CN103436472A - Composite micro-ecological preparation for improving pond water quality - Google Patents
Composite micro-ecological preparation for improving pond water quality Download PDFInfo
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Abstract
The invention provides a composite micro-ecological preparation for improving the pond water quality. A preparation method of the composite micro-ecological preparation specifically comprises the following steps of activating rhodopseudomonas palustris, nitrifying bacteria, lactobacillus and saccharomyces cerevisiae in a freezing preservation tube respectively in a slant; purifying to obtain slant strains for later use; preparing the corresponding original bacterial liquid by use of each slant strain; inoculating the original bacterial liquids at a bacterial amount ratio of 2:2:1:1 in a sterilized fermentation medium I for culturing to obtain a first-level seed of a composite micro-ecological microbial inoculum; inoculating the first-level seed at an inoculation amount of 5% in a sterilized fermentation medium II for culturing to obtain a second-level seed of the composite micro-ecological microbial inoculum; and finally, inoculating the second-level seed at an inoculation amount of 5% in a sterilized fermentation medium III for culturing to obtain a finished product of the composite micro-ecological microbial inoculum. By adopting the composite micro-ecological preparation provided by the invention, the dissolved oxygen in a water body can be increased, and the COD in the water body can be reduced so as to realize an effect of improving and optimizing the pond water quality.
Description
[technical field]
The present invention relates to the compound micro-ecological preparation that a kind of aquaculture field is used, relate in particular to a kind of compound micro-ecological preparation for improvement of pond water quality.
[background technology]
Since reform and opening-up, the culture fishery of China has obtained the development of advancing by leaps and bounds, and becomes the fishery big country that unique cultured output in the world surpasses fishery output.In the culture fishery of China, pond aquaculture has occupied very important status, and it is traditional aquaculture model of technical characterictic that existing pond aquaculture has acted on and take " close, mixed, wheel " mostly.In order to improve the cultured output of unit of water body, high yield covets, undue increase water nutrition often, excessively bait throwing in, fertilising and increasing breeding density, cause the too fast breeding of harmful microorganism in water, harmful algae, and occur that movement and residual bait pollute, the ecology of destruction aquaculture water is micro-ecological environment especially, thereby endanger directly or indirectly the aquaculture organism in water body, and then make the cultivation of high-quality aquatic products kind be subject to the restriction of water quality.Water ecological setting is destroyed, and in pond, the disease of aquatic animal occurs frequently, and fishery is sustained a great loss.In order to prevent or prevent disease, at present in the whole culture-cycle all using the fish medicine, although the use of fish medicine obtains the effect of emergence therapeutic, brought more such as resistance, in fishery products and environment, accumulated the problems such as residual.Therefore, from ecological angle, although cultivating pool is a very complicated aquatic ecosystem, no matter how complicated it is, be the problem of an environment after all.The control of cultivating pool water ecological setting has become the key issue of development China pond aquaculture, therefore purification and the improvement of water quality of aquaculture pond is seemed to very important.
[summary of the invention]
Technical problem to be solved by this invention is to provide a kind of compound micro-ecological preparation for improvement of pond water quality.
The present invention solves the problems of the technologies described above by the following technical programs: a kind of compound micro-ecological preparation for improvement of pond water quality, and the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: by the Rhodopseudomonas palustris in the freezing pipe, nitrifier, lactobacillus, and yeast saccharomyces cerevisiae in inclined-plane, activate respectively, be placed in respectively afterwards the 90mm culture dish and carry out purifying, obtain Rhodopseudomonas palustris slant strains, nitrifier slant strains, lactobacillus slant strains, reach the yeast saccharomyces cerevisiae slant strains, standby;
(2) former bacterial classification liquid culture: the Rhodopseudomonas palustris slant strains is inoculated in sterilized liquid nutrient medium I, obtains the Rhodopseudomonas palustris original bacteria liquid in 5-7 days in 28 ℃ of-30 ℃ of anaerobism tengsten lamp illumination cultivation afterwards; The nitrifier slant strains is inoculated in sterilized liquid nutrient medium II, and then under 25 ℃-28 ℃, aerobic cultivation obtains the nitrifier original bacteria liquid in 3-5 days; The lactobacillus slant strains is inoculated in sterilized liquid nutrient medium III, then in 37 ℃ of lower anaerobism, cultivates and within 1-2 days, obtain the lactobacillus original bacteria liquid; The yeast saccharomyces cerevisiae slant strains is inoculated in sterilized liquid nutrient medium IV, and under 28 ℃-30 ℃, aerobic cultivation obtains the yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: by Rhodopseudomonas palustris original bacteria liquid, nitrifier original bacteria liquid, lactobacillus original bacteria liquid, and the yeast saccharomyces cerevisiae original bacteria liquid be inoculated in sterilized fermention medium I and cultivated by the bacterium amount ratio of 2:2:1:1, particularly: first inoculate nitrifier original bacteria liquid, yeast saccharomyces cerevisiae original bacteria liquid and aerobic cultivation 3 days under 28 ℃-30 ℃, and then inoculate Rhodopseudomonas palustris original bacteria liquid, 37 ℃ of anaerobism cultivations of lactobacillus original bacteria liquid 3 days, obtain the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: the first order seed of gained is inoculated in sterilized fermention medium II by 5% inoculum size, and under 28 ℃-30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: the secondary seed of gained is seeded in sterilized fermention medium III by 5% inoculum size, and under 28 ℃-30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the finished product of described compound micro-ecological preparation.
Further, the concrete operations that in described step (1), Rhodopseudomonas palustris activates in inclined-plane are: with transfering loop, Rhodopseudomonas palustris is inoculated on sterilized slant medium I, afterwards in 28 ℃-30 ℃ anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 15min; And the component of this slant medium I: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, agar 20 grams, water 1000ml, PH 7.0-7.2.
Further, the concrete operations that in described step (1), nitrifier activates in inclined-plane are: prepare the slant medium II, and the slant medium II is placed in to 121 ℃ of lower sterilizing 20min, with transfering loop, nitrifier is inoculated on the slant medium II afterwards, then aerobic cultivation 3-5 days under 25 ℃-28 ℃; The component of described slant medium II: SODIUMNITRATE 1 gram, sal epsom 0.03 gram, manganous sulfate 0.01, dipotassium hydrogen phosphate 0.8 gram, sodium carbonate 1 gram, calcium superphosphate 0.25 gram, agar 50 grams, water 1000ml, PH nature.
Further, the concrete operations that in described step (1), lactobacillus activates in inclined-plane are: with transfering loop, lactobacillus is inoculated on sterilized slant medium III, cultivates 1-2 days in 37 ℃ of lower anaerobism afterwards; The sterilising temp of described slant medium III is that 121 ℃, sterilization time are 20min; And the component of this slant medium III: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1ml, dipotassium hydrogen phosphate 2 grams, sal epsom 0.58 gram, manganous sulfate 0.28 gram, agar 15 grams, water 1000ml, PH6.5.
Further, the concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in described step (1) are: with transfering loop, yeast saccharomyces cerevisiae is inoculated on sterilized slant medium IV to aerobic cultivation 3 days under 28-30 ℃ afterwards; The sterilising temp of described slant medium IV is that 121 ℃, sterilization time are 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
Further, the component of liquid nutrient medium I in described step (2): yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium II: SODIUMNITRATE 1 gram, sal epsom 0.03 gram, manganous sulfate 0.01, dipotassium hydrogen phosphate 0.8 gram, sodium carbonate 1 gram, calcium superphosphate 0.25 gram, water 1000ml, PH nature; The component of liquid nutrient medium III: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1ml, dipotassium hydrogen phosphate 2 grams, sal epsom 0.58 gram, manganous sulfate 0.28 gram, water 1000ml, PH6.5; The component of liquid nutrient medium IV: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature.
Further, the component of fermention medium I in described step (3): tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
Further, the component of fermention medium II in described step (4): tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
Further, the component of fermention medium III in described step (5): tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
The beneficial effect of a kind of compound micro-ecological preparation for improvement of pond water quality of the present invention is: adopt this compound micro-ecological preparation can either improve the dissolved oxygen amount in water body, promote normal microflora and useful algae activation growth in breeding ecological system, keep the eubiosis of aquaculture water; Can make again movement and the pollution of residual bait in pond be purified, stablize and improve water quality, thereby having reduced the COD in the water body; It is the effect that compound micro-ecological preparation of the present invention has improvement, optimization pond water quality.
[embodiment]
A kind of compound micro-ecological preparation for improvement of pond water quality of the present invention, the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: by the Rhodopseudomonas palustris in the freezing pipe, nitrifier, lactobacillus, and yeast saccharomyces cerevisiae in inclined-plane, activate respectively, be placed in respectively afterwards the 90mm culture dish and carry out purifying, obtain Rhodopseudomonas palustris slant strains, nitrifier slant strains, lactobacillus slant strains, reach the yeast saccharomyces cerevisiae slant strains, standby;
(2) former bacterial classification liquid culture: the Rhodopseudomonas palustris slant strains is inoculated in sterilized liquid nutrient medium I, obtains the Rhodopseudomonas palustris original bacteria liquid in 5-7 days in 28 ℃ of-30 ℃ of anaerobism tengsten lamp illumination cultivation afterwards; The nitrifier slant strains is inoculated in sterilized liquid nutrient medium II, and then under 25 ℃-28 ℃, aerobic cultivation obtains the nitrifier original bacteria liquid in 3-5 days; The lactobacillus slant strains is inoculated in sterilized liquid nutrient medium III, then in 37 ℃ of lower anaerobism, cultivates and within 1-2 days, obtain the lactobacillus original bacteria liquid; The yeast saccharomyces cerevisiae slant strains is inoculated in sterilized liquid nutrient medium IV, and under 28 ℃-30 ℃, aerobic cultivation obtains the yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: by Rhodopseudomonas palustris original bacteria liquid, nitrifier original bacteria liquid, lactobacillus original bacteria liquid, and the yeast saccharomyces cerevisiae original bacteria liquid be inoculated in sterilized fermention medium I and cultivated by the bacterium amount ratio of 2:2:1:1, particularly: first inoculate nitrifier original bacteria liquid, yeast saccharomyces cerevisiae original bacteria liquid and aerobic cultivation 3 days under 28 ℃-30 ℃, and then inoculate Rhodopseudomonas palustris original bacteria liquid, 37 ℃ of anaerobism cultivations of lactobacillus original bacteria liquid 3 days, obtain the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: the first order seed of gained is inoculated in sterilized fermention medium II by 5% inoculum size, and under 28 ℃-30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: the secondary seed of gained is seeded in sterilized fermention medium III by 5% inoculum size, and under 28 ℃-30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the finished product of described compound micro-ecological preparation.
Wherein: the concrete operations that in step (1), Rhodopseudomonas palustris activates in inclined-plane are: with transfering loop, Rhodopseudomonas palustris is inoculated on sterilized slant medium I, afterwards in 28 ℃-30 ℃ anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 15min; And the component of this slant medium I: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, agar 20 grams, water 1000ml, PH 7.0-7.2.The concrete operations that in step (1), nitrifier activates in inclined-plane are: prepare the slant medium II, and the slant medium II is placed in to 121 ℃ of lower sterilizing 20min, with transfering loop, nitrifier is inoculated on the slant medium II afterwards, then aerobic cultivation 3-5 days under 25 ℃-28 ℃; The component of described slant medium II: SODIUMNITRATE 1 gram, sal epsom 0.03 gram, manganous sulfate 0.01, dipotassium hydrogen phosphate 0.8 gram, sodium carbonate 1 gram, calcium superphosphate 0.25 gram, agar 50 grams, water 1000ml, PH nature.The concrete operations that in step (1), lactobacillus activates in inclined-plane are: with transfering loop, lactobacillus is inoculated on sterilized slant medium III, cultivates 1-2 days in 37 ℃ of lower anaerobism afterwards; The sterilising temp of described slant medium III is that 121 ℃, sterilization time are 20min; And the component of this slant medium III: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1ml, dipotassium hydrogen phosphate 2 grams, sal epsom 0.58 gram, manganous sulfate 0.28 gram, agar 15 grams, water 1000ml, PH6.5.The concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in step (1) are: with transfering loop, yeast saccharomyces cerevisiae is inoculated on sterilized slant medium IV to aerobic cultivation 3 days under 28-30 ℃ afterwards; The sterilising temp of described slant medium IV is that 121 ℃, sterilization time are 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
The component of liquid nutrient medium I in step (2): yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium II: SODIUMNITRATE 1 gram, sal epsom 0.03 gram, manganous sulfate 0.01, dipotassium hydrogen phosphate 0.8 gram, sodium carbonate 1 gram, calcium superphosphate 0.25 gram, water 1000ml, PH nature; The component of liquid nutrient medium III: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1ml, dipotassium hydrogen phosphate 2 grams, sal epsom 0.58 gram, manganous sulfate 0.28 gram, water 1000ml, PH6.5; The component of liquid nutrient medium IV: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature.
The component of fermention medium I in step (3): tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.The component of fermention medium II in step (4): tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.The component of fermention medium III in step (5): tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
It should be noted that, in the present invention, the per-cent of each substratum is mass percent.
Compound micro-ecological preparation of the present invention is for improvement of the water quality of optimizing pond, and the applicant illustrates that by following experiment this compound micro-ecological preparation can play the optimization pond water quality, particularly:
In certain aquafarm, get two ponds, the area in this two pond, the depth of water, aquaculture model, stocking rate, support equipment, and the configuration such as feeding and management condition all identical, and two one, ponds are set to test tank, another is set to contrast pond, use compound micro-ecological preparation of the present invention in test tank, and amount of application is 400ml/ mu, and the contrast pond is that compound micro-ecological preparation of the present invention is not used in the blank pond; Test period July 20 to July 30; Test-results: after testing, test tank is than the contrast pond, and the dissolved oxygen amount in its water body has improved from 3.2mg/L brings up to 5.1mg/L, has improved 59%; COD drops to 1700mg/L from 2500mg/L, has reduced by 32%.Therefore, known by this test-results, adopt compound micro-ecological preparation of the present invention can optimize the water quality of water body in pond, the ecotope of balance aquaculture water, thereby the healthy growth of aquaculture organism in the promotion pond.
To sum up, adopt this compound micro-ecological preparation can either improve the dissolved oxygen amount in water body, promote normal microflora and useful algae activation growth in breeding ecological system, keep the eubiosis of aquaculture water; Can make again movement and the pollution of residual bait in pond be purified, stablize and improve water quality, thereby having reduced the COD in the water body; It is the effect that compound micro-ecological preparation of the present invention has improvement, optimization pond water quality.
Claims (9)
1. the compound micro-ecological preparation for improvement of pond water quality, it is characterized in that: the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: by the Rhodopseudomonas palustris in the freezing pipe, nitrifier, lactobacillus, and yeast saccharomyces cerevisiae in inclined-plane, activate respectively, be placed in respectively afterwards the 90mm culture dish and carry out purifying, obtain Rhodopseudomonas palustris slant strains, nitrifier slant strains, lactobacillus slant strains, reach the yeast saccharomyces cerevisiae slant strains, standby;
(2) former bacterial classification liquid culture: the Rhodopseudomonas palustris slant strains is inoculated in sterilized liquid nutrient medium I, obtains the Rhodopseudomonas palustris original bacteria liquid in 5-7 days in 28 ℃ of-30 ℃ of anaerobism tengsten lamp illumination cultivation afterwards; The nitrifier slant strains is inoculated in sterilized liquid nutrient medium II, and then under 25 ℃-28 ℃, aerobic cultivation obtains the nitrifier original bacteria liquid in 3-5 days; The lactobacillus slant strains is inoculated in sterilized liquid nutrient medium III, then in 37 ℃ of lower anaerobism, cultivates and within 1-2 days, obtain the lactobacillus original bacteria liquid; The yeast saccharomyces cerevisiae slant strains is inoculated in sterilized liquid nutrient medium IV, and under 28 ℃-30 ℃, aerobic cultivation obtains the yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: by Rhodopseudomonas palustris original bacteria liquid, nitrifier original bacteria liquid, lactobacillus original bacteria liquid, and the yeast saccharomyces cerevisiae original bacteria liquid be inoculated in sterilized fermention medium I and cultivated by the bacterium amount ratio of 2:2:1:1, particularly: first inoculate nitrifier original bacteria liquid, yeast saccharomyces cerevisiae original bacteria liquid and aerobic cultivation 3 days under 28 ℃-30 ℃, and then inoculate Rhodopseudomonas palustris original bacteria liquid, 37 ℃ of anaerobism cultivations of lactobacillus original bacteria liquid 3 days, obtain the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: the first order seed of gained is inoculated in sterilized fermention medium II by 5% inoculum size, and under 28 ℃-30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: the secondary seed of gained is seeded in sterilized fermention medium III by 5% inoculum size, and under 28 ℃-30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the finished product of described compound micro-ecological preparation.
2. a kind of compound micro-ecological preparation for improvement of pond water quality according to claim 1, it is characterized in that: the concrete operations that in described step (1), Rhodopseudomonas palustris activates in inclined-plane are: with transfering loop, Rhodopseudomonas palustris is inoculated on sterilized slant medium I, afterwards in 28 ℃-30 ℃ anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 15min; And the component of this slant medium I: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, agar 20 grams, water 1000ml, PH 7.0-7.2.
3. a kind of compound micro-ecological preparation for improvement of pond water quality according to claim 1, it is characterized in that: the concrete operations that in described step (1), nitrifier activates in inclined-plane are: prepare the slant medium II, and the slant medium II is placed in to 121 ℃ of lower sterilizing 20min, with transfering loop, nitrifier is inoculated on the slant medium II afterwards, then aerobic cultivation 3-5 days under 25 ℃-28 ℃; The component of described slant medium II: SODIUMNITRATE 1 gram, sal epsom 0.03 gram, manganous sulfate 0.01, dipotassium hydrogen phosphate 0.8 gram, sodium carbonate 1 gram, calcium superphosphate 0.25 gram, agar 50 grams, water 1000ml, PH nature.
4. a kind of compound micro-ecological preparation for improvement of pond water quality according to claim 1, it is characterized in that: the concrete operations that in described step (1), lactobacillus activates in inclined-plane are: with transfering loop, lactobacillus is inoculated on sterilized slant medium III, cultivates 1-2 days in 37 ℃ of lower anaerobism afterwards; The sterilising temp of described slant medium III is that 121 ℃, sterilization time are 20min; And the component of this slant medium III: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1ml, dipotassium hydrogen phosphate 2 grams, sal epsom 0.58 gram, manganous sulfate 0.28 gram, agar 15 grams, water 1000ml, PH6.5.
5. a kind of compound micro-ecological preparation for improvement of pond water quality according to claim 1, it is characterized in that: the concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in described step (1) are: with transfering loop, yeast saccharomyces cerevisiae is inoculated on sterilized slant medium IV to aerobic cultivation 3 days under 28-30 ℃ afterwards; The sterilising temp of described slant medium IV is that 121 ℃, sterilization time are 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
6. a kind of compound micro-ecological preparation for improvement of pond water quality according to claim 1, is characterized in that: the component of liquid nutrient medium I in described step (2): yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, agar 20 grams, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium II: SODIUMNITRATE 1 gram, sal epsom 0.03 gram, manganous sulfate 0.01, dipotassium hydrogen phosphate 0.8 gram, sodium carbonate 1 gram, calcium superphosphate 0.25 gram, water 1000ml, PH nature; The component of liquid nutrient medium III: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1ml, dipotassium hydrogen phosphate 2 grams, sal epsom 0.58 gram, manganous sulfate 0.28 gram, water 1000ml, PH6.5; The component of liquid nutrient medium IV: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature.
7. a kind of compound micro-ecological preparation for improvement of pond water quality according to claim 1, it is characterized in that: the component of fermention medium I in described step (3): tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
8. a kind of compound micro-ecological preparation for improvement of pond water quality according to claim 1, it is characterized in that: the component of fermention medium II in described step (4): tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
9. a kind of compound micro-ecological preparation for improvement of pond water quality according to claim 1, it is characterized in that: the component of fermention medium III in described step (5): tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
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| CN106818543B (en) * | 2015-12-01 | 2021-05-11 | 福建大北农水产科技有限公司 | Ecological polyculture method for grass carp and tilapia |
| CN107164265A (en) * | 2017-06-05 | 2017-09-15 | 南宁学院 | A kind of probiotics and preparation method thereof |
| CN109251874A (en) * | 2018-09-25 | 2019-01-22 | 厦门惠盈动物科技有限公司 | A kind of probiotics preparation and its preparation method and application |
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| CN103436472B (en) | 2015-09-09 |
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