CN112280711A - Water-regulating lovers for regulating and controlling water quality and preparation method thereof - Google Patents
Water-regulating lovers for regulating and controlling water quality and preparation method thereof Download PDFInfo
- Publication number
- CN112280711A CN112280711A CN202011203115.2A CN202011203115A CN112280711A CN 112280711 A CN112280711 A CN 112280711A CN 202011203115 A CN202011203115 A CN 202011203115A CN 112280711 A CN112280711 A CN 112280711A
- Authority
- CN
- China
- Prior art keywords
- water
- regulating
- couple
- bacteria
- strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/306—Pesticides
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Abstract
The invention discloses a water-adjusting couple for regulating and controlling water quality, which comprises 50% of organic carbon source, 25% of composite flora, 11.5% of bacteria normal saline, 0.5% of dipotassium hydrogen phosphate, 0.5% of magnesium sulfate heptahydrate, 2.5% of sodium chloride, 2.5% of ammonia chloride, 5% of sodium acetate and 2.5% of sodium bicarbonate, wherein the composite flora comprises photosynthetic bacteria, lactic acid bacteria, saccharomycetes, actinomycetes and filamentous bacteria. The method has the advantages of efficiently decomposing residual baits, feces, animal and plant corpses and organic debris in the water body, reducing the organic oxygen demand (BOD) index of the water body, removing stress oxygen barrier of aquatic animals, regulating and controlling water quality in rainy days or nighttime pond low-oxygen environment, and avoiding the floating head problem of fishes and shrimps.
Description
Technical Field
The invention relates to a composite water-regulating microbial strain preparation, in particular to a water-regulating lover for regulating and controlling water quality and a preparation method thereof.
Background
The water quality of the aquaculture water is deteriorated due to the metabolism of fishes and shrimps, the putrefaction and deterioration of the residual baits eaten by the fishes and the shrimps and the growth and propagation of harmful algae such as blue algae, dinoflagellate and the like, so that the pH value of the water quality of the aquaculture water is reduced, and the dissolved oxygen of the aquaculture water is reduced. Especially in hot high-temperature seasons and when the culture density of the water body is high, the method is more obvious. The light fish and shrimp growth is influenced, and the yield of the fish and shrimp is reduced; serious people cause the fish and the shrimp to be 'over-pond', which can lead the fish and the shrimp to die in large batch and cause serious economic loss for farmers.
The invention is specially aimed at the aquatic product culture environment, and purposefully selects a multi-strain water-conditioning couple for improving water quality, and can rapidly and thoroughly degrade harmful amino nitrogen and nitroso nitrogen in water and eliminate harmful substances such as hydrogen sulfide and the like; the residual bait and excrement in water are efficiently decomposed, underwater sludge is eliminated, the organic oxygen demand (BOD) index of a water body is reduced, stress oxygen barrier of aquatic animals is eliminated, and water quality can be regulated and controlled in rainy days or nighttime pond hypoxia environment; the quantity of probiotics in water can be kept, and the colonization and invasion of other pathogenic bacteria can be inhibited; degrading organic matters, effectively adsorbing harmful substances, reducing the acidity of the water body, stabilizing the pH value of the water body, supplementing trace elements, improving the transparency of the water body and purifying the water quality.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a water-mixing lover for regulating and controlling water quality, which has the effects of stable structure and complete functions.
In order to achieve the technical purpose, the invention provides the following technical scheme: a water-mixing couple for regulating and controlling water quality comprises 50% of organic carbon source, 25% of compound flora, 11.5% of bacteria normal saline, 0.5% of dipotassium hydrogen phosphate, 0.5% of magnesium sulfate heptahydrate, 2.5% of sodium chloride, 2.5% of ammonia chloride, 5% of sodium acetate and 2.5% of sodium bicarbonate, wherein the compound flora comprises photosynthetic bacteria, lactic acid bacteria, saccharomycetes, actinomycetes and filamentous bacteria.
Preferably, the photosynthetic bacteria adopt rhodopseudomonas palustris, the lactic acid bacteria adopt lactobacillus acidophilus, the yeast bacteria adopt saccharomyces cerevisiae, the actinomycetes adopt aspergillus actinomycete, the filamentous bacteria adopt polysporum, the total bacteria concentration of the composite bacteria is more than 30 x 10^8cfu/ml, and the content of the rhodopseudomonas palustris is more than or equal to 60 percent.
