CN105645598A - Method and device for biologically degrading SMX (sulfamethoxazole) - Google Patents
Method and device for biologically degrading SMX (sulfamethoxazole) Download PDFInfo
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Abstract
The invention discloses a method and a device for biologically degrading SMX (sulfamethoxazole). The method comprises steps as follows: (1) performing seed culture to obtain secondary seeds of alcaligenes faecalis and pseudomonas sp. denitrificans; (2) preparing a degradation liquid; (3) containing the degradation liquid into two fermentation tanks, and inoculating two fermentation tanks with secondary seeds of alcaligenes faecalis and pseudomonas sp. denitrificans for fermentation; (4) filtering two fermentation broths with a hollow fiber membrane module, exchanging the fermentation tanks for fermentation filtrates for 2-3 h continuously, and storing the fermentation filtrates into two storage tanks respectively; (5) washing filtered alcaligenes faecalis back into respective fermentation tanks for fermentation. According to the device and the method, two bacterial cells are not contacted, so that low SMX degradation rates caused by inhibition of biomass due to contact of two bacterial cells are avoided. Meanwhile, the hollow fiber membrane system can promote the biomass of the two bacteria and inhibit re-synthesis of SMX.
Description
Technical field
The invention belongs to field of environment pollution control, relate to method and the device of a kind of biodegradation sulfamethoxazole.
Background technology
Sulfamethoxazole (SMX) is one of sulfa antibiotics of a kind of general prescription, consumption. Can detect that in waste water that SMX is at concentrations up to mg/L level, in the water of surface, content is ng/L level, even at 410ng/L being detected in subsoil water. Its existence in water environment can cause that serious water pollutes, and threatens the existence of water plant, animal and microorganism. Research finds that SMX could alter that the composition of microorganism species, causes the diffusion of resistant gene, and aquatile has mutagenicity or vegetalitas poison. Many bibliographical information biodegradations are the sewage disposal SMX topmost approach got rid of, and have studied some main bacterial strains with SMX degradation capability of the efficiency of its eliminating isolation identification. Although some relate to its biodegradable specific enzyme, degradation pathway, possible metabolite are set forth to some extent, but SMX biodegradation is still not very clear.
Membrane bioreactor (MBR) technology has been widely used in sewage disposal chemical pollutant. Influence factor's (such as sludge retention time, mixed liquor concentration of suspension, raw sewage capacity, oxidizing process, Soluble cercaria antigen etc.) of the treatment effeciency of Membrane Bioreactor for Wastewater Treatment organic pollution, film dirty and biological existence ability mainly studied by many documents. But the bibliographical information currently with membrane bioreactor degraded Antibiotique composition is not as many.
Single bacterium antibiotic ability of degrading is very limited, and if, with mutually promoting between mixed bacterium, the relation of Mutualism may improve the degraded of SMX. But finding in research before, the ability of Alcaligenesfaecalis and Pseudomonassp.denitrificans mixed bacterium degradation SMX is not so good as the mono-bacterium of Alcaligenesfaecalis. The research utilizing membrane bioreactor mixed bacterium degradation SMX is not yet reported.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that a kind of method of biodegradation sulfamethoxazole.
Second purpose of the present invention is to provide the device of a kind of biodegradation sulfamethoxazole.
