CN108559721A - It is a kind of purification air complex micro organism fungicide and its application - Google Patents

It is a kind of purification air complex micro organism fungicide and its application Download PDF

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Publication number
CN108559721A
CN108559721A CN201810462542.9A CN201810462542A CN108559721A CN 108559721 A CN108559721 A CN 108559721A CN 201810462542 A CN201810462542 A CN 201810462542A CN 108559721 A CN108559721 A CN 108559721A
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parts
micro organism
complex micro
carrier
organism fungicide
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忻夏莹
黄国和
安春江
姚尧
张鹏
申健
陈秀娟
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Beijing Normal University
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Beijing Normal University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/38Removing components of undefined structure
    • B01D53/44Organic components
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/74General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84Biological processes
    • B01D53/85Biological processes with gas-solid contact
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14Enzymes or microbial cells immobilised on or in an inorganic carrier
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2251/00Reactants
    • B01D2251/95Specific microorganisms
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2257/00Components to be removed
    • B01D2257/70Organic compounds not provided for in groups B01D2257/00 - B01D2257/602
    • B01D2257/708Volatile organic compounds V.O.C.'s
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2258/00Sources of waste gases
    • B01D2258/06Polluted air
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Abstract

The invention discloses a kind of air purification complex micro organism fungicide and its applications.The complex micro organism fungicide includes the microorganism of following parts by weight:7 15 parts of pseudomonad, 7 15 parts of saccharomycete are rich to help 6 14 parts of bacterium, 5 12 parts of rhizobium, 4 12 parts of red spirillum, 4 12 parts of rhizopus, 29 parts of micrococcus luteus, 28 parts of bacteroid, 29 parts of clostruidium, 28 parts of Bacillus cereus, 17 parts of Bacillus alcaligenes, 17 parts of streptomycete, 17 parts of red ring bacterium, 16 parts of galactococcus, 16 parts of mycobacterium, 16 parts of Burkholderia, 16 parts of molten bacillus, 15 parts of thermophilic hydrogen bacterium, 15 parts of Xanthomonas campestris, 15 parts of shortwave monad, 0.1 3 parts of comamonas, 0.1 3 parts of Aspergillus.Above-mentioned microbial inoculum utilizes the synergistic effect of potent strain, promotes utilization and metabolic breakdown of the microorganism to volatile organic matter in air, realizes the efficient abatement of pollutant.

Description

It is a kind of purification air complex micro organism fungicide and its application
Technical field
The invention discloses technical field of air purification, it is related to a kind of complex micro organism fungicide of purification air and its answers With.
Background technology
According to statistics, the time of 80% or more modern is spent indoors, and the time is more indoors by infant, the old,weak,sick and disabled person It is long.Therefore, for the air overwhelming majority of people's breathing from interior, indoor air quality directly affects people's health.It is reported that Room air pollution has caused 35.7% breathing problem, 22% chronic lung disease and 15% tracheitis, bronchitis And lung cancer.So room air pollution has become endangers one of maximum five kinds of environmental factors to public health.
The major pollutants of room air are volatile organic matters, essentially from building and house decorative material, cook with The combustion product of smoking and other indoor activities of people.Volatile organic compounds has 350 kinds or more, existing biological Pollutant also has contaminants of chemical origin, also radioactive pollutant, it has the characteristics of diversity, accumulation property and chronicity.Room Interior environment is the space of relative closure, these pollutants can be built up indoors, and concentration is caused to increase, and constitutes the danger to human body Evil.People's most of the time is in interior,, also can be to human body in long term after human body even if the pollutant that concentration is very low Health has an adverse effect.Therefore, it takes measures to purify room air, reduces harm of the air pollution to human health As the task of top priority.
Currently, the purification method of air purifier mainly has physisorphtion, chemical reaction method, nano-photo catalytic in the market Technology, ozone anion technology, non-thermal plasma trap etc..Most of clarifier can only cross dust, pollen in air filtering etc. Substance can not remove volatile organic matter.Even if volatile organic matter is adsorbed by adsorbent fixation, just lost after adsorption saturation Effectiveness is gone, if replacing not in time, and can be resolved in air.Although ozone can strengthen photochemical catalytic oxidation decomposing pollutant Effect, but itself be also air pollutants, it be easy to cause secondary pollution.Along with most air purifiers need periodically more Adsorbent or internal setting are changed, causes its maintenance expense expensive.Air purifier using plant purification and biodegradation technique exists Vehicles Collected from Market proportion is minimum, but due to its environmental protection, naturally, secondary pollution is neither will produce, and can be in the same of purification air When achieve the effect that beautify the environment, following development prospect is very wide.
The shortcomings that existing plant purification device technology, is that the indigenous microorganism in soil will undergo the selected of a period of time The preferable pollutant removal effect of process competence exertion selected, adapt to and tamed.Directly using micro- with higher degrading activity Biological inoculum gives full play to the spy of the efficient of complex micro organism fungicide, low consumption, self-reproduction and mutually beneficial collaboration using nutriment Property, processing capacity and efficiency to volatile organic matter will be greatly improved.In addition, additional potent microbes and the micro- life of original inhabitants Object forms competitive relation, it is possible to influencing its quantity and activity.In this way, it is necessary to which immobilized microorganism technology will be efficiently micro- On carrier, formation is effectively protected it for biological fixation.And existing microorganism technique for fixing is in biocompatibility and to dirt Still there is prodigious deficiency in terms of the adsorption capacity for contaminating object.
Invention content
In order to solve microorganism technique for fixing existing in the prior art in biocompatibility and to the adsorption energy of pollutant Hypodynamic problem, the present invention provide a kind of air purification complex micro organism fungicide and its application in air purification process.
For this purpose, the technical scheme is that:
A kind of complex micro organism fungicide of purification air, includes the microorganism of following parts by weight:7-15 parts of pseudomonad, ferment Female bacterium 7-15 parts, rich to help 6-14 parts of bacterium, 5-12 parts of rhizobium, red spirillum 4-12 parts, 4-12 parts of rhizopus, 2-9 parts of micrococcus luteus is intended 2-8 parts of bacillus, 2-9 parts of clostruidium, 2-8 parts of Bacillus cereus, 1-7 parts of Bacillus alcaligenes, 1-7 parts of streptomycete, red ring 1-7 parts of bacterium, 1-6 parts of galactococcus, 1-6 parts of mycobacterium, 1-6 parts of Burkholderia, molten bacillus 1-6 parts, 1-5 parts of thermophilic hydrogen bacterium, Huang Dan 1-5 parts of born of the same parents bacterium, 1-5 parts of shortwave monad, 0.1-3 parts of comamonas, 0.1-3 parts of Aspergillus;The complex micro organism fungicide Unit volume in viable count be not less than 108cells/mL。
Above-mentioned complex micro organism fungicide, it is preferred that include the microorganism of following parts by weight:10-13 parts of pseudomonad, yeast 10-12 parts of bacterium, rich to help 9-11 parts of bacterium, 8-10 parts of rhizobium, red spirillum 6-10 parts, 6-10 parts of rhizopus, 3-7 parts of micrococcus luteus is intended 4-6 parts of bacillus, 3-7 parts of clostruidium, 4-6 parts of Bacillus cereus, 2-5 parts of Bacillus alcaligenes, 2-5 parts of streptomycete, red ring 2-5 parts of bacterium, 2-4 parts of galactococcus, 2-4 parts of mycobacterium, 2-4 parts of Burkholderia, molten bacillus 2-4 parts, 2-3 parts of thermophilic hydrogen bacterium, Huang Dan 2-3 parts of born of the same parents bacterium, 2-3 parts of shortwave monad, 0.5-1.5 parts of comamonas, 0.5-1.5 parts of Aspergillus;
Preferably, the viable count in the unit volume of the complex micro organism fungicide is 108-1011cells/mL。
Above-mentioned parts by weight are the wet bacterium re-computations by various microorganisms.In above-mentioned complex micro organism fungicide, each micro- life In object:
The pseudomonad (pseudomonadaceae) is optional but be not limited to, Pseudomonas fluorescens, P. aeruginosa One or more in bacterium, pseudomonas putida, Pseudomonas stutzeri.
The saccharomycete (saccharomycetes), it is optional but be not limited to, brewer's yeast, Hansenula yeast, torulopsis, One or more in Candida, Pichia pastoris, rhodotorula, cotton disease needle spore yeast, saccharomyces uvarum bacterium.
The rhizobium (Rhizobiaceae) are optional but be not limited to, rihizobium japonicum, rhizobium leguminosarum, alfalfa Phylloxera One or more in bacterium, crowtoe tumor bacterium, root of hair rhizobium, Rhizobium of Milk Vetch.
The red spirillum (Rhodospirillum), optional but be not limited to, Rhodospirillum rubrum, needs salt red at the red spirillum of outer sulphur One or more in spirillum.
The rhizopus (Rhizopus oryzae) is optional but be not limited to, Rhizopus oryzae, rhizopus chinensis bacterium, Rhizopus arrhizus One or more in bacterium, Rhizopus oligosporus rhizopus stolonifer bacterium, rhizopus niger.
The micrococcus luteus (micrococcus) is optional but be not limited to, micrococcus luteus, rosiness microballoon, micrococcus varians In one or more.
The bacteroid (Bacteroidaceae), optional but be not limited to, bacteroides vulgatus, bacteroides uniformis, multiform are quasi- One or more in bacillus.
The clostruidium (clostridia) is optional but be not limited to, clostridium difficile or butyric acid fusiform Bacillus.
The Bacillus alcaligenes (alcaligenes), optional but be not limited to, Bacillus foecalis alkaligenes or xylose aoxidize Bacillus alcaligenes.
The streptomycete (streptomycete), optional but be not limited to, streptomyces microflavus, streptomyces griseus, white strepto- Bacterium, streptomyces mobaraensis, streptomyces hygroscopicus, streptomyces erythareus, Avidin streptomycete, styreptomyces globispotus strain, the brown streptomycete of color, cracking One or more in streptomycete.
The red ring bacterium (rhodocyclaceae) is optional but be not limited to, deep red red ring bacterium.
The galactococcus (lactococcus) is optional but be not limited to, Lactococcus lactis, Lactococcus garvieae.
The mycobacterium (mycobacterium) be non-pathogenic mycobacterium or opportunistic mycobacterium, can It selects but is not limited to, in bog moss mycobacteria, Mycobacterium graminis, external mycobacteria, thousand field mycobacterias, purplish red mycobacteria One or more.
The Burkholderia (Burkholderiales), it is optional but be not limited to, soil Burkholderia, grignard Burkholderia, One or more in onion Burkholderia.
The molten bacillus (Lysobacter), it is optional but be not limited to, become the molten bacillus of palm fibre, the molten bacillus of capsicum, the molten bacillus in Xinjiang, The molten bacillus of producing enzyme, Xi Zangxi rub molten bacillus, the molten bacillus of auspicious body, the molten bacillus of anti-arsenic, the molten bacillus of condensation, occupy the molten bacillus of sponge, particle One or more in the molten bacillus of shape, the molten bacillus of antibiotic, gluey molten bacillus.
The thermophilic hydrogen bacterium (hydrogenophilaceae), optional but be not limited to, intermediate thermophilic hydrogen bacterium.
The Xanthomonas campestris (xanthomonas), it is optional but be not limited to, xanthomonas campestris, translucent Xanthomonas campestris, Xanthomonas maltophilia, rice Xanthomonas, xanthomonas albilineans, grape wine Xanthomonas campestris, Xanthomonas axonopodis, grass are careless yellow One or more in monad.
The shortwave monad (Brevundimonas) is optional but be not limited to, pseudomonas diminuta or pseudomonas vesicularis In one or more.
The comamonas (Comamonas) is optional but be not limited to, Comamonas terrigena, C testosteroni unit cell One or more in bacterium, compost comamonas, aquatic comamonas.
The Aspergillus (Aspergillus), it is optional but be not limited to, black-koji mould, Aspergillus ochraceus, cinnamon aspergillus, without flower One or more in fruit aspergillus, aspergillus oryzae, Aspergillus sojae.
In above-mentioned complex micro organism fungicide, in the selection of respective Pseudomonas, non-pathogenic bacteria, less preferred condition is preferentially selected to cause Germ.
In above-mentioned complex micro organism fungicide, it is preferred that the viable count in the unit volume of the complex micro organism fungicide is 108-1011cells/mL。
Further include carrier in above-mentioned complex micro organism fungicide, the microorganism is fixed on carrier, the carrier is by sticking Extrusion forming after soil, hydroxyapatite and rice husk mixing, is then sintered;The shape of the carrier is spherical, square, length Cube or other irregular shapes.
In above-mentioned complex micro organism fungicide, the mass ratio of clay, hydroxyapatite and activated carbon is 3 in the carrier: (1.5-2.5):(0.8-2)。
In above-mentioned complex micro organism fungicide, the preparation method of the carrier includes:By the clay of extra fine quality, hydroxy-apatite Stone and rice husk and water, which are uniformly mixed, obtains mixture;Then mixture is put into mold and is compacted, carrier blank is obtained after taking-up; After blank is dried, heating sintering is carried out, then cooling obtains carrier;The quality of the clay, hydroxyapatite and rice husk is total It is with the mass ratio with water:5-8:1-3.
Further include stabilizer in above-mentioned complex micro organism fungicide.
In above-mentioned complex micro organism fungicide, the stabilizer is the aqueous solution of polyvinyl alcohol and cycloheptaamylose, wherein poly- The mass ratio of vinyl alcohol, cycloheptaamylose and water is 1:(3-4):(40-50).
In above-mentioned complex micro organism fungicide, it is by the method that microorganism is fixed on carrier:Microorganism is mixed in proportion After culture, complex micro organism fungicide mother liquor is obtained;It cultivates, obtains after carrier, complex micro organism fungicide mother liquor and culture medium are mixed To the carrier of attachment complex microorganism, stabilizer is sprayed on the carrier of attachment complex microorganism, is then dried.
The preparation method of above-mentioned complex micro organism fungicide, includes the following steps:
(1) the various microorganisms in complex micro organism fungicide are subjected to single bacterium fermentation respectively;
(2) above-mentioned each single bacterium is directly mixed by specific mass ratio;Or above-mentioned bacterium is respectively cultivated to certain Quantity, that is, respectively reach certain amount (the same order of magnitude ,~104-107Cells/mL), single bacterium bacterium solution is mixed in proportion It closes, prepares complex microorganism bacterial classification liquid.Complex micro organism fungicide mother liquor is inoculated into fermentation tank and is mixed, culture is extremely Strain quantity is 108Cells/mL or more is to get to complex micro organism fungicide;
Or further,
(3) carrier, complex micro organism fungicide, culture medium are put into reaction kettle the 48-72 that ferments under the conditions of 28-35 DEG C Hour, obtain the carrier of attachment complex microorganism;
(4) stabilizer is sprayed on the carrier of attachment complex microorganism, then dries, obtains the composite microbial containing carrier Object water purification agent.
In above-mentioned preparation method, the culture medium of the mixed culture is:Following components including mass percent:Honey 0.5%, urea 0.5%, CaCl20.5%, (NH4)2SO40.1%, K2HPO40.03%, NaNO30.03%, MgSO4· 7H2O 0.02%.
In above-mentioned preparation method, the preparation method of the carrier includes:By the clay, hydroxyapatite and rice of extra fine quality Shell and water, which are uniformly mixed, obtains mixture;Then mixture is put into mold and is compacted, carrier blank is obtained after taking-up;By blank After drying, heating sintering is carried out, then cooling obtains carrier;The mass ratio of the clay, hydroxyapatite and rice husk and water is: 5-8:1-3.
In above-mentioned preparation method, the process of the heating sintering is:With 2-4 DEG C of heating rate per minute by blank from room Temperature is warming up to 370-420 DEG C, then sinters to temperature with 3-5 DEG C of heating rate heating per minute from the starting of this temperature and reach 850- It 900 DEG C, after being kept for 1 hour, is taken out from kiln after sintered body is then gradually cooling to 540-560 DEG C with 30-40 minutes.
In above-mentioned preparation method, the preparation method of the stabilizer is:Polyvinyl alcohol, cycloheptaamylose and water are mixed, Be heated with stirring to 70-75 DEG C, sodium hydroxide be added and adjusts pH to 6.8-7.2, wherein polyvinyl alcohol, cycloheptaamylose and water according to Mass ratio is 1:(3-3.6):(40-50).
A kind of air purifier contains above-mentioned complex micro organism fungicide.
Above-mentioned air purifier, including:Planting bed, the tank being arranged from top to bottom, the setting of planting bed bottom it is ventilative every Layer, planting bed are connected by the item that absorbs water with cistern.Planting bed planting plants, tank provides water source for plant, above-mentioned compound Microbial bacterial agent is respectively placed in planting bed and tank.
Above-mentioned air purifier further includes:Filler and air inlet is respectively set in tank.
The application in air purification of above-mentioned complex micro organism fungicide.
Application process of the above-mentioned complex micro organism fungicide in air purification be:Complex micro organism fungicide is added to interior In the soil and water source of planting plants, or it is added in above-mentioned air purifier.
Beneficial effects of the present invention:
(1) complex micro organism fungicide of purification air provided by the invention, is made using potent strain therein and its collaboration With the efficient abatement of pollutant is realized in utilization and metabolic breakdown of the promotion microorganism to volatile organic matter in air.Further Ground provides the barrier grown safely for high-effective microorganism by the way that complex micro organism fungicide to be fixed on carrier, alleviates outer The problem of source microorganism is to the ability of unknown environment adaptation and with the uncertain competitive relation of indigenous microorganism, and it is apparent Improve its effective concentration and activity.
(2) in the complex micro organism fungicide of purification air provided by the invention, using clay as the carrier of raw material, because it is thin Degree is very high, and plasticity is good, and hydroxyapatite and activated carbon increase the biocompatibility of carrier, and microorganism is made to be more easy to be attached to it Surface, and strengthen the absorption to pollutant;In conjunction with the control to sintering temperature and time, to ensure that carrier has large hole Gap rate, no rhegma and have enough compression strength.Meanwhile the raw material sources of carrier are abundant, and it is cheap, greatly reduce carrier Cost of manufacture, safe and non-toxic, recyclable recycling not will produce secondary pollution.
Biocompatible materials and activated carbon pore creating material are added through the invention, are set specific sintering temperature, can be made the load Body has higher porosity, is conducive to the apposition growth of more microorganism, while strengthening absorption of the carrier to pollutant, favorably Utilization is decomposed to it in microorganism;The carrier material of the present invention is safe and non-toxic, and recyclable recycling not will produce secondary pollution. It is raw material with clay, activated carbon is that pore creating material prepares microbial carrier, and the good crushing resistance of carrier may be implemented, higher Porosity, and to the stronger absorption of organic pollution.Hydroxyapatite has excellent biocompatibility, microorganism easily attached On the surface of hydroxyapatite, and can in stable carrier complex microbial community type and quantity.It is compound using immobilization Microbial inoculum carrier carries out the fixation and application of high-effective microorganism, you can increases the protection to high-effective microorganism, it is allowed to give full play to dirt Object removal ability is contaminated, and the absorption and fixation to pollutant can be increased, and by making full use of carrier microorganisms at many levels, original inhabitants The metabolic breakdown of microorganism and plant activity, is beneficial to height of the plant-microorganism purifier to indoor air pollutants Effect removal.
(3) air purifier provided by the invention, for the relatively low problem of indoor air pollutants removal efficiency, by setting The tank and planting bed of clarifier are set, and immobilization complex micro organism fungicide carrier is respectively placed in Shuan Chizhong, at many levels Carrier microorganisms, indigenous microorganism, or the further metabolic breakdown activity using plant are made full use of, dirt is further realized Contaminate the efficient removal of object.
To the tank and planting bed by the way that clarifier is arranged, and immobilization complex micro organism fungicide carrier is placed respectively In Yu Shuanchi, makes full use of the metabolic breakdown of carrier microorganisms and plant active at many levels, further realize the height of pollutant Effect removal.
Description of the drawings
Fig. 1 is air purifier structural schematic diagram, wherein in figure:1- air inlets, 2- immobilization complex micro organism fungicides carry Body, 3- planting beds, 4- fan powers wiring hole, 5- tanks, 6- immobilization complex micro organism fungicides carrier, 7- water suctions item, 8- Ventilating interlayer, the filler 9-, 10- plants, 11- wind turbines.
Specific implementation mode
With reference to specific embodiment, the present invention will be further described, but the scope of protection of present invention is not limited to In the range of embodiment statement, anyone can obtain other various forms of products under the inspiration of the present invention, however, Make any variation in its shape or component ratio or Plants types choice, it is every that there is technology identical or similar to the present application Scheme is all fallen in protection scope of the present invention.Used original and instrument is unless otherwise specified in the embodiment of the present invention It is commercially available.
Embodiment 1
The complex micro organism fungicide of the purification air of the present invention, includes the microorganism of following parts by weight:10 parts of pseudomonad, 10 parts of saccharomycete, it is rich to help 11 parts of bacterium, 8 parts of rhizobium, 6 parts of red spirillum, 6 parts of rhizopus, 7 parts of micrococcus luteus, 6 parts of bacteroid, fusiform 6 parts of bacillus, 4 parts of Bacillus cereus, 3 parts of Bacillus alcaligenes, 2 parts of streptomycete, 3 parts of red ring bacterium, 2 parts of galactococcus, branch bar 2 parts of bacterium, 3 parts of Burkholderia, 2 parts of molten bacillus, 3 parts of thermophilic hydrogen bacterium, 2 parts of Xanthomonas campestris, 2 parts of shortwave monad, comamonas 1 Part, 1 part of Aspergillus;Above-mentioned parts by weight are the wet bacterium re-computations by various microorganisms;The unit bodies of the complex micro organism fungicide Viable count in product is at least 108cells/mL。
The preparation method of the complex micro organism fungicide of above-mentioned purification air is:
(1) each single bacterium is respectively enlarged culture, culture to each single bacterium concentration respectively reaches~104- 105Under conditions of cells/mL, single bacterium bacterium solution is obtained;
(2) above-mentioned each single bacterium bacterium solution is inoculated into the fermentation tank equipped with fermentation medium according to aforementioned proportion, inoculum concentration Be the 5% of culture volume, cultivation temperature is 33 DEG C, is fully contacted with nutriment using stirring, when viable count up to 5 × 108To get to complex micro organism fungicide when cells/mL or more.
Wherein, fermentation medium includes honey 0.5%, urea 0.5%, CaCl20.5%, (NH4)2SO40.1%, K2HPO40.03%, NaNO30.03%, MgSO4·7H2O0.02%.
Embodiment 2
The complex micro organism fungicide of the purification air of the present invention, includes the microorganism of following parts by weight:12 parts of pseudomonad, 12 parts of saccharomycete, it is rich to help 10 parts of bacterium, 9 parts of rhizobium, 8 parts of red spirillum, 8 parts of rhizopus, 4 parts of micrococcus luteus, 5 parts of bacteroid, fusiform 3 parts of bacillus, 4 parts of Bacillus cereus, 2 parts of Bacillus alcaligenes, 2 parts of streptomycete, 2 parts of red ring bacterium, 2 parts of galactococcus, branch bar 4 parts of bacterium, 2 parts of Burkholderia, 2 parts of molten bacillus, 3 parts of thermophilic hydrogen bacterium, 2 parts of Xanthomonas campestris, 2 parts of shortwave monad, comamonas 1 Part, 1 part of Aspergillus;Above-mentioned parts by weight are the wet bacterium re-computations by various microorganisms;The unit bodies of the complex micro organism fungicide Viable count in product is at least 108cells/mL。
The preparation method of the complex micro organism fungicide of above-mentioned purification air is:
(1) each single bacterium is respectively enlarged culture, culture to each single bacterium concentration respectively reaches~106- 107Under conditions of cells/mL, single bacterium bacterium solution is obtained;
(2) above-mentioned each single bacterium bacterium solution is inoculated into the fermentation tank equipped with fermentation medium according to aforementioned proportion, inoculum concentration Be the 5% of culture volume, cultivation temperature is 33 DEG C, is fully contacted with nutriment using stirring, when viable count up to 5 × 108To get to complex micro organism fungicide when cells/mL or more.
Wherein, fermentation medium includes honey 0.5%, urea 0.5%, CaCl20.5%, (NH4)2SO40.1%, K2HPO40.03%, NaNO30.03%, MgSO4·7H2O0.02%.
Embodiment 3
The complex micro organism fungicide of the purification air of the present invention further includes carrier on the basis of embodiment 1.
Preparation method is:
(1) carrier is prepared:
(1) by clay (80 mesh), hydroxyapatite, activated carbon (250 mesh) and water are according to mass ratio 3:2:1:2 are put into It is uniformly mixed in dough mixing machine;After mixing, mixture is put into spherical die and is compacted, taken out after 2 minutes spherical Carrier blank, diameter 2cm;
(2) carrier blank is put into after natural drying in kiln, with 2 DEG C of heating rates per minute by blank from room temperature 370 DEG C are warming up to, then sinters to temperature with 3 DEG C of heating rates heatings per minute from the starting of this temperature and reaches 850 DEG C, keeps 1 After hour, takes out, then Temperature fall, obtain from kiln after sintered body is then gradually cooling to 540 DEG C with 30-40 minutes The carrier.
(2) complex micro organism fungicide containing carrier is prepared:
Complex micro organism fungicide prepared by embodiment 1 as the carrier of complex microorganism bacterium solution mother liquor and above-mentioned preparation, Culture medium is put into reaction kettle without chlorine water and ferments 48 hours under the conditions of 33 DEG C, makes carrier abundant with culture medium using stirring Contact, allows microorganism adequately to adhere on carrier, and the carrier of load complex micro organism fungicide is obtained after taking-up.
By polyvinyl alcohol, cycloheptaamylose and water according to mass ratio 1:3:43 are added in container, are heated with stirring to 70 DEG C, And appropriate sodium hydroxide is added and adjusts pH to 7.0, prepare stabilizer;
The carrier of the load complex micro organism fungicide of above-mentioned preparation is put into dough mixing machine and is stirred, while uniformly sprinkling is stablized Agent, then dried, obtain immobilization complex micro organism fungicide.
Embodiment 4
The complex micro organism fungicide of the purification air of the present invention further includes carrier on the basis of embodiment 2.
Preparation method is:
(1) carrier is prepared:
(1) clay (80 mesh) is taken, hydroxyapatite, activated carbon (100 mesh) and water are according to mass ratio 3:1.8:1.4:2.5 It is put into dough mixing machine and is uniformly mixed;The mixture of certain mass after mixing, is being taken to be put into compacting in rectangular die, Rectangle carrier blank is taken out after 2 minutes;
(2) blank is put into after natural drying in kiln, with 3 DEG C of heating rates per minute by blank from room temperature To 420 DEG C, then temperature is sintered to 4 DEG C of heating rates heatings per minute from the starting of this temperature and reach 900 DEG C, kept for 1 hour Afterwards, it takes out, then Temperature fall, is carried from kiln after sintered body being then gradually cooling to 560 DEG C with 30-40 minutes Body;
(2) complex micro organism fungicide containing carrier is prepared:
Using complex micro organism fungicide prepared by embodiment 2 as complex microorganism bacterium solution mother liquor with by the above-mentioned load being fired into Body, culture medium are put into reaction kettle without chlorine water and ferment 72 hours under the conditions of 28 DEG C, are fully connect with nutriment using stirring It touches, microorganism is allowed adequately to adhere on carrier, the carrier of load complex micro organism fungicide is obtained after taking-up;
By polyvinyl alcohol, cycloheptaamylose and water are according to mass ratio 1:3.3:47 are added in container, are heated with stirring to 75 DEG C, and appropriate sodium hydroxide is added and adjusts pH to 7.2, prepare stabilizer;
Stabilizer is uniformly sprayed in the carrier surface of the load complex micro organism fungicide of above-mentioned preparation, then is dried, is obtained To immobilization complex micro organism fungicide.
Embodiment 5
The air purifier of the present invention is as shown in Figure 1, include:Planting bed 3, the tank 5 being arranged from top to bottom, planting bed Ventilating interlayer 8 is arranged in 3 bottoms, and planting bed logical 3 crosses water suction item 7 and is connected with tank 5.3 kinds of planting bed plants bracketplant or sansevieria trifasciata, storage Pond 5 provides water source for plant.
Above-mentioned air purifier further includes:Filler 9, air inlet 1 and fan power wiring hole is respectively set in tank 5 4。
Immobilization complex micro organism fungicide carrier prepared by embodiment 3 is respectively put into tank 5 and the plantation of clarifier Layer 3, is uniformly dispersed;
Or the immobilization complex micro organism fungicide carrier for preparing embodiment 4, it is respectively put into 5 He of tank of clarifier Layer 3 is planted, is uniformly dispersed;
Clarifier is connected into wind turbine 11 by fan power wiring hole 4, after wind turbine starts, the tank of air suction device It is interior, the pollutant in air be immobilized after complex micro organism fungicide carrier adsorption, and the utilization that is further degraded by microorganisms. Periodically add water into tank, and 5% nutrient solution or culture medium is added, to meet the nutrition needed for immobilized microorganism growth Ingredient, required nutrient solution include honey 0.2%, urea 0.3%, CaCl20.3%, (NH4)2SO40.05%, K2HPO4 0.015%, NaNO30.015%, MgSO4·7H2O0.01%.
Water suction upper end in clarifier is inserted into the soil of planting bed, and tank is immersed in lower end across ventilating interlayer It is interior, it is that bracketplant or sansevieria trifasciata body feed moisture and nutriment by capillary phenomenon.Meanwhile the pollutant of degradation not to be adsorbed with Moisture enters in soil, the complex micro organism fungicide carrier adsorption after being immobilized, and further by the microorganism on carrier, soil Indigenous microorganism and bracketplant degradation in earth utilize, and improve purification efficiency.
The method that the present invention implements to provide is by complex micro organism fungicide culture, is fixed on carrier, then passes through stabilization Microbial inoculum is set to keep a greater number and greater activity after agent processing.It is again that the immobilized microorganism microbial inoculum is net for plant-microorganism Change a kind of combination technique of device.
The technology is the fixation and slow release effect by immobilized microorganism microbial inoculum to high-efficiency strain, improves microbe carrier The effective concentration and bioactivity of complex micro organism fungicide in area enable microorganism quickly to form efficient biology on carrier Film optimizes biomembrane microecosystem structure, promotes utilization and metabolic breakdown of the microorganism to volatile organic matter in air, real The efficient abatement of existing pollutant.Meanwhile by immobilization complex micro organism fungicide carrier be respectively placed in clarifier tank and In planting bed, the metabolic breakdown activity of carrier microorganisms, indigenous microorganism and plant is taken full advantage of at many levels, further Realize the efficient removal of pollutant.

Claims (10)

1. a kind of complex micro organism fungicide of purification air, which is characterized in that include the microorganism of following parts by weight:Pseudomonad 7-15 parts, 7-15 parts of saccharomycete is rich to help 6-14 parts of bacterium, 5-12 parts of rhizobium, red spirillum 4-12 parts, 4-12 parts of rhizopus, microballoon 2-9 parts of bacterium, 2-8 parts of bacteroid, 2-9 parts of clostruidium, 2-8 parts of Bacillus cereus, 1-7 parts of Bacillus alcaligenes, streptomycete 1-7 parts, 1-7 parts of red ring bacterium, 1-6 parts of galactococcus, 1-6 parts of mycobacterium, 1-6 parts of Burkholderia, molten bacillus 1-6 parts, thermophilic hydrogen bacterium 1-5 parts, 1-5 parts of Xanthomonas campestris, 1-5 parts of shortwave monad, 0.1-3 parts of comamonas, 0.1-3 parts of Aspergillus;It is described compound Viable count in the unit volume of microbial bacterial agent is not less than 108cells/mL。
2. complex micro organism fungicide according to claim 1, which is characterized in that include the microorganism of following parts by weight:It is false 10-13 parts of monad, 10-12 parts of saccharomycete is rich to help 9-11 parts of bacterium, 8-10 parts of rhizobium, red spirillum 6-10 parts, rhizopus 6-10 Part, 3-7 parts of micrococcus luteus, 4-6 parts of bacteroid, 3-7 parts of clostruidium, 4-6 parts of Bacillus cereus, 2-5 parts of Bacillus alcaligenes, 2-5 parts of streptomycete, 2-5 parts of red ring bacterium, 2-4 parts of galactococcus, 2-4 parts of mycobacterium, 2-4 parts of Burkholderia, molten bacillus 2-4 parts, 2-3 parts of thermophilic hydrogen bacterium, 2-3 parts of Xanthomonas campestris, 2-3 parts of shortwave monad, 0.5-1.5 parts of comamonas, Aspergillus 0.5-1.5 Part;Viable count in the unit volume of the complex micro organism fungicide is 108-1011cells/mL。
3. complex micro organism fungicide according to claim 1 or 2, which is characterized in that further include carrier, by the microorganism It is fixed on carrier, then extrusion forming after the carrier is mixed by clay, hydroxyapatite and rice husk is sintered;It is described The shape of carrier is spherical, square, cuboid or other irregular shapes.
4. complex micro organism fungicide according to claim 3, which is characterized in that clay, hydroxyapatite in the carrier Mass ratio with activated carbon is 3:(1.5-2.5):(0.8-2).
5. complex micro organism fungicide according to claim 4, which is characterized in that the preparation method of the carrier includes:It will Clay, hydroxyapatite and the rice husk and water of extra fine quality are uniformly mixed and obtain mixture;Then mixture is put into mold Interior compacting obtains carrier blank after taking-up;After blank is dried, heating sintering is carried out, then cooling obtains carrier;The clay, The mass ratio of the quality summation and water of hydroxyapatite and rice husk is:5-8:1-3.
6. any one of the claim 3-5 complex micro organism fungicides, which is characterized in that further include stabilizer.
7. complex micro organism fungicide according to claim 6, which is characterized in that the stabilizer is polyvinyl alcohol and β-ring Shape dextrin, wherein polyvinyl alcohol, cycloheptaamylose mass ratio be 1:(3-4).
8. the complex micro organism fungicide described according to claim 6 or 7, which is characterized in that microorganism to be fixed on carrier Method is:After microorganism is mixed in proportion, complex micro organism fungicide mother liquor is obtained;By carrier, complex micro organism fungicide It is cultivated after mother liquor and culture medium mixing, obtains the carrier of attachment complex microorganism, sprayed on the carrier of attachment complex microorganism Then stabilizer dries.
9. a kind of air purifier, which is characterized in that contain claim 1-8 any one of them complex micro organism fungicides.
10. the application in air purification of any one of the claim 1-8 complex micro organism fungicides.
CN201810462542.9A 2018-05-15 2018-05-15 It is a kind of purification air complex micro organism fungicide and its application Pending CN108559721A (en)

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