CN101367581A - Composite microorganism formulation for controlling excessive multiplication of blue-green algae and preparation method thereof - Google Patents
Composite microorganism formulation for controlling excessive multiplication of blue-green algae and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a compound microbiological preparation and a preparation method for controlling the excessive reproduction of blue-green algae. The preparation comprises the following compounds with a certain weight proportion of microbial agents, organic fermentation agents, brown sugar and micro elements. The preparation steps are as follows: A. the preparation of the microbial agent, B. the preparation of the organic fermentation agent, and then the microbial agent\ fermentation agent, brown sugar and the micro elements are mixed based on a certain weight proportion, and finally, the compound microbiological preparation can be prepared. The preparation has reasonable formulation, is convenient to be used, has no pollution to the water, has high control rate and low cost, and is easy and convenient to be operated.
Description
Technical field
The invention belongs to microbial technology field, more specifically relate to a kind of complex microorganism preparations that is used to control water body blue-green algae excessive multiplication, the preparation method who also relates to this biotechnological formulation simultaneously, said preparation is applicable to aquaculture water blue-green algae excessive multiplications such as control lake, reservoir, pond, improves water quality.
Background technology
In recent years, along with ground water pollution and body eutrophication aggravation, in many lakes of China, reservoir, the highdensity aquaculture pond water body, the blue-green algae excessive multiplication also is a ubiquitous environmental problem.Water body blue-green algae excessive multiplication not only influences view, also has a strong impact on the water body functions of use, directly endangers breeding production simultaneously.Blue-green algae excessive multiplication phenomenon mainly is because nutrient concentration is excessive in the water body, ratio is improper, brings out at hot weather to cause.Control blue-green algae excessive multiplication phenomenon is an important topic of water environment protection.The technology that is used to eliminate water body blue-green algae excessive multiplication phenomenon at present comprises chemical algae removing method, physical method, ecological treatment method and microbial process etc.The chemical algae removing method adds algicide exactly in water body, this method is got instant result, but is difficult to effect a permanent cure, and secondary pollution is serious; In the chemical algae removing method, CN1214204 discloses a kind of " the powder algicide that rivers, lake, pond are used ", be used to eliminate the algae such as blue-green algae of rivers, lake, pond kind, this algicide adopts the flocculation of polymerize aluminum chloride, zeolite powder, adsorption to remove algae, the medicament usage quantity is big, and used polymerize aluminum chloride itself has certain harm to aquatic animal, and just simple except that algae, blue-green algae and other algae are together removed, unfavorable to the algae ecologic structure of water body; Physical method is installed means increase water bodys such as water pump, oxygenating machine exactly and is flowed, and reduces algae reproduction, and this method is a kind of assist measure usually, and energy consumption is bigger; The major technique means of ecological treatment method are the member waterplant, hydrocoles, and water body microecosystem, form hydrobiont chain preferably, make nutritive salt obtain benign cycle, keep the low nutrient concentration that reaches in the water body, finally reach the purpose of avoiding the blue-green algae excessive multiplication, ecological treatment method is a kind of ideal blue-green algae resolution to great water body, but to being subjected to the place, the small-sized polluted-water of fund limitation, engineering construction and management difficulty are bigger, microbial process is exactly by adding effective microorganism preparation, with blue-green algae contention nutrition, reduce blue-green algae density, this method is a kind of feature of environmental protection improvement method, non-secondary pollution, and input cost is little, and is effective.
Summary of the invention
The present invention uses chemical fertilizer, fertilizer in a large number at present raiser, cause water quality overfertilization, blue-green algae too much to bring out problems such as floating head and multiple disease, the objective of the invention is to be to provide a kind of complex microorganism preparations that is used to control excessive multiplication of blue-green algae, prescription rationally, easy to use, pollution-free to water body, the inverse amplification factor height, cost is low.
Another object of the present invention is to be to provide a kind of preparation method who is used to control the complex microorganism preparations of excessive multiplication of blue-green algae, and easy to implement the method, easy and simple to handle, production cost is low.
Technology of the present invention is achieved in that a kind of complex microorganism preparations, it is characterized in that it is formulated by following weight percentages:
Microbiobacterial agent 0.2%-35%, organic fermentation material 5%-90%, brown sugar 1%-50%, micro-0.1%-20%;
Described microbiobacterial agent is photosynthetic bacterium, milk-acid bacteria, yeast, nitrobacteria, actinomycetes, a Sheng nation water treatment element and water purification treasured at least a or arbitrary combination wherein;
Water treatment element production firm of described Sheng nation is Beijing associating Sheng Bang Bioisystech Co., Ltd, and containing nation's water treatment element is genus bacillus, photosynthetic bacterium, nitrifier, vinelandii, denitrifying bacteria at least a or arbitrary combination wherein;
The precious production firm of described water purification is the strong animal pharmaceutcal corporation, Ltd in sky, Yangzhou, and the water purification treasured is genus bacillus, photosynthetic bacterium, nitrococcus, actinomycetes, a denitrifying bacteria at least a or arbitrary combination wherein;
Described organic fermentation material is a wherein at least a or arbitrary combination of the dish dregs of rice, dregs of beans, oily chaff, wheat bran, soyflour, bone meal, fish meal, lime powder and terra alba;
Described trace element is copper, iron, manganese, zinc, silicon, iodine, a cobalt and boron at least a or arbitrary combination wherein;
A kind of proportioning of complex microorganism preparations, preferable range is:
Microbiobacterial agent 0.5%-30%, organic fermentation material 10%-85%, brown sugar 3%-40%, micro-0.5%-18%;
A kind of proportioning of complex microorganism preparations, optimum range is:
Microbiobacterial agent 1%-20%, organic fermentation material 20%-80%, brown sugar 4%-30%, micro-1%-15%;
A kind of complex microorganism preparations, its best proportioning is:
Microbiobacterial agent 10.5%, organic fermentation material 80%, brown sugar 6.5%, trace element 3%;
Microbiobacterial agent 10%, organic fermentation material 80%, brown sugar 5%, trace element 5%;
Microbiobacterial agent 10%, organic fermentation material 75%, brown sugar 8%, trace element 7%;
Microbiobacterial agent 5%, organic fermentation material 80%, brown sugar 10%, trace element 5%;
Microbiobacterial agent 15%, organic fermentation material 75%, brown sugar 3%, trace element 7%;
Microbiobacterial agent 8%, organic fermentation material 70%, brown sugar 15%, trace element 7%;
Wherein the preparation method of microbiobacterial agent the steps include:
The cultivation of A, photosynthetic bacterium: photosynthetic bacterium stoste is inoculated in the liquid test tube with cover, 28-30 ℃, illumination cultivation 3-5 days, gets the photosynthetic bacterium bacteria suspension; Get the bacteria suspension of 0.1-1% and put into the substratum of 500ml triangular flask deactivation, with improvement Fan Shi substratum (seeing microbiology experiment textbook) multiplication culture, 28-30 ℃ of constant temperature vibration shaking table, cultivated 36-72 hour, get the secondary pure growth of photosynthetic bacterium, the photosynthetic bacterium secondary seed of getting 0.5-1% is inoculated in the sodium acetate by 0.3-5%, 0.12-2% ammonium chloride, the 0.06-0.1% potassium primary phosphate, 0.01-0.5% sal epsom, 0.01-0.2 sodium-chlor, the 0.05-4% amine molybdate, the 0.01-0.05% yeast extract, 0.01-0.05 calcium chloride, fermentation propagation in the substratum that 0.01-0.05% iron(ic) chloride is formed, 28-30 ℃ of static cultivation of illumination or not illumination shaking culture, get liquid photosynthetic bacterium fermenting agent, again liquid photosynthetic bacterium fermenting agent is inoculated on the solid medium, gets the bacterium number and reach 1.0-2.0 * 10
8The solid photosynthetic bacteria fermenting agent of individual/ml;
The cultivation of B, milk-acid bacteria: milk-acid bacteria is on malt extract medium (seeing microbiology experiment textbook), 28-30 ℃, make primary inclined plane and cultivate, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-5.0 * 10
8Individual/the gram solid medium;
C, saccharomycetic cultivation: milk-acid bacteria is on lactic acid-potato-dextrose culture-medium (seeing microbiology experiment textbook), 28-30 ℃, making primary inclined plane cultivates, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
The cultivation of D, nitrobacteria: nitrobacteria comprises nitrococcus and nitrifier, respectively the ammonia-state nitrogen in the water is converted into nitrite and nitrate, 24 ℃, making primary inclined plane cultivates, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
Described nitrococcus substratum: ammonium sulfate (NH4)
2SO
40.5g, sodium chloride nacl 0.3g, ferrous sulfate FeSO
47H
2O 0.03g, dipotassium hydrogen phosphate K
2HPO
41g, sal epsom MgSO
47H
2O 0.03g, calcium chloride CaCl
27.5g, distilled water 1000ml, PH nature, solid medium adds 5% agar;
Described nitrifier substratum: Sodium Nitrite NaNO
21g, sal epsom MgSO
47H
2O 0.03g, manganous sulfate MnSO
44H
2O 0.01g, dipotassium hydrogen phosphate K
2HPO
40.75g, yellow soda ash Na
2CO
31g, SODIUM PHOSPHATE, MONOBASIC NaH
2PO
40.25g, distilled water 1000ml, PH nature, solid medium adds 5% agar;
E, actinomycetic cultivation: actinomycetes are at first on Gause I substratum (seeing microbiology experiment textbook), 28-30 ℃, making primary inclined plane cultivates, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium.
The preparation method of wherein organic fermentation material the steps include:
A, match and comprise wherein at least a or arbitrary combination raw material of the dish dregs of rice, dregs of beans, oily chaff, wheat bran, soyflour, bone meal, fish meal, lime powder and terra alba;
B, pulverizing: various raw materials are crushed to the 45-50 order with pulverizer;
C, combined inoculation; The raw material that crushes is mixed, (described enzymatic microorganism is that (production firm is the Japanese JATANSHIMAMOTODISEIBUTUKOUTYOCO of manufacturer to company's introduction enzymatic microorganism original seed to inoculation enzymatic microorganism microbial inoculum, .LTD) after, through enlarged culturing form " close edge: the board enzymatic microorganism enlarges bacterium; it is bacterium (Becteria.B) that enzymatic microorganism enlarges bacterium, yeast (Yest, Y), thread fungus (Mold; M) and actinomycetes at least a or arbitrary combination wherein), stir;
D, fermentation: the material that mixes is fermented, and described fermentation is an aerobic fermentation, and moisture content accounts for the 15%-30% of material gross weight, and fermentation time is 48-96 hour, and material produces till the wine flavour;
E, drying, packing: will ferment material carry out drying, drying temperature is 20-58 ℃, dry back water content is 10%-15%;
To at first mix by above cultured microbiobacterial agent, and then mix in proportion at normal temperatures, just obtain complex microorganism preparations with the organic fermentation material, brown sugar, the trace element that ferment.
Above-mentioned microbiobacterial agent is mainly used in and improves water quality, control water body blue-green algae excessive multiplication.
Using method: select the fine morning, mu uses 5-8KG, and with 100 times of clear water dilutions, evenly splashing gets final product in the pond, generally weekly.
Advantage of the present invention is: be referred from blue-green algae biology newest research results, profitable strains such as photosynthetic bacterium, milk-acid bacteria, yeast, nitrobacteria, actinomycetes are carried out scientific and reasonable integration, a kind of complex micro organism fungicide that the uses advanced production technique produces.These cultivate the complex micro organism fungicide form through special methods, have share weal or woe, have complementary functions, the characteristics of cooperation.After applying water body, utilize the microorganism metabolic activity, to organic pollutant in the water shift, conversion and Degradation, reduce water pollutant concentration, cut off the nutrition supply of harmful algae.And objectionable impuritiess such as ammonia nitrogen, nitrous acid, hydrogen sulfide in the collaborative water of decomposition environment, improve the anaerobic environment at the bottom of the pond; Improve the absorb efficient of unicellular algae to nutritive element, promote to swim but the growth of born of the same parents algae, balance algae phase increases the algae diversity, suppresses the particularly excessive breeding of blue-green algae of algae in the aquaculture water, suppresses the growth of harmful bacterium.Photosynthetic bacteria utilizes in luminous energy and the water simple inorganics to carry out photosynthesis, fixedly in the water element such as nitrogen and phosphorus the time, produces oxygen, and increases oxygen level in the water body, the raising water transparency; Stablize water pH value.Water body biology especially nanoplankton diversity index improves constantly, and for fish provide good natural bait, is absorbed in a large number by fish, accelerates the matter and energy circulation of water body.Remove water surface sweet wormwood, vacuolar membrane and cake mass, prevent that water quality from thickening, blackening, redden, feculence, keep culturing the balance of little ecology, make aquaculture water present " fertilizer ", " work ", " tender ", " feeling well "; Using method is simple simultaneously, and is convenient, reduces labour cost.
Embodiment
Embodiment 1:
A kind of complex microorganism preparations that is used to control excessive multiplication of blue-green algae, its proportioning is as follows:
Microbiobacterial agent 12%, organic fermentation material 77%, brown sugar 6%, trace element 5%;
According to described various raw-material mass percents, following concrete technical scheme is arranged:
A kind of preparation method who is used to control the complex microorganism preparations of excessive multiplication of blue-green algae the steps include:
The cultivation of A, photosynthetic bacterium: photosynthetic bacterium stoste is inoculated in the liquid test tube with cover, 28-30 ℃, illumination cultivation 3-5 days, gets the photosynthetic bacterium bacteria suspension; Get the bacteria suspension of 0.1-1% and put into the substratum of 500ml triangular flask deactivation, with improvement Fan Shi substratum (seeing microbiology experiment textbook) multiplication culture, 28-30 ℃ of constant temperature vibration shaking table, cultivated 36-72 hour, get the secondary pure growth of photosynthetic bacterium, the photosynthetic bacterium secondary seed of getting 0.5-1% is inoculated in the sodium acetate by 0.3-5%, 0.12-2% ammonium chloride, the 0.06-0.1% potassium primary phosphate, 0.01-0.5% sal epsom, 0.01-0.2 sodium-chlor, the 0.05-4% amine molybdate, the 0.01-0.05% yeast extract, 0.01-0.05 calcium chloride, fermentation propagation in the substratum that 0.01-0.05% iron(ic) chloride is formed, 28-30 ℃ of static cultivation of illumination or not illumination shaking culture, get liquid photosynthetic bacterium fermenting agent, again liquid photosynthetic bacterium fermenting agent is inoculated on the solid medium, gets the bacterium number and reach 1.0-2.0 * 10
8The solid photosynthetic bacteria fermenting agent of individual/ml;
The cultivation of B, milk-acid bacteria: milk-acid bacteria is on malt extract medium (seeing microbiology experiment textbook), 28-30 ℃, make primary inclined plane and cultivate, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-5.0 * 10
8Individual/the gram solid medium;
C, saccharomycetic cultivation: milk-acid bacteria is on lactic acid-potato-dextrose culture-medium (seeing microbiology experiment textbook), 28-30 ℃, making primary inclined plane cultivates, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
The cultivation of D, nitrobacteria: nitrobacteria comprises nitrococcus and nitrifier, respectively the ammonia-state nitrogen in the water is converted into nitrite and nitrate, 24 ℃, making primary inclined plane cultivates, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
Described nitrococcus substratum: ammonium sulfate (NH4)
2SO
40.5g, sodium chloride nacl 0.3g, ferrous sulfate FeSO
47H
2O 0.03g, dipotassium hydrogen phosphate K
2HPO
41g, sal epsom MgSO
47H
2O 0.03g, calcium chloride CaCl
27.5g, distilled water 1000ml, PH nature, solid medium adds 5% agar;
Described nitrifier substratum: Sodium Nitrite NaNO
21g, sal epsom MgSO
47H
2O 0.03g, manganous sulfate MnSO
44H
2O 0.01g, dipotassium hydrogen phosphate K
2HPO
40.75g, yellow soda ash Na
2CO
31g, SODIUM PHOSPHATE, MONOBASIC NaH
2PO
40.25g, distilled water 1000ml, PH nature, solid medium adds 5% agar;
E, actinomycetic cultivation: actinomycetes are at first on Gause I substratum (seeing microbiology experiment textbook), 28-30 ℃, making primary inclined plane cultivates, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium.
The preparation method of wherein organic fermentation material the steps include:
A, match and comprise wherein at least a or arbitrary combination raw material of the dish dregs of rice, dregs of beans, oily chaff, wheat bran, soyflour, bone meal, fish meal, lime powder and terra alba;
B, pulverizing: various raw materials are crushed to the 45-50 order with pulverizer;
C, combined inoculation; The raw material that crushes is mixed, and inoculation enzymatic microorganism microbial inoculum (described enzymatic microorganism is bacterium, yeast, a thread fungus and actinomycetes at least a or arbitrary combination wherein) stirs;
D, fermentation: the material that mixes is fermented, and described fermentation is an aerobic fermentation, and moisture content accounts for the 15%-30% of material gross weight, and fermentation time is 48-96 hour, and material produces till the wine flavour;
E, drying, packing: will ferment material carry out drying, drying temperature is 20-58 ℃, dry back water content is 10%-15%;
To at first mix by above cultured microbiobacterial agent, and then mix in proportion at normal temperatures, just obtain complex microorganism preparations with the organic fermentation material, brown sugar, the trace element that ferment.
