CN109400241A - A kind of cultivation bucket and its use the method for preparing organic selenium fertilizer - Google Patents
A kind of cultivation bucket and its use the method for preparing organic selenium fertilizer Download PDFInfo
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- CN109400241A CN109400241A CN201910017966.9A CN201910017966A CN109400241A CN 109400241 A CN109400241 A CN 109400241A CN 201910017966 A CN201910017966 A CN 201910017966A CN 109400241 A CN109400241 A CN 109400241A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/90—Apparatus therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention provides a kind of cultivation bucket, including staving (1), bung (2), it further include the feeding port (3) being arranged above staving (1), the U-shaped outlet (4) being arranged below staving (1), the lighting device (51) internal in transparent tube body (5) is arranged in transparent tube body (5), hairbrush (6), the agitating device (12) being arranged in the middle part of staving (1)；A kind of cultivation bucket provided by the invention using the method for preparing organic selenium fertilizer, including be added culture medium, strain is added, selenium source is added, deoxygenation operation, sealing are cultivated, discharge packing, are cleaned and dried.Cultivation bucket provided by the invention can provide suitable temperature, sufficient illumination and suitable flowing for the growth of photosynthetic bacteria, the industrialized production of matter at regular time and quantity is determined in realization, the method for preparing organic selenium fertilizer using it, the Se content in fertilizer can be effectively improved, to effectively improve the Se content of crops.
Description
Technical field
The present invention relates to fertilizer production preparation fields, and in particular to a kind of cultivation bucket and its uses the side for preparing organic selenium fertilizer
Method.
Background technique
Selenium is a kind of chemical element, is the beneficial nutrient of nutrient and plant necessary to animal body, and selenium is divided into again
Organic selenium and two kinds of inorganic selenium, organic selenium are easy to store in tissue, absorb, can be rapidly by human body benefit after being absorbed
With being effectively improved human body selenium deficiency situation.Selenium can not synthesize in human body, so to meet the needs of human body is to selenium, it is necessary to every
The balance of its Selenium Supplement, selenium concentration has important protective effect and facilitation to the physiological function of many organs, tissue, when
When selenium deficiency, human immunological competence's decline is just easily lead to, threatens human health and more than 40 kinds of diseases of life all
It is related with human body selenium deficiency, such as cancer, cardiovascular disease, hepatopathy, cataract, pancreatic disease, diabetes, genital system diseases disease
Shape.China is a selenium deficiency big country, and Chinese residents common practice is based on food plant property food, the main planting site of Grain in China
Northeast plain, Yangtze River Delta, the Delta of the Pearl River are low selenium area, to cause national selenium deficiency.Therefore raw in crops
Using selenium-rich fertilizer in growth process, the Se content in crop products can be effectively improved, there is weight for improving national selenium intake
Want meaning.
In the commercial process that organic selenium fertilizer is cultivated, need to provide suitable temperature, abundance for microbial fermentation
Illumination and suitable flowing, that realizes industrialized production determines matter at regular time and quantity, and traditional fermentor does not adapt to photosynthetic bacteria class
Microorganism growth needs.Photosynthetic bacteria has phototaxis, and in the fermenter for providing light environment, photosynthetic bacteria is liable to stick to luminous
Inner wall influences intensity of illumination.
Summary of the invention
The present invention is to provide a kind of training to solve the problem of the low high production cost of organic selenium fertilizer conversion ratio in the prior art
The method that bacterium bucket and its use prepare organic selenium fertilizer, can effectively improve the conversion ratio of selenium in preparation process, improve organic selenium fertilizer
In Se content, realize organic selenium fertilizer determine the industrialized production of matter at regular time and quantity.