Technical scheme is summarized as follows:
A kind of method of biodegradation sulfamethoxazole, comprises the steps:
(1) seed culture: the Bacillus foecalis alkaligenes (Alcaligenesfaecalis) that deposit number is CGMCCNo.1.767 is cultivated in seed culture medium, obtains the first order seed of Bacillus foecalis alkaligenes; The first order seed of Bacillus foecalis alkaligenes is cultivated in seed culture medium, obtains the secondary seed of Bacillus foecalis alkaligenes;
The denitrified pseudomonas (Pseudomonassp.denitrificans) that deposit number is ATCC13867 is cultivated in seed culture medium, obtains the first order seed of denitrified pseudomonas; The first order seed of denitrified pseudomonas is cultivated in seed culture medium, denitrified pseudomonas secondary seed;
(2) degradation solution preparation: for the ratio of 4-6:1, sulfamethoxazole is produced waste discharge by volume and mix with basic inorganic salt culture medium and be made into degradation solution;
(3) degradation solution is respectively charged in the first fermentation tank and the second fermentation tank; Being accessed by the secondary seed of Bacillus foecalis alkaligenes equipped with, in the first fermentation tank of degradation solution, being accessed by denitrified pseudomonas secondary seed equipped with in the second fermentation tank of degradation solution, ferment 12-16h;
(4) by the fermentation liquid in the first fermentation tank with 1-2L/h pump into the first hollow fiber film assembly filter the first ferment filtrate and filter after Bacillus foecalis alkaligenes, the first ferment filtrate continues to be input to the second fermentation tank; By the fermentation liquid in the second fermentation tank with 1-2L/h pump into the second hollow fiber film assembly filter the second ferment filtrate and filter after denitrified pseudomonas, the second ferment filtrate continues to be input to the first fermentation tank, is performed continuously over 2-3h; The first ferment filtrate inputs the first storage tank, and the second ferment filtrate inputs the second storage tank;
(5) with the first ferment filtrate in the first storage tank, the Bacillus foecalis alkaligenes after filtering is washed and returned in the first fermentation tank, with the second ferment filtrate in the second storage tank the denitrified pseudomonas after filtering washed and return in the second fermentation tank, ferment.
Basic inorganic salt culture medium is made up of in g/L following compositions: FeSO4��7H2O0.05g,MgSO4��7H2O1.20g,(NH4)2SO42.5g,Na2EDTA��2H2O0.09g,CaCl2��2H2O0.060g,Na2HPO422.0g,KH2PO420g, glucose 5g, sodium acetate 5g, natural pH.
The device of a kind of biodegradation sulfamethoxazole, including the first fermentation tank 1, the second fermentation tank 2, also include the first hollow fiber film assembly 3, the second hollow fiber film assembly 4, the first receiver 5, second receiver 6, the first pump 7, the second pump 8,3rd pump 9,4th pump 10, first throttle valve 11, second throttle 12,3rd choke valve 13, the 4th choke valve 14; One end of first fermentation liquid conveying pipe 15 is arranged on the middle and lower part in the first fermentation tank 1, and the top of the other end and the first hollow fiber film assembly 3 connects; First pump 7 is arranged on the first fermentation liquid conveying pipe; The top of the first hollow fiber film assembly 3 is connected with first throttle valve 11 and second throttle 12 respectively by pipeline, and first throttle valve 11 is connected by pipeline and the first receiver 5, and second throttle 12 is connected by pipeline and the second fermentation tank 2; First one end cleaning pipe 16 is arranged on the middle and lower part of the first receiver 5, and the top of the other end and the first hollow fiber film assembly 3 connects; Second pump 8 is arranged on the first cleaning pipe 16; The bottom of the first hollow fiber film assembly 3 is connected by the top of pipeline and the first fermentation tank 1; One end of second fermentation liquid conveying pipe 17 is arranged on the middle and lower part in the second fermentation tank 2, and the top of the other end and the second hollow fiber film assembly 4 connects;3rd pump 9 is arranged on the second fermentation liquid conveying pipe; The top of the second hollow fiber film assembly 4 is connected with the 3rd choke valve 13 and the 4th choke valve 14 respectively by pipeline, and the 3rd choke valve 13 is connected by pipeline and the second receiver 6, and the 4th choke valve 14 is connected by pipeline and the first fermentation tank 1; Second one end cleaning pipe 18 is arranged on the middle and lower part of the second receiver 6, and the top of the other end and the second hollow fiber film assembly 4 connects; 4th pump 10 is arranged on the second cleaning pipe 18; The bottom of the second hollow fiber film assembly 4 is connected by the top of pipeline and the second fermentation tank 2.
The invention have the advantages that
The present invention is in Alcaligenesfaecalis and Pseudomonassp.denitrificans mixed bacterium degradation SMX process, when biomass accumulation is to a certain extent (during fermentation 12-16h), start with the fermentation liquid in hollow-fibre membrane two fermentation tanks of exchange, two kinds of bacterial cells are not contacted, thus reducing because two kinds of somatic cells contact the suppression to Biomass brought thus causing that SMX degradation rate is not high. Hollow fibre membrane systems can promote the Biomass of two kinds of bacterium, it is suppressed that SMX synthesizes again.
Accompanying drawing explanation
Fig. 1 is the device schematic diagram of a kind of biodegradation sulfamethoxazole.
The fermentation process different for Fig. 2 impact on Fungal biodiversity.
Fermentation process different for Fig. 3 is on the SMX impact degraded.
Detailed description of the invention
Below in conjunction with concrete drawings and Examples, the present invention is further described:
The Bacillus foecalis alkaligenes (Alcaligenesfaecalis) that the present invention relates to, buys in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number: CGMCC1.767. Denitrified pseudomonas (Pseudomonassp.denitrificans), buys in American Type Culture Collecti, deposit number: ATCC13867.
Used by various embodiments of the present invention, seed culture based component is in g/L: beef extract 3, NaCl5, and peptone 10, pH is 7.0;
The device of a kind of biodegradation sulfamethoxazole, is shown in Fig. 1, including the first fermentation tank 1, second fermentation tank 2, also includes the first hollow fiber film assembly 3, the second hollow fiber film assembly 4, first receiver 5, the second receiver 6, the first pump 7, second pump 8, the 3rd pump 9, the 4th pump 10, first throttle valve 11, second throttle 12, the 3rd choke valve 13, the 4th choke valve 14; One end of first fermentation liquid conveying pipe 15 is arranged on the middle and lower part in the first fermentation tank 1, and the top of the other end and the first hollow fiber film assembly 3 connects; First pump 7 is arranged on the first fermentation liquid conveying pipe; The top of the first hollow fiber film assembly 3 is connected with first throttle valve 11 and second throttle 12 respectively by pipeline, and first throttle valve 11 is connected by pipeline and the first receiver 5, and second throttle 12 is connected by pipeline and the second fermentation tank 2; First one end cleaning pipe 16 is arranged on the middle and lower part of the first receiver 5, and the top of the other end and the first hollow fiber film assembly 3 connects; Second pump 8 is arranged on the first cleaning pipe 16; The bottom of the first hollow fiber film assembly 3 is connected by the top of pipeline and the first fermentation tank 1; One end of second fermentation liquid conveying pipe 17 is arranged on the middle and lower part in the second fermentation tank 2, and the top of the other end and the second hollow fiber film assembly 4 connects; 3rd pump 9 is arranged on the second fermentation liquid conveying pipe; The top of the second hollow fiber film assembly 4 is connected with the 3rd choke valve 13 and the 4th choke valve 14 respectively by pipeline, and the 3rd choke valve 13 is connected by pipeline and the second receiver 6, and the 4th choke valve 14 is connected by pipeline and the first fermentation tank 1;Second one end cleaning pipe 18 is arranged on the middle and lower part of the second receiver 6, and the top of the other end and the second hollow fiber film assembly 4 connects; 4th pump 10 is arranged on the second cleaning pipe 18; The bottom of the second hollow fiber film assembly 4 is connected by the top of pipeline and the second fermentation tank 2.
