CN110004103A - A method of regulation nutritious spirulina elemental iron element accumulation - Google Patents

A method of regulation nutritious spirulina elemental iron element accumulation Download PDF

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CN110004103A
CN110004103A CN201910261367.1A CN201910261367A CN110004103A CN 110004103 A CN110004103 A CN 110004103A CN 201910261367 A CN201910261367 A CN 201910261367A CN 110004103 A CN110004103 A CN 110004103A
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李博生
徐青艳
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Beijing Forestry University
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Abstract

A kind of method that the present invention provides regulation nutritious spirulina elemental iron element accumulation is cultivated 3 days under conditions of 20-40 DEG C, 4-12klx, pH8.5-10.5 comprising steps of 1) spirulina is inoculated into culture medium；2) spirulina in step 1) is continued to cultivate under conditions of 2-20klx, pH8.5-11.0 at 15-35 DEG C, by the external source addition ferro element into culture medium, cultivates 3-7 days, obtain the spirulina of high nutrition element.The method of the present invention is substantially increased the content of ferro element in spirulina, makes nutrient in conjunction with intracellular organic matter, reduces to gastrointestinal irritation, improves absorption of human body utilization rate.Using the spirulina cells of this method culture iron content than traditional cultural method 175%；The organic degree of iron is followed successively by 89.98%.

Description

A method of regulation nutritious spirulina elemental iron element accumulation
The present invention is application number CN201611267287.X, a kind of entitled " regulation nutritious spirulina element accumulation Method " Chinese patent application divisional application.
Technical field
The present invention relates to biological cell post-directed training and extractive technique fields, specifically, being related to a kind of regulation spirulina The method of nutrient ferro element accumulation.
Background technique
In recent years, the supplement of nutrient has been to be concerned by more and more people.According to 1992 national nutrition survey number According to display, the calcium intake of compatriots only has 20%-50%, and the zinc-deficiency rate of children is up to 60%, and Chinese hypoferric anemia rate is average It is 20% or so, about 2/3 regional selenium deficiency.The conventional method of these nutrient deficiencies is solved, severe one passes through drug therapy, Less serious case is by meal supplement, and food supplement is generally unattainable needed by human body, and drug is mostly inorganic constituents supplement, such as chlorination Calcium, zinc sulfate etc., often absorption rate is low for human body.For this status, spirulina cells Nutrient Absorption and richness are utilized The characteristic of collection promotes its Nutrient Accumulation, inorganic ions organises in the cell, improve human body as carrier Absorptivity and utilization rate are reached for the purpose of human body extra-nutrition element.
Currently, the method for improving algae nutrient mainly reaches member by increasing external source culture solution intermediate ion concentration The purpose of element accumulation.However, domestic and international all multiple enterprises are related to region, season in its breeding process in cultivation exploitation spirulina Influence of the variation of section, temperature difference per day etc. to algal grown and nutritious substances accumulation is very big, but has no growing environment to spirulina The report of Nutrient Accumulation.Inventor by studying for many years, the results showed that environmental factor (temperature, environment, pH etc.) not only shadow The growth of spirulina is rung, and influences its physiological metabolism, the functions such as substance synthesis also have the accumulation of its mineral matter nutritional deep Influence.Effective Regulation nutrient organises for nutrient in spirulina cells accumulation, the supplement of human nutrition element, Spirulina application range is widened to be of great significance.
Summary of the invention
The object of the present invention is to provide a kind of raising nutritious spirulina elements (calcium, iron, zinc, selenium), and especially ferro element contains The method of amount.
In order to achieve the object of the present invention, the present invention regulates and controls the method for nutritious spirulina element accumulation, comprising the following steps:
1) spirulina is inoculated into culture medium, Yu Wendu 20-40 DEG C, illumination 4-12klx, the condition of pH value 8.5-10.5 Lower culture 3 days；
2) spirulina in step 1) is continued at 15-35 DEG C of temperature, illumination 2-20klx, the condition of pH value 8.5-11.0 Lower culture cultivates 3-7 days respectively by the external source addition calcium, iron, zinc, selenium element into culture medium, obtains the spiral shell of high nutrition element Revolve algae.
Method above-mentioned, step 2) are specific as follows: the spirulina in step 1) being continued to cultivate, adjusts temperature to 15-25 DEG C, light control adds external source CaCO into culture medium in 8.5-9.0 in 11-20klx, pH control3To final concentration 0.4- 0.8g/L, incubation time are 3-7 days, obtain the spirulina for being rich in calcium.Preferably, the spirulina in step 1) is continued to cultivate, Temperature is adjusted to 20 DEG C, and light control is controlled in 12klx, pH 8.5, and external source CaCO is added into culture medium3To final concentration 0.6g/L is cultivated 7 days, obtains the spirulina for being rich in calcium.
Method above-mentioned, step 2) are specific as follows: the spirulina in step 1) being continued to cultivate, temperature is adjusted to 25-35 DEG C, light control adds external source FeSO into culture medium in 10-11.0 in 2-6klx, pH control4·7H2O to final concentration 30- 60mg/L is cultivated 3-7 days, obtains rich iron-containing spirulina.Preferably, the spirulina in step 1) is continued to cultivate, temperature tune It saves to 30 DEG C, light control is controlled in 4klx, pH 10.5, and external source iron FeSO47H is added into culture medium2O is to final concentration 40mg/L is cultivated 3 days, obtains rich iron-containing spirulina.
Method above-mentioned, step 2) are specific as follows: the spirulina in step 1) being continued to cultivate, temperature is adjusted to 25-35 DEG C, light control adds external source ZnSO into culture medium in 8.5-9.5 in 6-11klx, pH control4·7H2O to final concentration 30- 70mg/L, incubation time are 3-7 days, obtain the spirulina for being rich in zinc.Preferably, the spirulina in step 1) is continued to cultivate, Temperature is adjusted to 30 DEG C, and light control is controlled in 8klx, pH 8.5, and external source zinc ZnSO is added into culture medium4·7H2O is to end Concentration 40mg/L is cultivated 3 days, obtains the spirulina for being rich in zinc.
Method above-mentioned, step 2) are specific as follows: the spirulina in step 1) being continued to cultivate, temperature is adjusted to 28-35 DEG C, light control adds external source selenium Na into culture medium in 9.0-10.0 in 11klx or more, pH control2SeO3To final concentration 80- 120mg/L, incubation time are 3-7 days, obtain the spirulina for being rich in selenium.Preferably, the spirulina in step 1) is continued to cultivate, Temperature is adjusted to 30 DEG C, and light control is controlled in 12klx, pH 9.5, and external source selenium Na is added into culture medium2SeO3To final concentration 100mg/L is cultivated 5 days, obtains the spirulina for being rich in selenium.
Preferably, step 1) is specific as follows: spirulina is inoculated into culture medium, at 30 DEG C, 12klx, and the item of pH value 9.5 It is cultivated 3 days under part.
In the specific embodiment of the present invention, the method for regulating and controlling nutritious spirulina element accumulation is specific as follows:
1) spirulina is inoculated into culture medium, is cultivated 3 days under conditions of 30 DEG C, 12klx, pH value 9.5；
2) spirulina in step 1) is continued to cultivate, temperature is adjusted to 20 DEG C, and light control exists in 12klx, pH control 8.5, external source CaCO is added into culture medium3It to final concentration 0.6g/L, cultivates 7 days, obtains the spirulina for being rich in calcium, calcium organises Degree reaches 84.17%；Alternatively,
Spirulina in step 1) is continued to cultivate, temperature is adjusted to 30 DEG C, and light control exists in 4klx, pH control 10.5, external source iron FeSO47H is added into culture medium2O to final concentration 40mg/L is cultivated 3 days, obtains rich iron-containing spiral Algae, ferric organic degree reach 89.98%；Alternatively,
Spirulina in step 1) is continued to cultivate, temperature is adjusted to 30 DEG C, light control in 8klx, pH control 8.5, External source zinc ZnSO is added into culture medium4·7H2O to final concentration 40mg/L is cultivated 3 days, obtains the spirulina for being rich in zinc, and zinc has Machine degree reaches 58.28%；Alternatively,
Spirulina in step 1) is continued to cultivate, temperature is adjusted to 30 DEG C, and light control exists in 12klx, pH control 9.5, external source selenium Na is added into culture medium2SeO3It to final concentration 100mg/L, cultivates 5 days, obtains the spirulina for being rich in selenium, selenium has Machine degree reaches 70.54%.
