CN111518715B - Sulfonamide antibiotic synergistic degradation bacteria and application thereof - Google Patents
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Abstract
The invention provides a sulfonamide antibiotic cooperative degradation bacterium and application thereof, wherein the sulfonamide antibiotic cooperative degradation bacterium comprises arthrobacter pseudobacter P27, is preserved in the China general microbiological culture Collection center, and has the preservation date: 12 months 03 days 2019; the preservation number is: CGMCC No. 19070; nocardioides N27, deposited in China general microbiological culture Collection center, with the preservation date: 12 months 03 days 2019; the preservation number is: CGMCC No. 19071. According to the technical scheme, the two strains of bacteria are compounded to be capable of synergistically completing the complete degradation of sulfamethoxazole and nitrogen heterocyclic product 3-amino-5-methylisoxazole, and in addition, the strain P27 can also degrade one side of benzene rings of various sulfonamides antibiotics, so that the degradation substrate spectrum is wide, and a new idea and a new strain resource are provided for the deep removal of sulfonamides antibiotics.
Description
Technical Field
The invention relates to the field of microorganisms and biodegradation application thereof, in particular to sulfonamide antibiotic synergistically degrading bacteria and application thereof.
Background
The sulfanilamide antibiotics are novel pollutants closely related to daily life of people, the antibacterial activity of the sulfanilamide antibiotics is mainly dependent on the similarity of the structure of the sulfanilamide antibiotics and p-aminobenzoic acid, the sulfanilamide antibiotics and the p-aminobenzoic acid compete for dihydrofolate synthetase, synthesis of bacterial dihydrofolate is inhibited, synthesis of bacterial nucleic acid is influenced, and further growth and reproduction of bacteria are prevented. Although the sulfonamide antibiotics mainly exist in trace form in water body environment, the sulfonamide antibiotics can be continuously input into the environment through various ways, and compared with other medicines, the sulfonamide antibiotics have the characteristics of slow degradation rate, strong hydrophilicity, low volatility and the like, so that the sulfonamide antibiotics are in a 'lasting' existing state; importantly, the usage amount of the sulfonamide antibiotics in the livestock and poultry breeding industry is huge. The continual accumulation of residual antibiotics in the environment can create serious risks to the ecosystem and human health. The scholars think that the long-term low-dose antibiotic residue forces the microorganisms to evolve to generate antibiotic resistance genes, and the evolution of multi-drug resistant bacteria brings great risks to human health and ecological environment. Therefore, the effective removal of antibiotics from the source (sewage treatment plant) and the prevention or the minimization of the environmental emission become the research hotspots of researchers in recent years, and are also the difficulties in the environmental biotechnology field.
The removal studies on sulfonamides antibiotics have mainly focused on physicochemical methods (photocatalysis, adsorption, advanced oxidation) and biodegradation. The physical method has the defects of low universality, difficult catalyst recovery, high cost, possible secondary pollution and the like. In contrast, the removal of sulfonamide antibiotics by microbial degradation is an economic and effective green sustainable approach, and has important significance in reducing environmental pollution caused by the antibiotics and reducing ecological risks caused by the antibiotics.
At present, more reports are reported about screening of degrading bacteria of sulfonamide antibiotics,Microbacterium，Rhodococcus，Achromobacter，Ralstonia，Brevundimonas，Pseudomonas，Acinetobacter，Variovorax，AlcaligenesandOchrobactrumthe bacterial genera have reported that the bacterial genus has the degradation capability of sulfonamide antibiotics, but the problem of insufficient degradation depth generally exists, the degradation process is accompanied with the stable accumulation of nitrogen heterocyclic products, and the discharge of nitrogen-containing organic matters inevitably causes certain damage to the environment. Therefore, screening for high potency sulfa antibioticsBacteria are dissolved, the sulfonamide antibiotics are completely removed, and the microbial technical support is provided for bioremediation of the polluted environment.
