CN104673851A - Method for preparing gamma-polyglutamic acid through soild fermentation by utilizing edible fungi residues - Google Patents
Method for preparing gamma-polyglutamic acid through soild fermentation by utilizing edible fungi residues Download PDFInfo
- Publication number
- CN104673851A CN104673851A CN201510081839.7A CN201510081839A CN104673851A CN 104673851 A CN104673851 A CN 104673851A CN 201510081839 A CN201510081839 A CN 201510081839A CN 104673851 A CN104673851 A CN 104673851A
- Authority
- CN
- China
- Prior art keywords
- solid
- polyglutamic acid
- fermentation
- gamma
- edible fungi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for preparing gamma-polyglutamic acid through solid fermentation by utilizing edible fungi residues. The method comprises the following steps: (1) preparation of solid fungicide: collecting thallus in gamma- polyglutamic acid fermentation liquor to be mixed with a carrier, and drying to obtain the solid fungicide; (2) solid fermentation: inoculating the solid fungicide obtained from the step (1) into a fermentation medium to be mixed uniformly, and fermenting to prepare the gamma-polyglutamic acid, wherein the fermentation medium consists of a solid matrix and an exogenous additive, and the solid matrix is a mixture of the edible fungi residues and aginomoto. Compared with the prior art in which gamma-polyglutamic acid is produced through solid fermentation, the method disclosed by the invention is used for efficiently producing gamma-polyglutamic acid, the production cost is greatly reduced through the utilization of agricultural waste residues, meanwhile, a new way is found for the disposal of the residues, and the environment pollution problem caused by the residues is avoided. Therefore, the method is a high-efficient, low-cost, environment-friendly biological reaction process and has good industrial application prospect.
Description
Technical field
The invention belongs to technical field of microbial fermentation, be specifically related to a kind of method utilizing edible fungi residue solid fermentation high yield gamma-polyglutamic acid-.
Background technology
Gamma-polyglutamic acid-(γ-poly-glutamic acid is called for short γ-PGA) is that L-glutamic acid monomer is with a kind of polyamino acid (structure as follows) of γ-carboxyl with the condensation of alpha-amino group phase.Molecular weight distribution is between 100KDa to 10000KDa.
The molecular chain of γ-PGA there is the free side chain carboxyl group that a large amount of activity is higher, hydrogen bond can be formed between intramolecule or molecule, there is physics and chemistry and the biological characteristics of many uniquenesses such as splendid biodegradability, film-forming properties, one-tenth fibering, plasticity-, cohesiveness, moisture retention, focus on environmental protection, emphasize today of Sustainable development, γ-PGA and derivative thereof have very wide application prospect, can be used for makeup, food, agricultural fertilizer, medicinal dressing, dispersion agent, sequestrant, building coating, the field such as dust-proof.
At present, both at home and abroad the fermentation manufacturing technique of γ-PGA is conducted extensive research, mainly produce based on microbial liquid submerged fermentation, but still there is a lot of not enough places and insoluble problem: (1), carrying out along with fermentation, fermentation broth viscosity increases, and limits the supply of oxygen and the transmission of quality; (2), produce a large amount of foam in fermenting process, increase the weight of the probability controlling difficulty and microbiological contamination; (3), raw materials cost is expensive, greatly adds the production cost of γ-PGA; (4), water resources consumption is serious, and the north in short supply at water source especially, makes the scale operation of γ-PGA bring serious obstruction.It is carrier that solid fermentation to refer in microorganism with solid substrate, at the bioprocesses do not had or carry out under a small amount of free-water; Compared with liquid fermenting, solid fermentation has shown huge advantage, comprises that production cost is low, the energy and water resources consumption is few, equipment requirements simple, and the waste water of discharge is few.Sand is green for a long time waits people to utilize bacillus natto, is that raw material solid ferments, can produces 89.5g/kg γ-PGA (number of patent application: 200410043690.5) with soybean; The medium people of Sun Wei utilizes the method for farm and side line products solid fermentation low cost preparing gamma-PGA, and output reaches 100g/kg (patent publication No.: CN 1908178A); The human hairs such as the Chang Noboru of Qiao understand that a kind of Inert absorbent solid support material solid state fermentation produces the technique of γ-PGA, and production peak reaches 17.4g/L (patent publication No.: CN 101705260A).Feasible when the solid fermentation that more than research demonstrates γ-PGA is produced, but the problem such as it is longer to there is the production cycle, and production efficiency is low, and raw materials cost is expensive; Therefore, the method that the solid fermentation that a kind of cost is low, efficiency is high produces γ-PGA urgently is researched and developed, and this also will bring brand-new development prospect and application platform to the fermentation industry of γ-PGA!
Summary of the invention
The solid fermenting producing method of the γ-PGA that technical problem to be solved by this invention is to provide that a kind of raw materials cost is low, energy consumption is little, pollutes less, efficiency is high.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
Utilize edible fungi residue solid fermentation to produce a method for gamma-polyglutamic acid-, the method comprises the steps:
(1) preparation of solid fungicide: gamma-polyglutamic acid-is produced fermented liquid that bacterium obtains after liquid fermenting and carry out processing (such as filter or centrifugal), collect thalline, and mix with carrier, drying, obtains solid fungicide;
(2) solid fermentation: the solid fungicide that step (1) obtains is inoculated in solid fermentation substratum, mixes, proceed solid fermentation and prepare gamma-polyglutamic acid-;
Described solid fermentation substratum is made up of solid substrate and additives, and described solid substrate is the mixture of edible fungi residue and msg meal.
Wherein, in step (1), described thalline is B.subtilis PG-8, and the deposit number of this bacterial strain is CGMCC NO.6324.
Wherein, in step (1), described carrier is be ground into the wheat bran of powder and the mixture of bagasse, and wherein, the mass ratio of wheat bran and bagasse is 0.7 ~ 1.2:1 ~ 1.5, is preferably 1 ~ 1.2:1.4 ~ 1.5.
Wherein, in step (1), it is 0.8 ~ 2.4:1.5 ~ 3 that thalline and carrier add mass ratio, is preferably 1.5 ~ 2:2.2 ~ 2.8, most preferably 1.5:2.4.
Wherein, in step (1), containing viable count in described solid fungicide is 5 ~ 6.5 × 10
10cfu/g, preferably 5.0 × 10
10cfu/g.
Wherein, in step (1), described solid fungicide water content is lower than 10%, and solid fungicide water content is preferably 8%.
Wherein, in step (2), described solid fermentation, its leavening temperature is 30 ~ 35 DEG C, and fermentation time is 48 ~ 56h, every 8 hours upset cultures once.
Wherein, in step (2), the mass ratio of edible fungi residue and msg meal is 1 ~ 1.6:0.5 ~ 1, is preferably 1.3 ~ 1.5:0.5 ~ 0.6.
Wherein, described edible fungi residue is the mixture of one or more in needle mushroom dreg, flat mushroom bacterium slag, Lentinus Edodes fungus slag; The mixture of preferred Lentinus Edodes fungus slag and three kinds of edible fungi residues; The most preferably mixture of three kinds of edible fungi residues, in the mixture of further preferred three kinds of edible fungi residues, the ratio of flat mushroom bacterium slag, needle mushroom dreg, Lentinus Edodes fungus slag is 2.9 ~ 3.21:4.8 ~ 5.:5.8 ~ 6.1, preferably the ratio 3:5:6 of three kinds of flat mushroom bacterium slags, needle mushroom dreg, Lentinus Edodes fungus slag.
Wherein, in solid fermentation substratum, additives and content thereof are: inulin 150 ~ 400g/kg, corn cob meal 50 ~ 200g/kg, MnSO
4h
2o 50 ~ 200mg/kg, MgSO
47H
2o0.5 ~ 3g/kg.
Wherein, the pH of described fermention medium is 7.0, and the humidity of water use regulation solid fermentation substratum is more than 50%.
Wherein, the extraction of gamma-polyglutamic acid-and detection method as follows:
Get 5-10g fermented product, add the distilled water of 10 times of volumes, 100-200rpm stirs the centrifugal 5min of 1 ~ 1.5h, 5000rpm, and supernatant liquor adopts 0.22 μm of membrane filtration, detects with HPLC; The testing conditions of HPLC is: chromatographic column: Shodex Ohpak SB-806M HQ; Moving phase: 0.2M Na
2sO
4solution, pH 4.0 adjusted by acetic acid; Flow velocity: 1.0mL/min.
Wherein, described additives inulin prepares as follows: first gather in the crops the fast root of fresh jerusalem artichoke, and Rapid Cleaning is to remove the foreign material such as earth, Shi Sha, and in this process, the time of soaking and washing is not easily long, be no more than 30min, otherwise the loss of sugar in jerusalem artichoke can be strengthened; Then clean jerusalem artichoke is cut into block, size at about 1 ~ 3cm, and is placed in boiling water bath and heats 5min with deactivation PPO enzyme; Then the jerusalem artichoke of stripping and slicing is put into 65 DEG C of dry 8h of electric heating constant-temperature blowing drying box to water content below 15%; Pulverize finally by pulverizer, cross 40 mesh sieves, be jerusalem artichoke powder, keep for subsequent use with 4 DEG C of refrigerators.
Wherein, described additives corn cob meal obtains as follows: for subsequent use after being ground into particulate after the corn cob of threshing dries.
Beneficial effect: the present invention has following outstanding effect:
(1) the present invention is using agricultural wastes edible fungi residues as solid fermentation matrix, not only can economize on resources, low cost produces γ-PGA in large quantities, and effectively can reduce the problem of environmental pollution that bacterium slag brings.
(2) use glutamic acid fermentation waste-msg meal as the glutamate precursor of γ-PGA fermentative production in the present invention, with directly use monosodium glutamate precursor phase to compare, greatly reduce the production cost of γ-PGA, glutamic acid fermentation waste can also be processed effective and reasonablely.
(3) containing fructose, glucose and a large amount of oligofructoses in the inulin added in the present invention, for microbial growth metabolism provides a large amount of carbon sources, and inulin water absorbability is strong, can delay moisture evaporate with bacterium slag after mixing, prevent scattering and disappearing of substratum moisture.
(4) corn cob meal added in the present invention can be used as high-quality nitrogenous source, is conducive to the carrying out of subtilis nitrogen metabolism, and can synthesize the synthesis that L-glutamic acid is γ-PGA in microorganism cells provides a large amount of precursors.
(5) culture in the present invention after fermentation contains γ-PGA and subtilis Bacillus subtilis, can directly be applied in agriculture production as organic fertilizer, turn, this reduces the technique extracted needed for γ-PGA, the exploitation for agriculture level γ-PGA provides new thinking and prospect widely.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, the content described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
It is B.subtilis PG-8 that γ-PGA used in following examples produces bacterium, and this bacterial strain has been deposited in China General Microbiological preservation center, and deposit number is CGMCC NO.6324.
Needle mushroom dreg solid fermentation is utilized to produce γ-PGA in embodiment 1:500mL triangular flask.
(1), B.subtilis PG-8 solid fungicide preparation: produce after γ-PGA terminate 10 tons of fermentor tank top fermentations, collect the microbial cells in fermented liquid, and add carrier according to mass ratio 1.5:2.4, mix to be placed in revolution vacuum double-cone dryer to dry at 50 DEG C to water content and be about 8%, finally prevent the moisture absorption with packaging of aluminium foil bag, can obtain B.subtilis PG-8 solid fungicide, contained viable count is 5.0 × 10
10cfu/g.
(2), solid fermentation: solid fermentation nutrient media components (solid substrate 20g) is needle mushroom dreg 10g, msg meal 10g, inulin 5g, corn cob meal 2.4g, MnSO
4h
2o 0.002g, MgSO
47H
2o 0.05g; Substratum initial water is divided into 60%, pH to be 7.0; 121 DEG C of sterilizing 15min.The B.subtilisPG-8 solid fungicide 0.5g taking preparation in step (1) is inoculated in sterilized fermention medium, after mixing, be placed in shaking table tranquillization and cultivate 48h, leavening temperature is set to 30 DEG C, stirs for several times in fermenting process every 8h in super clean bench with sterile glass rod.
(3), the extraction of γ-PGA and detection: get the culture 5g after fermentation, 1.5h is stirred under the distilled water (v/w) adding 10 times of volumes and culture mix the shaking table being incorporated in 150rpm, then by mixture centrifugal 5min under 5000rpm, get supernatant liquor and dilute 15 times, HPLC detection is carried out after removing thalline with 0.22 μm of filter membrane, upper sample is 20 μ L, and chromatographic column is Shodex Ohpak SB-806M HQ, and moving phase is 0.2M Na
2sO
4solution, pH 4.0 adjusted by acetic acid, and flow velocity is 1.0mL/min.γ-PGA the concentration (g/L) that HPLC measures is replaced as g/kg solid medium.
Adopt needle mushroom dreg solid fermentation in 500mL triangular flask can produce 146g/kg γ-PGA.
Flat mushroom bacterium slag solid fermentation is utilized to produce γ-PGA in embodiment 2:500mL triangular flask.
(1), in this embodiment about B.subtilis PG-8 solid fungicide preparation and the extracting method of γ-PGA, identical with working method in embodiment 1.
(2), solid fermentation: fermention medium component (solid substrate 20g) is flat mushroom bacterium slag 13g, msg meal 7g, inulin 7.5g, corn cob meal 1.6g, MnSO
4h
2o 0.004g, MgSO
47H
2o 0.05g; Substratum initial water is divided into 65%, pH to be 7.0; 121 DEG C of sterilizing 15min.The B.subtilis PG-8 solid fungicide 0.8g taking preparation in step (1) is inoculated in sterilized fermention medium, after mixing, be placed in shaking table tranquillization and cultivate 48h, leavening temperature is set to 32 DEG C, stirs for several times in fermenting process every 8h in super clean bench with sterile glass rod.
(3), the extraction of γ-PGA and detection: extract the γ-PGA supernatant liquor liquid obtained and dilute 20 times, carry out HPLC detection, testing conditions is with described in embodiment 1.
Adopt flat mushroom bacterium slag to produce γ-PGA concentration at 500mL triangular flask solid fermentation and can reach 153g/kg.
Lentinus Edodes fungus slag solid fermentation is utilized to produce γ-PGA in embodiment 3:500mL triangular flask.
(1), in this embodiment about B.subtilis PG-8 solid fungicide preparation and the extracting method of γ-PGA, identical with working method in embodiment 1.
(2), solid fermentation: fermention medium component (solid substrate 20g) is Lentinus Edodes fungus slag 15g, msg meal 5g, inulin 3g, corn cob meal 2g, MnSO
4h
2o 0.001g, MgSO
47H
2o 0.03g substratum initial water is divided into 70%, pH to be 7.0; 121 DEG C of sterilizing 15min.The B.subtilis PG-8 solid fungicide 1g taking preparation in step (1) is inoculated in sterilized fermention medium, after mixing, be placed in shaking table tranquillization and cultivate 56h, leavening temperature is set to 35 DEG C, stirs for several times in fermenting process every 8h in super clean bench with sterile glass rod.
(3), the extraction of γ-PGA and detection: extract the γ-PGA supernatant liquor liquid obtained and dilute 25 times, carry out HPLC detection, testing conditions is with described in embodiment 1.
Adopt Lentinus Edodes fungus slag to produce γ-PGA concentration at 500mL triangular flask solid fermentation and can reach 164g/kg.
Mixed bacterium slag solid fermentation is utilized to produce γ-PGA in embodiment 4:500mL triangular flask.
(1), in this embodiment about B.subtilis PG-8 solid fungicide preparation and the extracting method of γ-PGA, identical with working method in embodiment 1.
(2), solid fermentation: fermention medium component (solid substrate 20g) is flat mushroom bacterium slag 6g, Lentinus Edodes fungus slag 6g, msg meal 8g, inulin 4g, corn cob meal 2.5g, MnSO
4h
2o 0.003g, MgSO
47H
2o 0.03g substratum initial water is divided into 70%, pH to be 7.0; 121 DEG C of sterilizing 15min.The B.subtilisPG-8 solid fungicide 1g taking preparation in step (1) is inoculated in sterilized fermention medium, after mixing, be placed in shaking table tranquillization and cultivate 48h, leavening temperature is set to 35 DEG C, stirs for several times in fermenting process every 8h in super clean bench with sterile glass rod.
(3), the extraction of γ-PGA and detection: extract the γ-PGA supernatant liquor liquid obtained and dilute 25 times, carry out HPLC detection, testing conditions is with described in embodiment 1.
Adopt mixed bacterium slag to produce γ-PGA concentration at 500mL triangular flask solid fermentation and can reach 150g/kg.
Mixed bacterium slag solid fermentation is utilized to produce γ-PGA in embodiment 5:500mL triangular flask.
(1), in this embodiment about B.subtilis PG-8 solid fungicide preparation and the extracting method of γ-PGA, identical with working method in embodiment 1.
(2), solid fermentation: fermention medium component (solid substrate 20g) is flat mushroom bacterium slag 3g, needle mushroom dreg 5g, Lentinus Edodes fungus slag 6g, msg meal 6g, inulin 6g, corn cob meal 2.5g, MnSO
4h
2o 0.004g, MgSO
47H
2o0.05g; Substratum initial water is divided into 70%, pH to be 7.0; 121 DEG C of sterilizing 15min.The B.subtilis PG-8 solid fungicide 1g taking preparation in step (1) is inoculated in sterilized fermention medium, after mixing, be placed in shaking table tranquillization and cultivate 56h, leavening temperature is set to 35 DEG C, stirs for several times in fermenting process every 8h in super clean bench with sterile glass rod.
(3), the extraction of γ-PGA and detection: extract the γ-PGA supernatant liquor liquid obtained and dilute 25 times, carry out HPLC detection, testing conditions is with described in embodiment 1.
Adopt mixed bacterium slag to produce γ-PGA concentration at 500mL triangular flask solid fermentation and can reach 175g/kg.
Mixed bacterium slag solid fermentation is utilized to produce γ-PGA in embodiment 6:5L filter flask.
(1), in this embodiment about B.subtilis PG-8 solid fungicide preparation and the extracting method of γ-PGA, identical with working method in embodiment 1.
(2), solid fermentation: fermention medium component (solid substrate 200g) is needle mushroom dreg 55g, Lentinus Edodes fungus slag 85g, msg meal 60g, inulin 65g, corn cob meal 25g, MnSO
4h
2o 0.03g, MgSO
47H
2o 0.6g; Substratum initial water is divided into 65%, pH to be 7.0; 121 DEG C of sterilizing 15min.The B.subtilis PG-8 solid fungicide 8g taking preparation in step (1) is inoculated in sterilized fermention medium, after mixing, be placed in shaking table tranquillization and cultivate 56h, leavening temperature is set to 35 DEG C, stirs for several times in fermenting process every 8h in super clean bench with sterile glass rod.
(3), the extraction of γ-PGA and detection: extract the γ-PGA supernatant liquor liquid obtained and dilute 25 times, carry out HPLC detection, testing conditions is with described in embodiment 1.
Adopt mixed bacterium slag solid fermentation in 5L filter flask to produce γ-PGA concentration and can reach 162g/kg.
Mixed bacterium slag solid fermentation is utilized to produce γ-PGA in embodiment 7:5L filter flask.
(1), in this embodiment about B.subtilis PG-8 solid fungicide preparation and the extracting method of γ-PGA, identical with working method in embodiment 1.
(2), solid fermentation: fermention medium component (solid substrate 200g) is flat mushroom bacterium slag 35g, needle mushroom dreg 60g, Lentinus Edodes fungus slag 60g, msg meal 45g, inulin 50g, corn cob meal 20g, MnSO
4h
2o 0.04g, MgSO
47H
2o 0.6g; Substratum initial water is divided into 65%, pH to be 7.0; 121 DEG C of sterilizing 15min.The B.subtilis PG-8 solid fungicide 8g taking preparation in step (1) is inoculated in sterilized fermention medium, after mixing, be placed in shaking table tranquillization and cultivate 56h, leavening temperature is set to 35 DEG C, stirs for several times in fermenting process every 8h in super clean bench with sterile glass rod.
(3), the extraction of γ-PGA and detection: extract the γ-PGA supernatant liquor liquid obtained and dilute 25 times, carry out HPLC detection, testing conditions is with described in embodiment 1.
Adopt mixed bacterium slag solid fermentation in 5L filter flask to produce γ-PGA concentration and can reach 168g/kg.
Embodiment 8:3m
2field composted solids fermentative production γ-PGA.
This embodiment selects well-ventilated, the outdoor of spaciousness carries out, and compost experimental size is length 2m, width 1.5m, total area 3m
2, culture ulking thickness is approximately 5cm.
(1), in this embodiment about B.subtilis PG-8 solid fungicide preparation and the extracting method of γ-PGA, identical with working method in embodiment 1.
(2), solid fermentation: fermention medium component (solid substrate 15kg) is flat mushroom bacterium slag 3.5kg, needle mushroom dreg 4kg, Lentinus Edodes fungus slag 4kg, msg meal 3.5kg, inulin 3.5kg, corn cob meal 1.5kg, MnSO
4h
2o1g, MgSO
47H
2o 30g; Substratum initial water is divided into 65%, pH to be 7.0; The B.subtilis PG-8 solid fungicide 0.5kg taking preparation in step (1) is inoculated in fermention medium, and after mixing, be placed in shaking table tranquillization and cultivate 48h, leavening temperature is 20-40 DEG C, in fermenting process every 8h in upset for several times.
(3), the extraction of γ-PGA and detection: extract the γ-PGA supernatant liquor liquid obtained and dilute 25 times, carry out HPLC detection, testing conditions is with described in embodiment 1.
Employing mixed bacterium slag in the wild composted solids fermentative production γ-PGA concentration can reach 160g/kg.
Claims (10)
1. utilize edible fungi residue solid fermentation to produce a method for gamma-polyglutamic acid-, it is characterized in that, the method comprises the steps:
(1) preparation of solid fungicide: gamma-polyglutamic acid-is produced the fermented liquid that bacterium obtains after liquid fermenting and process, collect thalline, mixed by thalline with carrier, drying, obtains solid fungicide;
(2) solid fermentation: the solid fungicide that step (1) obtains is inoculated in solid fermentation substratum, mixes, proceed solid fermentation and prepare gamma-polyglutamic acid-;
Described solid fermentation substratum is made up of solid substrate and additives, and described solid substrate is the mixture of edible fungi residue and msg meal.
2. the method utilizing edible fungi residue solid fermentation to produce gamma-polyglutamic acid-according to claim 1, it is characterized in that, in step (1), it is B.subtilis PG-8 that described gamma-polyglutamic acid-produces bacterium, and the deposit number of this bacterial strain is CGMCC NO.6324.
3. the method utilizing edible fungi residue solid fermentation to produce gamma-polyglutamic acid-according to claim 1, it is characterized in that, in step (1), described carrier is the mixture of wheat bran and bagasse, wherein, the mass ratio of wheat bran and bagasse is 0.7 ~ 1.2:1 ~ 1.5.
4. the method utilizing edible fungi residue solid fermentation to produce gamma-polyglutamic acid-according to claim 1, it is characterized in that, in step (1), it is 0.8 ~ 2.4:1.5 ~ 3 that thalline and carrier add mass ratio.
5. the method utilizing edible fungi residue solid fermentation to produce gamma-polyglutamic acid-according to claim 1, is characterized in that, in step (1), containing viable count in described solid fungicide is 5 ~ 6.5 × 10
10cfu/g, described solid fungicide water content is lower than 10%.
6. the method utilizing edible fungi residue solid fermentation to produce gamma-polyglutamic acid-according to claim 1, it is characterized in that, in step (2), described solid fermentation, its leavening temperature is 30 ~ 35 DEG C, and fermentation time is 48 ~ 56h.
7. the method utilizing edible fungi residue solid fermentation to produce gamma-polyglutamic acid-according to claim 1, it is characterized in that, in step (2), in described solid substrate, the mass ratio of edible fungi residue and msg meal is 1 ~ 1.6:0.5 ~ 1.
8. the edible fungi residue solid fermentation that utilizes according to claim 1 or 7 produces the method for gamma-polyglutamic acid-, and it is characterized in that, described edible fungi residue is any one or a few the mixture in needle mushroom dreg, flat mushroom bacterium slag and Lentinus Edodes fungus slag.
9. the method utilizing edible fungi residue solid fermentation to produce gamma-polyglutamic acid-according to claim 1, it is characterized in that, in step (2), in solid fermentation substratum, additives and content thereof are: inulin 150 ~ 400g/kg, corn cob meal 50 ~ 200g/kg, MnSO
4h
2o 50 ~ 200mg/kg, MgSO
47H
2o0.5 ~ 3g/kg.
10. the method utilizing edible fungi residue solid fermentation to produce gamma-polyglutamic acid-according to claim 1, it is characterized in that, in step (2), the pH of described solid fermentation substratum is 7.0, and the humidity of water use regulation solid medium is more than 50%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510081839.7A CN104673851B (en) | 2015-02-15 | 2015-02-15 | A kind of method using edible fungi residue solid fermentation production gamma-polyglutamic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510081839.7A CN104673851B (en) | 2015-02-15 | 2015-02-15 | A kind of method using edible fungi residue solid fermentation production gamma-polyglutamic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104673851A true CN104673851A (en) | 2015-06-03 |
| CN104673851B CN104673851B (en) | 2018-06-08 |
Family
ID=53309439
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510081839.7A Active CN104673851B (en) | 2015-02-15 | 2015-02-15 | A kind of method using edible fungi residue solid fermentation production gamma-polyglutamic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104673851B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105130685A (en) * | 2015-09-30 | 2015-12-09 | 富朗（中国）生物科技有限公司 | Preparation method for organic fertilizer containing Nu-polyglutamic acid |
| CN105198512A (en) * | 2015-10-26 | 2015-12-30 | 富朗（中国）生物科技有限公司 | Method for preparing gamma-polyglutamic acid organic fertilizer by double microbial fermentation |
| CN107236685A (en) * | 2017-05-22 | 2017-10-10 | 中国科学院成都生物研究所 | A kind of method that utilization sludge produces γ polyglutamic acid organic fertilizers |
| CN107365808A (en) * | 2017-07-31 | 2017-11-21 | 常州市天宁区鑫发织造有限公司 | A kind of preparation method for being used to produce γ polyglutamic acid biologic grains |
| CN107596429A (en) * | 2017-09-29 | 2018-01-19 | 南京轩凯生物科技有限公司 | A kind of blood protein response type γ polyglutamic acid hydrogel hemostatic materials and its preparation method and application |
| CN107937449A (en) * | 2016-10-12 | 2018-04-20 | 镇江市天益生物科技有限公司 | With the method for needle mushroom waste material production γ polyglutamic acids |
| CN107937450A (en) * | 2016-10-12 | 2018-04-20 | 镇江市天益生物科技有限公司 | With the method for Pleurotus eryngii waste material production γ polyglutamic acids |
| CN107937451A (en) * | 2016-10-12 | 2018-04-20 | 镇江市天益生物科技有限公司 | With the method for mushroom waste material production γ polyglutamic acids |
| CN113584097A (en) * | 2021-06-28 | 2021-11-02 | 杨凌未来中科环保科技有限公司 | Fermentation method and device for producing gamma-polyglutamic acid by reflux type solid fermentation |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101417894A (en) * | 2008-04-09 | 2009-04-29 | 广东省微生物研究所 | Method for producing fertilizer additive agent containing gamma-polyglutamic acid |
| CN101705260A (en) * | 2009-11-16 | 2010-05-12 | 天津北洋百川生物技术有限公司 | Method for producing gamma-polyglutamic acid by inert carrier solid state fermentation method |
| CN102965311A (en) * | 2012-11-16 | 2013-03-13 | 南京轩凯生物科技有限公司 | Bacillus subtilis and application thereof in preparation of gamma-D-polyglutamic acid |
| CN103444981A (en) * | 2012-05-29 | 2013-12-18 | 江苏大学 | Method for Aspergillus oryzae to degrade edible and medicinal fungus dregs to produce protein feed |
| CN103468596A (en) * | 2012-06-08 | 2013-12-25 | 连云港荣盛生物科技有限公司 | Method for utilizing waste edible fungi bags to ferment facultative anaerobic bacillaceae |
-
2015
- 2015-02-15 CN CN201510081839.7A patent/CN104673851B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101417894A (en) * | 2008-04-09 | 2009-04-29 | 广东省微生物研究所 | Method for producing fertilizer additive agent containing gamma-polyglutamic acid |
| CN101705260A (en) * | 2009-11-16 | 2010-05-12 | 天津北洋百川生物技术有限公司 | Method for producing gamma-polyglutamic acid by inert carrier solid state fermentation method |
| CN103444981A (en) * | 2012-05-29 | 2013-12-18 | 江苏大学 | Method for Aspergillus oryzae to degrade edible and medicinal fungus dregs to produce protein feed |
| CN103468596A (en) * | 2012-06-08 | 2013-12-25 | 连云港荣盛生物科技有限公司 | Method for utilizing waste edible fungi bags to ferment facultative anaerobic bacillaceae |
| CN102965311A (en) * | 2012-11-16 | 2013-03-13 | 南京轩凯生物科技有限公司 | Bacillus subtilis and application thereof in preparation of gamma-D-polyglutamic acid |
Non-Patent Citations (1)
| Title |
|---|
| 张丽美等: "枯草芽孢杆菌发酵菌糠制备饲料微生物添加剂的研 枯草芽孢杆菌发酵菌糠制备饲料微生物添加剂的研究", 《饲料工业》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105130685A (en) * | 2015-09-30 | 2015-12-09 | 富朗（中国）生物科技有限公司 | Preparation method for organic fertilizer containing Nu-polyglutamic acid |
| CN105198512A (en) * | 2015-10-26 | 2015-12-30 | 富朗（中国）生物科技有限公司 | Method for preparing gamma-polyglutamic acid organic fertilizer by double microbial fermentation |
| CN105198512B (en) * | 2015-10-26 | 2018-09-07 | 富朗（中国）生物科技有限公司 | A kind of method that double-microbial fermentation prepares gamma-polyglutamic acid organic fertilizer |
| CN107937450A (en) * | 2016-10-12 | 2018-04-20 | 镇江市天益生物科技有限公司 | With the method for Pleurotus eryngii waste material production γ polyglutamic acids |
| CN107937451A (en) * | 2016-10-12 | 2018-04-20 | 镇江市天益生物科技有限公司 | With the method for mushroom waste material production γ polyglutamic acids |
| CN107937449A (en) * | 2016-10-12 | 2018-04-20 | 镇江市天益生物科技有限公司 | With the method for needle mushroom waste material production γ polyglutamic acids |
| CN107236685B (en) * | 2017-05-22 | 2020-06-02 | 中国科学院成都生物研究所 | Method for producing gamma-polyglutamic acid organic fertilizer by using sludge |
| CN107236685A (en) * | 2017-05-22 | 2017-10-10 | 中国科学院成都生物研究所 | A kind of method that utilization sludge produces γ polyglutamic acid organic fertilizers |
| CN107365808A (en) * | 2017-07-31 | 2017-11-21 | 常州市天宁区鑫发织造有限公司 | A kind of preparation method for being used to produce γ polyglutamic acid biologic grains |
| CN107596429A (en) * | 2017-09-29 | 2018-01-19 | 南京轩凯生物科技有限公司 | A kind of blood protein response type γ polyglutamic acid hydrogel hemostatic materials and its preparation method and application |
| CN107596429B (en) * | 2017-09-29 | 2020-09-29 | 南京轩凯生物科技有限公司 | Blood protein response type gamma-polyglutamic acid hydrogel hemostatic material and preparation method and application thereof |
| CN113584097A (en) * | 2021-06-28 | 2021-11-02 | 杨凌未来中科环保科技有限公司 | Fermentation method and device for producing gamma-polyglutamic acid by reflux type solid fermentation |
| CN113584097B (en) * | 2021-06-28 | 2023-08-18 | 杨凌未来中科环保科技有限公司 | Fermentation method and device for producing gamma-polyglutamic acid through reflux type solid fermentation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104673851B (en) | 2018-06-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104673851A (en) | Method for preparing gamma-polyglutamic acid through soild fermentation by utilizing edible fungi residues | |
| CN106396817B (en) | Method for preparing organic fertilizer based on straw and livestock and poultry manure | |
| CN101244955B (en) | Biological fertilizer and manufacture method thereof | |
| Moshi et al. | Characterisation and evaluation of a novel feedstock, Manihot glaziovii, Muell. Arg, for production of bioenergy carriers: Bioethanol and biogas | |
| CN105130685A (en) | Preparation method for organic fertilizer containing Nu-polyglutamic acid | |
| CN111073839B (en) | Siam bacillus, microbial inoculum and application thereof | |
| CN109486684B (en) | Low-temperature straw degradation fungus JGDW-1 and microbial inoculum and application thereof | |
| CN109536410B (en) | Salt-tolerant growth-promoting composite microbial inoculum and preparation method and application thereof | |
| CN105647832A (en) | High-temperature-resistant garden waste decomposition bacterium FHM1 and application thereof | |
| CN114561327B (en) | Cellulose degradation composite microbial inoculant, and preparation method and application thereof | |
| CN115536445A (en) | Method for preparing liquid organic base fertilizer from kitchen waste, liquid organic base fertilizer and liquid organic fertilizer | |
| CN103966265A (en) | Method for preparing biogas by pretreating manioc waste with NaOH through anaerobic fermentation | |
| CN103352016B (en) | Method for preparing biological fertilizer by utilizing Alteromonas colwelliana A321 to ferment enteromorpha | |
| CN105969697A (en) | Compound bacterium agent for composting straw as well as preparation method and application thereof | |
| CN110396483B (en) | High-temperature straw degradation bacterium B-8, and microbial inoculum and application thereof | |
| CN111154661B (en) | Complex microbial inoculant and application thereof | |
| CN102978142B (en) | Rice endophyte (Pantoea sp. Sd-1) for efficiently degrading lignin | |
| CN110845253B (en) | Preparation and application method of gamma-polyglutamic acid biological organic fertilizer | |
| CN112226390A (en) | Compound functional microbial inoculum for composting fermentation and kitchen waste composting method | |
| CN114456980B (en) | Gamma-polyglutamic acid high-yield strain and application thereof | |
| CN102732463B (en) | Bacillus subtilis RB and application thereof | |
| CN114075520B (en) | Bacillus belicus for producing gamma-polyglutamic acid and method for producing gamma-polyglutamic acid by solid fermentation of bacillus belicus | |
| CN114015619A (en) | Straw fermentation composite bacterium preparation and preparation method thereof | |
| CN105907685A (en) | Preparing method for complex microorganism bacterium agent | |
| CN101307331A (en) | Process for producing gardening base material by mixing fermentation of corn stalk powder and peanut shell powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201126 Address after: 261000 north of Shenghai East Road and west of Chuang Road, Yangkou Town, Shouguang City, Weifang City, Shandong Province Patentee after: Xuankai Biotechnology (Shandong) Co.,Ltd. Address before: 509-5 room 15, building 210029, hi tech Development Zone, Jiangsu, Nanjing Patentee before: NANJING SHINEKING BIOTECH Co.,Ltd. Patentee before: NANJING JUTAI HI-TECH AGRICULTURE Co.,Ltd. |