CN102827786A - Lysinibacillus sp. for degrading S<2-> in petrochemical wastewater - Google Patents
Lysinibacillus sp. for degrading S<2-> in petrochemical wastewater Download PDFInfo
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- CN102827786A CN102827786A CN2012101145973A CN201210114597A CN102827786A CN 102827786 A CN102827786 A CN 102827786A CN 2012101145973 A CN2012101145973 A CN 2012101145973A CN 201210114597 A CN201210114597 A CN 201210114597A CN 102827786 A CN102827786 A CN 102827786A
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- lysinibacillus
- genus bacillus
- petrochemical wastewater
- methionin genus
- degrading
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Abstract
The invention relates to a Lysinibacillus sp. for degrading S<2-> in petrochemical wastewater. The Lysinibacillus sp. provided in the invention can efficiently degrade S<2-> in the petrochemical wastewater, and has a high application value in the removal of sulfide field components from the petrochemical wastewater. The Lysinibacillus sp. for degrading the S<2-> in the petrochemical wastewater is Lysinibacillus sp. Z39ay1, is preserved in China General Microbiological Culture Collection Center on December 20th, 2011, and has a preservation number of CGMCC No.5618. The Lysinibacillus sp. Z39ay1 can efficiently degrade the S<2-> in the petrochemical wastewater, and can be applied to the petrochemical wastewater processing field.
Description
Technical field
The present invention relates to the Methionin genus bacillus of negative sulfidion in the strain degraded petrochemical wastewater.
Background technology
Develop rapidly along with petroleum industry; Petrochemical wastewater is serious day by day to the pollution problem of environment; Odor pollution is one of major pollution in petrochemical wastewater; And in odorant pollutant, occupy the first place with the pollution of chalcogenide compound, therefore the research of the sulfur series foul smell gas by using material improvement aspect of petrochemical wastewater has been become the focus of attention of field of environment protection.At present, the improvement method for sulfide in the petrochemical wastewater mainly comprises physics method, chemical method and biological process.Biological process is to utilize the Decomposition of mikrobe to repugnant substance, makes it to be oxidized to final product, thereby reaches odorlessization, innoxious a kind of method.Characteristics such as this method is because of having the processing efficiency height, and non-secondary pollution, required equipment simply are convenient to operation, and cost and management maintenance are convenient and become the developing direction of odor treatment have become the main stream approach of a lot of national odor pollutions control application at present.
The research of deodorization mikrobe is the key problem of biological deodorization; Though at present both at home and abroad the scientific research personnel has been separated to the bacterium of the multiple chalcogenide compound that can be used for degrading; But the bacterial species for negative sulfidion has Degradation is still less; Because therefore the acting in conjunction of the microorganism species that biological deodorization needs is constantly screened the high-efficiency desulfurization bacterium that makes new advances and is remained the key issue in the odor treatment research.
Summary of the invention
The present invention provides a strain Methionin genus bacillus (Lysinibacillus sp.), but negative sulfidion in the efficient degradation petrochemical wastewater has higher using value to removing of sulfide odor pollutant in the petrochemical wastewater.
The present invention degrades, and the Methionin genus bacillus of negative sulfidion is Methionin genus bacillus (Lysinibacillus sp.) Z in the petrochemical wastewater
39ay
1, being deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC), the preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and preservation date is on December 20th, 2011, and deposit number is CGMCC No.5618; Methionin genus bacillus Z of the present invention
39ay
1Be gram-positive microorganism, shaft-like (like Fig. 1); After cultivating 2 days on the nutrient broth solid medium that with sodium sulphite is the sulphur source, lawn is circular, and oyster white is translucent, surface wettability, intermediate projections (like Fig. 2), cultivate a week after, lawn becomes pale pink, and is opaque.
Methionin genus bacillus Z of the present invention
39ay
1Oxydase reaction is negative, and the starch hydrolysis experiment is the result be positive.Methionin genus bacillus Z of the present invention
39ay
1Carry out growth metabolism with sodium sulphite for the sulphur source, can utilize Sulfothiorine to be the sulphur source.
