CN101333502A - Process for preparing emergency strain and repairing aquaculture environment - Google Patents
Process for preparing emergency strain and repairing aquaculture environment Download PDFInfo
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- CN101333502A CN101333502A CNA2007100427793A CN200710042779A CN101333502A CN 101333502 A CN101333502 A CN 101333502A CN A2007100427793 A CNA2007100427793 A CN A2007100427793A CN 200710042779 A CN200710042779 A CN 200710042779A CN 101333502 A CN101333502 A CN 101333502A
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Abstract
Disclosed is an emergency bacterium which is an anti-stress product developed for solving the problem of stress response to aquaculture animals caused by the mutation of a plurality of environmental factors such as weather, water temperature, salinity, dissolved oxygen, algal facies and diseases by adopting techniques of microbial engineering, biophysical engineering, etc. Beneficial bacteria that are specially screened can rapidly decompose mass mucus produced on gills and skin surfaces of aquaculture animals due to stress response, and can rapidly absorb vitamin and polysaccharose substances under the action of electrolyte for filling the life energy, the beneficial bacteria and the polysaccharide can directly stimulate the aquaculture animals to produce interferon and increase the concentration of immunoglobulin and activity of macrophage, thereby enhancing the body immunity. The product also has high-efficient detoxification capacity, and can effectively reduce the toxicity of harmful substances of ammonia nitrogen, nitrite, sulfureted hydrogen, algal toxins, heavy metal ion, drug residue, etc. in water. The product has no toxic and side-effect to the environment, human, livestock, aquatic animals and plants, and is an essential article for recovering the aquiculture ecological environment and producing green aquatic products.
Description
Emergency strain be adopt technology such as microbial project and biophysics engineering solve because of weather, water temperature, salinity, dissolved oxygen, algae mutually, various environmental factors such as the disease a kind of anti-stress product that the stress reaction that causes to the aquatic products cultivated animals is developed that suddenlys change.Can decompose a large amount of mucus that aquatic animal produces at the gill and skin surface in response to swashing reaction fast through the probiotics of special screening, and under electrolytical effect, absorb rapidly VITAMIN and polysaccharose substance, replenish life energy, probiotics and polysaccharide can directly stimulate aquatic animal to produce Interferon, rabbit, improve the activity of immunoglobulin (Ig) concentration and scavenger cell, thus enhancing body immunizing power.This product also has the high-efficiency detoxication ability, can effectively reduce the toxicity of objectionable impuritiess such as ammonia nitrogen in the water body, nitrite, hydrogen sulfide, algae toxin, heavy metal ion, drug residue.This product is all without any side effects to environment, people, animal, aquatic animal and plant, is repairing aquaculture ecotope, the essential product of producing green aquatic product.
[effect]
1, alleviates the stress reaction of the aquatic animal that causes because of the sudden change of environmental factors such as weather, water temperature, salinity, dissolved oxygen, algae phase, disease fast;
2, promote aquatic animal to trace elements absorbed utilizations such as calcium, magnesium, phosphorus, promote growth;
3, decompose poisonous, objectionable impurities in the water body fast, improve water quality environment.
[physicochemical character] pale yellow powder has acescency.
[main component] milk-acid bacteria, yeast, actinomycetes, thread fungus, vitamins C, glucose, ammonia chloride, sodium bicarbonate, dipotassium hydrogen phosphate, sodium-acetate, magnesium chloride, sodium-chlor.
[active constituent content] every gram contains probiotic bacterium 2 * 10
8Individual.
[use range] is applicable to all seas, fresh water aquatic animal nursery pond and growing pond.
[usage] evenly splashes with full pond, 10-20 times of Chi Shui dilution back or mixed feed is thrown something and fed.
[consumption]
1, improve water quality: every mu of water surface (1 meter of the depth of water) restrains with this product 200-250, multiplicable splashing when serious.
2, mixed feed uses: every double centner feed uses the 250-500 gram, is mainly used in to strengthen the cultivated animals immunological competence.
