CN101898821B - Bio-diatomite composite flocculant - Google Patents
Bio-diatomite composite flocculant Download PDFInfo
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- CN101898821B CN101898821B CN201010150879XA CN201010150879A CN101898821B CN 101898821 B CN101898821 B CN 101898821B CN 201010150879X A CN201010150879X A CN 201010150879XA CN 201010150879 A CN201010150879 A CN 201010150879A CN 101898821 B CN101898821 B CN 101898821B
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Abstract
The invention belongs to the water treatment field and discloses a bio-diatomite composite flocculant. The flocculant mainly contains refined diatomite and bioflocculant, wherein the weight of the bioflocculant is 10%-15% of that of refined diatomite; and the bioflocculant is an extracellular polymer prepared by selecting at least one of microbial strains with the preservation number CGMCC No. 3514, CGMCC No. 3515 and CGMCC No. 3516, fermenting, extracting, separating and purifying. The bio-diatomite composite flocculant is characterized by efficient flocculation, adsorption and filtration and environmental friend; and the flocculant can be directly added in the biochemical system of the existing sewage treatment system, thus industrial and engineering applications are easy to realize and the upgrading of the existing sewage treatment plants is also easy to realize.
Description
Technical field
The invention belongs to water treatment field, relate to a kind of biology-diatomite composite flocculant, be specifically related to a kind of biological flocculant and diatomite be combined the biology-diatomite composite flocculant that is used for advanced treatment of wastewater.
Background technology
Flocculation is to the important process in water security purification and the wastewater treatment, and flocculation agent directly influences flocculating effect.At present commercially available most of inorganic flocculating agents such as aluminium system and iron system have toxicity, influence ecological safety; Organic polymer coargulators such as polyacrylamide have neurotoxicity and teratogenesis, carcinogenic, mutagenic effect, make the application of traditional flocculant be very limited.
Advantages such as that microbial flocculant has is nontoxic, safe, biodegradable, non-secondary pollution in the water supply purification turbidity removal, are fallen in the wastewater treatment in the fields such as dirt, decolouring and are had the huge applications potentiality.And diatomite be ancient times unicellular lower plant diatom remains pile up after, what form through preliminary Diagn has a porous biological silicastone, have no side effect and have good biocompatibility, specific surface area that it is huge and suitable surperficial electronegativity possess characteristics such as flocculation, absorption, filtration simultaneously in wastewater treatment.
The composite flocculation agent that studies show that in recent years has more superiority than single flocculation agent, can realize learning from other's strong points to offset one's weaknesses, have complementary advantages, so just not only can improve flocculation efficiency, reduce flocculant dosage, reduce the mud generation, can also widen adaptability, and reusing sewage and sludge treatment and disposal are more prone to the sewage kind.Diatomite and organic floculant compound makes organic polymer coargulator and diatomite form organic assembling, can give full play to both synergy, processing efficiency is significantly improved." a kind of preparation method of organic polymer-diatomaceous soil composite coagulating agent " (200710068211.9) of the Zhan Shulin invention of Sanding Sci. ﹠ Tech. Co., Ltd., Zhejiang, after the diatomite ore raw material being carried out the lattice activation, compound with organic polymer coargulator, the cooperate optimization effect of performance polymeric flocculant and modification infusorial earth mineral coagulant aid, thus prepare the organic polymer-diatomaceous soil composite coagulating agent that textile dyeing and finishing, paper industry sewage is had remarkable purification and decoloration effect.Yet, these composite flocculation agents are not that to contain aluminium system/iron be that inorganic flocculating agent contains organic polymer coargulator exactly, have toxicity and environmental pollution, or cost an arm and a leg, in use tend to the operation of generate subsequent metallization processes is brought certain influence, also can cause secondary pollution, human health and ecotope are brought disadvantageous effect, all the proper environmentally friendly composite flocculation agent of right and wrong.
Summary of the invention
The objective of the invention is at existing sewage treatment process processing efficiency lowly, effluent quality has much room for improvement after the advanced treatment, and adding of flocculation agent easily causes secondary pollution problems, and a kind of efficient, environmentally friendly biology-diatomite composite flocculant is provided.
Purpose of the present invention can be achieved through the following technical solutions:
A kind of biology-diatomite composite flocculant, this flocculation agent mainly contains diatomite and biological flocculant, and the biofloculation agent content is the 10wt%～150wt% of diatomite.
Wherein, diatom shell content reaches more than the 85wt% in the described diatomite.
Described diatomite preferably has the diatomite of following quality and specification: diatom shell content reaches more than the 85wt%, and silicon shell integrity, air permeability are better, and color is a white, H
2O content≤1%, tight bulk density is 0.3～0.4g/cm
3, specific surface area reaches 50～60m
2/ g contains SiO
2〉=85wt%, Al
2O
3≤ 5%, Fe
2O
3≤ 1.8%, rate of permeation: 1.5-3.5, wet density≤0.35g/cm
3, 200～300 mesh sieves.
Described biological flocculant by be selected from preserving number be among CGMCC No.3514, CGMCC No.3515, the CGMCC No.3516 at least a flocculating microbial bacterial strain by fermentation, the extracellular polymeric that obtains of extraction, separation, purifying; Be preferably selected from preserving number and be among CGMCC No.3514, CGMCC No.3515, the CGMCC No.3516 any two kinds of microorganism strains by fermentation, the extracellular polymeric that obtains of extraction, separation, purifying; Especially preferably by preserving number be CGMCCNo.3514, CGMCC No.3515 and CGMCC No.3516 three kinds of microorganism strains by fermentation, the extracellular polymeric that obtains of extraction, separation, purifying.
The preparation method of biology-diatomite composite flocculant of the present invention:
The first step: the microorganism strains fermentation culture that will produce flocculation respectively is to the stable growth phase, the centrifugal thalline of removing of fermented liquid, and concentrated supernatant adds the CaCl be equivalent to supernatant liquor weight 0.05-0.15% in supernatant liquor
2Dehydrated alcohol with 2～3 times of supernatant liquor volumes leaves standstill, and the centrifugal 15min of 12000g abandons supernatant, and it is standby to get throw out 1; With the sodium chloride solution dissolving of throw out 1 usefulness 0.5-3.0%, pH5-6, with 12% cetyl trimethylammonium bromide solution absorbs of 0.3-1 times of sodium chloride solution volume, it is standby to get throw out 2 through centrifugation again; 6-18h is stirred in the sodium-chlor dissolving of throw out 2 usefulness 5-15%, add the dehydrated alcohol of 2.5-3.5 times of NaCl volume, stir, leave standstill, the centrifugal throw out 3 that gets throw out 3 usefulness dehydrated alcohols or ether dilution, gets the biological flocculant product with its vacuum-drying 5-6h then.
Second step: add accounting for the biological flocculant product that diatomite quality 10wt%～150wt% is made by the first step in the diatomite,, be mixed with biology-diatomite composite flocculant through behind the uniform mixing.
Wherein, described diatomite reaches the above diatomite of 85wt% for diatom shell content; This diatomite can select diatom original soil by existing beneficiation method (pickling, calcining or physical concentration etc.) and wash acquisition, also can buy by the commercial channel.
Described diatomite preferably has the diatomite of following quality and specification: diatom shell content reaches more than the 85wt%, and silicon shell integrity, air permeability are better, and color is a white, H
2O content≤1%, tight bulk density is 0.3～0.4g/cm
3, specific surface area reaches 50～60m
2/ g contains SiO
2: 〉=85wt%, Al
2O
3:≤5%, Fe
2O
3:≤1.8%, rate of permeation: 1.5-3.5, wet density≤0.35g/cm
3, 200～300 mesh sieves.
Described flocculating microbial bacterial strain is selected from least a in the microorganism that preserving number is CGMCC No.3514, CGMCC No.3515, CGMCCNo.3516; Be preferably selected from preserving number and be any two kinds of microorganisms among CGMCC No.3514, CGMCC No.3515, the CGMCC No.3516, being preferably selected from preserving number especially is three kinds of microorganisms of CGMCC No.3514, CGMCC No.3515 and CGMCC No.3516.
This biology-diatomite composite flocculant requires to prepare in the week before use according to different quality, and when preserving X-ray thoroughly, prevent that simultaneously it from shining for a long time in the sun.Biology-the diatomite composite flocculant for preparing is put in the sewage by the dosage of 10～100ppm and is handled.
The application of described biology-diatomite composite flocculant in water treatment, preferably application in high density town domestic sewage upgrading or the application in the high chroma decolorizing printing and dyeing waste water.
A kind of oligotrophy Zymomonas mobilis (Stenotrophomonas sp.) 46C as water treatment flocculant is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCC No.3514.
A kind of Providence (Providencia sp.) 12A as water treatment flocculant is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCC No.3515.
A kind of fragrance bacterium (Myroides sp.) 122B as water treatment flocculant is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCC No.3516.
The feature of this three strains microorganism strains is as follows respectively:
1. Providence (Providencia sp.) 12A
This bacterium Gram-reaction feminine gender, shaft-like, 2-5 * 0.5-0.8 μ m, bacterium colony are circular, diameter 1.8-2.2mm, light, thickness, projection, opaque, the edge is smooth, does not have gemma, chemoheterotrophy, necessary for growth oxygen, light requirement photograph.Nutrition there is not particular requirement, 10 ℃～43 ℃ of growth temperatures, optimum temperuture is 37 ℃.Can utilize citric acid, the fermentation of D-seminose, Oxybenzene L-Ala deamination and tryptophane deamination, the catalase positive can be produced acid, the indole reaction positive from seminose.Oxydase, arginine decarboxylase, lipase, ornithine decarboxylase feminine gender can not be utilized propanedioic acid, and the pure and mild L-arabinose of D-sorb (sugar) produces acid, can not liquefy gelatin, can not produce H
2S.
2. oligotrophy Zymomonas mobilis (Stenotrophomonas sp.) 46C
Germ oligotrophy unit cell is that obligate aerobic non-fermented type Gram-negative is extremely given birth to many flagellums bacillus, and strong ammonia flavor is arranged on blood agar, is β haemolysis; Show sallow pigment or non-pigment on nutrient agar medium, bacterium colony is tip-like, diameter 0.5mm～1mm, central protrusion.Reduction nitrate is nitrite, oxidase negative, strong solution fat, the DNase positive, gelatin hydrolysate and Vitamin C2, the lysine decarboxylase positive.In the oxidative fermentation test, produce slow acid, decompose maltose, produce acid obviously.Can utilize materials such as glucose, seminose, sucrose, gill fungus sugar, maltose, cellobiose, lactose, salicin, acetate and propionic salt.
3. fragrance bacterium (Myroides sp.) 122B
Gram-negative, strict aerobic growth, shaft-like 0.5-0.7 * 1-2 μ m, atrichia does not have cell to slide and group's trip ability, the bacterium colony yellow, fruity is arranged, can be in 18 ℃～40 ℃ growths, 37 ℃ of optimum growth temperatures, be lower than 5 ℃ and be higher than 42 ℃ not long, oxydase, catalase, gel lytic enzyme reacting positive, indole reaction feminine gender, can reduce nitrite and can not reduce nitrate, bacterial strain can not the hydrolysis arginine, and no betagalactosidase activity can not the hydrolysis polychrom.Can utilize the L-aspartic acid, L-L-glutamic acid and the growth of L-proline(Pro), energy oxidation Tween 40, Tween 80, methylacetone hydrochlorate, methylsuccinic acid, acetate, the Alpha-hydroxy pyruvic acid can not oxidation dextrin and glycogen glue.
3 plant heights that are separated to are imitated product flocculating microbial bacterial strain ferment, extract its extracellular polymeric, that extracting method adopts is Kellems ﹠amp; The method of Lion (Kellems B.L.and Lion L.W., Effect of bacterial exopolymer onlead (II) adsorption by γ Al
2O
3In seawater.Est.Coastal.Shelf Sci.1989 28:443-457), obtains the biological flocculant product after the vacuum-drying.UV scanning finds all have than tangible absorption peak at three strain bacterium 260nm places, 280nm does not have absorption peak, no colour-change is found in triketohydrindene hydrate colour developing experiment, the Molish reaction finds that separation surface has color, purple ring clearly appears, proof has a large amount of polysaccharide to produce, finally record Providencia sp.12A by phenolsulfuric acid method mensuration polysaccharide content and contain 90.24% polysaccharide, Stenotrophomonas sp.46C contains 94.13% polysaccharide, and Myroides sp.122B contains 89.33% polysaccharide.The content that ultraviolet absorption method is measured nucleic acid shows that Providencia sp.12A contains 3.17% nucleic acid, and Stenotrophomonas sp.46C contains 2.05% nucleic acid, and Myroides sp.122B contains 7.21% nucleic acid.
Beneficial effect of the present invention:
Three strain flocculating microbial flocculating effect excellences provided by the invention, the outer superpolymer of its born of the same parents is multiple biomacromolecule, can be by " adsorption bridging ", electrically neutralize, net is caught and volume is swept and effect such as chemical reaction makes aggegation, precipitations such as solid suspended particle in the water body, somatic cells and colloidal particle.The diatom shell enrichment that diatomite is made up of nonconducting amorphous build silicon-dioxide forms, particle diameter is minimum, the diatomite internal void is many, series connection communicates between hole, have huge specific surface and suitable surperficial electronegativity, can be used as a kind of good porous biological carrier, in wastewater treatment, possess flocculation, absorption simultaneously, filter three aspect effect, processing efficiency is compared traditional inorganic flocculating agent height.Biology-diatomite composite flocculant provided by the invention organically combines both, realization is learnt from other's strong points to offset one's weaknesses, have complementary advantages, not only can improve flocculation efficiency, reduce flocculant dosage, reduce the mud generation, improve other indexs of effluent quality, can also widen adaptability, and reusing sewage and sludge treatment and disposal are more prone to the sewage kind.
This biology-diatomite composite flocculant not only has characteristics such as efficient flocculating, absorption, filtration and environment friendly; And can directly be added in the biochemical system of existing Sewage treatment systems, be easier to realize the application of industrialization and through engineering approaches, can be widely used in high density town domestic sewage advanced treatment, high chroma decolorizing printing and dyeing waste water etc., traditional flocculant in the Drinking Water in China purification is substituted, the water pollution control advanced treatment, existing sewage work upgrading is realized purifying and the wastewater efficient processing to water security, guarantees that China's water resources safety and Environmental Health etc. have positive effect.
Our company---Jiangsu Jiaqing Water Treatment Co., Ltd.---had applied for once before that the patent No. was 200710196727.1 patent " a kind of biology diatom water treatment agent and preparation method thereof ", the present invention has the following advantages by comparison: what 1. the former adopted is conventional engineering of water treatment bacterium, what the present invention adopted is the extracellular polymeric that is extracted by the specified microorganisms bacterial classification, active better, specific aim is stronger, mainly be at high density town domestic sewage advanced treatment, high chroma decolorizing printing and dyeing waste water etc., effect is better, and when being applied in sewage disposal, adaptability is strong, in being added to water body, microbial flocculant can rapid reaction, and system can start fast.2. the present invention is applicable in the upgrading of existing sewage treatment facility, directly is added in the biochemical system, compares with the former, and special medicament throwing device need be set.3. the present invention is applied in the sewage disposal, and the sludge quantity of generation is few, for newly-built Sewage treatment systems, can not establish special settling tank, has saved one and has built and working cost.
Culture presevation information
1, oligotrophy Zymomonas mobilis (Stenotrophomonas sp.) 46C, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is CGMCC No.3514, and preservation day is on December 17th, 2009, and the address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
2, Providence (Providencia sp.) 12A, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is CGMCC No.3515, and preservation day is on December 17th, 2009, and the address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
3, fragrance bacterium (Myroides sp.) 122B is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCC No.3516, and preservation day is on December 17th, 2009, and the address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
Embodiment
The screening that embodiment 1 produces the flocculating microbial bacterial strain
Get the Nanjing lock Jin Cun Aerobic Pond active sludge sample 10g of sewage work, at basic salt culture medium (1.5g K
2HPO
4, 0.5g KH
2PO
4, 1.0g NH
4NO
3, 0.2g MgSO
4, 1.0g NaCl/L water, pH7.0) in 30 ℃ cultivate 24h, nutrient solution with the sterilization filter paper filtering, behind the aseptic water washing filter paper is washed the flco on the filter paper, be added in the basic salt culture medium of sterilization, carrying out pure bacterium after example enrichment is cultivated 4 times separates.Pregnant solution is coated Luria-Bertani (LB) and is supported base, cultivate 48h for 30 ℃, select smooth surface and have single bacterium colony of viscosity, measure its flocculation activity, choose conduct and sieve bacterial classification again, obtain the best bacterium for producing flocculant Providencia sp.12A (CGMCC No.3515) of three strain flocculating effects, Stenotrophomonas sp.46C (CGMCC No.3514) and Myroides sp.122B (CGMCC No.3516) through repeated screening and purifying with higher flocculation activity.
Embodiment 2
Produce the fermentation culture of wadding microorganism: under aseptic condition, with 3 strain bacterial strains (CGMCC No.3515, CGMCC No.3514 and CGMCC No.3516) respectively after slant activation, inoculate the LB culture media shaking vase respectively, shaking speed 200r/min, incubation time 50h is to logarithmic growth mid-term, be inoculated in first class seed pot by 5% inoculum size, the secondary seed jar is amplification culture step by step, makes the bacterium number and reaches OD value 1.0-1.2 * 10
9The fermented liq of/ml.Fermention medium is a glucose 1%, sulfuric acid amine 0.15%, and dipotassium hydrogen phosphate 0.05%, potassium primary phosphate 0.05%, sal epsom 0.002%, NaCl 0.02%, calcium chloride 0.01%, all the other are tap water, pH7.0,28～30 ℃ of fermentations, tank pressure 0.5kg/cm
2, air flow is with fermentating liquid volume m
3/ volume of air m
3Min meter 1: 0.8, mixing speed is 150r/min, pH value about 6.5, aerobic fermentation 65h.
Embodiment 3
With cultivating respectively among the embodiment 2 behind the 2d to the centrifugal 30min of fermented liquid 5000rpm of stable growth phase, remove thalline (precipitation), concentrate (conventional ultrafiltration, decompression) supernatant liquor, in supernatant liquor, add the CaCl that is equivalent to supernatant liquor weight 0.1%
2With the dehydrated alcohol of 2.5 times of supernatant liquor volumes, room temperature leaves standstill 40min, uses the centrifugal 15min of whizzer 12000g again, abandons supernatant, and it is standby to get throw out 1; With the dissolving of the sodium chloride solution of throw out 1 usefulness 3.0%, pH5 (sodium chloride solution can just dissolution precipitation thing 1 get final product), with 12% cetyl trimethylammonium bromide solution absorbs of 1 times of sodium chloride solution volume, it be standby to get throw out 2 through centrifugation again; 12h is stirred in the sodium-chlor dissolving of throw out 2 usefulness 10%, add the dehydrated alcohol of 3 times of NaCl liquor capacities, stir, leave standstill, the centrifugal throw out 3 that gets of 12000g the dilution of throw out 3 usefulness dehydrated alcohols, gets the biological flocculant product with its vacuum-drying 6h then.
Embodiment 4～9, Comparative Examples 3, embodiment 10～16 used biological flocculant products prepare by present embodiment.
Embodiment 4
At diatom shell content is to add the biological flocculant product (three kinds of biological flocculant weight ratios were followed successively by 1: 1: 1) that is prepared by Providencia sp.12A (CGMCC No.3515), Stenotrophomonas sp.46C (CGMCC No.3514) and three kinds of bacterial strains of Myroides sp.122B (CGMCC No.3516) that is equivalent to diatomite weight 20wt% in the diatomite of 85wt%, after evenly stirring, be mixed with biology-diatomite composite flocculant 1.
Embodiment 5
At diatom shell content is to add the biological flocculant product (three kinds of biological flocculant weight ratios were followed successively by 1: 1: 2) that is prepared by Providencia sp.12A (CGMCC No.3515), Stenotrophomonas sp.46C (CGMCC No.3514) and three kinds of bacterial strains of Myroides sp.122B (CGMCC No.3516) that is equivalent to diatomite weight 50wt% in the diatomite of 85wt%, after evenly stirring, be mixed with biology-diatomite composite flocculant 2.
Embodiment 6
At diatom shell content is to add the biological flocculant product (two kinds of biological flocculant weight ratios were followed successively by 2: 1) that is prepared by Providencia sp.12A (CGMCC No.3515) and two kinds of bacterial strains of Myroides sp.122B (CGMCC No.3516) that is equivalent to diatomite weight 80wt% in the diatomite of 85wt%, after evenly stirring, be mixed with biology-diatomite composite flocculant 3.
Embodiment 7
At diatom shell content is to add the biological flocculant product by Providencia sp.12A (CGMCC No.3515) preparation that is equivalent to diatomite weight 30wt% in the diatomite of 85wt%, after evenly stirring, be mixed with biology-diatomite composite flocculant 4.
Embodiment 8
At diatom shell content is to add the biological flocculant product by the preparation of Myroides sp.122B (CGMCC No.3516) bacterial strain that is equivalent to diatomite weight 50wt% in the diatomite of 85wt%, after evenly stirring, be mixed with biology-diatomite composite flocculant 5.
Embodiment 9
At diatom shell content is to add the biological flocculant product by the preparation of Stenotrophomonas sp.46C (CGMCC No.3514) bacterial strain that is equivalent to diatomite weight 80wt% in the diatomite of 85wt%, after evenly stirring, be mixed with biology-diatomite composite flocculant 6.
Comparative Examples 1
Conventional gac water conditioner (index)
Comparative Examples 2
(its molecule is [AL to conventional commercially available flocculant of polymeric aluminium chloride
2(OH) nCL
6-n] m, n≤5m≤10, aluminum oxide (AL
2O
3) content 〉=32.0%)
Comparative Examples 3
Biological flocculant product (three kinds of biological flocculant weight ratios are 1: 1: 1) by Providencia sp.12A (CGMCC No.3515), Stenotrophomonas sp.46C (CGMCC No.3514) and three kinds of bacterial strain preparations of Myroides sp.122B (CGMCC No.3516), after evenly stirring, be mixed with the compound bio flocculation agent.
Comparative Examples 4
The patent No. is 200710196727.1 " biological diatomite water conditioner ":
After diatom content is to add 50% flocculation agent aluminium chlorohydroxide in the diatomite more than 70%, add 5% particle diameter again at 200～400 purpose gacs, after even stirring, be mixed with the sewage-treating agent first product; The interpolation weight ratio is 9 ‰ engineering of water treatment bacterium super-CM biological bacteria in first product, after it is evenly stirred, is mixed with the required biology diatom water treatment agent of sewage disposal.
Application example
On the one hand, investigate the flocculating effect of embodiment, Comparative Examples.
The mensuration of flocculating effect: with adding 1000mL distilled water, 4g kaolin, the CaCl of 0.2g in the 1000mL beaker
2, the kaolin suspension liquid of preparation 4g/L.In graduated cylinder, measure a certain amount of kaolin suspension liquid and place beaker, add flocculation agent, regulate pH to 7.0, be placed on six agitators and stir 1min fast with 200rpm, 80rpm stirs 2min at a slow speed, leaves standstill 3min, draws the liquid layer of 50% degree of depth with the transfer pipet pipe and measures absorbancy OD550 in 722 type spectrophotometer 550nm, replace fermented liquid to compare with distilled water simultaneously, determine the flocculating degree of flocculation agent.Flocculating rate=(A-B)/A * 100; A is the absorbancy at contrast supernatant liquor 550nm place; B is the absorbancy at sample supernatant liquor 550nm place, does reference with distilled water.
Concrete outcome is as shown in table 1.
The comparison of table 1 embodiment, Comparative Examples flocculating effect
| Embodiment 4 | Embodiment 5 | Embodiment 6 | Embodiment 7 | Embodiment 8 | Embodiment 9 | |
| Flocculating effect | 96.6％ | 98.6％ | 94.3％ | 86.6％ | 85.7％ | 90.2％ |
| Comparative Examples 1 | Comparative Examples 2 | Comparative Examples 3 | Comparative Examples 4 |
| Flocculating effect | - | 78.5％ | 74.8％ | 79.3 |
On the other hand, the product with embodiment 4-9 and Comparative Examples 1 preparation adds respectively to the biochemical unit of conventional A/O biochemical process; The product of Comparative Examples 2, Comparative Examples 3 and Comparative Examples 4 preparations is added respectively to the biochemical follow-up mud-water separation of A/O technology unit as advanced treatment agent.Contrast the turbidity removal in sanitary sewage disposal of each embodiment, Comparative Examples, fall dirt and dephosphorization denitrogenation ability, concrete outcome is as shown in table 2.
Table 2 embodiment, Comparative Examples add the comparison of back system to the clearance of indexs such as SS in the sanitary sewage
| Embodiment 4 | Embodiment 5 | Embodiment 6 | Embodiment 7 | Embodiment 8 | Embodiment 9 | |
| SS | 97.55％ | 97.80％ | 97.46％ | 97.52％ | 97.34％ | 97.60％ |
| CODcr | 86.3％ | 86.5％ | 85.8％ | 85.2％ | 85.8％ | 86.2％ |
| Ammonia nitrogen | 98.85wt％ | 98.64％ | 98.36％ | 98.35％ | 98.19％ | 98.55％ |
| T-N | 78.84％ | 78.41％ | 77.97％ | 78.60％ | 78.56％ | 78.14％ |
| T-P | 90.27％ | 90.14％ | 90.08％ | 90.22％ | 90.12％ | 90.05％ |
| Comparative Examples 1 | Comparative Examples 2 | Comparative Examples 3 | Comparative Examples 4 | |||
| SS | 88.52％ | 93.45& | 90.88％ | 92.67％ | ||
| CODcr | 84.0％ | 80.7％ | 78.5％ | 81.3％ | ||
| Ammonia nitrogen | 95.62％ | 86.79％ | 88.34％ | 90.12％ | ||
| T-N | 76.03％ | 67.45％ | 67.62％ | 70.91％ | ||
| T-P | 78.37％ | 87.03％ | 80.45％ | 83.48％ |
Embodiment 4-9 can directly add in biochemical unit owing to do not have toxic, utilize diatomite wherein can be used as microbial flocculant carrier, strengthen biochemical action and himself dephosphorization function, improve system's dephosphorization denitrogenation ability.In addition, biology-diatomite composite flocculant also improves biochemical system active sludge sedimentation capacity, improves terminal mud-water separation efficient, reduces the water outlet concentration of suspension.
Embodiment 10
At diatom shell content is to add the biological flocculant product (three kinds of biological flocculant weight ratios were followed successively by 1: 1: 1) that is prepared by Providencia sp.12A (CGMCC No.3515), Stenotrophomonas sp.46C (CGMCC No.3514) and three kinds of bacterial strains of Myroides sp.122B (CGMCC No.3516) that is equivalent to diatomite weight 5wt% in the diatomite of 85wt%, after evenly stirring, be mixed with biology-diatomite composite flocculant, flocculating effect is 79.3%.
Embodiment 11
At diatom shell content is to add the biological flocculant product (three kinds of biological flocculant weight ratios were followed successively by 1: 1: 1) that is prepared by Providencia sp.12A (CGMCC No.3515), Stenotrophomonas sp.46C (CGMCC No.3514) and three kinds of bacterial strains of Myroides sp.122B (CGMCC No.3516) that is equivalent to diatomite weight 10wt% in the diatomite of 85wt%, after evenly stirring, be mixed with biology-diatomite composite flocculant, flocculating effect is 87%.
Embodiment 12
At diatom shell content is to add the biological flocculant product (three kinds of biological flocculant weight ratios were followed successively by 1: 1: 1) that is prepared by Providencia sp.12A (CGMCC No.3515), Stenotrophomonas sp.46C (CGMCC No.3514) and three kinds of bacterial strains of Myroides sp.122B (CGMCC No.3516) that is equivalent to diatomite weight 70wt% in the diatomite of 85wt%, after evenly stirring, be mixed with biology-diatomite composite flocculant, flocculating effect is 98.8%.。
Embodiment 13
At diatom shell content is to add the biological flocculant product (three kinds of biological flocculant weight ratios were followed successively by 1: 1: 1) that is prepared by Providencia sp.12A (CGMCC No.3515), Stenotrophomonas sp.46C (CGMCC No.3514) and three kinds of bacterial strains of Myroides sp.122B (CGMCC No.3516) that is equivalent to diatomite weight 100wt% in the diatomite of 85wt%, after evenly stirring, be mixed with biology-diatomite composite flocculant, flocculating effect is 99.0%.
Embodiment 14
At diatom shell content is to add the biological flocculant product (three kinds of biological flocculant weight ratios were followed successively by 1: 1: 1) that is prepared by Providencia sp.12A (CGMCC No.3515), Stenotrophomonas sp.46C (CGMCC No.3514) and three kinds of bacterial strains of Myroides sp.122B (CGMCC No.3516) that is equivalent to diatomite weight 120wt% in the diatomite of 85wt%, after evenly stirring, be mixed with biology-diatomite composite flocculant, flocculating effect is 99.0%.
Embodiment 15
At diatom shell content is to add the biological flocculant product (three kinds of biological flocculant weight ratios were followed successively by 1: 1: 1) that is prepared by Providencia sp.12A (CGMCC No.3515), Stenotrophomonas sp.46C (CGMCC No.3514) and three kinds of bacterial strains of Myroides sp.122B (CGMCC No.3516) that is equivalent to diatomite weight 150wt% in the diatomite of 85wt%, after evenly stirring, be mixed with biology-diatomite composite flocculant, flocculating effect is 99.1%.
Embodiment 16
At diatom shell content is to add the biological flocculant product (three kinds of biological flocculant weight ratios were followed successively by 1: 1: 1) that is prepared by Providencia sp.12A (CGMCC No.3515), Stenotrophomonas sp.46C (CGMCC No.3514) and three kinds of bacterial strains of Myroides sp.122B (CGMCC No.3516) that is equivalent to diatomite weight 170wt% in the diatomite of 85wt%, after evenly stirring, be mixed with biology-diatomite composite flocculant, flocculating effect is 81.4%.
The operation steps that the present invention does not describe in detail is the routine techniques in this area.
Claims (14)
1. biology-diatomite composite flocculant, it is characterized in that this flocculation agent mainly contains diatomite and biological flocculant, described biological flocculant be by be selected from preserving number be among CGMCC No.3514, CGMCC No.3515, the CGMCC No.3516 at least a microorganism strains by fermentation, the extracellular polymeric that obtains of extraction, separation, purifying, its content is the 10wt%～150wt% of diatomite.
2. biology-diatomite composite flocculant according to claim 1 is characterized in that described diatomite reaches the above diatomite of 85wt% for diatom shell content.
3. biology-diatomite composite flocculant according to claim 1, it is characterized in that described biological flocculant by be selected from preserving number be among CGMCC No.3514, CGMCC No.3515, the CGMCC No.3516 any two kinds of microorganism strains by fermentation, the extracellular polymeric that obtains of extraction, separation, purifying.
4. biology-diatomite composite flocculant according to claim 1, it is characterized in that described biological flocculant by preserving number be CGMCC No.3514, CGMCC No.3515 and CGMCC No.3516 three kinds of microorganism strains by fermentation, the extracellular polymeric that obtains of extraction, separation, purifying.
5. the preparation method of the described biology-diatomite composite flocculant of claim 1 is characterized in that:
The first step: the microorganism strains fermentation culture that will produce flocculation respectively is to the stable growth phase, the centrifugal thalline of removing of fermented liquid, and concentrated supernatant adds the CaCl be equivalent to supernatant liquor weight 0.05-0.15% in supernatant liquor
2Dehydrated alcohol with 2～3 times of supernatant liquor volumes leaves standstill, and it is standby that centrifugation gets throw out 1; With the sodium chloride solution dissolving of throw out 1 usefulness 0.5-3.0%, pH5-6, with 12% cetyl trimethylammonium bromide solution absorbs of 0.3-1 times of sodium chloride solution volume, it is standby to get throw out 2 through centrifugation again; 6-18h is stirred in the sodium-chlor dissolving of throw out 2 usefulness 5-15%, add the dehydrated alcohol of 2.5-3.5 times of NaCl volume, stir, the centrifugal throw out 3 that gets, throw out 3 usefulness dehydrated alcohols or ether dilution, then its vacuum-drying 5-6h is got the biological flocculant product;
Second step: add accounting for the biological flocculant product that diatomite 10wt%～150wt% is made by the first step in the diatomite,, be mixed with biology-diatomite composite flocculant through behind the uniform mixing.
6. preparation method according to claim 5 is characterized in that described diatomite reaches the above diatomite of 85wt% for diatom shell content.
7. preparation method according to claim 5, the microorganism strains that it is characterized in that the flocculation of described product is selected from least a in the microorganism strains that preserving number is CGMCC No.3514, CGMCC No.3515, CGMCC No.3516.
8. preparation method according to claim 7, it is any two kinds of microorganism strains among CGMCC No.3514, CGMCC No.3515, the CGMCC No.3516 that the microorganism strains that it is characterized in that the flocculation of described product is selected from preserving number.
9. preparation method according to claim 7 is characterized in that the microorganism strains of described product flocculation is CGMCC No.3514, CGMCC No.3515 and CGMCC No.3516.
10. the application of the described biology-diatomite composite flocculant of claim 1 in water treatment.
11. application according to claim 10 is characterized in that the application of described biology-diatomite composite flocculant in high density town domestic sewage upgrading.
12. oligotrophy Zymomonas mobilis (Stenotrophomonas sp.) 46C who is used to prepare the described biology-diatomite composite flocculant of claim 1 is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is CGMCC No.3514.
13. Providence (Providencia sp.) 12A who is used to prepare the described biology-diatomite composite flocculant of claim 1 is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is CGMCC No.3515.
14. fragrance bacterium (Myroidessp.) 122B who is used to prepare the described biology-diatomite composite flocculant of claim 1 is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is CGMCC No.3516.
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| CN102616909B (en) * | 2012-03-28 | 2013-03-27 | 江苏省嘉庆水务发展有限公司 | Advanced treatment method for diatomite for reclaimed-water recycling |
| CN108585220A (en) * | 2018-05-15 | 2018-09-28 | 合肥谦尧建筑装饰工程有限公司 | A kind of preparation method of domestic sewage in rural areas efficient process agent |
| CN109626596A (en) * | 2019-01-16 | 2019-04-16 | 合肥市东方美捷分子材料技术有限公司 | A kind of preparation method and applications of effective microorganisms |
| CN113087034A (en) * | 2021-03-31 | 2021-07-09 | 南京金湖环保科技有限公司 | Coated purification deodorant for sewage treatment and preparation process thereof |
| CN113105541B (en) * | 2021-04-23 | 2022-03-11 | 浙江崇山生物制品有限公司 | Method for purifying collagen |
| CN115650448B (en) * | 2022-02-15 | 2023-05-16 | 扬州工业职业技术学院 | Composite flocculant based on modified diatomite and application thereof |
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| CN100427595C (en) * | 2007-08-01 | 2008-10-22 | 江苏省嘉庆水务发展有限公司 | Biology diatom water treatment agent and method of producing the same |
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