The present invention provides a kind of cultivation bucket, including staving and the bung fixed with staving sealing, it is characterised in that: further include
Feeding port above staving is set, the U-shaped outlet below staving is set, the transparent tube body in the middle part of staving is set, is arranged
The hairbrush contacted on the outside of transparent tube body with transparent tube body is arranged in lighting device inside transparent tube body, is arranged in transparent pipe
The agitating device of body side is arranged on the outside of staving and is connected to the inlet pipeline inside staving, detergent line, CO2 pipeline, liquid
Position meter, temperature sensor.Lighting device is placed in transparent tube body avoid photosynthetic bacteria be attached on lighting device be difficult to it is clear
It removes, while making the uniform illumination in cultivation bucket；Conventional Portland glass, tempered glass, organic glass can be used in transparent tube body material
Glass or polycarbonate plastic etc..
The present invention provides a kind of cultivation bucket, it is preferred that, it is additionally provided on the outside of staving for realizing to cultivation bucket function
The control box that can control, for observing the observation window for accompanying bacterium situation in bucket.Monitoring device is set in cultivation bucket, while also being set
It is equipped with the control box of control various functions, is easy to implement the industrialized production of microculture, growth item needed for accurate control
Part realizes that timing is determined matter and quantitatively produced.
The present invention provides a kind of cultivation bucket, it is preferred that, U-shaped exit end is sewage draining exit, and one end is discharge gate.Row
Material mouth and sewage draining exit are provided separately, and are avoided pollution.
The present invention provides a kind of cultivation bucket, it is preferred that, hairbrush is fixedly connected with agitating device, by being arranged in bucket
The motor drive covered rotates jointly.The backboard and stirring blade of hairbrush are fixed together, and use a rotary supporting rod and motor
It is connected, saves installation space, while the rotation of hairbrush can equally play the role of stirring, improves stirring efficiency.
The present invention provides a kind of cultivation bucket, it is preferred that, lighting device is along the 3 of the radially uniform setting of transparent tube body
A 100W light bulb.Lighting device is set according to the volume of cultivation bucket, provides suitable intensity of illumination for the growth of photosynthetic bacteria, is shone
Bright device is arranged radially, and is conducive to make the uniform illumination in cultivation bucket.
The present invention provides a kind of method that the use of cultivation bucket prepares organic selenium fertilizer, comprising the following steps:
S1, culture medium is added: by inlet pipeline, distilled water is added into cultivation bucket, feeding port is opened, into cultivation bucket
Culture sill is added, agitating device is started by control box, stirring rate is set as 30 revs/min, and culture sill is stirred
It mixes uniformly；
S2, strain is added: maintains stirring, strain is added by feeding port；
S3, selenium source is added: sodium selenite is added after stirring 8~16h in step S2；
S4, deoxygenation operation: closing feeding port, is filled with CO2 into bucket by CO2 pipeline, oxygen in bucket is discharged；
S5, sealing are cultivated: cultivation temperature is set 26 DEG C by setting, opens lighting device, closed to cultivate 20 days；
S6, discharge packing: opening discharge gate, will cultivate the organic selenium fertilizer completed and is discharged, encapsulating packing；
S7, cleaning-drying: by detergent line, clear water is added into cultivation bucket and is cleaned, it is dirty to open sewage draining exit discharge
Water repeats 3~4 times, opens bung and is dried, fixed bung after the completion of dry, in case next time uses.
The present invention provides a kind of method that the use of cultivation bucket prepares organic selenium fertilizer, it is preferred that, in step s 5, training
Stirring rate is adjusted to 50 revs/min after educating 10~15 days.According to the extension of incubation time, bacterial concentration is gradually increased, can
To step up stirring rate, stirring rate is too low to make strain sinking Local enrichment, influence conversion ratio, and stirring rate is excessively high,
The shearing force of generation may have an impact thallus, be unfavorable for thalli growth.
The present invention provides a kind of cultivation bucket using the method for preparing organic selenium fertilizer, it is preferred that, organic selenium fertilizer by with
The component of lower mass percent is prepared: distilled water 93.65%~93.8%, culture medium 0.4%~0.53%, strain
5.7%~5.8%, selenium source 0.06%~0.07%.When the amount of addition selenium source is reduced to 60%, the conversion ratio of organic selenium be can be improved
To 96%.
The present invention provides a kind of method that the use of cultivation bucket prepares organic selenium fertilizer, it is preferred that, culture medium is by following
The component of mass percent is prepared: magnesium sulfate 4.5%~6%, anhydrous calcium chloride 0.9%~2%, anhydrous sodium acetate 35%
~40%, urea 10%~12.5%, potassium dihydrogen phosphate 14%~16.5%, dipotassium hydrogen phosphate 21%~25%, yeast extract
1.5%~3%, trace element solution 8%~13%.
The present invention provides a kind of method that the use of cultivation bucket prepares organic selenium fertilizer, it is preferred that, trace element solution
Including being 3E-5%~4E-5% zinc, 3E-6%~4E-6% copper and 2E-4%~3E-4% boron containing mass percentage
The blue solution of acid, the yellow containing 2E-4%~4E-4% iron, 2E-5%~5E-5% cobalt, 4E-6%~7E-6% manganese element
Solution contains 2E-4%~4E-4% biotin, 0.02%~0.04% p-aminobenzoic acid, 0.02%~0.04% Buddhist nun gram
The colourless solution of acid, 0.02%~0.04% thiamine hydrochloride, each component volume ratio are blue solution: yellow solution: colourless molten
Liquid=1:1:4.Present invention optimizes the ratios between the proportion of photosynthetic bacteria culture medium and culture medium, strain, selenium source, and
The optimal time that selenium source is added has been explored, the organic selenium fertilizer that every liter of organic selenium content is not less than 0.18g has been produced, has made organic
The conversion ratio of selenium is not less than 80%.
Cultivation bucket provided by the invention is provided with transparent tube body in staving, and transparent tank interior is provided with lighting device.It will
Lighting device, which is placed in transparent tube body, to be avoided photosynthetic bacteria from being attached on lighting device being difficult to clean off, while being made in cultivation bucket
Uniform illumination.
The backboard of hairbrush and stirring blade will further be fixed together by cultivation bucket provided by the invention, and it is empty to save installation
Between, improve stirring efficiency.
Cultivation bucket provided by the invention can effectively improve the conversion ratio of organic selenium using the method for preparing organic selenium fertilizer,
Conversion ratio reaches as high as 90%, and based on the organic selenium fertilizer of this preparation, Se content is not less than 0.18g in every liter of organic selenium fertilizer, can be with
Effectively improve plant selenium content.
Detailed description of the invention
Fig. 1 is a kind of perspective view of cultivation bucket；
Fig. 2 is a kind of sectional view of cultivation bucket；
Appended drawing reference: 1, staving；2, bung；3, feeding port；4, U-shaped outlet；41, sewage draining exit；42, discharge gate；5, transparent
Tube body；6, hairbrush；7, inlet pipeline；8, detergent line；9, CO2 pipeline；10, liquidometer；11, temperature sensor；12, stirring dress
It sets；13, control box；14, observation window；15, motor.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
Embodiment 1
A kind of cultivation bucket seals fixed bung 2 including staving 1 and with staving 1, further includes that 1 top of staving is arranged in
The U-shaped outlet 4 below staving 1 is arranged in feeding port 3, and 3 one end of U-shaped outlet are sewage draining exit 41, and one end is discharge gate 42, setting
Transparent tube body 5 at 1 middle part of staving, is arranged in the lighting device 51 inside transparent tube body 5, and lighting device is along transparent tube body 4
3 100W light bulbs of radially uniform setting are arranged in the hairbrush 6 that 5 outside of transparent tube body is contacted with transparent tube body 5, are arranged saturating
The agitating device 12 of bright 5 side of tube body, hairbrush 6 are fixedly connected with agitating device 12, pass through 15 band of motor being arranged on bung 1
Inlet pipeline 7, detergent line 8, CO2 pipeline 9, liquid dynamic to rotate jointly, that 1 outside of staving is set and is connected to inside staving 1
Position meter 10, temperature sensor 11, be additionally provided on the outside of staving 1 for realizing to cultivation bucket function control control box 13, be used for
The observation window 14 of bacterium situation is accompanied in observation bucket.
A kind of method that the use of cultivation bucket prepares organic selenium fertilizer, comprising the following steps:
S1, culture medium is added: by inlet pipeline, distilled water 650L is added into cultivation bucket, feeding port is opened, to cultivation
Culture sill 3500g is added in bucket, agitating device is started by control box, stirring rate is set as 30 revs/min, will cultivate
Sill stirs evenly；Culture medium is prepared by the component of following mass percent: magnesium sulfate 164g, anhydrous calcium chloride 33g,
Anhydrous sodium acetate 1235g, urea 364g, potassium dihydrogen phosphate 495g, dipotassium hydrogen phosphate 754g, yeast extract 65g, trace element solution
390g, it is 3.5E-5% zinc, 3.5E-6% copper and 2.5E-4% boron that trace element solution, which includes containing mass percentage,
Acid blue solution, containing 3E-4% iron, 4.5E-5% cobalt, 5E-6% manganese element yellow solution, containing 3E-4% biotin,
0.03% p-aminobenzoic acid, 0.03% niacin, 0.035% thiamine hydrochloride colourless solution, blue solution: yellow is molten
Liquid: colourless solution=1:1:4.
S2, strain is added: maintains stirring, strain 40L is added by feeding port；
S3, selenium source is added: sodium selenite 450g is added after stirring 8~16h in step S2；
S4, deoxygenation operation: closing feeding port, is filled with CO2 into bucket by CO2 pipeline, oxygen in bucket is discharged；
S5, sealing are cultivated: cultivation temperature is set 26 DEG C by setting, opens lighting device, closed to cultivate 20 days；Cultivate 10
Stirring rate is adjusted to 50 revs/min after~15 days；
S6, discharge packing: opening discharge gate, will cultivate the organic selenium fertilizer completed and is discharged, encapsulating packing；
S7, cleaning-drying: by detergent line, clear water is added into cultivation bucket and is cleaned, it is dirty to open sewage draining exit discharge
Water repeats 3~4 times, opens bung and is dried, fixed bung after the completion of dry, in case next time uses.
Total selenium (in terms of Se) in organic selenium fertilizer obtained in the present embodiment is 215mg/ml, organic selenium 196mg/ml,
Conversion ratio reaches 91%.
Embodiment 2
The present embodiment from embodiment 1 there are different preparations to form.
A kind of method that the use of cultivation bucket prepares organic selenium fertilizer, comprising the following steps:
S1, culture medium is added: by inlet pipeline, distilled water 650L is added into cultivation bucket, feeding port is opened, to cultivation
Culture sill 3675g is added in bucket, agitating device is started by control box, stirring rate is set as 30 revs/min, will cultivate
Sill stirs evenly；Culture medium is prepared by the component of following mass percent: magnesium sulfate 220g, anhydrous calcium chloride 72g,
Anhydrous sodium acetate 1323g, urea 368g, potassium dihydrogen phosphate 515g, dipotassium hydrogen phosphate 809g, yeast extract 74g, trace element solution
294g, it is 4E-5% zinc that trace element solution, which includes containing mass percentage, the indigo plant of 4E-6% copper and 3E-4% boric acid
Color solution, containing 4E-4% iron, 4.5E-5% cobalt, 7E-6% manganese element yellow solution, contain 4E-4% biotin, 0.04%
P-aminobenzoic acid, 0.04% niacin, 0.035% thiamine hydrochloride colourless solution, blue solution: yellow solution: colourless
Solution=1:1:4.
S2, strain is added: maintains stirring, strain 39.54L is added by feeding port；
S3, selenium source is added: sodium selenite 416g is added after stirring 8~16h in step S2；
S4, deoxygenation operation: closing feeding port, is filled with CO2 into bucket by CO2 pipeline, oxygen in bucket is discharged；
S5, sealing are cultivated: cultivation temperature is set 26 DEG C by setting, opens lighting device, closed to cultivate 20 days；Cultivate 10
Stirring rate is adjusted to 50 revs/min after~15 days；
S6, discharge packing: opening discharge gate, will cultivate the organic selenium fertilizer completed and is discharged, encapsulating packing；
S7, cleaning-drying: by detergent line, clear water is added into cultivation bucket and is cleaned, it is dirty to open sewage draining exit discharge
Water repeats 3~4 times, opens bung and is dried, fixed bung after the completion of dry, in case next time uses.
Total selenium (in terms of Se) in organic selenium fertilizer obtained in the present embodiment is 202mg/ml, organic selenium 186mg/ml,
Conversion ratio reaches 92%.
Embodiment 3
The present embodiment from embodiment 1 there are different preparations to form.
A kind of method that the use of cultivation bucket prepares organic selenium fertilizer, comprising the following steps:
S1, culture medium is added: by inlet pipeline, distilled water 650L is added into cultivation bucket, feeding port is opened, to cultivation
Culture sill 3675g is added in bucket, agitating device is started by control box, stirring rate is set as 30 revs/min, will cultivate
Sill stirs evenly；Culture medium is prepared by the component of following mass percent: magnesium sulfate 184g, anhydrous calcium chloride 55g,
Anhydrous sodium acetate 1286g, urea 385g, potassium dihydrogen phosphate 535g, dipotassium hydrogen phosphate 790g, yeast extract 110g, microelement are molten
Liquid 330g, it is 4E-5% zinc, 4E-6% copper and 3E-4% boric acid that trace element solution, which includes containing mass percentage,
Blue solution, containing 4E-4% iron, 5E-5% cobalt, 7E-6% manganese element yellow solution, contain 4E-4% biotin, 0.04%
P-aminobenzoic acid, 0.04% niacin, 0.04% thiamine hydrochloride colourless solution, blue solution: yellow solution: colourless molten
Liquid=1:1:4.
S2, strain is added: maintains stirring, strain 39.85L is added by feeding port；
S3, selenium source is added: sodium selenite 485g is added after stirring 8~16h in step S2；
S4, deoxygenation operation: closing feeding port, is filled with CO2 into bucket by CO2 pipeline, oxygen in bucket is discharged；
S5, sealing are cultivated: cultivation temperature is set 26 DEG C by setting, opens lighting device, closed to cultivate 20 days；Cultivate 10
Stirring rate is adjusted to 50 revs/min after~15 days；
S6, discharge packing: opening discharge gate, will cultivate the organic selenium fertilizer completed and is discharged, encapsulating packing；
S7, cleaning-drying: by detergent line, clear water is added into cultivation bucket and is cleaned, it is dirty to open sewage draining exit discharge
Water repeats 3~4 times, opens bung and is dried, fixed bung after the completion of dry, in case next time uses.
Embodiment 4
The present embodiment from embodiment 1 there are different preparations to form.
A kind of method that the use of cultivation bucket prepares organic selenium fertilizer, comprising the following steps:
S1, culture medium is added: by inlet pipeline, distilled water 650L is added into cultivation bucket, feeding port is opened, to cultivation
Culture sill 3050g is added in bucket, agitating device is started by control box, stirring rate is set as 30 revs/min, will cultivate
Sill stirs evenly；Culture medium is prepared by the component of following mass percent: magnesium sulfate 140g, anhydrous calcium chloride 28g,
Anhydrous sodium acetate 1068g, urea 305g, potassium dihydrogen phosphate 427g, dipotassium hydrogen phosphate 640g, yeast extract 46g, trace element solution
396g, it is 3E-5% zinc that trace element solution, which includes containing mass percentage, the indigo plant of 3E-6% copper and 2E-4% boric acid
Color solution, containing 2E-4% iron, 2E-5% cobalt, 4E-6% manganese element yellow solution, contain 2E-4% biotin, 0.02% pair
Aminobenzoic acid, 0.02% niacin, 0.02% thiamine hydrochloride colourless solution, blue solution: yellow solution: colourless solution
=1:1:4.
S2, strain is added: maintains stirring, strain 39.5L is added by feeding port；
S3, selenium source is added: sodium selenite 416g is added after stirring 8~16h in step S2；
S4, deoxygenation operation: closing feeding port, is filled with CO2 into bucket by CO2 pipeline, oxygen in bucket is discharged；
S5, sealing are cultivated: cultivation temperature is set 26 DEG C by setting, opens lighting device, closed to cultivate 20 days；Cultivate 10
Stirring rate is adjusted to 50 revs/min after~15 days；
S6, discharge packing: opening discharge gate, will cultivate the organic selenium fertilizer completed and is discharged, encapsulating packing；
S7, cleaning-drying: by detergent line, clear water is added into cultivation bucket and is cleaned, it is dirty to open sewage draining exit discharge
Water repeats 3~4 times, opens bung and is dried, fixed bung after the completion of dry, in case next time uses.
Embodiment 5
The present embodiment from embodiment 1 there are different preparations to form.
A kind of method that the use of cultivation bucket prepares organic selenium fertilizer, is prepared: distillation by the component of following mass percent
Water 650L, culture medium 2775g, strain 39.7L, sodium selenite 485g；Culture medium by following mass percent component preparation and
At: magnesium sulfate 125g, anhydrous calcium chloride 25g, anhydrous sodium acetate 1055g, urea 278g, potassium dihydrogen phosphate 390g, phosphoric acid hydrogen two
Potassium 635g, yeast extract 42g, trace element solution 225g, it is 3.5E-5% that trace element solution, which includes containing mass percentage,
The blue solution of zinc, 3.5E-6% copper and 2.5E-4% boric acid contains 3E-4% iron, 4E-5% cobalt, 6E-6% manganese element
Yellow solution, contain 3E-4% biotin, 0.02% p-aminobenzoic acid, 0.03% niacin, 0.04% thiamine hydrochloride
Colourless solution, blue solution: yellow solution: colourless solution=1:1:4.
Embodiment 6
The present embodiment from embodiment 1 there are different preparations to form.
A kind of method that the use of cultivation bucket prepares organic selenium fertilizer, is prepared: distillation by the component of following mass percent
Water 650L, culture medium 2775g, strain 39.7L, sodium selenite 485g；Culture medium by following mass percent component preparation and
At: magnesium sulfate 125g, anhydrous calcium chloride 25g, anhydrous sodium acetate 1110g, urea 278g, potassium dihydrogen phosphate 390g, phosphoric acid hydrogen two
Potassium 583g, yeast extract 42g, trace element solution 222g, it is 3.5E-5% that trace element solution, which includes containing mass percentage,
The blue solution of zinc, 3.5E-6% copper and 2.5E-4% boric acid contains 3E-4% iron, 4E-5% cobalt, 6E-6% manganese element
Yellow solution, contain 3E-4% biotin, 0.02% p-aminobenzoic acid, 0.03% niacin, 0.04% thiamine hydrochloride
Colourless solution, blue solution: yellow solution: colourless solution=1:1:4.
Embodiment 7
The present embodiment from embodiment 1 there are different preparations to form.
A kind of method that the use of cultivation bucket prepares organic selenium fertilizer, is prepared: distillation by the component of following mass percent
Water 650L, culture medium 2775g, strain 39.7L, sodium selenite 485g；Culture medium by following mass percent component preparation and
At: magnesium sulfate 125g, anhydrous calcium chloride 25g, anhydrous sodium acetate 974g, urea 347g, potassium dihydrogen phosphate 458g, dipotassium hydrogen phosphate
582g, yeast extract 42g, trace element solution 222g, it is 3.5E-5% zinc that trace element solution, which includes containing mass percentage,
The blue solution of 3.5E-6% copper and 2.5E-4% boric acid, containing 3E-4% iron, 4E-5% cobalt, 6E-6% manganese element Huang
Color solution, the nothing containing 3E-4% biotin, 0.02% p-aminobenzoic acid, 0.03% niacin, 0.04% thiamine hydrochloride
Color solution, blue solution: yellow solution: colourless solution=1:1:4.
Embodiment 8
The present embodiment from embodiment 1 there are different preparations to form.
A kind of method that the use of cultivation bucket prepares organic selenium fertilizer, is prepared: distillation by the component of following mass percent
Water 650L, culture medium 2775g, strain 39.7L, sodium selenite 485g；Culture medium by following mass percent component preparation and
At: magnesium sulfate 125g, anhydrous calcium chloride 25g, anhydrous sodium acetate 1000g, urea 320g, potassium dihydrogen phosphate 430g, phosphoric acid hydrogen two
Potassium 611g, yeast extract 42g, trace element solution 222g, it is 3.5E-5% that trace element solution, which includes containing mass percentage,
The blue solution of zinc, 3.5E-6% copper and 2.5E-4% boric acid contains 3E-4% iron, 4E-5% cobalt, 6E-6% manganese element
Yellow solution, contain 3E-4% biotin, 0.02% p-aminobenzoic acid, 0.03% niacin, 0.04% thiamine hydrochloride
Colourless solution, blue solution: yellow solution: colourless solution=1:1:4.
Embodiment 9
The present embodiment from embodiment 1 there are different preparations to form.
A kind of method that the use of cultivation bucket prepares organic selenium fertilizer, is prepared: distillation by the component of following mass percent
Water 650L, culture medium 2775g, strain 39.7L, sodium selenite 485g；Magnesium sulfate 125g, anhydrous calcium chloride 25g, anhydrous sodium acetate
973g, urea 305g, potassium dihydrogen phosphate 388g, dipotassium hydrogen phosphate 695g, yeast extract 42g, trace element solution 222g, micro member
It is the blue molten of 3.5E-5% zinc, 3.5E-6% copper and 2.5E-4% boric acid that plain solution, which includes containing mass percentage,
Liquid, containing 3E-4% iron, 4E-5% cobalt, 6E-6% manganese element yellow solution, contain 3E-4% biotin, 0.02% pair of amino
Benzoic acid, 0.03% niacin, 0.04% thiamine hydrochloride colourless solution, blue solution: yellow solution: colourless solution=1:
1:4。
Described above to be merely exemplary for the purpose of the present invention, and not restrictive, those of ordinary skill in the art understand,
Without departing from the spirit and scope defined by the claims, any modification, the variation or equivalent that can be made, will all fall
Enter within protection scope of the present invention.
Claims (10)
1. a kind of cultivation bucket, including staving (1) and the bung (2) fixed with the staving (1) sealing, it is characterised in that: also wrap
The feeding port (3) being arranged above the staving (1) is included, the U-shaped outlet (4) being arranged below the staving (1) is arranged in institute
The transparent tube body (5) in the middle part of staving (1) is stated, the lighting device (51) internal in the transparent tube body (5) is set, is arranged described
The stirring in the transparent tube body (5) side is arranged in the hairbrush (6) contacted on the outside of transparent tube body (5) with the transparent tube body (5)
Device (12) is arranged on the outside of the staving (1) and is connected to the internal inlet pipeline (7) of the staving (1), detergent line
(8), CO2 pipeline (9), liquidometer (10), temperature sensor (11).