Test case 1:
Jiangxia, test site Wuhan, 2 mu of water surfaces for aquaculture, 1.5 meters of the depth of waters, before using, the water body algae is mutually mainly based on blue-green algae, and water body is aging, and transparency has only 15cm.Used microbiobacterial agent on June 18th, 2008, mu uses 8KG, with 100 times of clear water dilutions, evenly splashes in the pond, and after 7 days, transparency is brought up to 35cm; Dissolved oxygen rises to 1299mg/L from 772mg/L; Total N, total P content and permanganate have descended 30.2%, 28.1% and 17.2% respectively, and blue-green algae quantity is from 53000 * 10
3Individual/L reduces to 9900 * 10
3Individual/L, water body be improved significantly.
Test case 2:
Test site Hunan Ma Kou, 80 mu of water surfaces for aquaculture, depth of water 1.5-2 rice, before using, the water body algae is mutually mainly based on blue-green algae, blue-green algae quantity 27700 * 10
3Individual/L, water body is aging, and transparency is 25cm; Used microbiobacterial agent on July 4th, 2008, mu uses 5KG, with 100 times of clear water dilutions, evenly splashes in the pond, and after 5 days, transparency is brought up to 40cm; Dissolved oxygen rises to 1896mg/L from 927mg/L; Blue-green algae quantity reduces to 5200 * 10
3Individual/L, water body be improved significantly.
Test case 3:
Shayang County, test site Jingmen, 300 mu of water surfaces for aquaculture, depth of water 1.5-2 rice, before using, the water body algae is mutually mainly based on blue-green algae, blue-green algae quantity 32100 * 10
3Individual/L, water body is aging, and transparency is 20cm, and fish raises the nose above water to breathe in a large number; Used microbiobacterial agent on July 12nd, 2008, mu uses 6KG, with 100 times of clear water dilutions, evenly splashes in the pond, and after 7 days, transparency is brought up to 40cm; Blue-green algae quantity reduces to 8600 * 10
3Individual/L, other zooplanktons such as wheel animalcule, cladocera animal are increased, and the water body biological cycle is accelerated, and fish raises the nose above water to breathe phenomenon and disappears substantially, water body be improved significantly.
Test case 4:
Test site Wuhan Hong Shan, 220 mu of water surfaces for aquaculture, depth of water 2-3 rice, before using, the water body algae is mutually mainly based on blue-green algae, blue-green algae quantity 41200 * 10
3Individual/L, water body is aging, and transparency is 15cm, and fish raises the nose above water to breathe in a large number; Used microbiobacterial agent on July 18th, 2008, mu uses 8KG, with 100 times of clear water dilutions, evenly splashes in the pond, and after 7 days, transparency is brought up to 30cm; Blue-green algae quantity reduces to 12800 * 10
3Individual/L, other zooplanktons such as wheel animalcule, cladocera animal are increased, and the water body biological cycle is accelerated, and fish raises the nose above water to breathe phenomenon and disappears substantially, water body be improved significantly.
Claims (10)
1. complex microorganism preparations that is used to control excessive multiplication of blue-green algae, it is formulated by following weight percentages:
Microbiobacterial agent 0.2%-35%, organic fermentation material 5%-90%, brown sugar 1%-50%, micro-0.1%-20%;
Described microbiobacterial agent is photosynthetic bacterium, milk-acid bacteria, yeast, nitrobacteria, actinomycetes, a Sheng nation water treatment element and water purification treasured at least a or arbitrary combination wherein;
Described Sheng nation water treatment element is genus bacillus, photosynthetic bacterium, nitrifier, vinelandii, a denitrifying bacteria at least a or arbitrary combination wherein;
Described water purification treasured is genus bacillus, photosynthetic bacterium, nitrococcus, actinomycetes, a denitrifying bacteria at least a or arbitrary combination wherein;
Described organic fermentation material is a wherein at least a or arbitrary combination of the dish dregs of rice, dregs of beans, oily chaff, wheat bran, soyflour, bone meal, fish meal, lime powder and terra alba;
Described trace element is copper, iron, manganese, zinc, silicon, iodine, a cobalt and boron at least a or arbitrary combination wherein.
