CN105861367A - Pig manure methane fermenting agent formula and preparation method thereof - Google Patents

Pig manure methane fermenting agent formula and preparation method thereof Download PDF

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Publication number
CN105861367A
CN105861367A CN201610248472.8A CN201610248472A CN105861367A CN 105861367 A CN105861367 A CN 105861367A CN 201610248472 A CN201610248472 A CN 201610248472A CN 105861367 A CN105861367 A CN 105861367A
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solution
methane
leaven
marsh gas
preparation
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罗增桂
黄�焕
吴永升
黄鑫
黄星群
黄华
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Luchuan Fengyuan Agriculture And Animal Husbandry Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F11/00—Treatment of sludge; Devices therefor
    • C02F11/02—Biological treatment
    • C02F11/04—Anaerobic treatment; Production of methane by such processes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

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Abstract

The invention relates to a pig manure methane fermenting agent formula, prepared from the following components: crop straw, biogas slurry, molasses, rice bran, fresh flaccid knotweed herb, clear water, 20 percent of manganous sulphate solution, 1mg/ml of CoCl2.6H2O solution, 2mg/ml of NiCl2.6H2O solution and microorganism bacterium agent. The invention also discloses a preparation method of a pig manure methane fermenting agent. The pig manure methane fermenting agent has the advantages that after the fermenting agent is added in methane, carbon and nitrogen sources and microelements in a fermenting system can achieve reasonable values for microbial flora growth in a methane tank, the growth of methane fermenting bacterium in the methane tank is promoted, and the methane fermenting system is stable in operation; through adding exogenous microorganisms for synergistic fermentation, the utilization ratio of raw materials by the microorganisms is improved, and the effect of improve a methane production rate is achieved.

Description

A kind of swine waste marsh gas leaven formula and preparation method thereof
Technical field
The invention belongs to technical field of microbial fermentation, be specifically related to a kind of swine waste marsh gas fermentation Agent prescription and preparation method thereof.
Background technology
Along with scale of raising pigs, the improving constantly of intensive degree, the row of pig manure in unit are High-volume big, surrounding enviroment are polluted, therefore, with pig manure as raw material, passes through biogas fermentation It is one of a kind of the most conventional stool detection method that antibacterial carry out fermentation to produce biogas. In suitable temperature, PH environment, it is to utilize that biogas fermentation antibacterial consumes the speed of carbon 25～30 times of the speed of nitrogen element, therefore enter pond raw material carbon-nitrogen ratio and reach 25～30:1, Biogas fermentation can be made to be normally carried out.If carbon-nitrogen ratio is lacked of proper care, biogas fermentation antibacterial will be made Vital movement and methane tank gas production amount are affected, and fermentation raw material is not fully utilized and causes Waste.Carbon-nitrogen ratio in pig manure is about 13:1, and the most simple carries out natural pond with pig manure for raw material Gas ferments, and fermentation can be made abnormal, and gas production is low, and pig manure can not get fully fermenting, and produces and dislikes Surrounding enviroment are polluted by stink.The current existing abnormal method of solution biogas fermentation It is by adding straw, the carbon-nitrogen ratio of regulation fermentation raw material, make biogas fermentation be normally carried out, and Obtain certain improvement effect, but still have greater room for improvement.
The growth of biogas fermentation antibacterial not only needs suitable carbon-nitrogen ratio, in addition it is also necessary to some trace units Element and other trophic factors.Trace element cobalt (Co), nickel (Ni) can promote methanogen Growth and the activity of kinase, and then accelerate the biosynthesis of methane；Herba polygoni hydropiperis contains abundant Flavonoid and terpene substances, can remarkably promote the growth of numerous microorganism, from ancient times to the present make Wine yeast-making technology is widely used, but there is not yet above-mentioned technology contents and answer in terms of biogas fermentation Relevant report with research.
Summary of the invention
It is an object of the invention to for deficiency of the prior art, it is provided that a kind of raising methane gas producing Swine waste marsh gas leaven formula of amount and preparation method thereof,
For achieving the above object, the invention discloses following technical scheme:
A kind of swine waste marsh gas leaven formula, including following composition in parts by weight:
Agricultural crop straw: 15～25kg；
Biogas slurry: 1L；
Molasses: 200mL；
Testa oryzae: 1.5kg；
Fresh Herba polygoni hydropiperis: 2.5kg；
Clear water: 15L；
20% manganese sulfate solution: 20～40mL
The CoCl2 6H2O solution of 1mg/ml: 2～3；
2mg/ml NiCl2 6H2O solution: 2～3；
Microbial bacterial agent: 40～80g.
As a kind of preferred version, described microbial bacterial agent is bacillus cereus, lactic acid bacteria, biogas Zymogenous bacteria, cellulose hydrolysis bacterium.
The invention also discloses the preparation method of a kind of swine waste marsh gas leaven, including walking as follows Rapid:
S1 crop straw pre-treatment:
S1.1, in terms of every cube of effective fermentation volume of methane-generating pit, takes the crops of 15～25 parts Straw, cuts into 5～8cm length；
S1.2 adds saturated limewater in straw, and room temperature 10～35 DEG C soak 3-5 days, Obtain the straw of alkalization；
S2 prepares amplification culture base:
S2.1, in terms of every cube of effective fermentation volume of methane-generating pit, takes biogas slurry, molasses the most mixed After even, add Testa oryzae and cut into the fresh Herba polygoni hydropiperis of 5～8cm length, finally adding clear water, It is uniformly mixed so as to obtain cultivation basis thing；
S2.2 adds 20% manganese sulfate solution, CoCl in the thing of cultivation basis2·6H2O solution, NiCl2·6H2O solution, is uniformly mixed so as to obtain amplification culture base；
S3 adds microbial bacterial agent:
According to biogas fermentative liquid stereometer, add microbial bacterial agent, the usage amount of microbial bacterial agent It is 40～80g/m3；Microbial bacterial agent comprise bacillus cereus, lactic acid bacteria, mycete, yeast, At least four in biogas fermentation flora, cellulose hydrolysis bacterium, Nitromonas, effective bacterial content >= 1010cfu/g。
Further, in described step S2.2, fresh Herba polygoni hydropiperis is cut into the length of 4～6cm.
Further, in described step S2.2, in 20% manganese sulfate solution, manganese sulfate: go Ionized water=2:8.
Further, in described step S2.2, CoCl2·6H2O solution manufacturing method: take 0.1gCoCl2·6H2O is dissolved in 100mL deionized water, shakes up, and preserves in sealing in container.
Further, in described step S2.2, NiCl2·6H2O solution manufacturing method: take 0.2gNiCl2·6H2O is dissolved in 100mL deionized water, shakes up, and preserves in sealing in container.
Further, in described step S2.2, required manganese sulfate is technical grade； CoCl2·6H2O、NiCl2·6H2O is analytical pure specification.
Further, in described step S3, microbial bacterial agent be bacillus cereus, lactic acid bacteria, Biogas fermentation antibacterial, cellulose hydrolysis bacterium, mass ratio is followed successively by: 1～2:0.5～2:1～ 2:0.5～1.
A kind of swine waste marsh gas leaven formula disclosed by the invention and preparation method thereof, can be in natural pond Add this leaven formula in gas, make the carbon nitrogen source of fermentation system and trace element reach methane-generating pit The Appropriate of interior microorganism species growth, the growth of the biogas fermentation antibacterial in promotion methane-generating pit, Make marsh gas fermenting system stable, by adding inoculating microbe synergic fermentation, improve micro-life The thing utilization rate to raw material, reaches to improve the effect of methane gas producing amount.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.
A kind of swine waste marsh gas leaven formula, including following composition in parts by weight:
Agricultural crop straw: 15～25kg；
Biogas slurry: 1L；
Molasses: 200mL；
Testa oryzae: 1.5kg；
Fresh Herba polygoni hydropiperis: 2.5kg；
Clear water: 15L；
20% manganese sulfate solution: 20～40mL
The CoCl2 6H2O solution of 1mg/ml: 2～3；
2mg/ml NiCl2 6H2O solution: 2～3；
Microbial bacterial agent: 40～80g.Preferably bacillus cereus, lactic acid bacteria, biogas fermentation antibacterial, Cellulose hydrolysis bacterium, mass ratio is followed successively by 1～2:0.5～2:1～2:0.5～1.
The preparation method of a kind of swine waste marsh gas leaven, comprises the steps:
S1 crop straw pre-treatment:
S1.1, in terms of every cube of effective fermentation volume of methane-generating pit, takes the crops of 15～25 parts Straw, cuts into 5～8cm length；
S1.2 adds saturated limewater in straw, and room temperature 10～35 DEG C soak 3-5 days, Obtain the straw of alkalization；
S2 prepares amplification culture base:
S2.1, in terms of every cube of effective fermentation volume of methane-generating pit, takes biogas slurry, molasses the most mixed After even, add Testa oryzae and the fresh Herba polygoni hydropiperis cutting into 5～8cm length (cut by the freshest Herba polygoni hydropiperis Become the length of 4～6cm), finally add clear water, be uniformly mixed so as to obtain cultivation basis thing；
S2.2 adds 20% manganese sulfate solution (manganese sulfate: deionized water in the thing of cultivation basis =2:8), CoCl2·6H2O solution, NiCl2·6H2O solution, is uniformly mixed so as to obtain amplification culture base；
S3 adds microbial bacterial agent:
According to biogas fermentative liquid stereometer, add microbial bacterial agent, the usage amount of microbial bacterial agent It is 40～80g/m3；Microbial bacterial agent comprise bacillus cereus, lactic acid bacteria, mycete, yeast, At least four in biogas fermentation flora, cellulose hydrolysis bacterium, Nitromonas, effective bacterial content >= 1010cfu/g。
Preferably, in described step S2.2, CoCl2·6H2O solution manufacturing method: take 0.1gCoCl2·6H2O is dissolved in 100mL deionized water, shakes up, and preserves in sealing in container.
Preferably, in described step S2.2, NiCl2·6H2O solution manufacturing method: take 0.2gNiCl2·6H2O is dissolved in 100mL deionized water, shakes up, and preserves in sealing in container.
Preferably, in described step S2.2, required manganese sulfate is technical grade；CoCl2·6H2O、 NiCl2·6H2O is analytical pure specification.
Preferably, in described step S3, microbial bacterial agent is bacillus cereus, lactic acid bacteria, natural pond Gas zymogenous bacteria, cellulose hydrolysis bacterium, mass ratio is followed successively by: 1～2:0.5～2:1～2: 0.5～1.
Embodiment 1
A kind of swine waste marsh gas leaven formula, including following composition in parts by weight:
Agricultural crop straw: 15kg；
Biogas slurry: 1L；
Molasses: 200mL；
Testa oryzae: 1.5kg；
Fresh Herba polygoni hydropiperis: 2.5kg；
Clear water: 15L；
20% manganese sulfate solution: 20mL
The CoCl2 6H2O solution of 1mg/ml: 2；
2mg/ml NiCl2 6H2O solution: 2；
Microbial bacterial agent: 40g.Preferably bacillus cereus, lactic acid bacteria, biogas fermentation antibacterial, fibre Dimension element hydrolysis bacterium, mass ratio is followed successively by 1:0.5:1:0.5.
The preparation method of a kind of swine waste marsh gas leaven, comprises the steps:
S1 crop straw pre-treatment:
S1.1, in terms of every cube of effective fermentation volume of methane-generating pit, takes the agricultural crop straw of 15 parts, Cut into 5～8cm length；
S1.2 adds saturated limewater in straw, and room temperature 10～35 DEG C soak 3 days, Straw to alkalization；
S2 prepares amplification culture base:
S2.1, in terms of every cube of effective fermentation volume of methane-generating pit, takes biogas slurry, molasses the most mixed After even, add Testa oryzae and cut into the fresh Herba polygoni hydropiperis of 5～8cm length, finally adding clear water, It is uniformly mixed so as to obtain cultivation basis thing；
S2.2 adds 20% manganese sulfate solution (manganese sulfate: deionization in the thing of cultivation basis Water=2:8), CoCl2·6H2O solution, NiCl2·6H2O solution, is uniformly mixed so as to obtain amplification culture Base；
S3 adds microbial bacterial agent:
According to biogas fermentative liquid stereometer, add microbial bacterial agent, the usage amount of microbial bacterial agent For 40g/m3；Microbial bacterial agent comprises bacillus cereus, lactic acid bacteria, mycete, yeast, natural pond At least four in gas fermentative microflora, cellulose hydrolysis bacterium, Nitromonas, effective bacterial content >= 1010cfu/g。
In step S2.2, CoCl2·6H2O solution manufacturing method: take 0.1gCoCl2·6H2O It is dissolved in 100mL deionized water, shakes up, preserve in sealing in container.
In step S2.2, NiCl2·6H2O solution manufacturing method: take 0.2gNiCl2·6H2O It is dissolved in 100mL deionized water, shakes up, preserve in sealing in container.
In step S2.2, required manganese sulfate is technical grade；CoCl2·6H2O、NiCl2·6H2O It is analytical pure specification.
In step S3, microbial bacterial agent be bacillus cereus, lactic acid bacteria, biogas fermentation antibacterial, Cellulose hydrolysis bacterium, mass ratio is followed successively by: 1:0.5:1:0.5.
Embodiment 2
A kind of swine waste marsh gas leaven formula, including following composition in parts by weight:
Agricultural crop straw: 25kg；
Biogas slurry: 1L；
Molasses: 200mL；
Testa oryzae: 1.5kg；
Fresh Herba polygoni hydropiperis: 2.5kg；
Clear water: 15L；
20% manganese sulfate solution: 40mL
The CoCl2 6H2O solution of 1mg/ml: 3；
2mg/ml NiCl2 6H2O solution: 3；
Microbial bacterial agent: 80g.Preferably bacillus cereus, lactic acid bacteria, biogas fermentation antibacterial, fibre Dimension element hydrolysis bacterium, mass ratio is followed successively by 2:2:2:1.
The preparation method of a kind of swine waste marsh gas leaven, comprises the steps:
S1 crop straw pre-treatment:
S1.1, in terms of every cube of effective fermentation volume of methane-generating pit, takes the agricultural crop straw of 25 parts, Cut into 5～8cm length；
S1.2 adds saturated limewater in straw, and room temperature 10～35 DEG C soak 5 days, Straw to alkalization；
S2 prepares amplification culture base:
S2.1, in terms of every cube of effective fermentation volume of methane-generating pit, takes biogas slurry, molasses the most mixed After even, add Testa oryzae and cut into the fresh Herba polygoni hydropiperis of 4～6cm length, finally adding clear water, It is uniformly mixed so as to obtain cultivation basis thing；
S2.2 adds 20% manganese sulfate solution (manganese sulfate: deionization in the thing of cultivation basis Water=2:8), CoCl2·6H2O solution, NiCl2·6H2O solution, is uniformly mixed so as to obtain amplification culture Base；
S3 adds microbial bacterial agent:
According to biogas fermentative liquid stereometer, add microbial bacterial agent, the usage amount of microbial bacterial agent For 80g/m3；Microbial bacterial agent comprises bacillus cereus, lactic acid bacteria, mycete, yeast, natural pond At least four in gas fermentative microflora, cellulose hydrolysis bacterium, Nitromonas, effective bacterial content >= 1010cfu/g。
In step S2.2, CoCl2·6H2O solution manufacturing method: take 0.1gCoCl2·6H2O It is dissolved in 100mL deionized water, shakes up, preserve in sealing in container.
In step S2.2, NiCl2·6H2O solution manufacturing method: take 0.2gNiCl2·6H2O It is dissolved in 100mL deionized water, shakes up, preserve in sealing in container.
In step S2.2, required manganese sulfate is technical grade；CoCl2·6H2O、NiCl2·6H2O It is analytical pure specification.
In step S3, microbial bacterial agent be bacillus cereus, lactic acid bacteria, biogas fermentation antibacterial, Cellulose hydrolysis bacterium, mass ratio is followed successively by: 2:2:2:1.
Embodiment 3
A kind of swine waste marsh gas leaven formula, including following composition in parts by weight:
Agricultural crop straw: 20kg；
Biogas slurry: 1L；
Molasses: 200mL；
Testa oryzae: 1.5kg；
Fresh Herba polygoni hydropiperis: 2.5kg；
Clear water: 15L；
20% manganese sulfate solution: 30mL
The CoCl2 6H2O solution of 1mg/ml: 3；
2mg/ml NiCl2 6H2O solution: 3；
Microbial bacterial agent: 50g.Preferably bacillus cereus, lactic acid bacteria, biogas fermentation antibacterial, fibre Dimension element hydrolysis bacterium, mass ratio is followed successively by 2:1:1:1.
The preparation method of a kind of swine waste marsh gas leaven, comprises the steps:
S1 crop straw pre-treatment:
S1.1, in terms of every cube of effective fermentation volume of methane-generating pit, takes the agricultural crop straw of 20 parts, Cut into 5～8cm length；
S1.2 adds saturated limewater in straw, and room temperature 10～35 DEG C soak 4 days, Straw to alkalization；
S2 prepares amplification culture base:
S2.1, in terms of every cube of effective fermentation volume of methane-generating pit, takes biogas slurry, molasses the most mixed After even, add Testa oryzae and cut into the fresh Herba polygoni hydropiperis of 4～6cm length, finally adding clear water, It is uniformly mixed so as to obtain cultivation basis thing；
S2.2 adds 20% manganese sulfate solution (manganese sulfate: deionization in the thing of cultivation basis Water=2:8), CoCl2·6H2O solution, NiCl2·6H2O solution, is uniformly mixed so as to obtain amplification culture Base；
S3 adds microbial bacterial agent:
According to biogas fermentative liquid stereometer, add microbial bacterial agent, the usage amount of microbial bacterial agent For 50g/m3；Microbial bacterial agent comprises bacillus cereus, lactic acid bacteria, mycete, yeast, natural pond At least four in gas fermentative microflora, cellulose hydrolysis bacterium, Nitromonas, effective bacterial content >= 1010cfu/g。
In step S2.2, CoCl2·6H2O solution manufacturing method: take 0.1gCoCl2·6H2O It is dissolved in 100mL deionized water, shakes up, preserve in sealing in container.
In step S2.2, NiCl2·6H2O solution manufacturing method: take 0.2gNiCl2·6H2O It is dissolved in 100mL deionized water, shakes up, preserve in sealing in container.
In step S2.2, required manganese sulfate is technical grade；CoCl2·6H2O、NiCl2·6H2O It is analytical pure specification.
In step S3, microbial bacterial agent be bacillus cereus, lactic acid bacteria, biogas fermentation antibacterial, Cellulose hydrolysis bacterium, mass ratio is followed successively by: 2:1:1:1.
Microbial bacterial agent in the present invention also can replace with EM biogas fermentation microbial inoculum, each company Although the ratio of various bacterium is different in the EM biogas fermentation bacterium produced, but for biogas fermentation The content of main bacteria seed is more or less the same, and all can reach the ferment effect of the present invention.
The using method of a kind of swine waste marsh gas leaven disclosed by the invention is as follows:
Microbial bacterial agent, mixing, room temperature (10～35 DEG C) is added in the amplification culture base obtained Cultivating 3～5 days, midway every stirring in 12 hours once, obtains microorganism mixed culture； The agricultural crop straw of the microorganism mixed culture obtained with alkalization is put into and adds by normal rates Enter water and carry out biogas fermentation in the methane-generating pit of pig manure.Wherein, alkalization agricultural crop straw every 7 days with once；Microorganism mixed culture, under the environment of temperature on average >=25 DEG C, is used the bimester of per Once, under 25 DEG C of environment of temperature on average ＜, monthly with once.
The carbon-nitrogen ratio of a kind of swine waste marsh gas leaven scalable fermentation raw material disclosed by the invention, makes Fermentation raw material carbon-nitrogen ratio reaches the Appropriate needed for biogas fermentation antibacterial, by microorganism mixing training Support the microorganism synergic fermentation in thing and in methane-generating pit, bacillus cereus, lactic acid bacteria, mycete, ferment At least four microorganism in female bacterium, biogas fermentation antibacterial, cellulose hydrolysis bacterium, Nitromonas is filled Divide and utilize fermentation raw material；Traditional marsh gas fermentation pool raw material availability is about 50%, passes through Using inventive formulation, raw material availability can be made to bring up to 55～65%, fermentation raw material utilizes Rate improves 5～15% compared to traditional biogas fermentation material utilization rate；
Traditional marsh gas fermentation pool biogas fermentation is unstable, and in pond, growth of microorganism is unstable, shadow Ring production rate of methane.Fermentation vat every cube of gas production every day traditional under room temperature (10～35 DEG C) It is about 0.22m3；The application of the invention formula, adds trace element cobalt (Co), nickel (Ni) Stimulating biogas fermentation antibacterial, promote its Fast-propagation, in making marsh gas fermentation pool, microorganism species is steady Fixed, thus maintain marsh gas fermenting system operation stability；By improving raw material availability and maintenance The stability of marsh gas fermenting system, marsh gas fermentation pool every cube of gas production every day can reach 0.24～ 0.26m3, improve 9～18% compared to traditional marsh gas fermentation pool gas production.
The above is only the preferred embodiment of the present invention, is not intended to limit；It should be pointed out that, Although the present invention being described in detail with reference to the various embodiments described above, the ordinary skill of this area Personnel should be appreciated that the technical scheme described in the various embodiments described above still can be repaiied by it Change, or the most some or all of technical characteristic is carried out equivalent；And these are revised and replace Change, do not make the essence of corresponding technical scheme depart from the model of various embodiments of the present invention technical scheme Enclose.

Claims (9)

1. a swine waste marsh gas leaven formula, it is characterised in that include following with weight portion The composition of meter:
Agricultural crop straw: 15～25kg；
Biogas slurry: 1L；
Molasses: 200mL；
Testa oryzae: 1.5kg；
Fresh Herba polygoni hydropiperis: 2.5kg；
Clear water: 15L；
20% manganese sulfate solution: 20～40mL
The CoCl2 6H2O solution of 1mg/ml: 2～3；
2mg/ml NiCl2 6H2O solution: 2～3；
Microbial bacterial agent: 40～80g.
A kind of swine waste marsh gas leaven formula the most according to claim 1, its feature exists In, described microbial bacterial agent is bacillus cereus, lactic acid bacteria, biogas fermentation antibacterial, cellulose water Solve bacterium.
3. the preparation method of a swine waste marsh gas leaven, it is characterised in that comprise the steps:
S1 crop straw pre-treatment:
S1.1, in terms of every cube of effective fermentation volume of methane-generating pit, takes the crops of 15～25 parts Straw, cuts into 5～8cm length；
S1.2 adds saturated limewater in straw, and room temperature 10～35 DEG C soak 3-5 days, Obtain the straw of alkalization；
S2 prepares amplification culture base:
S2.1, in terms of every cube of effective fermentation volume of methane-generating pit, takes biogas slurry, molasses the most mixed After even, add Testa oryzae and cut into the fresh Herba polygoni hydropiperis of 5～8cm length, finally adding clear water, It is uniformly mixed so as to obtain cultivation basis thing；
S2.2 adds 20% manganese sulfate solution, CoCl in the thing of cultivation basis2·6H2O solution, NiCl2·6H2O solution, is uniformly mixed so as to obtain amplification culture base；
S3 adds microbial bacterial agent:
According to biogas fermentative liquid stereometer, add microbial bacterial agent, the usage amount of microbial bacterial agent It is 40～80g/m3；Microbial bacterial agent comprise bacillus cereus, lactic acid bacteria, mycete, yeast, At least four in biogas fermentation flora, cellulose hydrolysis bacterium, Nitromonas, effective bacterial content >= 1010cfu/g。
A kind of preparation method of swine waste marsh gas leaven, its feature Being, in described step S2.2, fresh Herba polygoni hydropiperis is cut into the length of 4～6cm.
A kind of preparation method of swine waste marsh gas leaven, its feature It is, in described step S2.2, in 20% manganese sulfate solution, manganese sulfate: deionized water=2:8.
A kind of preparation method of swine waste marsh gas leaven, its feature It is, in described step S2.2, CoCl2·6H2O solution manufacturing method: take 0.1gCoCl2·6H2O It is dissolved in 100mL deionized water, shakes up, preserve in sealing in container.
A kind of preparation method of swine waste marsh gas leaven, its feature It is, in described step S2.2, NiCl2·6H2O solution manufacturing method: take 0.2gNiCl2·6H2O It is dissolved in 100mL deionized water, shakes up, preserve in sealing in container.
A kind of preparation method of swine waste marsh gas leaven, its feature Being, in described step S2.2, required manganese sulfate is technical grade；CoCl2·6H2O、 NiCl2·6H2O is analytical pure specification.
The preparation method of a kind of swine waste marsh gas leaven the most according to claim 3, it is special Levying and be, in described step S3, microbial bacterial agent is that bacillus cereus, lactic acid bacteria, biogas are sent out Ferment antibacterial, cellulose hydrolysis bacterium, mass ratio is followed successively by: 1～2:0.5～2:1～2:0.5～ 1。
CN201610248472.8A 2016-04-20 2016-04-20 Pig manure methane fermenting agent formula and preparation method thereof Pending CN105861367A (en)

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CN107056001A (en) * 2017-02-28 2017-08-18 铜仁市万山区绿涛牧业有限公司 A kind of method that pig manure produces biogas
CN107445407A (en) * 2017-09-08 2017-12-08 常州思宇环保材料科技有限公司 A kind of waste water treatment system for nursery and its processing method
CN107674907A (en) * 2017-11-30 2018-02-09 江苏中科重工股份有限公司 A kind of zymotic fluid for biogas fermentation
CN107858301A (en) * 2017-10-28 2018-03-30 丁忠良 A kind of complex micro organism fungicide formula and preparation method thereof

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CN107056001A (en) * 2017-02-28 2017-08-18 铜仁市万山区绿涛牧业有限公司 A kind of method that pig manure produces biogas
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CN107445407A (en) * 2017-09-08 2017-12-08 常州思宇环保材料科技有限公司 A kind of waste water treatment system for nursery and its processing method
CN107858301A (en) * 2017-10-28 2018-03-30 丁忠良 A kind of complex micro organism fungicide formula and preparation method thereof
CN107674907A (en) * 2017-11-30 2018-02-09 江苏中科重工股份有限公司 A kind of zymotic fluid for biogas fermentation

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