CN106085943A - A kind of Spirulina powder being enriched with rare element and its preparation method and application - Google Patents

A kind of Spirulina powder being enriched with rare element and its preparation method and application Download PDF

Info

Publication number
CN106085943A
CN106085943A CN201610646894.0A CN201610646894A CN106085943A CN 106085943 A CN106085943 A CN 106085943A CN 201610646894 A CN201610646894 A CN 201610646894A CN 106085943 A CN106085943 A CN 106085943A
Authority
CN
China
Prior art keywords
spirulina
rare element
algae
pond
iodine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201610646894.0A
Other languages
Chinese (zh)
Other versions
CN106085943B (en
Inventor
黎吉星
戴斌
陈必建
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hunan New Life Spirulina Biological Technology Co Ltd
Original Assignee
Hunan New Life Spirulina Biological Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hunan New Life Spirulina Biological Technology Co Ltd filed Critical Hunan New Life Spirulina Biological Technology Co Ltd
Priority to CN201610646894.0A priority Critical patent/CN106085943B/en
Publication of CN106085943A publication Critical patent/CN106085943A/en
Application granted granted Critical
Publication of CN106085943B publication Critical patent/CN106085943B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36Adaptation or attenuation of cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention proposes a kind of Spirulina powder being enriched with rare element and its preparation method and application, it is to add containing chelating agent and the culture fluid of rare element in spirulina culture pond, complex reaction is mainly passed through during making growth of spirulina platensis, coordinate self adsorption, the spirulina of post-directed training enrichment rare element, rare element exists with complex ion form in culture fluid, should after carrying out complexation after adding spirulina, spirulina is formed and stablizes and the complex molecules of complexity, rich iodine is produced with post-directed training, Fu Tie, zinc-rich, Fu Mei, the product of the special-purposes such as selenium-rich.The present invention is with low cost, and productivity is high, can produce to mcroorganism amount the protein of high-quality, full nutrition article, the most also discharge substantial amounts of oxygen simultaneously, can play the effect of environment purification.The advantages such as the active easy absorption of organic bio-iodine product salt prepared, high temperature resistant not loss, in the high temperature of 418 DEG C, the reserved of iodine is 93%, safe and reliable.

