KR101019133B1 - Plant nutrient substance for accelerating sugar content and coloration, and method of producing the same - Google Patents

Abstract

PURPOSE: A plant nutrient is provided to increase harvest by promoting sweetness, coloring, and increasing growth while avoiding damage to crops since harmful components such as organic acids are not included. CONSTITUTION: A plant nutrient for enhancing sugar content and coloration includes a fermentation extract of calcium superphosphate, rice bran, calcium supply source, and additives. The calcium supply source is selected from the group consisting of shrimp shell, crab shell, cuttlebone, octopus bone, small octopus bone, oyster shell, and their mixture. The additives are selected from the group consisting of citrus peel, Coptidis Rhizoma, Scutellariae Radix, lithospermi radix, plantago asiatica, Forsythiae Fructus, ulmus, Lonicerae Flos, leonuri Herba, Phaseoli radiati Semen, paeoniae Radix, and their mixture. The fermentation extract is fermented by inoculating combination strains of Lactobacillus sp., Issatchenkia sp., and Pichia sp.

Description

PLANT NUTRIENT SUBSTANCE FOR ACCELERATING SUGAR CONTENT AND COLORATION, AND METHOD OF PRODUCING THE SAME}

The present invention relates to a plant nutrient for sugar and color enhancement and a method for producing the same, and more particularly, to a plant nutrient for sugar and color enhancement and a method for producing the same, containing a fermented extract of superphosphate lime and rice bran.

“Fertilizer” is a generic term for substances that fertilize land and promote the growth of vegetation. According to the Fertilizer Control Act (Law 5019), “chemical changes in soil to nourish plants or help plant growth. Substance that brings about

In recent years, the demand for processed chemical preparations focused on the overuse of pesticides and chemical fertilizers for the rapid development of industry, the increase in the demand for high quality agricultural products, and the improvement of productivity, and the convenience for growing cultivation has been rapidly expanded. Self-control has been widely applied to soil.

Conventional farming methods concentrated on rich chemical fertilizers and pesticides accelerate salt accumulation in the soil, resulting in a decrease in the intellect and acidification of the soil, and the result of suppressing the final decomposer microorganisms and soil biosystems. It is becoming.

In addition to the increased use of pesticides to control pests that occur during plant growth, as well as increased resistance to pathogen control agents, the rapid increase and bundle of soil pathogens and harmful nematode densities due to the simplification and suppression of useful microorganisms in the soil. In turn, the vicious cycle of using a large amount of pesticides has been repeated.

In addition, severe salt accumulation due to repeated use of rich organic materials and fertilizers and antagonism between the nutrients resulting in a chain failure caused by the continuous lack of necessary nutrients and the lack of trace elements results in poor crop resistance. In addition, poor yields reduce crop yields and cause serious problems such as poor quality and taste.

In particular, a large amount of capital is being invested more than necessary for the forces of plants that are not recovering despite the continued use of expensive agricultural materials. From an economic point of view, the efficiency of such farming methods can be analyzed and based on an understanding of the level of economic damage allowance. There is a need.

Recently, with the problems of environmental pollution and the serious pollution of these crops, the development of farming methods using high quality organic fertilizers or composts rather than chemical fertilizers or pesticides has been spread, and many organic fertilizers have been developed and used. Efforts to improve the compost fermentation method have been continuously attempted.

However, despite these efforts, there is a lack of understanding and the lack of alternatives to the underlying causes of the decline in the intellect, and the intensive use of organic fertilizers and composts used as an alternative to chemical fertilizers is only materially different. In view of this, another problem similar to chemicals is being deepened.

Most compost raw materials are sawdust, various kinds of gourd, food waste, herbal sludge, fish meal or livestock meal and natural organic substances mixed with them.

In order to manufacture such organic fertilizers, fermentation is required for a long time, excessive equipment is required, and the water quality is contaminated with wastewater according to production.

If the sawdust is not sufficiently fermented, tannins and lignin may remain to damage the crops, and stocks and fish meal may cause organic acids to be damaged during the decay process and cause damage to crop roots.

In addition, the quality of organic raw materials used is not constant, and the quality and safety of agricultural materials cannot be guaranteed. Therefore, when the immature fertilizers containing excessive amounts of these organic materials are used for a long time, they are more likely to be used for the root obstacles due to salt concentration and the conversion to fungal soils. Caused by a multitude of fungal pathogens, lack of trace elements or lack of trace elements due to the antagonism between the fertilizer components to damage the growth of crops.

Since these natural fertilizers, compost, and chemical fertilizers contain few trace elements necessary for plant growth, they are insufficient to function as perfect plant growth nutrients, and too much organic matter is added to supply the nutrients needed by plants. The disadvantage is that it must be done.

In particular, since most of the organic materials used as the main raw materials have limitations in being imported, processed and distributed in foreign countries, they are related to intrinsic weakening of agricultural competitiveness.

