CN109438021A - A kind of organic selenium fertilizer and preparation method thereof - Google Patents
A kind of organic selenium fertilizer and preparation method thereof Download PDFInfo
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- CN109438021A CN109438021A CN201910017960.1A CN201910017960A CN109438021A CN 109438021 A CN109438021 A CN 109438021A CN 201910017960 A CN201910017960 A CN 201910017960A CN 109438021 A CN109438021 A CN 109438021A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
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- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The present invention provides a kind of organic selenium fertilizer and preparation method thereof, it is prepared by the component of following mass percent: distilled water 93.65%~93.8%, culture medium 0.4%~0.53%, strain 5.7%~5.8%, selenium source 0.06%~0.07%, culture medium is prepared by the component of following mass percent: magnesium sulfate 4.5%~6%, anhydrous calcium chloride 0.9%~2%, anhydrous sodium acetate 35%~40%, urea 10%~12.5%, potassium dihydrogen phosphate 14%~16.5%, dipotassium hydrogen phosphate 21%~25%, yeast extract 1.5%~3%, trace element solution 8%~13%, preparation method includes culture medium preparation, strain is added, selenium source is added, four steps are cultivated in sealing.Organic selenium fertilizer preparation method provided by the invention can effectively improve the Se content in fertilizer, so as to effectively improve the Se content of crops.
Description
Technical field
The present invention relates to fertilizer production preparation fields, and in particular to a kind of organic selenium fertilizer and preparation method thereof.
Background technique
Selenium is a kind of chemical element, is the beneficial nutrient of nutrient and plant necessary to animal body, and selenium is divided into again
Organic selenium and two kinds of inorganic selenium, organic selenium are easy to store in tissue, absorb, can be rapidly by human body benefit after being absorbed
With being effectively improved human body selenium deficiency situation.Selenium can not synthesize in human body, so to meet the needs of human body is to selenium, it is necessary to every
The balance of its Selenium Supplement, selenium concentration has important protective effect and facilitation to the physiological function of many organs, tissue, when
When selenium deficiency, human immunological competence's decline is just easily lead to, threatens human health and more than 40 kinds of diseases of life all
It is related with human body selenium deficiency, such as cancer, cardiovascular disease, hepatopathy, cataract, pancreatic disease, diabetes, genital system diseases disease
Shape.China is a selenium deficiency big country, and Chinese residents common practice is based on food plant property food, the main planting site of Grain in China
Northeast plain, Yangtze River Delta, the Delta of the Pearl River are low selenium area, to cause national selenium deficiency.Therefore raw in crops
Using selenium-rich fertilizer in growth process, the Se content in crop products can be effectively improved, there is weight for improving national selenium intake
Want meaning.
The application of Patent No. 201610669710.2 discloses a kind of method for preparing amino acid selenium using photosynthetic bacteria,
Including preparing culture medium, photosynthetic bacteria expand it is numerous, form amino acid solution plus selenium, form amino acid selenium solution, utilize this
The amino acid selenium solution that kind method obtains can be also used for the production process of Se-rich farm products directly as a kind of health care product
In.This application using sodium selenite as selenium source, not exclusively directly drink and can have an impact to human health, and deposits by inorganic selenium conversion
In the not high problem of selenium conversion ratio.
Summary of the invention
The present invention is to solve the problem of the low high production cost of organic selenium fertilizer conversion ratio in the prior art, and providing one kind has
Machine selenium fertilizer and preparation method thereof can effectively improve the conversion ratio of selenium in preparation process, improve the Se content in organic selenium fertilizer.
The present invention provides a kind of organic selenium fertilizer, is prepared by the component of following mass percent: distilled water 93.65%~
93.8%, culture medium 0.4%~0.53%, strain 5.7%~5.8%, selenium source 0.06%~0.07%；
Culture medium is prepared by the component of following mass percent: magnesium sulfate 4.5%~6%, anhydrous calcium chloride 0.9%
~2%, anhydrous sodium acetate 35%~40%, urea 10%~12.5%, potassium dihydrogen phosphate 14%~16.5%, dipotassium hydrogen phosphate
21%~25%, yeast extract 1.5%~3%, trace element solution 8%~13%.Organic selenium fertilizer provided by the invention is liquid
State, it is easier to be absorbed by plants, the nutrients such as nitrogen, phosphorus, potassium can also be provided for plant in addition to the Se content for increasing plant
And various trace elements, the culture formula with the prior art includes 100 kilograms of pure water, and 60~80g of magnesium sulfate, anhydrous chlorine is added
Change 0.5~3g of calcium, 50~70g of potassium dihydrogen phosphate, 35~50g of dipotassium hydrogen phosphate, 5~7g of yeast extract, 15~20g of urea, anhydrous
100~150g of sodium acetate is compared, and increases the content of sodium acetate, urea and dipotassium hydrogen phosphate, maintains the pH value of culture medium suitable
Photosynthetic bacterium growth is closed, while the content of carbon, nitrogen and phosphorus element needed for increasing photosynthetic bacterium growth, reduces magnesium sulfate and contain
Amount avoids excessive offer except the normal absorption for maintaining photosynthetic bacterium growth, is promoted using polynary catalytic way photosynthetic thin
Bacterium metabolism, to improve the conversion ratio of selenium.
