CN100564512C - A kind of photosynthetic bacterium enriched substratum - Google Patents
A kind of photosynthetic bacterium enriched substratum Download PDFInfo
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- CN100564512C CN100564512C CNB2007100119970A CN200710011997A CN100564512C CN 100564512 C CN100564512 C CN 100564512C CN B2007100119970 A CNB2007100119970 A CN B2007100119970A CN 200710011997 A CN200710011997 A CN 200710011997A CN 100564512 C CN100564512 C CN 100564512C
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- photosynthetic bacterium
- substratum
- basic medium
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- yeast extract
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Abstract
The present invention relates to a kind of photosynthetic bacterium enriched substratum.The technical scheme that adopts is: a kind of photosynthetic bacterium enriched substratum is characterized in that containing basic medium, yeast extract paste and hydroxy-butanedioic acid; In basic medium, every 1000ml basic medium adds the yeast extract paste of 0.1g～3.0g and the hydroxy-butanedioic acid of 0.5g～2.0g.With culture medium culturing photosynthetic bacterium of the present invention, cultivation speed is fast, and cost is low.Remedied the raise weak point of aspect such as too fast of too short and reproductive process pH of photosynthetic bacterium growth phase, prolonged 5～7 days vegetative period, made maximum growth amount OD
660Bring up to 1.6～3.0.
Description
Technical field:
The present invention relates to a kind of substratum of enrichment of bacterial, particularly a kind of photosynthetic bacterium enriched substratum.
Background technology:
Photosynthetic bacterium is that a big class can be carried out photosynthetic procaryotic general name.The practice that research is used shows, photosynthetic bacterium can utilize multiple sulfide and organism as its photosynthetic hydrogen donor and organic carbon source, at the regulating and controlling water quality of the treatment of Organic Wastewater of high density and resource utilization, aquaculture and promote healthy growth, in agricultural as aspects such as high-efficiency activated bacterial manure, bringing into play very useful and effect that attract people's attention.
Photosynthetic bacterium is the general name of prokaryotic micro-organisms of the special physiological monoid of a class non-oxygen-production, is the prokaryotic micro-organisms with primary light synthetic system that occurs the earliest, plays an important role in natural carbon element, nitrogen, thionin conversion cycles.Photosynthetic bacterium is nutritious, and protein content is up to more than 60% in the cell dry-matter, and photosynthetic bacterium also contains multivitamin, and especially vitamin B group is very abundant, and the content of VB12, folic acid, pantothenic acid, vitamin H is far above yeast.In addition, photosynthetic bacterium also contains physiologically active substances such as a large amount of carotenoid, ubiquinone.Therefore, photosynthetic bacterium has very high nutritive value, and very high using value is arranged on aquatic products and livestock and poultry cultivation.
The promotion and application of photosynthetic bacterium, key need to be suitable medium matter, but at present classical be used for photosynthetic bacterium enriched conventional substratum, main prescription is: ammonium chloride 1g, sodium bicarbonate 1g, dipotassium hydrogen phosphate 0.2g, sodium acetate 1～5g, magnesium sulfate heptahydrate 0.2g, sodium-chlor 0.5～2g, somatomedin 10ml, trace element solution 10ml, distilled water 980ml, (seeing " microbiology experiment study course "), the time of its average enrichment was 2～8 weeks, the cycle is long, and exist the medium component kind many, the shortcoming that cost is higher.
Summary of the invention:
In order to address the above problem, the invention provides a kind of photosynthetic bacterium enriched substratum, with culture medium culturing photosynthetic bacterium of the present invention, cultivation speed is fast, and cost is low.
The technical solution used in the present invention is: a kind of photosynthetic bacterium enriched substratum is characterized in that containing basic medium, yeast extract paste and hydroxy-butanedioic acid (oxysuccinic acid); In basic medium, every 1000ml basic medium adds the yeast extract paste of 0.1g～3.0g and the hydroxy-butanedioic acid of 0.5g～2.0g.
Preferably, in basic medium, every 1000ml basic medium adds the yeast extract paste of 1.0g and the hydroxy-butanedioic acid of 1.5g.
Basic medium is the substratum commonly used of photosynthetic bacterium.What just warp was used always in the prior art, and disclosed photosynthetic bacteria culture medium.
Regulating medium pH of the present invention is 7～9,121 ℃ of sterilization 30min.Photosynthetic bacterium is inoculated in the substratum, and the vibration mixing places the illumination box illumination cultivation, and illumination condition is 1500～2000lux, and temperature is 30 ± 1 ℃.
Yeast extract paste increases along with the increase of the concentration of yeast extract paste the influence of the increment of photosynthetic bacterium, and after 7 days illumination cultivation, when the consumption of yeast extract paste during less than 3g/L (every 1000ml basic medium adds 3.0g), its thalline reproduction speed is very fast, bacterium liquid OD
660Reach 1.9, when the consumption of yeast extract paste during greater than 3g/L, its OD
660Be worth no considerable change, bacterium liquid OD
660Be 2.0～2.1.Therefore, the consumption of yeast extract paste is advisable with the yeast extract paste of 0.1g/L～3.0g/L.
Hydroxy-butanedioic acid is to the influence of the increment of photosynthetic bacterium, increase along with the increase of the concentration of hydroxy-butanedioic acid, after 7 days illumination cultivation, when the consumption of hydroxy-butanedioic acid less than 2.0g/L when (every 1000ml basic medium adds 2.0g), its thalline reproduction speed is very fast, bacterium liquid OD
660Reach 1.7, when the consumption of hydroxy-butanedioic acid during greater than 2.0g/L, its OD
660Be worth no considerable change, bacterium liquid OD
660Be 1.8～1.9.Therefore, the consumption of hydroxy-butanedioic acid is advisable with 0.5g/L～2.0g/L.
The invention has the beneficial effects as follows:
1, enrichment culture speed is fast.Owing in basic medium, added nutritive ingredients such as yeast extract paste, hydroxy-butanedioic acid,, made photosynthetic bacterium growth speed fast for photosynthetic bacterium has been created a better anaerobic environment, cultivate after 5 days, bacterium liquid color deepens gradually, pinkly becomes dark redly at last by pale red changing into, cultivates its OD
660Be 1.7～2.0.
2, the photosynthetic bacterium growth phase prolongs, and the maximum growth amount improves.Along with the increase of incubation time, bacterium liquid OD
660Increase gradually, when cultivating 4 days, its OD
660Be 1.2～1.5, the OD during than use base culture base photosynthetic bacterium
660Increase, when cultivating 8～12 days, bacterium liquid increment reaches the climax, its OD
660Be 2.0～3.0.Prolonged 3～7 days the vegetative period of photosynthetic bacterium, and its breeding is more had promoter action.
3, use culture medium culturing photosynthetic bacterium of the present invention, the more conventional basic medium of pH value rising is slow.In the photosynthetic bacterium growth process, along with the constantly rising of growth pH value of photosynthetic bacterium, when the pH value rose to 9 in the basic medium, bacterial growth was suppressed.And substratum of the present invention rises slowly with respect to basic medium pH value, when the pH value rises to 9, and the OD of photosynthetic bacterium
660Value begins to descend.Mainly be because the pH value of substratum has influenced the degree of ionization of nutritive substance in the substratum, thereby influence the absorption of microorganism, but also can influence the activity of various enzymes in the metabolic reaction nutritive substance.
4, cost is low.In conventional substratum, owing to added somatomedin and trace element solution, use cost is too high, and the present invention has substituted somatomedin and trace element solution with yeast extract paste and hydroxy-butanedioic acid, and cost is reduced.
5,, be used for purifying and improving aquaculture water with the photosynthetic bacterium of culture medium culturing of the present invention.30kg/hm by photosynthetic bacterium
2The disposable fish pond that is spilled into of consumption, test tank water-quality ratio control group has clear improvement, and wherein dissolved oxygen increases by 68%, and COD descends 21%, and ammonia nitrogen descends 58.7%, and nitrate nitrogen descends 29.4%, and sulfide descends 77.4%.
Description of drawings:
Fig. 1 is the present invention and conventional substratum, in substratum enrichment culture process, and optical density(OD) OD
660Variation diagram;
Fig. 2 is the present invention and conventional substratum, in substratum enrichment culture process, and the pH variation diagram.
In Fig. 1 and Fig. 2, a represents substratum of the present invention, and b represents conventional substratum
Embodiment:
(1) photosynthetic bacterium enriched culture medium preparation
Basic medium: ammonium chloride 1.0g, sodium bicarbonate 1.0g, dipotassium hydrogen phosphate 0.2g, sodium acetate 3.0g, magnesium sulfate heptahydrate 0.2g, sodium-chlor 1.0g is made into 1000ml with distilled water.
In above-mentioned basic medium, add 0.1g yeast extract paste and 0.5g hydroxy-butanedioic acid.
(2) cultivation of photosynthetic bacterium
The pH of above-mentioned substratum is transferred to 7,121 ℃ of sterilization 30min.Is 4% to be inoculated in the substratum photosynthetic bacterium (commercial) according to inoculum density, and the vibration mixing places the illumination box illumination cultivation, and illumination condition is 1500lux, 30 ℃ of temperature.
Enrichment culture result: illumination cultivation is after 5 days, bacterium liquid OD
660Be 1.3, illumination cultivation reached the maximum growth amount after 8 days, bacterium liquid OD
660Be 1.9, make the logarithmic phase of photosynthetic bacterium prolong 3 days.
(1) photosynthetic bacterium enriched culture medium preparation
Basic medium: ammonium chloride 1.0g, sodium bicarbonate 1.0g, dipotassium hydrogen phosphate 0.2g, sodium acetate 3.0g, magnesium sulfate heptahydrate 0.2g, sodium-chlor 1.0g is made into 1000ml with distilled water.
In above-mentioned basic medium, add 3.0g yeast extract paste and 2.0g hydroxy-butanedioic acid.
(2) cultivation of photosynthetic bacterium
The pH of above-mentioned substratum is transferred to 7,121 ℃ of sterilization 30min.Is 4% to be inoculated in the substratum photosynthetic bacterium (commercial) according to inoculum density, and the vibration mixing places the illumination box illumination cultivation, and illumination condition is 1500lux, 30 ℃ of temperature.
Enrichment culture result: illumination cultivation is after 5 days, bacterium liquid OD
660Be 1.4, illumination cultivation reached the maximum growth amount after 10 days, bacterium liquid OD
660Be 2.2, make the logarithmic phase of photosynthetic bacterium prolong 5 days.
(1) photosynthetic bacterium enriched culture medium preparation
Basic medium: ammonium chloride 1.0g, sodium bicarbonate 1.0g, dipotassium hydrogen phosphate 0.2g, sodium acetate 3.0g, magnesium sulfate heptahydrate 0.2g, sodium-chlor 1.0g is made into 1000ml with distilled water.
In above-mentioned basic medium, add 1.0g yeast extract paste and 1.5g hydroxy-butanedioic acid.
(2) cultivation of photosynthetic bacterium
The pH of above-mentioned substratum is transferred to 7,121 ℃ of sterilization 30min.Is 4% to be inoculated in the substratum photosynthetic bacterium (commercial) according to inoculum density, and the vibration mixing places the illumination box illumination cultivation, and illumination condition is 1500lux, 30 ℃ of temperature.
Enrichment culture result: illumination cultivation is after 5 days, bacterium liquid OD
660Be 1.8, illumination cultivation reached the maximum growth amount after 12 days, bacterium liquid OD
660Be 2.5, make the logarithmic phase of photosynthetic bacterium prolong 7 days, the maximum growth amount improves.
Photosynthetic bacterium with this culture medium culturing is applied to culture the water purification processing, and breed water COD, ammonia-N removal rate have reached 61%, 70.48% respectively.
Comparative Examples
Get the conventional substratum of introducing in embodiment 3 substratum of the present invention and the background technology respectively, the pH that regulates substratum respectively is 7,121 ℃ of sterilization 30min.Is 4% to be inoculated in the substratum photosynthetic bacterium (commercial) according to inoculum density, and the vibration mixing places the illumination box illumination cultivation, and illumination condition is 1500lux, 30 ℃ of temperature.Illumination cultivation 12 days is measured optical density(OD) OD in the substratum enrichment culture process
660Change (see figure 1) and pH and change (see figure 2).
As can be seen from Figure 1, use conventional culture medium culturing photosynthetic bacterium, bacterium liquid begins to change red after 3 days, bacterium liquid OD in the time of the 5th day
660Be its OD after 1.5,5 days
660Reduce gradually; Use culture medium culturing photosynthetic bacterium of the present invention, along with the increase of incubation time, bacterium liquid OD
660Increase gradually, cultivated its OD 12 days
660Be 2.4, substratum of the present invention makes the logarithmic phase of photosynthetic bacterium prolong 7 days, and its breeding is more had promoter action.
As shown in Figure 2, along with the constantly rising of growth pH value of photosynthetic bacterium, when conventional pH in culture medium value rose to 9, bacterial growth was suppressed.Use culture medium culturing photosynthetic bacterium of the present invention to rise slowly, the logarithmic phase of photosynthetic bacterium is prolonged with respect to conventional medium pH value.
Claims (2)
1, a kind of photosynthetic bacterium enriched substratum is characterized in that being made up of basic medium, yeast extract paste and hydroxy-butanedioic acid; In basic medium, every 1000ml basic medium adds the yeast extract paste of 0.1g～3.0g and the hydroxy-butanedioic acid of 0.5g～2.0g;
Wherein, described basic medium is: with ammonium chloride 1.0g, and sodium bicarbonate 1.0g, dipotassium hydrogen phosphate 0.2g, sodium acetate 3.0g, magnesium sulfate heptahydrate 0.2g, sodium-chlor 1.0g is made into 1000ml with distilled water.
2, a kind of photosynthetic bacterium enriched substratum according to claim 1 is characterized in that in basic medium, and every 1000ml basic medium adds the yeast extract paste of 1.0g and the hydroxy-butanedioic acid of 1.5g.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102732461A (en) * | 2012-06-14 | 2012-10-17 | 辽宁大学 | Acinetobacter baumannii enriched culture medium |
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| CN101886052B (en) * | 2010-06-08 | 2012-07-04 | 沈阳师范大学 | Photosynthetic bacteria enrichment culture medium |
| US9708580B2 (en) | 2012-05-15 | 2017-07-18 | Shanghai Zhiyi Information Technology Ltd. | Bacterial culture media and methods for their preparation and use |
| CN105199993A (en) * | 2015-10-22 | 2015-12-30 | 中国人民大学 | Photosynthetic bacteria culture medium and preparation method thereof |
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| 光合细菌PSB-1菌株的分离鉴定及其生物学特性的研究. 刘勇，张德咏.生命科学研究，第4卷第4期. 2000 * |
| 光合细菌乳酸代谢机制的初步研究. 谷劲松等.应用与环境生物学报，第9卷第2期. 2003 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102732461A (en) * | 2012-06-14 | 2012-10-17 | 辽宁大学 | Acinetobacter baumannii enriched culture medium |
| CN102732461B (en) * | 2012-06-14 | 2014-03-26 | 辽宁大学 | Acinetobacter baumannii enriched culture medium |
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