CN112226390A - Compound functional microbial inoculum for composting fermentation and kitchen waste composting method - Google Patents
Compound functional microbial inoculum for composting fermentation and kitchen waste composting method Download PDFInfo
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Abstract
The invention discloses a complex functional microbial inoculum for composting fermentation and a kitchen waste composting method. The compound functional microbial inoculum comprises the following raw materials in parts by weight: 5-15 parts of bacillus thuringiensis liquid, 5-15 parts of bacillus cereus liquid, 3-10 parts of bacillus amyloliquefaciens liquid, 1-8 parts of pseudomonas putida liquid, 10-20 parts of aquatic comamonas liquid, 5-10 parts of azospirillum liquid and 3-10 parts of streptomycete liquid. According to the invention, the bacillus thuringiensis liquid, the bacillus cereus liquid, the bacillus amyloliquefaciens liquid, the pseudomonas putida liquid, the azospirillum liquid and the streptomycete liquid are compounded to prepare the functional fermentation microbial inoculum, and the specific functional fermentation microbial inoculum can timely, efficiently and environmentally treat kitchen garbage to prepare an organic fertilizer, so that the organic fertilizer has high economic benefit, and has wide development space and good market prospect.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a compound functional microbial inoculum for composting fermentation and a kitchen waste composting method.
Background
Environmental pollution and energy shortage are becoming serious and serious, and become two major problems facing the world today. The kitchen waste is one of the biological wastes with the largest global yield, has higher water content and organic matter content, and has wide attention on resource utilization. At present, a great deal of research reports are brought forward at home and abroad about the kitchen waste composting technology. However, the kitchen waste contains not only a large amount of organic substances such as protein, starch, and grease, but also a relatively high salt content. It is important to note that the high content of salt in the kitchen waste has a great inhibitory effect on the activity and degradation capability of the microbial agents on the market at present, and the application of the microbial composting technology is also restricted to a great extent.
Disclosure of Invention
The invention aims to provide a complex functional microbial inoculum for composting fermentation and a kitchen waste composting method, so as to solve the technical problems.
In order to achieve the purpose, the technical scheme of the invention is as follows:
in a first aspect, the invention provides a compound functional microbial inoculum for compost fermentation, which comprises the following raw materials in parts by weight: 5-15 parts of bacillus thuringiensis liquid, 5-15 parts of bacillus cereus liquid, 3-10 parts of bacillus amyloliquefaciens liquid, 1-8 parts of pseudomonas putida liquid, 10-20 parts of aquatic comamonas liquid, 5-10 parts of azospirillum liquid and 3-10 parts of streptomycete liquid.
Preferably, the compound functional microbial inoculum for compost fermentation comprises the following raw materials in parts by weight: 7-13 parts of bacillus thuringiensis liquid, 6-10 parts of bacillus cereus liquid, 5-8 parts of bacillus amyloliquefaciens liquid, 3-6 parts of pseudomonas putida liquid, 14-18 parts of aquatic comamonas liquid, 6-9 parts of azospirillum liquid and 5-7 parts of streptomycete liquid.