The present invention provides a kind of organic selenium fertilizer, it is preferred that, trace element solution includes containing mass percentage
For the blue solution of 3E-5%~4E-5% zinc, 3E-6%~4E-6% copper and 2E-4%~3E-4% boric acid, contain 2E-
4%~4E-4% iron, 2E-5%~5E-5% cobalt, 4E-6%~7E-6% manganese element yellow solution, contain 2E-4%~4E-
4% biotin, 0.02%~0.04% p-aminobenzoic acid, 0.02%~0.04% niacin, 0.02%~0.04% hydrochloric acid
The colourless solution of thiamine.Trace element solution provides required various trace elements for the growth of photosynthetic bacteria, is added simultaneously
Multivitamin promotes the growth metabolism of photosynthetic bacteria with magnesium ion mating reaction.
The present invention provides a kind of organic selenium fertilizer, it is preferred that, in trace element solution, each component volume ratio is blue
Solution: yellow solution: colourless solution=1:1:3~5.
The present invention provides a kind of organic selenium fertilizer, it is preferred that, selenium source is sodium selenite, and selenium contains in every liter of organic selenium fertilizer
Amount is not less than 0.18g.In organic selenium fertilizer use process provided by the invention, fountain height is 1kg/ mus, relative to existing in the market
The effective content of organic selenium fertilizer significantly improves.
The present invention provides a kind of preparation method of organic selenium fertilizer, comprising the following steps:
S1, preparation of culture medium: at room temperature, be magnesium sulfate 4.5%~6%, anhydrous calcium chloride by mass percentage composition
0.9%~2%, anhydrous sodium acetate 35%~40%, urea 10%~12.5%, potassium dihydrogen phosphate 14%~16.5%, phosphoric acid
Hydrogen dipotassium 21%~25%, yeast extract 1.5%~3%, trace element solution 8%~13% culture sill be added distilled water
In, it stirs evenly；
S2, strain are added: strain being added in the culture medium of step S1, stirring；
S3, selenium source are added: selenium source is added after stirring 8~16h in the mixed liquor of step S2, removes the oxygen in mixed liquor
Gas；
S4, sealing are cultivated: mixed liquor being sealed and is cultivated, is 26 DEG C cultivating temperature, carries out illumination using 3 100W light bulbs
Under conditions of, it cultivates 20 days, organic selenium fertilizer can be obtained.Strain is added in culture sill after mixing, stirs after strain is added
It mixes uniformly, makes photosynthetic bacterium growth breeding a period of time, the concentration of photosynthetic bacteria can be to the conversion ratio of organic selenium when selenium source is added
Generation significantly affects, and the time of selenium source is added too early or can all influence final conversion ratio too late, the amount that selenium source is added equally can
Influence photosynthetic bacteria growth rate, the present invention in be added selenium source amount to the growth of photosynthetic bacteria without obvious retardation, together
When promote organic selenium conversion ratio effectively.
The present invention provides a kind of preparation method of organic selenium fertilizer, it is preferred that, it is used in step S3 and is passed through titanium dioxide
The mode of carbon removes oxygen.It is filled with enough carbon dioxide into the culture apparatus of strain and achievees the purpose that deoxygenation, is photosynthetic thin
The growth of bacterium provides suitable environment, and influence of this mode deoxygenation to cultivation temperature is lower, while compared to deoxygenation instrument and change
Deoxygenation is learned, deoxygenation cost is reduced.
The present invention provides a kind of preparation method of organic selenium fertilizer, it is preferred that, selenium source is sodium selenite in step S3,
Organic selenium conversion ratio is not less than 80% in organic selenium fertilizer.