Aiming at the defects in the prior art, the invention also aims to provide a strain propagation method for a water-regulating lovers for regulating and controlling water quality.
In order to achieve the technical purpose, the invention provides the following technical scheme: a method for expanding culture of strains of a water-mixing lovers for regulating and controlling water quality. The method comprises the following steps:
s1, preparing a culture medium, namely fully dissolving bacteria normal saline, dipotassium phosphate, magnesium sulfate heptahydrate, sodium chloride, ammonia chloride, sodium acetate, sodium bicarbonate and distilled water;
s2, preparing a water-mixing lover strain, wherein rhodopseudomonas palustris in photosynthetic bacteria is taken, and the content of the strain is 30 multiplied by 10^8cfu/ml or more; the lactobacillus acidophilus in the lactobacillus has the strain content of 5 multiplied by 10^8cfu/ml or more; the content of the strains in the saccharomyces cerevisiae in the saccharomycetes is 20 multiplied by 10^8cfu/ml or more; the content of actinomycete species in the filamentous fungi is 5 multiplied by 10^6cfu/ml or more; the multi-spore spring spawn is grown, the strain content is 60 multiplied by 10^8cfu/ml or more; taking 8-12 parts of rhodopseudomonas palustris, 4-6 parts of lactobacillus acidophilus, 1-3 parts of brewing yeast, 1-2 parts of actinomycete aspergillus and 1-3 parts of polyspora spring spawn to prepare strains for later use;
s3, preparing a composite bacterium mother solution, namely adding 5 kg of a water-adjusting couple strain, 5 kg of a water-adjusting couple culture medium, 10 kg of a water-adjusting couple carbon source and 480 kg of water for mixed culture, firstly putting 5 kg of the water-adjusting couple strain, 5 kg of the water-adjusting couple culture medium and 10 kg of the water-adjusting couple carbon source into a transparent or semitransparent sealed clean plastic container, then adding 480 kg of water for uniformly stirring, sealing and expanding culture, irradiating by sunlight in daytime, and after 5-7 days of expanding culture, allowing the liquid surface to generate foam and emit sweet and sour fragrance, indicating that the expanding culture is successful, and being capable of being used.
Preferably, in step S3, the water is well water or cold boiled water.
Preferably, in step S3, if the plastic container is inflated too much, the cap can be unscrewed properly and then deflated.
Preferably, the 3000ml of the bacterial liquid after the water regulating lovers are expanded and cultured is directly sprinkled on the water surface per mu once every 3 to 5 days, and the sprinkling is doubled in high-temperature seasons or when the water quality is deteriorated.
Preferably, 3000-5000ml of the bacteria liquid expanded and cultured by the water-regulating lovers is directly sprinkled on the water body per mu of water surface.
Preferably, 5000ml of the bacteria liquid expanded and cultured by the water-regulating lovers is added with 5000ml of cold boiled water and 25 kg of dry excrement per mu of water surface, and the mixture is fermented and then is put into the water body.
Preferably, the water-mixing couples can be fermented in the aquaculture field, expanded culture in the field and directly used in the field.
In summary, the present invention achieves the following effects:
1. the method has the advantages that residual baits, feces, animal and plant corpses and organic debris in the water body are efficiently decomposed, the organic oxygen demand (BOD) index of the water body is reduced, the stress oxygen barrier of aquatic animals is eliminated, the water quality can be regulated and controlled in rainy days or in a low-oxygen environment of a pond at night, and the floating head problem of fishes and shrimps is avoided;
2. eliminating harmful substances such as amino nitrogen, nitroso nitrogen, hydrogen sulfide and the like in water, converting the harmful substances into a nutrient source of unicellular algae, promoting the growth of excellent algae and quickly culturing plankton baits;
3. inhibiting the planting and invasion of harmful bacteria in the water body and eliminating toxic factors;
4. heavy metals, pesticides, algal toxins and chemical industrial pollution are synergistically cleared up, and the Chemical Oxygen Demand (COD) index of the water body is reduced;
5. the compound feed can maintain the micro-ecological balance in the bodies of the aquatic animals, promote the digestion and absorption of the aquatic animals on nutrient substances and improve the utilization rate of the feed;
6. stabilizing the pH value of the water body, keeping the population quantity of beneficial microorganisms and algae in the water body, and inhibiting the growth of harmful algae such as ootheca, microcystis and the like.