Methionin genus bacillus of the present invention (Lysinibacillus sp.) Z
39ay
1Analyze through 16S rDNA sequence alignment; The homology of 16S rDNA sequence that belongs to (Lysinibacillus) with Lysinibacillus is the highest; Similarity is 96%～100%, confirms Methionin genus bacillus Z through combining morphological features, growth conditions, Physiology and biochemistry qualification result
39ay
1Belong to Lysinibacillus and belong to (Lysinibacillus).
Methionin genus bacillus Z of the present invention
39ay
1Can on the nutrient broth medium that with sodium sulphite is the sulphur source, grow.The nutrient broth medium (1L) that with sodium sulphite is the sulphur source is by 10.0g peptone, 2.0g Carnis Bovis seu Bubali cream, 5.0gNaCl, 1.6g Na
2S.9H
2The zero(ppm) water of O and surplus is formed, and adjust pH is 7.0～7.2.Methionin genus bacillus Z
39ay
1Optimum growth temperature be 28～30 ℃, the pH value of right growing environment is 7.0～7.2.
Methionin genus bacillus Z of the present invention
39ay
1Can bear sulfidion in the efficient degradation petrochemical wastewater, with Methionin genus bacillus Z
39ay
1Access contains Na
2S9H
2In the nutrient broth medium of O, after shaking table was cultivated a week under 30 ℃, 160rpm condition, the rate of separating of falling negative sulfidion can reach 42.65%; Methionin genus bacillus Z of the present invention
39ay
1Efficiently bear sulfidion in the petrochemical wastewater.
Methionin genus bacillus of the present invention (Lysinibacillus sp.) Z
39ay
1Belong to Lysinibacillus and belong to (Lysinibacillus); Be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC); The preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Preservation date is on December 20th, 2011, and deposit number is CGMCC No.5618.
Description of drawings
Fig. 1 is Methionin genus bacillus Z of the present invention
39ay
1Microphotograph; Fig. 2 is Methionin genus bacillus Z of the present invention
39ay
1The colonial morphology photo; Fig. 3 is Methionin genus bacillus Z
39ay
116S rDNA sequence homology comparison figure with close bacterial strain.
Embodiment
Technical scheme of the present invention is not limited to following cited embodiment, also comprises the arbitrary combination between each embodiment.
Embodiment one: the Methionin bacillus mutant of negative sulfidion is Methionin genus bacillus (Lysinibacillus sp.) Z in this embodiment degraded petrochemical wastewater
39ay
1, being deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC), the preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and preservation date is on December 20th, 2011, and deposit number is CGMCC No.5618.
This embodiment Methionin genus bacillus Z
39ay
1Be gram-positive microorganism, shaft-like; After cultivating 2 days on the nutrient broth solid medium that with sodium sulphite is the sulphur source, lawn is circular, and oyster white is translucent, surface wettability, intermediate projections, cultivate a week after, lawn becomes pale pink, and is opaque.
With reference to " the outstanding Bacteria Identification handbook of uncle the 8th edition and " common bacteria system identification handbook " are to Methionin genus bacillus (Lysinibacillus sp.) Z
39ay
1Carrying out Physiology and biochemistry identifies: Methionin genus bacillus Z
39ay
1Oxydase reaction is negative, and the starch hydrolysis experiment is the result be positive.Methionin genus bacillus Z
39ay
1Carry out growth metabolism with sodium sulphite for the sulphur source, can utilize Sulfothiorine to be the sulphur source.
This embodiment Methionin genus bacillus Z
39ay
1Can on the nutrient broth medium that with sodium sulphite is the sulphur source, grow.The nutrient broth medium (1L) that with sodium sulphite is the sulphur source is by 10.0g peptone, 2.0g Carnis Bovis seu Bubali cream, 5.0gNaCl, 1.6gNa
2S.9H
2The zero(ppm) water of O and surplus is formed, and adjust pH is 7.0～7.2.The nutrient broth solid medium that with sodium sulphite is the sulphur source be above-mentioned be to add 8.5～9.0g agar powder in the nutrient broth medium in sulphur source with sodium sulphite.
This embodiment Methionin genus bacillus Z
39ay
1Optimum growth temperature be 28～30 ℃, the pH value of right growing environment is 7.0～7.2.