The present invention provides a kind of preparation method of organic selenium fertilizer, it is preferred that, it is tieed up in the cultivating process of step S4
Stirring is held, stirring rate is 30~50 turns per minute.According to the extension of incubation time, bacterial concentration is gradually increased, can be gradually
Stirring rate is improved, stirring rate is too low to make strain sinking Local enrichment, influence conversion ratio, stirring rate is excessively high, generation
Shearing force may have an impact thallus, be unfavorable for thalli growth.
The preparation method of organic selenium fertilizer provided by the invention can effectively improve the conversion of organic selenium
Rate, conversion ratio reach as high as 90%, and based on the organic selenium fertilizer of this preparation, Se content is not less than in every liter of organic selenium fertilizer
0.18g can effectively improve plant selenium content.
Specific embodiment
Embodiment 1
A kind of organic selenium fertilizer is prepared by the component of following mass percent: distilled water 650L, culture medium 3500g, bacterium
Kind 40L, sodium selenite 450g；Organic selenium content is not less than 0.18g in every liter of organic selenium fertilizer；
Culture medium is prepared by the component of following mass percent: magnesium sulfate 164g, anhydrous calcium chloride 33g, anhydrous second
Sour sodium 1235g, urea 364g, potassium dihydrogen phosphate 495g, dipotassium hydrogen phosphate 754g, yeast extract 65g, trace element solution 390g.
It is 3.5E-5% zinc, 3.5E-6% copper and 2.5E-4% that trace element solution, which includes containing mass percentage,
The blue solution of boric acid, containing 3E-4% iron, 4.5E-5% cobalt, 5E-6% manganese element yellow solution, contain 3E-4% biology
The colourless solution of element, 0.03% p-aminobenzoic acid, 0.03% niacin, 0.035% thiamine hydrochloride.
In trace element solution, each component volume ratio is blue solution: yellow solution: colourless solution=1:1:4.
A kind of preparation method of organic selenium fertilizer, comprising the following steps:
S1, preparation of culture medium: at room temperature, by mass percentage composition be magnesium sulfate 4.7%, anhydrous calcium chloride 0.94%,
It is anhydrous sodium acetate 35.3%, urea 10.4%, potassium dihydrogen phosphate 14.1%, dipotassium hydrogen phosphate 22%, yeast extract 1.86%, micro
The culture sill of Element Solution 11% is added in distilled water, stirs evenly；
S2, strain are added: strain being added in the culture medium of step S1, stirring；
S3, selenium source are added: sodium selenite are added after stirring 8~16h in the mixed liquor of step S2, using being passed through titanium dioxide
The mode of carbon removes oxygen；Organic selenium conversion ratio is not less than 80% in organic selenium fertilizer；
S4, sealing are cultivated: mixed liquor being sealed and is cultivated, is 26 DEG C cultivating temperature, carries out illumination using 3 100W light bulbs
Under conditions of, stirring rate is 30~50 turns per minute, cultivates 20 days, organic selenium fertilizer can be obtained.
Total selenium (in terms of Se) in organic selenium fertilizer obtained in the present embodiment is 215mg/ml, organic selenium 196mg/ml,
Conversion ratio reaches 91%.
Embodiment 2
The present embodiment has different preparation components from embodiment 1.
A kind of organic selenium fertilizer is prepared by the component of following mass percent: distilled water 650L, culture medium 3675g, bacterium
Kind 39.54L, sodium selenite 416g；Organic selenium content is not less than 0.18g in every liter of organic selenium fertilizer；
Culture medium is prepared by the component of following mass percent: magnesium sulfate 220g, anhydrous calcium chloride 72g, anhydrous second
Sour sodium 1323g, urea 368g, potassium dihydrogen phosphate 515g, dipotassium hydrogen phosphate 809g, yeast extract 74g, trace element solution 294g.
It is 4E-5% zinc, 4E-6% copper and 3E-4% boric acid that trace element solution, which includes containing mass percentage,
Blue solution, containing 4E-4% iron, 4.5E-5% cobalt, 7E-6% manganese element yellow solution, containing 4E-4% biotin,
0.04% p-aminobenzoic acid, 0.04% niacin, 0.035% thiamine hydrochloride colourless solution.
In trace element solution, each component volume ratio is blue solution: yellow solution: colourless solution=1:1:4.
