CN103932198A - Preparation method for selenium-enriched glutathione beer yeast biological product by utilizing waste beer yeast - Google Patents
Preparation method for selenium-enriched glutathione beer yeast biological product by utilizing waste beer yeast Download PDFInfo
- Publication number
- CN103932198A CN103932198A CN201410134408.8A CN201410134408A CN103932198A CN 103932198 A CN103932198 A CN 103932198A CN 201410134408 A CN201410134408 A CN 201410134408A CN 103932198 A CN103932198 A CN 103932198A
- Authority
- CN
- China
- Prior art keywords
- yeast
- glutathione
- selenium
- enriched
- beer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 103
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 title claims abstract description 103
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 title claims abstract description 87
- 239000011669 selenium Substances 0.000 title claims abstract description 76
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 63
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 63
- 229960003180 glutathione Drugs 0.000 title claims abstract description 43
- 108010024636 Glutathione Proteins 0.000 title claims abstract description 39
- 239000002699 waste material Substances 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 235000013405 beer Nutrition 0.000 claims abstract description 18
- 238000001035 drying Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 4
- 239000002243 precursor Substances 0.000 claims abstract description 3
- 229940091258 selenium supplement Drugs 0.000 claims description 52
- UOYQOJGTLUOLDS-GEMLJDPKSA-N (2s)-2-amino-5-[[(2r)-1-(carboxymethylamino)-1-oxo-3-sulfanylpropan-2-yl]amino]-5-oxopentanoic acid;selenium Chemical compound [Se].OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O UOYQOJGTLUOLDS-GEMLJDPKSA-N 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 14
- 235000013336 milk Nutrition 0.000 claims description 13
- 239000008267 milk Substances 0.000 claims description 13
- 210000004080 milk Anatomy 0.000 claims description 13
- 238000006243 chemical reaction Methods 0.000 claims description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 10
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 9
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 9
- 235000015921 sodium selenite Nutrition 0.000 claims description 9
- 210000005253 yeast cell Anatomy 0.000 claims description 8
- 239000004471 Glycine Substances 0.000 claims description 7
- 230000015572 biosynthetic process Effects 0.000 claims description 7
- 229960002989 glutamic acid Drugs 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 6
- 229960001471 sodium selenite Drugs 0.000 claims description 6
- 239000011781 sodium selenite Substances 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 5
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 5
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
- 239000000084 colloidal system Substances 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 238000005469 granulation Methods 0.000 claims description 5
- 230000003179 granulation Effects 0.000 claims description 5
- 235000019534 high fructose corn syrup Nutrition 0.000 claims description 5
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims description 5
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims description 5
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims description 5
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 210000001082 somatic cell Anatomy 0.000 claims description 5
- 230000036983 biotransformation Effects 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 239000000796 flavoring agent Substances 0.000 claims description 3
- 235000019634 flavors Nutrition 0.000 claims description 3
- 239000004310 lactic acid Substances 0.000 claims description 3
- 235000014655 lactic acid Nutrition 0.000 claims description 3
- 238000003786 synthesis reaction Methods 0.000 claims description 3
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 2
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 2
- 235000015165 citric acid Nutrition 0.000 claims description 2
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 claims description 2
- 239000001630 malic acid Substances 0.000 claims description 2
- 235000011090 malic acid Nutrition 0.000 claims description 2
- 208000035404 Autolysis Diseases 0.000 claims 2
- 206010057248 Cell death Diseases 0.000 claims 2
- 241001597008 Nomeidae Species 0.000 claims 2
- 239000000853 adhesive Substances 0.000 claims 2
- 230000001070 adhesive effect Effects 0.000 claims 2
- 150000007524 organic acids Chemical class 0.000 claims 2
- 230000028043 self proteolysis Effects 0.000 claims 2
- 230000008030 elimination Effects 0.000 claims 1
- 238000003379 elimination reaction Methods 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 235000013305 food Nutrition 0.000 claims 1
- 239000012535 impurity Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 239000000047 product Substances 0.000 abstract description 18
- 150000001413 amino acids Chemical class 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 5
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 230000003647 oxidation Effects 0.000 abstract description 2
- 238000007254 oxidation reaction Methods 0.000 abstract description 2
- 235000013343 vitamin Nutrition 0.000 abstract description 2
- 239000011782 vitamin Substances 0.000 abstract description 2
- 229940088594 vitamin Drugs 0.000 abstract description 2
- 229930003231 vitamin Natural products 0.000 abstract description 2
- 238000004140 cleaning Methods 0.000 abstract 1
- 230000007812 deficiency Effects 0.000 abstract 1
- 239000003623 enhancer Substances 0.000 abstract 1
- 230000036039 immunity Effects 0.000 abstract 1
- 239000002440 industrial waste Substances 0.000 abstract 1
- 238000003801 milling Methods 0.000 abstract 1
- 238000005453 pelletization Methods 0.000 abstract 1
- 238000004064 recycling Methods 0.000 abstract 1
- 239000013589 supplement Substances 0.000 abstract 1
- 230000003407 synthetizing effect Effects 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
- 235000003969 glutathione Nutrition 0.000 description 23
- 238000004519 manufacturing process Methods 0.000 description 11
- 238000000034 method Methods 0.000 description 11
- 102000008114 Selenoproteins Human genes 0.000 description 9
- 108010074686 Selenoproteins Proteins 0.000 description 9
- 235000001014 amino acid Nutrition 0.000 description 6
- 229940024606 amino acid Drugs 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 5
- 235000018417 cysteine Nutrition 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 235000013922 glutamic acid Nutrition 0.000 description 5
- 239000004220 glutamic acid Substances 0.000 description 5
- 229940081969 saccharomyces cerevisiae Drugs 0.000 description 5
- 239000008280 blood Substances 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 241000190950 Rhodopseudomonas palustris Species 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 238000005374 membrane filtration Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 150000002978 peroxides Chemical class 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- FDKWRPBBCBCIGA-REOHCLBHSA-N (2r)-2-azaniumyl-3-$l^{1}-selanylpropanoate Chemical compound [Se]C[C@H](N)C(O)=O FDKWRPBBCBCIGA-REOHCLBHSA-N 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 2
- 241000235646 Cyberlindnera jadinii Species 0.000 description 2
- FDKWRPBBCBCIGA-UWTATZPHSA-N D-Selenocysteine Natural products [Se]C[C@@H](N)C(O)=O FDKWRPBBCBCIGA-UWTATZPHSA-N 0.000 description 2
- 108010053070 Glutathione Disulfide Proteins 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 150000001945 cysteines Chemical class 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013373 food additive Nutrition 0.000 description 2
- 239000002778 food additive Substances 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- YPZRWBKMTBYPTK-BJDJZHNGSA-N glutathione disulfide Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@H](C(=O)NCC(O)=O)CSSC[C@@H](C(=O)NCC(O)=O)NC(=O)CC[C@H](N)C(O)=O YPZRWBKMTBYPTK-BJDJZHNGSA-N 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 230000007096 poisonous effect Effects 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- ZKZBPNGNEQAJSX-UHFFFAOYSA-N selenocysteine Natural products [SeH]CC(N)C(O)=O ZKZBPNGNEQAJSX-UHFFFAOYSA-N 0.000 description 2
- 235000016491 selenocysteine Nutrition 0.000 description 2
- 229940055619 selenocysteine Drugs 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- FDKWRPBBCBCIGA-UHFFFAOYSA-N 2-azaniumyl-3-$l^{1}-selanylpropanoate Chemical compound [Se]CC(N)C(O)=O FDKWRPBBCBCIGA-UHFFFAOYSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 102000006587 Glutathione peroxidase Human genes 0.000 description 1
- 108700016172 Glutathione peroxidases Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000235088 Saccharomyces sp. Species 0.000 description 1
- -1 Se-enriched yeast Chemical compound 0.000 description 1
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical class IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 238000011953 bioanalysis Methods 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 208000030303 breathing problems Diseases 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- UUYVRXVWXDDDGX-WDSKDSINSA-N glutathioselenol Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS[SeH])C(=O)NCC(O)=O UUYVRXVWXDDDGX-WDSKDSINSA-N 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 150000002440 hydroxy compounds Chemical class 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 229940082569 selenite Drugs 0.000 description 1
- MCAHWIHFGHIESP-UHFFFAOYSA-L selenite(2-) Chemical compound [O-][Se]([O-])=O MCAHWIHFGHIESP-UHFFFAOYSA-L 0.000 description 1
- GJEZZQVPWMCGSB-BJDJZHNGSA-N selenodiglutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@H](C(=O)NCC(O)=O)CS[Se]SC[C@@H](C(=O)NCC(O)=O)NC(=O)CC[C@H](N)C(O)=O GJEZZQVPWMCGSB-BJDJZHNGSA-N 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000005495 thyroid hormone Substances 0.000 description 1
- 229940036555 thyroid hormone Drugs 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/14—Yeasts or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/20—Agglomerating; Granulating; Tabletting
- A23P10/28—Tabletting; Making food bars by compression of a dry powdered mixture
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Inorganic Chemistry (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A selenium-enriched glutathione beer yeast biological product is characterized in that waste beer yeast is used for transforming inorganic selenium into organic selenium, and glutathione precursor amino acids are used for synthetizing glutathione, and thus the selenium-enriched glutathione yeast is obtained. The beer yeast biological product provided by the invention not only realizes the recycling of industrial waste of beer, but the glutathione also can promote the activity of the yeast selenium, which makes up deficiency of single selenium enriching in the existing products. The selenium-enriched glutathione beer yeast biological product not only has the effects of oxidation resistance, enhancement of human immunity and so on; at the same time, the selenium-enriched glutathione beer yeast biological product is a high efficient nutrient enhancer and supplement by providing a plurality of amino acids and vitamins to the human body. A preparation method for the selenium-enriched glutathione beer yeast biological product is characterized in that the yeast liquid biotransformed by the organic selenium and the glutathione is subjected to centrifuging, cleaning, heavy suspending, high-pressure homogenizing, colloidal milling, cold drying, pelletizing and tablet compressing to obtain the swallowable or chewable selenium-enriched glutathione yeast tablets. The selenium-enriched glutathione yeast tablets have the advantages of being convenient to eat, stable in components and easy to preserve.
