CN104862260B - One plant of arthrobacterium and its application with aerobic denitrification ability - Google Patents
One plant of arthrobacterium and its application with aerobic denitrification ability Download PDFInfo
- Publication number
- CN104862260B CN104862260B CN201510315944.2A CN201510315944A CN104862260B CN 104862260 B CN104862260 B CN 104862260B CN 201510315944 A CN201510315944 A CN 201510315944A CN 104862260 B CN104862260 B CN 104862260B
- Authority
- CN
- China
- Prior art keywords
- arthrobacterium
- arthrobacter
- dys3
- nitrite
- denitrification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/06—Arthrobacter
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
Abstract
One plant of arthrobacterium and its application with aerobic denitrification ability, belong to technical field of microbe application.One plant of arthrobacterium (Arthrobacter sp.) DYS3 4 with aerobic denitrification ability, is preserved in China typical culture collection center, and the deposit date is on 04 29th, 2015, preserving number was CCTCC M 2015264.Arthrobacterium (Arthrobacter sp.) DYS3 4 can be applied in nitrogenous effluent biological denitrificaion, in the removal of sea-farming nitrite, in the removal of freshwater aquiculture nitrite and in preparing low-temperature denitrification microecological microbial agent.Under aerobic condition, the NO3-N and NO2-N in water body environment can be rapidly and efficiently removed.The strain growth temperature range is wider, and preferable denitrification effect is all had at 10~35 DEG C, has preferable application prospect in nitrogenous effluent biological denitrificaion and sea water and fresh water cultivation nitrite removal.
Description
Technical field
The invention belongs to technical field of microbe application, and in particular to one plant of arthrobacterium with aerobic denitrification ability
(Arthrobacter sp.) DYS3-4 and its application.
Background technology
In natural water body, nitrogen exists in the form of -3 to+5 nine kinds of different valence states, in biology and the common work of abiotic factor
Under, they move about conversion in water body, constitute a complicated dynamic circulation.Wherein ammonia nitrogen, nitrite etc. can
To lead to the acidification to lake, river, coastal ocean etc. and eutrophication, while as the inorganic nitrification with genotoxic potential
Object also has toxic action to aquaculture organism.How nitrate pollution in water body is economically and efficiently removed, it has also become water pollution
The primary study content of control field.
Include at present mainly Physical, chemical method and biological denitrificaion method applied to the method for water body denitrification.Wherein, physics,
Chemical method is complicated for operation, needs to increase equipment investment volume, operating cost is high.This method is low to organic matter removal efficiency simultaneously, meeting
Secondary pollution is caused, therefore is had some limitations in practice.In recent years, people begin attempt to using microorganism,
Pollutant existing forms are eliminated or changed to the function that plant or other biological itself have, and reaches the mesh for reducing pollutant toxicity
's.Compared with physics, chemical denitrogenation, biological denitrificaion only leans on the energy circulation of biology itself and substance cycle to act as in system
With not introducing other foreign substances.Since bio-denitrification technology is thorough etc. at low cost, easy to operate, non-secondary pollution, denitrogenation
Advantage is gradually applied to industrial wastewater denitrogenation processing.
The microorganism for participating in biological denitrificaion includes mainly nitrobacteria and denitrifying bacteria.Traditional theory thinks, bacterium
Denitrification is a stringent anaerobic processes, and denitrifying bacteria can be preferentially using oxygen as final electron acceptor.It is aerobic
The discovery of denitrifying bacteria, breaches traditional understanding, at the same also for people study new bio-denitrification technology provide it is new
Thinking.Aerobic denitrification, referring to can be with NO under aerobic conditions3 --N、NO2 -- N carries out denitrification mistake as final electron acceptor
Journey.The aerobic denitrifying bacteria having now been found that is concentrated mainly on pseudomonas (Pseudomonas), alcaligenes
(Alcaligenes), paracoccus (Paracoccus) and bacillus (Bacillus).Although both at home and abroad for aerobic anti-
The separation of nitrifier, screening and mechanism study are more, but excellent species and its application are less, and it is raw still to cannot be satisfied industry at present
The demand of production.Separation screening high-efficiency aerobic denitrifying bacteria, and its aerobic denitrification characteristic is studied, to nitrogenous effluent
Biological denitrificaion has important theory value and practical significance.