Detailed Description
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Example (b): a water-mixing couple for regulating and controlling water quality comprises 50% of organic carbon source, 25% of compound flora, 11.5% of bacteria normal saline, 0.5% of dipotassium hydrogen phosphate, 0.5% of magnesium sulfate heptahydrate, 2.5% of sodium chloride, 2.5% of ammonia chloride, 5% of sodium acetate and 2.5% of sodium bicarbonate, wherein the compound flora comprises photosynthetic bacteria, lactic acid bacteria, yeasts, actinomycetes and filamentous bacteria.
The organic carbon source may be a saccharide, a fatty acid or petroleum. The photosynthetic bacteria adopt rhodopseudomonas palustris, the lactic acid bacteria adopt lactobacillus acidophilus, the microzyme adopts saccharomyces cerevisiae, the actinomycetes adopt actinomycete aspergillus, the filamentous bacteria adopt multi-spongenous bacteria, the total bacteria concentration of the composite bacteria is more than 30 multiplied by 10^8cfu/ml, and the content of the rhodopseudomonas palustris is more than or equal to 60 percent.
A method for expanding culture of strains of a water-mixing lovers for regulating and controlling water quality. The method comprises the following steps:
s1, preparing a culture medium, namely fully dissolving bacteria normal saline, dipotassium phosphate, magnesium sulfate heptahydrate, sodium chloride, ammonia chloride, sodium acetate, sodium bicarbonate and distilled water;
s2, preparing a water-mixing lover strain, wherein rhodopseudomonas palustris in photosynthetic bacteria is taken, and the content of the strain is 30 multiplied by 10^8cfu/ml or more; the lactobacillus acidophilus in the lactobacillus has the strain content of 5 multiplied by 10^8cfu/ml or more; the content of the strains in the saccharomyces cerevisiae in the saccharomycetes is 20 multiplied by 10^8cfu/ml or more; the content of actinomycete species in the filamentous fungi is 5 multiplied by 10^6cfu/ml or more; the multi-spore spring spawn is grown, the strain content is 60 multiplied by 10^8cfu/ml or more; taking 8-12 parts of rhodopseudomonas palustris, 4-6 parts of lactobacillus acidophilus, 1-3 parts of brewing yeast, 1-2 parts of actinomycete aspergillus and 1-3 parts of polyspora spring spawn to prepare strains for later use;
s3, preparing a composite bacterium mother solution, namely adding 5 kg of a water-adjusting couple strain, 5 kg of a water-adjusting couple culture medium, 10 kg of a water-adjusting couple carbon source and 480 kg of water for mixed culture, firstly putting 5 kg of the water-adjusting couple strain, 5 kg of the water-adjusting couple culture medium and 10 kg of the water-adjusting couple carbon source into a transparent or semitransparent sealed clean plastic container, then adding 480 kg of water for uniformly stirring, sealing and expanding culture, irradiating by sunlight in daytime, and after 5-7 days of expanding culture, allowing the liquid surface to generate foam and emit sweet and sour fragrance, indicating that the expanding culture is successful, and being capable of being used.
In step S3, well water or cold boiled water is used as water. If the plastic container is seriously inflated, the cover can be properly unscrewed and deflated, and then the plastic container is screwed down.
Physical and chemical properties: a pale red liquid.
The application range is as follows: is suitable for all the breeding ponds and culture ponds for marine and freshwater aquatic animals.
The dosage is as follows:
1. adjusting the water quality: 1500-3000ml of water-adjusting couple expanded culture liquid is directly sprinkled (sprinkled once every 3-5 days) on the water surface (the water depth is 1 m) per mu, and can be sprinkled twice in high-temperature seasons or when the water quality is deteriorated.
2. Treating fertilizer water: 3000 plus 5000ml of water-regulating couple expanded-culture liquid is directly sprinkled on the water body per mu of water surface (the water depth is 1 m), or 5000ml of water-regulating couple expanded-culture liquid is mixed with 5000ml of cold boiled water and 25 kg of dry excrement (various livestock and poultry excrement) and then the mixture is thrown into the water body after mixed fermentation.
Note that:
1. the 'water-mixing lovers expanding culture liquid' is not influenced by weather, illumination and water temperature, and has better using effect by splashing water before strong wind and rainstorm or at night;
2. chemical medicines and antibiotic medicines with the bactericidal effect are forbidden to be used within three days before and five days after the 'water-mixing lover expanding culture solution' is put in;
3. the bottom of the 'water-adjusting couple expanding bacteria liquid' is normal with a little sediment or a little foam floating, and before use, the bacteria liquid in the barrel is firstly stirred evenly and then used;
4. the 'water-mixing couple expanding culture solution' is prepared immediately, if the residual is preserved in a sealed way, the smell is not acid or rotten, which indicates that the bacterium is deteriorated, and the bacterium is not reused;
5. the product is safe to use, and has better using effect when being matched with Hongbao detoxification liquid or other Hongbao series products.
6. After three days, the water can be changed at any time.
Packaging: plastic bucket set, 5000 ml/bucket, 4 buckets/set (box).
And (3) storage: sealing and shading, and storing in shade, ventilated and dry place.
A retention period: for 2 years.
The report on the application of the water-regulating lovers in the culture of penaeus vannamei stated in this example is as follows:
treating a water body: two adjacent south America white shrimp ponds with 25 mu of water surface in the new coastal area of Shaoxing Shangyu area of Zhejiang are taken for carrying out a contrast test, the water depth is about 1 m, the ponds are in the south-north direction, are rectangular, and are convenient to drain and irrigate. The shrimp larvae are put in for 4 months and 28 days, the putting amount is 4 ten thousand tails/mu, the shrimp larvae are divided into an experimental group and a control group, the treatment period is 2 months, the shrimp larvae are used for 1 time every 10 days in the first 20 days, and 1500ml of the water conditioning lover preparation is used on the water surface of each mu; the product is applied 1 time every 8 days after 40 days, and 3000ml per mu of water surface. During the experiment period, the control group and the experimental group are fed with the feed twice a day, the control group and the experimental group are fed once at 9:00 am and once at 17:00 evening, and other feeding conditions are the same. Measuring pond water quality indexes of a control group and an experimental group 20 days after seedling placement, measuring the pond water quality indexes once every 5 days and measuring the pond water quality indexes for 6 times in succession, and calculating an average value (see the following table):
by observing the whole experimental process, we conclude that:
the growth speed of the penaeus vannamei boone is obviously accelerated
In the test period, the growth speed of the test group of the penaeus vannamei boone is obviously accelerated compared with that of the control group of the penaeus vannamei boone at the same period; the final average weight of the experimental group of the penaeus vannamei boone is increased by about 23.29 percent compared with the average weight of the control group of the penaeus vannamei boone.
(II) the incidence of the penaeus vannamei boone is obviously reduced
The morbidity of the penaeus vannamei after the experiment group is fed with the water-adjusting lovers is reduced by about 50 percent compared with that of the control group.
And thirdly, the water quality is obviously improved, the mu cost is greatly reduced, and the economic benefit is indirectly improved.
After the experiment group uses the water-mixing lovers, the water quality is obviously improved, harmful substances such as ammonia nitrogen, nitrite and hydrogen sulfide in the pond are obviously reduced, and the pH value is always stable between 7.5 and 8.7; the ammonia nitrogen in the water body is stable and can be controlled within a safe range. Meanwhile, the dissolved oxygen in the water body can be indirectly improved, so that the water quality is fresh. The transparency of the water body is kept about 25-40 cm all the time. Compared with a control group, the water change amount is reduced by 50% in the whole culture process.
The experimental group of the penaeus vannamei boone culture process always insists on using microbial agents without using chemical drugs or antibiotics, so that the quality and the taste of the cultured penaeus vannamei boone are better than those of the penaeus vannamei boone cultured in a common pond, and the point is proved in market information feedback.
Claims (9)
1. A water-mixing lover for regulating and controlling water quality is characterized in that: the microbial composite comprises 50% of organic carbon source, 25% of composite flora, 11.5% of bacteria physiological saline, 0.5% of dipotassium hydrogen phosphate, 0.5% of magnesium sulfate heptahydrate, 2.5% of sodium chloride, 2.5% of ammonia chloride, 5% of sodium acetate and 2.5% of sodium bicarbonate, wherein the composite flora comprises photosynthetic bacteria, lactic acid bacteria, yeasts, actinomycetes and filamentous bacteria.
2. A water-mixing couple for regulating water quality according to claim 1, wherein: the photosynthetic bacteria adopt rhodopseudomonas palustris, the lactic acid bacteria adopt lactobacillus acidophilus, the yeast bacteria adopt saccharomyces cerevisiae, the actinomycetes adopt aspergillus actinomycete, the filamentous bacteria adopt multi-spongening fungi, the total bacteria concentration of the composite bacteria is more than 30 multiplied by 10^8cfu/ml, and the content of the rhodopseudomonas palustris is more than or equal to 60 percent.
3. A strain propagation method for the water-mixing couples or couples used for regulating and controlling water quality according to claim 1 or 2, comprising the following steps:
s1, preparing a culture medium, namely fully dissolving 23% of bacterial normal saline, 1% of dipotassium hydrogen phosphate, 1% of magnesium sulfate heptahydrate, 5% of sodium chloride, 5% of ammonia chloride, 10% of sodium acetate, 5% of sodium bicarbonate and 50% of distilled water;
s2, preparing a water-mixing lover strain, wherein rhodopseudomonas palustris in photosynthetic bacteria is taken, and the content of the strain is 30 multiplied by 10^8cfu/ml or more; the lactobacillus acidophilus in the lactobacillus has the strain content of 5 multiplied by 10^8cfu/ml or more; the content of the strains in the saccharomyces cerevisiae in the saccharomycetes is 20 multiplied by 10^8cfu/ml or more; the content of actinomycete species in the filamentous fungi is 5 multiplied by 10^6cfu/ml or more; the multi-spore spring spawn is grown, the strain content is 60 multiplied by 10^8cfu/ml or more; taking 8-12 parts of rhodopseudomonas palustris, 4-6 parts of lactobacillus acidophilus, 1-3 parts of brewing yeast, 1-2 parts of actinomycete aspergillus and 1-3 parts of polyspora spring spawn to prepare strains for later use;
s3, preparing a composite bacterium mother solution, namely adding 5 kg of a water-adjusting couple strain, 5 kg of a water-adjusting couple culture medium, 10 kg of a water-adjusting couple carbon source and 480 kg of water for mixed culture, firstly putting 5 kg of the water-adjusting couple strain, 5 kg of the water-adjusting couple culture medium and 10 kg of the water-adjusting couple carbon source into a transparent or semitransparent sealed clean plastic container, then adding 480 kg of water for uniformly stirring, sealing and expanding culture, irradiating by sunlight in daytime, and after 5-7 days of expanding culture, allowing the liquid surface to generate foam and emit sweet and sour fragrance, indicating that the expanding culture is successful, and being capable of being used.
4. The preparation method of a water-regulating couple according to claim 3, wherein the water-regulating couple is characterized in that: in the step S3, well water or cold boiled water is used as the water.
5. The preparation method of a water-regulating couple according to claim 3, wherein the water-regulating couple is characterized in that: in step S3, if the plastic container is inflated too much, the cap can be unscrewed properly and then deflated.
6. The preparation method of a water-regulating couple according to claim 3, wherein the water-regulating couple is characterized in that: the bacterial liquid 1500-3000ml after the water regulating lovers are used for expanding culture is directly sprinkled on the water surface of each mu, the bacterial liquid is sprinkled once every 3-5 days, and the bacterial liquid is sprinkled twice in high-temperature seasons or when the water quality is deteriorated.
7. The preparation method of a water-regulating couple according to claim 3, wherein the water-regulating couple is characterized in that: 3000ml of the bacteria liquid expanded and cultured by the water-regulating couples and 5000ml of the bacteria liquid are directly splashed on the water body per mu.
8. The preparation method of a water-regulating couple according to claim 3, wherein the water-regulating couple is characterized in that: 5000ml of the bacteria liquid expanded and cultured by the water-regulating couples is added into 5000ml of cold boiled water and 25 kg of dry excrement per mu of water surface, mixed and fermented, and then the mixture is thrown into the water body.
9. A water-mixing couple for regulating water quality according to claim 1, wherein: the water-mixing lovers can be fermented in the aquaculture field, expanded in the field and directly used in the field.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011203115.2A CN112280711A (en) | 2020-11-02 | 2020-11-02 | Water-regulating lovers for regulating and controlling water quality and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011203115.2A CN112280711A (en) | 2020-11-02 | 2020-11-02 | Water-regulating lovers for regulating and controlling water quality and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112280711A true CN112280711A (en) | 2021-01-29 |
Family
ID=74352759
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011203115.2A Pending CN112280711A (en) | 2020-11-02 | 2020-11-02 | Water-regulating lovers for regulating and controlling water quality and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112280711A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101082028A (en) * | 2006-06-01 | 2007-12-05 | 上海泓宝绿色水产科技发展有限公司 | Preparation of water-adjusting bacterium and method for restoring aquaculture environment |
| CN101333502A (en) * | 2007-06-27 | 2008-12-31 | 上海泓宝绿色水产科技发展有限公司 | Process for preparing emergency strain and repairing aquaculture environment |
| CN103641229A (en) * | 2013-11-14 | 2014-03-19 | 钟国防 | Method for regulating and controlling of water quality of Litopenaeus Vannamei culture pond |
| CN104232497A (en) * | 2013-06-19 | 2014-12-24 | 上海泓宝绿色水产科技发展有限公司 | EM flora preparation for improving water quality by compounding a plurality of strains and preparation method of EM flora preparation |
| CN109182157A (en) * | 2018-09-17 | 2019-01-11 | 无锡跃洋生物科技有限公司 | A kind of composite bacteria preparation and preparation method thereof improving breeding environment |
-
2020
- 2020-11-02 CN CN202011203115.2A patent/CN112280711A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101082028A (en) * | 2006-06-01 | 2007-12-05 | 上海泓宝绿色水产科技发展有限公司 | Preparation of water-adjusting bacterium and method for restoring aquaculture environment |
| CN101333502A (en) * | 2007-06-27 | 2008-12-31 | 上海泓宝绿色水产科技发展有限公司 | Process for preparing emergency strain and repairing aquaculture environment |
| CN104232497A (en) * | 2013-06-19 | 2014-12-24 | 上海泓宝绿色水产科技发展有限公司 | EM flora preparation for improving water quality by compounding a plurality of strains and preparation method of EM flora preparation |
| CN103641229A (en) * | 2013-11-14 | 2014-03-19 | 钟国防 | Method for regulating and controlling of water quality of Litopenaeus Vannamei culture pond |
| CN109182157A (en) * | 2018-09-17 | 2019-01-11 | 无锡跃洋生物科技有限公司 | A kind of composite bacteria preparation and preparation method thereof improving breeding environment |
Non-Patent Citations (1)
| Title |
|---|
| 刘俊新等: "铁细菌在污水除磷中的应用研究", 《环境科技》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20190045756A1 (en) | Dark-bright integrated greenhouse system in intensive recirculating eco-aquaculture and aquaculture method | |
| US8133391B2 (en) | Method of ecological restoration of water bodies containing excess nutrient | |
| CN101376877B (en) | Compound microecological preparation for purifying cultivation water, preparation formulation thereof and preparation processes of the preparation | |
| CN101575144B (en) | Method for repairing water ecology | |
| CN102047857A (en) | Novel high-efficiency biological fish manure | |
| CN1500749A (en) | Modifying agent for microorganism breeding water and preparing method thereof | |
| CN102134558B (en) | Bacillussubtilis and application thereof in raising Apostichopus japonicus | |
| CN103992187A (en) | Biological bacterial fertilizer for preventing and treating moss in water body and preparation method thereof | |
| CN106035171A (en) | Prawn ecological culturing method based on microbial regulation | |
| CN106380004B (en) | Aquaculture waters restoration of the ecosystem agent and preparation method thereof | |
| CN105130012A (en) | Water quality improver | |
| CN101082028A (en) | Preparation of water-adjusting bacterium and method for restoring aquaculture environment | |
| CN110050904A (en) | A kind of fulvic acid used for aquiculture and lactobacillus fermentation material and preparation method thereof | |
| CN104651282B (en) | A kind of preparation method of Composite Photosynthetic Bacteria preparation | |
| CN106967647B (en) | Pseudoalteromonas foli and application method thereof | |
| CN106854629A (en) | A kind of high concentration composite bacillus and its preparation and application | |
| CN105309388B (en) | A kind of daphnia heatproof acclimation method and the method for carrying out restoration of the ecosystem to water body using daphnia | |
| CN111109176B (en) | Three-stage water purification system for aquaculture | |
| CN102919183B (en) | Method for effectively inhibiting vibrio breeding in shrimp culture | |
| CN110250047A (en) | A kind of method of industrial aquaculture threadfin | |
| CN107361010B (en) | Coastal saline-alkali soil-based water quality regulation and control method for yellow river mouth hairy crab culture pond | |
| Dewi et al. | Water quality in the maintenance of oreochromis niloticus through environmentally friendly biofloc technology | |
| CN112280711A (en) | Water-regulating lovers for regulating and controlling water quality and preparation method thereof | |
| CN107858307A (en) | A kind of gemma bacterium powder and preparation method thereof | |
| CN1580002A (en) | Method for preparing rich water compound bacteria and repairing aquaculture envionment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210129 |