Description

A kind of Spirulina powder being enriched with rare element and its preparation method and application
Technical field
The present invention relates to life health care food technology field, be specifically related to a kind of Spirulina powder being enriched with rare element and Preparation method and application.
Background technology
Iodine actually only has 12-16 milligram in human body, and the overwhelming majority is stored in slight thyroid, and this point point Seeming inappreciable iodine but plays very important effect in the life process of human body, and it participates in manufacturing a class and is called first shape The material of glandular hormone, to the metabolism of whole human body, this material includes that the metabolism of hormone is sufficiently reflected.
The anemia of pregnant woman of iodine deficiency, the metabolism of fetus iodine is abnormal, easily causes miscarriage, premature labor;The fetal brain development of iodine deficiency is subject to To disturbing, there is a kind of sick sufferer of gram Ting Shi that is called, children's's cerebral hypoplasia, stupid, silly, slow-witted, mongolism, life after adult Work can not be taken care of oneself;The children physical development obstacle of iodine deficiency, IQ is low;The teenager of iodine deficiency, sexual organ development is slow, secondary Levy and be in infantile state;The adult of iodine deficiency, occur light, in, the goiter of severe, i.e. so-called " big neck bubble ", sexuality declines Move back and even lose, pachylosis, skin falling out etc..These situations are referred to as iodine deficiency, as the term suggests, it is simply that due to iodine deficiency Caused.
Nineteen ninety JIUYUE, in " children living, protect and develop Universal Declaration " of 71 states summit meetings signature, it is determined that Within 2000, the whole world realizes eliminating the target of iodine deficiency.In March, 1991, Chinese Government is word at declaration good sign, indicates China The solemn commitment of nation's quality improves in government.
Owing to iodine is a very active element, it is easy to volatilization.For ensureing Iodized salt quality, it is necessary to tightly pack, it is to avoid wind Blow, Exposure to Sunlight, the impact such as high temperature.At ordinary times should be iodine salt in the altar tank added a cover, never washing, pot are endured.Cooking is stewed soup and first to be descended dish After put salt, the quick-fried pot of iodine salt must not be used, in case iodine crosses heat sublimation.Lesion resident summarizes iodine salt disease preventing and treating with jingle Experience: " often eat iodine salt benefit many, the most anti-tumor of the most anti-moronism, keep properly volatilization prevention, cooking is stewed soup the most quick-fried pot ".
China is one of country that iodine deficiency harm is the most serious in the world, and former disease population reaches 4.78 hundred million, accounts for people from lesion, the world Mouthful 40%, account for Asia 60%, the existing Mentally deficiency of China reaches 10,170,000, wherein 80% is above causing due to iodine deficiency, The annual newly-increased intelligence disabled child child in the whole nation reaches 1,000,000 simultaneously.According to investigations, the child average IQ compared with normal child in iodine deficiency area is low 10-15 percentage point.For preventing the disease caused because of iodine deficiency, China announces annual May 5 and is set to whole nation iodine deficiency publicity Day.
China is in December, 1972, and State Council discloses " supplementation of iodine salt prevents and treats endemic goiter Tentative Measures ", respectively Ground achieves achievement highly visible in terms of controlling or eliminating iodine deficiency.But in recent years, due to Sal marketing management Confusion, Non-iodizedsalt district transprovincially writes off serious so that once obtaining the iodine deficiency bottom out again of basic controlling, some areas are very The biggest to rise amplitude.
Therefore, in China's regulation edible salt, it is necessary to add a certain amount of iodine, make a decision to eliminate iodine deficiency, national standard In GB14880, the content of regulation Iodine in Table Salt is 30mg/kg ± 20%.Owing to the iodine in the current Sal of China is inorganic iodine, no As organic iodine absorption rate is high, and the iodine in rich iodine spirulina is organic bio-iodine, and its distinguishing feature is that absorption of human body utilizes Rate is high, good health care effect.And, the Qualified iodized salt of China i.e. iodine adding amount in Sal is not therapeutic dose, but preventive dose, It it is the amount avoiding occurring iodine deficiency.Iodine is limited in the storage level of human body.Algae organic-biological iodine salt behaviour body is easily inhaled Receiving, be equivalent to the iodine number adding in Sal exponentially, unnecessary iodine can be excreted by urine, and therefore edible algae has Machine biology iodine salt is perfectly safe.
Summary of the invention
In view of this, the technical problem to be solved, it is simply that propose a kind of Spirulina powder being enriched with rare element And its preparation method and application.
For solving above-mentioned technical problem, the present invention is achieved by the following technical solutions:
A kind of Spirulina powder being enriched with rare element, it is to add containing chelating agent and rare unit in spirulina culture pond The culture fluid of element, main by complex reaction during making growth of spirulina platensis, coordinate self adsorption, post-directed training enrichment is dilute Having the spirulina of element, rare element exists with complex ion form in culture fluid, after carrying out complexation after adding spirulina Should, spirulina is formed the stable and complex molecules of complexity, thus trains the spirulina of enrichment rare element for a certain area, through after Phase processing obtains Spirulina powder.
Further, described chelating agent is ethylenediaminetetraacetic acid, described rare element include ferrum, magnesium, zinc, selenium, molybdenum, tungsten, Boron, manganese, titanium, chromium.
Further, described spirulina be through improved spirulina maxim I can being enriched with of I or Spirulina subaalsa or spirulina plalensis, accordingly, possibly together with I in described culture fluid.
A kind of preparation method of the Spirulina powder being enriched with rare element, it comprises the following steps:
(1) spirulina is selected
The trichome of spirulina body long 600-800 μm selected, screw diameter 50-60 μm, algal filament diameter 6-7 μm, spiral number 5-8 Individual;
(2) inoculation
When natural environment temperature rises to 15-20 DEG C, reach spirulina and start the primary condition of growth, spirulina is connect Kind cultivating to being enlarged in culturing pool, it is 0.2 that inoculum concentration reaches the algae solution OD value when 560nm wavelength in pond, and period is toward foster Grow the CO being passed through in pond needed for enough spirulinas produce2And sodium bicarbonate and carbamide, spirulina training liquid disclosure satisfy that daily output dry powder 8g/d.m2The demand of above yield, described culture fluid include the component of following concentration: 100-200g/100L sodium bicarbonate, The potassium dihydrogen phosphate of 30-100g/100L, 10-200g/100L sodium chloride, 30-100g/100L potassium sulfate, 30-100g/100L carbon Acid diamide, 30-100g/100L magnesium sulfate, 1-4g/100L calcium chloride, 1-4g/100L chelating agent, 1-6g/100L ferrous sulfate, 0.1-1g/100L zinc sulfate, 0-1g/100L sodium selenite and the selenium of each 0-1g/100L, molybdenum, tungsten, boron, manganese, titanium, chromium class are rare Element;
(3) algae is expanded
After cultivating 5-7 days, when algae solution OD value reaches 0.8 in pond, i.e. continue to expand algae in pond, expand algae solution OD value control after algae System, more than 0.2, can be gathered when the algae pond OD value expanding algae at first reaches more than 1.0;
(4) gather spirulina
Collecting time select when the morning 7 to 10 time carry out, by water pump extract algae solution, be collected by filtration into through inclined screen net Algae mud, more directly clean with tap water;
(5) sterilizing of algae mud is with dry
The algae mud gathered processes through pasteurization after entering workshop immediately, is the most quickly dried, is allowed to be formed Fine powdered product, through sterile manner processing and packing and storage;
(6) pack and store
After packing, storage uses raw material storage and the warehouse for finished product of overhead layer-stepping, it is ensured that ventilation in storehouse, dry, heap Put neat, place is dustless.
Further, in described step (1), described spirulina is for carrying out richness to I through I is improved The spirulina maxim of collection or Spirulina subaalsa or spirulina plalensis, accordingly, possibly together with the iodine of 0-4g/100L in described culture fluid Change potassium.
Further, described culture fluid includes the sodium bicarbonate of the component of following concentration: 170g/100L, 50g/100L Potassium dihydrogen phosphate, the sodium chloride of 100g/100L, the potassium sulfate of 50g/100L, the carbonyl diamine of 50g/100L, 50g/100L Magnesium sulfate, the calcium chloride of 3g/100L, the potassium iodide of 2g/100L, the chelating agent of 4g/100L, the ferrous sulfate of 4g/100L, 0.2g/100L obtain zinc sulfate, the sodium selenite of 0.2g/100L and the ferrum of each 0-1g/100L, magnesium, zinc, selenium, molybdenum, tungsten, boron, Manganese, titanium, chromium class rare element.
Further, a length of 30m of described culturing pool, pond width ratio is 3m, and the degree of depth is 60-70cm, and pond bottom sloping is 2 ‰, it is spaced 1m between culturing pool, described culturing pool is additionally provided with wheel type blender, blender rotating speed is 50 turns per minute, The condition of culture during algae solution flow velocity keeps 20m/min, and described culturing pool in pond is made to be: temperature 32 DEG C, intensity of illumination is 3-3.5 ten thousand Lux, pH value is 9.0-10.0.
Further, it is provided with divider wall, the long 1m of described divider wall, height in the pond of described culturing pool two ends distance pool side 60cm 50cm, in making pond, algae solution produces bumpy flow under blender stirs.
Further, described chelating agent is ethylenediaminetetraacetic acid.
The invention allows for by said method prepare enrichment rare element Spirulina powder in Sal Application, its addition in edible salt is 2-20 kg/tonne.