[attention]
Forbid when 1, using " emergency strain " that the medicine that has germicidal action with microbiotic, sterilizing agent and all uses simultaneously;
2, use in " emergency strain " process, the dissolved oxygen in water amount should remain on more than 3 mg/litre;
3, use " emergency strain " not to be subjected to the influence of weather, water temperature, often use can guarantee that water quality is good;
4, " emergency strain " is safe in utilization, multiplicable use during severe contamination that cultivation water too much causes because of Changes in weather, bait feeding.
5, " emergency strain " should seal preservation, avoids illumination;
[summary of the invention]
The preparation method of aquatic products probiotics emergency strain of the present invention, as follows:
1. get coated vitamin C (content is more than 95%), standby.
2. get the above glucose of 80 orders (purity is greater than 93%), standby.
3. get ammonia chloride (NH
4Cl) 0.75 gram, sodium bicarbonate (NaHCO
3) 1 gram, dipotassium hydrogen phosphate (K
2HPO
4) 0.5 gram, sodium-acetate (CH
3COONa) 1.75 grams, magnesium chloride (MgCl
2) 0.4 gram, sodium-chlor (NaCl) 2 grams, glucose (C
6H
12O
6) 10 grams, add distilled water, add to 1000 milliliters, it is standby to make nutrient solution.
4. extracting lactic acid bacterium (Lactobacterium acidophilum Lactobacillus acidophilus) bacterial classification content is 5 * 10
8/ ml, yeast (brewer's yeast Saccharomyces cerevisiae) bacterial classification content is 20 * 10
8/ ml, filamentous fungus (unwrapping wire aspergillus Asporgillus cellulosae) bacterial classification content is 5 * 10
6/ ml carried out composite, standby according to 25: 10: 5.
5. get 4. bacterial classification and 3. nutrient solution pour in the fermentor tank in 1: 4 ratio, keep pH4.0-5.0, temperature 23-28 ℃, anaerobically fermenting was cultivated 4-10 days, waited to expect to send slightly sour-sweet strong distiller's yeast fragrance, showed and ferment successfully that it is standby to make bacterium liquid.
6. the bacterium liquid that 5. step is made makes the composite bacteria lyophilized powder by low temperature, freezing, drying, and is standby.
7. get lay-by material and 2. and 1. and 6. can make emergency strain in composite the mixing of 32: 16: 2 ratio.
The present invention has strong operability, with low cost, effect is remarkable, to the water surrounding non-secondary pollution, aquatic animal is had no side effect, aquaculture water quality there are advantages such as obvious repairing effect, be to comply with current social back to nature, treasure water and life, pursue green food, the development healthy aquaculture, the only way of realizing healthy consumption.
Claims (1)
- The preparation method of aquatic products probiotics emergency strain of the present invention, as follows:1. get coated vitamin C (content is more than 95%), standby.2. get the above glucose of 80 orders (purity is greater than 93%), standby.3. get ammonia chloride (NH 4Cl) 0.75 gram, sodium bicarbonate (NaHCO 3) 1 gram, dipotassium hydrogen phosphate (K 2HPO 4) 0.5 gram, sodium-acetate (CH 3COONa) 1.75 grams, magnesium chloride (MgCl 2) 0.4 gram, sodium-chlor (NaCl) 2 grams, glucose (C 6H 12O 6) 10 grams, add distilled water, add to 1000 milliliters, it is standby to make nutrient solution.4. extracting lactic acid bacterium (Lactobacterium acidophilum Lactobacillus acidophilus) bacterial classification content is 5 * 10 8/ ml, yeast (brewer's yeast Saccharomyces cerevisiae) bacterial classification content is 20 * 10 8/ ml, filamentous fungus (unwrapping wire aspergillus Asporgillus cellulosae) bacterial classification content is 5 * 10 6/ ml carried out composite, standby according to 25: 10: 5.5. get 4. bacterial classification and 3. nutrient solution pour in the fermentor tank in 1: 4 ratio, keep pH4.0-5.0, temperature 23-28 ℃, anaerobically fermenting was cultivated 4-10 days, waited to expect to send slightly sour-sweet strong distiller's yeast fragrance, showed and ferment successfully that it is standby to make bacterium liquid.6. the bacterium liquid that 5. step is made makes the composite bacteria lyophilized powder by low temperature, freezing, drying, and is standby.7. get lay-by material and 2. and 1. and 6. can make emergency strain in composite the mixing of 32: 16: 2 ratio.