Chinese patent CN 201010536224.6 discloses one plant of nitrite type denitrification bacterial strain and its application.The invention
The bacterial strain of short-cut denitrification denitrogenation is arthrobacterium (Arthrobacter creatinolyticus) FDN-1, on March 11st, 2010
It is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms center ", preserving number is CGMCC NO.3657.It should
Bacterial strain can directly adopt nitrite nitrogen as substrate, complete short-cut denitrification process.The arthrobacterium FDN-1 of the invention is locating
It is simple for process when managing ammonia-containing water, still there is higher denitrification activity in the presence of low concentration organic carbon source, simultaneously
It is resistant to high concentration organic carbon source.System starts fast after coming into operation, and has very in all kinds of denitrogenation of waste water processing procedures
Wide application prospect.
Chinese patent CN 201010536004.3 discloses a kind of microbial bacteria carrying out denitrification denitrogenation using nitrite
Agent, it is characterised in that：Microbial bacterial agent includes arthrobacterium (Arthrobacter creatinolyticus) FDN-1 and Shui Shi yellow
Bacillus (Flavobacterium mizutaii) FDN-2；Arthrobacterium FDN-1 and Shui Shi Flavobacterium FDN-2 preservation registration numbers are distinguished
For CGMCC No.3657 and CGMCC No.3659.The microbial bacterial agent can carry out denitrification using nitrite as electron acceptor
Denitrogenation can also effectively remove the COD in waste water while denitrogenation, and good waste water treatment effect, denitrification rates are fast, can stablize control
The process of short-cut denitrification processed really realizes short-cut nitrification and denitrification denitrogenation.
Chinese patent CN 201410335917.7 discloses one plant of nicotianae bacterial strain YY-9 and its in aquaculture
Using.It is related to one plant of nicotianae YY-9, CCTCC NO:M2014278, and its application in handling breeding wastewater ammonia nitrogen.
The bacterium has very strong degradation capability to the ammoniacal nitrogen in breeding wastewater, and the removal rate of ammoniacal nitrogen is up to after 48h processing
92.70%, purifying water effect is notable；In addition, the water body in hut shrimp aquaculture pond is directly handled using the bacterial strain, NH after 48h4 +- N's
Removal rate is up to 90.28%.Bacterial strain YY-9 can effectively solve the problems, such as that aquaculture ammoniacal nitrogen is excessively high, mitigate high-density breeding pair
The pollution of water body.
Invention content
The purpose of the present invention is to provide one plant of arthrobacteriums (Arthrobacter sp.) with aerobic denitrification ability
DYS3-4。
Another object of the present invention is to provide arthrobacterium (Arthrobacter sp.) DYS3-4 in nitrogenous effluent biology
Denitrogenation and the application in sea water and fresh water cultivation nitrite removal.
Arthrobacterium (Arthrobacter sp.) DYS3-4 has been deposited in Chinese Typical Representative training on 04 29th, 2015
Object collection is supported, address is Wuhan, China Wuhan University, and deposit number is CCTCC NO:M 2015264.
Arthrobacterium (Arthrobacter sp.) DYS3-4 is from Penaeus Vannmei seawater-culture pond orienting enriching point
From acquisition.
Arthrobacterium (Arthrobacter sp.) DYS3-4 is also deposited in Chinese Sea Microbiological Culture Collection management
Center (MCCC), deposit number are MCCC 1A10907.