Description
Technical field
The present invention relates to utilize fresh beer waste yeast to carry out the synthetic Organic Selenium of bio-transformation of sodium selenite, transform glutathione precursor amino acid synthesizing glutathion simultaneously, prepare the method for rich selenium glutathione yeast bio goods.
Background technology
Selenium is one of essential trace element of the mankind and livestock and poultry, the biological function of selenium be it is generally acknowledged and mainly come from selenoprotein (selenoprotein), the biosynthesis of selenoprotein is by the codon UGA coding selenocystein (selenocysteine on mRNA, Sec) by selenium to be covalently bound in protein, thereby the biosynthesis of selenoprotein and metabolic mechanism have important theory value for the subject such as molecular biology and biochemistry.Quantity research shows greatly, and selenoprotein has important function in antioxidation activity, adjusting redox signal, adjusting thyroid hormone metabolism and immune response.
Research shows, human body safety using amount minimum and optimum picked-up selenium every day is respectively 22 μ g and 50 μ g, and Safe maximum Se intake is 400 ~ 550 μ g.Data show, a lot of national residents' in the whole world selenium intake is lower than the suitableeest selenium intake, and China resident's selenium intake of enumeration district (ED) and blood Se content all lower than the U.S. and Japan.In addition, there are some researches show, some special ill crowds also need to mend selenium, as the Diseases such as diabetes, angiocardiopathy, disease of digestive tract, breathing problem and cancer patient's blood Se content is starkly lower than healthy population.Therefore, no matter Selenium Supplement element, be as feed addictive or as meals or medicine replenishers, all has great importance and wide prospect.
Compared with inorganic selenium, Organic Selenium has the features such as high biological absorption, high usage and hypotoxicity, environmental pollution be little, thereby becomes the main Types of Selenium Supplement element.Organic selenium product adopts bioanalysis to transform inorganic selenium more at present, comprises animal conversion, Plant Transformation and microbial conversion.Wherein microorganism Organic Selenium, particularly Se-enriched yeast, as comparatively desirable Organic Selenium preparation, is paid attention to widely and is applied.
Rich selenium product has been realized suitability for industrialized production and has entered the practical stage in many countries.Japan, the U.S. are in the existing yeast Organic Selenium merchandise sales 1980s, and all there is Se-enriched yeast in the European countries such as France, Finland and Germany as food additives and drug vending.The rich selenium product of China is in research and development and production phase widely.As Anhui Tiger Biotechnology Co., Ltd. provide a kind of with Yeast strain of beer (
saccharomyces sp., ATCC 21135) and fermented and cultured, the method for producing Se-enriched yeast, Se content can reach 2000 μ g/g above (CN 102277306 B); Guangzhou Glam Biotechnology Co., Ltd. with Rhodopseudomonas palustris (
rhodopseudomonas palustris) be rich selenium material, the preparation method (CN 103284029 A) of rich selenium Rhodopseudomonas palustris preparation is provided, the conversion ratio of selenium reaches 90.2%, and the rich selenium amount of thalline reaches 75.3 mg/g dry mycelium.
One of subject matter that rich selenium product exists is expensive.In the biosynthesis of Enrichment by Microorganisms selenium research at present, almost all adopt pure-blood ferment method.Pure-blood ferment needs fairly large early investment, and incubation time is longer, lot of energy, and operating condition requires strict, makes production cost higher, and therefore Se-enriched bio goods price is generally higher.
Glutathione is divided into reduced glutathione (GSH) and oxidized form of glutathione (GSSG), and usually said glutathione is reduced glutathione.Glutathione is the important active substances in body, and it has the free radical of removing, detoxifies and maintain the biosynthesis of DNA and the different physiological roles such as normal growth and cellular immunity of cell, has important using value in medical science and healthcare field.Glutathione is also natural components intrinsic in brewer's yeast, can effectively improve the content of beer yeast cells GSH-PX activity by add glutamic acid, cysteine and glycine in culture propagation culture medium.Glutathione peroxidase (GSH-Px) is a kind of important peroxide breakdown enzyme extensively existing in body.Selenium is the constituent of GSH-Px enzyme system, and it can become GSSG by catalysis GSH, makes poisonous peroxide be reduced into nontoxic hydroxy compounds, promotes H simultaneously
2o
2decomposition, thereby the structure of Cell protection film and function are not subject to interference and the infringement of peroxide, therefore glutathione is the important medium that selenium element is brought into play physiologically active in human body.Selenium and glutathione (GSH) system plays key effect in oxidation defense reaction, prepare rich selenium glutathione yeast, the health care of glutathione not only can be provided, can better promote the physiological function of selenium element, thereby bring into play more fully biologically active simultaneously.For example, Biological Selenium and glutathione are made an addition in fish feed simultaneously, can greatly strengthen fish opposing and the decomposition discharge capacity (CN 101438768A) of poisonous substance to external world.University Of Suzhou, taking candida utili as starting strain, provides a kind of method (Chinese CN 101875958 A) that improves glutathione content in selenium-enriched Candida utilis.
Beer waste yeast is main accessory substance during brewing industry is produced.It is estimated that 100 tons of beer of every production approximately can obtain 1.5 ~ 2.0 tons of waste beer yeasts (moisture 75% ~ 80%).Along with China's brewing industry fast development, beer annual production is with 5% ~ 7% speed increase, the data of announcing according to State Statistics Bureau, China's beer production in 2012 is 4,902 ten thousand kilolitres, and waste yeast accounts for 1.5% of beer production, wherein only have 40%-50% waste yeast to be recovered utilization, all the other go out of use, serious environment pollution (BOD of yeast paste and COD are all up to more than 100000 mg/L).At present, the comprehensive utilization great majority of beer waste yeast are concentrated on to protein, nucleic acid, vitamin, polysaccharide and the enzyme aspect to wherein, be mainly the preparation research of flavor food additive yeast extract, and still belong to blank for Sync enrichment conversion inorganic selenium and the biosynthetic Application and Development research of glutathione.
The bacterial classification overwhelming majority that production Se-enriched yeast adopts is saccharomyces cerevisiae, identical with Beer Brewage bacterial classification used, thereby pass through taking beer waste yeast as raw material to ferment conversion inorganic selenium and synthesizing glutathion again, feasible in theory; Simultaneously, have and studies show that the main period of the synthetic Organic Selenium of saccharomyces cerevisiae and glutathione is the stationary phase of Yeast Cultivation after 48 hours, and fresh waste yeast cell is mostly also in anaerobic fermentation period of 48 ~ 72 hours, therefore fresh waste yeast has the ability of enrichment Organic Selenium and glutathione synthesis on opportunity, possesses the feasibility turning waste into wealth.
Yeast product need carry out certain broken wall or self-dissolving processing conventionally, is beneficial to digesting and assimilating of animal body.Rich selenium glutathione yeast prepared by this method 2 hours self-dissolving rates under simulation gastric environment, only in 16% left and right, can, by adding appropriate sodium chloride as self-dissolving catalyst, promote the self-dissolving process of yeast, are beneficial to digestion and the absorption of animal body.
Summary of the invention
For the high problem of the existing purebred microorganism fermentation rich selenium production cost of preparation, the object of this invention is to provide a kind of method of utilizing beer waste yeast to prepare rich selenium glutathione yeast.
Another object of the present invention is to provide a kind of rich selenium glutathione yeast product and preparation method thereof.
The technical solution adopted in the present invention is:
Beer waste yeast (saccharomyces cerevisiae) (
saccharomycescerevisiae), from the fresh undressed beer mud of Tsingtao beer (Jinan) Co., Ltd.
With culture medium by resuspended above-mentioned used yeast slurry, and add sodium selenite and glutathione synthesis precusor amino acids (cysteine, glutamic acid and glycine), shaking flask or fermentation tank culture transform inorganic selenium in 16 ~ 20 hours and precusor amino acids is prepared rich selenium glutathione yeast, wherein in every gram of dry rich selenium glutathione yeast, contain Organic Selenium 1000 ~ 1500 μ g, account for 82 ~ 92% of total selenium weight, selenoprotein content accounts for 75 ~ 80% of total selenium weight; In every gram of dry rich selenium glutathione yeast, contain glutathione approximately 6 mg simultaneously.
This method adopts adds 3 ~ 6 g/L NaCl, and under simulation gastric environment, the self-dissolving rate of 2 hours Se-enriched yeasts can reach 36%, can effectively improve the bioavailability of yeast product.