In order to make up for the drawbacks of organic fertilizers, organic fertilizers have been proposed which include minerals containing large amounts of trace elements. For example, Korean Patent Application No. 91-4797 discloses a plant growth enhancing aid using mineral leadstone, and Korean Patent Application No. 84-8433 discloses organic soil for seedlings in which organic matter, zeolite, bentonite, etc. are mixed. Doing. Korean Patent Application No. 83-0633 discloses a mineral growth enhancer mainly composed of silica sand and elvan, and Korean Patent Application No. 2000-023644 manufactures biocapsules containing minerals and microorganisms containing a large amount of trace elements. Korean Patent Application No. 2001-6540 discloses a method for improving a complex soil containing germanium, and Patent 10-0368134 discloses a method for preparing a fertilizer containing minerals using filite, minerals and germanium. Doing.

However, these minerals appear to be meaningful in terms of their constituents, but may cause problems similar to those of chemical fertilizers in the drawback that crops are not readily available without sufficient degradation or solubilization by microorganisms.

On the other hand, phosphorus (phosphorus) is one of the essential elements for plant growth.

Phosphoric acid (phosphoric acid) is a component that plays an important role in the biochemical action, such as carbon assimilation, respiration, and the synthetic decomposition of starch or sugar, as well as make the tissue of the plant with components such as nucleic acids, proteins, phospholipids.

Phosphoric acid enhances eggplant and leaf growth, decreases the sourness of the fruit instead of increasing the sweetness of the fruit, increases the maturity of the fruit, and improves its shelf life, but when it is deficient (when the phosphorus content of the leaf is less than 0.07%) This becomes rough, coloration is delayed, and there is a lot of sourness.

Insufficient supply of phosphoric acid reduces not only the synthesis of nucleic acids but also a significant effect on protein synthesis. The most characteristic signs of phosphorus deficiency are the dark green leaves and the root system of plants. ) Development is limited, the stem is thinner, the growth is not developed, the height is shorter. Fruit trees (growth) is suppressed the growth of twigs and fine roots, and germination and development is sluggish. The formation of fruits and seeds is reduced, resulting in reduced yields and poor seed quality.

Due to the different production methods and sources of phosphate fertilizers, there are many kinds of phosphate fertilizers and their solubility and effectiveness.

The source of phosphate fertilizer is mainly phosphate slag. Phosphate is a kind of ore, formed by the accumulation of phosphorus of organisms in the past geological age. Can't exceed%

In addition, when the phosphate fertilizer is fertilized in the soil, the phosphate fertilizer is solubilized and changed to effective phosphoric acid after 90 days or more, the plant begins to absorb. In other words, in order for plants to absorb, they must be active phosphoric acid, aged in soil for at least 60 days and containing at least 3% organic matter.

However, the phosphoric acid fertilized in the soil has only about 15 to 30 days of residual efficacy, after which it is insoluble to turn into poorly soluble phosphoric acid, which is fixed and accumulated in the soil, and flows down when it rains to contaminate rivers and water. Let's go.

This large amount of fertilizer, which is almost entirely dependent on imports, is a problem in terms of national economy.

In addition, the phosphoric acid fertilizer is not only phosphoric acid is strong acid, but when the fertilizer is used as a fertilizer, the effectiveness of phosphoric acid is reduced by the poor solubility of the phosphoric acid adsorbed on the soil. The concentration of phosphoric acid has a low problem, and the accumulation of over-administered phosphate fertilizer into the soil has a problem of increasing the salt concentration in the soil, making the soil management difficult.

The present invention has been made to solve the above-mentioned problems of the prior art, and an object of the present invention is to provide a plant nutrient and a method for producing the same, which enhances plant growth, and improves sweetness and coloring.

The object of the present invention as described above is achieved by a plant nutrient for enhancing sugar and color, which contains fermented extracts of lime, rice bran and calcium sources.

The calcium source may be selected from the group consisting of shrimp shell, crab shell, insect shell, squid bone, octopus bone, octopus bone, oyster shell, clam shell, and mixtures thereof.

The fermented extract is obtained by fermentation further comprising a supplement selected from tangerine, rhubarb, golden, vinegar, plantain, poncho, rapeseed, gold and silver, motherwort, mung bean, peony and mixtures thereof.

The fermentation extract is fermented by inoculating a combination strain of lactic acid bacteria and yeast, the lactic acid bacteria are Lactobacillus (Lactobacillus), Streptococcus, (Leuconostoc), Pediococcus (Pediococcus), Lactococcus (Lactococcus) ), Bifidobacterium, and combinations thereof, and the yeast is Schizosaccharomyces, Lipomyces, Debaryomyces, Hanse Hansenula, Issatchenkia, Kluyveromyces, Pichia, Saccharomyces, Zygosaccharomyces, Candida, Cryptococcus It may be of the genus selected from the group consisting of (Cryptococcus), Kloeckera (Rloedotorula), Trigonopsis (Trigonopsis) and combinations thereof.

Preferably, the combination of strains of the lactic acid bacteria and yeast can be a combination of strains of Lactobacillus bacteria (Lactobacillus) spp, Escherichia director Chen (lssatchenkia) and Pichia spp (Pichia) sp.