Disclosure of Invention
Aiming at the technical problems, the invention discloses a synergistic degradation bacterium for sulfonamide antibiotics and application thereof, wherein the combination of two strains of bacteria can synergistically complete the complete degradation of SMX (sulfamethoxazole) and nitrogen heterocyclic product 3-amino-5-methylisoxazole (3A 5 MI), so that sulfamethoxazole is taken as a target pollutant, a new thought and a new strain resource are provided for the deep removal of the sulfonamide antibiotics, the defects of low degradation depth and stable accumulation of nitrogen heterocyclic product in the degradation process of the sulfonamide antibiotics treated by the current biological method are overcome, high-efficiency degradation strain resources are provided for the bioremediation of widely used sulfonamide antibiotics such as SMX, and the like, and the synergistic degradation bacterium has very important theoretical and application values.
In contrast, the technical scheme adopted by the invention is as follows:
sulfonamide antibiotic co-degrading bacteria comprising:
arthrobacter paradonii (Paenarthrobacter sp.) P27, deposited in the China general microbiological culture Collection center, with the preservation date: 12 months 03 days 2019; the preservation number is: CGMCC number 19070;
nocardioid bacterium (A), (B), (C), (Nocardioidessp.) N27, deposited in the China general microbiological culture Collection center, with the preservation date: 12 months 03 days 2019; the preservation number is: CGMCC number 19071;
the preservation address is the microbiological research institute of China academy of sciences, No. 3, Xilu No.1, Beijing, Chaoyang, Beijing.
Wherein P27 is a strain degrading on the benzene ring side of SMX and is identified as Arthrobacter paradonoides (A)Paenarthrobactersp.); n27 is a SMX azacyclo side product 3A5MI degrading bacterium, and is identified as Nocardia-like bacteriaNocardioides sp.）。
The main biological characteristics of P27 and N27 are: the bacterial colonies are all gram-positive, the bacterial colonies are all in short rod shapes, on an R2A culture medium, the bacterial colonies are milky white and have irregular sawtooth-shaped edges, and the bacterial colonies are faint yellow and have regular and smooth round edges.
The P27 and N27 are used as SMX synergistic degradation bacteria, play a role in synergistic degradation and are mainly embodied as follows: under aerobic conditions, in an inorganic salt culture medium with SMX as a sole carbon source, P27 can degrade one side of a benzene ring of SMX, and simultaneously generate a degradation product 3A5MI, so that carbon sources and energy sources are provided for growth and propagation of the strain N27, and thus synergistic and complete degradation of SMX is achieved.
Furthermore, under aerobic conditions, the P27 takes SMX as a unique carbon source and energy source, and is used for self growth and propagation by assimilating one side of a benzene ring of the SMX, and meanwhile, a nitrogen heterocyclic ring product 3A5MI is stably accumulated, and the N27 takes an SMX degradation product 3A5MI as a unique carbon source and energy source for self growth and propagation.
The invention realizes the efficient and complete removal of the sulfonamide antibiotics through two strains of activated sludge bacteria, overcomes the defects of low degradation depth and stable accumulation of nitrogen heterocyclic products in the degradation process of the sulfonamide antibiotics treated by the current biological method, provides efficient degradation strain resources for bioremediation of widely used sulfonamide antibiotics such as SMX and the like, and has very important theoretical and application values.
The 16S rRNA gene sequences of P27 and N27 provided by the invention are respectively shown as SEQ ID NO.1 and SEQ ID NO. 2.
The P27 not only can act on the benzene ring side of SMX, but also has the degradation capability on various sulfonamides antibiotics, can complete the degradation of the benzene ring side within 30-54 h at the initial concentration of 50 mg/L, and simultaneously stably accumulates corresponding nitrogen heterocyclic products. Wherein the sulfa antibiotic comprises at least one of Sulfamethoxazole (SMX), sulfapyridine, sulfamethazine, sulfamethizole, sulfachloropyridazine, sulfadimethoxine, sulfamethazine, sulfanilamide-5-methoxypyrimidine, sulfadiazine and sulfamonomethoxine.
Further, the arthrobacter pseudotuberculosis P27 and the nocardiopsis pseudotuberculosis N27 are separated from activated sludge of a sewage treatment plant.