Compared with prior art, the device have the advantages that for: the present invention utilizes spirulina easily to adsorb and complexation Trace element, the concentration of its complexation is some cases greater than the characteristic of several orders of magnitude of storage in culture environment, and post-directed training is rich rare The Spirulina Products of element.The present invention is with low cost, and productivity is high, can produce to mcroorganism amount the protein of high-quality, and full price is sought Support article, the most also discharge substantial amounts of oxygen simultaneously, the effect of environment purification can be played.With the side of the present invention The advantages such as the active easy absorption of biological organic iodine product salt that method produces, high temperature resistant not loss, iodine in the high temperature of 418 DEG C Reserved is 93%, safe and reliable.
Detailed description of the invention
Get information about the present invention for allowing those skilled in the art become apparent from, the present invention will be made further below Explanation.
Embodiment 1
A kind of Spirulina subaalsa powder being enriched with rare element, it is to add containing chelating agent and dilute in spirulina culture pond There is the culture fluid of element, main by complex reaction during making growth of spirulina platensis, coordinate self adsorption, post-directed training richness The spirulina of collection rare element, rare element exists with complex ion form in culture fluid, carries out network after adding spirulina Answer after conjunction, formed in spirulina and stablize and the complex molecules of complexity, thus train the spirulina of enrichment rare element for a certain area, Postmenstruation, processing obtained Spirulina powder, and its concrete preparation method comprises the following steps:
(1) spirulina is selected
The Spirulina subaalsa filament long 600-800 μm selected, screw diameter 50-60 μm, algal filament diameter 6-7 μm, spiral Number 5-8;Therein
(2) inoculation
When natural environment temperature rises to 15-20 DEG C, reach spirulina and start the primary condition of growth, spirulina is connect Kind cultivating to being enlarged in culturing pool, it is 0.2 that inoculum concentration reaches the algae solution OD value when 560nm wavelength in pond, and period is toward foster Grow the CO being passed through in pond needed for enough spirulinas produce2And sodium bicarbonate and carbamide, spirulina training liquid disclosure satisfy that daily output dry powder 8g/d.m2The demand of above yield, described culture fluid include the component of following concentration: 100-200g/100L sodium bicarbonate, The potassium dihydrogen phosphate of 30-100g/100L, 10-200g/100L sodium chloride, 30-100g/100L potassium sulfate, 30-100g/100L carbon Acid diamide, 30-100g/100L magnesium sulfate, 1-4g/100L calcium chloride, 1-4g/100L chelating agent (ethylenediaminetetraacetic acid), 1-6g/ 100L ferrous sulfate, 0.1-1g/100L zinc sulfate, 0-1g/100L sodium selenite and the ferrum of each 0-1g/100L, magnesium, zinc, selenium, Molybdenum, tungsten, boron, manganese, titanium, chromium class rare element;
The a length of 30m of described culturing pool, pond width ratio is 3m, and the degree of depth is 60-70cm, and pond bottom sloping is 2 ‰, culturing pool Between be spaced 1m, described culturing pool is additionally provided with wheel type blender, blender rotating speed is 50 turns per minute, makes algae solution in pond Flow velocity keeps 20m/min;It is provided with divider wall, the long 1m of described divider wall, height in the pond of described culturing pool two ends distance pool side 60cm 50cm, in making pond, algae solution produces bumpy flow under blender stirs;In described culturing pool, condition of culture is: temperature 32 DEG C, illumination Intensity is 3-3.5 ten thousand lux, and pH value is 9.0-10.0;
(3) algae is expanded
After cultivating 5-7 days, when algae solution OD value reaches 0.8 in pond, i.e. continue to expand algae in pond, expand algae solution OD value control after algae System, more than 0.2, can be gathered when the algae pond OD value expanding algae at first reaches more than 1.0;
(4) gather spirulina
Collecting time select when the morning 7 to 10 time carry out, by water pump extract algae solution, be collected by filtration into through inclined screen net Algae mud, more directly clean with tap water;
(5) sterilizing of algae mud is with dry
The algae mud gathered processes through pasteurization after entering workshop immediately, is the most quickly dried, is allowed to be formed Fine powdered product, through sterile manner processing and packing and storage;
(6) pack and store
After packing, storage uses raw material storage and the warehouse for finished product of overhead layer-stepping, it is ensured that ventilation in storehouse, dry, heap Put neat, place is dustless.
In the present embodiment, according to training objective, optionally target rare element addition is increased to 1g/100L, Fu Tie, Fu Mei, zinc-rich, selenium-rich, rich molybdenum, rich tungsten, boron-rich, Fu Meng, Fu Tai, chromium-rich Spirulina powder can be trained out respectively for a certain area.
Daily management in incubation
Daily management is the important component part during spirulina high yielding culture, mainly includes three below aspect:
(1) spirulina breeding daily record is carried out.Record temperature, water temperature, weather conditions, rainfall, algae pond pH value, OD value frond Upgrowth situation, nutritional labeling monitoring situation, microscopy situation etc..
(2) stir in time.Stirring can make spirulina algae solution in uniform light in culture pond, gas exchange fully, nutrient salt Cloth is uniform.In fine, the high appropriate to the occasion many stirrings of temperature, stir number of times when rainy weather, temperature are low and can lack, the most every 30 Minute stirring once, each time rests in water flowing 2 circle and is advisable.
Embodiment 2
It is known that iodine has the strongest sterilizing function, people utilize this characteristic of iodine, and developing a lot is basic with iodine The disinfectant of element.And its essence of spirulina is exactly a kind of bacterium, it is called cyanobacteria in early days, iodine will be added in spirulina medium In, the growth to spirulina is a kind of huge disaster certainly, say, that this kind of spirulina to be cultivated becomes rich iodine spiral Algae is hardly possible.
Therefore, the present embodiment is with the difference of embodiment 1, and described spirulina is through I improved salt pool spiral Algae, I can be enriched with by it, and concrete mode is, spirulina under high temperature, high illumination, alkaline environment by fragmentation mode Breeding, allows spirulina be in this environment 1 year 365 day 24 hours every day, and keeps the nutrient of abundance, has done hundreds of group Carry out contrast test.Segmentation timesharing step by step, denier ground add I (every Sets of Measurement, the time is the most different).By nearly 5 Year, finally turning out the rich iodine spirulina algae kind that can reach requirement, the algae powder that it is produced is fully achieved national standard;This Outward, compare embodiment 1, described culture fluid adds the potassium iodide of 0-4g/100L, it is achieved the production mesh of rich iodine Spirulina powder Mark, also can be enriched with, in the richest iodine Spirulina powder, the rare element that other human bodies lack, and solves each department human body because lacking part Rare element threatens the problem that existence is healthy.Iodine and other rare elements are mainly complex reaction in the growth course of spirulina Journey, also having is absorption on a small quantity.Iodine and other rare elements exist with complex ion form in culture fluid at the beginning, are adding Answer after carrying out complexation after entering spirulina, form new, bigger, the most stable and the most complicated complex molecules.
It is further to note that spirulina is in addition to Spirulina subaalsa in the present invention, it is also possible to use spirulina plalensis Replacing with spirulina maxim, because after deliberation, the feature of spirulina maxim is fast growth, and the feature of Spirulina subaalsa is rich The ability of collection rare element is stronger.Thus spirulina can be prepared as the rich ferrum of the richest I, Fu Mei, zinc-rich, selenium-rich, richness Molybdenum, rich tungsten, boron-rich, Fu Meng, Fu Tai, chromium-rich Spirulina powder, it is also possible to be prepared as rich I selenium-rich simultaneously, rich molybdenum, rich tungsten, Boron-rich, Fu Meng, Fu Tai, chromium-rich Spirulina powder.
Embodiment 3
Owing to, in rich iodine SPIRULINA CULTIVATION, the addition of potassium iodide is the highest, on aspect impacts such as the speeds of growth of spirulina The biggest, therefore, the present embodiment is with the difference of embodiment 2, and the nutrition composition in culture fluid is the bicarbonate of 170g/100L Sodium, the potassium dihydrogen phosphate of 50g/100L, the sodium chloride of 100g/100L, the potassium sulfate of 50g/100L, the phosphinylidyne two of 50g/100L Amine, the magnesium sulfate of 50g/100L, the calcium chloride of 3g/100L, the potassium iodide of 2g/100L, the ethylenediaminetetraacetic acid of 4g/100L, 4g/ The ferrous sulfate of 100L, 0.2g/100L obtain zinc sulfate, the sodium selenite of 0.2g/100L and the ferrum of each 0-1g/100L, magnesium, Zinc, selenium, molybdenum, tungsten, boron, manganese, titanium, chromium class rare element, rare element adds according to the target that need to train for a certain area.
Embodiment 4
The Spirulina powder prepared is applied in Sal, and its addition in edible salt is 2-20 kg/tonne.
The rich iodine Spirulina powder product prepared through the method for embodiment 3 adds in edible salt, and addition is 5 kilograms/ Ton, the organic-biological iodine salt prepared is detected through Loudi City food and medicine inspection, the numbered SP20160217 of statement, Detect through BG/T13025.7-2012 detection method, record wherein content of iodine 25.7mg/kg, at the standard index of 21-39mg/kg In, the total arsenic in Spirulina powder product itself, cadmium, mercury content are detected, the numbered SP20160184 of statement, warp simultaneously GB5009.17-2014 method detects, mercury content 0.030mg/kg, less than the standard index of≤0.05mg/kg;Through GB5009.15- 2014 methods detections, cadmium content 0.10mg/kg, less than the standard index of≤0.2mg/kg;Detect through GB5009.11-2014 method, always Arsenic content 0.20mg/kg, less than the standard index of≤0.5mg/kg.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention Within god and principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.

Claims (10)

1. the Spirulina powder being enriched with rare element, it is characterised in that it is to add containing complexation in spirulina culture pond Agent and the culture fluid of rare element, mainly pass through complex reaction during making growth of spirulina platensis, coordinate self adsorption, orientation Cultivating the spirulina of enrichment rare element, rare element exists with complex ion form in culture fluid, after adding spirulina Answer after carrying out complexation, formed in spirulina and stablize and the complex molecules of complexity, thus train enrichment rare element for a certain area Spirulina, postmenstruation, processing obtained Spirulina powder.
The Spirulina powder of enrichment rare element the most according to claim 1, it is characterised in that described chelating agent is ethylenediamine Tetraacethyl, described rare element includes ferrum, magnesium, zinc, selenium, molybdenum, tungsten, boron, manganese, titanium, chromium.
The Spirulina powder of enrichment rare element the most according to claim 1, it is characterised in that described spirulina is through iodine unit Improved spirulina maxim I can being enriched with of element or Spirulina subaalsa or spirulina plalensis, accordingly, described Possibly together with I in culture fluid.
The preparation method of the Spirulina powder of enrichment rare element the most according to claim 1, it is characterised in that it include with Lower step:
(1) spirulina is selected
The trichome of spirulina body long 600-800 μm selected, screw diameter 50-60 μm, algal filament diameter 6-7 μm, spiral number 5-8;
(2) inoculation
When natural environment temperature rises to 15-20 DEG C, reach spirulina and start the primary condition of growth, spirulina is inoculated into Being enlarged in culturing pool cultivating, it is 0.2 that inoculum concentration reaches the algae solution OD value when 560nm wavelength in pond, and period is toward culturing pool In be passed through enough spirulinas produce needed for CO2And sodium bicarbonate and carbamide, spirulina training liquid disclosure satisfy that daily output dry powder 8g/ d.m2The demand of above yield, described culture fluid includes the sodium bicarbonate of the component of following concentration: 100-200g/100L, 30- The potassium dihydrogen phosphate of 100g/100L, 10-200g/100L sodium chloride, 30-100g/100L potassium sulfate, 30-100g/100L phosphinylidyne Diamidogen, 30-100g/100L magnesium sulfate, 1.4g/100L calcium chloride, 1-4g/100L chelating agent, 1-6g/100L ferrous sulfate, 0.1-1g/100L zinc sulfate, 0-1g/100L sodium selenite and the ferrum of each 0-1g/100L, magnesium, zinc, selenium, molybdenum, tungsten, boron, manganese, titanium, Chromium class rare element;
(3) algae is expanded
After cultivating 5-7 days, when in pond, algae solution OD value reaches 0.8, i.e. continuing to expand algae in pond, after expanding algae, algae solution OD value controls More than 0.2, can gather when the algae pond OD value expanding algae at first reaches more than 1.0;
(4) gather spirulina
Collecting time select when the morning 7 to 10 time carry out, by water pump extract algae solution, algae is collected by filtration into through inclined screen net Mud, more directly clean with tap water;
(5) sterilizing of algae mud is with dry
The algae mud gathered processes through pasteurization after entering workshop immediately, is the most quickly dried, and is allowed to be formed finely Powder product, through sterile manner processing and packing and storage;
(6) pack and store
After packing, storage uses the raw material storage of overhead layer-stepping and warehouse for finished product, it is ensured that ventilate in storehouse, be dried, stack whole Together, place is dustless.
Method the most according to claim 4, it is characterised in that in described step (1), described spirulina is through I Improved spirulina maxim I can being enriched with or Spirulina subaalsa or spirulina plalensis, accordingly, described training Possibly together with the potassium iodide of 0-4g/100L in nutrient solution.
Method the most according to claim 5, it is characterised in that described culture fluid includes the component of following concentration: 170g/ The sodium bicarbonate of 100L, the potassium dihydrogen phosphate of 50g/100L, the sodium chloride of 100g/100L, the potassium sulfate of 50g/100L, 50g/ The carbonyl diamine of 100L, the magnesium sulfate of 50g/100L, the calcium chloride of 3g/100L, the potassium iodide of 2g/100L, the complexation of 4g/100L Agent, the ferrous sulfate of 4g/100L, 0.2g/100L obtain zinc sulfate, the sodium selenite of 0.2g/100L and each 0-1g/100L Ferrum, magnesium, zinc, selenium, molybdenum, tungsten, boron, manganese, titanium, chromium class rare element.
Method the most according to claim 4, it is characterised in that a length of 30m of described culturing pool, pond width ratio is 3m, The degree of depth is 60-70cm, and pond bottom sloping is 2 ‰, is spaced 1m between culturing pool, and described culturing pool is additionally provided with wheel type stirring Machine, blender rotating speed is 50 turns per minute, makes the condition of culture during algae solution flow velocity keeps 20m/min, and described culturing pool in pond be: Temperature 32 DEG C, intensity of illumination is 3-3.5 ten thousand lux, and pH value is 9.0-10.0.
Method the most according to claim 7, it is characterised in that be provided with in the pond of described culturing pool two ends distance pool side 60cm Divider wall, the long 1m of described divider wall, high 50cm, in making pond, algae solution produces bumpy flow under blender stirs.
9. according to the method described in claim 4 or 5 or 6, it is characterised in that described chelating agent is ethylenediaminetetraacetic acid.
10. the Spirulina powder of the enrichment rare element prepared according to the method described in any one of claim 4-8 is at Sal In application, its addition in edible salt is 2-20 kg/tonne.
CN201610646894.0A 2016-08-04 2016-08-04 A kind of preparation method of the spirulina powder of enrichment iodine Active CN106085943B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610646894.0A CN106085943B (en) 2016-08-04 2016-08-04 A kind of preparation method of the spirulina powder of enrichment iodine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610646894.0A CN106085943B (en) 2016-08-04 2016-08-04 A kind of preparation method of the spirulina powder of enrichment iodine

Publications (2)

Publication Number Publication Date
CN106085943A true CN106085943A (en) 2016-11-09
CN106085943B CN106085943B (en) 2018-10-30

Family

ID=57457171

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610646894.0A Active CN106085943B (en) 2016-08-04 2016-08-04 A kind of preparation method of the spirulina powder of enrichment iodine

Country Status (1)

Country Link
CN (1) CN106085943B (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107156774A (en) * 2017-06-27 2017-09-15 益盐堂(应城)健康盐制盐有限公司 A kind of function salt with effect for reducing blood fat and preparation method thereof
CN110004103A (en) * 2016-12-31 2019-07-12 北京林业大学 A method of regulation nutritious spirulina elemental iron element accumulation
CN110257296A (en) * 2019-07-02 2019-09-20 浙江海洋大学 The cultural method and application thereof of chromium-rich spirulina
DE202022101508U1 (en) 2022-03-22 2022-04-11 Biswaranjan Acharya Spirulina production system with intelligent circuit

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105483062A (en) * 2016-02-02 2016-04-13 杭州优普西生物科技有限公司 Microelement-rich spirulina culture medium
CN105661366A (en) * 2016-01-08 2016-06-15 烟台布鲁拜尔生物制药有限公司 Preparation method of high-zinc spirulina powder

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105661366A (en) * 2016-01-08 2016-06-15 烟台布鲁拜尔生物制药有限公司 Preparation method of high-zinc spirulina powder
CN105483062A (en) * 2016-02-02 2016-04-13 杭州优普西生物科技有限公司 Microelement-rich spirulina culture medium

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Y. SINGH等: "Adaptation of a strain of Spirulina platensis to grow in cobalt- and iodine-enriched media", 《JOURNAL OF APPLIED BACTERIOLOGY》 *
杨心宁等: "利用螺旋藻富集碘的实验", 《台湾海峡》 *
高亚辉等: "海洋微藻胞外产物研究进展", 《MARINE SCIENCES》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110004103A (en) * 2016-12-31 2019-07-12 北京林业大学 A method of regulation nutritious spirulina elemental iron element accumulation
CN107156774A (en) * 2017-06-27 2017-09-15 益盐堂(应城)健康盐制盐有限公司 A kind of function salt with effect for reducing blood fat and preparation method thereof
CN110257296A (en) * 2019-07-02 2019-09-20 浙江海洋大学 The cultural method and application thereof of chromium-rich spirulina
DE202022101508U1 (en) 2022-03-22 2022-04-11 Biswaranjan Acharya Spirulina production system with intelligent circuit

Also Published As

Publication number Publication date
CN106085943B (en) 2018-10-30

Similar Documents

Publication Publication Date Title
CN106085943B (en) A kind of preparation method of the spirulina powder of enrichment iodine
CN103865812A (en) Greenhouse propagation method for arbuscular mycorrhizal fungus glomus versiforme microbial inoculum
Tzortzakis et al. Treated wastewater and fertigation applied for greenhouse tomato cultivation grown in municipal solid waste compost and soil mixtures
CN104542475B (en) Breeding method for experimental macaque
CN102487802A (en) Method for culturing selenium-enriched vegetables by alga selenium and polysaccharide water culture process
CN107466860A (en) A kind of Chinese yew FOS lactic bacteria activity beverage and preparation method thereof
CN100374009C (en) Method for breeding salt-tolerance mutant of sweet-potato
CN104031865A (en) Quick efficient nostoc sphaeroides breeding method
CN106811417A (en) A kind of culture medium and its cultural method for Alexandrium mimutum Halim
CN101019602B (en) Composite fertilizer for selenium-rich freshwater pearl
CN109652340A (en) A kind of soybean special-purpose multifunctional growth promoting bacteria agent and its preparation method and application
Salimova et al. CHLORELLA SUSPENSION PREPARATION TECHNOLOGY
CN105524838A (en) Method for two-stage culture of porphyridium by using urine as main nitrogen source
CN103880189B (en) Utilize the method that high temperature resistant cladocera purifies wawter bloom
Xu et al. Aquaponics production of wheatgrass (Triticum aestivum L.) in different horticultural substrates with African catfish (Clarias gariepinus) in northern Germany
CN107118969A (en) Mineral matter element and its cultural method of bioavilability in a kind of raising Dunaliella salina cell
CN103484402A (en) Bacillus subtilis X3 and application thereof for reducing generation of hydrogen sulfide in buffalo dung
CN112662583A (en) Improved culture medium suitable for vegetative growth of nostoc sphaeroides and preparation method thereof
CN104305444B (en) The production method of natural selenium-rich Succus Bambusae
CN104017732A (en) Culture medium and culture method for culturing cocconeis placentula by using wastewater of antibiotics plants
CN109438021A (en) A kind of organic selenium fertilizer and preparation method thereof
CN106636258A (en) Rice straw fermentation culture medium for generating vitamin B12 by rumen fluid, and method and application thereof
Cameron Fixation of nitrogen by algae and associated organisms in semi-arid soils; identification and characterization of soil organisms
JP2002126763A (en) Nephrite extraction substance, drinking water containing nephrite extraction substance, method for extracting nephrite extraction substance, and method for preparing drinking water containing nephrite extraction substance
Wilhelm Malnutrition in Laos

Legal Events

Date Code Title Description
DD01 Delivery of document by public notice

Addressee: Dai Bin

Document name: Notification of Acceptance of Patent Application

C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant