CN101407771A

CN101407771A - Photosynthetic bacteria culture medium

Info

Publication number: CN101407771A
Application number: CNA2008101374444A
Authority: CN
Inventors: 张光明; 何春华
Original assignee: Harbin Institute of Technology
Current assignee: Harbin Institute of Technology
Priority date: 2008-10-31
Filing date: 2008-10-31
Publication date: 2009-04-15
Anticipated expiration: 2028-10-31
Also published as: CN101407771B

Abstract

The invention discloses a photosynthetic bacteria culture medium, relates to a bacteria culture medium, and solves the problems that the existing photosynthetic bacteria culture media contains high-concentration yeast extract that leads to darker lysate color and high production cost, and pH value of the culture medium rises along with the prolonging of cultivation time, thus inhibiting the growth of photosynthetic bacteria and leading to lower bacteria concentration. The photosynthetic bacteria culture medium is made of low molecular weight carbonaceous organic substances, ammonium salts, the yeast extract, MgSO4, CaCl2, K2HPO4, KH2PO4, trace elements and distilled water. As the photosynthetic bacteria culture medium takes the low molecular weight carbonaceous organic substances that do not contain Na<+> as carbon source, pH value of the culture medium does not rise along with the prolonging of cultivation time, and the growth of photosynthetic bacteria is not inhibited; low-concentration yeast extract is adopted to reduce production cost, and the trace elements ensure bright photosynthetic bacteria lysate color; photosynthetic bacteria cultivated by the culture medium has the exponential growth phase of two days, thus having high bacteria concentration and the OD660 of 1.8.

Description

A kind of photosynthetic bacteria culture medium

Technical field

The present invention relates to a kind of bacteria culture medium.

Background technology

Photosynthetic bacterium is that a class can be carried out photosynthesis and the procaryotic general name of the special physiological monoid of non-oxygen-production belongs to the class in the hydrosphere microorganism, extensively is present in the natural various water body.Photosynthetic bacterial thallus is nontoxic, is rich in nutritive substance, protein content up to 60%～65%, contain physiologically active substances such as multiple vitamin B group, pantothenic acid, ubiquinone.Photosynthetic bacterium is widely used in aquaculture, plant husbandry, aspects such as sewage disposal.Photosynthetic bacterial thallus contains abundant bacteriochlorophyll and carotenoid, and these two kinds of pigments are easy to extract, and are the new resources of natural food colour, can be used for the painted processing of butter, ice-creams, cheese, lactic drink, cake etc.

At present, yeast extract paste concentration＞2000mg/L in the traditional substratum of photosynthetic bacterium, though this can promote the growth and breeding of photosynthetic bacterium, but the yeast extract paste of high density makes bacterium liquid colour-darkening, influence the aesthetic quality of painted processing, use the yeast extract paste of high density also to increase production cost, and owing to have Na in the substratum ⁺Thereby, therefore cause the medium pH value to raise and suppress the growth of photosynthetic bacterium along with the prolongation of incubation time; Adopt traditional culture medium culturing, the logarithmic phase of photosynthetic bacterium is short, has only one day, and bacteria concentration is low, OD ₆₆₀=1.2 (optical density(OD) under the 660nm wavelength is 1.2).

Summary of the invention

Thereby the present invention seeks to exist the yeast extract paste of high density that bacterium liquid colour-darkening, production cost height, medium pH value can be raise along with the prolongation of incubation time to suppress growth and the low problem of bacteria concentration of photosynthetic bacterium, and a kind of photosynthetic bacteria culture medium is provided in order to solve existing photosynthetic bacteria culture medium.

Photosynthetic bacteria culture medium is by lower molecular weight carbonaceous organic material, ammonium salt, yeast extract paste, MgSO ₄, CaCl ₂, K ₂HPO ₄, KH ₂PO ₄, trace element and distilled water makes; Carbon content in wherein every 1L photosynthetic bacteria culture medium in the lower molecular weight carbonaceous organic material is that the nitrogen content in 0.06～4.00g/L, the ammonium salt is that 0.07～2.90g/L, yeast extract paste content are 0.10～1.00g/L, MgSO ₄Content is 0.10～0.14g/L, CaCl ₂Content is 0.73～0.77g/L, K ₂HPO ₄Content is 0.48～0.52g/L, KH ₂PO ₄Content is that 0.28～0.32g/L, micronutrient levels are the distilled water of 0.80～1.20ml/L and surplus; The lower molecular weight carbonaceous organic material is ethanol, DL-oxysuccinic acid or glucose; Ammonium salt is NH ₄Cl or (NH ₄) ₂SO ₄Trace element is Zn ²⁺, Mn ²⁺, Cu ²⁺, Co ²⁺And Fe ²⁺The pH value of substratum is 5.5～7.5.

Adopt among the present invention and do not contain Na ⁺The lower molecular weight carbonaceous organic material be carbon source, the medium pH value does not raise along with the prolongation of incubation time, remains at 5.5～7.5, can not suppress the growth of photosynthetic bacterium, adopts the yeast extract paste of lower concentration to reduce production cost simultaneously yet; Trace element can not only satisfy the demand of photosynthetic bacterium growth, and can guarantee that the bacterium liquid of photosynthetic bacterium is bright-colored; Adopt the culture medium culturing among the present invention, the logarithmic phase of photosynthetic bacterium reaches two days, bacteria concentration height, OD ₆₆₀=1.8, the photosynthetic bacterium behind the cultivation 24h increases multiple and reaches 14.8, only is 14.1 and adopt the photosynthetic bacterium growth multiple of traditional culture medium culturing.

Description of drawings

Fig. 1 is the growth curve collection of illustrative plates of photosynthetic bacterium in the embodiment four, wherein ◆ the growth curve of the photosynthetic bacterium of the photosynthetic bacteria culture medium cultivation among the present invention is adopted in expression, and the growth curve of the photosynthetic bacterium of traditional RCVBN culture medium culturing is adopted in ▲ expression.

Embodiment

Technical solution of the present invention is not limited to following cited embodiment, also comprises the arbitrary combination between each embodiment.

Embodiment one: the present embodiment photosynthetic bacteria culture medium is by lower molecular weight carbonaceous organic material, ammonium salt, yeast extract paste, MgSO ₄, CaCl ₂, K ₂HPO ₄, KH ₂PO ₄, trace element and distilled water makes; Carbon content in wherein every 1L photosynthetic bacteria culture medium in the lower molecular weight carbonaceous organic material is that the nitrogen content in 0.06～4.00g/L, the ammonium salt is that 0.07～2.90g/L, yeast extract paste content are 0.10～1.00g/L, MgSO ₄Content is 0.10～0.14g/L, CaCl ₂Content is 0.73～0.77g/L, K ₂HPO ₄Content is 0.48～0.52g/L, KH ₂PO ₄Content is that 0.28～0.32g/L, micronutrient levels are the distilled water of 0.80～1.20ml/L and surplus; The lower molecular weight carbonaceous organic material is ethanol, DL-oxysuccinic acid or glucose; Ammonium salt is NH ₄Cl or (NH ₄) ₂SO ₄Trace element is Zn ²⁺, Mn ²⁺, Cu ²⁺, Co ²⁺And Fe ²⁺The pH value of substratum is 5.5～7.5.

Adopt in the present embodiment and do not contain Na ⁺The lower molecular weight carbonaceous organic material be carbon source, the medium pH value can not raise along with the prolongation of incubation time, remains at 5.5～7.5, can not suppress the growth of photosynthetic bacterium.

Embodiment two: present embodiment and embodiment one are different is that the carbon content in the lower molecular weight carbonaceous organic material is that nitrogen content in 1.00g/L, the ammonium salt is that 2.00g/L, yeast extract paste content are 0.50g/L, MgSO in every 1L photosynthetic bacteria culture medium ₄Content is 0.12g/L, CaCl ₂Content is 0.75g/L, K ₂HPO ₄Content is 0.50g/L, KH ₂PO ₄Content is that 0.30g/L, micronutrient levels are the distilled water of 1.00ml/L and surplus.Other is identical with embodiment one.

Trace element can not only satisfy the demand of photosynthetic bacterium growth in the present embodiment, and can guarantee that the bacterium liquid of photosynthetic bacterium is bright-colored.

Embodiment three: that present embodiment and embodiment one are different is Zn in the trace element ²⁺Concentration is 0.001～0.005mol/L, Mn ²⁺Concentration is 0.005～0.015mol/L, Cu ²⁺Concentration is 0.001～0.005mol/L, Co ²⁺Concentration is 5.00 * 10 ^-4～10.00 * 10 ^-4Mol/L, Fe ²⁺Concentration is 0.001～0.005mol/L.Other step is identical with embodiment one.

Embodiment four: the present embodiment photosynthetic bacteria culture medium is by lower molecular weight carbonaceous organic material, ammonium salt, yeast extract paste, MgSO ₄, CaCl ₂, K ₂HPO ₄, KH ₂PO ₄, trace element and distilled water makes; Carbon content in wherein every 1L photosynthetic bacteria culture medium in the lower molecular weight carbonaceous organic material is that the nitrogen content in 1.00g/L, the ammonium salt is that 2.00g/L, yeast extract paste content are 0.50g/L, MgSO ₄Content is 0.12g/L, CaCl ₂Content is 0.75g/L, K ₂HPO ₄Content is 0.50g/L, KH ₂PO ₄Content is that 0.30g/L, micronutrient levels are the distilled water of 1.00ml/L and surplus; The lower molecular weight carbonaceous organic material is the DL-oxysuccinic acid; Ammonium salt is NH ₄Cl; Trace element is Zn ²⁺, Mn ²⁺, Cu ²⁺, Co ²⁺And Fe ²⁺The pH value of substratum is 6～7.

Photosynthetic bacteria culture medium in the present embodiment is detecting after cultivating photosynthetic bacterium 24h under the identical condition with traditional RCVBN substratum, the result as shown in Figure 1, photosynthetic bacterium logarithmic phase in the photosynthetic bacteria culture medium in the present embodiment was Duoed one day than the photosynthetic bacterium logarithmic phase in traditional RCVBN substratum, and the bacteria concentration height, OD ₆₆₀=1.8.

Claims

1, a kind of photosynthetic bacteria culture medium is characterized in that photosynthetic bacteria culture medium is by lower molecular weight carbonaceous organic material, ammonium salt, yeast extract paste, MgSO ₄, CaCl ₂, K ₂HPO ₄, KH ₂PO ₄, trace element and distilled water makes; Carbon content in wherein every 1L photosynthetic bacteria culture medium in the lower molecular weight carbonaceous organic material is that the nitrogen content in 0.06～4.00g/L, the ammonium salt is that 0.07～2.90g/L, yeast extract paste content are 0.10～1.00g/L, MgSO ₄Content is 0.10～0.14g/L, CaCl ₂Content is 0.73～0.77g/L, K ₂HPO ₄Content is 0.48～0.52g/L, KH ₂PO ₄Content is that 0.28～0.32g/L, micronutrient levels are the distilled water of 0.80～1.20ml/L and surplus; The lower molecular weight carbonaceous organic material is ethanol, DL-oxysuccinic acid or glucose; Ammonium salt is NH ₄Cl or (NH ₄) ₂SO ₄Trace element is Zn ²⁺, Mn ²⁺, Cu ²⁺, Co ²⁺And Fe ²⁺The pH value of substratum is 5.5～7.5.

2, a kind of photosynthetic bacteria culture medium according to claim 1 is characterized in that the carbon content in the lower molecular weight carbonaceous organic material in every 1L photosynthetic bacteria culture medium is that nitrogen content in 1.00g/L, the ammonium salt is that 2.00g/L, yeast extract paste content are 0.50g/L, MgSO ₄Content is 0.12g/L, CaCl ₂Content is 0.75g/L, K ₂HPO ₄Content is 0.50g/L, KH ₂PO ₄Content is that 0.30g/L, micronutrient levels are the distilled water of 1.00ml/L and surplus.

3, a kind of photosynthetic bacteria culture medium according to claim 1 is characterized in that Zn in the trace element ²⁺Concentration is 0.001～0.005mol/L, Mn ²⁺Concentration is 0.005～0.015mol/L, Cu ²⁺Concentration is 0.001～0.005mol/L, Co ²⁺Concentration is 5.00 * 10 ^-4～10.00 * 10 ^-4Mol/L, Fe ²⁺Concentration is 0.001～0.005mol/L.