treatment plant is characterized in that, the activeconstituents of described composite and highly effective microorganism preparation comprises: moving glue bacterium, comamonas, genus bacillus, pseudomonas, Alcaligenes and Nocardia.
2. composite and highly effective microorganism preparation according to claim 1 is characterized in that, described composite and highly effective microorganism preparation comprises the component of following parts by weight:
Moving glue bacterium culture 25-30 part,
Comamonas culture 20-25 part,
Bacillus culture 15-20 part,
Pseudomonas culture 15-20 part,
Alcaligenes culture 5-10 part,
Nocardia culture 5-10 part.
3. the method for preparing claim 1 or 2 described composite and highly effective microorganism preparations, it is characterized in that, comprise the steps: to move the glue bacterium, comamonas, genus bacillus, pseudomonas, Alcaligenes and Nocardia be single culture in substratum respectively, obtain moving glue bacterium culture, comamonas culture, bacillus culture, pseudomonas culture, Alcaligenes culture and Nocardia culture, mix, obtain composite and highly effective microorganism preparation according to described amount.
4. method according to claim 3 is characterized in that, comprises the steps:
1) will move the glue bacterium, comamonas, genus bacillus, pseudomonas, Alcaligenes and Nocardia be enlarged culturing respectively, obtains the bacterium liquid of enlarged culturing;
2) be that the bacterium liquid of the enlarged culturing of substratum 5-10% inserts respectively and carries out independent fermentation culture in the substratum with weight, obtain moving glue bacterium culture, comamonas culture, bacillus culture, pseudomonas culture, Alcaligenes culture and Nocardia culture.
5. method according to claim 4 is characterized in that step 2) described substratum is solid medium or liquid nutrient medium.
6. claim 1 or 2 described composite and highly effective microorganism preparations are used for the technology of synergy of sewage treatment plant, it is characterized in that, comprise the steps:
1) clear water and treatment sewage are mixed, add glucose, peptone, nitrogen-phosphorus-potassium compound fertilizer and trace element water-soluble fertilizer, regulate PH to 7-8, add described composite and highly effective microorganism preparation, aeration or stirring 24-48 hour, make nutrient solution 1, in the nutrient solution 1 input sewage biochemical treatment tank with volume 80~90%;
2) get the remaining nutrient solution 1 of step 1), add clear water and treatment sewage, add glucose, peptone, nitrogen-phosphorus-potassium compound fertilizer and trace element water-soluble fertilizer, regulate PH to 7~8, add described composite and highly effective microorganism preparation, aeration or stirring 24-48 hour make nutrient solution 2, in the nutrient solution 2 input sewage biochemical treatment tanks with 80~90% volumes;
3) get step 2) remaining nutrient solution 2, operation is with step 2), make nutrient solution 3, volume 80~90% nutrient solutions 3 are dropped in the sewage biochemical treatment tank;
4) get the remaining nutrient solution 3 of step 3), operation is with step 2), make nutrient solution 4, volume 80~90% nutrient solutions 4 are dropped in the sewage biochemical treatment tank;
5) get the remaining nutrient solution 4 of step 4), operation is with step 2), make nutrient solution 5, nutrient solution 5 is dropped in the sewage biochemical treatment tank.
7. technology according to claim 6 is characterized in that described trace element water-soluble fertilizer contains trace elements iron, copper, manganese, zinc, boron and molybdenum.
8. technology according to claim 6 is characterized in that, described method also comprises: repeating step 1)~and the operation of step 5) 1~5 time.
9. technology according to claim 6 is characterized in that,
The weight ratio of the add-on of clear water described in the step 1), treatment sewage, glucose, peptone, nitrogen-phosphorus-potassium compound fertilizer, trace element water-soluble fertilizer and composite and highly effective microorganism preparation is: 50: 50: 1~2: 0.3~0.5: 0.2~0.4: 0.1~0.3: 8~10;
Step 2)~step 5) in the remaining nutrient solution 1~4 any one, the weight ratio of clear water, treatment sewage, glucose, peptone, nitrogen-phosphorus-potassium compound fertilizer, trace element water-soluble fertilizer and composite and highly effective microorganism preparation add-on is: 10~20: 40~45: 40~45: 1~2: 0.3~0.5: 0.2~0.4: 0.1~0.3: 4~5.
10. technology according to claim 6 is characterized in that, in the described nutrient solution 1~5 any one dosage that drops into the sewage biochemical treatment tank be in the described sewage biochemical treatment tank sewage weight 0.1~1%.
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CN102583706A (en) * 2012-01-16 2012-07-18 宜态科环保技术(苏州)有限公司 Processing method for removing ammonia nitrogen through high-salt low-carbon industrial waste water continuous biological synergism
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CN112226390A (en) * 2020-10-28 2021-01-15 深圳市家家分类环保技术开发有限公司 Compound functional microbial inoculum for composting fermentation and kitchen waste composting method
CN115948288A (en) * 2022-12-06 2023-04-11 浙江大学 Aerobic efficient denitrification compound flora and application thereof
CN115948288B (en) * 2022-12-06 2023-09-26 浙江大学 Aerobic high-efficiency denitrification compound flora and application thereof

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