CN107445706A - A kind of organic Se-rich complex liquid and preparation method thereof and application method - Google Patents

A kind of organic Se-rich complex liquid and preparation method thereof and application method Download PDF

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CN107445706A
CN107445706A CN201710814913.0A CN201710814913A CN107445706A CN 107445706 A CN107445706 A CN 107445706A CN 201710814913 A CN201710814913 A CN 201710814913A CN 107445706 A CN107445706 A CN 107445706A
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organic
medium
complex liquid
mus
growth medium
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高建军
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Gao Jianjun
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SUZHOU HUANBAO AGRICULTURAL SCIENCE AND TECHNOLOGY Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention discloses a kind of organic Se-rich complex liquid, it is prepared by the component of following mass percent：Culture medium 72.0~79.9%, strain：20.0~27.9%, selenium source 0.01~0.1%；Wherein culture medium includes enriched medium, the first growth medium and the second growth medium；Wherein enriched medium includes distilled water, NH4Cl、NaHCO3、KH2PO4、CH3COONa、MgSO4·7H2O, NaCl, three growth factors and trace element solution；First growth medium includes sodium glutamate and propionic acid；Second growth medium is by including ammonium chloride, dipotassium hydrogen phosphate, magnesium chloride, sodium chloride and yeast extract；Its preparation method includes enriched medium, the first growth medium, the second growth medium, the preparation of culture medium, strain addition and selenium source and adds 6 steps.The present invention applies the Se content that crops can be effectively improved on crops, improves crop yield, reduces the residues of pesticides in crops.

Description

A kind of organic Se-rich complex liquid and preparation method thereof and application method
Technical field
The present invention relates to composite heat transfer manufacture and use field, and in particular to a kind of organic Se-rich complex liquid and its preparation Method and application method.
Background technology
Selenium is a kind of chemical element, and chemical symbol is Se, is a kind of nutrient beneficial to plant, and needed by human Trace element, selenium can improve human immunity, promote the propagation of lymphocyte and the synthesis of antibody and immunoglobulin.Selenium is to knot The kinds cancers such as intestinal cancer, cutaneum carcinoma, liver cancer, breast cancer have the function that obvious suppression and protection, its centre in body Metabolite methyl enol has stronger active anticancer.The nutrient such as selenium and vitamin E, allicin, linoleic acid, germanium, zinc has There is the oxidation resistant effect of collaboration, increase antioxidation activity.Meanwhile selenium has the function that to mitigate and alleviate heavy metal toxicity.
Human body selenium deficiency can cause a variety of diseases, most typically China Heilungkiang Keshan County endemic disease-Keshan disease, big bone It is female thin to save disease, cancer, angiocardiopathy, cataract, cyst fibre modification, hypertension, Thyroid Gland Swell, immune deficiency, lymph Born of the same parents' property anaemia, retinal flecks degeneration, muscular dystrophy, ulcerative colitis, arthritis and human body aging all with human body Selenium deficiency has direct contact.
Therefore, it is significant to health using selenium-enriched food.However, due to regional disparity, China's topsoil In Se content it is generally relatively low, average out to 0.2mg/kg, if having 25% by soil selenium-containing critical value, China of 0.127mg/kg Soil be in low selenium level.The crops of growth are planted in this soil, its selenium content is necessarily relatively low, can not meet human body Needed for health.
By adding Selenium-rich nutrient solution in the production process of crops, the selenium that can effectively improve crop products contains Amount, but the problems such as low utilization rate, influence quality of agricultural product be present in existing complex fertilizer product.
Application No. 201210107656.4 discloses a kind of Selenium-rich nutrient solution, by the raw material system of following weight Into：25~28 parts of sodium selenite, 12~15 parts of ammonium sulfate, 13~15 parts of anhydrous boric acid, 12~20 parts of potassium sulfate, clay 5~10 Part, 10~15 parts of Diammonium phosphate (DAP), 2~5 parts of fulvic acid, 0.5~1 part of zinc oxide, 0.5~1 part of sodium molybdate, manganese sulfate 0.5~1 Part.0.8~2 part of ferrous sulfate, 0.3~0.8 part of magnesium sulfate, 0.1~0.5 part of copper sulphate, 5~10 parts of humic acid, EM bacterium solutions 5~ 10 parts, 0.5~3 part of neopelex, 15~30 parts of thiophanate methyl, 0.6~2 part of salicylic acid, compound sodium nitrophenolate 1~6 Part, 40~50 parts of white carbon, 20~30 parts of ethylene glycol, 0.5~1 part of ETDA- sodium, 1~5 part of preservative, sodium carboxymethylcellulose 5 ~30 parts, 20~30 parts of OP emulsifying agents, water polishing is to 1000 parts.A great number of elements needed for growth can be provided for carambola, had Abundant trace element, organic acid and micropopulation, the medicament with sterilization prophylactic-therapeutic effect is also added into, can be same what is sprayed When play a part of prevent and treat pest and disease damage.
The content of the invention
The present invention be in order to overcome in the prior art complex fertilizer product during to crop products use exist utilize Rate is low, influences the problems such as quality of agricultural product, there is provided a kind of organic Se-rich complex liquid and preparation method thereof and application method, by right The improvement of product formula and application method, the Se content of crops can be effectively improved, improve crop yield, reduce farming Residues of pesticides in thing.
The present invention provides a kind of organic Se-rich complex liquid, is prepared by the component of following mass percent：Culture medium 72.0~79.9%, strain：20.0~27.9%, selenium source 0.01~0.1%；
Culture medium is prepared by the component of following mass percent：Enriched medium 99.50~99.79%, first is given birth to Long culture medium 0.01~0.03%, the second growth medium 0.20~0.49%；
Enriched medium is prepared by forming for following mass percent：Distilled water 96~98%, NH4Cl 0.8~ 1.2%th, NaHCO30.08~0.12, KH2PO40.01~0.03%, CH3COONa 0.2~0.3%, MgSO4·7H2O 0.01~0.03%, NaCl 0.08~0.11%, three growth factors 0.8~1.2%, trace element solution 0.8~1.2%；
First growth medium is prepared by forming for following mass percent：Sodium glutamate 40~60%, propionic acid 40 ~60%；
Second growth medium is prepared by forming for following mass percent：Ammonium chloride 23~30%, phosphoric acid hydrogen two Potassium 10~17%, magnesium chloride 3~7%, sodium chloride 47~58%, yeast extract 1~4%.
A kind of organic Se-rich complex liquid of the present invention, it is preferred that, selenium source is sodium selenate or sodium selenite.
A kind of organic Se-rich complex liquid of the present invention, it is preferred that, it is organic in every liter of organic Se-rich complex liquid The μ g of Se content >=200000.
The present invention further provides a kind of preparation method of organic Se-rich complex liquid, comprise the following steps：
S1, enriched medium preparation：At room temperature, by NH4Cl、NaHCO3、KH2PO4、CH3COONa、MgSO4· 7H2O, NaCl, three growth factors and trace element solution are added in pH=7.0 distilled water, are stirred, and obtain quality hundred It is distilled water 96~98%, NH to divide than composition4Cl 0.8~1.2%, NaHCO30.08~0.12%, KH2PO40.01~ 0.03%th, CH3COONa 0.2~0.3%, MgSO4·7H2O 0.01~0.03%, NaCl 0.08~0.11%, three growth because Son 0.8~1.2%, the enriched medium of trace element solution 0.8~1.2%；
S2, the first growth medium preparation：At room temperature, sodium glutamate and propionic acid are stirred, obtains quality hundred It is sodium glutamate 40~60%, the first growth medium of propionic acid 40~60% to divide than composition；
S3, the second growth medium preparation：At room temperature, by ammonium chloride, dipotassium hydrogen phosphate, magnesium chloride, sodium chloride, ferment Female cream stirs, obtain mass percent composition for ammonium chloride 23~30%, dipotassium hydrogen phosphate 10~17%, magnesium chloride 3~ 7%th, the second growth medium of sodium chloride 47~58%, yeast extract 1~4%；
S4, culture medium preparation：The first growth medium that enriched medium, the step S2 that step S1 is obtained are obtained and The second growth medium that step S3 is obtained, it is stirred at room temperature uniformly, it is enriched medium to obtain mass percent composition The culture medium of 99.50~99.79%, first growth medium 0.01~0.03%, the second growth medium 0.20~0.49%；
S5, strain add：The obtained culture mediums of step S4 and strain are added in clear water, mass ratio is clear water：Culture Base：Strain=94~96:3~5:1,15min is rocked every 5~6h at 25~30 DEG C, mixes 32~36h；
S6, selenium source add：The selenium source that mass ratio is 0.09~0.11% is added in step S5 mixed liquor, 25~30 15min is rocked every 5~6h at DEG C, is mixed 6~7 days, you can obtain organic Se-rich complex liquid.
Strain and selenium source branch are added, 32~36h of interval adds selenium source after strain addition, strain can be made to enter one Step activation, improve the conversion ratio of Organic Selenium.
The present invention further provides a kind of application method of organic Se-rich complex liquid on leafy vegetable, including following step Suddenly：
S1, at leafy vegetable growth period, carry out first time sprinkling, fountain height is 1kg/ mus；
S2,10~15 days before leafy vegetable harvesting when, carry out second and spray, fountain height is 1kg/ mus.
The present invention further provides a kind of application method of organic Se-rich complex liquid on melon-fruit-like vegetable, including following step Suddenly：
S1, at melon-fruit-like vegetable growth period, carry out first time sprinkling, fountain height is 1kg/ mus；
S2, before melon-fruit-like vegetable florescence, carry out second and spray, fountain height is 1kg/ mus；
S3, sprayed once after melon-fruit-like vegetable harvesting or every 15~20 days, fountain height is 1kg/ mus.
The present invention further provides a kind of application method of organic Se-rich complex liquid on rice, comprise the following steps：
S1, in rice plant of tillering stage, carry out first time sprinkling, fountain height is 1kg/ mus；
S2, in Rise's boot period, carry out second and spray, fountain height is 1kg/ mus；
S3, in rice milking stage early stage, carry out third time sprinkling, fountain height is 1kg/ mus.
The present invention further provides a kind of application method of organic Se-rich complex liquid on fruit tree, comprise the following steps：
S1, after blooming of fruit trees terminates, carry out first time sprinkling, fountain height is 1.5kg/ mus；
S2, interval carry out second and sprayed, fountain height is 1.5kg/ mus after 20~30 days.
The present invention further provides a kind of application method of organic Se-rich complex liquid on tea tree, comprise the following steps：
S1, tea tree teethe before 30~45 days, carry out first time sprinkling, fountain height is 1.5kg/ mus；
S2, interval carry out second and sprayed, fountain height is 1.5kg/ mus after 15~30 days.
Tea tree can carry out first time sprinkling in 45 days before budding, be spaced and carry out within 20~30 days spraying for second；Also can be Carry out first time sprinkling within 30 days before budding, be spaced and carry out within 15~20 days spraying for second.
Above by organic Se-rich complex liquid be used for leafy vegetable, melon-fruit-like vegetable, rice, fruit tree, tea tree sprinkling process Preferably in fine lower progress, it is impossible to raining, snow, carried out under the conditions of strong wind etc.；Simultaneously need to be in Pesticide use in sprinkling Carried out after at least 3 days.
The present invention can reach following effect：
A) Se content of crops is improved, sprinkling can once make per kilogram crops selenium content reach 60~70 micrograms.
B) crop yield is improved, can typically improve yield 5%~15%.
C) residues of pesticides of degraded crops.
Embodiment
Embodiment 1
A kind of organic Se-rich complex liquid, is prepared by the component of following mass percent：Culture medium 72.0~79.9%, Strain：20.0~27.9%, selenium source 0.01~0.1%；
Culture medium is prepared by the component of following mass percent：Enriched medium 99.50~99.79%, first is given birth to Long culture medium 0.01~0.03%, the second growth medium 0.20~0.49%；
Enriched medium is prepared by forming for following mass percent：Distilled water 96~98%, NH4Cl 0.8~ 1.2%th, NaHCO30.08~0.12%, KH2PO40.01~0.03%, CH3COONa 0.2~0.3%, MgSO4·7H2O 0.01~0.03%, NaCl 0.08~0.11%, three growth factors 0.8~1.2%, trace element solution 0.8~1.2%；
First growth medium is prepared by forming for following mass percent：Sodium glutamate 40~60%, propionic acid 40 ~60%；
Second growth medium is prepared by forming for following mass percent：Ammonium chloride 23~30%, phosphoric acid hydrogen two Potassium 10~17%, magnesium chloride 3~7%, sodium chloride 47~58%, yeast extract 1~4%.
Its preparation method, comprise the following steps：
S1, enriched medium preparation：At room temperature, into 10L, the distilled water that pH value is 7.0, NH is added4Cl： 100g；NaHCO3：10g；KH2PO4：2g；CH3COONa：25g；MgSO4·7H2O：2g；NaCl：10g；Three growth factors： 100ml；Trace element solution：100ml, stir, enriched medium is made；
S2, the first growth medium preparation：At room temperature, sodium glutamate 1.0g, propionic acid 1.0g are taken, is stirred It is even, obtain the first growth medium；
S3, the second growth medium preparation：At room temperature, ammonium chloride 10g, dipotassium hydrogen phosphate 5g, magnesium chloride 2g, chlorine are taken Change sodium 20g, yeast extract 1g, be uniformly mixed, obtain the second growth medium；
S4, culture medium preparation：The first growth medium that enriched medium, the step S2 that step S1 is obtained are obtained and The second growth medium that step S3 is obtained, it is stirred at room temperature uniformly, obtains culture medium；
S5, strain add：The obtained culture mediums of step S4 are poured into 1000L, pH value to be stirred in 7.0 clear water, 200L strains are added, rock 15min every 5~6h at 25~30 DEG C, mix 32~36h；
S6, selenium source add：100g selenium powders are added in step S5 mixed liquor, are rocked at 25~30 DEG C every 5~6h 15min, mix 6~7 days, you can obtain organic Se-rich complex liquid.
Embodiment 2
A kind of organic Se-rich complex liquid, is prepared by the component of following mass percent：Culture medium 72.0~79.9%, Strain：20.0~27.9%, selenium source 0.01~0.1%；
Culture medium is prepared by the component of following mass percent：Enriched medium 99.50~99.79%, first is given birth to Long culture medium 0.01~0.03%, the second growth medium 0.20~0.49%；
Enriched medium is prepared by forming for following mass percent：Distilled water 96~98%, NH4Cl 0.8~ 1.2%th, NaHCO30.08~0.12%, KH2PO40.01~0.03%, CH3COONa 0.2~0.3%, MgSO4·7H2O 0.01~0.03%, NaCl 0.08~0.11%, three growth factors 0.8~1.2%, trace element solution 0.8~1.2%；
First growth medium is prepared by forming for following mass percent：Sodium glutamate 40~60%, propionic acid 40 ~60%；
Second growth medium is prepared by forming for following mass percent：Ammonium chloride 23~30%, phosphoric acid hydrogen two Potassium 10~17%, magnesium chloride 3~7%, sodium chloride 47~58%, yeast extract 1~4%.
Its preparation method, comprise the following steps：
S1, enriched medium preparation：At room temperature, into 10L, the distilled water that pH value is 7.0, NH is added4Cl：95g； NaHCO3：10.5g；KH2PO4：1.5g；CH3COONa：26g；MgSO4·7H2O：1.5g；NaCl：11g；Three growth factors： 95ml；Trace element solution：105ml, stir, enriched medium is made；
S2, the first growth medium preparation：At room temperature, sodium glutamate 0.8g, propionic acid 1.2g are taken, is stirred It is even, obtain the first growth medium；
S3, the second growth medium preparation：At room temperature, ammonium chloride 9.5g, dipotassium hydrogen phosphate 6g, magnesium chloride are taken 1.5g, sodium chloride 21g, yeast extract 0.8g, it is uniformly mixed, obtains the second growth medium；
S4, culture medium preparation：The first growth medium that enriched medium, the step S2 that step S1 is obtained are obtained and The second growth medium that step S3 is obtained, it is stirred at room temperature uniformly, obtains culture medium；
S5, strain add：The obtained culture mediums of step S4 are poured into 1000L, pH value to be stirred in 7.0 clear water, 200L strains are added, rock 15min every 5~6h at 25~30 DEG C, mix 32~36h；
S6, selenium source add：100g selenium powders are added in step S5 mixed liquor, are rocked at 25~30 DEG C every 5~6h 15min, mix 6~7 days, you can obtain organic Se-rich complex liquid.
Embodiment 3
A kind of organic Se-rich complex liquid, is prepared by the component of following mass percent：Culture medium 72.0~79.9%, Strain：20.0~27.9%, selenium source 0.01~0.1%；
Culture medium is prepared by the component of following mass percent：Enriched medium 99.50~99.79%, first is given birth to Long culture medium 0.01~0.03%, the second growth medium 0.20~0.49%；
Enriched medium is prepared by forming for following mass percent：Distilled water 96~98%, NH4Cl 0.8~ 1.2%th, NaHCO30.08~0.12%, KH2PO40.01~0.03%, CH3COONa 0.2~0.3%, MgSO4·7H2O 0.01~0.03%, NaCl 0.08~0.11%, three growth factors 0.8~1.2%, trace element solution 0.8~1.2%；
First growth medium is prepared by forming for following mass percent：Sodium glutamate 40~60%, propionic acid 40 ~60%；
Second growth medium is prepared by forming for following mass percent：Ammonium chloride 23~30%, phosphoric acid hydrogen two Potassium 10~17%, magnesium chloride 3~7%, sodium chloride 47~58%, yeast extract 1~4%.
Its preparation method, comprise the following steps：
S1, enriched medium preparation：At room temperature, into 10L, the distilled water that pH value is 7.0, NH is added4Cl： 105g；NaHCO3：9.5g；KH2PO4：2.5g；CH3COONa：24g；MgSO4·7H2O：2.5g；NaCl：11g；Three growth factors： 105ml；Trace element solution：95ml, stir, enriched medium is made；
S2, the first growth medium preparation：At room temperature, sodium glutamate 1.2g, propionic acid 0.8g are taken, is stirred It is even, obtain the first growth medium；
S3, the second growth medium preparation：At room temperature, ammonium chloride 10.5g, dipotassium hydrogen phosphate 4g, magnesium chloride are taken 2.5g, sodium chloride 19g, yeast extract 1.2g, it is uniformly mixed, obtains the second growth medium；
S4, culture medium preparation：The first growth medium that enriched medium, the step S2 that step S1 is obtained are obtained and The second growth medium that step S3 is obtained, it is stirred at room temperature uniformly, obtains culture medium；
S5, strain add：The obtained culture mediums of step S4 are poured into 1000L, pH value to be stirred in 7.0 clear water, 200L strains are added, rock 15min every 5~6h at 25~30 DEG C, mix 32~36h；
S6, selenium source add：100g selenium powders are added in step S5 mixed liquor, are rocked at 25~30 DEG C every 5~6h 15min, mix 6~7 days, you can obtain organic Se-rich complex liquid.
Embodiment 4
Take pure water 95kg, the culture medium 3.5kg in embodiment 1, strain 1kg, sodium selenite 100g load container, rock Fusion, rocks fusion at 25 DEG C, rocks 10 minutes every 6 hours afterwards, be within 7 days can obtain the organic Se-rich complex liquid into Product, finished color salmon pink.Organic selenium content is 200,000 micrograms in every liter of organic Se-rich complex liquid.
Embodiment 5
Taking pure water 100kg, the culture medium 3.65kg in embodiment 1, strain 1.1kg, sodium selenite 110g load container, Fusion is rocked at 30 DEG C, was rocked afterwards every 6 hours 10 minutes, can obtain the organic Se-rich complex liquid finished product within 7 days, into Product color salmon pink.Organic selenium content is 250,000 micrograms in every liter of organic Se-rich complex liquid.
Embodiment 6
Take pure water 100kg, the culture medium 3.71kg in embodiment 1, strain 1.05kg, sodium selenite 105g, which load, to be held Device, fusion is rocked at 30 DEG C, rocked 15 minutes every 5 hours afterwards, can obtain within 8 days the organic Se-rich complex liquid into Product, finished color salmon pink.Every liter of bacterium solution is not less than 260,000 micrograms containing Organic Selenium.
Embodiment 7
Take pure water 100kg, the culture medium 3.65kg in embodiment 1, strain 1.02kg, sodium selenite 105g, which load, to be held Device, fusion is rocked at 25 DEG C, rocked 15 minutes every 5 hours afterwards, can obtain within 8 days the organic Se-rich complex liquid into Product, finished color salmon pink.Every liter of bacterium solution is not less than 240,000 micrograms containing Organic Selenium.
Embodiment 8
Take pure water 100kg, the culture medium 3.67kg in embodiment 1, strain 1.01kg, sodium selenite 103g, which load, to be held Device, fusion is rocked at 26 DEG C, rocked 13 minutes every 5 hours afterwards, can obtain within 8 days the organic Se-rich complex liquid into Product, finished color salmon pink.Every liter of bacterium solution is not less than 220,000 micrograms containing Organic Selenium.
Embodiment 9
Take pure water 100kg, the culture medium 3.68kg in embodiment 1, strain 1.03kg, sodium selenite 102g, which load, to be held Device, fusion is rocked at 28 DEG C, rocked 14 minutes every 5 hours afterwards, can obtain within 7 days the organic Se-rich complex liquid into Product, finished color salmon pink.Every liter of bacterium solution is not less than 230,000 micrograms containing Organic Selenium.
Embodiment 10
Take pure water 100kg, the culture medium 3.66kg in embodiment 1, strain 1.05kg, sodium selenite 104g, which load, to be held Device, fusion is rocked at 30 DEG C, rocked 18 minutes every 5.5 hours afterwards, can obtain within 7 days the organic Se-rich complex liquid into Product, finished color salmon pink.Every liter of bacterium solution is not less than 210,000 micrograms containing Organic Selenium.
Embodiment 11
Pure water 100kg, culture medium 3.65kg, strain 1.04kg are taken, sodium selenite 101g loads container, shaken at 27 DEG C Fusion is shaken, was rocked afterwards every 6 hours 15 minutes, can obtain the organic Se-rich complex liquid finished product within 8 days, finished color is orange Color.Every liter of bacterium solution is not less than 280,000 micrograms containing Organic Selenium.
Embodiment 12
A kind of application method of organic Se-rich complex liquid in green vegetables, comprises the following steps：
S1, when green vegetables transplanting starts long young leaves more than three, carry out first time sprinkling, fountain height is 1kg/ mus；
S2,10~15 days before green vegetables harvesting when, carry out second and spray, fountain height is 1kg/ mus.
The Se content that the present embodiment obtains green vegetables is 0.5~0.6mg/kg, can increase production 15% or so.The detection of Se content Method is with reference to GB5009.93-2010 (the first method).
Embodiment 13
The present invention further provides a kind of application method of organic Se-rich complex liquid on cucumber, comprise the following steps：
S1, in the cucumber growth phase, carry out first time sprinkling, fountain height is 1kg/ mus；
S2, before cucumber florescence, carry out second and spray, fountain height is 1kg/ mus；
S3, sprayed once after cucumber is plucked or every 15~20 days, fountain height is 1kg/ mus.
The Se content that the present embodiment obtains green vegetables is 0.2~0.5mg/kg, can increase production 5~25%.The detection of Se content Method is with reference to GB5009.93-2010 (the first method).
Embodiment 14
A kind of application method of organic Se-rich complex liquid in rice, comprises the following steps
S1, in rice plant of tillering stage, during wheat emergence more than 3cm, carry out first time sprinkling, fountain height is 1kg/ mus；
S2, during jointing, carry out second after Rise's boot period, the beginning of spring and spray, fountain height is 1kg/ mus；
S3, in rice milking stage early stage, carry out third time sprinkling, fountain height is 1kg/ mus.
The Se content that the present embodiment obtains green vegetables is 0.2~0.5mg/kg, can increase production 15~25%.The detection of Se content Method is with reference to GB5009.93-2010 (the first method).
Embodiment 15
The present invention further provides a kind of application method of organic Se-rich complex liquid on apple tree, comprise the following steps：
S1, after the apple tree florescence terminates, carry out first time sprinkling, fountain height is 1.5kg/ mus；
S2, interval carry out second and sprayed, fountain height is 1.5kg/ mus after 20~30 days.
The Se content that the present embodiment obtains green vegetables is 0.2~0.5mg/kg, can increase production 15~25%.The detection of Se content Method is with reference to GB5009.93-2010 (the first method).
Embodiment 16
The present invention further provides a kind of application method of organic Se-rich complex liquid on tea tree, comprise the following steps：
S1, tea tree teethe before 45 days, carry out first time sprinkling, fountain height is 1.5kg/ mus；
S2, interval carry out second and sprayed, fountain height is 1.5kg/ mus after 20~30 days.
The Se content that the present embodiment obtains green vegetables is 0.8~1.8mg/kg, can increase production 5~25%.The detection of Se content Method is with reference to GB5009.93-2010 (the first method).
Embodiment 17
The present invention further provides a kind of application method of organic Se-rich complex liquid on tea tree, comprise the following steps：
S1, tea tree teethe before 30 days, carry out first time sprinkling, fountain height is 1.5kg/ mus；
S2, interval carry out second and sprayed, fountain height is 1.5kg/ mus after 15~20 days.
The Se content that the present embodiment obtains green vegetables is 0.8~1.8mg/kg, can increase production 5~25%.The detection of Se content Method is with reference to GB5009.93-2010 (the first method).
It is described above to be merely exemplary for the purpose of the present invention, and it is nonrestrictive, and those of ordinary skill in the art understand, In the case where not departing from the spirit and scope that claim is limited, any modification, the change or equivalent that can make, will all fall Enter within protection scope of the present invention.

Claims (10)

  1. A kind of 1. organic Se-rich complex liquid, it is characterised in that：It is prepared by the component of following mass percent：Culture medium 72.0 ~79.9%, strain：20.0~27.9%, selenium source 0.01~0.1%；
    The culture medium is prepared by the component of following mass percent：Enriched medium 99.50~99.79%, first is given birth to Long culture medium 0.01~0.03%, the second growth medium 0.20~0.49%；
    The enriched medium is prepared by forming for following mass percent：Distilled water 96~98%, NH4Cl 0.8~ 1.2%th, NaHCO30.08~0.12%, KH2PO40.01~0.03%, CH3COONa 0.2~0.3%, MgSO4·7H2O 0.01~0.03%, NaCl 0.08~0.11%, three growth factors 0.8~1.2%, trace element solution 0.8~1.2%；
    First growth medium is prepared by forming for following mass percent：Sodium glutamate 40~60%, propionic acid 40 ~60%；
    Second growth medium is prepared by forming for following mass percent：Ammonium chloride 23~30%, phosphoric acid hydrogen two Potassium 10~17%, magnesium chloride 3~7%, sodium chloride 47~58%, yeast extract 1~4%.
  2. A kind of 2. organic Se-rich complex liquid according to claim 1, it is characterised in that：The selenium source is sodium selenate or sub- selenium Sour sodium.
  3. A kind of 3. organic Se-rich complex liquid according to claim 1, it is characterised in that：Have in every liter of organic Se-rich complex liquid The μ g of machine Se content >=200000.
  4. A kind of 4. preparation method of organic Se-rich complex liquid according to claim 1, it is characterised in that：Including following step Suddenly：
    S1, enriched medium preparation：At room temperature, by NH4Cl、NaHCO3、KH2PO4、CH3COONa、MgSO4·7H2O、 NaCl, three growth factors and trace element solution are added in pH=7.0 distilled water, are stirred, are obtained mass percent Form as distilled water 96~98%, NH4Cl 0.8~1.2%, NaHCO30.08~0.12, KH2PO40.01~0.03%, CH3COONa 0.2~0.3%, MgSO4·7H2O 0.01~0.03%, NaCl 0.08~0.11%, three growth factors 0.8~ 1.2%th, the enriched medium of trace element solution 0.8~1.2%；
    S2, the first growth medium preparation：At room temperature, sodium glutamate and propionic acid are stirred, obtains mass percent Form as the first growth medium of sodium glutamate 40~60%, propionic acid 40~60%；
    S3, the second growth medium preparation：At room temperature, by ammonium chloride, dipotassium hydrogen phosphate, magnesium chloride, sodium chloride, yeast extract Stir, obtain mass percent composition for ammonium chloride 23~30%, dipotassium hydrogen phosphate 10~17%, magnesium chloride 3~7%, Second growth medium of sodium chloride 47~58%, yeast extract 1~4%；
    S4, culture medium preparation：The first growth medium and step that enriched medium, the step S2 that step S1 is obtained are obtained The second growth medium that S3 is obtained, be stirred at room temperature uniformly, obtain mass percent composition for enriched medium 99.50~ 99.79%th, the first growth medium 0.01~0.03%, the culture medium of the second growth medium 0.20~0.49%；
    S5, strain add：The obtained culture mediums of step S4 and strain are added in clear water, mass ratio is clear water：Culture medium：Bacterium Kind=94~96:3~5:1,15min is rocked every 5~6h at 25~30 DEG C, mixes 32~36h；
    S6, selenium source add：The selenium source that mass ratio is 0.09~0.11% is added in step S5 mixed liquor, at 25~30 DEG C 15min is rocked every 5~6h, is mixed 6~7 days, you can obtain organic Se-rich complex liquid.
  5. A kind of 5. preparation method of organic Se-rich complex liquid according to claim 4, it is characterised in that：The selenium source is selenium Sour sodium or sodium selenite, the μ g of organic selenium content in every liter of organic Se-rich complex liquid >=200000.
  6. A kind of 6. application method of the organic Se-rich complex liquid according to claim 1 on leafy vegetable, it is characterised in that： Comprise the following steps：
    S1, at leafy vegetable growth period, carry out first time sprinkling, fountain height is 1kg/ mus；
    S2,10~15 days before leafy vegetable harvesting when, carry out second and spray, fountain height is 1kg/ mus.
  7. 7. a kind of application method of the organic Se-rich complex liquid according to claim 1 on melon-fruit-like vegetable, its feature exist In：Comprise the following steps：
    S1, at melon-fruit-like vegetable growth period, carry out first time sprinkling, fountain height is 1kg/ mus；
    S2, before melon-fruit-like vegetable florescence, carry out second and spray, fountain height is 1kg/ mus；
    S3, sprayed once after melon-fruit-like vegetable harvesting or every 15~20 days, fountain height is 1kg/ mus.
  8. A kind of 8. application method of the organic Se-rich complex liquid according to claim 1 on rice, it is characterised in that：Including Following steps：
    S1, in rice plant of tillering stage, carry out first time sprinkling, fountain height is 1kg/ mus；
    S2, in Rise's boot period, carry out second and spray, fountain height is 1kg/ mus；
    S3, in rice milking stage early stage, carry out third time sprinkling, fountain height is 1kg/ mus.
  9. A kind of 9. application method of the organic Se-rich complex liquid according to claim 1 on fruit tree, it is characterised in that：Including Following steps：
    S1, after blooming of fruit trees terminates, carry out first time sprinkling, fountain height is 1.5kg/ mus；
    S2, interval carry out second and sprayed, fountain height is 1.5kg/ mus after 20~30 days.
  10. A kind of 10. application method of the organic Se-rich complex liquid according to claim 1 on tea tree, it is characterised in that：Bag Include following steps：
    S1, tea tree teethe before 30~45 days, carry out first time sprinkling, fountain height is 1.5kg/ mus；
    S2, interval carry out second and sprayed, fountain height is 1.5kg/ mus after 15~30 days.
CN201710814913.0A 2017-09-12 2017-09-12 A kind of organic Se-rich complex liquid and preparation method thereof and application method Pending CN107445706A (en)

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CN108530143A (en) * 2018-05-07 2018-09-14 北京百丰天下生物科技有限公司 Ensure the biofluid bacterial manure and preparation method thereof of crop nutrition content equilibrium
CN109400241A (en) * 2019-01-09 2019-03-01 苏州寰宝农业科技有限公司 A kind of cultivation bucket and its use the method for preparing organic selenium fertilizer
CN109438021A (en) * 2019-01-09 2019-03-08 苏州寰宝农业科技有限公司 A kind of organic selenium fertilizer and preparation method thereof

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CN104844308A (en) * 2015-03-13 2015-08-19 高煜昕 Organic selenium-rich composite liquid and production method therefor
CN104838832A (en) * 2015-03-13 2015-08-19 高煜昕 Planting method of selenium-rich rice
CN104838833A (en) * 2015-03-13 2015-08-19 高煜昕 Planting method of selenium-rich leafy vegetables
CN104838834A (en) * 2015-03-13 2015-08-19 高煜昕 Planting method of selenium-rich melon and fruit vegetables

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CN104844308A (en) * 2015-03-13 2015-08-19 高煜昕 Organic selenium-rich composite liquid and production method therefor
CN104838832A (en) * 2015-03-13 2015-08-19 高煜昕 Planting method of selenium-rich rice
CN104838833A (en) * 2015-03-13 2015-08-19 高煜昕 Planting method of selenium-rich leafy vegetables
CN104838834A (en) * 2015-03-13 2015-08-19 高煜昕 Planting method of selenium-rich melon and fruit vegetables

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108530143A (en) * 2018-05-07 2018-09-14 北京百丰天下生物科技有限公司 Ensure the biofluid bacterial manure and preparation method thereof of crop nutrition content equilibrium
CN109400241A (en) * 2019-01-09 2019-03-01 苏州寰宝农业科技有限公司 A kind of cultivation bucket and its use the method for preparing organic selenium fertilizer
CN109438021A (en) * 2019-01-09 2019-03-08 苏州寰宝农业科技有限公司 A kind of organic selenium fertilizer and preparation method thereof

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