CN107056001B - Method for producing biogas from pig manure - Google Patents
Method for producing biogas from pig manure Download PDFInfo
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- CN107056001B CN107056001B CN201710113726.XA CN201710113726A CN107056001B CN 107056001 B CN107056001 B CN 107056001B CN 201710113726 A CN201710113726 A CN 201710113726A CN 107056001 B CN107056001 B CN 107056001B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The invention belongs to the field of fermentation technology, and particularly relates to a method for producing biogas by using pig manure.
Description
Technical Field
The invention belongs to the field of fermentation technology, and particularly relates to a method for producing biogas from pig manure.
Background
The biogas fermentation is a microbial process, which is mainly divided into 3 stages of liquefaction, acid production and methane production, wherein in the liquefaction stage, crop straws, human and animal excreta, garbage and other various organic wastes, usually carbohydrates existing in a macromolecular state, are subjected to enzymolysis through extracellular enzymes secreted by microbes, and are decomposed into water-soluble micromolecule compounds, namely polysaccharide is hydrolyzed into monosaccharide or disaccharide, protein is hydrolyzed into peptide and amino acid, and fat is decomposed into glycerol and fatty acid, so that the micromolecule compounds can enter cells to perform a series of biochemical reactions; the acid production stage is to convert monosaccharides, peptides, amino acids, glycerol, fatty acids and other substances into simple organic acids, alcohols, carbon dioxide, hydrogen, ammonia, hydrogen sulfide and the like under the action of different methane microbial populations, and the main products are volatile organic acids; in the methane production stage, substances such as organic acid, alcohol, carbon dioxide and ammonia are decomposed into mixed gas mainly containing methane and carbon dioxide by methane bacteria, namely methane.
The anaerobic digestion process of biogas fermentation is divided into three stages, wherein the first stage is a hydrolysis stage, and macromolecular complex organic matters are degraded into micromolecular soluble organic matters such as low-molecular carbohydrate compounds, fatty acid, amino acid and the like under the action of hydrolase secreted by hydrolytic bacteria; the second stage is a hydrogen-producing acetic acid stage, and the low molecular weight carbohydrate is further degraded into acetic acid under the action of hydrogen-producing acetogenic flora, and hydrogen and carbon dioxide are generated; the third stage is a methane production stage, and methane is produced by methanogens using hydrogen, carbon dioxide and acetic acid; most fermentation raw materials are generally produced into complex macromolecular organic matters, and the problems of low reaction efficiency, insufficient reaction, low methane yield and the like can occur in the first stage of the fermentation process.
Disclosure of Invention
The invention is realized by the following technical scheme:
in order to solve the problems, the invention provides a raw material for producing biogas, which consists of pig manure, ficus microcarpa leaves, amylase, lignin degrading bacteria, reaction auxiliary agents, bentonite and active carbon.
The raw materials comprise, by weight, 40-70 parts of pig manure, 1-10 parts of ficus microcarpa leaves, 0.01-0.05 part of lignin degrading bacteria, 0.1-0.5 part of amylase, 0.5-1.5 parts of reaction auxiliaries, 5-8 parts of bentonite and 1-3 parts of activated carbon.
The reaction auxiliary agent is at least one of potassium chloride, D-sorbitol, glycerol and trehalose.
The concentration of the potassium chloride is 0.5-2 mmol/L, the concentration of the D-sorbitol is 150-300 mmol/L, the concentration of the glycerol is 20-80 mmol/L, and the concentration of the trehalose is 150-300 mmol/L.
The lignin-degrading bacteria are at least one of Phanerochaete chrysosporium and Pleurotus eryngii.
The invention also aims to provide a method for producing biogas from pig manure, which comprises the following steps:
(1) mixing: drying and crushing banyan leaves, sieving the crushed banyan leaves with a 100-200-mesh sieve, and mixing banyan leaf powder and pig manure according to the weight ratio of 1: (4.2-5.8) mixing and stirring at a stirring speed of 40-60 r/min and a temperature of 55-60 ℃, stirring for 60-120 min, and adjusting the pH to 6.5-7.1 to obtain a fermentation material liquid;
(2) and (3) treatment: uniformly mixing the fermentation liquid and an amylase agent, and then putting the mixture into a constant temperature device with the temperature set to be 60-70 ℃ for treatment for 1-2 hours;
(3) fermentation: and (3) adding lignin degrading bacteria, bentonite and active carbon into the fermentation liquid treated in the step (2), mixing and stirring uniformly at the stirring speed of 60-80 r/min for 30-60 min, and sealing and fermenting the mixture in a fermentation tank.
The TS concentration of the fermentation liquid is adjusted to be 10% -15%.
The pig manure and the ficus microcarpa leaves are subjected to ultrasonic treatment before being mixed and stirred.