It is still another object of the present invention to provide a method for preparing sugar and color enhancing plant nutrients containing fermented extracts of lime, rice bran and calcium sources, the method comprising: Is achieved by the manufacturing method:

a) fermenting a primary fermentation mixture comprising rice bran, calcium source and fermentation strain to prepare a primary fermentation product;

b) preparing a secondary fermentation product for fermenting and aging a secondary fermentation mixture comprising the primary fermentation product, superphosphate lime and fermentation strain; And

c) preparing a fermentation extract by extracting a liquid substance from the secondary fermentation product.

Preferably, in the method for producing a plant nutrient for enhancing sugar and color of the present invention,

After the step c) may comprise the step of aging the fermentation extract.

The calcium source may be selected from the group consisting of shrimp shell, crab shell, insect shell, squid bone, octopus bone, octopus bone, oyster shell, clam shell, and mixtures thereof.

The fermentation extract may be obtained by fermentation further comprising a supplement selected from tangerine, yellow lotus, golden, vinegar, plantain, poncho, rapeseed, gold and silver, motherwort, mung bean, peony and mixtures thereof.

The fermentation extract is fermented by inoculating a combination strain of lactic acid bacteria and yeast, the lactic acid bacteria are Lactobacillus (Lactobacillus), Streptococcus, (Leuconostoc), Pediococcus (Pediococcus), Lactococcus (Lactococcus) ), Bifidobacterium, and combinations thereof, and the yeast is Schizosaccharomyces, Lipomyces, Debaryomyces, Hanse Hansenula, Issatchenkia, Kluyveromyces, Pichia, Saccharomyces, Zygosaccharomyces, Candida, Cryptococcus It may be of the genus selected from the group consisting of (Cryptococcus), Kloeckera (Rloedotorula), Trigonopsis (Trigonopsis) and combinations thereof.

Fermentation extracts contained in the plant nutritional agent for enhancing sugar and coloring of the present invention do not contain harmful components such as organic acids that may damage crops as ingredients generated during fermentation extract preparation.

 Therefore, when using the plant nutrients of the present invention, the benefits such as sweetness and coloring enhancement effect can be obtained, but there is an advantage that no side effects damaging the crops occur.

In addition, the fermentation extract included in the plant nutritional agent for enhancing sugar and color of the present invention uses lime instead of chemical phosphoric acid in the preparation thereof, and such superphosphate contains many various and beneficial trace elements in addition to the phosphorus component. Therefore, there is an advantage that the beneficial trace elements are included in the phytonutrient of the present invention comprising such superphosphate fermented extract.

In addition, the fermentation extract included in the plant nutritional agent for enhancing sugar and color of the present invention can not only be sufficiently fermented in preparation thereof, but also by using reaction time, conditions, and microorganisms, which can be fermented into beneficial ingredients, such as superphosphate lime and the like. It is sufficiently decomposed and solubilized, thus, the plant nutrients of the present invention containing such fermented extracts can have a remarkable effect not only in terms of sugar, coloring, but also in terms of growth, enabling high-quality growth of plants and further increasing yield. In addition to shortening the harvest time, it can lead to higher prices.

In addition, according to the present invention it is possible to obtain an environmentally friendly sugar and color enhancing plant nutrients that do not contaminate rivers and land.

1 is a view showing citrus fruits according to a control group for comparison with the present invention in one embodiment of the present invention.
2 is a view showing a tangerine to which a plant nutrient for enhancing sugar and color is applied in one embodiment of the present invention.

Since the present invention relates to a plant nutritional agent for enhancing sugar and color and a method for producing the same, a plant nutritional agent for enhancing sugar and coloration will be described first, and then a manufacturing method thereof will be described.

The plant nutritional agent for enhancing sugar and color of the present invention contains fermented extracts of lime, rice bran and calcium sources.

The fermentation extract is obtained by fermenting a mixture of fermentation strains in lime, rice bran and calcium sources. More specifically, the fermentation extract is prepared by fermenting a primary fermentation mixture comprising a rice bran, calcium source and fermentation strain, to prepare a primary fermentation product, and a secondary fermentation mixture comprising such primary fermentation product, superphosphate lime and fermentation strains. After fermentation and ripening to prepare a secondary fermentation, it is a liquid material extracted from this secondary fermentation.

The rice bran refers to rice bran, except for rice bran and rice bran in general, there is a raw rice and unrefined rice bran remaining after processing without any processing, in the present invention can be used both fresh and degreasing rice, but preferred For example, raw rice bran having a particle size of 10 to 50 mesh is used.

The calcium source is selected from the group consisting of shrimp shells, crab shells, insect shells, squid bones, octopus bones, octopus bones, oyster shells, clam shells and mixtures thereof. Preferably, as a calcium source, a mixture of crab shell, oyster shell and clam shell is used, wherein the crab shell size is 1-5 mm, and the oyster shell and clam shell are in powder form having a size of about 100 mesh.