A kind of preparation method of organic selenium fertilizer, comprising the following steps:
S1, preparation of culture medium: at room temperature, be magnesium sulfate 6%, anhydrous calcium chloride 1.96%, nothing by mass percentage composition
Water sodium acetate 36%, urea 10%, potassium dihydrogen phosphate 14%, dipotassium hydrogen phosphate 22%, yeast extract 2%, trace element solution 8%
Culture sill be added distilled water in, stir evenly；
S2, strain are added: strain being added in the culture medium of step S1, stirring；
S3, selenium source are added: sodium selenite are added after stirring 8~16h in the mixed liquor of step S2, using being passed through titanium dioxide
The mode of carbon removes oxygen；Organic selenium conversion ratio is not less than 80% in organic selenium fertilizer；
S4, sealing are cultivated: mixed liquor being sealed and is cultivated, is 26 DEG C cultivating temperature, carries out illumination using 3 100W light bulbs
Under conditions of, stirring rate is 30~50 turns per minute, cultivates 20 days, organic selenium fertilizer can be obtained.
Total selenium (in terms of Se) in organic selenium fertilizer obtained in the present embodiment is 202mg/ml, organic selenium 186mg/ml,
Conversion ratio reaches 92%.
Embodiment 3
The present embodiment has different preparation components from embodiment 1.
A kind of organic selenium fertilizer is prepared by the component of following mass percent: distilled water 650L, culture medium 3675g, bacterium
Kind 39.85L, sodium selenite 485g；Organic selenium content is not less than 0.18g in every liter of organic selenium fertilizer；
Culture medium is prepared by the component of following mass percent: magnesium sulfate 184g, anhydrous calcium chloride 55g, anhydrous second
Sour sodium 1286g, urea 385g, potassium dihydrogen phosphate 535g, dipotassium hydrogen phosphate 790g, yeast extract 110g, trace element solution 330g.
It is 4E-5% zinc, 4E-6% copper and 3E-4% boric acid that trace element solution, which includes containing mass percentage,
Blue solution, containing 4E-4% iron, 5E-5% cobalt, 7E-6% manganese element yellow solution, contain 4E-4% biotin, 0.04%
P-aminobenzoic acid, 0.04% niacin, 0.04% thiamine hydrochloride colourless solution.
In trace element solution, each component volume ratio is blue solution: yellow solution: colourless solution=1:1:5.
A kind of preparation method of organic selenium fertilizer, comprising the following steps:
S1, preparation of culture medium: at room temperature, by mass percentage composition be magnesium sulfate 5%, anhydrous calcium chloride 1.5%, anhydrous
Sodium acetate 35%, urea 10.5%, potassium dihydrogen phosphate 14.7%, dipotassium hydrogen phosphate 21.5%, yeast extract 3%, microelement are molten
The culture sill of liquid 9% is added in distilled water, stirs evenly；
S2, strain are added: strain being added in the culture medium of step S1, stirring；
S3, selenium source are added: sodium selenite are added after stirring 8~16h in the mixed liquor of step S2, using being passed through titanium dioxide
The mode of carbon removes oxygen；Organic selenium conversion ratio is not less than 80% in organic selenium fertilizer；
S4, sealing are cultivated: mixed liquor being sealed and is cultivated, is 26 DEG C cultivating temperature, carries out illumination using 3 100W light bulbs
Under conditions of, stirring rate is 30~50 turns per minute, cultivates 20 days, organic selenium fertilizer can be obtained.
Total selenium (in terms of Se) in organic selenium fertilizer obtained in the present embodiment is 298mg/ml, organic selenium 254mg/ml,
Conversion ratio reaches 85%.
Embodiment 4
The present embodiment has different preparation components from embodiment 1.
A kind of organic selenium fertilizer is prepared by the component of following mass percent: distilled water 650L, culture medium 3050g, bacterium
Kind 39.5L, sodium selenite 416g；Organic selenium content is not less than 0.18g in every liter of organic selenium fertilizer；
Culture medium is prepared by the component of following mass percent: magnesium sulfate 140g, anhydrous calcium chloride 28g, anhydrous second
Sour sodium 1068g, urea 305g, potassium dihydrogen phosphate 427g, dipotassium hydrogen phosphate 640g, yeast extract 46g, trace element solution 396g.
It is 3E-5% zinc, 3E-6% copper and 2E-4% boric acid that trace element solution, which includes containing mass percentage,
Blue solution, containing 2E-4% iron, 2E-5% cobalt, 4E-6% manganese element yellow solution, contain 2E-4% biotin, 0.02%
P-aminobenzoic acid, 0.02% niacin, 0.02% thiamine hydrochloride colourless solution.
In trace element solution, each component volume ratio is blue solution: yellow solution: colourless solution=1:1:3.
A kind of preparation method of organic selenium fertilizer, comprising the following steps:
S1, preparation of culture medium: at room temperature, be magnesium sulfate 4.6%, anhydrous calcium chloride 0.9%, nothing by mass percentage composition
Water sodium acetate 35%, urea 10%, potassium dihydrogen phosphate 14%, dipotassium hydrogen phosphate 21%, yeast extract 1.5%, trace element solution
13% culture sill is added in distilled water, stirs evenly；
S2, strain are added: strain being added in the culture medium of step S1, stirring；
S3, selenium source are added: sodium selenite are added after stirring 8~16h in the mixed liquor of step S2, using being passed through titanium dioxide
The mode of carbon removes oxygen；Organic selenium conversion ratio is not less than 80% in organic selenium fertilizer；
S4, sealing are cultivated: mixed liquor being sealed and is cultivated, is 26 DEG C cultivating temperature, carries out illumination using 3 100W light bulbs
Under conditions of, stirring rate is 30~50 turns per minute, cultivates 20 days, organic selenium fertilizer can be obtained.
Embodiment 5
The present embodiment has different preparation components from embodiment 1.
A kind of organic selenium fertilizer is prepared by the component of following mass percent: distilled water 650L, culture medium 2775g, bacterium
Kind 39.7L, sodium selenite 485g；Organic selenium content is not less than 0.18g in every liter of organic selenium fertilizer；
Culture medium is prepared by the component of following mass percent: magnesium sulfate 125g, anhydrous calcium chloride 25g, anhydrous second
Sour sodium 1055g, urea 278g, potassium dihydrogen phosphate 390g, dipotassium hydrogen phosphate 635g, yeast extract 42g, trace element solution 225g.
It is 3.5E-5% zinc, 3.5E-6% copper and 2.5E-4% that trace element solution, which includes containing mass percentage,
The blue solution of boric acid, containing 3E-4% iron, 4E-5% cobalt, 6E-6% manganese element yellow solution, containing 3E-4% biotin,
0.02% p-aminobenzoic acid, 0.03% niacin, 0.04% thiamine hydrochloride colourless solution.
In trace element solution, each component volume ratio is blue solution: yellow solution: colourless solution=1:1:5.
A kind of preparation method of organic selenium fertilizer, comprising the following steps:
S1, preparation of culture medium: at room temperature, be magnesium sulfate 4.5%, anhydrous calcium chloride 0.9%, nothing by mass percentage composition
Water sodium acetate 38%, urea 10%, potassium dihydrogen phosphate 14%, dipotassium hydrogen phosphate 23%, yeast extract 1.5%, trace element solution
8.1% culture sill is added in distilled water, stirs evenly；
S2, strain are added: strain being added in the culture medium of step S1, stirring；
S3, selenium source are added: sodium selenite are added after stirring 8~16h in the mixed liquor of step S2, using being passed through titanium dioxide
The mode of carbon removes oxygen；Organic selenium conversion ratio is not less than 80% in organic selenium fertilizer；
S4, sealing are cultivated: mixed liquor being sealed and is cultivated, is 26 DEG C cultivating temperature, carries out illumination using 3 100W light bulbs
Under conditions of, stirring rate is 30~50 turns per minute, cultivates 20 days, organic selenium fertilizer can be obtained.
Embodiment 6
The present embodiment has different preparation components from embodiment 1.
A kind of organic selenium fertilizer is prepared by the component of following mass percent: distilled water 650L, culture medium 2775g, bacterium
Kind 39.7L, sodium selenite 485g；Organic selenium content is not less than 0.18g in every liter of organic selenium fertilizer；
Culture medium is prepared by the component of following mass percent: magnesium sulfate 125g, anhydrous calcium chloride 25g, anhydrous second
Sour sodium 1110g, urea 278g, potassium dihydrogen phosphate 390g, dipotassium hydrogen phosphate 583g, yeast extract 42g, trace element solution 222g.
It is 3.5E-5% zinc, 3.5E-6% copper and 2.5E-4% that trace element solution, which includes containing mass percentage,
The blue solution of boric acid, containing 3E-4% iron, 4E-5% cobalt, 6E-6% manganese element yellow solution, containing 3E-4% biotin,
0.02% p-aminobenzoic acid, 0.03% niacin, 0.04% thiamine hydrochloride colourless solution.
In trace element solution, each component volume ratio is blue solution: yellow solution: colourless solution=1:1:5.
A kind of preparation method of organic selenium fertilizer, comprising the following steps:
S1, preparation of culture medium: at room temperature, be magnesium sulfate 4.5%, anhydrous calcium chloride 0.9%, nothing by mass percentage composition
Water sodium acetate 40%, urea 10%, potassium dihydrogen phosphate 14%, dipotassium hydrogen phosphate 21%, yeast extract 1.5%, trace element solution
8% culture sill is added in distilled water, stirs evenly；
S2, strain are added: strain being added in the culture medium of step S1, stirring；
S3, selenium source are added: sodium selenite are added after stirring 8~16h in the mixed liquor of step S2, using being passed through titanium dioxide
The mode of carbon removes oxygen；Organic selenium conversion ratio is not less than 80% in organic selenium fertilizer；
S4, sealing are cultivated: mixed liquor being sealed and is cultivated, is 26 DEG C cultivating temperature, carries out illumination using 3 100W light bulbs
Under conditions of, stirring rate is 30~50 turns per minute, cultivates 20 days, organic selenium fertilizer can be obtained.
Embodiment 7
The present embodiment has different preparation components from embodiment 1.
A kind of organic selenium fertilizer is prepared by the component of following mass percent: distilled water 650L, culture medium 2775g, bacterium
Kind 39.7L, sodium selenite 485g；Organic selenium content is not less than 0.18g in every liter of organic selenium fertilizer；
Culture medium is prepared by the component of following mass percent: magnesium sulfate 125g, anhydrous calcium chloride 25g, anhydrous second
Sour sodium 974g, urea 347g, potassium dihydrogen phosphate 458g, dipotassium hydrogen phosphate 582g, yeast extract 42g, trace element solution 222g.
It is 3.5E-5% zinc, 3.5E-6% copper and 2.5E-4% that trace element solution, which includes containing mass percentage,
The blue solution of boric acid, containing 3E-4% iron, 4E-5% cobalt, 6E-6% manganese element yellow solution, containing 3E-4% biotin,
0.02% p-aminobenzoic acid, 0.03% niacin, 0.04% thiamine hydrochloride colourless solution.
In trace element solution, each component volume ratio is blue solution: yellow solution: colourless solution=1:1:5.
A kind of preparation method of organic selenium fertilizer, comprising the following steps:
S1, preparation of culture medium: at room temperature, be magnesium sulfate 4.5%, anhydrous calcium chloride 0.9%, nothing by mass percentage composition
Water sodium acetate 35%, urea 12.5%, potassium dihydrogen phosphate 16.5%, dipotassium hydrogen phosphate 21%, yeast extract 1.5%, microelement
The culture sill of solution 8% is added in distilled water, stirs evenly；
S2, strain are added: strain being added in the culture medium of step S1, stirring；
S3, selenium source are added: sodium selenite are added after stirring 8~16h in the mixed liquor of step S2, using being passed through titanium dioxide
The mode of carbon removes oxygen；Organic selenium conversion ratio is not less than 80% in organic selenium fertilizer；
S4, sealing are cultivated: mixed liquor being sealed and is cultivated, is 26 DEG C cultivating temperature, carries out illumination using 3 100W light bulbs
Under conditions of, stirring rate is 30~50 turns per minute, cultivates 20 days, organic selenium fertilizer can be obtained.
Embodiment 8
The present embodiment has different preparation components from embodiment 1.
A kind of organic selenium fertilizer is prepared by the component of following mass percent: distilled water 650L, culture medium 2775g, bacterium
Kind 39.7L, sodium selenite 485g；Organic selenium content is not less than 0.18g in every liter of organic selenium fertilizer；
Culture medium is prepared by the component of following mass percent: magnesium sulfate 125g, anhydrous calcium chloride 25g, anhydrous second
Sour sodium 1000g, urea 320g, potassium dihydrogen phosphate 430g, dipotassium hydrogen phosphate 611g, yeast extract 42g, trace element solution 222g.
It is 3.5E-5% zinc, 3.5E-6% copper and 2.5E-4% that trace element solution, which includes containing mass percentage,
The blue solution of boric acid, containing 3E-4% iron, 4E-5% cobalt, 6E-6% manganese element yellow solution, containing 3E-4% biotin,
0.02% p-aminobenzoic acid, 0.03% niacin, 0.04% thiamine hydrochloride colourless solution.
In trace element solution, each component volume ratio is blue solution: yellow solution: colourless solution=1:1:5.
A kind of preparation method of organic selenium fertilizer, comprising the following steps:
S1, preparation of culture medium: at room temperature, be magnesium sulfate 4.5%, anhydrous calcium chloride 0.9%, nothing by mass percentage composition
Water sodium acetate 36%, urea 11.5%, potassium dihydrogen phosphate 15.5%, dipotassium hydrogen phosphate 22%, yeast extract 1.5%, microelement
The culture sill of solution 8% is added in distilled water, stirs evenly；
S2, strain are added: strain being added in the culture medium of step S1, stirring；
S3, selenium source are added: sodium selenite are added after stirring 8~16h in the mixed liquor of step S2, using being passed through titanium dioxide
The mode of carbon removes oxygen；Organic selenium conversion ratio is not less than 80% in organic selenium fertilizer；
S4, sealing are cultivated: mixed liquor being sealed and is cultivated, is 26 DEG C cultivating temperature, carries out illumination using 3 100W light bulbs
Under conditions of, stirring rate is 30~50 turns per minute, cultivates 20 days, organic selenium fertilizer can be obtained.
Embodiment 9
The present embodiment has different preparation components from embodiment 1.
A kind of organic selenium fertilizer is prepared by the component of following mass percent: distilled water 650L, culture medium 2775g, bacterium
Kind 39.7L, sodium selenite 485g；Organic selenium content is not less than 0.18g in every liter of organic selenium fertilizer；
Culture medium is prepared by the component of following mass percent: magnesium sulfate 125g, anhydrous calcium chloride 25g, anhydrous second
Sour sodium 973g, urea 305g, potassium dihydrogen phosphate 388g, dipotassium hydrogen phosphate 695g, yeast extract 42g, trace element solution 222g.
It is 3.5E-5% zinc, 3.5E-6% copper and 2.5E-4% that trace element solution, which includes containing mass percentage,
The blue solution of boric acid, containing 3E-4% iron, 4E-5% cobalt, 6E-6% manganese element yellow solution, containing 3E-4% biotin,
0.02% p-aminobenzoic acid, 0.03% niacin, 0.04% thiamine hydrochloride colourless solution.
In trace element solution, each component volume ratio is blue solution: yellow solution: colourless solution=1:1:5.
A kind of preparation method of organic selenium fertilizer, comprising the following steps:
S1, preparation of culture medium: at room temperature, be magnesium sulfate 4.5%, anhydrous calcium chloride 0.9%, nothing by mass percentage composition
Water sodium acetate 35%, urea 11%, potassium dihydrogen phosphate 14%, dipotassium hydrogen phosphate 25%, yeast extract 1.5%, trace element solution
8% culture sill is added in distilled water, stirs evenly；
S2, strain are added: strain being added in the culture medium of step S1, stirring；
S3, selenium source are added: sodium selenite are added after stirring 8~16h in the mixed liquor of step S2, using being passed through titanium dioxide
The mode of carbon removes oxygen；Organic selenium conversion ratio is not less than 80% in organic selenium fertilizer.
Embodiment 10
The present embodiment has different preparation components from embodiment 1, illustrates as a comparison case.
A kind of organic selenium fertilizer is prepared by the component of following mass percent: distilled water 650L, culture medium 3445g, bacterium
Kind 39.85L, sodium selenite 485g；
Culture medium is prepared by the component of following mass percent: magnesium sulfate 184g, anhydrous calcium chloride 55g, anhydrous second
Sour sodium 1286g, urea 385g, potassium dihydrogen phosphate 535g, dipotassium hydrogen phosphate 790g, yeast extract 110g, trace element solution 100.
It is 4E-5% zinc, 4E-6% copper and 3E-4% boric acid that trace element solution, which includes containing mass percentage,
Blue solution, containing 4E-4% iron, 5E-5% cobalt, 7E-6% manganese element yellow solution, contain 4E-4% biotin, 0.04%
P-aminobenzoic acid, 0.04% niacin, 0.04% thiamine hydrochloride colourless solution.
In trace element solution, each component volume ratio is blue solution: yellow solution: colourless solution=1:1:3.
A kind of preparation method of organic selenium fertilizer, comprising the following steps:
S1, preparation of culture medium: at room temperature, be magnesium sulfate 5.3%, anhydrous calcium chloride 1.6%, nothing by mass percentage composition
It is water sodium acetate 37.3%, urea 10.3%, potassium dihydrogen phosphate 15.5%, dipotassium hydrogen phosphate 22.9%, yeast extract 3.2%, micro
The culture sill of Element Solution 2.9% is added in distilled water, stirs evenly；
S2, strain are added: strain being added in the culture medium of step S1, stirring；
S3, selenium source are added: sodium selenite are added after stirring 8~16h in the mixed liquor of step S2, using being passed through titanium dioxide
The mode of carbon removes oxygen；
S4, sealing are cultivated: mixed liquor being sealed and is cultivated, is 26 DEG C cultivating temperature, carries out illumination using 3 100W light bulbs
Under conditions of, stirring rate is 30~50 turns per minute, cultivates 20 days, organic selenium fertilizer can be obtained.
Total selenium (in terms of Se) in organic selenium fertilizer obtained in the present embodiment is 279mg/ml, organic selenium 203mg/ml,
Conversion ratio reaches 73%.
Described above to be merely exemplary for the purpose of the present invention, and not restrictive, those of ordinary skill in the art understand,
Without departing from the spirit and scope defined by the claims, any modification, the variation or equivalent that can be made, will all fall
Enter within protection scope of the present invention.
Claims (9)
1. a kind of organic selenium fertilizer, it is characterised in that: be prepared by the component of following mass percent: distilled water 93.65%~
93.8%, culture medium 0.4%~0.53%, strain 5.7%~5.8%, selenium source 0.06%~0.07%；
The culture medium is prepared by the component of following mass percent: magnesium sulfate 4.5%~6%, anhydrous calcium chloride 0.9%
~2%, anhydrous sodium acetate 35%~40%, urea 10%~12.5%, potassium dihydrogen phosphate 14%~16.5%, dipotassium hydrogen phosphate
21%~25%, yeast extract 1.5%~3%, trace element solution 8%~13%.
2. a kind of organic selenium fertilizer according to claim 1, it is characterised in that: the trace element solution includes containing quality
Percentage composition is that the blue of 3E-5%~4E-5% zinc, 3E-6%~4E-6% copper and 2E-4%~3E-4% boric acid is molten
Liquid, containing 2E-4%~4E-4% iron, 2E-5%~5E-5% cobalt, 4E-6%~7E-6% manganese element yellow solution, contain
2E-4%~4E-4% biotin, 0.02%~0.04% p-aminobenzoic acid, 0.02%~0.04% niacin, 0.02%~
The colourless solution of 0.04% thiamine hydrochloride.
3. a kind of organic selenium fertilizer according to claim 2, it is characterised in that: in the trace element solution, each group is seperated
Product is than being blue solution: yellow solution: colourless solution=1:1:3~5.
4. a kind of organic selenium fertilizer according to claim 1, it is characterised in that: the selenium source is sodium selenite.
5. a kind of organic selenium fertilizer according to claim 1, it is characterised in that: organic selenium content in every liter of organic selenium fertilizer
Not less than 0.18g.
6. a kind of preparation method of organic selenium fertilizer according to claim 1, it is characterised in that: the following steps are included:
S1, preparation of culture medium: at room temperature, by mass percentage composition be magnesium sulfate 4.5%~6%, anhydrous calcium chloride 0.9%~
2%, anhydrous sodium acetate 35%~40%, urea 10%~12.5%, potassium dihydrogen phosphate 14%~16.5%, dipotassium hydrogen phosphate
21%~25%, it in the culture sill addition distilled water of yeast extract 1.5%~3%, trace element solution 8%~13%, stirs
It mixes uniformly；
S2, strain are added: strain being added in the culture medium of step S1, stirring；
S3, selenium source are added: selenium source is added after stirring 8~16h in the mixed liquor of step S2, removes the oxygen in mixed liquor；
S4, sealing are cultivated: mixed liquor being sealed and is cultivated, is 26 DEG C cultivating temperature, the item of illumination is carried out using 3 100W light bulbs
Under part, cultivates 20 days, organic selenium fertilizer can be obtained.
7. a kind of preparation method of organic selenium fertilizer according to claim 6, it is characterised in that: used in step S3 and be passed through two
The mode of carbonoxide removes oxygen.
8. a kind of preparation method of organic selenium fertilizer according to claim 6, it is characterised in that: selenium source described in step S3 is
Sodium selenite, organic selenium conversion ratio is not less than 80% in the organic selenium fertilizer.
9. a kind of preparation method of organic selenium fertilizer according to claim 6, it is characterised in that: in the cultivating process of step S4
Middle maintenance stirring, stirring rate are 30~50 turns per minute.
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Effective date of registration: 20190604 Address after: Room 504, 25 Xinxin Garden, Suzhou Industrial Park, Jiangsu Province Applicant after: Gao Jianjun Address before: 215104 No. 15 Swan Dang Road, Wangshan Industrial Park, Wuzhong Development Zone, Suzhou City, Jiangsu Province Applicant before: SUZHOU HUANBAO AGRICULTURAL SCIENCE AND TECHNOLOGY Co.,Ltd. |
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