The invention discloses application of the sulfonamide antibiotic synergistic degradation bacteria, and the sulfonamide antibiotic synergistic degradation bacteria are used for synergistic degradation of sulfonamide antibiotics. Furthermore, the synergistic degradation bacteria of the sulfonamide antibiotics are applied to degradation of residual sulfonamide antibiotics in sewage, wastewater, substrate and soil.
Further, the degradation environment for synergistic degradation of bacteria by the sulfonamide antibiotics is as follows: in an inorganic salt environment, the temperature is 15-40 ℃, the initial pH value is 5-9, and under an aerobic condition.
Further preferably, the temperature is 28-32 ℃, the initial pH value is 6.8-7.0, and the pH value is aerobic.
Further, the Arthrobacter paradonii P27 and the Nocardia-like N27 are added into an inorganic salt culture medium which takes SMX as a unique carbon source and energy source to carry out synergistic degradation of the SMX, wherein the initial concentration of the SMX is 1.04-53.02 mg/L.
The two strains of the SMX synergistic degradation bacteria P27 and N27 provided by the invention are separately cultured and supplied with carbon sources in an aerobic inorganic salt culture medium with the optimal temperature of 30 ℃ and the optimal initial pH of 6.8-7.0, and the strain P27 can completely degrade 50 mg/L of SMX within 20 h and stably generate an N-heterocyclic product 3A5MI with equal molar equivalent. The strain N27 can completely degrade 50 mg/L of 3A5MI within 40 h, and no product is accumulated.
If the inorganic salt medium is added into an inorganic salt medium taking SMX as a unique carbon source and energy source, and the synergistic degradation condition of the inorganic salt medium on the SMX is examined, the accumulation phenomenon of 3A5MI originally accompanying the SMX degradation disappears under the three initial concentrations of 1.04 mg/L, 5.88 mg/L and 53.02 mg/L, particularly under the medium-low concentration, 3A5MI is not detected in the degradation process, and the removal rates of the SMX can reach 87%, 100% and 100% respectively at 8 h, 14 h and 19 h. The result shows that the two strains of bacteria can efficiently remove the low-concentration and high-concentration sulfonamide antibiotics in a synergistic interaction mode, provide a new degraded strain resource for the bioremediation of the sulfonamide antibiotics, and have good application prospects.
The formula of the inorganic salt culture medium is preferably as follows: NaCl 1 g, NH4NO3 1 g， K2HPO4•3H2O1.5 g，KH2PO4 0.5 g，MgSO4•7H2O0.2 g, make up to 1000 mL of distilled water, and adjust pHTo 6.8-7.0.
Compared with the prior art, the invention has the beneficial effects that:
first, the invention discloses Arthrobacter paradoniiPaenarthrobactersp, P27) can degrade 10 kinds of sulfanilamide antibiotics, has broad-spectrum sulfanilamide antibiotic detoxification and degradation capability, has degradation efficiency remarkably higher than that of reported strains, and has huge bioremediation potential. No arthrobacterium paradoxum (B) is found in the prior artPaenarthrobactersp.) can degrade a plurality of sulfonamide antibiotics simultaneously, and the systematic classification status, the degradation substrate spectrum and the degradation efficiency of the degrading bacteria disclosed by the invention are different.
Secondly, the Nocardia-like bacteria (A), (B) and (C) disclosed in the inventionNocardioidessp, N27) can completely degrade 50 mg/L of 3A5MI within 40 h, has high degradation efficiency, and provides important degrading bacteria resources for ensuring effective reduction of total nitrogen in the nitrogenous sulfonamide antibiotics. No nocardioid bacteria (A), (B) are availableNocardioidessp.) a nitrogen heterocyclic product capable of degrading the sulfonamide antibiotics, 3-amino-5-methylisoxazole (3A 5 MI).