Embodiment two: this embodiment Methionin genus bacillus (Lysinibacillus sp.) Z
39ay
1Screen in the active sludge by the aeration tank of petroleum chemical plant and obtain.Screening method carries out according to the following steps: draw active sludge mud 10mL, insert enrichment culture in the 200mL primary dcreening operation substratum, 30 ℃, the 160r/min shaking culture was taken out after 3 days, left standstill 30 minutes.From supernatant, take out 40mL then, pour the fresh primary dcreening operation substratum of 40mL into, add 1.2mL Na
2S9H
2O solution (0.24g/L) repeats above-mentioned steps later on every three days, makes Na in the nutrient solution
2S9H
2The content of O increases to 4.8g at last, continues then to cultivate three days, leaves standstill 30min, draws supernatant 25mL, changes the fresh primary dcreening operation substratum of 25mL over to and (contains 4.8gNa
2S9H
2O) 30 ℃ of 160r/min shaking culture 24h therefrom take out 20mL and are added in the fresh primary dcreening operation substratum of 200mL, and 30 ℃ of 160r/min shaking culture 24h get mixed bacteria liquid; Draw mixed bacteria liquid 1.0mL then, inject the SPSS that 99.0mL is equipped with granulated glass sphere, the 200r/min 30min that vibrate, and by the serial dilution of 10 times of gradient dilution methods to proper concn, absorption 10
-6, 10
-7, 10
-8, 10
-9With 10
-10Each 0.1mL of diluent evenly coats the sulfide sensitive organism respectively and separates on the selection plate culture medium, builds the ware lid, is inverted in 30 ℃ of thermostat containers, cultivates 24～48h, and each extent of dilution is done 2～3 and repeated dull and stereotyped.Observe the colony growth situation on the flat board, the different shape independence bacterium colony that the picking growth is quick and vigorous, correspondence places 30 ℃ of thermostat containers containing the purifying of ruling on the primary dcreening operation slant medium of 4.8g/L sodium sulphite, cultivates 24～48h, gets the bacterial strain of preliminary screening.Then with the inoculation of preliminary screening to containing 1.6g/LNa
2S9H
2Fall the sulphur experiment in the LB liquid nutrient medium of O, therefrom screening obtains desulfurization bacterial strain efficiently.Utilize the square position strok method to repeat purifying, separation at last, promptly obtain Methionin genus bacillus Z
39ay
1
Said primary dcreening operation substratum (1000mL) is by 2.0g KPH
2PO
4, 3.0g Carnis Bovis seu Bubali cream, 15.0g peptone, 3.0gNaCl, 2.0g Na
2HPO
4.12H
2O, 3.0g yeast extract paste, 10.0g NaS
2O
3, 1.2g～4.8g Na
2S.9H
2The zero(ppm) water of O and surplus is formed, and pH is 7.0, in 0.1MPa (121 ℃) autoclaving 25min.The primary dcreening operation slant medium is in above-mentioned primary dcreening operation substratum, to add the 8.5g agar powder.
The sulfide sensitive organism separates selects plate culture medium by 2.0g KPH
2PO
4, 3.0g Carnis Bovis seu Bubali cream, 15.0g peptone, 3.0g NaCl, 2.0g Na
2HPO
4.12H
2O, 3.0g yeast extract paste, 10.0g NaS
2O
3, 1.2g～4.8g Na
2S.9H
2The zero(ppm) water of O, 8.5g agar powder and surplus is formed.
The Methionin genus bacillus Z that screening is obtained
39ay
1Carry out Molecular Identification, with Methionin genus bacillus Z
39ay
1Send it to Shanghai living worker's biotechnology ltd and check order Methionin genus bacillus Z
39ay
116S rDNA sequence length be 1441bp; Its sequence is shown in SEQ ID NO:1; 16S rDNA sequence among sequencing result and the GenBank is carried out the homology compare of analysis; As shown in Figure 3 with the 16S rDNA gene order homology comparison figure of close bacterial strain; The homology of 16S rDNA sequence that belongs to (Lysinibacillus) with Lysinibacillus is the highest, and similarity is 96%～100%, confirms Methionin genus bacillus Z through combining morphological features, growth conditions, Physiology and biochemistry qualification result
39ay
1Belong to Lysinibacillus and belong to (Lysinibacillus).