Preferably, the compound functional microbial inoculum for composting fermentation is prepared by adopting the following method:
(1) inoculating bacillus thuringiensis strains, bacillus cereus strains, bacillus amyloliquefaciens strains, pseudomonas putida strains and azospirillum strains into a shake flask filled with an activation culture medium with the volume ratio of 20-30%, and culturing for 18-24 h under the conditions that the temperature is 35-40 ℃ and the shake flask rotating speed is 180-220 r/min, wherein the activation culture medium is as follows: peptone 2%, yeast powder 0.5%, sodium chloride 0.5%; activated bacillus thuringiensis strains, bacillus cereus strains, bacillus amyloliquefaciens strains, pseudomonas putida strains and azospirillum strains are subjected to three times of gradient culture respectively, and the conditions of each time of gradient culture are as follows: the temperature is 28-32 ℃, the stirring speed is 200-220 r/min, the culture time is 6-24 h, the first gradient culture medium is a broth culture medium with the salt content of 2-3%, the second gradient culture medium is a broth culture medium with the salt content of 3-4%, the third gradient culture medium is a broth culture medium with the salt content of 4-5%, and bacillus thuringiensis liquid, bacillus cereus liquid, bacillus amyloliquefaciens liquid, pseudomonas putida liquid and spirospirillum azotobacter liquid are obtained through culture;
(2) inoculating a streptomyces strain into a shake flask filled with an activation culture medium with the volume ratio of 20-30%, and culturing for 12-24 h under the conditions that the temperature is 35-40 ℃ and the shake flask rotating speed is 180-220 r/min, wherein the activation culture medium is a glucose potato culture medium; performing gradient culture on the activated streptomycete strain for three times, wherein the conditions of each gradient culture are as follows: the temperature is 25-30 ℃, the stirring speed is 220-240 r/min, the culture time is 10-24 h, the first gradient culture medium is a glucose potato culture medium with the salt content of 2-3%, the second gradient culture medium is a glucose potato culture medium with the salt content of 3-4%, the third gradient culture medium is a glucose potato culture medium with the salt content of 4-5%, and streptomycete bacterial liquid is obtained through culture;
(3) and mixing the bacillus thuringiensis bacterial liquid, the bacillus cereus bacterial liquid, the bacillus amyloliquefaciens bacterial liquid, the pseudomonas putida bacterial liquid, the azospirillum bacterial liquid and the streptomycete bacterial liquid to obtain the compound functional microbial inoculum.
Further preferably, the first gradient culture medium, the second gradient culture medium and the third gradient culture medium of step (1) are prepared by the following methods respectively: adding sodium chloride into the meat soup culture medium according to the salt content, and mixing uniformly to obtain the product.
Further preferably, the first gradient culture medium, the second gradient culture medium and the third gradient culture medium of step (2) are prepared by the following methods respectively: adding sodium chloride into the meat soup culture medium according to the salt content, and mixing uniformly to obtain the product.
Preferably, the streptomyces is streptomyces albidoflauvs.
In a second aspect, the invention provides a kitchen waste composting method, comprising the following steps:
(1) draining the kitchen waste until the water content is 50-65%, adding straw powder, and uniformly mixing to obtain a compost;
(2) preparing a built functional microbial inoculum according to the first aspect;
(3) fully mixing the compound functional microbial inoculum with the compost, performing compost fermentation, and turning the compost every 3-5 days;
(4) and after composting for 12-15 days, spraying the mixed functional microbial inoculum and water onto compost every 3-5 days, and turning the compost for 25-40 days.
Preferably, the straw powder in the step (1) is selected from any one or more of corn straw powder, rice straw powder, wheat straw powder and sorghum straw powder.
Preferably, the weight ratio of the straw powder in the step (3) to the compost is 0.1-0.5: 1.
Preferably, the weight ratio of the compound functional microbial inoculum in the step (3) to the compost is 0.001-0.1: 1.
Preferably, the weight ratio of the compound functional microbial inoculum to the compost in the step (4) is 0.01-0.02: 1.
Preferably, the water content of the compost in the step (4) is 55-65%.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, the bacillus thuringiensis liquid, the bacillus cereus liquid, the bacillus amyloliquefaciens liquid, the pseudomonas putida liquid, the azospirillum liquid and the streptomycete liquid are compounded to prepare the functional fermentation microbial inoculum, and the specific functional fermentation microbial inoculum can timely, efficiently and environmentally treat kitchen garbage to prepare an organic fertilizer, so that the organic fertilizer has high economic benefit, and has wide development space and good market prospect.
Detailed Description
The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
It should be noted that the Streptomyces albugineus was used in the examples and comparative examples.
Example 1
The preparation method of the compound functional microbial inoculum for compost fermentation provided by the embodiment comprises the following steps:
(1) inoculating bacillus thuringiensis strains, bacillus cereus strains, bacillus amyloliquefaciens strains, pseudomonas putida strains and azospirillum strains into a shake flask filled with an activation culture medium with a volume ratio of 25%, and culturing for 22h under the conditions that the temperature is 35-40 ℃ and the shake flask rotating speed is 200r/min, wherein the activation culture medium is as follows: peptone 2%, yeast powder 0.5%, sodium chloride 0.5%; activated bacillus thuringiensis strains, bacillus cereus strains, bacillus amyloliquefaciens strains, pseudomonas putida strains and azospirillum strains are subjected to three times of gradient culture respectively, and the conditions of each time of gradient culture are as follows: the temperature is 28-32 ℃, the stirring speed is 200r/min, the culture time is 12 hours, the first gradient culture medium is a broth culture medium with the salt content of 2.5%, the second gradient culture medium is a broth culture medium with the salt content of 3.5%, the third gradient culture medium is a broth culture medium with the salt content of 4%, and the first gradient culture medium, the second gradient culture medium and the third gradient culture medium are obtained by adding sodium chloride into the broth culture medium according to the salt content and uniformly mixing, and culturing is carried out to obtain a bacillus thuringiensis liquid, a bacillus cereus liquid, a bacillus amyloliquefaciens liquid, a pseudomonas putida liquid and a azospirillum liquid;
(2) inoculating a streptomyces strain into a shake flask filled with an activation culture medium with a volume ratio of 25%, and culturing for 16h under the conditions that the temperature is 35-40 ℃ and the shake flask rotating speed is 180r/min, wherein the activation culture medium is a glucose potato culture medium; performing gradient culture on the activated streptomycete strain for three times, wherein the conditions of each gradient culture are as follows: the temperature is 25-30 ℃, the stirring speed is 220r/min, the culture time is 14 hours, the first gradient culture medium is a glucose potato culture medium with the salt content of 2%, the second gradient culture medium is a glucose potato culture medium with the salt content of 3%, the third gradient culture medium is a glucose potato culture medium with the salt content of 4%, and the first gradient culture medium, the second gradient culture medium and the third gradient culture medium are obtained by adding sodium chloride into a meat soup culture medium according to the salt content and uniformly mixing to obtain streptomycete bacteria liquid through culture;
(3) weighing the following bacteria liquid in parts by weight: 10 parts of bacillus thuringiensis bacterial liquid, 80 parts of bacillus cereus bacterial liquid, 6 parts of bacillus amyloliquefaciens bacterial liquid, 5 parts of pseudomonas putida bacterial liquid, 16 parts of comamonas aquatic bacterial liquid, 7 parts of azospirillum bacterial liquid and 6 parts of streptomycete bacterial liquid, and all the bacterial liquids are mixed to obtain the compound functional microbial inoculum.
The kitchen waste composting method provided by the embodiment comprises the following steps:
(1) draining the kitchen waste to a water content of 50-65%, adding straw powder, and uniformly mixing to obtain a compost, wherein the weight ratio of the straw powder to the compost is 0.2: 1;
(2) adopting the compound functional microbial inoculum obtained by the embodiment;
(3) fully mixing the compound functional microbial inoculum with the compost, performing compost fermentation, and turning the compost every 3 days, wherein the weight ratio of the compound functional microbial inoculum to the compost is 0.02: 1;
(4) and after composting for 12 days, spraying the mixed functional microbial inoculum and water onto the compost every 3 days, turning the compost to ensure that the water content of the compost is 55-65%, and composting until 36 days to finish composting, wherein the weight ratio of the mixed functional microbial inoculum to the compost is 0.01: 1.
Example 2
Repeating the steps (1) to (2) of the example 1, and weighing the following bacterial liquid in parts by weight: 7 parts of bacillus thuringiensis bacterial liquid, 6 parts of bacillus cereus bacterial liquid, 5 parts of bacillus amyloliquefaciens bacterial liquid, 3 parts of pseudomonas putida bacterial liquid, 18 parts of comamonas aquatic bacterial liquid, 9 parts of azospirillum bacterial liquid and 7 parts of streptomycete bacterial liquid, and all the bacterial liquids are mixed to obtain the compound functional microbial inoculum.
The kitchen waste composting method provided by the embodiment comprises the following steps:
(1) draining the kitchen waste to a water content of 50-65%, adding straw powder, and uniformly mixing to obtain a compost, wherein the weight ratio of the straw powder to the compost is 0.2: 1;
(2) adopting the compound functional microbial inoculum obtained by the embodiment;
(3) fully mixing the compound functional microbial inoculum with the compost, performing compost fermentation, and turning the compost every 5 days, wherein the weight ratio of the compound functional microbial inoculum to the compost is 0.02: 1;
(4) and after composting for 15 days, spraying the mixed functional microbial inoculum and water onto the compost every 5 days, turning the compost to ensure that the water content of the compost is 55-65%, and composting until 35 days to finish composting, wherein the weight ratio of the mixed functional microbial inoculum to the compost is 0.01: 1.
Example 3
Repeating the steps (1) to (2) of the example 1, and weighing the following bacterial liquid in parts by weight: 13 parts of bacillus thuringiensis bacterial liquid, 10 parts of bacillus cereus bacterial liquid, 8 parts of bacillus amyloliquefaciens bacterial liquid, 6 parts of pseudomonas putida bacterial liquid, 14 parts of comamonas aquatic bacterial liquid, 6 parts of azospirillum bacterial liquid and 5 parts of streptomycete bacterial liquid, and all the bacterial liquids are mixed to obtain the compound functional microbial inoculum.
The kitchen waste composting method provided by the embodiment comprises the following steps:
(1) draining the kitchen waste to a water content of 50-65%, adding straw powder, and uniformly mixing to obtain a compost, wherein the weight ratio of the straw powder to the compost is 0.2: 1;
(2) adopting the compound functional microbial inoculum obtained by the embodiment;
(3) fully mixing the compound functional microbial inoculum with the compost, performing compost fermentation, and turning the compost every 4 days, wherein the weight ratio of the compound functional microbial inoculum to the compost is 0.01: 1;
(4) and after composting for 12 days, spraying the mixed functional microbial inoculum and water onto the compost every 5 days, turning the compost to ensure that the water content of the compost is 55-65%, and composting until 40 days to finish composting, wherein the weight ratio of the mixed functional microbial inoculum to the compost is 0.02: 1.
Example 4
Repeating the steps (1) to (2) of the example 1, and weighing the following bacterial liquid in parts by weight: 8 parts of bacillus thuringiensis bacterial liquid, 7 parts of bacillus cereus bacterial liquid, 6 parts of bacillus amyloliquefaciens bacterial liquid, 4 parts of pseudomonas putida bacterial liquid, 15 parts of comamonas aquatic bacterial liquid, 7 parts of azospirillum bacterial liquid and 6 parts of streptomycete bacterial liquid, and the bacterial liquids are mixed to obtain the compound functional microbial inoculum.
The kitchen waste composting method provided by the embodiment comprises the following steps:
(1) draining the kitchen waste to a water content of 50-65%, adding straw powder, and uniformly mixing to obtain a compost, wherein the weight ratio of the straw powder to the compost is 0.2: 1;
(2) adopting the compound functional microbial inoculum obtained by the embodiment;
(3) fully mixing the compound functional microbial inoculum with the compost, performing compost fermentation, and turning the compost every 3 days, wherein the weight ratio of the compound functional microbial inoculum to the compost is 0.015: 1;
(4) and after composting for 15 days, spraying the mixed functional microbial inoculum and water onto the compost every 4 days, turning the compost to ensure that the water content of the compost is 55-65%, and composting for 32 days to finish composting, wherein the weight ratio of the mixed functional microbial inoculum to the compost is 0.01: 1.
Example 5
Repeating the steps (1) to (2) of the example 1, and weighing the following bacterial liquid in parts by weight: 5 parts of bacillus thuringiensis bacterial liquid, 15 parts of bacillus cereus bacterial liquid, 3 parts of bacillus amyloliquefaciens bacterial liquid, 1 part of pseudomonas putida bacterial liquid, 10 parts of comamonas aquatic bacterial liquid, 10 parts of azospirillum bacterial liquid and 10 parts of streptomycete bacterial liquid, and all the bacterial liquids are mixed to obtain the compound functional microbial inoculum.
The kitchen waste composting method provided by the embodiment comprises the following steps:
(1) draining the kitchen waste to a water content of 50-65%, adding straw powder, and uniformly mixing to obtain a compost, wherein the weight ratio of the straw powder to the compost is 0.2: 1;
(2) adopting the compound functional microbial inoculum obtained by the embodiment;
(3) fully mixing the compound functional microbial inoculum with the compost, performing compost fermentation, and turning the compost every 3 days, wherein the weight ratio of the compound functional microbial inoculum to the compost is 0.02: 1;
(4) and after composting for 12 days, spraying the mixed functional microbial inoculum and water onto the compost every 3 days, turning the compost to ensure that the water content of the compost is 55-65%, and composting until 36 days to finish composting, wherein the weight ratio of the mixed functional microbial inoculum to the compost is 0.01: 1.
Example 6
Repeating the steps (1) to (2) of the example 1, and weighing the following bacterial liquid in parts by weight: 15 parts of bacillus thuringiensis bacterial liquid, 5 parts of bacillus cereus bacterial liquid, 10 parts of bacillus amyloliquefaciens bacterial liquid, 8 parts of pseudomonas putida bacterial liquid, 20 parts of comamonas aquatic bacterial liquid, 5 parts of azospirillum bacterial liquid and 3 parts of streptomycete bacterial liquid, and all the bacterial liquids are mixed to obtain the compound functional microbial inoculum.
The kitchen waste composting method provided by the embodiment comprises the following steps:
(1) draining the kitchen waste to a water content of 50-65%, adding straw powder, and uniformly mixing to obtain a compost, wherein the weight ratio of the straw powder to the compost is 0.2: 1;
(2) adopting the compound functional microbial inoculum obtained by the embodiment;
(3) fully mixing the compound functional microbial inoculum with the compost, performing compost fermentation, and turning the compost every 3 days, wherein the weight ratio of the compound functional microbial inoculum to the compost is 0.02: 1;
(4) and after composting for 12 days, spraying the mixed functional microbial inoculum and water onto the compost every 3 days, turning the compost to ensure that the water content of the compost is 55-65%, and composting until 36 days to finish composting, wherein the weight ratio of the mixed functional microbial inoculum to the compost is 0.01: 1.
Comparative example 1
The preparation method of the compound functional microbial inoculum for composting fermentation provided by the comparative example comprises the following steps:
(1) inoculating bacillus thuringiensis strains, bacillus cereus strains, bacillus amyloliquefaciens strains, pseudomonas putida strains and azospirillum strains into a shake flask filled with an activation culture medium with a volume ratio of 25%, and culturing for 22h under the conditions that the temperature is 35-40 ℃ and the shake flask rotating speed is 200r/min, wherein the activation culture medium is as follows: peptone 2%, yeast powder 0.5%, sodium chloride 0.5%; respectively putting activated bacillus thuringiensis strains, bacillus cereus strains, bacillus amyloliquefaciens strains, pseudomonas putida strains and azospirillum strains into a broth culture medium with salt content of 4%, mixing acetic acid and lactic acid according to a weight ratio of 1:3 according to the salt content, adding the mixture into a meat soup culture medium, uniformly mixing, adding sodium chloride, and uniformly mixing to obtain the bacillus thuringiensis strain; the culture conditions were: culturing at 28-32 ℃ and at a stirring speed of 200r/min for 12h to obtain a bacillus thuringiensis bacterial liquid, a bacillus cereus bacterial liquid, a bacillus amyloliquefaciens bacterial liquid, a pseudomonas putida bacterial liquid and a azospirillum bacterial liquid;
(2) inoculating a streptomyces strain into a shake flask filled with an activation culture medium with a volume ratio of 25%, and culturing for 16h under the conditions that the temperature is 35-40 ℃ and the shake flask rotating speed is 180r/min, wherein the activation culture medium is a glucose potato culture medium; inoculating the activated streptomycete strain into a glucose potato culture medium with the salt content of 4% for fermentation culture, wherein the culture conditions are as follows: the temperature is 25-30 ℃, the stirring speed is 220r/min, the culture time is 14h, the glucose potato culture medium is obtained by mixing acetic acid and lactic acid according to the weight ratio of 1:3, adding the mixture into a meat soup culture medium, uniformly mixing, adding sodium chloride, and uniformly mixing according to the salt content; culturing to obtain streptomycete bacterial liquid;
(3) weighing the following bacteria liquid in parts by weight: 10 parts of bacillus thuringiensis bacterial liquid, 80 parts of bacillus cereus bacterial liquid, 6 parts of bacillus amyloliquefaciens bacterial liquid, 5 parts of pseudomonas putida bacterial liquid, 16 parts of comamonas aquatic bacterial liquid, 7 parts of azospirillum bacterial liquid and 6 parts of streptomycete bacterial liquid, and all the bacterial liquids are mixed to obtain the compound functional microbial inoculum. Composting was carried out according to the kitchen waste composting method of example 1.
Comparative example 2
In order to further illustrate the beneficial effects of the invention, according to the preparation method of the complex functional microbial inoculum for composting fermentation in the embodiment 1, the bacillus thuringiensis bacterial liquid, the bacillus cereus bacterial liquid, the bacillus amyloliquefaciens bacterial liquid and the pseudomonas putida bacterial liquid are prepared according to the step (1), and then the composting treatment is carried out according to the kitchen garbage composting method in the embodiment 1.
Comparative example 3
In order to further illustrate the beneficial effects of the invention, the complex functional microbial inoculum for composting fermentation prepared according to the preparation method of the complex functional microbial inoculum prepared in the step (1) and the step (2) in the embodiment 1 is subjected to composting treatment according to the kitchen waste composting method in the embodiment 1.
In order to further illustrate the beneficial effects of the invention, the method of the embodiments 1 to 6 and the comparative examples 1 to 3 are used for treating the kitchen waste recovered in the same batch in the same canteen, drying the product obtained after composting, controlling the moisture content to be not higher than 15%, granulating and packaging to obtain the organic fertilizer, and the nutrient content of the prepared organic fertilizer is shown in the following table after testing:
the embodiments of the present invention have been described in detail, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.
Claims (10)
1. A compound functional microbial inoculum for compost fermentation is characterized by comprising the following raw materials in parts by weight: 5-15 parts of bacillus thuringiensis liquid, 5-15 parts of bacillus cereus liquid, 3-10 parts of bacillus amyloliquefaciens liquid, 1-8 parts of pseudomonas putida liquid, 10-20 parts of aquatic comamonas liquid, 5-10 parts of azospirillum liquid and 3-10 parts of streptomycete liquid.
2. The compound functional microbial inoculum for compost fermentation of claim 1, which comprises the following raw materials in parts by weight: 7-13 parts of bacillus thuringiensis liquid, 6-10 parts of bacillus cereus liquid, 5-8 parts of bacillus amyloliquefaciens liquid, 3-6 parts of pseudomonas putida liquid, 14-18 parts of aquatic comamonas liquid, 6-9 parts of azospirillum liquid and 5-7 parts of streptomycete liquid.
3. The complex functional microbial inoculum for compost fermentation of claim 1, which is prepared by the following method:
(1) inoculating bacillus thuringiensis strains, bacillus cereus strains, bacillus amyloliquefaciens strains, pseudomonas putida strains and azospirillum strains into a shake flask filled with an activation culture medium with the volume ratio of 20-30%, and culturing for 18-24 h under the conditions that the temperature is 35-40 ℃ and the shake flask rotating speed is 180-220 r/min, wherein the activation culture medium is as follows: peptone 2%, yeast powder 0.5%, sodium chloride 0.5%; activated bacillus thuringiensis strains, bacillus cereus strains, bacillus amyloliquefaciens strains, pseudomonas putida strains and azospirillum strains are subjected to three times of gradient culture respectively, and the conditions of each time of gradient culture are as follows: the temperature is 28-32 ℃, the stirring speed is 200-220 r/min, the culture time is 6-24 h, the first gradient culture medium is a broth culture medium with the salt content of 2-3%, the second gradient culture medium is a broth culture medium with the salt content of 3-4%, the third gradient culture medium is a broth culture medium with the salt content of 4-5%, and bacillus thuringiensis liquid, bacillus cereus liquid, bacillus amyloliquefaciens liquid, pseudomonas putida liquid and spirospirillum azotobacter liquid are obtained through culture;
(2) inoculating a streptomyces strain into a shake flask filled with an activation culture medium with the volume ratio of 20-30%, and culturing for 12-24 h under the conditions that the temperature is 35-40 ℃ and the shake flask rotating speed is 180-220 r/min, wherein the activation culture medium is a glucose potato culture medium; performing gradient culture on the activated streptomycete strain for three times, wherein the conditions of each gradient culture are as follows: the temperature is 25-30 ℃, the stirring speed is 220-240 r/min, the culture time is 10-24 h, the first gradient culture medium is a glucose potato culture medium with the salt content of 2-3%, the second gradient culture medium is a glucose potato culture medium with the salt content of 3-4%, the third gradient culture medium is a glucose potato culture medium with the salt content of 4-5%, and streptomycete bacterial liquid is obtained through culture;
(3) and mixing the bacillus thuringiensis bacterial liquid, the bacillus cereus bacterial liquid, the bacillus amyloliquefaciens bacterial liquid, the pseudomonas putida bacterial liquid, the azospirillum bacterial liquid and the streptomycete bacterial liquid to obtain the compound functional microbial inoculum.
4. The complex functional microbial inoculum for compost fermentation of claim 3, wherein the first gradient culture medium, the second gradient culture medium and the third gradient culture medium of step (1) are prepared by the following methods respectively: adding sodium chloride into the meat soup culture medium according to the salt content, and mixing uniformly to obtain the product.
5. The complex functional microbial inoculum for compost fermentation of claim 3, wherein the first gradient culture medium, the second gradient culture medium and the third gradient culture medium of step (2) are prepared by the following methods respectively: adding sodium chloride into the meat soup culture medium according to the salt content, and mixing uniformly to obtain the product.
6. The complex functional microbial inoculum for compost fermentation of claim 1, wherein the streptomyces is streptomyces albidoflauvs.
7. A kitchen waste composting method is characterized by comprising the following steps:
(1) draining the kitchen waste until the water content is 50-65%, adding straw powder, and uniformly mixing to obtain a compost;
(2) preparing a compound functional microbial inoculum according to any one of claims 1-6;
(3) fully mixing the compound functional microbial inoculum with the compost, performing compost fermentation, and turning the compost every 3-5 days;
(4) and after composting for 12-15 days, spraying the mixed functional microbial inoculum and water onto compost every 3-5 days, and turning the compost for 25-40 days.
8. The kitchen waste composting method of claim 7 wherein the straw powder in step (1) is selected from any one or more of corn straw powder, rice straw powder, wheat straw powder, and sorghum straw powder.
9. The kitchen waste composting method of claim 7 wherein the weight ratio of straw powder to compost in step (3) is 0.1-0.5: 1.
10. The kitchen waste composting method as claimed in claim 7, wherein the weight ratio of the complex functional microbial inoculum in step (3) to the compost is 0.001-0.1: 1.
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| CN115448763A (en) * | 2022-07-22 | 2022-12-09 | 黄淮学院 | Microbial agent for degrading waste straws and degradation method |
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