CN1384068A - Biological reinforcer for treating phenol-containing effluent - Google Patents
Biological reinforcer for treating phenol-containing effluent Download PDFInfo
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- CN1384068A CN1384068A CN 01113915 CN01113915A CN1384068A CN 1384068 A CN1384068 A CN 1384068A CN 01113915 CN01113915 CN 01113915 CN 01113915 A CN01113915 A CN 01113915A CN 1384068 A CN1384068 A CN 1384068A
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- bacterial strain
- phenol
- biological
- reinforcer
- strain
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Abstract
The present invention belongs to the field of sewage treatment and especially, relates to a biological engineering bacterial composition for treating effluent with high phenol content. The composition contains Norcardia as main component, pseudomonads and bacillus. The present invention aims at providing effective phenol-containing effluent treating measure.
Description
The invention belongs to a kind of field of sewage treatment, particularly relate to a kind of bioengineered strain that adopts and remove or the very high trade effluent compositions for use of degraded phenol content.
After the eighties in 20th century, All Around The World all is faced with the challenge of severe resource and environment, and water resources is very important special again natural resources, and he is the base substance that the mankind depend on for existence, is the restriction factor of human social.In recent years, because population growth and socio-economic development, the rate of growth of water requirement was very surprising, added the pollution of water waste and water resources, made the water resources worsening shortages that becomes.There is some evidence that the problem of water and shortage of water resources have become one of severe challenge of face of mankind nowadays.
Phenolic wastewater is one of principal pollutant in the trade effluents such as chemical industry, smelting, coking.Environmental pollution is particularly quite serious to water pollution, is one of main component of China's industrial pollution.There is quite strict standard in country to the discharging of phenolic wastewater, but since phenol to biological, particularly microorganism has broad spectrum toxicity, so the regulation effect of phenolic wastewater is not fully up to expectations always.
At problem set forth above, the invention provides a kind of bio-enhancer of administering phenolic wastewater, its purpose is to solve the problem that aspects such as conventional process phenol-containing wastewater effect is bad exist.
The present invention comes to be realized by the following technical programs:
A kind of bio-enhancer of administering phenolic wastewater is characterized in that: each composition in the said composition and mutual proportionlity (with by volume) as follows thereof:
Nocardia 5: Pseudomonas 3: genus bacillus 2,
Account for main advantage with Nocardia.
Advantage of the present invention and effect:
We develop a kind of to the extraordinary bio-enhancer of phenolic wastewater treatment effect at present, mainly be by biotechnology means screenings, tame out some phenol is had legs, have removal, degradation effect microorganism strains preferably, and according to a kind of biologically active agent that proportioning becomes that concerns between the bacterial strain.
Use the bio-enhancer Phenol-Containing Wastewater Treatment, can increase substantially treatment effect, make water quality reach the emission standard of country.
Below by embodiment the present invention is specifically described:
The screening of dominant strain and F-strain and domestication, be characteristics according to bacterial strain, we are formulated as different concns with pollutent, bacterium to be selected is pressed in the nutrient solution of pollutant load different concns from low to high, last device domestication is selected the bacterial strain that adaptive faculty is strong, removal efficient is high and is continued cultivation, domestication.And according to the characteristics of various microorganism belonging to genus and the cometabolism effect between the bacterial strain, make various microorganism belonging to genus in nutrient solution, occupy different ratios, continue to cultivate, tame, it cultivates the domestication condition: PH6.8～7.5, T20～30 ℃, 6～12 hours residence time, revolution 60～250 commentaries on classics/min, DO.3～6mg/L.
This method realizes as follows:
A, separation adaptability bacterial strain;
The nutrient solution of three concentration of preparation is in triangular flask from contaminated waste water, pollute in the nutrient solution that concentration of substrate is respectively 50,100,150mg/L, from contaminated water or mud, take small amount of sample in triangular flask, last shaking table is cultivated 24h, switching is incubated in the nutrient solution of another same concentration, repeatable operation one month, inducible strain produces the enzyme of a certain specific pollutant of degraded.
B, efficient, the special degradation property bacterial strain of screening;
Take the aseptic technique means, press the liquid diluting method,, select anti-pollution concentration height and divide pure the strong bacterial strain of contaminant removal capacity to pure bacterium with strains separation.
C, breeding strain;
Select the efficient bacterial strain of number strain (3～6 strain), adopt that selection by mutation, protoplastis merge, the plasmid transfering means, stimulate bacterial strain to change, bacterial strain continues to be respectively 50,100 in pollution concentration, shaking culture in the nutrient solution of 150mg/L, repeatedly for several times;
D, enlarged culturing are made finished product bacteria suspension or pulvis;
The top fermentation jar is cultivated, 30 ℃ of culture temperature, and incubation time 12～36 hours, pH value 6.8～7.4 makes strain growth to logarithmic phase, and bacterial strain quantity reaches 10
9Individual/as more than the ml, can be made into the suspension shape, but also low-temperature freeze drying to become pulvis;
E, be invested in the actual waste water system.
After breeding strain, enlarged culturing is made before finished product bacteria suspension or the pulvis, measures the degradability of efficient bacterial strain to pollutent, carries out best proportioning;
Measure the degradability of single bacterial strain to pollutent, hybrid bacterial strain is to the degradability of pollutent, select the bacterial strain that degradation capability is strong, growth is vigorous, strong to environment-adapting ability, the contaminant degradation clearance is reached more than 90%, carrying out from new proportioning, making it to bring into play the cometabolism effect between the bacterial strain.
By the above method that provides, can in containing the very high waste water of phenol, be mixed with following composition;
Nocardia 5: Pseudomonas 3: genus bacillus 2,
Account for main advantage with Nocardia.
Said composition can reach the ideal level to containing the very high wastewater treatment ability of phenol.
Claims (1)
1, a kind of bio-enhancer of administering phenolic wastewater is characterized in that: each composition in the said composition and mutual proportionlity (by volume) as follows thereof:
Nocardia 5: Pseudomonas 3: genus bacillus 2,
Wherein account for main advantage with Nocardia.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01113915 CN1384068A (en) | 2001-04-28 | 2001-04-28 | Biological reinforcer for treating phenol-containing effluent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01113915 CN1384068A (en) | 2001-04-28 | 2001-04-28 | Biological reinforcer for treating phenol-containing effluent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1384068A true CN1384068A (en) | 2002-12-11 |
Family
ID=4660612
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01113915 Pending CN1384068A (en) | 2001-04-28 | 2001-04-28 | Biological reinforcer for treating phenol-containing effluent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1384068A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100519736C (en) * | 2004-04-21 | 2009-07-29 | 中国科学院广州地球化学研究所 | Process for preparing immobilization compound microorganism preparation for controlling secondary pollution of river channel bed mud |
| CN101921709A (en) * | 2010-08-10 | 2010-12-22 | 北京三泰正方生物环境科技发展有限公司 | Composite high-efficiency microorganism preparation for synergy of sewage treatment plant and synergistic process |
-
2001
- 2001-04-28 CN CN 01113915 patent/CN1384068A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100519736C (en) * | 2004-04-21 | 2009-07-29 | 中国科学院广州地球化学研究所 | Process for preparing immobilization compound microorganism preparation for controlling secondary pollution of river channel bed mud |
| CN101921709A (en) * | 2010-08-10 | 2010-12-22 | 北京三泰正方生物环境科技发展有限公司 | Composite high-efficiency microorganism preparation for synergy of sewage treatment plant and synergistic process |
| CN101921709B (en) * | 2010-08-10 | 2013-05-01 | 北京三泰正方生物环境科技发展有限公司 | Composite high-efficiency microorganism preparation for synergy of sewage treatment plant and synergistic process |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |