CN114455718B - Method for treating sulfanilamide high-salt wastewater by using bioengineering bacteria - Google Patents

Method for treating sulfanilamide high-salt wastewater by using bioengineering bacteria Download PDF

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CN114455718B
CN114455718B CN202111667183.9A CN202111667183A CN114455718B CN 114455718 B CN114455718 B CN 114455718B CN 202111667183 A CN202111667183 A CN 202111667183A CN 114455718 B CN114455718 B CN 114455718B
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宋少林
曾杲
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Jiangsu Hepu Environmental Protection Technology Co ltd
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Abstract

The invention relates to the technical field of wastewater treatment, and discloses a biological engineering bacteria treatment method for sulfonamide high-salt wastewater, which comprises the following steps: s1: screening according to the quality of the sulfonamide high-salt wastewater to obtain an MEFS engineering flora; s2: putting the MEFS engineering flora into the first wastewater for domestication and amplification; s3: putting the MEFS engineering flora obtained in the step S2 into second wastewater for fermentation pretreatment; s4: diluting the pretreated effluent, treating the effluent by a continuous two-stage AO biological treatment process, and then discharging the effluent after reaching the standard. The MEFS engineering flora adopted by the invention has unique advantages for treating the sulfonamide high-salt wastewater, and improves the biodegradability and the treatment efficiency of the high-concentration wastewater; the biological pretreatment is carried out on the high-concentration wastewater, the biological toxicity of the high-concentration wastewater is reduced, the load of a subsequent conventional system is reduced as much as possible, and the treatment cost is reduced by a conventional biological treatment process.

Description

Biological engineering bacteria treatment method for sulfonamide high-salt wastewater
Technical Field
The invention relates to the technical field of wastewater treatment, in particular to a biological engineering bacteria treatment method for sulfonamide high-salt wastewater.
Background
Sulfonamides (SAs) are a generic term for a class of drugs having a sulfanilamide structure, and are a class of chemotherapeutic drugs for the prevention and treatment of bacterial infectious diseases. The waste water generated in the production process of sulfonamides has the characteristics of high concentration, high toxicity, high salt content and difficult biodegradation, and the prior method for treating sulfanilamide waste water mainly comprises an adsorption method, a wet catalytic oxidation method, a Fenton method, an ozone oxidation method, a biodegradation method and the like. However, the conventional biochemical treatment of sulfanilamide wastewater is difficult, and development of a more efficient biological treatment process is urgently needed.
The 'engineering strain mixed fermentation' (MFES) technology is to screen high-efficiency stress-resistant strains aiming at pollutants in wastewater and to perform mixed fermentation by taking the wastewater as a culture medium. Unlike the conventional activated sludge process, organic matter and total nitrogen in wastewater are mainly formed by microbial fermentation through assimilation, and are discharged out of the system in the form of single cell protein, and a small amount of organic matter is consumed through respiration. During fermentation, the originally "low BOD" components, such as THF, are converted by a single engineered bacterial species into "high BOD" components, such as fatty acids, proteins, etc. Therefore, the COD of the wastewater treated by the process of 'engineering strain mixed fermentation' is greatly reduced, the B/C value is remarkably improved, and the process is more suitable for the treatment of a subsequent conventional system.
The Chinese invention patent with publication number CN112851045A discloses a sulfanilamide wastewater treatment system and a sulfanilamide wastewater treatment method, wherein the system comprises a regulation sedimentation tank, a filter, an absorber, an electrocatalytic oxidation device, a biological reaction area and a sedimentation tank II which are sequentially connected, and the filter is simultaneously communicated with a dosing device; the method comprises the following steps: introducing the sulfanilamide wastewater into an adjusting sedimentation tank for sedimentation, and carrying out homogenization and uniform treatment; adjusting the pH value; filtering; performing adsorption treatment and electrocatalytic oxidation treatment on the waste liquid by using a resin adsorber; carrying out anoxic biological reaction treatment on the obtained waste liquid, and then carrying out aerobic biological reaction treatment; and discharging after sedimentation and disinfection. The method has the disadvantages of low treatment efficiency, large treatment burden and high required cost when the conventional biochemical system is adopted to treat the high-concentration high-salinity wastewater.
Disclosure of Invention
In order to solve the technical problems, the invention provides a method for treating sulfanilamide type high-salt wastewater by using bioengineering bacteria, which utilizes specific flora obtained by screening and adopts an MFES technology to carry out biological pretreatment, thereby improving the treatment efficiency and reducing the treatment load of a subsequent conventional system.
The specific technical scheme of the invention is as follows: a biological engineering bacteria treatment method for sulfonamide high-salt wastewater comprises the following steps:
s1: screening according to the quality of the sulfonamide high-salt wastewater to obtain an MEFS engineering flora;
s2: putting the MEFS engineering flora into the first wastewater for domestication and amplification;
s3: putting the MEFS engineering flora obtained in the step S2 into second wastewater for fermentation pretreatment;
s4: diluting the pretreated effluent, and then treating the effluent in a continuous two-stage AO biological treatment process, and then discharging the effluent after reaching the standard;
the MEFS engineering flora comprises the following components: 40 to 60 percent ofPseudomonas alcaliphilaPseudomonas alcaligenes, 10 to 30 percentMicrobacterium lacticum30 to 50 percent of lactobacillus tenuisHalomonas hamiltoniiHalomonas handii.
The sulfanilamide high-salt wastewater contains a large amount of substances with biological inhibition or toxicity, even if the content of the substances is not large, the system is easy to be impacted, and the sulfanilamide high-salt wastewater is difficult to be decomposed and oxidized by microorganisms in a conventional biological treatment system. The MEFS engineering flora is screened for the water quality of the sulfonamide high-salt wastewater, and is obtained by screening and domesticating target degradation substances serving as main carbon sources and energy sources, so that the MEFS engineering flora has strong degradation capability on the target degradation substances. When the system is impacted by extreme conditions, such as operational errors and the like, which cause the impact of extreme factors such as high toxicity, extreme acid, extreme alkali and the like in the inlet water, the MEFS engineering flora can recover the efficient degradation function in a short time. Therefore, fermentation pretreatment of the MEFS engineering flora is used as a first barrier of the sewage treatment system, so that a conventional activated sludge system can be protected, and an early warning effect can be achieved.
The MEFS engineering flora which is screened and domesticated and amplified in the early stage is adopted to carry out biological pretreatment on the high-concentration wastewater, so that the biological toxicity of the high-concentration wastewater is reduced, the biodegradability of the high-concentration wastewater is improved, the load of a subsequent conventional system is reduced as much as possible, and the standard discharge of the wastewater is realized through a conventional biological treatment process. The invention can greatly reduce the treatment burden of a subsequent conventional biochemical system and has unique advantages for treating the sulfonamide high-salt wastewater. Meanwhile, the method has the advantages of low treatment cost, high treatment efficiency and good environmental protection benefit.
Preferably, the sulfanilamide type high-salt wastewater contains one or more of sulfanilamide, sulfachlorpyridazine sodium, sulfachlorpyridazine and sulfachlorpyridazine sodium.
Preferably, in S2, the salinity of the first wastewater ranges from 1 to 1.5%; and in S3, the salinity of the second wastewater is 1.5 to 2 percent.
Preferably, in S2, the domesticated amplification method comprises: putting MEFS engineering flora into the first wastewater for fermentation pretreatment, and treating pretreated effluent by a continuous AAO biological treatment process after hydrolysis acidification; after stable operation, an aerobic section is connected to the outlet water position at the tail end of the original process treatment, and the treatment is continued until stable operation.
Preferably, the markers of the domesticated amplification completion are: the COD of the effluent is stabilized below 200 mg/L, the total removal rate of the COD is stabilized above 95%, the ammonia nitrogen of the effluent is stabilized below 20 mg/L, and the total removal rate of the ammonia nitrogen is stabilized above 93%.
Preferably, in S3, the dilution is: mixing the pretreated effluent and low-concentration wastewater according to the ratio of 1 to 2 to 3, and adding water to dilute the salinity to 1 to 1.5%; the salinity of the low-concentration wastewater is 15000 to 18000mg/L, and the COD is 7000 to 8500mg/L.
Preferably, the carrier of the MEFS engineering flora is a modified hydrogel carrier, and the preparation method comprises the following steps:
ultrasonically dispersing nano ferroferric oxide particles in a cyclodextrin water solution, adding sodium alginate, stirring, sequentially performing magnetic attraction and filtration, and dissolving in a polyvinyl alcohol solution, wherein the mass ratio of the nano ferroferric oxide particles to the cyclodextrin to the sodium alginate to the polyvinyl alcohol is 1:1.8 to 3:2~9:8 to 20; and then, adding a crosslinking liquid, heating and mixing, pouring the obtained product into a mold, standing and cooling to form a hydrogel carrier with a smooth surface, spraying EVA emulsion on the hydrogel carrier, drying, and repeating 4~8 times to obtain the modified hydrogel carrier with a rough surface.
The invention takes polyvinyl alcohol as raw material to prepare biological hydrogel carrier, the polyvinyl alcohol is often used as substrate for preparing the microbial carrier because of the characteristics of innocuity, antimicrobial decomposition, high mechanical strength, low price and the like, and the polyvinyl alcohol forms a three-dimensional cross-linked network form of hydrogel, so that the specific surface area can be increased through water absorption and swelling, the strain loading rate is improved, and the biological treatment efficiency of wastewater is further improved.
The nano ferroferric oxide particles are magnetic particles, so that the convenience of recycling the carrier can be improved. However, the nano particles are easy to disperse unevenly to cause agglomeration, and the dispersion uniformity can be improved by processing the nano particles with cyclodextrin and sodium alginate and then blending the nano particles with polyvinyl alcohol. The cyclodextrin has good affinity with the nano ferroferric oxide particles, the hollow cylindrical structure of the cyclodextrin is favorable for forming an inclusion compound, and the nano ferroferric oxide particles are wrapped in a macromolecular chain by utilizing the adhesion of sodium alginate. Then, the internal cross-linking of the hydrogel can form a three-dimensional network structure among polyvinyl alcohol, cyclodextrin and sodium alginate, so that the compatibility of the nano particles and the overall combination stability are further improved, and the formed hydrogel carrier substance has good biocompatibility and can improve the affinity and the adsorbability with strains, so that the strain immobilization is better, and the fermentation treatment efficiency is higher.
In addition, the EVA coated on the surface of the hydrogel carrier can improve the impact resistance and the COD resistance, and has better adsorbability on sulfonamide wastewater. The hydrogel is dehydrated due to the heating treatment during surface coating, and the hydrogel surface is rough due to the partial crosslinking effect between the EVA and the polyvinyl alcohol matrix and the intermittent spraying means. The uneven texture is beneficial to improving the specific surface area of the hydrogel, particularly the increased surface area after the hydrogel absorbs water and swells is larger, the strain treatment efficiency in unit time is higher, and the treatment cost is reduced.
Preferably, the particle size of the nano ferroferric oxide particles is 20 to 45nm; the concentration of the cyclodextrin aqueous solution is 10 to 20 percent; the concentration of the polyvinyl alcohol solution is 8 to 15 percent; the polyvinyl alcohol has a molecular weight of 1.6 x 10 4 ~4*10 5 。
The particle size of the nano ferroferric oxide particles is controlled, so that the nano ferroferric oxide particles can be dispersed uniformly and have good magnetic adsorption performance. The concentration of the polyvinyl alcohol solution influences the dispersion degree of the nano particles, and the dispersion uniformity and compatibility within the range are better. The molecular weight of polyvinyl alcohol affects the viscosity and mechanical strength after cross-linking, and needs to be controlled in order to make the hydrogel carrier have better impact resistance and better strain immobilization.
Preferably, the crosslinking liquid is formaldehyde solution; the heating and mixing temperature is 40 to 80 ℃, and the time is 1 to 4 hours; the temperature of the vacuum drying is 60 to 90 ℃, the time is 10 to 35min, and the viscosity of the EVA emulsion is 200 to 500mPa.S.
When the viscosity of the EVA exceeds a limited range, the particle morphology and emulsion stability are poor, uneven spraying is easily caused, the combination stability of the modified hydrogel carrier is poor, and the impact resistance is also reduced.
Preferably, the emission compliance indicator is: COD is treated to be less than 300 mg/L, the total removal rate of COD is stabilized to be more than 96%, ammonia nitrogen is treated to be less than 10 mg/L, and the total removal rate of ammonia nitrogen is stabilized to be more than 97%.
Compared with the prior art, the invention has the following advantages:
(1) The MEFS engineering flora screened and domesticated and amplified in the early stage has unique advantages for treating the sulfonamide high-salt wastewater, and the biodegradability and the treatment efficiency of the high-concentration wastewater are improved;
(2) The high-concentration wastewater is subjected to biological pretreatment, the biological toxicity of the high-concentration wastewater is reduced, the load of a subsequent conventional system is reduced as much as possible, and the treatment cost is reduced by a conventional biological treatment process;
(3) The modified hydrogel carrier can improve the strain loading rate, optimize the stability and impact resistance of carrier loading, further improve the strain treatment efficiency and reduce the effluent COD value and ammonia nitrogen content.
Detailed Description
The present invention will be further described with reference to the following examples.
General examples
The sulfanilamide high-salt wastewater contains one or more of sulfanilamide, sulfachloropyridazine sodium, sulfachloropyrazine and sulfachloropyridazine sodium, and the bioengineering bacteria treatment method comprises the following steps:
s1: screening according to the main carbon source and energy of target degradation substances in the water quality of the sulfonamide high-salt wastewater to obtain MEFS engineering flora comprising 40-60 percent of target degradation substancesPseudomonas alcaliphilaPseudomonas alcaligenes, 10 to 30 percentMicrobacterium lacticum30 to 50 percent of lactobacillus fine powderHalomonas hamiltoniiHalomonas handii;
s2: throwing MEFS engineering flora into first wastewater with salinity of 1-1.5%, and performing fermentation pretreatment, wherein the inlet water concentration COD is more than 5000 mg/L;
after hydrolysis and acidification, the pretreated effluent enters a continuous AAO biological treatment process for treatment, so that the MEFS engineering flora is resistant to high salinity, and the detoxification effect of the pretreatment unit on the wastewater is simultaneously tested; the system continuously enters and exits water and stably operates, the COD of the discharged water is stabilized below 500mg/L, and the ammonia nitrogen is stabilized below 200 mg/L;
then, an aerobic section is connected to the outlet water position at the tail end of the original process treatment, the treatment is continued until the stable operation is achieved, the COD of the outlet water is stabilized below 200 mg/L, the total removal rate of the COD is stabilized above 95%, the ammonia nitrogen of the outlet water is stabilized below 20 mg/L, and the total removal rate of the ammonia nitrogen is stabilized above 93%;
s3: throwing the MEFS engineering flora obtained in the step S2 into second wastewater with the salinity of 1.5 to 2 percent for fermentation pretreatment; the COD of the inlet water of the pretreatment section is more than 6000 mg/L;
s4: diluting the pretreated effluent, mixing the effluent with low-concentration wastewater with salinity of 15000 to 18000mg/L, COD of 7000 to 8500mg/L according to the volume ratio of 1 to 3, and adding water to dilute the salinity to 1 to 1.5 percent; and then, the continuous two-stage AO biological treatment process is carried out for treatment, the treatment is stably carried out until the discharge reaches the standard, the COD treatment is below 300 mg/L, the total removal rate of the COD is stabilized above 96%, the ammonia nitrogen treatment is below 10 mg/L, and the total removal rate of the ammonia nitrogen is stabilized above 97%.
Wherein, the carrier of the MEFS engineering flora is a modified hydrogel carrier, and the preparation method comprises the following steps:
ultrasonically dispersing nano ferroferric oxide particles with the particle size of 20-45nm into a cyclodextrin water solution with the concentration of 10-20%, adding sodium alginate, stirring, sequentially carrying out magnetic attraction and filtration, and dissolving in a polyvinyl alcohol solution with the concentration of 8-15%, wherein the mass ratio of the nano ferroferric oxide particles to the cyclodextrin to the sodium alginate to the polyvinyl alcohol is 1:1.8 to 3:2~9:8 to 20, the molecular weight of the polyvinyl alcohol is 1.6 x 10 4 ~4*10 5 (ii) a Then adding a formaldehyde solution, heating and mixing for 1-4 h at 40-80 ℃, pouring the obtained product into a mold, standing and cooling to form a hydrogel carrier with a smooth surface; spraying EVA emulsion with viscosity of 200-500mPa.S, vacuum drying at 60-90 ℃ for 10-35min, and repeating 4~8 times to obtain the modified hydrogel carrier with rough surface.
Example 1
The sulfanilamide high-salt wastewater contains sulfanilamide, sulfachlorpyridazine sodium, sulfachlorpyridazine and sulfachlorpyridazine sodium, and the biological engineering bacteria treatment method comprises the following steps:
s1: according to the quality of sulfanilamide high-salt wastewaterThe target degradation substance is used as a main carbon source and an energy source to screen and obtain the MEFS engineering flora, which comprises 40 percent ofPseudomonas alcaliphilaPseudomonas alcaligenes, 30%Microbacterium lacticum30% of lactobacillusHalomonas hamiltoniiHalomonas handii;
s2: putting MEFS engineering flora into first wastewater with salinity of 1.5%, performing fermentation pretreatment, wherein the feed water concentration COD is 5300 mg/L, treating the feed water concentration to 1000 mg/L through a fermentation pretreatment section, the retention time is 6d, and the removal rate of COD is 81%;
after hydrolysis and acidification, the pretreated effluent enters a continuous AAO biological treatment process for treatment, so that the MEFS engineering flora is resistant to high salinity, and the detoxification effect of the pretreatment unit on the wastewater is simultaneously tested; the system continuously feeds in and discharges water and stably runs, the COD of the discharged water is 426 mg/L, and the ammonia nitrogen is 146 mg/L;
and then, an aerobic section is connected to the outlet water at the tail end of the original process treatment, the treatment is continued until the stable operation is realized, the COD of the outlet water is 177 mg/L, the total removal rate of the COD is 96.1%, the ammonia nitrogen of the outlet water is 17.6 mg/L, and the total removal rate of the ammonia nitrogen is stabilized at 93.9%.
S3: putting the MEFS engineering flora obtained in the S2 into second wastewater with the salinity of 2% for fermentation pretreatment; the pretreatment section averagely has 40.6L of inflow water per day, 6280 mg/L of inflow water, 1304 mg/L of outflow water, the average retention time of the pretreatment section is 8 d, and the removal rate of COD is 79.2 percent;
s4: diluting the pretreated effluent, mixing the pretreated effluent with low-concentration wastewater with the salinity of 16000mg/L, COD of 8000mg/L according to the volume ratio of 1:2, and adding water to dilute the salinity to 1.5%; and then, the continuous two-stage AO biological treatment process is carried out for treatment, the treatment is stably carried out until the discharge reaches the standard, the COD treatment reaches 293mg/L, the total removal rate of the COD is 96.1%, the ammonia nitrogen treatment reaches 4.1 mg/L, and the total removal rate of the ammonia nitrogen is 97.7%.
The MEFS engineering flora is a mixture of polyurethane filler and powdered activated carbon as a microbial carrier.
Example 2
The sulfanilamide, sulfachlorpyridazine and sulfachlorpyridazine sodium are contained in the sulfanilamide high-salt wastewater, and the biological engineering bacteria treatment method comprises the following steps:
s1: screening according to target degradation substances in the water quality of the sulfanilamide high-salt wastewater as main carbon sources and energy sources to obtain MEFS engineering flora containing 50 percent of target degradation substancesPseudomonas alcaliphilaPseudomonas alcaligenes, 20%Microbacterium lacticum 30% of lactobacillusHalomonas hamiltoniiHalomonas handii;
s2: putting MEFS engineering flora into first wastewater with salinity of 1.5%, performing fermentation pretreatment, wherein the feed water concentration COD is 5500 mg/L, the feed water concentration is treated to 1100 mg/L by a fermentation pretreatment section, the retention time is 6d, and the removal rate of COD is 80%;
after hydrolysis and acidification, the pretreated effluent enters a continuous AAO biological treatment process for treatment, so that the MEFS engineering flora is resistant to high salinity, and the detoxification effect of the pretreatment unit on the wastewater is simultaneously tested; the system continuously enters and exits water and stably operates, the COD of the effluent is 442 mg/L, and the ammonia nitrogen is 160 mg/L;
and then, an aerobic section is connected to the outlet at the tail end of the original process treatment, the treatment is continued until the stable operation is achieved, the COD of the outlet water is 180 mg/L, the total removal rate of the COD is 96.7%, the ammonia nitrogen of the outlet water is 17.1mg/L, and the total removal rate of the ammonia nitrogen is stabilized at 93.5%.
S3: throwing the MEFS engineering flora obtained in the S2 into second wastewater with the salinity of 2% for fermentation pretreatment; the pretreatment section averagely has 40.6L of inflow water per day, the inflow COD is 6351 mg/L, the outflow COD is 1413 mg/L, the average retention time of the pretreatment section is 8 d, and the COD removal rate is 77.8 percent;
s4: diluting the pretreated effluent, mixing the pretreated effluent with low-concentration wastewater with the salinity of 16000mg/L, COD of 8000mg/L according to the volume ratio of 1:2, and adding water to dilute the salinity to 1.5%; and then, the continuous two-stage AO biological treatment process is carried out for treatment, the treatment is stably carried out until the discharge reaches the standard, the COD treatment reaches 247 mg/L, the total removal rate of the COD is 96.3 percent, the ammonia nitrogen treatment reaches 4.9 mg/L, and the total removal rate of the ammonia nitrogen is 97.2 percent.
The MEFS engineering flora is a mixture of polyurethane filler and powdered activated carbon as a microbial carrier.
Example 3
The sulfanilamide high-salt wastewater contains sulfanilamide, sulfachlorpyridazine and sulfachlorpyridazine sodium, and the biological engineering bacteria treatment method comprises the following steps:
s1: screening according to the main carbon source and energy of target degradation substances in the water quality of the sulfonamide high-salt wastewater to obtain MEFS engineering flora containing 55 percent of target degradation substancesPseudomonas alcaliphilaPseudomonas alcaligenes, 10%Microbacterium lacticumLactobacillus, 35% ofHalomonas hamiltoniiHalomonas handii;
s2: putting MEFS engineering flora into first wastewater with salinity of 1.6%, performing fermentation pretreatment, wherein the feed water concentration COD is 5300 mg/L, treating the wastewater to 1093 mg/L through a fermentation pretreatment section, the retention time is 6d, and the removal rate of COD is 79.4%;
after hydrolysis and acidification, the pretreated effluent enters a continuous AAO biological treatment process for treatment, so that the MEFS engineering flora is resistant to high salinity, and the detoxification effect of the pretreatment unit on the wastewater is simultaneously tested; the system continuously enters and exits water and stably operates, the COD of the effluent is 455 mg/L, and the ammonia nitrogen is 163mg/L;
and then, an aerobic section is connected to the outlet at the tail end of the original process treatment, the treatment is continued until the stable operation is achieved, the COD of the outlet water is 182 mg/L, the total removal rate of the COD is 96.5%, the ammonia nitrogen of the outlet water is 16.9 mg/L, and the total removal rate of the ammonia nitrogen is stabilized at 93.8%.
S3: putting the MEFS engineering flora obtained in the S2 into second wastewater with the salinity of 2% for fermentation pretreatment; the pretreatment section averagely has 40.6L of inflow water per day, the inflow water COD is 6280 mg/L, the outflow water COD is 1476 mg/L, the average retention time of the pretreatment section is 8 d, and the COD removal rate is 76.5 percent;
s4: diluting the pretreated effluent, mixing the pretreated effluent with low-concentration wastewater with the salinity of 16000mg/L, COD of 8000mg/L according to the volume ratio of 1:2, and adding water to dilute the salinity to 1.6 percent; and then, carrying out treatment in a continuous two-stage AO biological treatment process, stably operating until the standard is reached and discharging, treating COD (chemical oxygen demand) to 269mg/L, wherein the total removal rate of COD is 96.2%, treating ammonia nitrogen to 4.8mg/L, and the total removal rate of ammonia nitrogen is 97.5%.
The MEFS engineering flora is a mixture of polyurethane filler and powdered activated carbon as a microbial carrier.
Example 4
The difference from example 1 is that: a modified hydrogel support is used.
The sulfanilamide high-salt wastewater contains sulfanilamide, sulfachlorpyridazine sodium, sulfachlorpyridazine and sulfachlorpyridazine sodium, and the biological engineering bacteria treatment method comprises the following steps:
s1: screening according to the main carbon source and energy of target degradation substances in the water quality of the sulfanilamide high-salt wastewater to obtain an MEFS engineering flora containing 40 percent of target degradation substancesPseudomonas alcaliphilaPseudomonas alcaligenes, 30%Microbacterium lacticum30% of lactobacillusHalomonas hamiltoniiHalomonas handii;
s2: throwing MEFS engineering flora into first wastewater with salinity of 1.5%, performing fermentation pretreatment, wherein the feed water concentration COD is 5300 mg/L, treating the wastewater to 956mg/L through a fermentation pretreatment section, the retention time is 6d, and the removal rate of COD is 82%;
after hydrolysis and acidification, the pretreated effluent enters a continuous AAO biological treatment process for treatment, so that the MEFS engineering flora is resistant to high salinity, and the detoxification effect of the pretreatment unit on the wastewater is simultaneously tested; the system continuously enters and exits water and stably operates, the COD of the effluent is 398 mg/L, and the ammonia nitrogen is 120 mg/L;
and then, an aerobic section is connected to the outlet at the tail end of the original process treatment, the treatment is continued until the stable operation is achieved, the COD of the outlet water is 161 mg/L, the total removal rate of the COD is 97.0%, the ammonia nitrogen of the outlet water is 15.3 mg/L, and the total removal rate of the ammonia nitrogen is stabilized at 94.7%.
S3: throwing the MEFS engineering flora obtained in the S2 into second wastewater with the salinity of 2% for fermentation pretreatment; the pretreatment section averagely has 40.6L of inflow water per day, 6280 mg/L of inflow water, 1257 mg/L of outflow water, the average retention time of the pretreatment section is 8 d, and the removal rate of COD is 80.0 percent;
s4: diluting the pretreated effluent, mixing the pretreated effluent with low-concentration wastewater with the salinity of 16000mg/L, COD of 8000mg/L according to the volume ratio of 1:2, and adding water to dilute the salinity to 1.5%; and then, the continuous two-stage AO biological treatment process is carried out for treatment, the treatment is stably carried out until the discharge reaches the standard, the COD treatment reaches 231 mg/L, the total removal rate of the COD is 96.9 percent, the ammonia nitrogen treatment reaches 3.5 mg/L, and the total removal rate of the ammonia nitrogen is 98.1 percent.
Wherein, the carrier of the MEFS engineering flora is a modified hydrogel carrier, and the preparation method comprises the following steps:
ultrasonically dispersing nano ferroferric oxide particles with the particle size of 30nm into a cyclodextrin water solution with the concentration of 10%, adding sodium alginate, stirring, sequentially performing magnetic attraction and filtration, and dissolving in a polyvinyl alcohol solution with the concentration of 11%, wherein the mass ratio of the nano ferroferric oxide particles to the cyclodextrin to the sodium alginate to the polyvinyl alcohol is 1:2.1:5:18, molecular weight of polyvinyl alcohol 3 x 10 5 (ii) a Then, adding formaldehyde solution, heating and mixing for 2h at 65 ℃, pouring the obtained product into a mold, standing and cooling to form a hydrogel carrier with a smooth surface; spraying EVA emulsion with viscosity of 386 mPa.S, vacuum drying at 75 deg.C for 22min, repeating for 5 times to obtain modified hydrogel carrier with rough surface.
Example 5
The difference from example 1 is that: a modified hydrogel support was used.
The sulfanilamide high-salt wastewater contains sulfanilamide, sulfachlorpyridazine sodium, sulfachlorpyridazine and sulfachlorpyridazine sodium, and the biological engineering bacteria treatment method comprises the following steps:
s1: screening according to target degradation substances in water quality of the sulfonamide high-salt wastewater as main carbon sources and energy sources to obtain MEFS engineering flora containing 40 percent of the target degradation substancesPseudomonas alcaliphilaPseudomonas alcaligenes, 30%Microbacterium lacticum30% of lactobacillusHalomonas hamiltoniiHalomonas handii;
s2: putting MEFS engineering flora into first wastewater with salinity of 1.5%, performing fermentation pretreatment, wherein the feed water concentration COD is 5300 mg/L, the feed water concentration is treated to 964mg/L by a fermentation pretreatment section, the retention time is 6d, and the removal rate of COD is 81.8%;
after hydrolysis and acidification, the pretreated effluent enters a continuous AAO biological treatment process for treatment, so that the MEFS engineering flora is resistant to high salinity, and the detoxification effect of the pretreatment unit on the wastewater is tested; the system continuously feeds in and discharges water and stably runs, the COD of the discharged water is 402 mg/L, and the ammonia nitrogen is 133 mg/L;
and then, an aerobic section is connected to the outlet at the tail end of the original process treatment, the treatment is continued until the stable operation is achieved, the COD of the outlet water is 164 mg/L, the total removal rate of the COD is 96.9%, the ammonia nitrogen of the outlet water is 15.7 mg/L, and the total removal rate of the ammonia nitrogen is stabilized at 94.6%.
S3: putting the MEFS engineering flora obtained in the S2 into second wastewater with the salinity of 2% for fermentation pretreatment; the pretreatment section averagely has 40.6L of inflow water per day, the inflow water COD is 6280 mg/L, the outflow water COD is 1280 mg/L, the average retention time of the pretreatment section is 8 d, and the COD removal rate is 79.6 percent;
s4: diluting the pretreated effluent, mixing the pretreated effluent with low-concentration wastewater with the salinity of 16000mg/L, COD of 8000mg/L according to the volume ratio of 1:2, and adding water to dilute the salinity to 1.5%; and then, the wastewater enters a continuous two-stage AO biological treatment process for treatment, the wastewater is stably operated until the wastewater reaches the discharge standard, COD is treated to 247 mg/L, the total removal rate of COD is 96.7%, ammonia nitrogen is treated to 3.8 mg/L, and the total removal rate of ammonia nitrogen is 97.9%.
The MEFS engineering flora carrier is a modified hydrogel carrier, and the preparation method comprises the following steps:
ultrasonically dispersing nano ferroferric oxide particles with the particle size of 35nm into a cyclodextrin water solution with the concentration of 17%, adding sodium alginate, stirring, sequentially performing magnetic attraction and filtration, and dissolving into a polyvinyl alcohol solution with the concentration of 13%, wherein the mass ratio of the nano ferroferric oxide particles to the cyclodextrin to the sodium alginate to the polyvinyl alcohol is 1:2.6:8:15, molecular weight of polyvinyl alcohol 2 x 10 5 (ii) a Then, adding formaldehyde solution, heating and mixing for 4h at 55 ℃, pouring the obtained product into a mold, standing and cooling to form a hydrogel carrier with a smooth surface; after spraying EVA emulsion with viscosity of 273 mPa.S, vacuum drying at 90 deg.C for 10min, repeating for 6 times to obtain modified hydrogel carrier with rough surface.
Comparative example 1
The difference from example 4 is that: the polyvinyl alcohol has a molecular weight of 1.4 x 10 4 。
The sulfanilamide high-salt wastewater contains sulfanilamide, sulfachlorpyridazine sodium, sulfachlorpyridazine and sulfachlorpyridazine sodium, and the biological engineering bacteria treatment method comprises the following steps:
s1: screening according to target degradation substances in water quality of the sulfonamide high-salt wastewater as main carbon sources and energy sources to obtain MEFS engineering flora containing 40 percent of the target degradation substancesPseudomonas alcaliphilaPseudomonas alcaligenes, 30%Microbacterium lacticum30% of lactobacillusHalomonas hamiltoniiHalomonas handii;
s2: putting MEFS engineering flora into first wastewater with salinity of 1.5%, performing fermentation pretreatment, wherein the feed water concentration COD is 5300 mg/L, the feed water concentration is treated to 1209mg/L by a fermentation pretreatment section, the retention time is 6d, and the removal rate of COD is 77%;
after hydrolysis and acidification, the pretreated effluent enters a continuous AAO biological treatment process for treatment, so that the MEFS engineering flora is resistant to high salinity, and the detoxification effect of the pretreatment unit on the wastewater is simultaneously tested; the system continuously feeds in and discharges water and stably operates, the COD of the discharged water is 501 mg/L, and the ammonia nitrogen is 189 mg/L;
and then, an aerobic section is connected to the outlet water at the tail end of the original process treatment, the treatment is continued until the stable operation is realized, the COD of the outlet water is 221mg/L, the total removal rate of the COD is 95.8%, the ammonia nitrogen of the outlet water is 18.3 mg/L, and the total removal rate of the ammonia nitrogen is stabilized at 93.6%.
S3: putting the MEFS engineering flora obtained in the S2 into second wastewater with the salinity of 2% for fermentation pretreatment; the pretreatment section averagely has 40.6L of inflow water per day, the inflow water COD is 6280 mg/L, the outflow water COD is 1432mg/L, the average retention time of the pretreatment section is 8 d, and the COD removal rate is 77.2 percent;
s4: diluting the pretreated effluent, mixing the pretreated effluent with low-concentration wastewater with the salinity of 16000mg/L, COD of 8000mg/L according to the volume ratio of 1:2, and adding water to dilute the salinity to 1.5%; and then, carrying out treatment in a continuous two-stage AO biological treatment process, stably running until the discharge reaches the standard, treating COD (chemical oxygen demand) to 284mg/L, wherein the total removal rate of the COD is 96.2%, treating ammonia nitrogen to 5.1mg/L, and the total removal rate of the ammonia nitrogen is 97.1%.
Wherein, the carrier of the MEFS engineering flora is a modified hydrogel carrier, and the preparation method comprises the following steps:
ultrasonically dispersing nano ferroferric oxide particles with the particle size of 30nm into cyclodextrin water solution with the concentration of 10%, adding sodium alginate, stirring, sequentially performing magnetic attraction and filtration, and dissolving into polyvinyl alcohol solution with the concentration of 11%, wherein the mass ratio of the nano ferroferric oxide particles to the cyclodextrin to the sodium alginate to the polyvinyl alcohol is 1:2.1:5:18, molecular weight of polyvinyl alcohol 1.4 x 10 4 (ii) a Then, adding formaldehyde solution, heating and mixing for 2 hours at 65 ℃, pouring the obtained product into a mold, standing and cooling to form a hydrogel carrier with a smooth surface; spraying EVA emulsion with viscosity of 386 mPa.S, vacuum drying at 75 deg.C for 22min, repeating for 5 times to obtain modified hydrogel carrier with rough surface.
Comparative example 2
The difference from example 4 is that: the polyvinyl alcohol has a molecular weight of 7 x 10 5 。
The sulfanilamide high-salt wastewater contains sulfanilamide, sulfachlorpyridazine sodium, sulfachlorpyridazine and sulfachlorpyridazine sodium, and the biological engineering bacteria treatment method comprises the following steps:
s1: screening according to target degradation substances in water quality of the sulfonamide high-salt wastewater as main carbon sources and energy sources to obtain MEFS engineering flora containing 40 percent of the target degradation substancesPseudomonas alcaliphilaPseudomonas alcaligenes, 30%Microbacterium lacticum30% of lactobacillusHalomonas hamiltoniiHalomonas handii;
s2: throwing the MEFS engineering flora into first wastewater with the salinity of 1.5 percent for fermentation pretreatment, wherein the inlet water concentration COD is 5300 mg/L, the inlet water concentration is treated to 1195mg/L through a fermentation pretreatment section, the retention time is 6d, and the COD removal rate is 77 percent;
after hydrolysis and acidification, the pretreated effluent enters a continuous AAO biological treatment process for treatment, so that the MEFS engineering flora is resistant to high salinity, and the detoxification effect of the pretreatment unit on the wastewater is simultaneously tested; the system continuously enters and exits water and stably operates, the COD of the effluent is 488 mg/L, and the ammonia nitrogen is 179mg/L;
and then, an aerobic section is connected to the outlet at the tail end of the original process treatment, the treatment is continued until the stable operation is realized, the COD of the outlet water is 239mg/L, the total removal rate of the COD is 95.5%, the ammonia nitrogen of the outlet water is 17.6 mg/L, and the total removal rate of the ammonia nitrogen is stabilized at 93.9%.
S3: putting the MEFS engineering flora obtained in the S2 into second wastewater with the salinity of 2% for fermentation pretreatment; the pretreatment section averagely has 40.6L of inflow water per day, 6280 mg/L of inflow water, 1441mg/L of outflow water COD, the average retention time of the pretreatment section is 8 d, and the COD removal rate is 77.1 percent;
s4: diluting the pretreated effluent, mixing the pretreated effluent with low-concentration wastewater with the salinity of 16000mg/L, COD of 8000mg/L according to the volume ratio of 1:2, and adding water to dilute the salinity to 1.5%; and then, performing treatment in a continuous two-stage AO biological treatment process, stably operating until the standard is reached, and discharging, wherein the COD is treated to 293mg/L, the total removal rate of the COD is 96.1%, the ammonia nitrogen is treated to 5.5mg/L, and the total removal rate of the ammonia nitrogen is 96.9%.
Wherein, the carrier of the MEFS engineering flora is a modified hydrogel carrier, and the preparation method comprises the following steps:
ultrasonically dispersing nano ferroferric oxide particles with the particle size of 30nm into a cyclodextrin water solution with the concentration of 10%, adding sodium alginate, stirring, sequentially performing magnetic attraction and filtration, and dissolving in a polyvinyl alcohol solution with the concentration of 11%, wherein the mass ratio of the nano ferroferric oxide particles to the cyclodextrin to the sodium alginate to the polyvinyl alcohol is 1:2.1:5:18, molecular weight of polyvinyl alcohol 7 x 10 5 (ii) a Then, adding formaldehyde solution, heating and mixing for 2h at 65 ℃, pouring the obtained product into a mold, standing and cooling to form a hydrogel carrier with a smooth surface; spraying EVA emulsion with viscosity of 386 mPa.S, vacuum drying at 75 deg.C for 22min, repeating for 5 times to obtain modified hydrogel carrier with rough surface.
Comparative example 3
The difference from example 4 is that: the viscosity of the EVA emulsion is 180 mPa.S.
The sulfanilamide high-salt wastewater contains sulfanilamide, sulfachlorpyridazine sodium, sulfachlorpyridazine and sulfachlorpyridazine sodium, and the biological engineering bacteria treatment method comprises the following steps:
s1: according to the target reduction in the water quality of the sulfonamide high-salt wastewaterScreening to obtain MEFS engineering flora containing 40% of decomposed substances as main carbon source and energy sourcePseudomonas alcaliphilaPseudomonas alcaligenes, 30%Microbacterium lacticum30% of lactobacillusHalomonas hamiltoniiHalomonas handii;
s2: putting MEFS engineering flora into first wastewater with salinity of 1.5%, performing fermentation pretreatment, wherein the feed water concentration COD is 5300 mg/L, the feed water concentration is treated to 1117mg/L by a fermentation pretreatment section, the retention time is 6d, and the removal rate of COD is 80%;
after hydrolysis and acidification, the pretreated effluent enters a continuous AAO biological treatment process for treatment, so that the MEFS engineering flora is resistant to high salinity, and the detoxification effect of the pretreatment unit on the wastewater is simultaneously tested; the system continuously enters and exits water and stably operates, the COD of the effluent is 431 mg/L, and the ammonia nitrogen is 150mg/L;
and then, an aerobic section is connected to the outlet at the tail end of the original process treatment, the treatment is continued until the stable operation is achieved, the COD of the outlet water is 184mg/L, the total removal rate of the COD is 96.5%, the ammonia nitrogen of the outlet water is 16.6 mg/L, and the total removal rate of the ammonia nitrogen is stabilized at 94.2%.
S3: putting the MEFS engineering flora obtained in the S2 into second wastewater with the salinity of 2% for fermentation pretreatment; the pretreatment section averagely has 40.6L of inflow water per day, 6280 mg/L of inflow water, 1301mg/L of outflow water COD, 8 d of average retention time of the pretreatment section and 79.4 percent of COD removal rate;
s4: diluting the pretreated effluent, mixing the pretreated effluent with low-concentration wastewater with the salinity of 16000mg/L, COD of 8000mg/L according to the volume ratio of 1:2, and adding water to dilute the salinity to 1.5%; and then, treating the wastewater by a continuous two-stage AO biological treatment process, stably running until the wastewater reaches the standard and is discharged, treating the wastewater by COD (chemical oxygen demand) to 263mg/L, wherein the total removal rate of the COD is 96.5%, treating the ammonia nitrogen to 4.6mg/L, and the total removal rate of the ammonia nitrogen is 97.5%.
The MEFS engineering flora carrier is a modified hydrogel carrier, and the preparation method comprises the following steps:
ultrasonically dispersing nano ferroferric oxide particles with the particle size of 30nm into cyclodextrin water solution with the concentration of 10%, adding sodium alginate, stirring, and dissolving in polyethylene with the concentration of 11% after magnetic attraction and filtration in sequenceIn the enol solution, the mass ratio of the nano ferroferric oxide particles, the cyclodextrin, the sodium alginate and the polyvinyl alcohol is 1:2.1:5:18, molecular weight of polyvinyl alcohol 3 x 10 5 (ii) a Then, adding formaldehyde solution, heating and mixing for 2h at 65 ℃, pouring the obtained product into a mold, standing and cooling to form a hydrogel carrier with a smooth surface; spraying EVA emulsion with viscosity of 180 mPa.S, vacuum drying at 75 deg.C for 22min, repeating for 5 times to obtain modified hydrogel carrier with rough surface.
Comparative example 4
The difference from example 4 is that: the viscosity of the EVA emulsion is 850 mPa.S.
The sulfanilamide high-salt wastewater contains sulfanilamide, sulfachloropyridazine sodium, sulfachloropyrazine and sulfachloropyridazine sodium, and the biological engineering bacteria treatment method comprises the following steps:
s1: screening according to target degradation substances in water quality of the sulfonamide high-salt wastewater as main carbon sources and energy sources to obtain MEFS engineering flora containing 40 percent of the target degradation substancesPseudomonas alcaliphilaPseudomonas alcaligenes, 30%Microbacterium lacticum30% of lactobacillusHalomonas hamiltoniiHalomonas handii;
s2: putting MEFS engineering flora into first wastewater with salinity of 1.5%, performing fermentation pretreatment, wherein the feed water concentration COD is 5300 mg/L, the feed water concentration is 1125mg/L after the treatment of a fermentation pretreatment section, the retention time is 6d, and the removal rate of COD is 80%;
after hydrolysis and acidification, the pretreated effluent enters a continuous AAO biological treatment process for treatment, so that the MEFS engineering flora is resistant to high salinity, and the detoxification effect of the pretreatment unit on the wastewater is simultaneously tested; the system continuously enters and exits water and stably operates, the COD of the effluent is 458 mg/L, and the ammonia nitrogen is 163mg/L;
and then, an aerobic section is connected to the outlet water position at the tail end of the original process treatment, the treatment is continued until the stable operation is realized, the COD of the outlet water is 192 mg/L, the total removal rate of the COD is 96.6 percent, the ammonia nitrogen of the outlet water is 16.8 mg/L, and the total removal rate of the ammonia nitrogen is stabilized at 94.2 percent.
S3: putting the MEFS engineering flora obtained in the S2 into second wastewater with the salinity of 2% for fermentation pretreatment; the pretreatment section averagely has 40.6L of inflow water per day, 6280 mg/L of inflow water, 1357mg/L of outflow water COD, the average retention time of the pretreatment section is 8 d, and the COD removal rate is 79.1 percent;
s4: diluting the pretreated effluent, mixing the pretreated effluent with low-concentration wastewater with the salinity of 16000mg/L, COD of 8000mg/L according to the volume ratio of 1:2, and adding water to dilute the salinity to 1.5%; and then, carrying out treatment in a continuous two-stage AO biological treatment process, stably running until the standard is reached and discharging, treating COD to 293mg/L, wherein the total removal rate of COD is 96.1%, treating ammonia nitrogen to 4.8mg/L, and the total removal rate of ammonia nitrogen is 97.3%.
The MEFS engineering flora carrier is a modified hydrogel carrier, and the preparation method comprises the following steps:
ultrasonically dispersing nano ferroferric oxide particles with the particle size of 30nm into cyclodextrin water solution with the concentration of 10%, adding sodium alginate, stirring, sequentially performing magnetic attraction and filtration, and dissolving into polyvinyl alcohol solution with the concentration of 11%, wherein the mass ratio of the nano ferroferric oxide particles to the cyclodextrin to the sodium alginate to the polyvinyl alcohol is 1:2.1:5:18, molecular weight of polyvinyl alcohol 3 x 10 5 (ii) a Then, adding formaldehyde solution, heating and mixing for 2h at 65 ℃, pouring the obtained product into a mold, standing and cooling to form a hydrogel carrier with a smooth surface; spraying EVA emulsion with viscosity of 850 mPa.S, vacuum drying at 75 deg.C for 22min, repeating for 5 times to obtain modified hydrogel carrier with rough surface.
Comparative example 5
The difference from example 4 is that: EVA emulsion is not sprayed in the preparation of the modified hydrogel carrier.
The sulfanilamide high-salt wastewater contains sulfanilamide, sulfachlorpyridazine sodium, sulfachlorpyridazine and sulfachlorpyridazine sodium, and the biological engineering bacteria treatment method comprises the following steps:
s1: screening according to target degradation substances in water quality of the sulfonamide high-salt wastewater as main carbon sources and energy sources to obtain MEFS engineering flora containing 40 percent of the target degradation substancesPseudomonas alcaliphilaPseudomonas alcaligenes, 30%Microbacterium lacticum30% of lactobacillusHalomonas hamiltoniiHan's capsuleHalomonas sp.salina;
s2: putting MEFS engineering flora into first wastewater with salinity of 1.5%, performing fermentation pretreatment, wherein the feed water concentration COD is 5300 mg/L, the feed water concentration is treated to 1005mg/L by a fermentation pretreatment section, the retention time is 6d, and the removal rate of COD is 81%;
after hydrolysis and acidification, the pretreated effluent enters a continuous AAO biological treatment process for treatment, so that the MEFS engineering flora is resistant to high salinity, and the detoxification effect of the pretreatment unit on the wastewater is simultaneously tested; the system continuously enters and exits water and stably operates, the COD of the effluent is 417mg/L, and the ammonia nitrogen is 140 mg/L;
and then, an aerobic section is connected to the outlet at the tail end of the original process treatment, the treatment is continued until the stable operation is achieved, the COD of the outlet water is 175mg/L, the total removal rate of the COD is 96.7%, the ammonia nitrogen of the outlet water is 16.0 mg/L, and the total removal rate of the ammonia nitrogen is stabilized at 94.4%.
S3: putting the MEFS engineering flora obtained in the S2 into second wastewater with the salinity of 2% for fermentation pretreatment; the pretreatment section averagely has 40.6L of inflow water per day, 6280 mg/L of inflow water, 1304 mg/L of outflow water, the average retention time of the pretreatment section is 8 d, and the removal rate of COD is 79.2 percent;
s4: diluting the pretreated effluent, mixing the pretreated effluent with low-concentration wastewater with the salinity of 16000mg/L, COD of 8000mg/L according to the volume ratio of 1:2, and adding water to dilute the salinity to 1.5%; and then, the wastewater enters a continuous two-stage AO biological treatment process for treatment, the treatment is stably carried out until the wastewater reaches the discharge standard, the COD is treated to 248 mg/L, the total removal rate of the COD is 96.7 percent, the ammonia nitrogen is treated to 3.7mg/L, and the total removal rate of the ammonia nitrogen is 97.9 percent.
Wherein, the carrier of the MEFS engineering flora is a modified hydrogel carrier, and the preparation method comprises the following steps:
ultrasonically dispersing nano ferroferric oxide particles with the particle size of 30nm into a cyclodextrin water solution with the concentration of 10%, adding sodium alginate, stirring, sequentially performing magnetic attraction and filtration, and dissolving in a polyvinyl alcohol solution with the concentration of 11%, wherein the mass ratio of the nano ferroferric oxide particles to the cyclodextrin to the sodium alginate to the polyvinyl alcohol is 1:2.1:5:18, molecular weight of polyvinyl alcohol 3 x 10 5 (ii) a Then, formaldehyde solution is added and heated and mixed for 2h at 65 ℃, and thenThe obtained substance is poured into a mould, and the hydrogel carrier with smooth surface is formed after standing and cooling.
In summary, from the examples 1 to 3, it can be seen that, when the salinity of the sulfonamide wastewater is controlled to be 1.5% to 2%, the wastewater after the MEFS engineering bacteria fermentation pretreatment is mixed with the low-concentration wastewater, and then the wastewater enters the conventional biochemical system for treatment, so that the wastewater can reach the discharge standard. The treatment method improves the biodegradability and the treatment efficiency of the high-concentration wastewater, reduces the load of a subsequent conventional system as much as possible, and reduces the subsequent treatment cost. In addition, the data of examples 4-5 show that the modified hydrogel carrier can improve the strain loading rate, optimize the stability and impact resistance of carrier loading, further improve the strain treatment efficiency and reduce the COD value and ammonia nitrogen content of effluent.
Further, with reference to example 4 and comparative examples 1-2, the impact resistance of the hydrogel carrier formed is significantly reduced due to the excessively low molecular weight of the polyvinyl alcohol, and the wastewater treatment efficiency is not high; when the molecular weight of the polyvinyl alcohol is too high, the viscosity is too high, agglomeration is easily caused, the dispersibility in the wastewater is poor, and the wastewater treatment efficiency is reduced. In combination with example 4 and comparative examples 3 to 4, when the viscosity of EVA was too low, the adhesion of the hydrogel carrier was poor, and the impact resistance was lowered for a long period of time, resulting in a higher COD value of the final effluent; the emulsion form instability when the viscosity of the EVA is too high is high, so that uneven spraying is easily caused, the combination stability of the modified hydrogel carrier is poor, and the impact resistance is reduced. By combining the embodiment 4 and the comparative example 5, the strain loading rate of the hydrogel carrier without spraying the EVA emulsion is reduced, the strain fermentation accommodating space is reduced, and the wastewater treatment efficiency is reduced.
The raw materials and equipment used in the invention are common raw materials and equipment in the field if not specified; the methods used in the present invention are conventional in the art unless otherwise specified.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and all simple modifications, alterations and equivalents of the above embodiments according to the technical spirit of the present invention are still within the protection scope of the technical solution of the present invention.

Claims (9)

1. A biological engineering bacteria treatment method for sulfonamide high-salt wastewater is characterized by comprising the following steps:
s1: screening according to the quality of the sulfanilamide high-salt wastewater to obtain an MFES engineering flora;
s2: putting the MFES engineering flora into the first wastewater for acclimatization and amplification;
s3: putting the MFES engineering flora obtained in the S2 into second wastewater for fermentation pretreatment;
s4: diluting the pretreated effluent, and then treating the effluent in a continuous two-stage AO biological treatment process, and then discharging the effluent after reaching the standard;
the MFES engineering flora comprises the following components: 40 to 60 percent ofPseudomonas alcaliphilaPseudomonas alcaligenes, 10 to 30 percentMicrobacterium lacticum30 to 50 percent of lactobacillus fine powderHalomonas hamiltoniiHalomonas handii;
the carrier of the MFES engineering flora is a modified hydrogel carrier, and the preparation method comprises the following steps:
ultrasonically dispersing nano ferroferric oxide particles in a cyclodextrin water solution, adding sodium alginate, stirring, sequentially magnetically attracting, filtering and dissolving in a polyvinyl alcohol solution, wherein the mass ratio of the nano ferroferric oxide particles to the cyclodextrin to the sodium alginate to the polyvinyl alcohol is 1:1.8 to 3:2~9:8 to 20; then, adding a crosslinking liquid, heating and mixing, pouring the obtained product into a mold, standing and cooling to form a hydrogel carrier with a smooth surface; and spraying EVA emulsion on the carrier, drying in vacuum, and repeating for 4~8 times to obtain the modified hydrogel carrier with rough surface.
2. The method for treating the sulfanilamide high-salt wastewater by using the bioengineering bacteria as claimed in claim 1, wherein the sulfanilamide high-salt wastewater contains one or more of sulfanilamide, sulfachloropyridazine, sulfachlorpyridazine sodium, sulfachloropyrazine and sulfachlorpyridazine sodium.
3. The method for treating sulfanilamide high-salt wastewater by using bioengineering bacteria as claimed in claim 1, wherein in S2, the salinity of the first wastewater is 1 to 1.5%; and in S3, the salinity of the second wastewater is 1.5 to 2 percent.
4. The method for treating sulfanilamide high-salt wastewater biological engineering bacteria according to claim 1 or 3, wherein in S2, the domestication and amplification method is as follows: putting the MFES engineering flora into the first wastewater for fermentation pretreatment, and treating pretreated effluent by a continuous AAO biological treatment process after hydrolysis acidification; after stable operation, an aerobic section is connected to the tail end effluent of the original process treatment, and the treatment is continued until stable operation.
5. The method for treating sulfanilamide high-salt wastewater by using bioengineering bacteria according to claim 4, wherein the acclimated amplification completion mark is as follows: the COD of the effluent is stabilized below 200 mg/L, the total removal rate of the COD is stabilized above 95%, the ammonia nitrogen of the effluent is stabilized below 20 mg/L, and the total removal rate of the ammonia nitrogen is stabilized above 93%.
6. The method for treating sulfanilamide high-salt wastewater by using bioengineering bacteria as claimed in claim 1 or 3, wherein in S4, the dilution is as follows: mixing the pretreated effluent with low-concentration wastewater according to the ratio of 1 to 2 to 3, and adding water to dilute the mixture to the salinity of 1 to 1.5 percent; the salinity of the low-concentration wastewater is 15000 to 18000mg/L, and the COD is 7000 to 8500mg/L.
7. The method for treating sulfanilamide high-salt wastewater by using bioengineering bacteria according to claim 1, wherein the particle size of the nano ferroferric oxide particles is 20 to 45nm; the concentration of the cyclodextrin aqueous solution is 10 to 20 percent; the concentration of the polyvinyl alcohol solution is 8 to 15 percent; the polyvinyl alcohol has a molecular weight of 1.6 x 10 4 ~4*10 5 。
8. The method for treating sulfanilamide high-salt wastewater by using bioengineering bacteria according to claim 7, wherein the crosslinking liquid is formaldehyde solution; the heating and mixing temperature is 40 to 80 ℃, and the time is 1 to 4 hours; the temperature of the vacuum drying is 60 to 90 ℃, and the time is 10 to 35min; the viscosity of the EVA emulsion is 200 to 500mPa.S.
9. The method for treating sulfanilamide high-salt wastewater by using bioengineering bacteria according to claim 1, wherein the standard discharge mark is: COD is treated to be less than 300 mg/L, the total removal rate of COD is stabilized to be more than 96%, ammonia nitrogen is treated to be less than 10 mg/L, and the total removal rate of ammonia nitrogen is stabilized to be more than 97%.
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