Third, the present invention discloses a strain (Paenarthrobactersp, P27) and (Nocardioidessp, N27) can obtain 100 percent of SMX removal rate within 20 hours under neutral aerobic condition, and complete efficient synergistic degradation of different SMX concentrations. The two degrading bacteria are separated from the activated sludge of a sewage treatment plant, can be well adapted to engineering application under natural conditions, has greatly reduced cost compared with a physical and chemical method, is green and free from toxic by-products, and has important significance for biologically strengthening and repairing sulfonamide antibiotics in the environments of sewage, waste water, soil and the like. At present, no degradation mode of activated sludge bacteria through synergistic interaction is available to realize complete degradation of sulfonamide antibiotics, including efficient complete degradation of nitrogen heterocyclic ring products.
Drawings
FIG. 1 is a photograph showing colonies of the sulfonamide antibiotic synergistically degrading bacteria of the present invention; wherein, the strain isPaenarthrobactersp. P27 at R2Photograph of colony on plate A, strainNocardioidessp, N27 at R2A flat platePhotograph of the colony above.
FIG. 2 is a transmission electron micrograph of the sulfonamide antibiotic synergistically degrading bacteria of the present invention, wherein (r) is a bacterial strainPaenarthrobacterTransmission electron micrograph of sp, P27; ② is a strainNocardioidesTransmission electron micrograph of sp. N27.
FIG. 3 shows a strain constructed based on the 16S rRNA gene of the present inventionPaenarthrobacterA phylogenetic tree of sp. P27.
FIG. 4 shows a strain constructed based on the 16S rRNA gene of the present inventionNocardioidesA phylogenetic tree of sp. N27.
FIG. 5 is a graph showing the degradation effect of the strain P27 on sulfamethoxazole under the condition of single culture.
FIG. 6 is a graph showing the degradation effect of the strain N27 on 3-amino-5-methylisoxazole under the condition of single culture in the invention.
FIG. 7 is a substrate degradation spectrum of nine sulfanilamide antibiotics by the strain P27 of the present invention.
FIG. 8 is a graph showing the synergistic effect of the strains P27 and N27 of the present invention on the degradation of sulfamethoxazole at different concentrations, wherein (1) the concentration is 1.04 mg/L, (2) the concentration is 5.88 mg/L, and (3) the concentration is 53.02 mg/L.
Detailed Description
The invention is described below with reference to the accompanying drawings and specific embodiments, and modifications made to the parts and parameters of the invention without departing from the spirit and substance of the invention are deemed to be within the scope of the invention.
The chemical reagents used in the practice of this example were all commercially available analytical grade and above, and standard products such as Sulfamethoxazole (SMX), 3-amino-5-methylisoxazole (3A 5 MI) were purchased from Allantin reagent company (Shanghai), Sigma (Sigma-Aldrich, USA), and the like. Sulfonamide antibiotics and 3-amino-5-methyl isoxazole were prepared into high concentration mother liquor (20 g/L) using 0.1M NaOH solution and ultrapure water, respectively.
The inorganic salt culture medium comprises the components as described in the invention, and is sterilized at high temperature and high pressure.
Example 1
Separation and identification of SMX benzene ring side degrading bacterium P27 and product degrading bacterium N27
1.1 isolation and purification of Strain P27
The bacteria source is residual sludge collected from a certain sewage treatment plant of Harbin, 50 mg/L of SMX is added into a 250 mL conical flask (containing 100 mL of sterilized inorganic salt culture medium) by mother liquor, and respectively inoculated with approximately 5 g of a muddy water mixed sample, and the mixture is cultured in a constant temperature shaking table at 30 ℃ and the rotating speed of 150 rpm. After 7-8 days of culture, the medium was prepared in the same manner and 10% volume enriched solution was transferred to fresh medium, respectively. And continuously switching for many times until no obvious sludge particles exist in the enrichment solution and a uniform milky turbid liquid is presented, and then measuring the SMX degradation effect of the enrichment solution. Strains were isolated and purified by dilution plating and streaking using plates containing 50 mg/L of SMX-inorganic salt (SMX-MM). The results show that two morphologically distinct colonies were always observed in the dilution-coated SMX-MM plates at different dilution ratios. When representative colonies among the two colonies were selected and streaked, the growth of the colonies was observed only on a plate of a certain bacterial origin, and the colony was designated as P27. The streaking operation was repeated several times, and the purified strain P27 was confirmed for its sulfamethoxazole degrading ability in an inorganic salt medium and stored in a refrigerator at-80 ℃ with glycerol.
1.2 isolation and purification of Strain N27
By verifying the sulfamethoxazole degrading capability of the strain P27, the strain P27 is found to not completely degrade SMX, and with the degradation of the SMX, an equivalent molar equivalent of a nitrogen heterocyclic product 3A5MI is accumulated, and the product is not further degraded even if the culture time is prolonged. Compared with the strain P27, the enrichment solution has stable and efficient complete degradation capability of SMX, and no intermediate product 3A5MI is left. Therefore, the existence of the degradation bacteria of the intermediate product 3A5MI in the enrichment liquid can be judged. The carbon source was switched from SMX to 3A5MI, and the previous dilution coating and streaking methods were repeated to isolate and purify the strain, which was designated as strain N27. The streaking operation was repeated several times, and the purified strain N27 was determined for its 3A5MI degradability in mineral salt medium and stored in a refrigerator at-80 ℃ with glycerol.
1.3 phylogenetic status identification of strains P27 and N27
Two strains of bacteria participating in the degradation process of the sulfonamide antibiotics are separated from the enrichment solution, and the separated and purified strains are subjected to simple physiological and biochemical analysis and 16S rRNA gene sequencing identification. The colony morphology and the observation result of a transmission electron microscope are shown in the attached figures 1 and 2, and the main biological characteristics of the strain P27 are as follows: gram staining reaction positive, the thallus is short rod-shaped, at R2The colony on the plate A is milky white and has irregular sawtooth-shaped edges. The sequence is highly similar toPaenarthrobacterThe 16S rRNA gene sequence of the genus is shown in a sequence table NO.1, and the phylogenetic tree is shown in an attached figure 3. The bacterium is identified as the bacterium by combining the physiological and biochemical characteristics of the bacteriumPaenarthrobactersp. named asPaenarthrobactersp. P27. The main biological characteristics of the strain N27 are as follows: gram staining reaction positive, the thallus is short rod-shaped, at R2A yellowish, smooth-edged round colony was formed on the A plate. The sequence is highly similar toNocardioidesThe 16S rRNA gene sequence of the genus is shown in a sequence table NO.2, and the phylogenetic tree is shown in an attached figure 4. The bacterium is identified as the bacterium by combining the physiological and biochemical characteristics of the bacteriumNocardioidessp. named asNocardioidessp. N27. Both strains have been handed over to China general microbiological culture Collection center (West Lu No.1, Ministry of China academy of sciences, No. 3, Beijing, Chaoyang, North Cheng, the collection date is 2019, 12 months and 03 days, and the collection numbers are CGMCC number 19070 and CGMCC number 19071 respectively.
Example 2
Degradation efficiency analysis of separately cultured SMX benzene ring side degrading bacteria P27 and nitrogen heterocyclic ring product degrading bacteria N27
2.1 preparation of pure bacterial suspensions
The purified strain was subjected to addition of additional carbon source (SMX for strain P27 and 3A5MI for strain N27) for R2A solid culture medium (peptone 0.5 g, yeast extract powder 0.5 g, casein hydrolysate 0.5 g, glucose 0.5 g, soluble starch 0.5 g, dipotassium hydrogen phosphate 0.3 g, anhydrous magnesium sulfate 0.024 g, sodium pyruvate 0.3 g, agar 15.0 g, distilled water 1000 mL) is activated, single colony is respectively picked to a sterilized inorganic salt culture medium, and the corresponding culture medium is addedCarbon source, in the 30 ℃, 150 rpm shaking culture. After the bacterial liquid is turbid, transferring the turbid bacterial liquid into a fresh inorganic culture medium by 10 percent of transfer amount, repeating the operation, and obtaining the pure bacterial seed liquid with stable degradation capability and growth cycle which can be used for analyzing the subsequent degradation efficiency.