A method of producing selenium-rich Saccharomyces cerevisiae, comprises the steps:
1) fresh waste beer yeast is after washing, and centrifugal 5 ~ 10 minutes of 4000 r/min, obtain yeast paste (moisture content is in 58% left and right), add the culture medium that contains sodium selenite and precusor amino acids, ° C fermentation 12 ~ 16 hr in 24 ° of C ~ 28;
2) described medium component is: phosphoric acid 8.0 ~ 10.0 g/L, sodium selenite 18 ~ 20 mg/L, ammonium sulfate 8.0 ~ 10.0 g/L, magnesium sulfate 3.0 ~ 5.0 g/L, calcium chloride 0.1 ~ 0.4 g/L, glucose 60.0 ~ 80.0 g/ L, pH5.0,1 ~ 2 g/L glutamic acid, 0.5 ~ 1.5 g/L glycine, 0.8 ~ 1.5 g/L cysteine (amino acid needs independent sterilizing), every 1L nutrient solution adds 150 ~ 250 g yeast pastes (weight in wet base);
3) gained yeast juice is after centrifugal, obtain somatic cells, after deionized water washing 3 times, the resuspended yeast cells of lactic acid solution with 2 ~ 5%, obtain yeast milk, this yeast milk is through high-pressure homogeneous homogeneous repeatedly 2 ~ 4 times, then adds 1 ~ 2% Hydroxypropyl methylcellulose and 10% ~ 20% 65% HFCS, then cross colloid mill, obtain the system of homogeneous.This system, through low temperature drying 30 ~ 60 hours, makes its water content reduce to 1 ~ 5%, obtains rich selenium glutathione yeast product through granulation and compressing tablet.
In the present invention, also the rich selenium glutathione yeast obtaining can be promoted to the self-dissolving of yeast cells by adding 3 ~ 6 g/L sodium chloride, thereby increase it the digesting and assimilating of stomach, be conducive to its biological utilisation in human body.
Inorganic selenium is in the conversion process of Organic Selenium in vivo, and reduced glutathione (GSH) plays an important role.GSH, as the reducing agent of selenite, completes the building-up process from sodium selenite to selenocysteine by generating GSSeSG and GSSeH.In the present invention, by adding the precusor amino acids of glutathione both to improve the synthetic of the conversion of beer waste yeast to inorganic selenium and Organic Selenium, can obtain again the yeast glutathione of high yield.
Detailed description of the invention
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
5.0 g phosphoric acid, 4.0 g ammonium sulfate, 1.8g magnesium sulfate, 0.15 g calcium chloride, 9.0 mg sodium selenites and 30 g glucose are dissolved in 400 mL running water, adjust pH to 5. 0,115 ° of C, high pressure moist heat sterilization 30 min in 2000 mL triangular flasks; 0.1 g glutamic acid, 0.05 g glycine, 0.075 g cysteine are dissolved in 100 mL distilled water, with after 0.22 μ m membrane filtration degerming, integrate with in the 2000 mL triangular flasks that fill sterile medium.Add and clean centrifugal fresh yeast mud 100 g, 28 ± 1 ° of C, 150 rpm cultivate 18 h.
Result shows, measures Se content 1101 μ g/g yeast in yeast by conventional method, and wherein organic selenium content accounts for 90%, and selenoprotein content accounts for 78.6%, contains glutathione approximately 6.8 mg in every gram of rich selenium glutathione dry ferment.
Gained yeast juice is after centrifugal, obtain somatic cells, after deionized water washing 3 times, the resuspended yeast cells of lactic acid solution with 3%, obtains yeast milk, to the NaCl that adds 6 g/L in yeast milk, and stir, this yeast milk is through high-pressure homogeneous 2 times (80 MPa), then adds 1.5% Hydroxypropyl methylcellulose and 12% 65% HFCS, then cross colloid mill 2 times, obtain the system of homogeneous.This system, through low temperature drying 45 hours, makes its water content reduce to 2%, and gained solid content obtains rich selenium glutathione yeast product through granulation and compressing tablet.
Embodiment 2
50.0 g phosphoric acid, 13.4 g ammonium sulfate, 20 g magnesium sulfate, 2 g calcium chloride, 33.4 mg sodium selenites and 300.0 g glucose are dissolved in 4900 mL running water, adjust pH to 5. 0,115 ° of C, high pressure moist heat sterilization 20 min in 10 L fermentation tanks; 10 g glutamic acid, 7.5 g cysteines, 5 g glycine are dissolved in 100 mL distilled water, with after 0.22 μ m membrane filtration degerming, integrate with and fill in sterilized nutrient solution.Add and clean centrifugal fresh yeast mud 1250 g, 28 ± 1 ° of C of temperature, mixing speed 200 ~ 500 rpm, keep dissolved oxygen to be greater than 25%, cultivate and transform 19 hours.
Result shows, measures Se content 1223 μ g/g yeast in yeast by conventional method, and wherein organic selenium content accounts for 91.0%, and selenoprotein content accounts for 77.8%, and glutathione content is 8.03 mg/g dry ferments.
Gained yeast conversion liquid is after centrifugal, obtain somatic cells, after deionized water washing 2 times, the resuspended yeast cells of citric acid solution with 5%, obtains yeast milk, in yeast milk, add 5 g NaCl, and stir, this yeast milk is through high-pressure homogeneous 3 times (75 MPa), then adds 1.2% Hydroxypropyl methylcellulose and 15% 65% HFCS, then cross colloid mill 3 times, obtain the system of homogeneous.This system, through low temperature drying 55 hours, makes its water content reduce to 1.5%, and gained solid content obtains rich selenium glutathione yeast product through granulation and compressing tablet.
Embodiment 3
150.0 g phosphoric acid, 40 g ammonium sulfate, 50 g magnesium sulfate, 5.5 g calcium chloride, 100 mg sodium selenites, 900.0 g glucose are dissolved in 14 L running water, adjust pH to 5. 0,115 ° of C, high pressure moist heat sterilization 25 min in 30 L fermentation tanks; 35 g glutamic acid, 25 g cysteines, 15 g glycine are dissolved in 1L distilled water, with after 0.22 μ m membrane filtration degerming, integrate with and fill in sterilized nutrient solution.Add and clean centrifugal fresh yeast mud 4000 g, 29 ± 1 ° of C of temperature, mixing speed 200 ~ 500 rpm, keep dissolved oxygen to be greater than 30%, cultivate and transform 17 hours.
Result shows, measures Se content 1424 μ g/g yeast in yeast by conventional method, and wherein organic selenium content accounts for 90%, and selenoprotein content accounts for 80.1%, and glutathione content is 7.93 mg/g dry ferments.
Gained yeast conversion liquid is after centrifugal, obtain somatic cells, after deionized water washing 3 times, the resuspended yeast cells of malic acid solution with 4%, obtains yeast milk, to the NaCl that adds 5 g/L in yeast milk, and stir, this yeast milk is through high-pressure homogeneous 2 times (90 MPa), then adds 1% Hydroxypropyl methylcellulose and 18% 65% HFCS, then cross colloid mill 2 times, obtain the system of homogeneous.This system, through low temperature drying 52 hours, makes its water content reduce to 1.2%, and gained solid content obtains rich selenium glutathione yeast product through granulation and compressing tablet.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (4)
1. rich selenium glutathione brewer's yeast biological products, by beer waste yeast bio-transformation inorganic selenium and the amino acquisition of glutathione precursor.
2. described in claim 1, saccharomyces cerevisiae is the useless fresh yeast mud of brewing industry, and the step of its Synthesis Organic Selenium and glutathione is as follows:
(1) running water washing 3 times for fresh waste beer yeast, through 4000 r/min centrifugal 5 ~ 10 minutes, obtains the yeast paste of debitterize, impurity elimination;
(2) preparation Organic Selenium and glutathione conversion fluid, its composition is: phosphoric acid 8.0 ~ 10.0 g/L, sodium selenite 18 ~ 20 mg/L, ammonium sulfate 8.0 ~ 10.0 g/L, magnesium sulfate 3.0 ~ 5.0 g/L, calcium chloride 0.1 ~ 0.4 g/L, glucose 60.0 ~ 80.0 g/ L, pH5.0,1 ~ 2 g/L glutamic acid, 0.5 ~ 1.5 g/L glycine, 0.8 ~ 1.5g/L cysteine;
(3) to adding 150 ~ 250 g yeast paste that wets in every liter of yeast conversion liquid in (2), and be uniformly dispersed;
(4) by the system in (3) in constant-temperature table or bioreactor under 28 ± 1 ° of C, 150 ~ 500 rpm conditions, cultivate and bio-transformation 18 ~ 22 hours.
3. rich selenium glutathione saccharomyces cerevisiae biological products according to claim 1, its preparation process is:
(1) after cultivation and bio-transformation finish, medium centrifugal obtains somatic cells, through deionized water washing, with 2 ~ 5% the resuspended yeast cells of food grade organic acid's solution, and adds yeast autolysis derivant, obtains the yeast milk of acidifying;
(2) yeast milk of acidifying is through high-pressure homogeneous 2 ~ 4 times, then adds 1 ~ 2% adhesive and 10% ~ 20% flavor enhancement, and stirs;
(3) system in (2) is crossed to colloid mill 2 ~ 3 times, obtain the yeast product system of homogeneous;
(4) by the system in (3) through low temperature drying 40 ~ 60 hours, become water content and be 1 ~ 3% solid content;
(5) gained solid content in (4) is obtained to rich selenium glutathione yeast product through granulation and compressing tablet.
4. according to claim 3 in rich selenium glutathione saccharomyces cerevisiae biological products preparation process, yeast autolysis derivant used is 3 ~ 6 g/L NaCl; Adhesive therefor is 1 ~ 2% Hydroxypropyl methylcellulose; Flavor enhancement used is 10 ~ 20% 65% HFCS; Selected organic acid is lactic acid, citric acid, malic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410134408.8A CN103932198A (en) | 2014-04-04 | 2014-04-04 | Preparation method for selenium-enriched glutathione beer yeast biological product by utilizing waste beer yeast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410134408.8A CN103932198A (en) | 2014-04-04 | 2014-04-04 | Preparation method for selenium-enriched glutathione beer yeast biological product by utilizing waste beer yeast |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103932198A true CN103932198A (en) | 2014-07-23 |
Family
ID=51180284
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410134408.8A Pending CN103932198A (en) | 2014-04-04 | 2014-04-04 | Preparation method for selenium-enriched glutathione beer yeast biological product by utilizing waste beer yeast |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103932198A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104757560A (en) * | 2014-08-11 | 2015-07-08 | 四川安益生物科技有限公司 | Glutathione yeast enzymolysis solution composition and preparation method thereof |
| CN105624003A (en) * | 2015-11-27 | 2016-06-01 | 济南大学 | Anti-oxidation and anti-aging fruit vinegar and preparation method therefor |
| CN105663021A (en) * | 2016-02-19 | 2016-06-15 | 四川安益生物科技有限公司 | Glutathione fermented mask and preparation method thereof |
| CN107586719A (en) * | 2017-10-30 | 2018-01-16 | 武汉轻工大学 | It is a kind of to have both anti-oxidant and selenium-supply function yeast product and preparation method thereof |
| CN109694829A (en) * | 2018-12-29 | 2019-04-30 | 嘉必优生物技术（武汉）股份有限公司 | A kind of selenium-rich saccharomyces cerevisiae, selenium-rich richness lycopene saccharomyces cerevisiae and preparation method thereof |
| KR20190131968A (en) * | 2018-05-18 | 2019-11-27 | 강원대학교산학협력단 | Selenium nanocolloid dispersion produced by hot melt extrusion method and uses thereof |
| CN112704225A (en) * | 2021-01-06 | 2021-04-27 | 青岛迪玛希国际电子商务有限公司 | Food leaven containing beer waste yeast extract and preparation method thereof |
| CN114540265A (en) * | 2022-03-23 | 2022-05-27 | 广州博善生物科技股份有限公司 | Preparation method of selenium-enriched yeast rich in glutathione |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0106146A1 (en) * | 1982-10-13 | 1984-04-25 | Hüls Aktiengesellschaft | Process for the biological preparation of glutathione |
| CN1606932A (en) * | 2003-10-14 | 2005-04-20 | 兰敬墨 | Preparation of health nutritious beverage for fat reduction using yeast |
| CN101255454A (en) * | 2008-03-14 | 2008-09-03 | 华东理工大学 | Method for biosynthesis of glutathione by using yeast |
| CN101875958A (en) * | 2009-11-10 | 2010-11-03 | 苏州大学 | Method for improving glutathione content in selenium-enriched Candida utilis |
| CN102860408A (en) * | 2012-09-24 | 2013-01-09 | 江西昌丰由由生物科技有限公司 | Preparation method of multifunctional live microorganism preparation for forage |
-
2014
- 2014-04-04 CN CN201410134408.8A patent/CN103932198A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0106146A1 (en) * | 1982-10-13 | 1984-04-25 | Hüls Aktiengesellschaft | Process for the biological preparation of glutathione |
| CN1606932A (en) * | 2003-10-14 | 2005-04-20 | 兰敬墨 | Preparation of health nutritious beverage for fat reduction using yeast |
| CN101255454A (en) * | 2008-03-14 | 2008-09-03 | 华东理工大学 | Method for biosynthesis of glutathione by using yeast |
| CN101875958A (en) * | 2009-11-10 | 2010-11-03 | 苏州大学 | Method for improving glutathione content in selenium-enriched Candida utilis |
| CN102860408A (en) * | 2012-09-24 | 2013-01-09 | 江西昌丰由由生物科技有限公司 | Preparation method of multifunctional live microorganism preparation for forage |
Non-Patent Citations (1)
| Title |
|---|
| 王正刚，等: "酵母天然酶系生物合成谷肤甘肤", 《生物技术》 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104757560A (en) * | 2014-08-11 | 2015-07-08 | 四川安益生物科技有限公司 | Glutathione yeast enzymolysis solution composition and preparation method thereof |
| CN105624003A (en) * | 2015-11-27 | 2016-06-01 | 济南大学 | Anti-oxidation and anti-aging fruit vinegar and preparation method therefor |
| CN105624003B (en) * | 2015-11-27 | 2018-06-12 | 济南大学 | A kind of fruit vinegar of resisting oxidation and delaying senility and preparation method thereof |
| CN105663021A (en) * | 2016-02-19 | 2016-06-15 | 四川安益生物科技有限公司 | Glutathione fermented mask and preparation method thereof |
| CN105663021B (en) * | 2016-02-19 | 2018-06-26 | 四川安益生物科技有限公司 | A kind of glutathione fermentation facial mask and preparation method thereof |
| CN107586719B (en) * | 2017-10-30 | 2020-03-17 | 武汉轻工大学 | Yeast product with functions of resisting oxidation and supplementing selenium and preparation method thereof |
| CN107586719A (en) * | 2017-10-30 | 2018-01-16 | 武汉轻工大学 | It is a kind of to have both anti-oxidant and selenium-supply function yeast product and preparation method thereof |
| KR102105827B1 (en) | 2018-05-18 | 2020-04-29 | 강원대학교산학협력단 | Selenium nanocolloid dispersion produced by hot melt extrusion method and uses thereof |
| KR20190131968A (en) * | 2018-05-18 | 2019-11-27 | 강원대학교산학협력단 | Selenium nanocolloid dispersion produced by hot melt extrusion method and uses thereof |
| CN109694829A (en) * | 2018-12-29 | 2019-04-30 | 嘉必优生物技术（武汉）股份有限公司 | A kind of selenium-rich saccharomyces cerevisiae, selenium-rich richness lycopene saccharomyces cerevisiae and preparation method thereof |
| CN109694829B (en) * | 2018-12-29 | 2022-09-13 | 嘉必优生物技术（武汉）股份有限公司 | Selenium-rich saccharomyces cerevisiae, selenium-rich lycopene-rich saccharomyces cerevisiae and preparation method thereof |
| CN112704225A (en) * | 2021-01-06 | 2021-04-27 | 青岛迪玛希国际电子商务有限公司 | Food leaven containing beer waste yeast extract and preparation method thereof |
| CN114540265A (en) * | 2022-03-23 | 2022-05-27 | 广州博善生物科技股份有限公司 | Preparation method of selenium-enriched yeast rich in glutathione |
| CN114540265B (en) * | 2022-03-23 | 2024-04-16 | 广州博善生物科技股份有限公司 | Preparation method of glutathione-enriched selenium-enriched yeast |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103932198A (en) | Preparation method for selenium-enriched glutathione beer yeast biological product by utilizing waste beer yeast | |
| CN103173371B (en) | Production of saccharomyces cerevisiae and lactobacillus acidophilus composite microbe preparation used for feed | |
| CN102094054B (en) | Production method of Bacillus subtilis antimicrobial lipopeptide and application of Bacillus subtilis antimicrobial lipopeptide in piglet feeds | |
| CN101543285B (en) | Wheat-bran dietary fiber composite functional fungus powder product and preparation process thereof | |
| CN103098986B (en) | Beneficial microorganism antibiont-free feed and preparation method thereof | |
| CN101914460B (en) | Method for fermenting phodotorula benthica culture | |
| CN103184174B (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN102443549A (en) | Acetic acid bacterium fermentation nutritive salt and use method thereof | |
| CN101508961A (en) | Method of preparing yeast rich in copper | |
| CN118592522A (en) | Feed rich in probiotics and active peptides and application thereof | |
| CN114107073A (en) | Method for producing hypha protein by utilizing molasses | |
| CN107853452A (en) | A kind of production method of additive for microbe feedstuff | |
| CN109123076A (en) | A kind of production method of livestock and poultry vitamin B2 auxotype probiotics | |
| CN101690541B (en) | Method for preparing feed protein from microbial fermented silkworms | |
| CN113416673A (en) | Complex microbial inoculant for detoxifying cottonseed meal as well as preparation method and application thereof | |
| CN116058432B (en) | Citric acid mycelium residue microbial feed additive and preparation method and application thereof | |
| CN107439793A (en) | The production method of feeding biologic ferment calcium | |
| CN109628350B (en) | Lactobacillus plantarum and application thereof | |
| CN107319173A (en) | A kind of utilization marc mycoprotein promotes the feed of young goose growth | |
| CN111802515A (en) | Biological calcium supplement and preparation and application method thereof | |
| CN101283730B (en) | Technological process for producing the single-cell protein by liquid state fermentation of potato marc | |
| CN109055266A (en) | A kind of biological agent and the preparation method and application thereof with vibrios growth in the inhibition shrimp pool | |
| CN114947017A (en) | Complete liquid fermented feed for fattening pigs and preparation method thereof | |
| CN1180700C (en) | Multifunctional fermented milk and its preparing method | |
| CN111213792A (en) | Biological fermentation feed for improving piglet anemia and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140723 |
|
| WD01 | Invention patent application deemed withdrawn after publication |