Method above-mentioned, step 1) and 2) described in culture medium be ZarroukShi culture medium (Tables 1 and 2).
1 Zarrouk culture solution constituent of table
2 Zarrouk trace element solution constituent of table
Method above-mentioned, in step 1) after inoculation spirulina, the OD of algae solution560Value is 0.3.
In preceding method, the phosphorus acid for adjusting pH value for being 0.1mol/L with mass concentration, the spirulina is blunt top spirulina.
The present invention nutrient that external source is added into algae solution relative to spirulina be it is excessive, main purpose is to improve battalion The organic degree of element is supported, growth rate can be declined, but not influence its growth.
The present invention also provides the spirulinas for being rich in nutrient calcium, iron, zinc, selenium prepared by the above method.
The present invention further provides application of the above method in regulation nutritious spirulina element accumulation.
The present invention passes through the research environment factor (temperature, illumination, pH value) and spirulina absorption and accumulation calcium, iron, zinc and selenium member Element and its relationship of growth, the environmental condition of optimization nutritious spirulina element absorption accumulation, and then it is certain to reach spirulina cells Under the premise of increment, Accumulation of nutrient element is made to reach maximum.Pass through the regulation of environmental condition and external solution ion concentration The dual track system of regulation realizes the orientation accumulation of spirulina cells nutrient.This has important value in actual production.I.e. with Using different geographical, the variation of season environmental condition and photo-thermal combined breeding spirulina are come the orientation accumulation for realizing nutrient Target, Instructing manufacture practice produce the algae powder that Different Nutrient Elements content is high and its organic degree is high, make Spirulina Products Diversification, is not only greatly improved the economic benefit of enterprise, but also overcomes the defect of current nutrient supplement, pushes China's spiral The development of algae industry and health industry.
Method provided by the invention is substantially increased the content of four kinds of nutrients of ferro-calcium-zinc-selenium in spirulina, and Make nutrient in conjunction with intracellular organic matter, reduces to gastrointestinal irritation, improve absorption of human body utilization rate.Utilize this method The content of the calcium iron zinc of the spirulina cells of culture has been respectively increased 97%, 175% and 87% than traditional cultural method；Calcium iron zinc The organic degree of selenium is successively are as follows: 84.17%, 89.98%, 58.28% and 70.54%.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Unless otherwise specified, embodiment Used in the conventional means that are well known to those skilled in the art of technological means, raw materials used is commercial goods.
Embodiment 1
1) spirulina is inoculated into ZShi culture medium, after being inoculated with spirulina, the OD of algae solution560Value is 0.3.At 30 DEG C, It is cultivated 3 days under the condition of culture of 12klx, pH=9.5.
2) spirulina by culture 3 days in step 1) continues to cultivate, and temperature is adjusted to 20 DEG C, and light control is in 12klx, pH Control adds External Calcium CaCO into culture medium 8.53It to final concentration 0.6g/L, cultivates 3 days, obtains the spirulina for being rich in calcium (calcium element content is 2.52mg/g in spirulina).The organic degree of calcium is 53.57%.Growth of spirulina platensis rate K=0.0465.
Embodiment 2
1) spirulina is inoculated into ZShi culture medium, after being inoculated with spirulina, the OD of algae solution560Value is 0.3.At 30 DEG C, It is cultivated 3 days under the condition of culture of 12klx, pH=9.5.
2) spirulina by culture 3 days in step 1) continues to cultivate, and temperature is adjusted to 20 DEG C, and light control is in 12klx, pH Control adds External Calcium CaCO into culture medium 8.53It to final concentration 0.6g/L, cultivates 5 days, obtains the spirulina for being rich in calcium (calcium element content is 5.64mg/g in spirulina).The organic degree of calcium is 72.14%.Growth of spirulina platensis rate K=0.0448.
Embodiment 3
1) spirulina is inoculated into ZShi culture medium, after being inoculated with spirulina, the OD of algae solution560Value is 0.3.At 30 DEG C, It is cultivated 3 days under the condition of culture of 12klx, pH=9.5.
2) spirulina by culture 3 days in step 1) continues to cultivate, and temperature is adjusted to 20 DEG C, and light control is in 12klx, pH Control adds External Calcium CaCO into culture medium 8.53It to final concentration 0.6g/L, cultivates 7 days, obtains the spirulina for being rich in calcium (calcium element content is 18.21mg/g in spirulina).The organic degree of calcium is 84.17%.Growth of spirulina platensis rate K= 0.0411。
Embodiment 4
1) spirulina is inoculated into ZShi culture medium, after being inoculated with spirulina, the OD of algae solution560Value is 0.3.At 30 DEG C, It is cultivated 3 days under the condition of culture of 12klx, pH=9.5.
2) spirulina by the culture 3 days in step 1) continues to cultivate, and temperature is adjusted to 30 DEG C, light control in 4klx, PH is controlled 10.5, and external source iron FeSO is added into culture medium4·7H2O to final concentration 40mg/L is cultivated 7 days, is obtained and is rich in iron Spirulina (in spirulina iron content be 1538.26ppm).The organic degree of iron is 84.95%.Growth of spirulina platensis rate K=0.0332.
Embodiment 5
1) spirulina is inoculated into ZShi culture medium, after being inoculated with spirulina, the OD of algae solution560Value is 0.3.At 30 DEG C, It is cultivated 3 days under the condition of culture of 12klx, pH=9.5.
2) spirulina by culture 3 days in step 1) continues to cultivate, and temperature is adjusted to 30 DEG C, and light control is in 4klx, pH Control adds external source iron FeSO into culture medium 10.54·7H2O to final concentration 40mg/L is cultivated 3 days, is obtained rich iron-containing Spirulina (iron content is 1634.34ppm in spirulina).The organic degree of iron is 89.98%.Growth of spirulina platensis rate K =0.0411.
Embodiment 6
1) spirulina is inoculated into ZShi culture medium, after being inoculated with spirulina, the OD of algae solution560Value is 0.3.At 30 DEG C, It is cultivated 3 days under the condition of culture of 12klx, pH=9.5.
2) spirulina by culture 3 days in step 1) continues to cultivate, and temperature is adjusted to 30 DEG C, and light control is in 8klx, pH Control adds external source zinc ZnSO into culture medium 8.54·7H2O to final concentration 40mg/L is cultivated 7 days, obtains the spiral shell for being rich in zinc Revolve algae (Zn-ef ficiency content is 132.75ppm in spirulina).The organic degree of zinc is 5.34%.Growth of spirulina platensis rate K= 0.0376。
Embodiment 7
1) spirulina is inoculated into ZShi culture medium, after being inoculated with spirulina, the OD of algae solution560Value is 0.3.At 30 DEG C, It is cultivated 3 days under the condition of culture of 12klx, pH=9.5.
2) spirulina by culture 3 days in step 1) continues to cultivate, and temperature is adjusted to 30 DEG C, and light control is in 8klx, pH Control adds external source zinc ZnSO into culture medium 8.54·7H2O to final concentration 40mg/L is cultivated 3 days, obtains the spiral shell for being rich in zinc Revolve algae (Zn-ef ficiency content is 88.30ppm in spirulina).The organic degree of zinc is 58.28%.Growth of spirulina platensis rate K= 0.0429。
Embodiment 8
1) spirulina is inoculated into ZShi culture medium, after being inoculated with spirulina, the OD of algae solution560Value is 0.3.At 30 DEG C, It is cultivated 3 days under the condition of culture of 12klx, pH=9.5, reaches logarithmic growth phase.
2) spirulina by culture 3 days in step 1) continues to cultivate, and external source selenium Na is added into culture medium2SeO3It is dense to end It spends 100mg/L, cultivates 7 days, obtain the spirulina rich in selenium (selenium element content is 350.27ppm in spirulina).Selenium it is organic Change degree is 40.16%.Growth of spirulina platensis rate K=0.0246.
Embodiment 9
1) spirulina is inoculated into ZShi culture medium, after being inoculated with spirulina, the OD of algae solution560Value is 0.3.At 30 DEG C, It is cultivated 3 days under the condition of culture of 12klx, pH=9.5, reaches logarithmic growth phase.
2) spirulina by culture 3 days in step 1) continues to cultivate, and external source selenium Na is added into culture medium2SeO3It is dense to end It spends 100mg/L, cultivates 3 days, obtain the spirulina rich in selenium (selenium element content is 399.70ppm in spirulina).Selenium it is organic Change degree is 58.62%.Growth of spirulina platensis rate K=0.0277.
Embodiment 10
1) spirulina is inoculated into ZShi culture medium, after being inoculated with spirulina, the OD of algae solution560Value is 0.3.At 30 DEG C, It is cultivated 3 days under the condition of culture of 12klx, pH=9.5, reaches logarithmic growth phase.
2) spirulina by culture 3 days in step 1) continues to cultivate, and external source selenium Na is added into culture medium2SeO3It is dense to end It spends 100mg/L, cultivates 5 days, obtain the spirulina rich in selenium (selenium element content is 1011.00ppm in spirulina).Selenium it is organic Change degree is 70.54%.Growth of spirulina platensis rate K=0.0265.
Comparative example 1
1) spirulina is inoculated into ZShi culture medium, after being inoculated with spirulina, the OD of algae solution560Value is 0.3.At 30 DEG C, It is cultivated 3 days under the condition of culture of 4klx, pH=9.5.
2) spirulina by culture 3 days in step 1) continues to cultivate, and temperature is adjusted to 30 DEG C, and light control is in 4klx, pH Control is cultivated 4 days 9.5, and the accumulation of calcium is 244.66ppm.Growth of spirulina platensis rate K=0.0852.
Comparative example 2
1) spirulina is inoculated into ZShi culture medium, after being inoculated with spirulina, the OD of algae solution560Value is 0.3.At 40 DEG C, It is cultivated 3 days under the condition of culture of 8klx, pH=8.5.
2) spirulina by culture 3 days in step 1) continues to cultivate, and temperature is adjusted to 40 DEG C, and light control is in 8klx, pH Control is cultivated 4 days 8.5, and the accumulation of iron is 192.64ppm.Growth of spirulina platensis rate K=0.0804.
Comparative example 3
1) spirulina is inoculated into ZShi culture medium, after being inoculated with spirulina, the OD of algae solution560Value is 0.3.At 40 DEG C, It is cultivated 3 days under the condition of culture of 12klx, pH=9.5.
2) spirulina by culture 3 days in step 1) continues to cultivate, and temperature is adjusted to 40 DEG C, and light control is in 12klx, pH Control is cultivated 4 days 9.5, and the accumulation of zinc is 30.06ppm.Growth of spirulina platensis rate K=0.0944.
From the above results, method provided by the invention can greatly improve several nutrients of spirulina content and Organic degree improves absorption of human body utilization rate and provides effective means for reducing to GI irritation.
Although above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.

Claims (7)

1. a kind of method of regulation nutritious spirulina elemental iron element accumulation, which comprises the following steps:
1) spirulina is inoculated into culture medium, Yu Wendu 20-40 DEG C, illumination 4-12klx, is trained under conditions of pH value 8.5-10.5 It supports 3 days；
2) spirulina in step 1) is continued to train under conditions of 15-35 DEG C of temperature, illumination 2-20klx, pH value 8.5-11.0 It supports, by the external source addition ferro element into culture medium, cultivates 3-7 days, obtain the spirulina of high nutrition element.
2. the method according to claim 1, wherein step 2) is specific as follows: by the spirulina in step 1) after Continuous culture, temperature are adjusted to 25-35 DEG C, and light control is controlled in 2-6klx, pH in 10-11.0, and external source is added into culture medium FeSO4·7H2O to final concentration 30-60mg/L is cultivated 3-7 days, obtains rich iron-containing spirulina.
3. the method according to claim 1, wherein the following steps are included:
Spirulina in step 1) is continued to cultivate, temperature is adjusted to 30 DEG C, light control in 4klx, pH control 10.5, to External source iron FeSO47H is added in culture medium2O to final concentration 40mg/L is cultivated 3 days, obtains rich iron-containing spirulina.
4. the method according to claim 1, wherein step 1) and 2) described in culture medium be ZarroukShi train Support base.
5. according to the method described in claim 4, it is characterized in that, step 1) in inoculation spirulina after, the OD of algae solution560Value is 0.3。
6. method according to claim 1-5, which is characterized in that the phosphoric acid tune for being 0.1mol/L with mass concentration PH value is saved, the spirulina is blunt top spirulina.
7. the spirulina rich in nutrient iron of any one of -6 the method preparations according to claim 1.
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN87103457A (en) * 1987-05-13 1987-12-23 江西省农业科学院耕作栽培研究所 Spirulina technology of intensifying zinc and iron and product application
CN1144844A (en) * 1995-09-05 1997-03-12 中国科学院武汉植物研究所 Manufacturing method of iron-rich pure spirulina
DE202014006168U1 (en) * 2014-08-04 2014-10-09 Ökotec-Anlagenbau Gmbh Plant for rearing and reproduction of spirulina algae
CN106085943A (en) * 2016-08-04 2016-11-09 湖南新生活螺旋藻生物科技有限公司 A kind of Spirulina powder being enriched with rare element and its preparation method and application

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102517244B (en) * 2011-12-31 2014-02-19 北京林业大学 Method for improving metallothionein content of spirulina
CN102876579B (en) * 2012-08-03 2014-06-11 安徽大学 Method for culturing and detecting high selenium-enriched spirulina platensis
CN102925359A (en) * 2012-10-29 2013-02-13 中国科学院南海海洋研究所 Seawater spirulina selenium-rich culture method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN87103457A (en) * 1987-05-13 1987-12-23 江西省农业科学院耕作栽培研究所 Spirulina technology of intensifying zinc and iron and product application
CN1144844A (en) * 1995-09-05 1997-03-12 中国科学院武汉植物研究所 Manufacturing method of iron-rich pure spirulina
DE202014006168U1 (en) * 2014-08-04 2014-10-09 Ökotec-Anlagenbau Gmbh Plant for rearing and reproduction of spirulina algae
CN106085943A (en) * 2016-08-04 2016-11-09 湖南新生活螺旋藻生物科技有限公司 A kind of Spirulina powder being enriched with rare element and its preparation method and application

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
吕冰心等: "关键环境因子对螺旋藻营养元素生物积累及有机化程度的影响", 《食品工业科技》 *
张丽华: "日光温室养殖螺旋藻的温度光照条件及调控技术", 《贵州气象》 *
李日强等: "钝顶螺旋藻对五种元素生物富集作用的研究", 《山西大学学报》 *
江涓: "螺旋藻对铁的富集作用研究", 《湖北师范学院学报( 自然科学版)》 *
王龙等: "螺旋藻对微量元素的富集作用", 《食品研究与开发》 *
管华师编著: "《中华海洋本草 海洋药源微生物》", 30 September 2009 *
贾士儒主编: "《生物工程专业实验 第2版》", 30 September 2010 *

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