The ultrasonic treatment frequency is 40-50 KHz, the temperature is 40-60 ℃, and the treatment time is 10-20 min.
Has the advantages that:
the invention adopts the pig manure and the ficus microcarpa leaves as the fermentation raw materials, and the pig manure is contained in the pig manureThe organic matters are effectively degraded, the requirement of microbial growth in the early stage of fermentation is met, and meanwhile, the ratio of the pig manure to the ficus microcarpa leaves is 1: (4.2-5.8), after proportioning, the cellulose, hemicellulose and other components in the ficus microcarpa leaves can be effectively utilized by microorganisms, lignin degrading bacteria can generate lignin degrading enzyme in the fermentation process, cellulose components in the fermentation raw materials are degraded, cellulose blocking the fermentation process is reduced, the addition of reaction auxiliary agents provides trace elements for the whole fermentation process, provides nutrition for methanogens, promotes the growth acceleration of methanogens, improves the activity change of biological strains in a fermentation tank, further improves the degradation rate of organic matters, the bentonite has good adsorption performance, and Ca is released in the fermentation process+、Mg2+The ions can promote the anaerobic fermentation of the pig manure, the active carbon has a special porous structure, a large amount of organic matters in fermentation liquor and DO in water can be adsorbed, the condition for degrading the organic matters is provided for microorganisms on the surface of the active carbon, in addition, the adsorption effect of the active carbon can also improve the contact time of the organic matters and the microorganisms, and the decomposition rate of the organic matters is accelerated.
The method adopts pig manure, ficus microcarpa leaves, amylase, lignin degrading bacteria, reaction auxiliary agents, bentonite and active carbon as raw materials, and the organic matters in the raw materials are effectively degraded by the synergistic effect of the components after proportioning, so that the problems of low efficiency and insufficient reaction of macromolecular organic matters in the first stage of fermentation are solved, the methane yield is obviously improved, and the fermentation rate is accelerated.
Detailed Description
Example 1
The formula is as follows: 70kg of pig manure, 14kg of ficus microcarpa leaves, 0.05kg of lignin degrading bacteria, 0.4kg of amylase, 0.5kg of reaction auxiliary agent, 5kg of bentonite and 1kg of active carbon;
the preparation method comprises the following steps:
(1) mixing: drying and crushing banyan leaves, sieving with a 100-mesh sieve, mixing the banyan leaf powder and pig manure according to the weight ratio of 1: 5, mixing and stirring at the stirring speed of 40r/min and the temperature of 55-60 ℃, stirring for 60min, and adjusting the pH to 6.5 to obtain a fermentation material liquid;
(2) and (3) treatment: uniformly mixing the fermentation liquid and an amylase agent, and then putting the mixture into a constant temperature device with the temperature set to be 60-65 ℃ for processing for 1 hour;
(3) fermentation: and (3) adding lignin degrading bacteria, bentonite and active carbon into the fermentation liquid treated in the step (2), mixing and stirring uniformly at the stirring speed of 80r/min for 50min, and sealing and fermenting the mixture in a fermentation tank.
The reaction auxiliary agent is potassium chloride.
The concentration of the potassium chloride is 0.5 mmol/L.
The lignin degrading bacteria are Phanerochaete chrysosporium.
And regulating the TS concentration of the fermentation liquid to be 10%.
The pig manure and the ficus microcarpa leaves are subjected to ultrasonic treatment before being mixed and stirred.
The ultrasonic treatment frequency is 40KHz, the temperature is 40-60 ℃, and the treatment time is 10 min.
Example 2
The formula is as follows: 40kg of pig manure, 10kg of ficus microcarpa leaves, 0.01kg of lignin degrading bacteria, 0.2kg of amylase, 0.5kg of reaction auxiliary agent, 5kg of bentonite and active carbon;
the preparation method comprises the following steps:
(1) mixing: drying and crushing banyan leaves, sieving with a 200-mesh sieve, mixing the banyan leaf powder and pig manure according to the weight ratio of 1: mixing and stirring at a ratio of 4, wherein the stirring speed is 40r/min, the temperature is 55-60 ℃, stirring for 100min, and adjusting the pH to 7.1 to obtain a fermentation material liquid;
(2) and (3) treatment: uniformly mixing the fermentation liquid and an amylase agent, and then putting the mixture into a constant temperature device with the temperature set to 65-70 ℃ for processing for 1.5 h;
(3) fermentation: and (3) adding lignin degrading bacteria, bentonite and active carbon into the fermentation liquid treated in the step (2), mixing and stirring uniformly at the stirring speed of 80r/min for 60min, and sealing and fermenting the mixture in a fermentation tank.
The reaction auxiliary agent is D-sorbitol.
The concentration of the D-sorbitol is 300 mmol/L.
The lignin-degrading bacteria are Pleurotus ostreatus.
And regulating the TS concentration of the fermentation liquid to be 15%.
The pig manure and the ficus microcarpa leaves are subjected to ultrasonic treatment before being mixed and stirred.
The ultrasonic treatment frequency is 50KHz, the temperature is 40-60 ℃, and the treatment time is 10 min.
Example 3
The formula is as follows: 60kg of pig manure, 10kg of ficus microcarpa leaves, 0.03kg of lignin degrading bacteria, 0.3kg of amylase, 1kg of reaction auxiliary agent, 7kg of bentonite and 3kg of active carbon;
the preparation method comprises the following steps:
(1) mixing: drying and crushing banyan leaves, sieving with a 180-mesh sieve, mixing banyan leaf powder and pig manure according to the weight ratio of 1: mixing and stirring at a ratio of 6, wherein the stirring speed is 50r/min, the temperature is 55-60 ℃, stirring for 100min, and adjusting the pH to 6.8 to obtain a fermentation feed liquid;
(2) and (3) treatment: uniformly mixing the fermentation liquid and an amylase agent, and then putting the mixture into a constant temperature device with the temperature set to 65-70 ℃ for treatment for 2 hours;
(3) fermentation: and (3) adding lignin degrading bacteria, bentonite and active carbon into the fermentation liquid treated in the step (2), mixing and stirring uniformly at the stirring speed of 70r/min for 50min, and sealing and fermenting the mixture in a fermentation tank.
The reaction auxiliary agent is glycerol.
The concentration of the glycerol is 60 mmol/L.
The lignin-degrading bacteria are a combination of Phanerochaete chrysosporium and Pleurotus eryngii.
The weight ratio of the phanerochaete chrysosporium to the pleurotus eryngii is 1: 1.
and the TS concentration of the fermentation liquid is adjusted to be 13%.
The pig manure and the ficus microcarpa leaves are subjected to ultrasonic treatment before being mixed and stirred.
The ultrasonic treatment frequency is 45KHz, the temperature is 50 ℃, and the treatment time is 15 min.
Example 4
The formula is as follows: 50kg of pig manure, 10kg of ficus microcarpa leaves, 0.04kg of lignin degrading bacteria, 0.4kg of amylase, 1.5kg of reaction auxiliary agent, 6kg of bentonite and 1kg of active carbon;
the preparation method comprises the following steps:
(1) mixing: drying and crushing banyan leaves, sieving with a 160-mesh sieve, mixing the banyan leaf powder and pig manure according to the weight ratio of 1: 5, mixing and stirring at the stirring speed of 60r/min and the temperature of 55-60 ℃, stirring for 90min, and adjusting the pH to 6.7 to obtain a fermentation feed liquid;
(2) and (3) treatment: uniformly mixing the fermentation liquid and an amylase agent, and then putting the mixture into a constant temperature device with the temperature set to be 60-65 ℃ for processing for 1 hour;
(3) fermentation: and (3) adding lignin degrading bacteria, bentonite and active carbon into the fermentation liquid treated in the step (2), mixing and stirring uniformly at the stirring speed of 80r/min for 45min, and sealing and fermenting the mixture in a fermentation tank.
The reaction auxiliary agent is trehalose.
The trehalose concentration is 250 mmol/L.
The lignin-degrading bacteria are Pleurotus ostreatus.
And the TS concentration of the fermentation liquid is adjusted to be 14%.
The pig manure and the ficus microcarpa leaves are subjected to ultrasonic treatment before being mixed and stirred.
The ultrasonic treatment frequency is 50KHz, the temperature is 60 ℃, and the treatment time is 20 min.
Example 5
The formula is as follows: 60kg of pig manure, 10kg of ficus microcarpa leaves, 0.04kg of lignin degrading bacteria, 0.3kg of amylase, 0.8kg of reaction auxiliary agent, 7kg of bentonite and 2.5kg of active carbon;
the preparation method comprises the following steps:
(1) mixing: drying and crushing banyan leaves, sieving with a 200-mesh sieve, mixing the banyan leaf powder and pig manure according to the weight ratio of 1: mixing and stirring at a ratio of 6, wherein the stirring speed is 60r/min, the temperature is 55-60 ℃, stirring for 100min, and adjusting the pH to 7.0 to obtain a fermentation feed liquid;
(2) and (3) treatment: uniformly mixing the fermentation liquid and an amylase agent, and then putting the mixture into a constant temperature device with the temperature set to be 60-65 ℃ for treatment for 2 hours;
(3) fermentation: and (3) adding lignin degrading bacteria, bentonite and active carbon into the fermentation liquid treated in the step (2), mixing and stirring uniformly at the stirring speed of 70r/min for 40min, and sealing and fermenting the mixture in a fermentation tank.
