CN101886052B - Photosynthetic bacteria enrichment culture medium - Google Patents
Photosynthetic bacteria enrichment culture medium Download PDFInfo
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- CN101886052B CN101886052B CN2010101942083A CN201010194208A CN101886052B CN 101886052 B CN101886052 B CN 101886052B CN 2010101942083 A CN2010101942083 A CN 2010101942083A CN 201010194208 A CN201010194208 A CN 201010194208A CN 101886052 B CN101886052 B CN 101886052B
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- photosynthetic bacteria
- culture medium
- photosynthetic
- enrichment culture
- milk powder
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Abstract
The invention discloses a photosynthetic bacteria enrichment culture medium, which belongs to the technical field of biology. The culture medium contains bone marrow extract, instant soybean milk powder and other components comprising ammonium chloride, sodium acetate, sodium chloride, magnesium sulfate and dipotassium phosphate which are finally determined by screening according to the basic components of a conventional culture medium for photosynthetic bacteria. The culture medium can improve the culture efficiency of the photosynthetic bacteria, the color of bacteria liquor is brighter, and the specific peculiar smell of the photosynthetic bacteria during culture is reduced to a great extent. The quality of the photosynthetic bacteria liquid produced by adopting the culture medium is remarkably improved, and a prepared photosynthetic bacteria preparation has wider utility value. The enrichment culture medium is more suitable for development of high-quality photosynthetic bacteria preparations and related products.
Description
Technical field
The present invention relates to a kind of microbiological culture media, specifically a kind of photosynthetic bacteria enrichment culture medium belongs to biological technical field.
Background technology
Photosynthetic bacterium (Photosynthetic bacteria, be called for short PSB) is the prokaryotic organism with original luminous energy synthetic system that occur the earliest on the earth, is one type and can carries out photosynthesis and the procaryotic general name of the special physiological monoid of non-oxygen-production.Under the different natural condition; Photosynthetic bacterium has multiple functions such as fixed nitrogen, dehydrogenation, solid carbon, sulfide oxidation; It has constituted the original producer in the nature ecosystem with other photosynthetic organisms, and in nature Carbon cycle and material conversion, plays an important role.
Photosynthetic bacterial thallus itself is nontoxic, has been rich in multiple nutrients material and physiologically active substance, has the microecosystem regulation balance, promotes animal and plant growth, increases output and special physiological properties such as improve the quality.It can promote many beneficial microorganism propagation, and the symbiotic reproduction relation through multiple micropopulation is mutually promoted forms a complicacy and stable microflora.Along with people's deepens continuously to photosynthetic bacterium research, and The experimental results shows that photosynthetic bacterium is all having good using value aspect plant husbandry, aquatic products, livestock and poultry cultivation and the organic sewage processing.
For the needs that are fit to produce, research and develop out the photosynthetic bacteria culture medium that is applicable to different purposes, more help the promotion and application of photosynthetic bacterium culture technique.Disclose the conventional substratum of many photosynthetic bacteriums in the prior art, summarized to get up to mainly contain:
(1) yeast extract paste 1.5g, NH
4Cl 1.0g, MgCl
20.2g, K
2HPO
40.5g, NaCl 1.0g, NaHCO3 5.0g, water 1000mL, pH=7.0-7.2,115 ℃ of sterilization 30min.
(2) (NH
4)
2SO
41.0g, MgSO
47H2O 0.2g, NaHCO
35.0g, K
2HPO
40.5g, NaCl 0.2g, peptone 1.5g, zero(ppm) water 1000mL, pH=7.0-7.2,115 ℃ of sterilization 30min.
(3) NH
4Cl 1.0g, NaHCO
31.0g, K
2HPO
40.2g, peptone 2.0g, CH
3COONa 3.0g, MgSO
47H
2O 0.2g, NaCl 1.25g, yeast extract paste 1g, zero(ppm) water 1000mL, inorganic salt solution 1.0mL, pH=7.0,115 ℃ of sterilization 30min.
Annotate: the preparation of inorganic salt solution: FeCl
35.0mg, CuSO
40.05mg, H
3BO
4, 1.0mg, MnCl
24H
2O 0.05mg, ZnSO
47H
2O 1.0mg, Co (NO
3) 20.5mg, zero(ppm) water 1000mL.
(4) NH
4Cl 1.0g, Na
2HPO
4, 0.5g, MgCl
20.2g, NaCl2.0g, peptone 1.5g, NaHCO
35.0g, ethanol 1.5mL, zero(ppm) water 1000mL is with (the NH of 0.1N
4)
3PO
4Or Na
2CO
3Solution transfers to pH=7.0,115 ℃ of sterilization 30min.
(5) NaHCO
31.0g, CH
3COONa 3.0g, yeast extract paste 2.0g,, K
2HPO
40.5g, Fe-EDTA 0.005g, NH
4Cl 1.0g, MgCl
26H 20 0.2g, NaCl5.0g, water 1000mL, pH=7.6,115 ℃ of sterilization 30min.
But, above-mentioned these substratum also exist some perfect inadequately places, as: the photosynthetic bacterium growth cycle is long, and the specific peculiar smell that photosynthetic bacterium is produced in process of growth is bigger, and bacterium liquid color is darker etc.These problems all are unfavorable for the exploitation of high-quality photosynthetic bacteria preparation and related prods.
Summary of the invention:
The objective of the invention is deficiency to above-mentioned prior art; And a kind of photosynthetic bacterium activity that can improve is provided; Increase the bright-colored degree of bacterium liquid, reduce the specific peculiar smell that photosynthetic bacterium is produced in process of growth, be applicable to the enrichment medium of the high-quality photosynthetic bacterium product of exploitation.
Photosynthetic bacteria enrichment culture medium provided by the present invention contains two kinds of compositions of bone-marrow extract and instant soybean milk powder in this substratum, contain bone-marrow extract 0.5-1.5g in every liter of substratum, instant soybean milk powder 0.8-1.2g.Other composition is the basis with the conventional substratum of photosynthetic bacterium in the substratum, through preferably obtaining, comprises ammonium chloride, sodium acetate, sodium-chlor, sal epsom, several kinds of compositions of potassium hydrogenphosphate.Through orthogonal test, confirm that finally the photosynthetic bacteria enrichment culture medium prescription is: bone-marrow extract 0.5-1.5g, instant soybean milk powder 0.8-1.2g, NH
4Cl0.5-1.0g, CH
3COONa 1.0-2.0g, NaCl 0.5-1.0g, MgSO
40.1-0.15g, K
2HPO
40.1g, pH=6.7-7.0, water 1000mL.
Foodstuff additive-the bone-marrow extract that be used to allocate condensed soup article according to traditional Baoshang technology produced of described bone-marrow extract for being sold in the market.
Food raw material-the instant soybean milk powder that be used to make soya-bean milk of described instant soybean milk powder for being sold in the market.
Use photosynthetic bacteria enrichment culture medium and carry out the photosynthetic bacterium cultured method: with the photosynthetic bacteria enrichment culture medium for preparing through 121 ℃ sterilized 20 minutes after; Inoculum size with 5-30% inserts cultured photosynthetic bacteria liquid; At 27-30 ℃; Cultivate under the 3000-5000Lux condition, stir or rock 1-2 time every day, cultivates 2-5 angel photosynthetic bacterium number and reach 2 * 10
8More than/the mL.
Advantage of the present invention and effect:
1, improves the number of viable of photosynthetic bacterium, shorten the growth cycle of photosynthetic bacterium.Adopt substratum provided by the invention, carry out enrichment culture, OD after 48 hours with 5% inoculum size
660Value increased rapidly, at 120 hours OD
660Value reaches climax 2.1, and bacterial concentration slightly descends afterwards, but still keeps the higher concentration level.
2, bacterium liquid color is more bright-coloured.Adopting the photosynthetic bacteria liquid color that the present invention cultivated is bright red, and to compare color more bright-coloured with other cultural method at present.
3, the peculiar peculiar smell of photosynthetic bacterium has had reduction significantly in culturing process.
The quality of the photosynthetic bacteria liquid of 4, being produced has had raising more significantly.Photosynthetic bacteria enrichment culture medium provided by the present invention; Select for use food grade additives as raw material; The quality of the photosynthetic bacteria preparation of being produced has had and has significantly improved, and is applicable to the exploitation of high-quality photosynthetic bacteria preparation and related prods, has practical value widely.
Embodiment:
Below in conjunction with specific embodiment the present invention is elaborated:
The method of embodiment 1 photosynthetic bacterium enrichment culture
(1) preparation of photosynthetic bacteria enrichment culture medium: bone-marrow extract 0.5g, instant soybean milk powder 0.8g, NH
4Cl 0.8g, CH
3COONa 1.5g, NaCl 0.5g, MgSO
40.15g, K
2HPO
40.1g, pH=6.7, water 1000mL.
(2) photosynthetic bacterium enrichment culture: above-mentioned prepared culture medium at 121 ℃ of 30min that sterilize down, is got cultured photosynthetic bacterium seed liquor, and the ratio with 5% is inoculated in the substratum; Place 27 ℃; Under the 3000-5000Lux condition, anaerobism was cultivated after 5 days, reached the maximum growth amount; Nutrient solution is bright red, measures OD
660=1.94, viable count is 2.7 * 10 in the employing tube dilution method mensuration photosynthetic bacterium nutrient solution
9
The method of embodiment 2 photosynthetic bacterium high-level efficiency enrichment culture
(1) preparation of photosynthetic bacteria enrichment culture medium: bone-marrow extract 1.2g, instant soybean milk powder 1.0g, NH
4Cl 1.0g, CH
3COONa 2.0g, NaCl 0.5g, MgSO
40.15g, K
2HPO
40.1g, pH=6.7, water 1000mL.
(2) photosynthetic bacterium enrichment culture: above-mentioned prepared culture medium at 121 ℃ of 30min that sterilize down, is got cultured photosynthetic bacterium seed liquor, and the ratio with 30% is inoculated in the substratum; Place 27 ℃; Under the 3000-5000Lux condition, anaerobism was cultivated after 2 days, reached the maximum growth amount; Nutrient solution is bright red, measures OD
660=2.4, viable count is 3.47 * 10 in the employing tube dilution method mensuration photosynthetic bacterium nutrient solution
9
Above-mentioned photosynthetic bacterium culture condition is with 28-30 ℃, and 4000-5000Lux is advisable.
The concrete prescription of embodiment 3 photosynthetic bacteria enrichment culture mediums: bone-marrow extract 0.5g; Instant soybean milk powder 1.0g; NH
4Cl 0.5g; CH
3COONa 1.5g; NaCl 0.5g; MgSO
40.1g; K
2HPO
40.1g, pH=6.7-7.0, water 1000mL.
The concrete prescription of embodiment 4 photosynthetic bacteria enrichment culture mediums: bone-marrow extract 1.0g; Instant soybean milk powder 0.8g; NH
4Cl 0.8g; CH
3COONa 1g; NaCl 0.8g; MgSO
40.15g; K
2HPO
40.1g, pH=6.7-7.0, water 1000mL.
The concrete prescription of embodiment 5 photosynthetic bacteria enrichment culture mediums: bone-marrow extract 1.5g; Instant soybean milk powder 1.2g; NH
4Cl 1.0g; CH
3COONa 2.0g; NaCl 1.0g; MgSO
40.15g; K
2HPO
40.1g, pH=6.7-7.0, water 1000mL.
Embodiment 6 application of photosynthetic bacterium nutrient solution in breeding layer chicken
(1) selection of experimental subjects: choose the laying hen raised in two identical hen houses of same laying hen field structure as experimental subjects, layer breed is the blue brown laying hen in sea.6534 of experimental group livestock on hands, 13 monthly ages; 6124 of control group livestock on hands, age in December.Experimental group and the control group laying hen same feed of all feeding, other way to manages are consistent.
(2) experimental technique: with embodiment 2 prepared photosynthetic bacterium nutrient solutions the experimental group laying hen is added in the drinking-water with 1: 200 ratio and to feed, control group does not add bacterium liquid.The bacterium liquid that adds required used up the same day, and other raising conditions are constant, and experimental period is 4 months.
(3) experimental result: experimental group is adding after bacterium liquid fed 5 days, and food consumption increases by 3.4% than in the past, experimental session, and experimental group average per thousand chicken egg productivities of comparing with control group improve 4.7%, and broken rate of eggshell reduces by 68%; Experimental session, experimental group is morbidity not, about 500 yuan of prophylactic costs, 4200 yuan of control group experimental session morbidity medication costs; Sick chicken was 37 a little less than the experimental session experimental group was eliminated, and sick chicken was 174 a little less than control group was eliminated.
Embodiment 7 photosynthetic bacterium nutrient solutions are to the influence of growth of maize
Experimental technique: (1) with embodiment 2 made photosynthetic bacterium liquid, and to corn seed immersion treatment 12 hours, control group was soaked seed with clear water, places to cultivate after 5 days under 28 ℃ of conditions to measure percentage of germination again with 1: 500 diluent.2) seed after the immersion carries out sowing test (silty loam), when corn 4-5 in seedling stage sheet leaf, measures plant height, leaf fresh weight and the dry weight of corn seedling, indexs such as root length, root fresh weight and dry weight.
Experimental result: the corn seed through diluent seed soaking in 1: 500 is handled is compared with the corn seed that the clear water seed soaking is handled, and percentage of germination improves 3.4%, and growth of seedling phase (4-5 sheet leaf) plant height increases by 18.2%, and leaf fresh weight increases by 8.9%, and leaf dry weight increases by 6.8%; Root is long to increase by 4.1%, and the root fresh weight increases by 24.6%, and leaf dry weight increases by 17.3%.
Claims (4)
1. photosynthetic bacteria enrichment culture medium, its prescription is: bone-marrow extract 0.5-1.5g, instant soybean milk powder 0.8-1.2g, NH
4Cl 0.5-1.0g, CH
3COONa 1-2g, NaCl0.5-1.0g, MgSO
40.1-0.15g, K
2HPO
40.1g, pH=6.7-7.0, water 1000mL.
2. photosynthetic bacteria enrichment culture medium as claimed in claim 1 is characterized in that the concrete prescription of this substratum: bone-marrow extract 0.5g, instant soybean milk powder 1.0g, NH
4Cl 0.5g, CH
3COONa 1.5g, NaCl 0.5g, MgSO
40.1g, K
2HPO
40.1g, pH=6.7-7.0, water 1000mL.
3. photosynthetic bacteria enrichment culture medium as claimed in claim 1 is characterized in that the concrete prescription of this substratum: bone-marrow extract 1.0g, instant soybean milk powder 0.8g, NH
4Cl 0.8g, CH
3COONa 1g, NaCl 0.8g, MgSO
40.15g, K
2HPO
40.1g, pH=6.7-7.0, water 1000mL.
4. photosynthetic bacteria enrichment culture medium as claimed in claim 1 is characterized in that the concrete prescription of this substratum: bone-marrow extract 1.5g, instant soybean milk powder 1.2g, NH
4Cl 1.0g, CH
3COONa 2.0g, NaCl 1.0g, MgSO
40.15g, K
2HPO
40.1g, pH=6.7-7.0, water 1000mL.
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| CN101886052B true CN101886052B (en) | 2012-07-04 |
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| CN104087527B (en) * | 2014-06-20 | 2017-01-11 | 沈阳壅源生物有机肥有限公司 | Bacillus thuringiensis, and preparation method for solid medium of Bacillus thuringiensis |
| CN107232459B (en) * | 2017-06-01 | 2020-12-29 | 沈阳师范大学 | Preparation method of beverage with spleen strengthening and stomach nourishing functions |
| CN109666611A (en) * | 2019-01-28 | 2019-04-23 | 南通龙洋水产有限公司 | A kind of preparation of photosynthetic bacteria used for aquiculture and purification process |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101113418A (en) * | 2007-07-06 | 2008-01-30 | 辽宁大学 | Photosynthetic bacterium enriched culture medium |
| JP2008199914A (en) * | 2007-02-16 | 2008-09-04 | Sankootekku Kk | Liquid culture medium and method for proliferating and culturing photosynthetic bacterium |
| CN101407771A (en) * | 2008-10-31 | 2009-04-15 | 哈尔滨工业大学 | Photosynthetic bacteria culture medium |
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2010
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008199914A (en) * | 2007-02-16 | 2008-09-04 | Sankootekku Kk | Liquid culture medium and method for proliferating and culturing photosynthetic bacterium |
| CN101113418A (en) * | 2007-07-06 | 2008-01-30 | 辽宁大学 | Photosynthetic bacterium enriched culture medium |
| CN101407771A (en) * | 2008-10-31 | 2009-04-15 | 哈尔滨工业大学 | Photosynthetic bacteria culture medium |
Non-Patent Citations (2)
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| 张红莲等.富集光合细菌培养基的优化研究.《水产科学》.2005,第24卷(第7期),23-25. * |
| 陈秀丽等.光合细菌培养基配方的优化研究.《渔业现代化》.2007,第34卷(第6期),47-49. * |
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