Utilize static test to investigate Methionin genus bacillus Z39ay
1To the degradation capability of sodium sulphite, concrete grammar is: contain Na being equipped with
2S9H
2(every liter by 10.0g peptone, 2.0g Carnis Bovis seu Bubali cream, 5.0gNaCl, 1.6g Na for the 100mL nutrient broth medium of O
2S.9H
2The zero(ppm) water of O and surplus is formed, and the pH value is 7.0～7.2) in, (bacterial concentration is OD to insert 5mL bacterium liquid
600=1.3683), at 30 ℃, after shaking table is cultivated a week under the 160rpm condition, adopt the content of the negative sulfidion of iodometric determination, the rate of separating of falling negative sulfidion can reach 42.65%; Show Methionin genus bacillus Z39ay
1The sulfur-bearing simulated wastewater had the efficient degradation effect.
Claims (1)
1. the Methionin genus bacillus of negative sulfidion in the strain degraded petrochemical wastewater, the Methionin genus bacillus of negative sulfidion is Methionin genus bacillus (Lysinibacillus sp.) Z in the petrochemical wastewater that it is characterized in that degrading
39ay
1, being deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, the preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and preservation date is on December 20th, 2011, and deposit number is CGMCC No.5618.
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|---|---|---|---|
| CN2012101145973A CN102827786A (en) | 2012-04-18 | 2012-04-18 | Lysinibacillus sp. for degrading S<2-> in petrochemical wastewater |
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|---|---|---|---|
| CN2012101145973A CN102827786A (en) | 2012-04-18 | 2012-04-18 | Lysinibacillus sp. for degrading S<2-> in petrochemical wastewater |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103421702A (en) * | 2012-06-28 | 2013-12-04 | 华中农业大学 | Bacteria Lysinibacillus sp. for adsorbing gold and antimony |
| CN107345213A (en) * | 2017-06-07 | 2017-11-14 | 中国科学院上海高等研究院 | A kind of lysine bacillus of cow manure acid sordes of degrading and its application |
| CN110777085A (en) * | 2018-07-31 | 2020-02-11 | 上海交通大学 | Cadmium-resistant nightshade growth-promoting rhizosphere lysine bacillus and application thereof |
| CN111100824A (en) * | 2020-01-21 | 2020-05-05 | 暨南大学 | Bacillus and application thereof in denitrification and desulfurization in aquaculture water |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101497869A (en) * | 2009-01-22 | 2009-08-05 | 河北科技大学 | Predominating Population for removing ethanethiol and introduction domestication method thereof |
-
2012
- 2012-04-18 CN CN2012101145973A patent/CN102827786A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101497869A (en) * | 2009-01-22 | 2009-08-05 | 河北科技大学 | Predominating Population for removing ethanethiol and introduction domestication method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 周瑞平等: "偏高温大曲中一株嗜热细菌的分离与鉴定", 《酿酒科技》, no. 187, 31 December 2010 (2010-12-31), pages 36 - 38 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103421702A (en) * | 2012-06-28 | 2013-12-04 | 华中农业大学 | Bacteria Lysinibacillus sp. for adsorbing gold and antimony |
| CN103421702B (en) * | 2012-06-28 | 2015-03-11 | 华中农业大学 | Bacteria Lysinibacillus sp. for adsorbing gold and antimony |
| CN107345213A (en) * | 2017-06-07 | 2017-11-14 | 中国科学院上海高等研究院 | A kind of lysine bacillus of cow manure acid sordes of degrading and its application |
| CN107345213B (en) * | 2017-06-07 | 2020-03-17 | 中国科学院上海高等研究院 | Lysine bacillus for degrading dairy cow excrement acid odor and application thereof |
| CN110777085A (en) * | 2018-07-31 | 2020-02-11 | 上海交通大学 | Cadmium-resistant nightshade growth-promoting rhizosphere lysine bacillus and application thereof |
| CN110777085B (en) * | 2018-07-31 | 2022-11-25 | 上海交通大学 | Cadmium-resistant nightshade growth-promoting rhizosphere lysine bacillus and application thereof |
| CN111100824A (en) * | 2020-01-21 | 2020-05-05 | 暨南大学 | Bacillus and application thereof in denitrification and desulfurization in aquaculture water |
| CN111100824B (en) * | 2020-01-21 | 2021-11-05 | 暨南大学 | Bacillus and application thereof in denitrification and desulfurization in aquaculture water |
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Application publication date: 20121219 |