CN106818543A - A kind of grass carp and Tilapia mossambica ecological polyculturing method - Google Patents
A kind of grass carp and Tilapia mossambica ecological polyculturing method Download PDFInfo
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- CN106818543A CN106818543A CN201510860032.3A CN201510860032A CN106818543A CN 106818543 A CN106818543 A CN 106818543A CN 201510860032 A CN201510860032 A CN 201510860032A CN 106818543 A CN106818543 A CN 106818543A
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- 241000276701 Oreochromis mossambicus Species 0.000 title claims abstract description 22
- 241000252230 Ctenopharyngodon idella Species 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 17
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 17
- 239000001301 oxygen Substances 0.000 claims abstract description 17
- 239000006041 probiotic Substances 0.000 claims abstract description 12
- 235000018291 probiotics Nutrition 0.000 claims abstract description 12
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 4
- 241000272525 Anas platyrhynchos Species 0.000 claims abstract description 3
- 238000012258 culturing Methods 0.000 claims abstract description 3
- 210000003608 fece Anatomy 0.000 claims abstract description 3
- 239000010871 livestock manure Substances 0.000 claims abstract description 3
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 12
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 4
- JJEJDZONIFQNHG-UHFFFAOYSA-N [C+4].N Chemical compound [C+4].N JJEJDZONIFQNHG-UHFFFAOYSA-N 0.000 claims description 4
- 239000011575 calcium Substances 0.000 claims description 4
- 229910052791 calcium Inorganic materials 0.000 claims description 4
- MMCOUVMKNAHQOY-UHFFFAOYSA-N carbonoperoxoic acid Chemical compound OOC(O)=O MMCOUVMKNAHQOY-UHFFFAOYSA-N 0.000 claims description 4
- 238000000855 fermentation Methods 0.000 claims description 2
- 230000004151 fermentation Effects 0.000 claims description 2
- GOLXNESZZPUPJE-UHFFFAOYSA-N spiromesifen Chemical compound CC1=CC(C)=CC(C)=C1C(C(O1)=O)=C(OC(=O)CC(C)(C)C)C11CCCC1 GOLXNESZZPUPJE-UHFFFAOYSA-N 0.000 claims 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 abstract description 18
- 241000251468 Actinopterygii Species 0.000 abstract description 8
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 7
- 239000003814 drug Substances 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 6
- 230000036039 immunity Effects 0.000 abstract description 3
- 229940079593 drug Drugs 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 19
- 241000894006 Bacteria Species 0.000 description 10
- 238000005286 illumination Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
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- 230000015556 catabolic process Effects 0.000 description 3
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- 238000006731 degradation reaction Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 108020004465 16S ribosomal RNA Proteins 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 240000003826 Eichhornia crassipes Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 244000207740 Lemna minor Species 0.000 description 2
- 235000006439 Lemna minor Nutrition 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 235000001855 Portulaca oleracea Nutrition 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
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- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000252228 Ctenopharyngodon Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 206010020741 Hyperpyrexia Diseases 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 239000005662 Paraffin oil Substances 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
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- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
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- 238000002955 isolation Methods 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 1
- 229940074404 sodium succinate Drugs 0.000 description 1
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- 238000004659 sterilization and disinfection Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of grass carp and Tilapia mossambica ecological polyculturing method, it comprises the following steps：1) culturing pool selection：Selection depth more than 2m, area is 23 mu of pond, and 1/4 1/3 area is separated with filter, in high temperature season, when water temperature is more than more than 30 DEG C, forbids duck excrement, pig manure water to enter the pool；2) pasture and water are planted：The Zhong Zhi great Piao in the pond that filter separates, it is 60 80/square meter to control great Piao density；Control pond is partly dissolved oxygen in more than 3mg/L without pasture and water, and the maximum temperature in the middle of a day controls at pasture and water temperature below 32 DEG C；3) in high temperature season per first quarter moon with an aquatic products probiotics.Present invention determine that grass carp, Tilapia mossambica do not like the pasture and water " big Piao " for ingesting; be used in combination the probiotics of drop nitrite; when pasture and water cover pond 1/4; 23 DEG C of temperature in water can be reduced; there is good adjustment effect to ammonia nitrogen, nitrite in water; the immunity of fish can be significantly improved, greatly reduced because of the edible risk that medication brings.
Description
Technical field
The invention belongs to aquaculture field, and in particular to a kind of grass carp and Tilapia mossambica ecological polyculturing method.
Background technology
It is also one of larger region of yield that Changtai area is that whole Zhangzhou Area cultivates black Rofe and concentrate the most, and the black mu of Rofe cultured area more than 10,000 produces black 1.2 ten thousand tons of Rofe per year, and the Tilapia mossambica for largely breaking out recently " streptococcosis " is urgent problem.
Tilapia mossambica " streptococcus " disease has not had effective method to control always since being broken out from 2009, and being even more within nearly 2 years causes Rofe fish medicine residual exceeded, outlet obstructions, and domestic unsalable, fish valency is at a low tide, and fatefulue strike is brought to whole Tilapia mossambica industry.This year Tilapia mossambica streptococcosis it is popular than in other years drug resistance is remarkably reinforced more extensively, Tu Tang cultivates the black raiser of Rofe more than 80% and significantly limits the quantity, and feeding volume is only that between the 0.1-0.5% of body weight, slightly dosage will cause significantly dead.Therefore, it is badly in need of a kind of ecologic breeding Synthetical prevention method of health, streptococcic generation can be controlled, also can guarantee that the edible safety of Tilapia mossambica.
The content of the invention
In order to solve the above problems, the present invention provides a kind of grass carp and Tilapia mossambica ecological polyculturing method.
The grass carp and Tilapia mossambica ecological polyculturing method that the present invention is provided comprise the following steps：
1) culturing pool selection：Selection depth more than 2m, area is 2-3 mus of pond, and the area of 1/4-1/3 is separated with filter, in high temperature season, when water temperature is more than more than 30 DEG C, forbids duck excrement, pig manure water to enter the pool；
2) pasture and water are planted：The Zhong Zhi great Piao in the pond that filter separates, it is 60-80/square meter to control great Piao density；Control pond is partly dissolved oxygen in more than 3mg/L without pasture and water, and the maximum temperature in the middle of a day controls at pasture and water temperature below 32 DEG C；
3) in high temperature season per first quarter moon with an aquatic products probiotics.
Wherein, described aquatic products probiotics is formed by Rhodopseudomonas palustris Rhodopseudomonas palustris CGMCC No.9136 fermentations.
The separation qualification process of Rhodopseudomonas palustris (Rhodopseudomonaspalustris) of the invention is as follows：
1. photosynthetic bacteria culture medium is prepared
Enriched medium (/L)：NaHCO32g, dusty yeast 1g, K2HPO4·3H2O 0.2g, NH4Cl 1g, NaAc3H2O 0.3g, MgSO4·7H2O 0.2g, NaCl 0.2g, Na2S2O3·5H2O 1g, pH7.0,121 DEG C of autoclaving 20min.
Isolation medium adds agar on the basis of enriched medium, and final mass is divided into 1.5%, 121 DEG C of autoclaving 20min.
2. it is enriched with：
The muddy water sample for coming will be gathered to be put in the anaerobism pipe with screw socket added with enriched medium, wherein anaerobism pipe fills 15mL culture mediums and 10mL pond waters+bed mud, each covering 2-3cm paraffin oil.Intensity of illumination 1000lx~2000lx, 30 DEG C of Anaerobic culturels.
3. isolate and purify
After being enriched with one week, 1mL liquid is taken out in the anaerobism pipe reddened from culture medium, it is 10 to take dilution factor-3、10-4, 10-5The μ L coated plates of liquid 100, observed that red colonies grow within 10 days or so；Choose the setting-out of red colonies flat board to pure.
4. identify
Picking single bacterium colony carries out 16S rRNA bacterium colony PCR, send Invitrogen to be sequenced, and sequence is as shown in SEQ ID No.1.Measure sequence carries out Blast comparisons on NCBI, is initially identified as Rhodopseudomonas palustris Rhodopseudomonas palustris, is 100% with the 16S rRNA genes similarity distance of Rhodopseudomonas palustris R.palustrisATCC 17003.The bacteria growing is slow, may occur in which within 5 days single bacterium colony, size 0.5mm~1mm；It is kermesinus bacterium colony under anaerobic state, Gram's staining is negative rod-short, and solid plate culture is rod-short (Fig. 1).
Rhodopseudomonas palustris Rhodopseudomonas palustris will be separated to and be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, abbreviation CGMCC, address on May 8th, 2014：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, deposit number is：CGMCC No.9136.
In one embodiment of the invention, pond culture density is every mu of 300-500 tail of grass carp, every mu of 1500-2000 tail of Tilapia mossambica.
In one embodiment of the invention, the density of great Piao is controlled as follows：By carbon ammonium and percarbonic acid calcium by weight 5：1 ratio is mixed, and is locally splashed at pasture and water, and directly pulvis is sprinkling upon on pasture and water, every mu of 5-10kg.
Wherein, the filter sky Plastic bottle plug enters mesh bag and is obtained.
Present invention determine that grass carp, Tilapia mossambica do not like the pasture and water " big Piao " for ingesting; have selected the medicine that pasture and water are killed in control; be used in combination the probiotics of drop nitrite; when pasture and water cover pond 1/4; 2-3 DEG C of temperature in water can be reduced, there is good adjustment effect to ammonia nitrogen, nitrite in water, the immunity of fish can be significantly improved; effectively prevent the generation of hammer disease, reduce the edible risk that medicinal application brings.
Brief description of the drawings
Degradation effect of the photosynthetic bacteria to nitrite under the conditions of two kinds of Fig. 1.The left side is degradeds of the CGMCC No.9136 to nitrite under no light condition, and the right is degradeds of the CGMCC No.9136 to nitrite under illumination condition.
Fig. 2 show the experiment pool and control pool temperature changes with time.
Fig. 3 show the experiment pool and control pool dissolved oxygen changes with time.
Specific embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Embodiment 1
Find that local buoyancy pasture and water mainly have water hyacinth, duckweed, great Piao etc. by on-site inspection.Find that water hyacinth grass carp is relatively liked to eat by investigation, quantity has been lacked and is difficult amount reproduction, quickly, and general medicine can not all be killed the big breeding of quantity, and control is difficult again, it is easy to cause fish anoxic and mortality.Duckweed grass carp Tilapia mossambica all very eat a small amount of it is difficult to breeding, even if put q.s amount reproduction getting up because the smaller density rear in flakes of its individuality is larger, causes oxygen consumption very serious by love.Find that big Piao grass carps are not liked to ingest by site test and a panel region only need to be separated out in the pool, delivered toward the region a small amount of " big Piao ", and in experiments it is found that one kind " medicine " is very sensitive to big Piao, and to fish nonhazardous effect.
Research is found that carbon ammonium and percarbonic acid calcium by weight 5 in pasture and water planting process：1 ratio is mixed, and is locally splashed at pasture and water, and directly pulvis is sprinkling upon on pasture and water, every mu of 5kg.Great Piao are very sensitive to the mixture, and the mixture is especially suitable for controlling the quantity and density of great Piao to fish and water body fanout free region.
It is prepared by the pasture and water of embodiment 2 block
Prior art is blocked using individual layer and directly separates pond, but the trip for being susceptible to pasture and water in SEA LEVEL VARIATION and strong wind weather when finding that monolayer net separates in practical operation is altered.Considering cost and practicality, selection mineral water bottle and mesh bag are fabricated to filter, can not only have been moved up and down with water level but also can be with cost-effective.
It is prepared by the Rhodopseudomonas palustris probiotics of embodiment 3
1. the formula (/L) of Rhodopseudomonas palustris Amplification Culture base：
CaCl20.1g, KH2PO40.5g, NH4Cl 1g, MgCl2·6H2O 0.5g, NaCl 1g, NaAc3H2O 1g, two hydration sodium succinate 1g, dusty yeast 0.3g, peptone 0.3g
2. Amplification Culture base incubation step
(1) each component is weighed according to culture medium prescription, with water dissolves, during culture medium dispensed into the PET bucket disinfected in alcohol to 5L, every barrel of 4.5L is inoculated with Rhodopseudomonas palustris CGMCC No.9136500mL, capping.Incandescent lamp illumination cultivation 4 days in carton are placed in, can be as the seed liquor of next step large-scale culture.
(3) each composition required for weighing 1 ton of nutrient solution, is first completely dissolved with a small amount of running water, then is sufficiently mixed in drum with remaining running water, is inoculated with 10 barrels of photosynthetic bacteria seed liquors, and capping in 5L transparent PET Plastic Drums is sub-packed in after mixing.Illumination cultivation 5 days, treats that culture to rufous, that is, obtains the Rhodopseudomonas palustris probiotics of high-purity.
2. Rhodopseudomonas palustris CGMCC No.9136 are tested the degradation capability of nitrite
(1) nitrite standard curve is done：The nitrite solution of various concentrations is prepared, is placed under ELIASA and is measured OD550, make salinity and OD550Correspondence standard curve.
(2) culture medium of nitrous experiment drops：Prepare 3 ° of salt solution to be sub-packed in the small triangular flasks of 150mL, 50mL/ bottles, every bottle of addition 0.05g sediment of pond.121 DEG C, high pressure steam sterilization 20min.
(3) dark condition and illumination condition are set, experimental group and control group, 3 repetitions of every plant of bacterium is set under the conditions of each, experimental group adds the μ L of photosynthetic bacteria seed liquor 50, is respectively placed under dark and illumination condition, and 30 DEG C are placed culture.
(4) a content of nitrite is measured within every 24 hours, specific method is：1mL liquid is taken in superclean bench in the centrifuge tube of 1.5mL, 12,000rpm are centrifuged 2 minutes, the μ L of supernatant 200 are in 96 orifice plates for absorption, Griess reagent I, II each 20 μ L are successively added, standing 5min makes it react completely, is subsequently placed under ELIASA and measures OD550。
(5) according to standard curve experiment with computing group and the nitrite concentration of control group, measure 2 days, record experimental data and analyze.
2. experimental result
As shown in figure 1, the damp red pseudomonas CGMCC No.9136 microbial inoculums have obvious nitrite degradation effect under dark and illumination condition.Under non-illuminated conditions, nitrous content is degraded to minimum 0.05mg/L from 2.5mg/L；Under illumination condition, natrium nitrosum is degraded to the level of 0.005mg/L from 2.3mg/L.
Embodiment 4
3 mu of pool coverage of water of experiment, 2.5 meters of mean depth, main foster Tilapia mossambica set supports grass carp, and the tail of Tilapia mossambica 7000 is put altogether, and the tail of grass carp 400 separates the water surface 1/4 or so with block, the Zhong Zhi great Piao on 1/4 water surface.Great Piao density is in 70/square metre.In the aquatic products probiotics that summer high temperature season is prepared per first quarter moon with embodiment 3.5 mu of pool coverage of water of control, 2.5 meters of mean depth, main foster Tilapia mossambica nesting grass carp, breeding density is suitable with the experiment pool.The daily feeding volume in two pools is 1.5%, and other conditions are suitable.Temperature and dissolved oxygen (being measured at 30cm under water) and the experiment pool are without physical and chemical index in the water such as dissolved oxygen, temperature, ammonia nitrogen, nitrite at pasture and water at the pool pasture and water of measure examination daily, the experiment specific measurement result in the pool is auspicious to be shown in Table 1, and control pool result of the test refers to table 2.By Tables 1 and 2 it can be seen that when temperature reaches more than 34 DEG C, water temperature can be more than 32 DEG C at the 30cm of underwater, mortality substantially increases, and plants the pond of pasture and water, and the index decline such as ammonia nitrogen, nitrite is very fast in water when water temperature typically can all compare photograph low 2-3 DEG C of the pool and fine day at pasture and water.
Measurement result is surveyed on the experiment of table 1 pool
(note：Experiment dissolved oxygen is dissolved oxygen at experiment pool pasture and water；Test temperature is temperature at pasture and water；Dissolved oxygen is the experiment pool without temperature at pasture and water；Temperature is the experiment pool without temperature at pasture and water)
Table 2 compares pool measurement result
Interpretation of result
1st, test pool temperature and compare pool temperature change relation
The experiment pool and control pool temperature change with time as shown in Figure 2.It can be seen that there is the pond that pasture and water cover in August 19 days to August 24 days and in September 5 days to the degree/day of September 10 significantly lower than 2-3 DEG C of the pool of control, August 25-9 months 26 degree/day it is suitable (being primarily due to this time mostly rainy weather).Thus provable, the pasture and water that 1/4 area is planted in pond can effectively reduce 2-3 DEG C of pond water temperature in fine day high temperature season, and then can alleviate the generation of high temperature season Tilapia mossambica hyperpyrexia disease.
2nd, test pool dissolved oxygen and compare the variation relation between the dissolved oxygen of the pool
The experiment pool and control pool dissolved oxygen change with time as shown in Figure 3.Result shows, experiment pool dissolved oxygen (without pasture and water part) with compare pool dissolved oxygen and be more or less the same, amplitude range is between 0.2-1mg/L, and dissolved oxygen is all in more than 3mg/L, it was demonstrated that little on the dissolved oxygen influence in water body when pasture and water area coverage is 1/4.
3rd, experiment pool ammonia nitrogen, nitrite, pH and the changes in contrast result for compareing the pool
Test the ammonia nitrogen in the water of the pool, nitrite, be decreased obviously compared with control group after the probiotics with the use of independent research, illustrate that pasture and water are planted in the pool has good adjustment effect with the use of probiotics to ammonia nitrogen, nitrite in water, the immunity of fish can be significantly improved, the generation of hammer disease is effectively prevented.
By the experiment for scheduling to last nearly month, originally grass carp, Tilapia mossambica have been determined and have not liked the pasture and water " big Piao " for ingesting; have selected the medicine (pulvis carbon ammonium+percarbonic acid calcium) that pasture and water are killed in control; drawn pasture and water can reduce water when pond 1/4 is covered in 2-3 DEG C of temperature; dissolved oxygen reduction 0.5-1mg/L, mortality can obviously reduce.In terms of expense, the experimental expenses at each foster family is amounted to no more than 2500 yuan, if promoting this aquaculture model, maintenance can provide the expenses such as waste product mineral water bottle etc., comprehensive accounting pasture and water control medicine, mesh bag for oneself, every 5 mu of pools are no more than 300 yuan, possess the possibility of large-scale promotion.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, on the premise of the technology of the present invention principle is not departed from; some improvements and modifications can also be made, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (5)
1. a kind of grass carp and Tilapia mossambica ecological polyculturing method, it comprises the following steps：
1) culturing pool selection：Selection depth more than 2m, area is 2-3 mus of pond, uses filter
Separate the area of 1/4-1/3, in high temperature season, when water temperature is more than more than 30 DEG C, forbid duck excrement,
Pig manure water enters the pool；
2) pasture and water are planted：The Zhong Zhi great Piao in the pond that filter separates, it is 60-80 to control great Piao density
/ square meter；Control pond is partly dissolved oxygen in more than 3mg/L without pasture and water, in the middle of one day most
High-temperature control at pasture and water temperature below 32 DEG C；
3) an aquatic products probiotics is applied per first quarter moon in high temperature season.
2. ecological polyculturing method as claimed in claim 1, it is characterised in that described water
Probiotics is produced by Rhodopseudomonas palustris Rhodopseudomonas palustris
CGMCC No.9136 fermentations are formed.
3. ecological polyculturing method as claimed in claim 1, it is characterised in that pond culture
Density is every mu of 100-200 tail of grass carp, every mu of 2000-3000 tail of Tilapia mossambica.
4. ecological polyculturing method as claimed in claim 1, it is characterised in that by such as lower section
The density of method control great Piao：By carbon ammonium and percarbonic acid calcium by weight 5：1 ratio is mixed
Close, locally splashed at pasture and water, directly pulvis is sprinkling upon on pasture and water, every mu of 5-10kg.
5. ecological polyculturing method as claimed in claim 1, it is characterised in that the filter
Enter mesh bag with empty Plastic bottle plug to be obtained.
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| CN102461472A (en) * | 2010-11-16 | 2012-05-23 | 柳州市鱼家乐饲料有限公司 | Tilapia breeding method |
| CN102986735A (en) * | 2012-12-10 | 2013-03-27 | 骆世鸿 | Efficient environment-friendly water lettuce prevention and control medicine and preparation method and application method thereof |
| CN103314896A (en) * | 2013-06-17 | 2013-09-25 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Intercropping method for finless eel and mud fish in lotus root pond |
| CN103436472A (en) * | 2013-08-23 | 2013-12-11 | 福州大用生物应用科技有限公司 | Composite micro-ecological preparation for improving pond water quality |
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| CN102461472A (en) * | 2010-11-16 | 2012-05-23 | 柳州市鱼家乐饲料有限公司 | Tilapia breeding method |
| CN102986735A (en) * | 2012-12-10 | 2013-03-27 | 骆世鸿 | Efficient environment-friendly water lettuce prevention and control medicine and preparation method and application method thereof |
| CN103314896A (en) * | 2013-06-17 | 2013-09-25 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Intercropping method for finless eel and mud fish in lotus root pond |
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Address after: 363502 Jindu Industrial Concentration Zone, Zhaoan County, Zhangzhou City, Fujian Province Patentee after: Fujian Dabei nonghuayou Aquatic Technology Group Co.,Ltd. Patentee after: BEIJING DABEINONG TECHNOLOGY GROUP Co.,Ltd. Address before: 363502 Jindu Industrial Concentration Zone, Zhaoan County, Zhangzhou City, Fujian Province Patentee before: FUJIAN DABEINONG FISHERIES SCIENCE & TECHNOLOGY Co.,Ltd. Patentee before: BEIJING DABEINONG TECHNOLOGY GROUP Co.,Ltd. |
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