The reaction auxiliary agent is D-sorbitol.
The concentration of the D-sorbitol is 300 mmol/L.
The weight ratio of the phanerochaete chrysosporium to the pleurotus eryngii is 1: 2.
and regulating the TS concentration of the fermentation liquid to be 11%.
The pig manure and the ficus microcarpa leaves are subjected to ultrasonic treatment before being mixed and stirred.
The ultrasonic treatment frequency is 40KHz, the temperature is 40 ℃, and the treatment time is 10 min.
Test example 1
The embodiment group adopts the method for producing the biogas; the control group adopts a traditional biogas fermentation method, and human manure, cow manure, chicken manure and air-dried corn stalks are used as raw materials for fermentation; the fermentation period is 7d, and the test results are as follows:
tests show that the gas yield obtained by the fermentation method is obviously higher than that obtained by the traditional fermentation.
Claims (9)
1. The method for producing the biogas from the pig manure is characterized in that the raw materials comprise, by weight, 40-70 parts of the pig manure, 10-20 parts of ficus microcarpa leaves, 0.01-0.05 part of lignin degrading bacteria, 0.2-0.4 part of amylase, 0.5-1.5 parts of reaction auxiliary agents, 5-8 parts of bentonite and 1-3 parts of activated carbon;
the preparation method of the biogas produced by the pig manure comprises the following steps:
(1) mixing: drying and crushing banyan leaves, sieving the crushed banyan leaves with a 100-200-mesh sieve, and mixing banyan leaf powder and pig manure according to the weight ratio of 1: (4-6) mixing and stirring in proportion, wherein the stirring speed is 40-60 r/min, the temperature is 55-60 ℃, stirring is carried out for 60-120 min, and the pH is adjusted to be 6.5-7.1 to obtain a fermentation material liquid;
(2) and (3) treatment: uniformly mixing the fermentation liquid and an amylase agent, and then putting the mixture into a constant temperature device with the temperature set to be 60-70 ℃ for treatment for 1-2 hours;
(3) fermentation: and (3) adding lignin degrading bacteria, bentonite and active carbon into the fermentation liquid treated in the step (2), mixing and stirring uniformly at the stirring speed of 60-80 r/min for 30-60 min, and placing the mixture into a fermentation tank for anaerobic fermentation.
2. The method for producing biogas according to claim 1, wherein the reaction auxiliary agent is at least one of potassium chloride, D-sorbitol, glycerol, and trehalose.
3. The method for producing biogas from pig manure according to claim 2, wherein the concentration of potassium chloride is 0.5 to 2mmol/L, the concentration of D-sorbitol is 150 to 300mmol/L, the concentration of glycerol is 20 to 80mmol/L, and the concentration of trehalose is 150 to 300 mmol/L.
4. The method for producing biogas according to claim 1, wherein the amylase agent comprises at least one of alpha-amylase, beta-amylase, and gamma-amylase.
5. The method for producing biogas according to claim 1, wherein the reaction auxiliary agent is at least one of ferrous nitrate 1.5mg/L, nickel chloride hexahydrate 0.15mg/L, and cobalt chloride hexahydrate 0.3 mg/L.
6. The method for producing biogas according to claim 1, wherein the lignin-degrading bacteria is at least one of Phanerochaete chrysosporium and Pleurotus niveus.
7. The method for producing biogas from pig manure as claimed in claim 1, wherein the TS concentration of the fermentation liquor is adjusted to 10-15%.
8. The method for producing biogas according to claim 1, wherein the pig manure and ficus microcarpa leaves are subjected to ultrasonic treatment before mixing and stirring.
9. The method for producing biogas according to claim 8, wherein the ultrasonic treatment frequency is 40 to 50KHz, the temperature is 40 to 60 ℃, and the treatment time is 10 to 20 min.
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| CN111592402A (en) * | 2020-05-11 | 2020-08-28 | 福建省农业科学院畜牧兽医研究所 | Method for improving fermentation efficiency of pig manure |
| CN114196654A (en) * | 2021-12-17 | 2022-03-18 | 黑龙江省农业科学院农村能源与环保研究所 | Complex enzyme preparation, preparation method thereof and method for producing biogas |
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| JP2000254697A (en) * | 1999-03-12 | 2000-09-19 | Mitsubishi Heavy Ind Ltd | Treatment of organic waste |
| US20140147533A1 (en) * | 2012-11-27 | 2014-05-29 | Tuskegee University | Feed supplement products and methods of using such products for improved raising of ruminant livestock animals |
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| CN103626608A (en) * | 2013-11-06 | 2014-03-12 | 北京博文合众生物科技有限公司 | Technology and process for preparing biological humic acid fertilizer by use of pig manure |
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