2.2 analysis of the degradation efficiency of the strains P27 and N27 cultured alone on SMX and 3A5MI, respectively
Degradation of SMX by strain P27: adding a substrate SMX 50 mg/L in a 250 mL conical flask (containing 100 mL of sterilized inorganic salt culture medium) in the form of mother liquor, inoculating the prepared pure bacteria suspension in a transfer amount of 10%, adding a substrate SMX 50 mg/L in a 250 mL conical flask (containing 100 mL of sterilized inorganic salt culture medium) in the form of mother liquor, inoculating the prepared pure bacteria seed solution in a transfer amount of 10%, and performing shaking culture at a constant temperature of 30 ℃, wherein 3 times of treatment is set, and the culture medium without bacteria is used as a control. The biodegradation of the strain P27 on SMX is shown in the attached figure 5 (no effect of a non-biological control), and the measurement result shows that the strain P27 completely degrades 50 mg/L of SMX within 20 h and stably generates an equimolar nitrogen heterocyclic product 3A5 MI.
Degradation of 3A5MI by strain N27: adding the substrate 3A5MI 50 mg/L in the form of mother liquor into a 250 mL conical flask (containing 100 mL of sterilized inorganic salt culture medium), inoculating the prepared pure bacteria suspension in an amount of 10 percent of the transfer quantity, and performing shake culture at the constant temperature of 30 ℃, wherein 3 times of treatment is set, and the culture medium without bacteria is used as a control. The biodegradation of the strain N27 on 3A5MI is shown in figure 6 (no effect of a non-biological control), and the measurement result shows that the strain N27 can completely degrade 50 mg/L of 3A5MI within 40 h without product accumulation.
Example 3
Analysis of degradation substrate spectrum of sulfonamide antibiotics by strain P27
Substrate profiling of strain P27: the inoculation source adopts the pure bacterial suspension prepared in 2.1. In this stage, whether the strain P27 has the benzene ring side degradation capability on 9 other widely used sulfa antibiotics except SMX, including sulfapyridine, sulfamethazine, sulfamethizole, sulfachloropyridazine, sulfadimethoxine, sulfamethazine, 5-methoxypyrimidine, sulfadiazine and sulfamonomethoxine, is examined. Adding corresponding substrate in the form of mother liquor into a conical flask with the volume of 50 mL (containing 20 mL of sterilized inorganic salt culture medium) until the final concentration is 50 mg/L, inoculating the prepared strain P27 seed solution with the transfer amount of 10%, and performing shake culture at the constant temperature of 30 ℃, wherein 3 times of treatment is set for each treatment, and the culture medium without adding the strain is used as a control. The results show that as shown in fig. 7 (non-biological control has no effect), within 30.0 h, 39.8 h, 30.0 h, 53.8 h, 34.8 h, 30.0 h, 39.8 h and 30.0 h, the strain P27 can completely degrade sulfapyridine, sulfamethazine, sulfamethizole, sulfachloropyridazine, sulfadimethoxine, sulfamethazine, sulfanilamide-5-methoxypyrimidine, sulfadiazine and sulfamonomethoxine respectively to generate corresponding nitrogen heterocyclic products, and is accompanied with obvious biomass increase. These results indicate that the strain P27 has an extremely wide substrate spectrum, can effectively cope with the removal of various sulfonamides antibiotics in the environment, and has a great bioremediation potential.