Arthrobacterium (Arthrobacter sp.) DYS3-4 has following characteristics：
1, morphological feature：Arthrobacterium (Arthrobacter sp.) DYS3-4, Gram-negative are solid in Marine Agar
For 24 hours, for bacterium colony in light yellow, surface is smooth, microprotrusion, regular edges, opaque, colony diameter is big for 28 DEG C of cultures on body culture medium
Small about 0.5~1mm.
2, physiological and biochemical property：5~40 DEG C, optimum growth temperature is 25~35 DEG C；It is 5.5~9.5 to grow pH, most adaptability
Long pH is 8.5；The NaCl concentration of growth is 0~10%, and the NaCl concentration of the most suitable growth is 3%；The carbon source of the most suitable growth is second
Sour sodium.Oxidizing ferment (-), catalase (+), nitrate reduction (+), indole test (-), D-Glucose fermentation and acid (-), arginine
Double hydrolases (+), gelatin liquefaction (+), urease (+), beta-glucosidase (W) can utilize D-Glucose, PEARLITOL 25C, wheat
Bud sugar, gluconate, malic acid, citric acid, phenylacetic acid, L-arabinose, cannot utilize capric acid, adipic acid, D-MANNOSE,
N- acetyl-gucosamine, beta galactosidase.
API ZYM are the results show that bacterial strain DYS3-4 has alkaline phosphatase, esterase, leucine amino peptidase, valine ammonia peptide
Enzyme, cystine aminopeptidase, trypsase, acid phosphatase, α-Portugal (grape) glycosidase do not have, β-Portugal (grape) glycosidase, α-half
Lactoside enzyme, beta galactosidase, alpha -chymotrypsin, β-Portugal (grape) glycuronides enzyme, N- acetyl-glucosaminidase, α-are sweet
Reveal the enzymatic activitys such as glycosidase, Alpha-Fucosidase.
3,16S rRNA and gyrB gene sequencings：Conventionally extracting arthrobacterium (Arthrobacter sp.)
The genomic DNA of DYS3-4, and 16S rRNA gene orders are measured, arthrobacterium (Arthrobacter sp.) DYS3-4
16S rRNA gene orders as shown in SEQ ID NO.1.
Through BLAST compare analyze, the 16S rRNA genes of arthrobacterium (Arthrobacter sp.) DYS3-4 with
Arthrobacter nicotianae DSM 20123 (T) X80739 similitude highests, respectively 99.14%, be secondly
A.arilaitensis Re117 (T) FQ311875 and A.soli SYB2 (T) EF660748, respectively 98.93% He
98.21%.
Morphological feature, physio-biochemical characteristics and the 16S rDNA gene sequencings of comprehensive bacterial strain are as a result, the present invention
Arthrobacterium (Arthrobacter sp.) DYS3-4 belongs to Arthrobacter, specially arthrobacterium (Arthrobacter sp.)
DYS3-4。
The bacterial strain can rapidly and efficiently remove nitrate, nitrite and the ammonium salt in water body environment under aerobic condition, and
There is stronger tolerance and removal ability to nitrite.The bacterium growth temperature range is wide, can grow and take off at 5~40 DEG C
Nitrogen.
It can be seen that arthrobacterium (Arthrobacter sp.) DYS3-4 can be in nitrogenous effluent biological denitrificaion, sea-farming
Nitrite removal, the removal of freshwater aquiculture nitrite are applied in preparing low-temperature denitrification microecological microbial agent etc.
The nitrogenous effluent includes but not limited to nitrate wastewater, nitrite waste water, ammonia nitrogen waste water etc..
The positive effect of the present invention is：A kind of aerobic denitrifying bacteria is provided, the bacterial strain is equal under aerobic and oxygen free condition
Using nitrate or nitrite efficient denitrification can be carried out as nitrogen source；The wide temperature range of suitable growth, at 5~40 DEG C
It can grow and denitrogenation, and the nitric efficiency highest at 15~35 DEG C；There is stronger tolerance and removal energy to nitrite
Power.
Description of the drawings
The nitric efficiency of arthrobacterium (Arthrobacter sp.) DYS3-4 when Fig. 1 is 10 DEG C.
The nitric efficiency of arthrobacterium (Arthrobacter sp.) DYS3-4 when Fig. 2 is 15 DEG C.
The nitric efficiency of arthrobacterium (Arthrobacter sp.) DYS3-4 when Fig. 3 is 25 DEG C.
The nitric efficiency of arthrobacterium (Arthrobacter sp.) DYS3-4 when Fig. 4 is 28 DEG C.
The nitric efficiency of arthrobacterium (Arthrobacter sp.) DYS3-4 when Fig. 5 is 35 DEG C.
Specific implementation mode
The present invention will be further described below with reference to the drawings, but does not therefore limit the present invention to the implementation
Among example range.Method in following embodiments is unless otherwise instructed conventional method.
Enrichment, separation and the identification of 1 arthrobacterium of embodiment (Arthrobacter sp.) DYS3-4
1, the enrichment and separation of bacterial strain：ZhangZhou Penaeus Vannmei seawater-culture pond sewage sample 10mL is taken, it is rich that 90mL is added
Collect culture medium (sodium acetate 1g/L, sodium citrate 2.5g/L, NaCl 30g/L, KH2PO42g/L, Na2HPO43g/L, MgSO4·
7H2O 0.05g/L、FeSO4·7H2O 0.02g/L、CaCl27.6~8.2,121 DEG C of 0.02g/L, distilled water 1000mL, pH goes out
Bacterium 20min), with 0.5g/L NO2 -- N is used as only nitrogen source, and 15 DEG C, it is thin to carry out aerobic denitrification for 160rpm/min shake cultures
The first time of bacterium is enriched with.After 15 days, 10mL sample liquids are taken from first enrichment culture liquid, are transferred in fresh enrichment culture liquid,
With 1.0g/L NO2 -- N is used as only nitrogen source, and 15 DEG C, 160rpm/min shake cultures carry out second and are enriched with.After 15d, from two
10mL sample liquids are taken in secondary enrichment culture liquid, are transferred in fresh enrichment culture liquid, addition 1.5g/L NO2 -- N is as unique
Nitrogen source, 15 DEG C, 160rpm/min shake cultures carry out third time enrichment.After completing enrichment culture three times, applied using gradient dilution
Cloth flat band method is isolated and purified to obtain arthrobacterium (Arthrobacter sp.) respectively with Marine Agar solid medium coated plates
The single bacterium of DYS3-4.
2, the physiological and biochemical property and Molecular Identification of bacterial strain
Morphological feature：Arthrobacterium (Arthrobacter sp.) DYS3-4, Gram-negative, in Marine Agar solids
On culture medium 28 DEG C culture for 24 hours, bacterium colony in it is light yellow, surface is smooth, microprotrusion, regular edges, opaque, colony diameter size
About 0.5~1mm.
Physiological and biochemical property：
5~40 DEG C, optimum growth temperature is 25~35 DEG C；It is 5.5~9.5 to grow pH, and the most suitable growth pH is 8.5；Growth
NaCl concentration be 0~10%, the NaCl concentration of the most suitable growth is 3%；The carbon source of the most suitable growth is sodium acetate.Oxidizing ferment (-),
Catalase (+), nitrate reduction (+), indole test (-), D-Glucose fermentation and acid (-), arginine dihydrolase (+) are bright
Dispergation (+), urease (+), beta-glucosidase (W) can utilize D-Glucose, PEARLITOL 25C, maltose, gluconic acid
Salt, malic acid, citric acid, phenylacetic acid, L-arabinose cannot utilize capric acid, adipic acid, D-MANNOSE, N- acetyl-glucose
Amine, beta galactosidase.
API ZYM are the results show that bacterial strain DYS3-4 has alkaline phosphatase, esterase, leucine amino peptidase, valine ammonia peptide
Enzyme, cystine aminopeptidase, trypsase, acid phosphatase, α-Portugal (grape) glycosidase do not have, β-Portugal (grape) glycosidase, α-half
Lactoside enzyme, beta galactosidase, alpha -chymotrypsin, β-Portugal (grape) glycuronides enzyme, N- acetyl-glucosaminidase, α-are sweet
Reveal the enzymatic activitys such as glycosidase, Alpha-Fucosidase.
Molecular Identification：It compares and analyzes through BLAST, the 16S rRNA genes of arthrobacterium (Arthrobacter sp.) DYS3-4
With Arthrobacter nicotianae DSM 20123 (T) X80739 similitude highests, respectively 99.14%, be secondly
A.arilaitensis Re117 (T) FQ311875 and A.soli SYB2 (T) EF660748, respectively 98.93% He
98.21%.
Morphological feature, physio-biochemical characteristics and the 16S rRNA gene sequencings of comprehensive bacterial strain are as a result, the present invention
Arthrobacterium (Arthrobacter sp.) DYS3-4 belongs to Arthrobacter, specially arthrobacterium (Arthrobacter sp.)
DYS3-4.The 16S rRNA gene orders of arthrobacterium (Arthrobacter sp.) DYS3-4 are as shown in SEQ ID NO.1.
Denitrification effects of 2 arthrobacterium of embodiment (Arthrobacter sp.) DYS3-4 to NO3-N and NO2-N
Fresh thalline is scraped from MA tablets, is resuspended with sterile saline, and strain concentration is adjusted to 109Cfu/mL is pressed
It is inoculated into the NO containing 100mg/L according to 2% inoculum concentration3 -- N or NO2 -In the nitrogenous simulated wastewater of-N, at 30 DEG C, 160r/
It is cultivated in min shaking tables, every the concentration of 4h sample detection waste water nitrites and nitrate, cell concentration OD600, measure bacterium
The denitrification effect of strain.Simulated wastewater ingredient is as follows：Sodium succinate 3.5g/L, sodium citrate 2.5g/L, sodium acetate 1g/L, glucose
5g/L, KH2PO42g/L, Na2HPO43g/L, MgSO4·7H2O 0.05g/L、FeSO4·7H2O 0.02g/L、CaCL20.02g/
L, distilled water 1000mL, 121 DEG C of sterilizing 20min.Nitrate nitrogen is measured to be measured using GB T12763 Zinc Cadmium Reductions；Nitrous acid
Salt nitrogen determination uses mono- hydrochloride naphthodiamide spectrophotometry of GB 7493-87.The result shows that bacterial strain DYS3-4 respectively in 12h and
The NO3-N and NO2-N in waste water can be removed completely when 16h.
3 arthrobacterium of embodiment (Arthrobacter sp.) DYS3-4 is at different temperatures to the waste water containing nitrite
Denitrification effect
Method is the same as embodiment 2.Arthrobacterium (Arthrobacter sp.) DYS3-4 is inoculated in 100mg/L NO2 -- N is
In the simulated wastewater of only nitrogen source, different temperatures (5 DEG C, 10 DEG C, 15 DEG C, 25 DEG C, 30 DEG C, 35 DEG C, 40 DEG C, 45 DEG C) is set,
Bacterium turbidity (the OD in waste water is measured by sampling every 12h in 160r/min shake cultures600) and nitrite concentration.The result is shown in Figure 1
~5, the results showed that at 15 DEG C, 25 DEG C, 28 DEG C and 35 DEG C, arthrobacterium (Arthrobacter sp.) DYS3-4 speeds of growth are fast,
With higher nitrite removal efficiency, difference can be in 72,48,48 and 60h by 100mg/L NO2 -- N is removed completely.
10 DEG C also can normal growth and denitrogenation, can be in 5d by 100mg/L NO2 -- N is removed completely.In addition, at 5 DEG C, it is right in 10 days
100mg/L NO2 -The removal rate of-N reaches 50%.
4 arthrobacterium of embodiment (Arthrobacter sp.) DYS3-4 is to higher concentration NO2 -- N tolerances and denitrification effect
Cultural method with embodiment 2, by arthrobacterium (Arthrobacter sp.) DYS3-4 be inoculated in 500mg/L,
1000mg/L with NO2 -- N is to be grown to arthrobacterium (Arthrobacter sp.) DYS3-4 in the simulated wastewater of only nitrogen source
Situation and nitrogen removal performance are measured.The result shows that arthrobacterium (Arthrobacter sp.) DYS3-4 at 25 DEG C, distinguishes energy
500mg/L, 1000mg/L nitrite nitrogen are removed completely in 84h and 120h.
Claims (4)
1. arthrobacterium (Arthrobacter sp.) DYS3-4 has been preserved in Chinese Typical Representative culture guarantor on 04 29th, 2015
Tibetan center, deposit number are CCTCC NO:M 2015264；Arthrobacterium (Arthrobacter sp.) DYS3-4 is nitrogenous
It is applied in wastewater biological denitrificaion；The nitrogenous effluent includes nitrate, nitrite, ammonia nitrogen waste water.
2. arthrobacterium (Arthrobacter sp.) DYS3-4 as described in claim 1 is answered in the removal of sea-farming nitrite
With.
3. arthrobacterium (Arthrobacter sp.) DYS3-4 as described in claim 1 is answered in the removal of freshwater aquiculture nitrite
With.
4. arthrobacterium (Arthrobacter sp.) DYS3-4 as described in claim 1 is in preparing low-temperature denitrification microecological microbial agent
Using.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510315944.2A CN104862260B (en) | 2015-06-10 | 2015-06-10 | One plant of arthrobacterium and its application with aerobic denitrification ability |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510315944.2A CN104862260B (en) | 2015-06-10 | 2015-06-10 | One plant of arthrobacterium and its application with aerobic denitrification ability |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104862260A CN104862260A (en) | 2015-08-26 |
| CN104862260B true CN104862260B (en) | 2018-09-25 |
Family
ID=53908441
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510315944.2A Active CN104862260B (en) | 2015-06-10 | 2015-06-10 | One plant of arthrobacterium and its application with aerobic denitrification ability |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104862260B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106148223B (en) * | 2016-05-06 | 2020-04-21 | 国家海洋局第一海洋研究所 | Arctic domestic sewage low-temperature efficient degrading bacterium and screening method and application thereof |
| CN106010981B (en) * | 2016-06-13 | 2019-05-07 | 淮阴师范学院 | One plant of nitrite degradation bacterium |
| CN106591181B (en) * | 2016-12-08 | 2019-02-26 | 河北大学 | A kind of Mysore arthrobacterium and its application in purifying sea water cultivation nitrogenous effluent |
| CN107245463B (en) * | 2017-06-22 | 2020-09-04 | 温州大学 | Heterotrophic nitrification-aerobic denitrification arthrobacter and application thereof |
| CN110184223B (en) * | 2019-06-17 | 2020-11-24 | 华中农业大学 | Arthrobacter calsium capable of removing ammonia nitrogen in culture sewage and application thereof |
| CN112029688B (en) * | 2020-09-24 | 2022-04-26 | 新疆水处理工程技术研究中心有限公司 | Nitrifying bacteria agent for industrial wastewater and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102465105A (en) * | 2010-11-04 | 2012-05-23 | 中国石油化工股份有限公司 | Nitrous acid-type denitrification bacteria strain and application thereof |
| CN102465101A (en) * | 2010-11-04 | 2012-05-23 | 中国石油化工股份有限公司 | Denitrification bacterium preparation capable of utilizing nitrite to realize denitrification and use thereof |
| CN104164390A (en) * | 2014-07-09 | 2014-11-26 | 青岛蔚蓝天成生物科技有限公司 | Arthrobacter nicotianae and application thereof in aquaculture |
-
2015
- 2015-06-10 CN CN201510315944.2A patent/CN104862260B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102465105A (en) * | 2010-11-04 | 2012-05-23 | 中国石油化工股份有限公司 | Nitrous acid-type denitrification bacteria strain and application thereof |
| CN102465101A (en) * | 2010-11-04 | 2012-05-23 | 中国石油化工股份有限公司 | Denitrification bacterium preparation capable of utilizing nitrite to realize denitrification and use thereof |
| CN104164390A (en) * | 2014-07-09 | 2014-11-26 | 青岛蔚蓝天成生物科技有限公司 | Arthrobacter nicotianae and application thereof in aquaculture |
Non-Patent Citations (3)
| Title |
|---|
| 1 株Arthrobacter arilaitensis 菌的耐冷异养硝化和好氧反硝化作用;何腾霞等;《环境科学》;20160226;第37卷(第3期);1082-1088 * |
| 低温好氧反硝化菌的分离鉴定及脱氮特性研究;魏巍等;《供水技术》;20121231;第6卷(第6期);12-16 * |
| 好氧反硝化微生物多样性及其反硝化功能初步研究;李小义等;《氨基酸和生物资源》;20160616;第38卷(第2期);37-45 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104862260A (en) | 2015-08-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104911130B (en) | One plant of Halomonas with denitrification ability and its application | |
| CN104862260B (en) | One plant of arthrobacterium and its application with aerobic denitrification ability | |
| CN104845920B (en) | One plant of ocean Zhuo Beier Salmonella and its application | |
| CN107201325A (en) | Pseudomonad strain and its cultural method and application | |
| CN105420165B (en) | One plant of aerobic denitrifying bacteria and its application | |
| CN102747014B (en) | High-saline biological denitrification salinivibrio strain and application thereof in wastewater treatment | |
| CN112625942B (en) | Aerobic denitrifying bacterium and application thereof | |
| CN103540544B (en) | Bacillus radicicola capable of degrading pyridine as well as breeding method and application thereof | |
| CN104152377A (en) | Heavy-metal-tolerant aerobic denitrification strain and application thereof | |
| CN101386823B (en) | Special effect anaerobic denitrifying bacterium and waste water processing method using thereof | |
| CN109337832A (en) | A kind of anthropi of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification and its application | |
| CN108865940A (en) | One plant of heterotrophic nitrification-aerobic denitrification bacillus and its composite bacteria preparation | |
| CN101811779B (en) | Preparation method of halophilic decontamination bacterial agent and bacterial agent prepared by same | |
| CN101538544B (en) | Composite microbial inoculum, preparation and application thereof | |
| CN112266885B (en) | Heterotrophic nitrification aerobic denitrifying bacteria Y16 and application thereof | |
| CN111996138B (en) | Reduced strain Ylb10 and application thereof in reduction of Cr (VI) | |
| CN104263686B (en) | One plant of low-temperature denitrification acinetobacter calcoaceticus and its application | |
| CN107794235B (en) | A kind of aerobic denitrifying bacteria and its application | |
| CN101811780B (en) | Preparation method and application of halophilic decontamination bacterial agent | |
| CN106591181A (en) | Arthrobactermysorens and application thereof to purification of marine-culture nitrogen-containing wastewater | |
| CN110628670B (en) | Heterotrophic nitrifying bacteria and application thereof | |
| CN103013867A (en) | Acid-producing klebsiella pneumoniae DF-1 and application thereof in removing nitrous nitrogen in water body | |
| CN101054241A (en) | Application of flavobacterium omnivorum in treating sewage at low temperature | |
| CN113005063B (en) | Pseudomonas putida GY13 and application thereof in sewage treatment | |
| CN116396912B (en) | Pyrethroid pesticide intermediate degrading bacterium, microbial inoculum and wastewater treatment method and treatment device thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |