CN107858307A - A kind of gemma bacterium powder and preparation method thereof - Google Patents

A kind of gemma bacterium powder and preparation method thereof Download PDF

Info

Publication number
CN107858307A
CN107858307A CN201711225355.0A CN201711225355A CN107858307A CN 107858307 A CN107858307 A CN 107858307A CN 201711225355 A CN201711225355 A CN 201711225355A CN 107858307 A CN107858307 A CN 107858307A
Authority
CN
China
Prior art keywords
bacterium powder
bacillus
gemma bacterium
vitamin
gemma
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201711225355.0A
Other languages
Chinese (zh)
Inventor
郑宝权
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenzhen Tian Tian International Bioengineering Technology Co Ltd
Original Assignee
Shenzhen Tian Tian International Bioengineering Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shenzhen Tian Tian International Bioengineering Technology Co Ltd filed Critical Shenzhen Tian Tian International Bioengineering Technology Co Ltd
Priority to CN201711225355.0A priority Critical patent/CN107858307A/en
Publication of CN107858307A publication Critical patent/CN107858307A/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/341Consortia of bacteria
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0065Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • C12N9/20Triglyceride splitting, e.g. by means of lipase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477Hemicellulases not provided in a preceding group
    • C12N9/2488Mannanases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y111/00Oxidoreductases acting on a peroxide as acceptor (1.11)
    • C12Y111/01Peroxidases (1.11.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/01Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003Triacylglycerol lipase (3.1.1.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/01Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01011Pectinesterase (3.1.1.11)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015Polygalacturonase (3.2.1.15)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/02Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02002Pectate lyase (4.2.2.2)
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20Nature of the water, waste water, sewage or sludge to be treated from animal husbandry

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention discloses a kind of gemma bacterium powder, is made up of by mass percentage following components:Bacillus subtilis 31.1~70%, bacillus licheniformis 20.8~50%, bacillus megaterium 4~16%, vitamin C 2~5%, high active enzyme 0.03~0.1%;The gemma bacterium powder that the present invention obtains can fast decoupled, conversion water in residual bait, excrement, mud, improve water body environment and increase dissolved oxygen, and the growth of various weeds can be controlled, prevent the growth of blue-green algae, Euglena, moss, silkweed, sponge algae, so that the harmful bacteria in water body is largely reduced, have the function that to purify and detoxify, so as to effectively improve water quality, prevent the deterioration and virosis, bacterial disease, the generation for stealing the disease such as dead disease of water body environment, make cultivation object few sick or not sick, be advantageous to cultivate the growth of object.

Description

A kind of gemma bacterium powder and preparation method thereof
Technical field
The present invention relates to green agriculture, livestock breeding industry and technical field of aquaculture, more particularly to one kind to be used for water Produce gemma bacterium powder of cultivation and preparation method thereof.
Background technology
At this stage for seawater, fresh water, fish, shrimp, crab, shellfish, freshwater mussel, pearl, jellyfish, sea cucumber, flounder, flounder, oyster, grouper, gold The aquaculture of the various aquatic organisms such as butterfish is that the production that aquatic animals and plants are bred, cultivated and harvested under manual control is lived It is dynamic, it is commonly included under artificial feeding management, the overall process of aquatic products is formed from seed, broadly may also comprise aquatic resources increasing Grow, aquaculture there are the modes such as extensive cultivation, intensive culture and high density culture;But because in daily breeding process because sinking to the feeding of bottom of pond Material, excreta, the dead remains of fertilizer and animals and plants are exceeded easily to cause change of water quality, anoxic in water, harmful substance increase, is harmful to Bacterium is increased, and toxin is larger, so as to cause various virosis to occur, is unfavorable for the growth of water body animals and plants.
The content of the invention
In view of this, it is a primary object of the present invention to provide a kind of gemma bacterium powder and preparation method thereof, solve existing Harmful bacteria is more in water body during aquaculture in technology, and toxin is big, causes the deterioration of water body environment and the hair of virosis It is raw, so as to be unfavorable for the growth of water body animals and plants and it is easily sick the problem of;Present invention also offers the preparation side of the gemma bacterium powder Method.
To reach above-mentioned purpose, the technical proposal of the invention is realized in this way:A kind of gemma bacterium powder, is pressed by following components Mass percent forms:Bacillus subtilis 31.1~70%, bacillus licheniformis 20.8~50%, bacillus megaterium 4~ 16%th, vitamin C 2~5%, high active enzyme 0.03~0.1%.
Preferably, the high active enzyme is protease, amylase, cellulase, lipase, pectase, mannonase One or more in peroxidase.
What another technical scheme of the present invention was realized in:A kind of preparation method of gemma bacterium powder, it is characterised in that This method is achieved by the steps of:
Step 1, following components is weighed respectively by mass percentage:Bacillus subtilis 31.1~70%, lichens gemma bar Bacterium 20.8~50%, bacillus megaterium 4~16%, vitamin C 2~5%, high active enzyme 0.03~0.1%;
Step 2, by the bacillus subtilis described in step 1, bacillus licheniformis, bacillus megaterium, vitamin C, High active enzyme is added in mixer and stirred, and is stood, and obtains the first mixed material;
Step 3, concentration is dried in the first mixed material obtained to the step 2, obtains gemma bacterium powder.
In the step 2, the time of the standing is 30-50min.
In the step 2, the temperature of the standing is 40~50 DEG C.
In the step 3, the time of the dry concentration is 20~50min.
Compared with prior art, the gemma bacterium powder that obtains of the present invention can fast decoupled, the residual bait in conversion water, excrement, Mud, improving water body environment simultaneously increases dissolved oxygen, and can control the growth of various weeds, prevent blue-green algae, Euglena, moss, Silkweed, the growth of sponge algae so that the harmful bacteria in water body is largely reduced, and is haveed the function that to purify and is detoxified, so as to Effectively improve water quality, it is therefore prevented that the deterioration of water body environment and virosis, bacterial disease, the generation for stealing the disease such as dead disease, allow cultivation Object is few sick or not sick, is advantageous to cultivate the growth of object.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to Limit the present invention.
The formulation of the present invention includes:Pulvis, aqua, paste and various liquid, we carry out detailed just for pulvis herein Thin description.
A kind of gemma bacterium powder provided by the invention, is made up of by mass percentage following components:Distinguish by mass percentage Weigh following components:Bacillus subtilis 31.1~70%, bacillus licheniformis 20.8~50%, bacillus megaterium 4~ 16%th, vitamin C 2~5%, high active enzyme 0.03~0.1%.
Wherein, high active enzyme is protease, amylase, cellulase, lipase, pectase, mannonase peroxidating One or more in thing enzyme.
A kind of preparation method of gemma bacterium powder provided by the invention, this method are achieved by the steps of:
Step 1, following components is weighed respectively by mass percentage:Bacillus subtilis 31.1~70%, lichens gemma bar Bacterium 20.8~50%, bacillus megaterium 4~16%, vitamin C 2~5%, high active enzyme 0.03~0.1%;
Step 2, by the bacillus subtilis described in step 1, bacillus licheniformis, bacillus megaterium, vitamin C, High active enzyme, which is added in mixer, stirs and is stood at 30~50 DEG C 30-50min, obtains the first mixed material;
Step 3,20~50min of concentration is dried in the first mixed material obtained to the step 2, obtains Bacillus Powder.
The gemma bacterium powder that the present invention obtains can fast decoupled, conversion water in residual bait, excrement, mud, improve water body ring Border simultaneously increases dissolved oxygen, and can control the growth of various weeds, prevents blue-green algae, Euglena, moss, silkweed, sponge algae Growth so that the harmful bacteria in water body is largely reduced, and is haveed the function that to purify and is detoxified, so as to effectively improving water Matter, it is therefore prevented that the deterioration of water body environment and virosis, bacterial disease, the generation for stealing the disease such as dead disease, make cultivation object few sick or not It is sick, be advantageous to cultivate the growth of object.
In addition, the gemma bacterium powder that the present invention obtains:1) have the function that to accelerate digestion, the nitrous that will can be harmful in water Acid, ammonia nitrogen, hydrogen sulfide etc. are converted into harmless nitrogen, formulation fertilizer containing nitrogen, are absorbed by algae;2) water body and fish can be increased Profitable strain in shrimp and crab body, long-term use can effectively prevent virus diseases, steal dead disease, bacterial disease, such as herpesviral, hemorrhage The generation of the disease such as poison, corpus hemorrhagicum virus, the mould fungi of the gill, effectively prevents the flourish of harmful bacteria virus, prevents vibriosis;3) energy Enough stable water PH value, release water body mycin, prevent it is dead steathily;Long-term use can prevent from drawing in the net bleeding body surface and redden, and increase resistance to fortune Movement Capabilities.
Embodiment 1
A kind of preparation method of gemma bacterium powder, this method are achieved by the steps of:
Step 1, following components is weighed respectively by mass percentage:Bacillus subtilis 37%, bacillus licheniformis 50%, Bacillus megaterium 9%, vitamin C 3.94%, high active enzyme 0.06%;
Step 2, by described in step 1:Bacillus subtilis, bacillus licheniformis, bacillus megaterium, vitamin C, High active enzyme, which is added in mixer, stirs and is stood at 30 DEG C insulation 40min, obtains the first mixed material;
Step 3, concentration 30min is dried in the first mixed material obtained to the step 2, obtains gemma bacterium powder.
Embodiment 2
A kind of preparation method of gemma bacterium powder, this method are achieved by the steps of:
Step 1, following components is weighed respectively by mass percentage:Bacillus subtilis 37%, bacillus licheniformis 50%, Bacillus megaterium 9%, vitamin C 3.94%, high active enzyme 0.06%;
Step 2, by described in step 1:Bacillus subtilis, bacillus licheniformis, bacillus megaterium, vitamin C, High active enzyme, which is added in mixer, to stir and insulation 30min is stood at lower 50 DEG C, obtains the first mixed material;
Step 3, concentration 20min is dried in the first mixed material obtained to the step 2, obtains gemma bacterium powder.
Embodiment 3
A kind of preparation method of gemma bacterium powder, this method are achieved by the steps of:
Step 1, following components is weighed respectively by mass percentage:Bacillus subtilis 37%, bacillus licheniformis 50%, Bacillus megaterium 9%, vitamin C 3.94%, high active enzyme 0.06%;
Step 2, by described in step 1:Bacillus subtilis, bacillus licheniformis, bacillus megaterium, vitamin C, High active enzyme, which is added in mixer, stirs and is stood at 40 DEG C insulation 50min, obtains the first mixed material;
Step 3, concentration 50min is dried in the first mixed material obtained to the step 2, obtains gemma bacterium powder.
Embodiment 4
A kind of preparation method of gemma bacterium powder, this method are achieved by the steps of:
Step 1, following components is weighed respectively by mass percentage:Bacillus subtilis 37%, bacillus licheniformis 50%, Bacillus megaterium 9%, vitamin C 3.94%, high active enzyme 0.06%;
Step 2, by described in step 1:Bacillus subtilis, bacillus licheniformis, bacillus megaterium, vitamin C, High active enzyme, which is added in mixer, stirs and is stood at 30 DEG C insulation 40min, obtains the first mixed material;
Step 3, concentration 30min is dried in the first mixed material obtained to the step 2, obtains gemma bacterium powder.
Embodiment 5
A kind of preparation method of gemma bacterium powder, this method are achieved by the steps of:
Step 1, following components is weighed respectively by mass percentage:Bacillus subtilis 70%, bacillus licheniformis 20.8%th, bacillus megaterium 5.5%, vitamin C 3.67%, high active enzyme 0.03%;
Step 2, by described in step 1:Bacillus subtilis, bacillus licheniformis, bacillus megaterium, vitamin C, High active enzyme, which is added in mixer, to stir and insulation 30min is stood at lower 50 DEG C, obtains the first mixed material;
Step 3, concentration 20min is dried in the first mixed material obtained to the step 2, obtains gemma bacterium powder.
Embodiment 6
A kind of preparation method of gemma bacterium powder, this method are achieved by the steps of:
Step 1, following components is weighed respectively by mass percentage:Bacillus subtilis 70%, bacillus licheniformis 20.8%th, bacillus megaterium 5.5%, vitamin C 3.67%, high active enzyme 0.03%;
Step 2, by described in step 1:Bacillus subtilis, bacillus licheniformis, bacillus megaterium, vitamin C, High active enzyme, which is added in mixer, stirs and is stood at 40 DEG C insulation 50min, obtains the first mixed material;
Step 3, concentration 50min is dried in the first mixed material obtained to the step 2, obtains gemma bacterium powder.
Embodiment 7
A kind of preparation method of gemma bacterium powder, this method are achieved by the steps of:
Step 1, following components is weighed respectively by mass percentage:Bacillus subtilis 52%, bacillus licheniformis 38%, Bacillus megaterium 7%, vitamin C 2.9%, high active enzyme 0.1%;
Step 2, by described in step 1:Bacillus subtilis, bacillus licheniformis, bacillus megaterium, vitamin C, High active enzyme, which is added in mixer, stirs and is stood at 30 DEG C insulation 40min, obtains the first mixed material;
Step 3, concentration 30min is dried in the first mixed material obtained to the step 2, obtains gemma bacterium powder.
Embodiment 8
A kind of preparation method of gemma bacterium powder, this method are achieved by the steps of:
Step 1, following components is weighed respectively by mass percentage:Bacillus subtilis 52%, bacillus licheniformis 38%, Bacillus megaterium 7%, vitamin C 2.9%, high active enzyme 0.1%;
Step 2, by described in step 1:Bacillus subtilis, bacillus licheniformis, bacillus megaterium, vitamin C, High active enzyme, which is added in mixer, to stir and insulation 30min is stood at lower 50 DEG C, obtains the first mixed material;
Step 3, concentration 20min is dried in the first mixed material obtained to the step 2, obtains gemma bacterium powder.
Embodiment 9
A kind of preparation method of gemma bacterium powder, this method are achieved by the steps of:
Step 1, following components is weighed respectively by mass percentage:Bacillus subtilis 52%, bacillus licheniformis 38%, Bacillus megaterium 7%, vitamin C 2.9%, high active enzyme 0.1%;
Step 2, by described in step 1:Bacillus subtilis, bacillus licheniformis, bacillus megaterium, vitamin C, High active enzyme, which is added in mixer, stirs and is stood at 40 DEG C insulation 50min, obtains the first mixed material;
Step 3, concentration 50min is dried in the first mixed material obtained to the step 2, obtains gemma bacterium powder.
Experimental example:
Gemma bacterium powder needs full pool spilling head after being soaked 2 hours by water when in use, while starts aerator, and mu is used The 20-30 grams of mu rice depth of waters are measured, regular 7-10 days using once.
The gemma bacterium powder that embodiment 1 to embodiment 9 obtains is detected and observed respectively the growth feelings of water body animals and plants Condition, result of the test such as following table:
The present invention of table 1 obtains detection correction data of the gemma bacterium powder to the water body animals and plants incidence of disease
The viral incidence of disease The virosis incidence of disease/% The bacterial disease incidence of disease/% The dead sick incidence of disease/% steathily
Embodiment 1 1.0% 2.3% 3.3%
Embodiment 2 1.5% 3.1% 3.2%
Embodiment 3 1.2% 2.8% 3.6%
Embodiment 4 1.3% 2.1% 3.1%
Embodiment 5 1.1% 2.5% 2.8%
Embodiment 6 1.3% 2.2% 3.9%
Embodiment 7 1.0% 3.1% 3.1%
Embodiment 8 1.4% 2.1% 3.3%
Embodiment 9 1.2% 2.8% 3.7%
This product is not used 15% 25% 30%
It is from table 1 it follows that substantially low using the incidence of disease of water body animals and plants after the gemma bacterium powder of the invention obtained The incidence of disease of water body animals and plants in the case of this product is not used.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, although with reference to foregoing reality Apply example the present invention is described in detail, for those skilled in the art, it still can be to foregoing each implementation Technical scheme described in example is modified, or carries out equivalent substitution to which part technical characteristic.All essences in the present invention God any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection with principle.

Claims (6)

1. a kind of gemma bacterium powder, it is characterised in that be made up of by mass percentage following components:Bacillus subtilis 31.1~ 70%th, bacillus licheniformis 20.8~50%, bacillus megaterium 4~16%, vitamin C 2~5%, high active enzyme 0.03~ 0.1%.
A kind of 2. gemma bacterium powder according to claim 1, it is characterised in that the high active enzyme be protease, amylase, One or more in cellulase, lipase, pectase, mannonase peroxidase.
3. a kind of preparation method of gemma bacterium powder, it is characterised in that this method is achieved by the steps of:
Step 1, following components is weighed respectively by mass percentage:Bacillus subtilis 31.1~70%, bacillus licheniformis 20.8~50%, bacillus megaterium 4~16%, vitamin C 2~5%, high active enzyme 0.03~0.1%;
Step 2, it is the bacillus subtilis described in step 1, bacillus licheniformis, bacillus megaterium, vitamin C, height is living Property enzyme add in mixer and stir, and stand, obtain the first mixed material;
Step 3, concentration is dried in the first mixed material obtained to the step 2, obtains gemma bacterium powder.
4. the preparation method of a kind of gemma bacterium powder according to claim 3, it is characterised in that described quiet in the step 2 The time put is 30-50min.
5. the preparation method of a kind of gemma bacterium powder according to claim 4, it is characterised in that described quiet in the step 2 The temperature put is 30~50 DEG C.
A kind of 6. preparation method of gemma bacterium powder according to claim 3-5 any one, it is characterised in that the step In 3, the time of the dry concentration is 20~50min.
CN201711225355.0A 2017-11-29 2017-11-29 A kind of gemma bacterium powder and preparation method thereof Pending CN107858307A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201711225355.0A CN107858307A (en) 2017-11-29 2017-11-29 A kind of gemma bacterium powder and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201711225355.0A CN107858307A (en) 2017-11-29 2017-11-29 A kind of gemma bacterium powder and preparation method thereof

Publications (1)

Publication Number Publication Date
CN107858307A true CN107858307A (en) 2018-03-30

Family

ID=61704385

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201711225355.0A Pending CN107858307A (en) 2017-11-29 2017-11-29 A kind of gemma bacterium powder and preparation method thereof

Country Status (1)

Country Link
CN (1) CN107858307A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111972443A (en) * 2020-07-21 2020-11-24 安徽美自然环境科技有限公司 Biological agent for inhibiting growth of filamentous green algae
CN112522163A (en) * 2020-12-25 2021-03-19 南京升鳌生物环保科技有限公司 Microbial repairing agent for eliminating old green water and preparation method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101109008A (en) * 2006-07-18 2008-01-23 上海四季生物科技有限公司 Aquifer amendment containing multiple active microorganisms and method of preparing the same
US20160037781A1 (en) * 2014-08-06 2016-02-11 Envera, Llc Bacterial spore compositions for industrial uses
CN106173270A (en) * 2016-07-16 2016-12-07 青岛东海药业有限公司 A kind of compound micro-ecological preparation and application thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101109008A (en) * 2006-07-18 2008-01-23 上海四季生物科技有限公司 Aquifer amendment containing multiple active microorganisms and method of preparing the same
US20160037781A1 (en) * 2014-08-06 2016-02-11 Envera, Llc Bacterial spore compositions for industrial uses
CN106173270A (en) * 2016-07-16 2016-12-07 青岛东海药业有限公司 A kind of compound micro-ecological preparation and application thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DE ALENCAR FLS等: "The use of bacterial biomediation of metals in aquatic environments in the twenty-first century:a systematic review.", 《ENVIRON SCI POLLUT RES INT.》 *
尹军霞等: "β-葡聚糖和乳酸杆菌对鲫协同作用的初步研究", 《大连水产学院学报》 *
李华文等: "饲用芽孢杆菌的生物学功能及其在水产养殖中的应用 ", 《饲料与畜牧》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111972443A (en) * 2020-07-21 2020-11-24 安徽美自然环境科技有限公司 Biological agent for inhibiting growth of filamentous green algae
CN112522163A (en) * 2020-12-25 2021-03-19 南京升鳌生物环保科技有限公司 Microbial repairing agent for eliminating old green water and preparation method and application thereof

Similar Documents

Publication Publication Date Title
CN106380004B (en) Aquaculture waters restoration of the ecosystem agent and preparation method thereof
CN106035171A (en) Prawn ecological culturing method based on microbial regulation
CN108147634A (en) Bottom material of pond for breeding improvement effervescent agent and its preparation method and application
CN105284675B (en) A kind of biological feed preparation method for breeding loach
CN106688999A (en) Method for breeding South America white shrimps
CN116439174B (en) Seedling cultivation method for Babylonia
CN106316021A (en) Slit conditioner for agricultural ponds and preparing method thereof
CN104710003B (en) A kind of scavenging agent improving shellfish culture environment
CN108813223A (en) A kind of aquaculture specific complex microorganism formulation and preparation method thereof
CN110451657A (en) A kind of aquaculture water quality improving agent
CN103392641A (en) Precision pond culture method for sepiella maindroni de Rochebrune
CN104186431A (en) High-density artemia breeding method with single-cell protein single-step food chain utilized
CN107858307A (en) A kind of gemma bacterium powder and preparation method thereof
CN109020667A (en) A kind of method and rich water material using black soldier flies cultivation excrement slag production rich water material
CN109952983A (en) A kind of cultural method of Penaeus Vannmei
CN107307002A (en) A kind of Bacillus antidote
CN112913967A (en) Shrimp, crab and fishpond manure water paste, bait for cultivating water spider organisms and preparation method
CN107969357A (en) A kind of high-efficient method for cultivating of fish fry
CN103651201B (en) A kind of method of preventing and treating prawn ' s virus evil raising prawn culturing output
CN106386588B (en) A kind of ecological cultivation method of Fugu rubripes
JP2000342248A (en) Thermophilic spawn, organic fertilizer and liquid organic fertilizer, and production thereof
CN108002900A (en) A kind of rich water agent for aquaculture and preparation method thereof
CN109292998A (en) A kind of EM bacterium improver of water quality of green agriculture
Dewi et al. Water quality in the maintenance of oreochromis niloticus through environmentally friendly biofloc technology
CN106747728A (en) A kind of viable bacteria fermentation fertilizer and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20180330