2. a kind of cultivation bucket according to claim 1, it is characterised in that: be additionally provided on the outside of the staving (1) for real
Now to the control box (13) of the cultivation bucket function control, for observing the observation window (14) for accompanying bacterium situation in bucket.
3. a kind of cultivation bucket according to claim 1, it is characterised in that: described U-shaped outlet (3) one end is sewage draining exit
(41), one end is discharge gate (42).
4. a kind of cultivation bucket according to claim 1, it is characterised in that: the hairbrush (6) and the agitating device (12)
It is fixedly connected, is rotated jointly by motor (15) drive being arranged on the bung (1).
5. a kind of cultivation bucket according to claim 1, it is characterised in that: the lighting device is along the transparent tube body
(4) 3 100W light bulbs of radially uniform setting.
6. a kind of cultivation bucket uses the method for preparing organic selenium fertilizer, which comprises the following steps:
S1, culture medium is added: by the inlet pipeline, distilled water is added in Xiang Suoshu cultivation bucket, opens the feeding port, to
The culture sill is added in the cultivation bucket, the agitating device is started by the control box, stirring rate is set as
30 revs/min, the culture sill is stirred evenly；
S2, strain is added: maintains stirring, strain is added by the feeding port；
S3, selenium source is added: sodium selenite is added after stirring 8~16h in step S2；
S4, deoxygenation operation: closing the feeding port, is filled with CO2 into bucket by the CO2 pipeline, oxygen in bucket is discharged；
S5, sealing are cultivated: cultivation temperature is set 26 DEG C by setting, opens lighting device, closed to cultivate 20 days；
S6, discharge packing: opening the discharge gate, will cultivate the organic selenium fertilizer completed and is discharged, encapsulating packing；
S7, cleaning-drying: clear water is added by the detergent line, in Xiang Suoshu cultivation bucket and is cleaned, the blowdown is opened
Mouth discharge sewage, repeats 3~4 times, opens the bung and be dried, and fixes the bung after the completion of dry, in case next time makes
With.
7. a kind of cultivation bucket according to claim 6 uses the method for preparing organic selenium fertilizer, it is characterised in that: in step S5
In, stirring rate is adjusted to 50 revs/min after cultivating 10~15 days.
8. a kind of cultivation bucket according to claim 6 uses the method for preparing organic selenium fertilizer, it is characterised in that: described organic
Selenium fertilizer is prepared by the component of following mass percent: distilled water 93.65%~93.8%, culture medium 0.4%~0.53%,
Strain 5.7%~5.8%, selenium source 0.06%~0.07%.
9. a kind of cultivation bucket according to claim 6 uses the method for preparing organic selenium fertilizer, it is characterised in that: the culture
Base is prepared by the component of following mass percent: magnesium sulfate 4.5%~6%, anhydrous calcium chloride 0.9%~2%, anhydrous second
Sour sodium 35%~40%, urea 10%~12.5%, potassium dihydrogen phosphate 14%~16.5%, dipotassium hydrogen phosphate 21%~25%,
Yeast extract 1.5%~3%, trace element solution 8%~13%.
10. a kind of cultivation bucket according to claim 9 uses the method for preparing organic selenium fertilizer, it is characterised in that: described micro-
It is 3E-5%~4E-5% zinc, 3E-6%~4E-6% copper and 2E-4% that secondary element solution, which includes containing mass percentage,
The blue solution of~3E-4% boric acid contains 2E-4%~4E-4% iron, 2E-5%~5E-5% cobalt, 4E-6%~7E-6% manganese
The yellow solution of element, containing 2E-4%~4E-4% biotin, 0.02%~0.04% p-aminobenzoic acid, 0.02%~
The colourless solution of 0.04% niacin, 0.02%~0.04% thiamine hydrochloride, each component volume ratio are blue solution: yellow is molten
Liquid: colourless solution=1:1:4.
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