2. a kind of complex microorganism preparations that is used to control excessive multiplication of blue-green algae according to claim 1 is characterized in that it is formulated by following materials by weight percentage:
Microbiobacterial agent 0.2%-35%, organic fermentation material 10%-90%, brown sugar 1%-50%, micro-0.1%-20%.
3. a kind of complex microorganism preparations that is used to control excessive multiplication of blue-green algae according to claim 1 is characterized in that it is formulated by following materials by weight percentage:
Microbiobacterial agent 0.2%-30%, organic fermentation material 15%-80%, brown sugar 2%-45%, micro-0.1%-20%.
4. a kind of complex microorganism preparations that suppresses excessive multiplication of blue-green algae according to claim 1 is characterized in that it is formulated by following materials by weight percentage:
Microbiobacterial agent 0.5%-28%, organic fermentation material 20%-80%, brown sugar 3%-40%, micro-0.1%-18%.
5. a kind of complex microorganism preparations that is used to control excessive multiplication of blue-green algae according to claim 1 is characterized in that it is formulated by following materials by weight percentage:
Microbiobacterial agent 1%-28%, organic fermentation material 25%-75%, brown sugar 3%-35%, micro-0.5%-18%.
6. a kind of complex microorganism preparations that is used to control excessive multiplication of blue-green algae according to claim 1 is characterized in that it is formulated by following materials by weight percentage:
Microbiobacterial agent 1%-25%, organic fermentation material 30%-75%, brown sugar 3%-30%, micro-0.5%-15%.
7. a kind of complex microorganism preparations that is used to control excessive multiplication of blue-green algae according to claim 1 is characterized in that it is formulated by following materials by weight percentage:
Microbiobacterial agent 5%, organic fermentation material 70%, brown sugar 15%, trace element 10%.
8. a kind of complex microorganism preparations that is used to control excessive multiplication of blue-green algae according to claim 1 is characterized in that it is formulated by following materials by weight percentage:
Microbiobacterial agent 10%, organic fermentation material 65%, brown sugar 13%, trace element 12%.
9. one kind prepares the described a kind of method that is used to control the complex microorganism preparations of excessive multiplication of blue-green algae of claim 1, the steps include:
The cultivation of A, photosynthetic bacterium: photosynthetic bacterium stoste is inoculated in the liquid test tube with cover, 28-30 ℃, illumination cultivation 3-5 days, gets the photosynthetic bacterium bacteria suspension; Get the bacteria suspension of 0.1-1% and put into the substratum of 500ml triangular flask deactivation, with improvement Fan Shi substratum multiplication culture, 28-30 ℃ of constant temperature vibration shaking table, cultivated 36-72 hour, get the secondary pure growth of photosynthetic bacterium, the photosynthetic bacterium secondary seed of getting 0.5-1% is inoculated in the sodium acetate by 0.3-5%, 0.12-2% ammonium chloride, the 0.06-0.1% potassium primary phosphate, 0.01-0.5% sal epsom, 0.01-0.2 sodium-chlor, the 0.05-4% amine molybdate, the 0.01-0.05% yeast extract, 0.01-0.05 calcium chloride, fermentation propagation in the substratum that 0.01-0.05% iron(ic) chloride is formed, 28-30 ℃ of static cultivation of illumination or not illumination shaking culture, get liquid photosynthetic bacterium fermenting agent, again liquid photosynthetic bacterium fermenting agent is inoculated on the solid medium, gets the bacterium number and reach 1.0-2.0 * 10
8The solid photosynthetic bacteria fermenting agent of individual/ml;
The cultivation of B, milk-acid bacteria: milk-acid bacteria on malt extract medium, 28-30 ℃, make primary inclined plane and cultivate, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-5.0 * 10
8Individual/the gram solid medium;
C, saccharomycetic cultivation: milk-acid bacteria is on lactic acid-potato-dextrose culture-medium, 28-30 ℃, make primary inclined plane and cultivate, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
The cultivation of D, nitrobacteria: nitrobacteria comprises nitrococcus and nitrifier, respectively the ammonia-state nitrogen in the water is converted into nitrite and nitrate, 24 ℃, making primary inclined plane cultivates, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
Described nitrococcus substratum: ammonium sulfate
2SO
40.5g, sodium-chlor 0.3g, ferrous sulfate 0.03g, dipotassium hydrogen phosphate 1g, sal epsom 0.03g, calcium chloride 7.5g, distilled water 1000ml, PH nature, solid medium add 5% agar;
Described nitrifier substratum: Sodium Nitrite 1g, sal epsom 0.03g, manganous sulfate 0.01g, dipotassium hydrogen phosphate 0.75g, yellow soda ash 1g, SODIUM PHOSPHATE, MONOBASIC N0.25g, distilled water 1000ml, PH nature, solid medium add 5% agar;
E, actinomycetic cultivation: actinomycetes at first on the Gause I substratum, 28-30 ℃, make primary inclined plane and cultivate, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium.
10. one kind prepares the described a kind of method that is used to control the complex microorganism preparations of excessive multiplication of blue-green algae of claim 1, the steps include:
A, match and comprise wherein at least a or arbitrary combination raw material of the dish dregs of rice, dregs of beans, oily chaff, wheat bran, soyflour, bone meal, fish meal, lime powder and terra alba;
B, pulverizing: various raw materials are crushed to the 45-50 order with pulverizer;
C, combined inoculation; The raw material that crushes is mixed, and inoculation enzymatic microorganism microbial inoculum stirs;
Described enzymatic microorganism is bacterium, yeast, a thread fungus and actinomycetes at least a or arbitrary combination wherein;
D, fermentation: the material that mixes is fermented, and described fermentation is an aerobic fermentation, and moisture content accounts for the 15%-30% of material gross weight, and fermentation time is 48-96 hour, and material produces till the wine flavour;
E, drying, packing: will ferment material carry out drying, drying temperature is 20-58 ℃, dry back water content is 10%-15%;
At first mix by cultured microbiobacterial agent, and then mix in proportion at normal temperatures, just obtained complex microorganism preparations with the organic fermentation material, brown sugar, the trace element that ferment.
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Cited By (19)
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| CN101607761B (en) * | 2009-07-09 | 2011-06-01 | 东莞圣源环保科技有限公司 | Purification method of polluted lake water body |
| CN102381797A (en) * | 2011-10-14 | 2012-03-21 | 龙源海洋生物股份有限公司 | Method for treating waste liquid and waste gas produced by fish meal processing |
| CN102649603A (en) * | 2011-02-24 | 2012-08-29 | 上海三智生物科技有限公司 | High-activity and high-efficiency solid biological substrate-improving granule |
| CN102910743A (en) * | 2012-10-25 | 2013-02-06 | 中国水产科学研究院南海水产研究所 | Method for microcystis algae bloom treatment in earth pond cultivation process |
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| CN105217801A (en) * | 2015-09-11 | 2016-01-06 | 中国环境科学研究院 | The application of environmental protection ferment in control lake algal bloom harm |
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| CN112011486A (en) * | 2020-09-09 | 2020-12-01 | 天津市农业科学院 | Synchronous nitrification and denitrification bacillus subtilis K8 and application thereof |
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| CN101607761B (en) * | 2009-07-09 | 2011-06-01 | 东莞圣源环保科技有限公司 | Purification method of polluted lake water body |
| CN102010257B (en) * | 2010-06-09 | 2013-07-03 | 成都愷伟酵素矿物肥高新技术研究所 | Enzyme biological organic fertilizer applicable to rice field eels and loaches and preparation method thereof |
| CN102010257A (en) * | 2010-06-09 | 2011-04-13 | 徐维康 | Enzyme biological organic fertilizer applicable to rice field eels and loaches and preparation method thereof |
| CN102649603A (en) * | 2011-02-24 | 2012-08-29 | 上海三智生物科技有限公司 | High-activity and high-efficiency solid biological substrate-improving granule |
| CN102381797A (en) * | 2011-10-14 | 2012-03-21 | 龙源海洋生物股份有限公司 | Method for treating waste liquid and waste gas produced by fish meal processing |
| CN102910743A (en) * | 2012-10-25 | 2013-02-06 | 中国水产科学研究院南海水产研究所 | Method for microcystis algae bloom treatment in earth pond cultivation process |
| CN103224887B (en) * | 2013-05-07 | 2014-08-27 | 山东省环境保护科学研究设计院 | Preparation method of microbial nutrition agent for wastewater biochemical treatment |
| CN103224887A (en) * | 2013-05-07 | 2013-07-31 | 山东省环境保护科学研究设计院 | Preparation method of microbial nutrition agent for wastewater biochemical treatment |