Example 4
Degradation efficiency analysis of double-culture SMX benzene ring side degradation bacterium P27 and nitrogen heterocyclic product degradation bacterium N27
Analysis of degradation efficiency of synergistic degradation of SMX by strains P27 and N27: the inoculation source adopts the pure strain seed liquid prepared in the step 2.1. At the stage, the SMX synergistic degradation efficiency of the two strains under three concentration gradients of high, medium and low is investigated. Adding substrate SMX in a 250 mL conical flask (containing 100 mL of sterilized inorganic salt culture medium) in the form of mother liquor to the final concentrations of 1.04 mg/L, 5.88 mg/L and 53.02 mg/L respectively, inoculating the prepared pure strain seed liquid in 5% of transfer amount respectively, and performing shake culture at constant temperature of 30 ℃, wherein 3 times of treatment is set for each treatment, and the culture medium without strain is used as a control. The results are shown in fig. 8 (non-biological control has no effect), at three initial concentrations of 1.04 mg/L, 5.88 mg/L and 53.02 mg/L, the accumulation of 3A5MI, which originally occurs with the SMX degradation, disappears, especially at medium and low concentrations, 3A5MI is never detected during the degradation process, and the SMX removal rates of 87%, 100% and 100% can be achieved at 8 h, 14 h and 19 h, respectively. Therefore, under the condition of higher or lower initial concentration of SMX, the two bacteria can complete the complete removal of the SMX by a synergistic interaction mode. Compared with the existing biodegradation reports, the research greatly improves the degradation depth (complete mineralization) of the sulfonamide antibiotics, and 2 degradation bacteria obtained by separation provide important strain resources for the bioremediation of the sulfonamide antibiotics, and have good bioremediation application prospects.
The foregoing is a more detailed description of the invention in connection with specific preferred embodiments and it is not intended that the invention be limited to these specific details. For those skilled in the art to which the invention pertains, several simple deductions or substitutions can be made without departing from the spirit of the invention, and all shall be considered as belonging to the protection scope of the invention.
Sequence listing
<110> Shenzhen university of Harbin industry (Shenzhen) (Shenzhen institute of science and technology innovation, Harbin industry university)
<120> sulfonamide antibiotics synergistic degradation bacteria and application thereof
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Claims (7)
1. Sulfonamide antibiotics synergistically degrade bacteria, characterized in that they comprise:
arthrobacter paradonii (Paenarthrobactersp.) P27, deposited in the China general microbiological culture Collection center, with the preservation date: 12 months 03 days 2019; the preservation number is: CGMCC number 19070;
nocardioid bacterium (A), (B), (C), (Nocardioidessp.) N27, deposited in the China general microbiological culture Collection center, with the preservation date: 12 months 03 days 2019; the preservation number is: CGMCC number 19071.
2. Sulfonamide antibiotic co-degrading bacteria according to claim 1, characterized in that: the arthrobacter pseudolyticus P27 and the nocardioides pseudonocardioides N27 are separated from activated sludge of a sewage treatment plant.
3. Sulfonamide antibiotic co-degrading bacteria according to claim 1, characterized in that: the Arthrobacter pseudoacacia P27 takes SMX as a unique carbon source and energy source under aerobic conditions, is used for self growth and propagation by assimilating one side of a benzene ring of the SMX, and simultaneously stably accumulates a nitrogen heterocyclic product 3A5MI, and the Nocardia pseudoacacia N27 takes an SMX degradation product 3A5MI as a unique carbon source and energy source for self growth and propagation.
4. Sulfonamide antibiotic co-degrading bacteria according to claim 1, characterized in that: the sulfonamide antibiotics comprise at least one of sulfamethoxazole, sulfapyridine, sulfamethazine, sulfamethizole, sulfachloropyridazine, sulfadimethoxine, sulfamethazine, sulfanilamide-5-methoxypyrimidine, sulfadiazine and sulfamonomethoxine.
5. Use of a sulfonamide antibiotic to synergistically degrade bacteria according to claim 1, wherein: the bacteria for synergistically degrading the sulfonamide antibiotics are used for synergistically degrading the sulfonamide antibiotics.
6. Use of a sulfonamide antibiotic to synergistically degrade bacteria according to claim 5, wherein: the synergistic degradation bacteria of the sulfonamide antibiotics are applied to degradation of the sulfonamide antibiotics remained in sewage, wastewater, substrate and soil.
7. Use of a sulfonamide antibiotic to synergistically degrade bacteria according to claim 5, wherein: the environment for degradation is as follows: in the environment of inorganic salt, the temperature is 15-40 ℃, the initial pH is 5-9, and the environment is aerobic.
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