Embodiment 1
A kind of method of biodegradation sulfamethoxazole, comprises the steps:
(1) seed culture: the Bacillus foecalis alkaligenes that deposit number is CGMCCNo.1.767 (Alcaligenesfaecalis) that-80 DEG C store is accessed in 50mL seed culture medium, temperature 30 DEG C, when rotating speed 220rpm, cultivate 24h, cultivate, obtain the first order seed of Bacillus foecalis alkaligenes; By 1mL Bacillus foecalis alkaligenes first order seed access 100mL new seed culture medium in temperature 30 DEG C, when rotating speed 220rpm, cultivate 24h, obtain the secondary seed of Bacillus foecalis alkaligenes;
The denitrified pseudomonas that deposit number is ATCC13867 (Pseudomonassp.denitrificans) that-80 DEG C store is accessed in 50mL seed culture medium, temperature 30 DEG C, when rotating speed 220rpm, cultivate 24h, cultivate, obtain the first order seed of denitrified pseudomonas; The first order seed of 1mL denitrified pseudomonas is accessed in the new seed culture medium of 100mL temperature 30 DEG C, when rotating speed 220rpm, cultivate 24h, obtain the secondary seed of denitrified pseudomonas;
(2) degradation solution preparation: 2.4L sulfamethoxazole production waste discharge (sulfamethoxazole concentration is 65mg/L) is mixed with 0.6L basic inorganic salt culture medium and is made into degradation solution;
(3) for the device of a kind of biodegradation sulfamethoxazole of the present invention;
Degradation solution is respectively charged in the first fermentation tank 1 and the second fermentation tank 2; By the secondary seed of Bacillus foecalis alkaligenes by initial OD540Be 0.2 inoculum concentration access equipped with in the first fermentation tank 1 of 3L degradation solution, by denitrified pseudomonas secondary seed by initial OD540Be 0.2 inoculum concentration access equipped with in the second fermentation tank 2 of 3L degradation solution, be 0.5vvm in blowing air amount, speed of agitator is 300rpm, and temperature is the 12h that ferments respectively under 30 DEG C of conditions;
Hollow-fibre membrane sterilizing: hollow fiber film assembly 1g/L liquor natrii hypochloritis circulates stream half an hour, then with sterilized distilled water flushing 1 hour;
(4) opening the first pump 7, with 1L/h, the fermentation liquid in the first fermentation tank 1 is pumped into the first hollow fiber film assembly 3, and (hollow-fibre membrane is that 0.2 ��m and surface area are for 0.3m for aseptic aperture2) filter the first ferment filtrate and filter after Bacillus foecalis alkaligenes, close first throttle valve 11, open second throttle 12, the first ferment filtrate continues to be input in the second fermentation tank 2; The time opened with the first pump 7 is the same time open the 3rd pump 9, and with 1L/h, the fermentation liquid in the second fermentation tank 2 is pumped into the second hollow fiber film assembly 4, and (hollow-fibre membrane is that 0.2 ��m and surface area are for 0.3m for aseptic aperture2) filter the second ferment filtrate and filter after denitrified pseudomonas, close the 3rd choke valve 13, open the 4th choke valve 14, the second ferment filtrate continues to be input in the first fermentation tank 1; It is performed continuously over 2h;
Close second throttle 12, open first throttle valve 11, the first ferment filtrate is inputted the first storage tank, meanwhile, close the 4th choke valve 14, open the 3rd choke valve 13, the second ferment filtrate is inputted the second storage tank;
(5) the first pump the 7, the 3rd pump 9, first throttle valve 11, second throttle the 12, the 3rd choke valve the 13, the 4th choke valve 14 are closed, open the second pump the 8, the 4th pump 10, with the first ferment filtrate in the first storage tank 5, the Bacillus foecalis alkaligenes after filtering is washed and returned in the first fermentation tank 1, with the second ferment filtrate in the second storage tank 6, the denitrified pseudomonas after filtering is washed and returned in the second fermentation tank 2, fermentation.
Basic inorganic salt culture medium composition is in g/L: FeSO4��7H2O0.05g,MgSO4��7H2O1.20g,(NH4)2SO42.5g,Na2EDTA��2H2O0.09g,CaCl2��2H2O0.060g,Na2HPO422.0g,KH2PO420g, glucose 5g, sodium acetate 5g, natural pH, 121 DEG C of steam sterilization 20min;
The Biomass that each time period detects is shown in Fig. 2, and wherein AF (withHFM) is the Biomass of the first fermentation tank, and PD (withHFM) is the Biomass of the second fermentation tank; The SMX concentration that each time period detects is shown in that Fig. 3 wherein AF (withHFM) is the SMX concentration of the first fermentation tank, and PD (withHFM) is the SMX concentration of the second fermentation tank.
Detection adopts high performance liquid chromatography, and the chromatographic column used by high performance liquid chromatography is C18 post (250 �� 4.6mm, 5 ��m), and sample size is 10 �� L, and mobile phase is acetonitrile: 0.1% aqueous formic acid=40:60, and flow velocity is 1mL.min-1, detection wavelength is 265nm, and column temperature maintains 30 DEG C. The concentration of different time points detection SMX.
Embodiment 2
A kind of method of biodegradation sulfamethoxazole, comprises the steps:
(1) with (1) of embodiment 1;
(2) degradation solution preparation: 3L sulfamethoxazole production waste discharge (sulfamethoxazole concentration is 65mg/L) is mixed with 0.6L basic inorganic salt culture medium and is made into degradation solution;
(3) degradation solution is respectively charged in the first fermentation tank 1 and the second fermentation tank 2; By the secondary seed of Bacillus foecalis alkaligenes by initial OD540Be 0.2 inoculum concentration access equipped with in the first fermentation tank 1 of 3L degradation solution, by denitrified pseudomonas secondary seed by initial OD540Be 0.2 inoculum concentration access equipped with in the second fermentation tank 2 of 3L degradation solution, be 0.5vvm in blowing air amount, speed of agitator is 300rpm, and temperature is the 14h that ferments respectively under 30 DEG C of conditions;
(4) (hollow-fibre membrane is that 0.2 ��m and surface area are for 0.3m for aseptic aperture with 1.5L/h, the fermentation liquid in the first fermentation tank to be pumped into the first hollow fiber film assembly 32) filter the first ferment filtrate and filter after Bacillus foecalis alkaligenes, the first ferment filtrate continues to be input to the second fermentation tank 2; With 1.5L/h, the fermentation liquid in the second fermentation tank is pumped into the second hollow fiber film assembly 4, and (hollow-fibre membrane is that 0.2 ��m and surface area are for 0.3m for aseptic aperture2) filter the second ferment filtrate and filter after denitrified pseudomonas, the second ferment filtrate continues to be input to the first fermentation tank 1, is performed continuously over 3h; The first ferment filtrate inputs the first storage tank 5, and the second ferment filtrate inputs the second storage tank 6;
(5) with the first ferment filtrate in the first storage tank 5, the Bacillus foecalis alkaligenes after filtering is washed and returned in the first fermentation tank 1, with the second ferment filtrate in the second storage tank 6 denitrified pseudomonas after filtering washed and return in the second fermentation tank 2, ferment.
It is demonstrated experimentally that the present embodiment is when identical with the time point of embodiment 1, the Biomass of the first fermentation tank and the second fermentation tank and the concentration of respective SMX are similar to the result of embodiment 1.
Embodiment 3
A kind of method of biodegradation sulfamethoxazole, comprises the steps:
(1) with (1) of embodiment 1;
(2) degradation solution preparation: 3.6L sulfamethoxazole production waste discharge (sulfamethoxazole concentration is 65mg/L) is mixed with 0.6L basic inorganic salt culture medium and is made into degradation solution;
(3) degradation solution is respectively charged in the first fermentation tank 1 and the second fermentation tank 2; By the secondary seed of Bacillus foecalis alkaligenes by initial OD540Be 0.2 inoculum concentration access equipped with in the first fermentation tank 1 of 3L degradation solution, by denitrified pseudomonas secondary seed by initial OD540Be 0.2 inoculum concentration access equipped with in the second fermentation tank 2 of 3L degradation solution, be 0.5vvm in blowing air amount, speed of agitator is 300rpm, and temperature is the 16h that ferments respectively under 30 DEG C of conditions;
(4) (hollow-fibre membrane is that 0.2 ��m and surface area are for 0.3m for aseptic aperture with 2L/h, the fermentation liquid in the first fermentation tank to be pumped into the first hollow fiber film assembly 32) filter the first ferment filtrate and filter after Bacillus foecalis alkaligenes, the first ferment filtrate continues to be input to the second fermentation tank 2; With 2L/h, the fermentation liquid in the second fermentation tank is pumped into the second hollow fiber film assembly 4, and (hollow-fibre membrane is that 0.2 ��m and surface area are for 0.3m for aseptic aperture2) filter the second ferment filtrate and filter after denitrified pseudomonas, the second ferment filtrate continues to be input to the first fermentation tank 1, is performed continuously over 2.5h; The first ferment filtrate inputs the first storage tank 5, and the second ferment filtrate inputs the second storage tank 6;
(5) with the first ferment filtrate in the first storage tank 5, the Bacillus foecalis alkaligenes after filtering is washed and returned in the first fermentation tank 1, with the second ferment filtrate in the second storage tank 6 denitrified pseudomonas after filtering washed and return in the second fermentation tank 2, ferment.
It is demonstrated experimentally that the present embodiment is when identical with the time point of embodiment 1, the Biomass of the first fermentation tank and the second fermentation tank and the concentration of respective SMX are similar to the result of embodiment 1.
Comparative example 1
Bacillus foecalis alkaligenes and denitrified pseudomonas be single culture degraded SMX in two fermentation tanks, does not carry out fermentation liquid exchange. (1)��(2) are with embodiment 1 (1)��(2);
(3) by the secondary seed of Bacillus foecalis alkaligenes, denitrified pseudomonas secondary seed respectively by initial OD540Being the inoculum density of 0.2, access 5L equipped with in two fermentation tanks of 3L degradation solution, be 0.5vvm at ventilation, speed of agitator is 300rpm, and temperature is 30 DEG C, ferments. Biomass that each time period detects as in figure 2 it is shown, wherein AF be Bacillus foecalis alkaligenes degradation results; PD is denitrified pseudomonas degradation results; SMX concentration that each time period detects as it can be seen, wherein AF be Bacillus foecalis alkaligenes degradation results; PD is denitrified pseudomonas degradation results.
Comparative example 2
Bacillus foecalis alkaligenes and denitrified pseudomonas be Mixed culture degraded SMX in a fermentation tank, does not carry out fermentation liquid exchange. (1)��(2) are with embodiment 1 (1)��(2);
(3) by initial total OD540Being the inoculum density of 0.2, inoculative proportion is Bacillus foecalis alkaligenes and denitrified pseudomonas 1:1 inoculative proportion, accesses in the 5L fermentation tank equipped with 3L degradation solution, is 0.5vvm at ventilation, and speed of agitator is 300rpm, and temperature is 30 DEG C, ferments. In the Biomass that each time period detects such as Fig. 2 shown in AF&PD, AF&PD; Shown in AF&PD in SMX concentration such as Fig. 3 that each time period detects.
Claims (3)
1. a method for biodegradation sulfamethoxazole, its feature comprises the steps:
(1) seed culture: the Bacillus foecalis alkaligenes (Alcaligenesfaecalis) that deposit number is CGMCCNo.1.767 is cultivated in seed culture medium, obtains the first order seed of Bacillus foecalis alkaligenes; The first order seed of Bacillus foecalis alkaligenes is cultivated in seed culture medium, obtains the secondary seed of Bacillus foecalis alkaligenes;
The denitrified pseudomonas (Pseudomonassp.denitrificans) that deposit number is ATCC13867 is cultivated in seed culture medium, obtains the first order seed of denitrified pseudomonas; The first order seed of denitrified pseudomonas is cultivated in seed culture medium, denitrified pseudomonas secondary seed;
(2) degradation solution preparation: for the ratio of 4-6:1, sulfamethoxazole is produced waste discharge by volume and mix with basic inorganic salt culture medium and be made into degradation solution;
(3) degradation solution is respectively charged in the first fermentation tank and the second fermentation tank; Being accessed by the secondary seed of Bacillus foecalis alkaligenes equipped with, in the first fermentation tank of degradation solution, being accessed by denitrified pseudomonas secondary seed equipped with in the second fermentation tank of degradation solution, ferment 12-16h;
(4) by the fermentation liquid in the first fermentation tank with 1-2L/h pump into the first hollow fiber film assembly filter the first ferment filtrate and filter after Bacillus foecalis alkaligenes, the first ferment filtrate continues to be input to the second fermentation tank; By the fermentation liquid in the second fermentation tank with 1-2L/h pump into the second hollow fiber film assembly filter the second ferment filtrate and filter after denitrified pseudomonas, the second ferment filtrate continues to be input to the first fermentation tank, is performed continuously over 2-3h; The first ferment filtrate inputs the first storage tank, and the second ferment filtrate inputs the second storage tank;
(5) with the first ferment filtrate in the first storage tank, the Bacillus foecalis alkaligenes after filtering is washed and returned in the first fermentation tank, with the second ferment filtrate in the second storage tank the denitrified pseudomonas after filtering washed and return in the second fermentation tank, ferment.
2. the method for a kind of biodegradation sulfamethoxazole according to claim 1, is characterized in that described basic inorganic salt culture medium is made up of in g/L following compositions: FeSO4��7H2O0.05g,MgSO4��7H2O1.20g,(NH4)2SO42.5g,Na2EDTA��2H2O0.09g,CaCl2��2H2O0.060g,Na2HPO422.0g,KH2PO420g, glucose 5g, sodium acetate 5g, natural pH.
3. a device for biodegradation sulfamethoxazole, its feature includes the first fermentation tank 1, the second fermentation tank 2, it is characterized in that also including the first hollow fiber film assembly 3, the second hollow fiber film assembly 4, the first receiver 5, second receiver 6, the first pump 7, the second pump 8,3rd pump 9,4th pump 10, first throttle valve 11, second throttle 12,3rd choke valve 13, the 4th choke valve 14; One end of first fermentation liquid conveying pipe 15 is arranged on the middle and lower part in the first fermentation tank 1, and the top of the other end and the first hollow fiber film assembly 3 connects; First pump 7 is arranged on the first fermentation liquid conveying pipe; The top of the first hollow fiber film assembly 3 is connected with first throttle valve 11 and second throttle 12 respectively by pipeline, and first throttle valve 11 is connected by pipeline and the first receiver 5, and second throttle 12 is connected by pipeline and the second fermentation tank 2; First one end cleaning pipe 16 is arranged on the middle and lower part of the first receiver 5, and the top of the other end and the first hollow fiber film assembly 3 connects; Second pump 8 is arranged on the first cleaning pipe 16; The bottom of the first hollow fiber film assembly 3 is connected by the top of pipeline and the first fermentation tank 1; One end of second fermentation liquid conveying pipe 17 is arranged on the middle and lower part in the second fermentation tank 2, and the top of the other end and the second hollow fiber film assembly 4 connects; 3rd pump 9 is arranged on the second fermentation liquid conveying pipe; The top of the second hollow fiber film assembly 4 is connected with the 3rd choke valve 13 and the 4th choke valve 14 respectively by pipeline, and the 3rd choke valve 13 is connected by pipeline and the second receiver 6, and the 4th choke valve 14 is connected by pipeline and the first fermentation tank 1; Second one end cleaning pipe 18 is arranged on the middle and lower part of the second receiver 6, and the top of the other end and the second hollow fiber film assembly 4 connects; 4th pump 10 is arranged on the second cleaning pipe 18; The bottom of the second hollow fiber film assembly 4 is connected by the top of pipeline and the second fermentation tank 2.