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100427793A CN101333502A (en) | 2007-06-27 | 2007-06-27 | Process for preparing emergency strain and repairing aquaculture environment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100427793A CN101333502A (en) | 2007-06-27 | 2007-06-27 | Process for preparing emergency strain and repairing aquaculture environment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101333502A true CN101333502A (en) | 2008-12-31 |
Family
ID=40196384
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100427793A Pending CN101333502A (en) | 2007-06-27 | 2007-06-27 | Process for preparing emergency strain and repairing aquaculture environment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101333502A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101536736B (en) * | 2009-04-25 | 2012-06-13 | 陈家珍 | Special fishing fertilizer for aquaculture and preparation method thereof |
| CN104232497A (en) * | 2013-06-19 | 2014-12-24 | 上海泓宝绿色水产科技发展有限公司 | EM flora preparation for improving water quality by compounding a plurality of strains and preparation method of EM flora preparation |
| CN104222490A (en) * | 2013-06-19 | 2014-12-24 | 上海泓宝绿色水产科技发展有限公司 | Compound feed obtained by fermenting natural cereals with probiotics and preparation method of compound feed |
| CN104755090A (en) * | 2012-04-05 | 2015-07-01 | 伦敦健康科学中心研究公司 | Food grade bacteria for the removal of toxic compounds |
| CN110713944A (en) * | 2018-07-12 | 2020-01-21 | 无锡三智生物科技有限公司 | Deodorizing microbial preparation for breeding house and manufacturing process |
| CN112280711A (en) * | 2020-11-02 | 2021-01-29 | 无锡泓宝微生物科技有限公司 | Water-regulating lovers for regulating and controlling water quality and preparation method thereof |
| CN115316318A (en) * | 2022-08-25 | 2022-11-11 | 承江 | Method for microecologically culturing leeches |
-
2007
- 2007-06-27 CN CNA2007100427793A patent/CN101333502A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101536736B (en) * | 2009-04-25 | 2012-06-13 | 陈家珍 | Special fishing fertilizer for aquaculture and preparation method thereof |
| CN104755090A (en) * | 2012-04-05 | 2015-07-01 | 伦敦健康科学中心研究公司 | Food grade bacteria for the removal of toxic compounds |
| CN104755090B (en) * | 2012-04-05 | 2020-10-20 | 伦敦健康科学中心研究公司 | Food grade bacteria for removal of toxic compounds |
| CN104232497A (en) * | 2013-06-19 | 2014-12-24 | 上海泓宝绿色水产科技发展有限公司 | EM flora preparation for improving water quality by compounding a plurality of strains and preparation method of EM flora preparation |
| CN104222490A (en) * | 2013-06-19 | 2014-12-24 | 上海泓宝绿色水产科技发展有限公司 | Compound feed obtained by fermenting natural cereals with probiotics and preparation method of compound feed |
| CN104232497B (en) * | 2013-06-19 | 2019-01-15 | 上海泓宝绿色水产股份有限公司 | A kind of compound EM flora preparation and preparation method thereof for improving water quality of multi-cultur es |
| CN110713944A (en) * | 2018-07-12 | 2020-01-21 | 无锡三智生物科技有限公司 | Deodorizing microbial preparation for breeding house and manufacturing process |
| CN112280711A (en) * | 2020-11-02 | 2021-01-29 | 无锡泓宝微生物科技有限公司 | Water-regulating lovers for regulating and controlling water quality and preparation method thereof |
| CN115316318A (en) * | 2022-08-25 | 2022-11-11 | 承江 | Method for microecologically culturing leeches |
| CN115316318B (en) * | 2022-08-25 | 2024-02-27 | 承江 | Method for microecological cultivation of leeches |
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Open date: 20081231 |