In addition, the fermented extract of the present invention further comprises a supplement selected from tangerine, rhubarb, golden, nachos, plantain, pontoon, rapeseed, sterling, motherwort, mung bean, peony and mixtures thereof in addition to superphosphate lime, rice bran and calcium sources. It may be obtained by fermentation. By adding such supplements, high quality sugars can be obtained and nutrition can be supplemented as a plant nutritional agent.

On the other hand, the fermentation strain is a combination strain of lactic acid bacteria and yeast, the lactic acid bacteria are Lactobacillus (Lactobacillus), Streptococcus, (Leuconostoc), Pediococcus (Pediococcus), Lactococcus (Lactococcus), BP The genus is selected from the group consisting of Gifidobacterium (Bifidobacterium) and combinations thereof, the yeast is Schizosaccharomyces, Lipomyces, Debaryomyces, Hansenula (Hansenula) ), Issatchenkia, Kluyveromyces, Pichia, Saccharomyces, Zygosaccharomyces, Candida, Cryptococcus , Kloeckera, Rhodotorula, Trigonopsis, and genus selected from the group consisting of a combination thereof.

Preferably, the fermentation strain is a combination strain of lactic acid bacteria and yeast, the lactic acid bacteria is selected from the group consisting of Lactobacillus confusa, and combinations thereof, the yeast is Isschenchen orientalis (Issatchenkia) orientalis), Pichia anomala, and combinations thereof.

More preferably, the fermentation strain is a combination strain of lactic acid bacteria and yeast, the lactic acid bacteria may be Lactobacillus confusa, the yeast may be a combination of Isachenchia orientalis and Pichia anomala.

The superphosphate lime is also referred to as fruit stone, and is an off-white or off-brown powder, and the composition is a mixture of calcium phosphate and gypsum (CaSO ₄ .2H ₂ O). Such superphosphate lime is prepared by reacting sulfuric acid with phosphate powder, and in general, the content of phosphoric acid in the superphosphate lime is 20% or more, and other gypsum occupies 60%. The superphosphate lime according to the present invention is preferably in the form of a powder, containing 20% or more of water-soluble phosphoric acid, and having a particle size of about 50 mesh.

In the fermented extract of the present invention as a result of measuring the active ingredient (according to the fertilizer quality test method), water soluble P ₂ 0 ₅ 3 ~ 10% by weight, CaO 0.6 ~ 5% by weight, K ₂ O 1 ~ 3% by weight, etc. Doing. This is a figure containing a lot of active ingredients calcium, potassium, phosphoric acid compared to the existing phosphate fertilizer.

In the plant nutrient for sugar and color enhancement of the present invention, the fermented extract of the present invention is preferably contained in an amount of 0.001 to 0.1% by weight based on the total weight of the plant nutrient. More preferably, it is used in an amount of 0.001 to 0.01% by weight, and most preferably in an amount of 0.002 to 0.003% by weight diluted fermentation extract by 300 to 500 times. When the content of the fermentation extract is less than 0.001 weight, the effect is insignificant problem, and when the content of the fermentation extract exceeds 0.1% by weight, the increase in the effect that can be obtained compared to the increase in content is insignificant. Therefore, the plant nutrient according to the present invention has the advantage that it has excellent efficacy even in a small amount.

The sugar and color enhancing plant nutrients of the present invention may further contain manure, urea, manure, lime nitrogen, ammonium sulfate, and the like containing nitrogen components in addition to the fermentation extract as described above, and ash and sulfuric acid containing potassium components. Potassium, potassium chloride, wood vinegar and the like may be further contained, and the phosphorus-containing ink and the like may be further added to enhance the content of nitrogen, potassium and phosphorus, which are three major elements necessary for plant growth.

A process for preparing sugar and color enhancing plant nutrients containing fermented extracts of the superphosphate, rice bran and calcium sources of the present invention comprises the following steps:

a) fermenting a primary fermentation mixture comprising rice bran, calcium source and fermentation strain to prepare a primary fermentation product;

b) preparing a secondary fermentation product for fermenting and aging a secondary fermentation mixture comprising the primary fermentation product, superphosphate lime and fermentation strain; And

c) preparing a fermentation extract by extracting a liquid substance from the secondary fermentation product.

The method for producing a plant nutrient for enhancing sugar and color of the present invention may include the step of aging the fermentation extract after step c).

In step a), the primary fermentation mixture comprising rice bran, calcium source and fermentation strain is fermented to prepare a primary fermentation product.

The rice bran refers to rice bran over the rice and excluding rice husk as described above, there is generally left untreated rice and rice bran oil left untreated, in the present invention can be used both fresh and degreasing rice It is possible to use, but preferably using a rice bran having a particle size of 10 ~ 50mesh. Rice bran is used as a food for lactic acid bacteria microorganisms, yeast, and the like.

The calcium source is selected from the group consisting of shrimp shells, crab shells, insect shells, squid bones, octopus bones, octopus bones, oyster shells, clam shells and mixtures thereof.

Preferably, as a calcium source, a mixture of crab shell, oyster shell and clam shell is used, wherein the size of the crab shell is 1-5 mm in particle size, and the oyster shell and clam shell have a particle size of about 100 mesh. to be.

The calcium source is used in an amount of 70 to 110 parts by weight, preferably 80 to 100 parts by weight, based on 100 parts by weight of the rice bran.

In addition, the fermentation extract of the present invention can be used to obtain a fermentation further comprising a supplement selected from tangerine, yellow lotus, gold, nachos, plantain, pontoon, rapeseed bark, gold silver, motherwort, mung bean, peony and mixtures thereof. By adding such supplements, high quality sugars can be obtained and nutrition can be supplemented as a plant nutritional agent.

The supplements described above may be mixed in the primary fermentation step, or may be mixed in the secondary fermentation step or the liquid phase extraction from the secondary fermentation step. However, it is better to mix in the first fermentation stage. Preferably such a supplement is used in an amount of 0.01 to 5 parts by weight, preferably 0.1 to 3 parts by weight, based on 100 parts by weight of the rice bran, while reducing the cost burden with a small amount of addition, further maximizing the efficacy of the plant nutrients. It is effective.

The fermentation strain is a combination strain of lactic acid bacteria and yeast, the lactic acid bacteria are Lactobacillus (Lactobacillus), Streptococcus, (Leuconostoc), Pediococcus (Pediococcus), Lactococcus (Lactococcus), Bifidobacte Lilium (Bifidobacterium) is a genus selected from the group consisting of, and the yeast is Schizosaccharomyces, Lipomyces (Depomyomys), Debaryomyces (Hansenula), Issatchenkia, Kluyveromyces, Pichia, Saccharomyces, Zygosaccharomyces, Candida, Cryptococcus, Cryptococcus It may be of the genus selected from the group consisting of Kloeckera, Rhodotorula, Trigonopsis and combinations thereof.

Preferably, the fermentation strain is a combination strain of lactic acid bacteria and yeast, the lactic acid bacteria is selected from the group consisting of Lactobacillus confusa, and combinations thereof, the yeast is Isschenchen orientalis (Issatchenkia) orientalis), Pichia anomala, and combinations thereof.

More preferably, the fermentation strain is a combination strain of lactic acid bacteria and yeast, the lactic acid bacteria may be Lactobacillus confusa, the yeast may be a combination of Isachenchia orientalis and Pichia anomala.

The fermentation strain is used in the form of a fermentation strain solution containing a fermentation strain of 1 × 10 ⁵ cfu / ml to 5 × 10 ⁵ cfu / ml in the liquid state, the fermentation strain solution is 5 to 100 parts by weight of the rice bran To 20 parts by weight, preferably 7 to 15 parts by weight.

Mix the fermentation strain solution 0.75-6kg and lactose 10-100g containing fermentation strain of 1 × 10 ⁵ cfu / ml to 5 × 10 ⁵ cfu / ml in calcium source 10.5-33kg, liquid state to 15-30 kg of the above-mentioned rice bran To prepare a primary fermentation mixture and then put it under a temperature condition of 30 to 37 ℃ fermentation for 96 to 168 hours to prepare a primary fermentation product.

At this time, the fermentation conditions vary depending on the characteristics of each strain, but may be made in aerobic or anaerobic conditions, but preferably made in aerobic anaerobic conditions. In addition, a carbon source and / or pH adjuster may be further added to further activate the culture of the fermentation bacteria. It is preferable that pH at fermentation is 3-4.

This fermentation process decomposes rice bran and calcium sources, and also generates lactic acid, which promotes the reproductive growth of crops by forming fermented products of well-mixed phosphoric acid of later added phosphate lime.

In step b), a secondary fermentation product is prepared to ferment the secondary fermentation mixture comprising the primary fermentation product, the superphosphate lime and the fermentation strain obtained in step a). Such superphosphate lime is used in an amount of 250 to 750 parts by weight based on 100 parts by weight of the primary fermentation product.

As described above, the superphosphate lime is also referred to as permeate, which is an off-white or off-brown powder, and the composition is a mixture of calcium phosphate and gypsum (CaSO ₄ .2H ₂ O). Such superphosphate lime is prepared by reacting sulfuric acid with phosphate powder, and in general, the content of phosphoric acid in the superphosphate lime is 20% or more, and other gypsum occupies 60%.

The superphosphate lime used in step b) is preferably used in a powder size of less than 100 mesh, the powder of superphosphate lime is smaller than 100 mesh in order to be well fermented and mixed well with rice bran and calcium source Use it in size.

The fermentation strain is a combination strain of lactic acid bacteria and yeast, the lactic acid bacteria are Lactobacillus (Lactobacillus), Streptococcus, (Leuconostoc), Pediococcus (Pediococcus), Lactococcus (Lactococcus), Bifidobacte Lilium (Bifidobacterium) is a genus selected from the group consisting of, and the yeast is Schizosaccharomyces, Lipomyces (Depomyomys), Debaryomyces (Hansenula), Issatchenkia, Kluyveromyces, Pichia, Saccharomyces, Zygosaccharomyces, Candida, Cryptococcus, Cryptococcus It may be of the genus selected from the group consisting of Kloeckera, Rhodotorula, Trigonopsis and combinations thereof.

Preferably, the fermentation strain is a combination strain of lactic acid bacteria and yeast, the lactic acid bacteria is selected from the group consisting of Lactobacillus confusa, and combinations thereof, the yeast is Isschenchen orientalis (Issatchenkia) orientalis), Pichia anomala, and combinations thereof.

More preferably, the fermentation strain is a combination strain of lactic acid bacteria and yeast, the lactic acid bacteria may be Lactobacillus confusa, the yeast may be a combination of Isachenchia orientalis and Pichia anomala.

Fermented strain is used in the form of a fermentation strain solution containing a fermentation strain of 1 × 10 ⁵ cfu / ml to 5 × 10 ⁵ cfu / ml in the liquid state, the fermentation strain solution is based on 100 parts by weight of the primary fermentation It is used in an amount of 15 to 35 parts by weight.

That is, in step b), 50 kg to 150 kg of the above-mentioned superphosphate lime is mixed with 20 kg of the primary fermentation product prepared in step a), and water (which includes not only general water but distilled water, ground water, marine water or deep water) is 5 ~ A secondary fermentation mixture was prepared by further mixing 3 to 7 kg of fermentation strain solution containing fermentation strains of 15 kg, lactose 1-10 kg and 1 × 10 ⁶ cfu / ml to 5 × 10 ⁶ cfu / ml cfu / ml in liquid form. Then, it was put under a temperature condition of 30 to 37 ℃ and stirred for 5 to 40 minutes at 50 to 80 rmp once a week to 5 times a week to ferment and mature for 3 to 5 weeks to prepare a secondary fermentation product do.

At this time, the fermentation conditions vary depending on the characteristics of each strain, but may be made in aerobic or anaerobic conditions, but preferably made in aerobic anaerobic conditions. In addition, a carbon source and / or pH adjuster may be further added to further activate the culture of the fermentation bacteria. It is preferable that pH at fermentation is 3-4.

This fermentation process not only further decomposes rice bran and calcium sources, but also decomposes the superphosphate lime so that lactic acid is generated and fermentation is promoted, thereby promoting the reproductive growth of crops.

In the step c), extract the liquid substance from the secondary fermentation to prepare a fermentation extract.

The secondary fermentation obtained from step b) is dark brown, and in particular, the size is smaller than 50 mesh due to decomposition of rice bran or superphosphate lime contained in the secondary fermentation.

300-500 kg of water is mixed with 200 kg of this secondary fermentation, which is stirred for 10 to 40 minutes at a speed of 1,500 to 2,000 rpm. After leaving for about 48 hours, the supernatant is obtained to obtain the fermented extract of the present invention.

The fermented extract, which is the supernatant, may be subjected to further aging step, and the aging method is as follows.

The fermented strain solution 500g ~ 2kg and lactose 50g to 200g containing a fermentation strain of 1 × 10 ⁶ cfu / ml to 5 × 10 ⁶ cfu / ml in a liquid state to 150 kg of the supernatant obtained above is mixed, 27-35 ℃ It is aged for 25 to 40 days with stirring at a rate of 50 to 60 rpm at a temperature of 10 to 30 times a day, once for about 5 to 20 minutes at a temperature.

The fermentation strain is as described above.

Fermented extracts of the invention may be further processed or formulated.

For reference, after fermenting rice bran in the present invention, the mixture is fermented by mixing rice bran and superphosphate lime, and undergoes a third fermentation process by extracting a liquid substance thereof, whereby, the first fermentation by mixing rice bran and superphosphate lime There is an advantage that it is possible to increase the success rate of fermentation rather than.

The plant nutrient for enhancing sugar and color of the present invention preferably contains the fermented extract of the present invention prepared by the method as described above in an amount of 0.001 to 0.1% by weight based on the total weight of the plant nutrient. More preferably, it is used in an amount of 0.001 to 0.01% by weight, and most preferably in an amount of 0.002 to 0.003% by weight diluted fermentation extract by 300 to 500 times. When the content of the fermentation extract is less than 0.001 weight, the effect is insignificant problem, and when the content of the fermentation extract exceeds 0.1% by weight, the increase in the effect that can be obtained compared to the increase in content is insignificant.

In addition, the sugar and color enhancing plant nutrient of the present invention may further contain manure, urea, manure, lime nitrogen and ammonium sulfate, etc., containing nitrogen, in addition to the fermentation extract as described above, and ash containing potassium It may further contain potassium sulfate, potassium chloride, wood vinegar, and the like, and may further contain a phosphorus-containing ink and the like, thereby reinforcing the content of nitrogen, potassium, and phosphorus, three major elements required for plant growth.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples.

( Example  1: preparation of plant nutrient 1 according to the present invention)

20 kg of raw rice bran powdered to 30 mesh in size, 400g of crab shell, 400g of oyster shell and 400g of clam shell were fermented strains of 1.5 × 10 ⁶ cfu / ml in liquid form (Lactobacillus confusa, Isachen). Combination strains of Kia Orientalis and Pichia anomala, the ratio of 1: 1 to 1) and 2kg of fermentation strain solution containing lactose 50g were mixed to prepare a primary fermentation mixture and placed under a temperature condition of 35 ℃ Fermentation was performed for 100 hours to prepare a primary fermentation product. At this time, the fermentation conditions are aerobic anaerobic conditions, the pH was maintained at 3 to 4 by adding a pH adjuster.

20 kg of the primary fermented product, 100 kg of superphosphate lime in powder form smaller than 100 mesh are mixed and fermented strain of 1.5 × 10 ⁶ cfu / ml in uncontaminated groundwater 10 kg, lactose 5 kg and liquid state. 5 kg of fermentation strain solution containing (combined strain of Lactobacillus confusa, Isachenchia orientalis and Pichia anomala, the ratio is 1: 1), further mixed to prepare a secondary fermentation mixture A secondary fermentation product was prepared by fermenting and aging for 4 weeks while stirring at 36 rmp for 30 minutes at a temperature of 35 ° C. once a week. At this time, the fermentation conditions are also anaerobic conditions, the pH was adjusted to 3 to 4 by adding a pH regulator.

400 kg of water was mixed with 200 kg of the secondary fermented product, which was stirred for 20 minutes at a speed of 1,800 rpm, and left for about 48 hours to obtain 150 kg of the supernatant. Fermentation strain of 1.5 × 10 ⁶ cfu / ml (Lactobacillus confusa, Ischenchenia orientalis and Pichia anomala as a combination strain of 1: 1 × 1) in a liquid state in 150 kg of the supernatant. 1 kg of fermentation strain solution and 100 g of lactose were mixed and aged for 30 days with stirring at a rate of 50 to 60 rpm once a day for 24 minutes at a temperature of 30 ° C. for 30 days to obtain a fermentation extract of the present invention.

The fermented extract obtained above was diluted 500-fold to prepare a plant nutrient for enhancing sweetness and coloration, containing 0.002% by weight.

( Example  2: preparation of plant nutritional agent 2 according to the present invention)

Fermented strain of 1.5 × 10 ⁶ cfu / ml (Lactobacillus confusa, 400g of crab shell, 400g of oyster shell, 400g of clam shell, and 300g of tangerine) as a calcium source in 20 kg of fresh rice bran powdered to a size of 30mesh. A primary strain was prepared by mixing 2 kg of fermentation strain solution containing Ischachenia orientalis and Pichia anomala, with a ratio of 1: 1: 1) and lactose 50 g, to prepare a primary fermentation mixture. Place under fermentation for 100 hours to prepare a primary fermentation. At this time, the fermentation conditions are aerobic anaerobic conditions, and the pH was maintained at 3 to 4 by adding a pH adjuster.

20 kg of the primary fermented product, 100 kg of superphosphate lime in powder form smaller than 100 mesh are mixed and fermented strain of 1.5 × 10 ⁶ cfu / ml in uncontaminated groundwater 10 kg, lactose 5 kg and liquid state. 5 kg of fermentation strain solution containing (combined strain of Lactobacillus confusa, Isachenchia orientalis and Pichia anomala, the ratio is 1: 1), further mixed to prepare a secondary fermentation mixture A secondary fermentation product was prepared by fermenting and aging for 4 weeks while stirring at 36 rmp for 30 minutes at a temperature of 35 ° C. once a week. At this time, the fermentation conditions are also anaerobic conditions, the pH was adjusted to 3 to 4 by adding a pH regulator.

400 kg of water was mixed with 200 kg of the secondary fermented product, which was stirred for 30 minutes at a speed of 1,650 rpm, and left for about 48 hours to obtain 150 kg of the supernatant. Fermentation strain of 1.5 × 10 ⁶ cfu / ml (Lactobacillus confusa, Ischenchenia orientalis and Pichia anomala as a combination strain of 1: 1 × 1) in a liquid state in 150 kg of the supernatant. 1 kg of fermentation strain solution and 100 g of lactose were mixed and aged for 30 days with stirring at a rate of 50 to 60 rpm once a day for 24 minutes at a temperature of 30 ° C. for 30 days to obtain a fermentation extract of the present invention.

The fermented extract obtained above was diluted 500-fold to prepare a plant nutrient for enhancing sugar and color, containing 0.002% by weight.

( Experimental Example  1: component analysis experiment)

Component analysis of the plant nutritional agent 1 of the present invention according to Example 1 was commissioned to Cheil Analysis Center (Analysis Sentime located in Seonggok-dong, Danwon-gu, Ansan-si, Gyeonggi-do), and the results are shown in Table 1 below. In addition, Table 2 shows the results of component analysis of the plant nutritional agent 2 of the present invention according to Example 2.

May 10, 2010 Analysis Results Cheil Analysis Co., Ltd. Item result Remarks Water soluble CaO (%) 0.74 Analytical Method: Fertilizer Quality Inspection
PH and electrical conductivity measured at a ratio of 1: 500 (extract: distilled water) Water soluble P2O5 (%) 4.58 Water Soluble K2O (%) 1.64

May 10, 2010 Analysis Results Cheil Analysis Co., Ltd. Item result Remarks Water soluble CaO (%) 0.77 Analytical Method: Fertilizer Quality Inspection
PH and electrical conductivity measured at a ratio of 1: 500 (extract: distilled water) Water soluble P2O5 (%) 5.19 Water Soluble K2O (%) 1.83

According to Table 1 and Table 2 above, when a large amount of P component, K component and Ca component are contained and the plant is foliar sprayed and fertilized directly on the root, reproductive growth is enhanced, and the color of sugar, foliar, etc. is remarkably increased. Appears to improve.

( Experimental Example  2: Sweetness  And color degree comparison experiments)

In order to confirm the effect of the plant nutrients according to Examples 1 and 2 on the sweetness of fruit, the plant nutrients treatment group of the present invention (Example 1) and the treatment liquid of Comparative Example ( The ferment distributed by Co., Ltd. was used as 500-fold irrigation) and the untreated group (control group) were evaluated in three groups. The plant nutrients (Example 1) of the present invention and the treatment solution of Comparative Example were each treated three times at weekly intervals while being applied to citrus trees using a sprayer. After 3 weeks, the sugar content was measured using a sugar group of PR-201 (ATAGI CO. Ltd. Japan). As a result of the measurement of sugar content, the control group recorded 9.0 ° Brix, and the comparison group was found to have a slightly higher sugar content than the control group as 9.5 ° Brix. On the other hand, the plant nutritional treatment group according to the present invention was 11.2 ° Brix showed a significant synergistic effect compared to the control and the comparative example.

In addition, the appearance of citrus according to the plant nutrient treatment group and the control group according to the present invention is shown in Figures 1 and 2, Figure 1 is treated with a control, Figure 2 is treated with a plant nutrient according to the present invention. Although the same time has elapsed, it can be seen that the citrus tree according to the present invention is more vivid and thicker, from which the plant nutrient according to the present invention promotes coloration and promotes growth.

The plant nutrient for improving sugar and coloring according to the present invention can be seen that a significant contribution to the improvement of sweetness, coloring and growth despite the use of a small amount (sample amount: distilled water = 1: 500).

Claims (8)

As a phytonutrient for sugar and color enhancement, containing fermented extract of superphosphate lime, rice bran, calcium source and supplement,
The calcium source is selected from the group consisting of shrimp shell, crab shell, insect shell, squid bone, octopus bone, octopus bone, oyster shell, clam shell and mixtures thereof,
The supplement is selected from tangerine, barberry, golden, vinegar, plantain, poncho, rapeseed, gold and silver, motherwort, mung bean, peony, and mixtures thereof,
The fermented extract is fermented by inoculating a combination strain of Lactobacillus sp. Strain, Ischiachenia strain and Pichia spp. Strain,
Phytonutrients for enhancing sugar and coloring. According to claim 1, wherein the fermented extract is contained in an amount of 0.001 to 0.1% by weight based on the total weight of the plant nutrients, plant nutrients for enhancing sugar and color. As a method for producing sugar and coloring enhancement plant nutrients containing fermented extract of superphosphate lime, rice bran, calcium source and supplement,
a) fermenting a primary fermentation mixture comprising rice bran, calcium source and fermentation strain to prepare a primary fermentation product;
b) preparing a secondary fermentation product for fermenting and aging a secondary fermentation mixture comprising the primary fermentation product, superphosphate lime and fermentation strain; And
c) preparing a fermentation extract by extracting a liquid substance from the secondary fermentation product.
Including,
Fermentation was prepared by adding a supplement to the primary fermentation mixture of step a) to produce a primary fermentation, or fermentation and ripening by adding further supplements to the secondary fermentation mixture of step b) to prepare a secondary fermentation product. Alternatively, or further mixing the supplement to the secondary fermentation of step c) and extracting a liquid substance therefrom to prepare a fermentation extract,
The calcium source is selected from the group consisting of shrimp shell, crab shell, insect shell, squid bone, octopus bone, octopus bone, oyster shell, clam shell and mixtures thereof,
The supplement is selected from tangerine, rhubarb, gold, vinegar, plantain, poncho, rapeseed, gold and silver, motherwort, mung bean, peony, and mixtures thereof,
The fermentation strain is a combination strain of the genus Lactobacillus strain, Ischenchenia strain and Pichia genus strain,
Method for producing a plant nutritional agent for enhancing sweetness and coloration. The method of claim 3, comprising the step of aging the fermentation extract after step c), the method of producing a plant nutrition for enhancing sugar and color. delete delete delete delete

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