Priority Applications (1)
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| CN201610107207.8A CN105645598B (en) | 2016-02-26 | 2016-02-26 | A kind of method and device of biodegradable sulfamethoxazole |
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| CN201610107207.8A CN105645598B (en) | 2016-02-26 | 2016-02-26 | A kind of method and device of biodegradable sulfamethoxazole |
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| CN105645598A true CN105645598A (en) | 2016-06-08 |
| CN105645598B CN105645598B (en) | 2018-02-13 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108251331A (en) * | 2017-12-29 | 2018-07-06 | 中国农业科学院农业环境与可持续发展研究所 | Water balsamine Bacillus alcaligenes and microbial bacterial agent and their applications in animal waste recycling |
| CZ307652B6 (en) * | 2017-12-21 | 2019-01-30 | ENVISAN-GEM, a.s. | A method for biodegrading sulfonamide-based pharmaceutically active substances in wastewater or surface water, a mixed whole cell catalyst |
| CN109825419A (en) * | 2019-03-27 | 2019-05-31 | 山东科技大学 | The tower cultivation reactor and its application method of wastewater treatment are used for based on list/Hybrid NC machine tool induction mineralising |
| CN114455718A (en) * | 2021-12-31 | 2022-05-10 | 江苏合普环保科技有限公司 | Method for treating sulfanilamide high-salt wastewater by using bioengineering bacteria |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4401762A (en) * | 1980-09-19 | 1983-08-30 | Societe Nationale Elf Aquitaine (Production) | Process of culturing microorganisms using a microemulsion |
| CN1908176A (en) * | 2006-08-21 | 2007-02-07 | 天津科技大学 | Method of realizing microorganism in situ separation and fermentation employing inorganic film |
| CN104694435A (en) * | 2015-03-13 | 2015-06-10 | 南京理工大学 | Shinella with triazole degradation function and application thereof |
-
2016
- 2016-02-26 CN CN201610107207.8A patent/CN105645598B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4401762A (en) * | 1980-09-19 | 1983-08-30 | Societe Nationale Elf Aquitaine (Production) | Process of culturing microorganisms using a microemulsion |
| US4460692A (en) * | 1980-09-19 | 1984-07-17 | Societe Nationale Elf Aquitaine (Production) | Microemulsion of nutrient substances |
| CN1908176A (en) * | 2006-08-21 | 2007-02-07 | 天津科技大学 | Method of realizing microorganism in situ separation and fermentation employing inorganic film |
| CN104694435A (en) * | 2015-03-13 | 2015-06-10 | 南京理工大学 | Shinella with triazole degradation function and application thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CZ307652B6 (en) * | 2017-12-21 | 2019-01-30 | ENVISAN-GEM, a.s. | A method for biodegrading sulfonamide-based pharmaceutically active substances in wastewater or surface water, a mixed whole cell catalyst |
| CN108251331A (en) * | 2017-12-29 | 2018-07-06 | 中国农业科学院农业环境与可持续发展研究所 | Water balsamine Bacillus alcaligenes and microbial bacterial agent and their applications in animal waste recycling |
| CN108251331B (en) * | 2017-12-29 | 2020-09-25 | 中国农业科学院农业环境与可持续发展研究所 | Impatiens balsamina alcaligenes and microbial agent and application thereof in recycling livestock and poultry breeding waste |
| CN109825419A (en) * | 2019-03-27 | 2019-05-31 | 山东科技大学 | The tower cultivation reactor and its application method of wastewater treatment are used for based on list/Hybrid NC machine tool induction mineralising |
| CN109825419B (en) * | 2019-03-27 | 2023-09-29 | 山东科技大学 | Tower-type culture reactor for wastewater treatment based on single/mixed bacteria culture induced mineralization and application method thereof |
| CN114455718A (en) * | 2021-12-31 | 2022-05-10 | 江苏合普环保科技有限公司 | Method for treating sulfanilamide high-salt wastewater by using bioengineering bacteria |
| CN114455718B (en) * | 2021-12-31 | 2022-12-09 | 江苏合普环保科技有限公司 | Method for treating sulfanilamide high-salt wastewater by using bioengineering bacteria |
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| CN105645598B (en) | 2018-02-13 |
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