| CN103641229A (en) * | 2013-11-14 | 2014-03-19 | 钟国防 | Method for regulating and controlling of water quality of Litopenaeus Vannamei culture pond |
| CN103641229B (en) * | 2013-11-14 | 2015-06-03 | 钟国防 | Method for regulating and controlling of water quality of Litopenaeus Vannamei culture pond |
| CN103588304A (en) * | 2013-11-27 | 2014-02-19 | 中国水产科学研究院珠江水产研究所 | Carbon source used for regulation and control of microbial community in culture pond water body and application |
| CN103588304B (en) * | 2013-11-27 | 2015-07-29 | 中国水产科学研究院珠江水产研究所 | A kind of cultivating pool Aquatic Microbiota group that is used for regulates and controls carbon source and application thereof |
| CN103723837A (en) * | 2013-12-04 | 2014-04-16 | 刘军亮 | Method for ecologically restoring polluted water by applying compound microorganism technology |
| CN103936122A (en) * | 2014-04-18 | 2014-07-23 | 芜湖凯奥尔环保科技有限公司 | Deodorizing powdery blue algae treating agent and production method thereof |
| CN103936122B (en) * | 2014-04-18 | 2015-12-02 | 芜湖凯奥尔环保科技有限公司 | A kind of deodorizing powdery blue algae treatment agent and preparation method thereof |
| CN104591482A (en) * | 2014-12-25 | 2015-05-06 | 苏州市阳澄湖现代农业发展有限公司 | Oxygenation treatment method for aquaculture water body |
| CN104609570A (en) * | 2015-01-07 | 2015-05-13 | 王迎春 | Method for treating algae-containing water bodies by utilizing mineral algae decomposition accelerator with compound microorganism in assistant manner |
| CN105154361A (en) * | 2015-08-31 | 2015-12-16 | 湖北中扬生态农业有限公司 | Micro-ecological preparation for medium-sized and small-sized reservoir ecological aquaculture feed plankton cultivation |
| CN105154361B (en) * | 2015-08-31 | 2018-09-18 | 湖北中扬生态农业有限公司 | The probiotics cultivated for medium and small reservoirs ecologic breeding bait planktonic organism |
| CN105217801A (en) * | 2015-09-11 | 2016-01-06 | 中国环境科学研究院 | The application of environmental protection ferment in control lake algal bloom harm |
| CN105217801B (en) * | 2015-09-11 | 2018-04-17 | 中国环境科学研究院 | Application of the environmental protection ferment in terms of lake algal bloom harm is prevented |
| CN105200019A (en) * | 2015-09-11 | 2015-12-30 | 中国环境科学研究院 | Preparation methods for garbage enzyme and algal bloom inhibitor |
| CN105200019B (en) * | 2015-09-11 | 2019-01-01 | 中国环境科学研究院 | The preparation method of environmental protection ferment and algal tufa inhibitor |
| CN105347511A (en) * | 2015-11-30 | 2016-02-24 | 威海地宝生物科技有限公司 | Compound microbial preparation for aquaculture and method |
| CN109437408A (en) * | 2018-10-19 | 2019-03-08 | 安徽瀚沣渔业科技发展有限公司 | A kind of beneficial cenobium except cyanobacteria |
| CN112011486A (en) * | 2020-09-09 | 2020-12-01 | 天津市农业科学院 | Synchronous nitrification and denitrification bacillus subtilis K8 and application thereof |
| CN112011486B (en) * | 2020-09-09 | 2022-03-01 | 天津市农业科学院 | Synchronous nitrification and denitrification bacillus subtilis K8 and application thereof |
| CN113151033A (en) * | 2020-12-17 | 2021-07-23 | 湖北绿天地生物科技有限公司 | Compound microbial preparation for inhibiting and killing blue algae and preparation method thereof |
| CN113151033B (en) * | 2020-12-17 | 2023-03-10 | 湖北绿天地生物科技有限公司 | Compound microbial preparation for inhibiting and killing blue algae and preparation method thereof |
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Address after: 430070 Hubei city of Wuhan province Luo Shi Road No. 517 building 503 room C Patentee after: WUHAN HEYUAN GREEN ORGANISM Co.,Ltd. Address before: 430070 Hubei city of Wuhan province Luo Shi Road No. 517 building 503 room C Patentee before: Wuhan Heyuan Green Bioengineering Co.,Ltd. |
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